Loading...
  • metabolites
  • Possible vasoconstrictor mediators include arachidonic acid-derived lipoxygenase (LO) metabolites. (ahajournals.org)
  • Both of these metabolites, depending on the cell type which forms them, can be further metabolized to 15-oxo-eicosatetraenoic acid (15-oxo-ETE), 5S,15S-dihydroxy-eicosatetraenoic acid (5(S),15(S)-diHETE), 5-oxo-15(S)-hydroxyeicosatetraenoic acid (5-oxo-15(S)-HETE, a subset of specialized pro-resolving mediators viz. (wikipedia.org)
  • 15(S)-HETE, 15(S)-HpETE, and many of their derivative metabolites are thought to have physiologically important functions. (wikipedia.org)
  • It therefore forms linoleic acid metabolites (e.g. 13-hydoxyperoxy/hydroxy-octadecadienoic and 9-hydroperoxy/hydroxyl-octadecadienoic acids) in greater amounts than 15(S)-HpETE and 15(S)-HETE. (wikipedia.org)
  • In general, various phospholipase A2s and diacylglycerol lipases are activated during cell stimulation, proceed to release these fatty acids from their storage sites, and thereby are key regulators in the formation of lipoxygenase-dependent metabolites. (wikipedia.org)
  • 12-Lipoxygenase (12-LO, gene expressed as ALOX12 in humans and 12-Lo in rodents in this manuscript) produces proinflammatory metabolites such as 12(S)-hydroxyeicosatetraenoic acids through dioxygenation of polyunsaturated fatty acids. (paperity.org)
  • A closely related set of 15-lipoxygenase metabolites are derived from anandamide (i.e. arachidonic acid containing ethanolamine esterified to its carboxy residue). (wikipedia.org)
  • Although most attention has focused on PGs 2 and other COX-derived metabolites, mounting evidence suggests that LO-catalyzed products, LTs, and HETEs also exert profound biological effects on the development and progression of human cancers. (aacrjournals.org)
  • pathways
  • In addition, cells, when so activated, may transfer their released polyunsaturated fatty acids to adjacent or nearby cells which then metabolize them through their lipoxygenase pathways in a process termed transcellular metabolism or transcellular biosynthesis. (wikipedia.org)
  • Human aortic endothelial cells cultured in chronically high glucose levels results in elevated levels of 12S-HETE, suggesting activation of lipoxygenase pathways [ 12 , 13 ]. (hindawi.com)
  • It was previously shown that lipoxygenase (LO) pathways are important in the rheumatoid arthritis (RA) inflammatory process and that synovial fluid from RA patients contains high amounts of leukotrienes. (biomedcentral.com)
  • pathway
  • Most notably, 5-lipoxygenase (LO) pathway products including LTC 4 and -D 4 , the slow-reacting substances of anaphylaxis, are considered causative agents in airway allergic responses, because they are potent bronchoconstrictors and inducers of leakage permeability changes ( 2 , 3 ). (jimmunol.org)
  • Results suggest that the 15-LO pathway is different between females and males and is regulated by estrogen. (ahajournals.org)
  • Separate lines of research have identified the 5-lipoxygenase/leukotriene B 4 receptor pathway and the PPAR pathway as potential targets for prevention or treatment of this disease. (hindawi.com)
  • Genes of the 5-lipoxygenase pathway are compelling candidates for atherosclerosis. (hindawi.com)
  • Further, lipoxygenase pathway SNPs that were associated with measures of atherosclerosis were associated with markers of inflammation (CRP, ICAM-1) and calcification (MGP). (hindawi.com)
  • These results suggest that variants in lipoxygenase pathway genes may have pleiotropic effects on multiple components that determine risk of cardiovascular disease. (hindawi.com)
  • The current research was motivated by the role of lipoxygenase pathway gene products in inflammation, initiation/progression of atherosclerosis, and their modulation of expression by glucose. (hindawi.com)
  • The 14,15-HxA3 and 14,15-HxB3 non-classical eicosanoids are distinguished from the aforementioned hepoxilins in that they are formed by a different metabolic pathway and differ in the positioning of their epoxide and hydroxyl residues. (wikipedia.org)
  • mRNA
  • Based predominantly on the presence of its mRNA, human ALOX12 is distributed predominantly in blood platelets and leukocytes and at lower levels in the basal layer of the epidermis (particularly in the skin lesions of psoriasis), islets of Langerhans within the pancreas, and certain cancers. (wikipedia.org)
  • 1999
  • Kelavkar and Badr (1999) described experiments yielding data that supported the hypothesis that loss of the TP53 gene, or gain-of-function activities resulting from the expression of its mutant forms, regulates ALOX15 promoter activity in human and in mouse, albeit in directionally opposite manners. (wikipedia.org)
  • Kelavkar and Badr (1999) referred to this as evidence that 15-lipoxygenase is a mutator gene. (wikipedia.org)
  • type
  • Arachidonate 15-lipoxygenase type B knockdown leads to reduced lipid accumulation and inflammation in atherosclerosis. (biomedsearch.com)
  • In this study, we examined transgenic (TG) rabbits overexpressing 15-LO type I and their response to inflammatory challenge. (jimmunol.org)
  • These results indicate that overexpression of 15-LO type I and LXA 4 is associated with dampened PMN-mediated tissue degradation and bone loss, suggesting that enhanced anti-inflammation status is an active process. (jimmunol.org)
  • In humans, 12-LO expression is increased in pancreatic islets of autoantibody-positive, type 1 diabetic and type 2 diabetic organ donors. (paperity.org)
  • Interestingly, the high expression of ALOX12 is associated with the alteration in first-phase glucose-stimulated insulin secretion in human type 2 diabetic islets. (paperity.org)
  • Indeed, mice made deficient of 12/15-lipooxygenase exhibit an attenuated allergic airway inflammation response compared to wild type control mice. (wikipedia.org)
  • These findings indicated that the 12-lipoxygenase of human uterine cervix was of platelet-type. (nii.ac.jp)
  • Publications] M.Nakamura: 'Immunocytochemical localization of platelet-type arachidonate 12-lipoxygenase in mouse blood cells' J.Histochem.Cytochem.43. (nii.ac.jp)
  • Mice
  • Studies on the role of ALOX12 in pathophysiology using the main models for such functional studies, rats and mice, are complicated because neither species possesses a lipoxygenase that makes a predominance of 12(S)-HETE and therefore is metabolically equivalent to ALOX12. (wikipedia.org)
  • leukotrienes
  • LY293111 was also known to have inhibitory effects on 5-lipoxygenase, which is upstream of the production of leukotrienes. (hindawi.com)
  • That is, the eoxins are 14,15-disubstituted analogs of the 5,6-disubstituted leukotrienes. (wikipedia.org)
  • cells
  • This initial hydroperoxide product is extremely short-lived in cells: if not otherwise metabolized, it is rapidly reduced to 15(S)-HETE. (wikipedia.org)
  • it also promotes the malignant behavior of cultured human cancer cells as well as the growth of certain cancers in animal models (see 12-HETE). (wikipedia.org)
  • They are produced by human eosinophils (a class of white blood cells), mast cells, the L1236 Reed-Sternberg cell line derived from Hodgkin's Lymphoma, and certain other tissues tissue. (wikipedia.org)
  • Cells and tissues rich in 15-LOX-1 activity such as human eosinophils, umbilical cord-derived mast cells, nasal polyps from allergic subjects, airway epitheleal cells, and L1236 Reed-Sternberg cells derived from Hodgkin's Disease tumors produce eoxins. (wikipedia.org)
  • lacking 15-LOX-1, it is assumed that these cells employ 12/15-lipoxygenase to initiate this synthesis. (wikipedia.org)
  • 1) Transcriptional regulation of 12-lipoxygenase : As examined by the luciferase assay, a negative regulatory region including the NFkB motif was found in the 5'-flanking region of the 12-lipoxygenase gene in human erythroleukemia cells. (nii.ac.jp)
  • 3) 12-Lipoxygenase of human uterine cervix : Nigam's group of Free University of Berlin had found that human uterine cervix had 12-lipoxygenase activity which was lower in cancer cells. (nii.ac.jp)
  • Hepoxilins have various biological activities in animal models and/or cultured mammalian (including human) tissues and cells. (wikipedia.org)
  • 5-LO and 15-LO-1 are present in RA and OA synovium, with 5-LO being mostly expressed in lining and sublining macrophages, neutrophils and mast cells and 15-LO-1 mainly in lining macrophages, fibroblasts and sublining endothelial cells. (biomedcentral.com)
  • Also, SF cells express a functional 15-LO-1 and produce 15-HETE when challenged with AA. (biomedcentral.com)
  • Inflammation
  • 15-LO TG rabbits exhibited markedly reduced bone loss and local inflammation. (jimmunol.org)
  • Based on these studies, it has been proposed that the hepoxilins and trioxilins function in human physiology and pathology by, for example, promoting inflammation responses and dilating arteries to regulate regional blood flow and blood pressure. (wikipedia.org)
  • cyclooxygenase
  • 6 The combined cyclooxygenase/lipoxygenase (LO) inhibitor BW 755C completely blocked contractions in the females. (ahajournals.org)
  • Omega 3 fatty acids inhibit tumor growth by a number of mechanisms including suppression of COX-2 expression and, for EPA at least, the alternative substrate produces different cyclooxygenase (PGE 3 ) and lipoxygenase (LTB 5 ) products that have anti-inflammatory and anticancer effects. (hindawi.com)
  • eicosanoids
  • 2. The method of claim 1 , wherein modulating the biological availability of eicosanoids in a subject comprises reducing the biological availability of eicosanoids formed by 5-lipoxygenase. (google.es)
  • hydroxy
  • In this terminology S refers to the absolute configuration of the chirality of the hydroxy functional group at carbon position 15. (wikipedia.org)
  • isomers
  • The two isomers, either directly or after being further metabolized, have been suggested to be involved in a variety of human physiological and pathological reactions. (wikipedia.org)
  • proinflammatory
  • The compounds we have identified show promise: they protect beta cell lines and human islets from apoptosis and preserve insulin secretion when challenged by proinflammatory cytokine mixture. (paperity.org)
  • neutrophils
  • In 1975, the first biological activity was attached to this metabolite in studies showing that it simulated the chemotaxis of human neutrophils. (wikipedia.org)