• Glucokinase plays an important role in the regulation of carbohydrate metabolism. (generalpatent.com)
  • IL-1 beta functions in a central role in immune and inflammatory responses, bone remodeling, fever, carbohydrate metabolism, and GH/IGF-I physiology. (rndsystems.com)
  • A multi-institutional study led by Dr. Tadashi Takeuchi at Stanford University and published in Nature , demonstrates the specific role that various species within the gut microbiota play in carbohydrate metabolism, suggesting the potential for insulin-sensitive (IS) gut bacteria to combat insulin resistance (IR) and serve as a treatment for individuals with Type 2 diabetes. (technologynetworks.com)
  • Previous studies have indicated that many species in the gut microbiota are involved in carbohydrate metabolism, which can contribute to insulin resistance when unregulated. (technologynetworks.com)
  • Although carbohydrate metabolism has been implicated in obesity and prediabetes before, the actual biological link has not been thoroughly studied, and Takeuchi et al. (technologynetworks.com)
  • We used whole-transcriptome shotgun sequencing (RNA-seq) to compare the S. pneumoniae transcriptome in biofilms, bacteria dispersed from biofilms after exposure to IAV, febrile-range temperature, or ATP, and planktonic cells grown at 37°C. Compared with biofilm bacteria, actively dispersed S. pneumoniae, which were more virulent in invasive disease, upregulated genes involved in carbohydrate metabolism. (lu.se)
  • Chromium is considered both an essential element for carbohydrate metabolism and toxic (Cefalu et al. (cdc.gov)
  • Bacteroides, Ruminococcaceae, Faecalibacterium prausnitzii) and three Kyoto Encyclopedia of Genes and Genomes orthologues, of which two relate to carbohydrate metabolism. (medscape.com)
  • Bexsero Antigen Sequence Types (BASTs) were used to analyze whole-genome sequences of 3,073 prevaccine IMD N. meningitidis isolates obtained during 2010−2016. (cdc.gov)
  • BAST surveillance of meningococcal whole-genome sequence data is rapid, scalable, and portable and enables international comparisons of isolates. (cdc.gov)
  • In the current release of the PGG.Han database, we collect data of 114,783 individuals in total, including whole-genome sequencing data of 12,197 samples and genome-wide single nucleotide polymorphism (SNP) data of 102,586 samples. (biosino.org)
  • With these more than 100K Han Chinese samples being whole-genome sequenced to high coverage (~30 - 80×), or low-coverage (~1.7 - 4×), or high-density genome-wide SNP genotyped or imputed, we constructed the first and the largest reference panel specific to the Han Chinese population, a.k.a. (biosino.org)
  • Using all the whole-genome sequencing data of Han Chinese samples as reference data, the 102,586 samples are carefully imputed so that the final data set 8,056,973 genome-wide SNPs. (biosino.org)
  • Rapid Whole Genome Sequencing Diagnoses and Guides Treatment in Critically Ill Children in Belgium in Less than 40 Hours. (cdc.gov)
  • Despite the role that the human gut microbiota plays in health and diseases, little is known about its composition and functional capacity during prolonged fasting when the external nutrient supply is reduced or suppressed. (nih.gov)
  • Dietary supplementation with complex carbohydrates is known to alter the composition of gut microbiota, and optimal implementation of the use of these so called "prebiotics" could be of great potential in prevention and possibly treatment of obesity and associated cardiometabolic and inflammatory diseases via changes in the gut microbiota. (frontiersin.org)
  • An alternative to this "microbiocentric view" is the idea that health-promoting effects of certain complex carbohydrates reside in the host, and could secondarily affect the diversity and abundance of gut microbiota. (frontiersin.org)
  • Feeding xanthan gum to germfree mice colonized with a human microbiota containing the uncultured Ruminococcaceae supports the idea that the additive xanthan gum can drive expansion of the primary degrader Ruminococcaceae, along with exogenously introduced B. intestinalis . (nature.com)
  • I got really interested in not just looking at proteins as these isolated things, [where] we pull out the sequence [and] look at the structure, but more about how they were contributing to the whole organism, and to the larger microbiota as well," she shared. (asm.org)
  • Several mutations in the N terminus of the G-protein-coupled receptor rhodopsin disrupt NXS/T consensus sequences for N-linked glycosylation (located at N2 and N15) and cause sector retinitis pigmentosa in which the inferior retina preferentially degenerates. (jneurosci.org)
  • Here we examined the role of rhodopsin glycosylation in biosynthesis, trafficking, and retinal degeneration (RD) using transgenic Xenopus laevis expressing glycosylation-defective human rhodopsin mutants. (jneurosci.org)
  • Together, the human disease and animal models suggest that glycosylation plays a crucial role in the structure and/or function of rhodopsin. (jneurosci.org)
  • NMR investigations of the N-linked oligosaccharides at individual glycosylation sites of human lutropin. (wikipedia.org)
  • Faecal 16S rRNA gene amplicon sequencing showed that fasting caused a decrease in the abundance of bacteria known to degrade dietary polysaccharides such as Lachnospiraceae and Ruminococcaceae. (nih.gov)
  • Analysis of their deduced amino acid sequences revealed several important differences from prototypic subtype B strains, including changes in the number and distribution of cysteine residues, substantial length differences in hypervariable regions, and premature truncations in the gp41 domain. (duke.edu)
  • Homo sapiens carbohydrate sulfotransferase 9 (CHST9), transcript variant 1, mRNA. (genscript.com)
  • random to their competing genomic online artificial risk and the attP of the such trial in suppressor target pIRES2-EGFP-TSLC1 trait4 residues are again associated for the conditionsPrivacy of sequences for collection with a mRNA of elsewhere 50 polynucleotide among the polynomial differences( Figure 1). (scoutconnection.com)
  • Aberrant methylation patterns were inversely correlated with gene expression changes using mRNA sequencing from same animals and tissue specimens. (uni-koeln.de)
  • Human Microbiome Project Consortium. (nature.com)
  • Structure, function and diversity of the healthy human microbiome. (nature.com)
  • To circumvent this potential interpretational problem, we aimed at providing an overview about whether and how dietary supplementation of different complex carbohydrates changes the gut microbiome in healthy non-obese individuals. (frontiersin.org)
  • We then reviewed whether the reported changes in gut bacterial members found to be established by complex carbohydrates would benefit or harm the cardiometabolic and immunological health of the host taking into account the alterations in the microbiome composition and abundance known to be associated with obesity and its associated disorders. (frontiersin.org)
  • Because certain complex carbohydrates also affect the host's immunity directly, it is likely that host-microbiome interactions in determination of health and disease characteristics are indeed bidirectional. (frontiersin.org)
  • Overall, this review article shows that whereas it is relatively clear in which direction supplemental fermentable carbohydrates can alter the gut microbiome, the relevance of these changes regarding health remains controversial. (frontiersin.org)
  • While the human intestinal microbiome is dominated by the phyla Bacteroidetes and Firmicutes (representing more than 90% of the total bacterial population) and with Proteobacteria, Verrucomicrobia, Actinobacteria, Fusobacteria, and Cyanobacteria generally being present in minor proportions ( 6 ), the exact composition can vary widely between individuals. (frontiersin.org)
  • After graduating in 2011, Pollet pursued her Ph.D. at University of North Carolina Chapel Hill in the structural biochemistry lab of Matthew Redinbo, Ph.D . Her doctoral work focused on characterizing microbial proteins involved in antibiotic resistance , as well as β-glucuronidases (enzymes that break down complex carbohydrates) in the human microbiome. (asm.org)
  • Human Molecular Genetics. (wikipedia.org)
  • The 17 kDa molecular weight mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta. (rndsystems.com)
  • Molecular cloning of the human thyrotropin-beta subunit gene. (wikipedia.org)
  • Science: molecular biology) a base sequence in a dna molecule (or an amino acid sequence in a protein) that has remained essentially unchanged throughout evolution . (biologyonline.com)
  • Scholars@Duke publication: Molecular cloning and analysis of functional envelope genes from human immunodeficiency virus type 1 sequence subtypes A through G. The WHO and NIAID Networks for HIV Isolation and Characterization. (duke.edu)
  • By combining these research areas, we aimed to give a better insight into the potential of (foods containing) complex carbohydrates in the treatment and prevention of above-mentioned diseases. (frontiersin.org)
  • We conclude that supplemental complex carbohydrates that increase Bifidobacteria and Lactobacilli, without increasing the deleterious Bacteroides , are most likely promoting cardiometabolic and immunological health in obese subjects. (frontiersin.org)
  • It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). (rndsystems.com)
  • Obese, hypertensive, type 2 diabetic rats SHR/NDmcr-cp were given, for 12 weeks, either a normal, middle-carbohydrate/middle-fat diet (MC/MF group) or a high-carbohydrate/low-fat diet (HC/LF group). (hindawi.com)
  • Altogether, these data identify a specific role of body weight control by a high-carbohydrate/low-fat diet in the progression of DN. (hindawi.com)
  • The observation that a high-carbohydrate/low-fat diet reduces effectively body weight without calorie restriction [ 13 , 14 ] led us to use a similar isocaloric diet in our rat model to better identify factors mediating the weight-related factors involved in the genesis of DN. (hindawi.com)
  • SHR/NDmcr-cp rats given a normal middle-carbohydrate/middle-fat diet (MC/MF group) were thus compared with similar rats fed a high-carbohydrate/low-fat diet (HC/LF group). (hindawi.com)
  • SHR/NDmcr-cp rats, aged 5 weeks, were randomly divided into two groups and given for 12 weeks either a normal diet (CE-2, CLEA Japan Inc., Tokyo, Japan) with tap water (MC/MF group, 10 rats), or a high carbohydrate/low fat diet (CE-2 with tap water containing 30% sucrose) (HC/LF group, 10 rats). (hindawi.com)
  • Gary F. Clark, PhD, is a researcher whose primary area of interest is glycobiology - the study of the structure, function and biology of carbohydrate sequences (glycans). (missouri.edu)
  • N-linked glycans are commonly found in the complex cells of humans and other animals. (nist.gov)
  • N-linked glycans are commonly found on proteins, but always on a specific amino acid within a specific sequence. (nist.gov)
  • These are the precursors, cores and internal sequences of N-glycans. (longdom.org)
  • It is now well recognized that the bacteria living in our gut play an important role in the development and maintenance of the innate and adaptive immune system ( 1 , 2 ) as well as the fermentation of low-, or non-digestible dietary carbohydrates ( 3 - 5 ). (frontiersin.org)
  • Our work demonstrates the existence of a potential xanthan gum food chain involving at least two members of different phyla of gut bacteria and provides an initial framework for understanding how widespread consumption of a recently introduced food additive influences human microbiomes. (nature.com)
  • They analyzed metabolites in 22 human fecal IS- and IR-associated bacteria to understand which carbohydrates were consumed by each type of bacteria. (technologynetworks.com)
  • We used whole-transcriptome shotgun sequencing (RNA-seq) to compare the S. pneumoniae transcriptome in biofilms, bacteria dispersed from biofilms after exposure to IAV, febrile-range temperature, or ATP, and planktonic cells grown at 37°C. Compared with biofilm. (lu.se)
  • The PAP20 glycopeptides were isolated by reversed phase high pressure liquid chromatography and subjected to the structural analyses by quadrupole time-of-flight electrospray ionization mass spectrometry and to the sequencing by Edman degradation. (nih.gov)
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  • The following CHST9 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). (genscript.com)
  • These sequences represent the protein coding region of the CHST9 cDNA ORF which is encoded by the open reading frame (ORF) sequence. (genscript.com)
  • Isolation, cloning and sequence analysis of the cDNA for the alpha-subunit of human chorionic gonadotropin. (wikipedia.org)
  • The new patent claims a method of detection of specified sequences from polynucleotide sequence which encodes a protein that has human glucokinase activity. (generalpatent.com)
  • This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. (wikipedia.org)
  • To generate a more representative panel of genetically diverse envelope genes, we PCR amplified, cloned, and sequenced complete gp160 coding regions of 35 primary (peripheral blood mononuclear cell-propagated) HIV-1 isolates collected at major epicenters of the current AIDS pandemic. (duke.edu)
  • 90 samples (overlapping with CHB and CHS in the 1000 Genomes Projects) were sequenced by BGI-Shenzhen at a high depth (~80×) (Lan et al. (biosino.org)
  • The genomes of 11,670 women Han Chinese were sequenced by the CONVERGE project as a control group for the investigation of major depressive disorders (Chiang et al. (biosino.org)
  • Using a new approach to reconstitute antigen-receptor interactions in a human reporter B cell line, we found that sequence-defined BCRs from the human germline repertoire could be triggered by both complementarity to influenza HA and a separate mode of signaling that relied on multivalent ligation of BCR sialyl-oligosaccharide. (harvard.edu)
  • different online artificial receptor, longitudinal property and 0K1hisds+∫ backbone of sequence restriction of areas,4,368 data: pFlpBtM-II for expression Steroid, 2000 Mol. (scoutconnection.com)
  • Given the sequence similarity and overlap in receptor binding, the literature for these derivative peptides is relevant for the consideration of frag 176-191 and will be referred to interchangeably throughout this article. (outlookindia.com)
  • METHODS: Relevant regions of the GLP-2 receptor gene were sequenced. (bvsalud.org)
  • According to the European Medicines Agency guideline on environmental risk assessments for pharmaceuticals (EMA/CHMP/SWP/4447/00) vitamins, electrolytes, amino acids, peptides, proteins, carbohydrates, lipids, vaccines and herbal medicinal products are exempted because they are unlikely to result in significant risk to the environment. (janusinfo.se)
  • Pollet developed a particular fascination with protein biochemistry after learning about the importance of proteins in cellular functions, and how a simple tweak in the DNA sequence and structure of a protein could lead to disease. (asm.org)
  • Proteins are molecules that consist of amino acids, and nine of these are considered essential because they cannot be manufactured by the human body. (themanual.com)
  • For diet-driven alterations of gut microbial composition particularly the type and proportion of complex indigestible carbohydrates that reach the colon turn out to be of great importance ( 18 , 19 , 21 - 24 ). (frontiersin.org)
  • Administration of a ketogenic, high-fat, low-carbohydrate diet attenuated seizure progression and ameliorated DNA methylation mediated changes in gene expression. (uni-koeln.de)
  • Intervention in to human diet. (who.int)
  • Plato suggested that the therapeu- tory of St. Bartholomew's Hospital in Lon- tic approach to human diseases should be don, the oldest British hospital, established made through the regulation of diet rather in 1123 [ 5 ]. (who.int)
  • Suffern, N.Y. -August 27, 2014 - Ryogen LLC, an R&D company focusing on polynucleotide sequences implicated in human diseases, was awarded two new patents, bringing the total number of its issued U.S. Patents to 34. (generalpatent.com)
  • Gaussian online artificial photosynthesis in Human Cells Catalyzed by Phage polynucleotide Integrase Mutants, 2000 J. Maeser and Kahmann, The Gin model of event Mu can promote supernatant health in spline pairs, 1991 Mol. (scoutconnection.com)
  • Previous studies in animal models as well as human epileptic brain tissue revealed a remarkably diverse pattern of gene expression implicating epigenetic changes to contribute to disease progression. (uni-koeln.de)
  • Present knowledge of human immunodeficiency virus type 1 (HIV-1) envelope immunobiology has been derived almost exclusively from analyses of subtype B viruses, yet such viruses represent only a minority of strains currently spreading worldwide. (duke.edu)
  • Phylogenetic analyses of full-length env sequences indicated that the panel included members of all major sequence subtypes of HIV-1 group M (clades A to G), as well as an intersubtype recombinant (F/B) from an infected individual in Brazil. (duke.edu)
  • Human mannose-binding protein carbohydrate recognition domain trimerizes through a triple α-helical coiled-coil. (lu.se)
  • Genotype or clonal complex (CC), identified by multilocus sequence typing (MLST), groups related organisms and is useful for categorizing IMD phenotype, antimicrobial drug resistance, and vaccine antigens ( 8 , 9 ). (cdc.gov)
  • Michael Breimer started his research in the Department of Medical and Physiological Chemistry isolating and structurally characterising blood group AB(O)H and related carbohydrate antigens. (gu.se)
  • The digestibility of dietary carbohydrates depends, among others, on structural properties of the carbohydrate, such as the degree of polymerization (DP) and branching. (frontiersin.org)
  • However, serogroup B capsular polysaccharides are poorly immunogenic and share structural similarity to carbohydrates found in human tissues ( 6 ). (cdc.gov)
  • Between the cells of the basal layer of the human epidermis, there are melanocytes, which contain tyrosinase that can oxidize tyrosine into polysaccharides, and then go through a series of metabolic processes in the middle to finally generate melanin. (zhenyibio.com)
  • The high incidence of these replacements and their detection also in other cancer cell lines imply that the conserved tandem repeat domain of MUC1 is polymorphic with respect to the peptide sequence. (nih.gov)
  • Its peptide sequence contains 2 tyrosines, 2 arginines and 3 serines. (zhenyibio.com)
  • Serum biochemistry confirmed the metabolic switch from carbohydrates to fatty acids and ketones. (nih.gov)
  • Baseline gut microbial composition (16S rRNA sequencing) and metabolic capacity (PICRUSt) were determined. (medscape.com)
  • Strong anion exchange-mediated phosphoproteomics reveals extensive human non-canonical phosphorylation. (liverpool.ac.uk)
  • Graduates of these programs work in such areas as pharmaceuticals and related human health activities, food science and nutritional biochemistry, environment, energy, as well as biotechnological related issues of finance and policy. (brocku.ca)
  • He has published more than 150 scientific articles in the fields of carbohydrate biochemistry, glycobiology, allo- and xeno-transplantation, and regeneratvie medicine. (gu.se)
  • Intended Use Human CHST3 ELISA Kit allows for the in vitro quantitative determination of CHST3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids. (biobool.com)
  • We report here a carbohydrate microarray analysis of a number of tumor-associated carbohydrates for their serum antibody reactivities and potential immunogenicity in humans. (longdom.org)
  • Human peripheral blood mononuclear cells treated with LPS and fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). (rndsystems.com)
  • Another hGH fragment named AOD9604 has received the most attention, and is nearly identical to frag 176-191, as both peptides share the same amino acid sequence from positions 177 to 191. (outlookindia.com)
  • T47-D cell lysate, colon cancer lysate, Human breast carcinoma tissue, Human normal breast tissue. (abcam.com)
  • Human PBMC stimulated with 250 ng/ml LPS overnight were stained with either (A) Mouse Anti-Human IL-1 beta /IL-1F2 Alexa Fluor® 594-conjugated Monoclonal Antibody (Catalog # IC201T) or (B) Mouse IgG1Isotype Control ( IC002T ) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody ( FAB3832A ). (rndsystems.com)
  • Carbohydrate xenoantigens and xenoantibody epitope-specificity, the removal of xenoantibodies by plasmapheresis, and antibody neutralization using soluble saccharides, were all explored. (gu.se)
  • CD62L binds to carbohydrates present on certain glycoforms of CD34, glycam-1, and MAdCAM-1 and with a low affinity to anionic oligosaccharide sequences related to sialylated Lewis X (sLex, CD15s) through its C-type lectin domain. (biolegend.com)
  • Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). (rndsystems.com)
  • However, for thyriothecial species, phylogenies from DNA sequence data and illustrations of scutella remain have been limited. (ubc.ca)
  • Of the delimited species, nine had been collected ten or more times each and were, based on morphology and sequence analysis, undescribed and restricted to North America. (ubc.ca)
  • I sampled 713 ITS2 sequences of American Pacific Northwest specimens collected by Seattle architect Benjamin Woo (1923-2008). (ubc.ca)
  • Sequences for all of the known enzymes involved in TIAs synthesis were identified. (biomedcentral.com)
  • In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human (rh) Resistin is observed. (rndsystems.com)
  • RGB-10, as teriparatide is the active substance, is a recombinant human peptide and therefore not expected to pose a risk to the environment.The Applicant provided an appropriate justification for not submitting an Environmental Risk Assessment, as postulated in the CHMP guideline on the environmental risk assessment of medicinal products for human use (EMEA/CHMP/SWP/4447/00). (janusinfo.se)
  • B cell superantigen activity through affinity for BCR carbohydrate is discussed. (harvard.edu)
  • Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16). (rndsystems.com)
  • 2002). „Assessment of the in vitro and in vivo biological activities of the human follicle-stimulating isohormones. (wikipedia.org)
  • Specificity This assay has high sensitivity and excellent specificity for detection of human CHST3. (biobool.com)
  • They are generated by alternative splicing combinations of three exons (A, B, and C) of the genomic sequence. (beckman.com)
  • Using methyl-CpG capture associated with massive parallel sequencing (Methyl-Seq) we report the genomic methylation signature of the chronic epileptic state. (uni-koeln.de)
  • The non-restricted CD45, Leucocyte Common Antigen (LCA) consists of an extracellular sequence, proximal to the membrane, which is common to all CD45 isoforms. (beckman.com)
  • Economic Evaluation of Next-generation Sequencing Techniques in Diagnosis of Genetic Disorders: A Systematic Review. (cdc.gov)
  • Targeted Next-Generation Sequencing in Children With Bilateral Sensorineural Hearing Loss: Diagnostic Yield and Predictors of a Genetic Cause. (cdc.gov)
  • Genetic sequences of the bases are read in groups of three (called a triplet), with a possibility of 64 configurations or "words" in which to code information. (cdc.gov)
  • Detection of IL‑1 beta /IL‑1F2 in Human PBMC by Flow Cytometry. (rndsystems.com)
  • Sensitivity The minimum detectable dose of human CHST3 is typically less than 6.25 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. (biobool.com)
  • Detection of sensations allow the human physique to concentrate on changes (or stimuli) that occur in the surroundings or contained in the body. (ehd.org)
  • No significant cross-reactivity or interference between human CHST3 and analogues was observed. (biobool.com)
  • CONCLUSIONS: Biomarkers predicting clinical and functional features in human SBS are very limited. (bvsalud.org)
  • The protein name derives from the protein's interaction with carbohydrate response element sequences of DNA. (wikipedia.org)
  • Moreover, transiently expressed glycoprotein precursor molecules varied considerably in both size and carbohydrate content. (duke.edu)
  • Primary amino acid sequence of follicle-stimulating hormone from human pituitary glands. (wikipedia.org)
  • 1990). „Crystallization and characterization of human chorionic gonadotropin in chemically deglycosylated and enzymatically desialylated states. (wikipedia.org)
  • However, viral expression of these carbohydrates may trigger host immune responses. (longdom.org)
  • During more recent years, his reseach has been conducted in the field of regenerative medicine, focussed on antigen expression and immune recognition of human embryonic stem cells and bioartificial heart valves, the latter tissues being of animal origin. (gu.se)
  • Scott, G.R. & Poulson, S.R. Stable carbon and nitrogen isotopes of human dental calculus: a potentially new non-destructive proxy for paleodietary analysis. (nature.com)
  • 1989). „Preliminary X-ray diffraction analysis of human chorionic gonadotropin. (wikipedia.org)
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  • ORF sequences can be delivered in our standard vector, pcDNA3.1 + /C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. (genscript.com)
  • Human peripheral lymphocytes were stained with anti-human 62L (clone DREG-56) APC/Fire™ 810 (filled histogram) or mouse IgG1, ĸ APC/Fire™ 810 isotype control (open histogram). (biolegend.com)