• Resistant Plasmid ducing K. pneumoniae isolates are identifi ed. (cdc.gov)
  • The KPC-3 plasmid was cystitis developed, and the patient underwent cholecys- identical in isolates of both species. (cdc.gov)
  • MDR plasmid transfer from Salmonella to commensal Escherichia coli was demonstrated by PCR and whole-genome sequencing of isolates purified from agar plates containing cefotaxime. (nih.gov)
  • While poultry constitutes the major industry in Nepal, there is a paucity of evidence on colistin resistance in Escherichia coli isolates causing natural infections in poultry. (tropmedres.ac)
  • 109/202) of E. coli isolates was obtained in the liver samples from poultry birds (of both types) aged less than forty days. (tropmedres.ac)
  • In AST, 95.1% (137/144) and 82.6% (119/144) of E. coli isolates were resistant against tetracycline and ciprofloxacin, respectively, while 13.2% (19/144) and 25.7% (37/144) isolates were resistant to cefoxitin and imipenem, respectively. (tropmedres.ac)
  • In the same assay, 76.4% (110/144) E. coli isolates were multi-drug resistant (MDR). (tropmedres.ac)
  • In the PCR assay, 43.9% (18/41) of colistin-resistant isolates were screened positive for plasmid-mediated mcr-1. (tropmedres.ac)
  • Conclusion: The high prevalence of mcr-1 in colistin-resistant E. coli isolates in our study is a cause of concern for the probable coming emergence of colistin resistance in human pathogens, due to horizontal transfer of resistant genes from poultry to human isolates. (tropmedres.ac)
  • In this study, we determined the fitness effects of the major antibiotic resistance plasmid pOXA-48_K8 in wild-type, ecologically compatible enterobacterial isolates from the human gut microbiota. (nature.com)
  • The method was tested with 30 multidrug-resistant clinical isolates including Escherichia coli, Klebsiella pneumoniae and Salmonella enterica subsp. (sorbonne-universite.fr)
  • This study aimed to compare ESBL-producing Escherichia coli causing infections in humans with infecting or commensal isolates from animals and isolates from food of animal origin in terms of the strain types, the ESBL gene present and the plasmids that carry the respective ESBL genes. (reading.ac.uk)
  • A collection of 353 ESBL-positive E. coli isolates from the UK, the Netherlands and Germany were studied by MLST and ESBL genes were identified. (reading.ac.uk)
  • IncI1-Iγ plasmids that carried the blaCTX-M-1 gene were widely disseminated amongst STs in isolates from animals and humans, whereas other plasmids and STs appeared to be more restricted to isolates from specific hosts. (reading.ac.uk)
  • To determine C TX-M types, E. coli isolates were collected from retail chickens (n = 390) and environmental samples from chicken farms (n = 32) and slaughterhouses (n = 67) in Korea. (korea.ac.kr)
  • Fifteen strains harboring bla CTX-M genes were isolated from 358 E. coli isolates. (korea.ac.kr)
  • Our findings demonstrate that the detection of CTX-M types in E. coli isolates is important for tracking ESBL production in animals, and suggest linkage of multiple addiction systems in plasmids bearing bla CTX-M genes. (korea.ac.kr)
  • WGS revealed that all three isolates were E. coli ST410. (bvsalud.org)
  • The aims of this research work were to determine the patterns of antibiotic resistance in Escherichia coli isolates from the meat of wild or domestically reared pigeons from Spain, to detect the presence of virulence and antibiotic resistance genes, and to carry out a phylogenetic classification of the isolates. (mdpi.com)
  • ABSTRACT We investigated antimicrobial resistance patterns and plasmid profiles of uropathogenic Escher- ichia coli isolates from inpatients and outpatients at Jordan University Hospital in 2000 and 2001. (who.int)
  • E. coli accounted for 32.4% and 37.4% of all isolates respectively. (who.int)
  • A large, transferable R-plasmid of 28 kb was found in most E. coli isolates (67%) that were resistant to at least ampicillin, cotrimoxazole and tetracycline. (who.int)
  • Eight to 10 colonies of tance to fluoroquinolones among uropatho- each pure culture of E. coli were taken and genic E. coli isolates [ 1-14 ]. (who.int)
  • We included 53 multidrug-resistant E. munity and hospitalized patients at a major coli isolates that were susceptible to nalid- teaching hospital in Jordan. (who.int)
  • In this study, we molecularly characterized 12 NDM-1 producing clinical Enterobacteriaceae ( Klebsiella pneumoniae , Escherichia coli , Enterobacter cloacae ) isolates that were part of a collection of non-carbapenem susceptible isolates obtained during a one-year period. (who.int)
  • The isolates appear clonally unrelated with MLST, revealing a diversity of clonal types among the Klebsiella pneumoniae and Escherichia coli isolates. (who.int)
  • The first carbapenem-resistant NDM-1 isolates characterized in 2009 were Klebsiella pneumoniae and Escherichia coli isolated from a Swedish patient who had sought medical care in New Delhi, India. (who.int)
  • The 52 isolates comprised the following species: 31 Klebsiella pneumoniae , 13 Escherichia coli , seven Enterobacter cloacae and one Enterobacter aerogenes . (who.int)
  • Infection with carbapenem-resistant K. pneumoniae is as- genes, plasmid isolation, transformation, and Southern sociated with an increased proportion of deaths compared analysis) were performed as described ( 2,4,7 ). (cdc.gov)
  • We used an in vitro chemostat system to approximate the chicken cecal microbiota, simulate colonization by an MDR Salmonella pathogen, and examine the dynamics of transfer of its MDR plasmid harboring several genes, including the extended-spectrum beta-lactamase bla CTX-M1 We also evaluated the impact of cefotaxime administration on plasmid transfer and microbial diversity. (nih.gov)
  • Some of the most clinically relevant resistance genes, such those encoding carbapenemases (ß-lactamase enzymes able to degrade carbapenem antibiotics), are carried on conjugative plasmids that spread across high-risk bacterial clones 5 , 6 . (nature.com)
  • blaOXA-163 was located on a 71-kb IncN plasmid with other resistance genes. (ox.ac.uk)
  • The presence of blaOXA-48-like genes on epidemic plasmids in ST131 is of concern. (ox.ac.uk)
  • The dissemination of antimicrobial resistance genes in Enterobacteriaceae has been largely attributed to plasmids, circular DNA molecules capable of autonomous replication. (sorbonne-universite.fr)
  • ESBL genes were present in a wide variety of E. coli STs. (reading.ac.uk)
  • Conjugations confirmed the transferability of the plasmids carrying blaCTX-M genes to the recipient E. coli J53 strain. (korea.ac.kr)
  • The clonal relationship between the two strains carrying bla CTX-M genes indicates that E. coli may transmit from the farm to retail chickens, suggesting a possible public health risk. (korea.ac.kr)
  • In this work, we have studied the transcription of the virulence regulatory genes virB, virF, and hns (virR) in wild-type enteroinvasive E, coli HN280 and in its pINV-integrated derivative HN280/32. (uniroma3.it)
  • Plasmid delivery through conjugation between bacterial species has proven effective in creating novel antimicrobial agents, targeting specific genes to eliminate or suppress pathogens. (eurekalert.org)
  • The team first optimized this plasmid for bacteria-to-yeast conjugation by deleting 55 genes or small genetic regions to create four streamlined plasmids: M1-M4, with two clones each. (eurekalert.org)
  • Two nucleotides were different between the E. coli (Tn3) and H. ducreyi (pCb) genes that affected the amino-acid sequence. (lu.se)
  • Polymerase chain reaction (PCR) assays and sequencing was used to determine the presence of β-lactamase encoding genes (bla) including bla NDM-1 and plasmid-mediated quinolone and aminoglycoside resistance determinants. (who.int)
  • Transfer occurred to seven E. coli sequence types at high rates, even in the absence of cefotaxime, with resistant strains isolated within 3 days. (nih.gov)
  • Colistin resistance was determined by agar dilution method and colistin-resistant strains were further screened for plasmid-mediated mcr-1 gene, using conventional polymerase chain reaction (PCR). (tropmedres.ac)
  • It was found that the colicinogenic plasmid ColVIK-94 sensitised the strains to hydrophobic antibiotics as well as to some of the hydrophilic and aminoglycoside antibiotics whilst Col BK-98 sensitised the strains to rifampicin and some of the aminoglycosides and hydrophilic antibiotics but not to most of the hydrophobic agents. (ucl.ac.uk)
  • The Col V and Col B plasmids sensitised the strains 1829 and P678-54 to copper sulphate and to the effects of the Tris-EDTA. (ucl.ac.uk)
  • pYD786-1 belongs to the APEC-O1-R-type IncHI2 plasmids, which are widely distributed in human, poultry, and livestock strains. (ox.ac.uk)
  • We have previously shown that integration of the virulence plasmid pINV into the chromosome of enteroinvasive Escherichia coli and of Shigella flexneri makes these strains noninvasive (C. Zagaglia, M. Casalino, B. Colonna, C. Conti, A. Calconi, and M. Nicoletti, Infect. (uniroma3.it)
  • The effect of the introduction of plasmids carrying virB (pBN1) or virF (pHW745 and pMYSW6504), and of a Delta hns deletion, in the different strains was evaluated by measuring beta-galactosidase activity, virB transcription, and virB-regulated virulence phenotypes like synthesis of Ipa proteins, contact-mediated hemolysis, and capacity to invade HeLa cells. (uniroma3.it)
  • At 30 degrees C, the introduction of the high-copy-number plasmid pMYSH6504 (but not of the low copy-number pHW745) or of the Delta hns deletion induces, in strains harboring an autonomously replicating pINV, P-galactosidase activity, virB transcription, and expression of the virulence phenotypes, indicating that, as for HN280/32, the increase in virF gene dosage overcomes the negative regulatory effect of H-NS on virB transcription. (uniroma3.it)
  • E. coli strains bearing the dnaN159 allele display temperature-sensitive growth ( 14 , 35 , 38 ) and altered DNA polymerase (Pol) usage ( 29 , 38 - 40 ). (asm.org)
  • As a result of the impaired β159-Pol III interaction, E. coli strains bearing the dnaN159 allele display increased utilization of the three SOS-regulated DNA polymerases, Pol II ( polB ), Pol IV ( dinB ), and Pol V ( umuDC ) ( 29 , 38 - 40 ). (asm.org)
  • E. coli ) is low to date and the single-dose regimen is well tolerated and has not been associated with selection of drug-resistant bacteria or colonization/infection with multidrug-resistant strains. (drugs.com)
  • Sometimes particular E. coli strains are sub-optimal for plasmid extraction. (zymoresearch.com)
  • When possible, stick to tried and tested strains like E. coli DH5α which contain mutations to lack certain endonucleases and increase plasmid stability. (zymoresearch.com)
  • Thus, these modified E. coli strains are used as workhorses for molecular cloning and plasmid production. (zymoresearch.com)
  • The second method uses chloramphenicol, an antibiotic that halts protein synthesis and decouples it from plasmid replication, when culturing strains containing a plasmid with a relaxed origin of replication. (zymoresearch.com)
  • This R-plasmid reservoir may contribute to the spread of multiple antibiotic resistance in our Region. (who.int)
  • Jo, SJ & Woo, GJ 2015, ' Molecular characterization of plasmids encoding CTX-M β-Lactamases and their associated addiction systems circulating among escherichia coli from retail chickens, chicken farms, and slaughterhouses in Korea ', Journal of microbiology and biotechnology , vol. 26, no. 2, pp. 270-276. (korea.ac.kr)
  • It can also facilitate the conjugative transfer of multidrug resistance (MDR) plasmids between commensal and pathogenic bacteria which is a significant public and animal health concern as it may affect our ability to treat bacterial infections. (nih.gov)
  • Transfer of antimicrobial resistance via plasmid exchange is of particular concern as it enables unrelated bacteria to acquire resistance. (nih.gov)
  • The gastrointestinal tract is replete with bacteria and provides an environment for plasmid transfer between commensals and pathogens. (nih.gov)
  • To further analyze the capacity of mononuclear leukocytes to degrade E. coli DNA, chromosomal and plasmid DNA was isolated from ingested bacteria and subjected to agarose gel-electrophoresis. (jci.org)
  • Plasmids are extra-chromosomal mobile genetic elements able to transfer between bacteria through conjugation 1 . (nature.com)
  • The recent discovery of a plasmid-borne colistin resistance gene, mcr-1, heralds the emergence of truly pan-drug resistant bacteria. (gsdermatology.com.au)
  • In response to this unmet requirement, a team of researchers from Canada have developed and optimized the transfer of superior conjugative plasmids between bacteria and different yeast species via conjugation. (eurekalert.org)
  • These modified plasmids were then transferred from Escherichia coli ( E. coli ) bacteria to S. cerevisiae via conjugation and assessed based on the yeast colony formation. (eurekalert.org)
  • As compared to the original pTA-Mob 2.0 plasmid, the bacteria-yeast conjugation frequency for pSC5 was 10- and 23-fold more when tested in cis (which mobilizes itself) and trans (which mobilizes another plasmid) setups, respectively. (eurekalert.org)
  • This could be because the bacteria had fewer adverse effects on the yeast when it was carrying the pSC5 plasmid. (eurekalert.org)
  • The drug substance anakinra is a recombinant protein (Interleukin-1 receptor antagonist) produced in E. coli bacteria containing an expression plasmid in which a synthetic gene coding for human IL-1Ra has been inserted.According to the Guideline on the Environmental Risk Assessment of Medicinal Products for Human Use (EMEA 2006), proteins are exempted from environmental risk assessment because they are unlikely to result in significant risk to the environment. (janusinfo.se)
  • Plasmid-encoded AmpC expression is also constitutive and may be spread among bacteria usually lacking this beta-lactamase, such as Escherichia coli , Klebsiella pneumoniae , and Proteus mirabilis . (msdmanuals.com)
  • A few months ago, a group of Chinese scientists found that many gram-negative bacteria ( Enterobacteriaceae ) harbor the plasmid gene mcr-1 . (medscape.com)
  • [ 2 ] A plasmid enables bacteria to spread easily to other bacteria and generate resistance to colistin. (medscape.com)
  • [ 4 ] So although just reported, colistin resistance has been developing for some time, perhaps silently, because such bacteria as E coli are rarely tested for colistin resistance. (medscape.com)
  • However, it is important because, having plasmid as a basis, these bacteria can spread to a whole host of organisms. (medscape.com)
  • Here we use the chicken gut microbiota as an exemplar to model the effects of bacterial infection, antibiotic administration, and plasmid transfer. (nih.gov)
  • Bacterial cancer therapy was developed using probiotic Escherichia coli Nissle 1917 (EcN) for medical intervention of colorectal cancer. (nature.com)
  • Plasmid persistence in bacterial populations is strongly influenced by the fitness effects associated with plasmid carriage. (nature.com)
  • However, plasmid fitness effects in wild-type bacterial hosts remain largely unexplored. (nature.com)
  • Moreover, genomic results showed a link between pOXA-48_K8 fitness effects and bacterial phylogeny, helping to explain plasmid epidemiology. (nature.com)
  • Incorporating our fitness results into a simple population dynamics model revealed a new set of conditions for plasmid stability in bacterial communities, with plasmid persistence increasing with bacterial diversity and becoming less dependent on conjugation. (nature.com)
  • These fitness costs make it difficult to explain how plasmids are maintained in bacterial populations over the long-term in the absence of selection for plasmid-encoded traits, a puzzle known as "the plasmid-paradox" 10 . (nature.com)
  • These examples do not necessarily replicate plasmid fitness effects in natural bacterial hosts, which remain largely unexplored. (nature.com)
  • To fully understand plasmid persistence in natural bacterial populations, it will be necessary to address these limitations. (nature.com)
  • In this study, we provide a detailed characterisation of the distribution of plasmid fitness effects in wild-type bacterial hosts. (nature.com)
  • This plasmid contains a constitutive bacterial promoter that does not require induction. (sigmaaldrich.com)
  • We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request. (sigmaaldrich.com)
  • To create our novel plasmids, we built derivatives of the conjugative plasmid, pTA-Mob 2.0, using designed gene deletions and cluster mutations to improve bacterial conjugation with yeasts ," explained Dr. Bogumil J. Karas, Assistant Professor at University of Western Ontario and corresponding author of the study that was published in Volume 2022 of BioDesign Research on 1 September 2022 . (eurekalert.org)
  • to Escherichia coli The Study in Patient In April 2008 a carbapenem-nonsusceptible E. coli strain, marked as Eco2, was recovered from the gall blad- der drainage of a 91-year-old man with dialysis-dependent end-stage renal disease, congestive heart failure, anemia, Moran G. Goren, Yehuda Carmeli, and peptic ulcer disease. (cdc.gov)
  • During his hospital stay, the patient underwent contained a carbapenem-susceptible E. coli strain isogenic screening for carriage of carbapenem-resistant Enter- with the KPC-3-producing isolate, which suggests horizon- obacteriacae (CRE) as part of a routine infection control tal interspecies plasmid transfer. (cdc.gov)
  • Qualified cell banks should be used and tested for identity, viability, strain, genotype/phenotype, and presence/copy number of the plasmid vector of interest, along with a description of its structural elements. (bioprocessintl.com)
  • A prior growth temperature of 25° C seemed to reduce the sensitisation effects to hydrophobic antibiotics of the ColV plasmids without affecting the sensitivity of the parent strain 1829 whereas the effects of the hydrophilic and aminoglycoside e antibiotics were unaffected by a 25° C prior growth temperature. (ucl.ac.uk)
  • An E. coli strain that carries the cloned AsiSI gene from Arthrobacter species (S.K. Degtyarev). (neb.com)
  • In this study, a bla CTX-M-2 -harboring plasmid (pYD786-1) belonging to the sequence type 2 (ST2)-IncHI2 plasmid type and isolated from an Escherichia coli ST410 clinical strain was sequenced and analyzed. (ox.ac.uk)
  • In2 precursor, likely played a role in the generation of the MRR in pN13-01290_23, the putative progenitor plasmid of pYD786-1, found in a foodborne Salmonella strain. (ox.ac.uk)
  • In the current study, the strain E. coli JM109 harboring plasmid p582 was shown to stably express and secrete recombinant beta-glucanase in continuous culture using a minimal medium. (uni-bielefeld.de)
  • The leuB gene, crucial in the leucine biosynthetic pathway, was cloned onto plasmid p582 and the new construct transformed into an E. coli Keio (Delta leuB) knockout strain. (uni-bielefeld.de)
  • The ceftazidime /avibactam-resistant strain uniquely acquired a novel CMY ß-lactamase gene , herein called blaCMY-185, harboured on an IncI-γ/K1 conjugative plasmid . (bvsalud.org)
  • As part of global surveillance and sequencing of carbapenem-resistant Escherichia coli , we identified a sequence type 131 strain harboring bla IMP-14 within a class 1 integron, itself nested within an ∼54-kb multidrug resistance region on an epidemic IncA/C 2 plasmid. (ox.ac.uk)
  • If you are experiencing low yields for your plasmid prep, double check that the strain you're using is best for plasmid propagation. (zymoresearch.com)
  • While many guides will recommend 12-16 hour cultures, every E. coli strain is slightly different in its growth rate and final density. (zymoresearch.com)
  • The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)
  • The patient was treated with ertapenem, ducing Escherichia coli was isolated from a carrier of KPC- metronidazole, colistin, and vancomycin. (cdc.gov)
  • Detection of Plasmid-Mediated Colistin Resistant mcr-1 Gene in Escherichia coli Isolated from Infected Chicken Livers in Nepal. (tropmedres.ac)
  • Background: Plasmid-mediated resistance to the colistin in poultry is considered as an emerging problem worldwide. (tropmedres.ac)
  • This study aimed to explore the prevalence of plasmid-mediated colistin resistance gene, mcr-1 in E. coli isolated from liver samples of dead poultry suspected of E. coli infections. (tropmedres.ac)
  • What was believed to be a routine Escherichia coli urinary tract infection harbored a particular gene making it resistant to colistin , usually viewed as a last-resort antibiotic for the treatment of gram-negative infections. (medscape.com)
  • Being plasmid-mediated is a concern especially as colistin use is the "canary in the coal mine" of gram-negative resistance. (medscape.com)
  • The binding of SeqA protein to hemimethylated GATC sequences is important in the negative modulation of chromosomal initiation at oriC, and in the formation of SeqA foci necessary for Escherichia coli chromosome segregation. (wikipedia.org)
  • On the Escherichia coli chromosome the three substitution mutations acted similarly to a recF deletion in reducing UV resistance in a recB21 recC22 sbcB15 sbcC201 genetic background. (umass.edu)
  • AmpC beta-lactamase may be chromosome- or plasmid-encoded. (msdmanuals.com)
  • Chemostat culture has also been used to examine the possibility of plasmid transfer by transformation within the chemostat. (warwick.ac.uk)
  • The results obtained are discussed in relation to previously published transformation work using batch grown cells and a possible mechanism of plasmid transformation is suggested. (warwick.ac.uk)
  • Chemically competent E. coli cells suitable for high efficiency transformation and protein expression, featuring control of IPTG induced expression with non-T7 plasmids. (neb.com)
  • Escherichia coli JM109 and plasmid pTZ57R/T (MBI Fermentas, Hanover, Maryland, USA) were used as transformation host and cloning vector, respectively. (scialert.net)
  • The plasmid pSP65 which has cloned the complete HBV sequence, was amplified by transformation of coli (DH5). (bvsalud.org)
  • Restriction maps of the recombinant plasmids, termed pTf100 and pTf110, respectively, were constructed, creating potential cloning vehicles for exchanging genetic information between E. coli and T. ferrooxidans. (unab.cl)
  • The secretion of recombinant proteins into the extracellular space by Escherichia coli presents advantages like easier purification and protection from proteolytic degradation. (uni-bielefeld.de)
  • Significantly, it showed a stable maintenance of the recombinant plasmid in the absence of any antibiotics. (uni-bielefeld.de)
  • The plasmid stability in a continuously cultivated E. coli fermentation, in the absence of antibiotics, with extracellular secretion of recombinant protein provides an interesting model for further improvements. (uni-bielefeld.de)
  • R.S.V. Selvamani, K. Friehs, and E. Flaschel, "Extracellular recombinant protein production under continuous culture conditions with Escherichia coli using an alternative plasmid selection mechanism", Bioprocess and Biosystems Engineering , vol. 37, 2014, pp. 401-413. (uni-bielefeld.de)
  • PCR-based replicon typing is currently the standard method for plasmid identification but there are new classification schemes, especially the relaxase gene typing (PRaseT). (sorbonne-universite.fr)
  • One method for plasmid amplification uses an inhibitory amount of chloramphenicol (170 µg/ml) added to a culture, which is then incubated further until plasmid purification (typically the next day) 2 . (zymoresearch.com)
  • Microbiologic and molecular investigations (pulsed- by a plasmid-encoded KPC, which confers resistance to fi eld gel electrophoresis [PFGE], DNA isolation, isoelec- all cephalosporins, monobactams, and carbapenems ( 4 ). (cdc.gov)
  • Subsequently, the pSC5 plasmid was domesticated for Golden Gate Assembly-a molecular cloning method enabling the simultaneous and directional assembly of multiple desirable DNA fragments into a single piece. (eurekalert.org)
  • Characterization of ESBL gene-carrying plasmids was performed using PCR-based replicon typing. (reading.ac.uk)
  • Only nominal studies in characterization of choline dehydrogenase from E . coli have been reported to date. (scialert.net)
  • In this study, we report the characterization and structural analysis of choline dehydrogenase in E . coli . (scialert.net)
  • Using these conditions the segregational stability of two related multicopy plasmids (pDSII09 and pBR322) was examined. (warwick.ac.uk)
  • The functional basis of the differential segregation exhibited by these plasmids was ascribed to the presence (on pDSII09) or absence (on pBR322) of a functional par (partition) signal that ensured the efficient segregation of plasmid molecules into daughter cells at division. (warwick.ac.uk)
  • The 6.7-kilobase plasmid (pTf1) was cloned separately into the HindIII or BamHI site of Escherichia coli plasmid pBR322. (unab.cl)
  • We show that transfer of a multidrug-resistant plasmid from the zoonotic pathogen Salmonella to commensal Escherichia coli occurs at a high rate, even in the absence of antibiotic administration. (nih.gov)
  • The effects of Col plasmids on sensitivities of E.coli K-12 derivatives, especially 1829 and P678-54" to hydrophobic, hydrophilic and aminoglycoside antibiotics and to other inhibitors such as copper ions and Tris-EDTA have been examined. (ucl.ac.uk)
  • To avoid the use of antibiotics and render this process more sustainable, alternative plasmid selection mechanisms were required. (uni-bielefeld.de)
  • There are a couple ways to ensure high yield plasmid preparations from E. coli cultures by using antibiotics for selective pressure. (zymoresearch.com)
  • ABSTRACT The extended-spectrum β-lactamase gene bla CTX-M-2 is mainly associated with IS CR1 embedded in complex sul1 -type integrons, but information on the genetic context of plasmids harboring the IS CR1-bla CTX-M-2 module remains limited. (ox.ac.uk)
  • Plasmid M3 clone 1 (M3C1) showed the most significant increase in conjugation efficiency. (eurekalert.org)
  • The segregational instability of the plasmid in the absence of antibiotic selection pressure was demonstrated. (uni-bielefeld.de)
  • One hundred samples from healthy animals were screened for the presence of enterohaemorrhagic Escherichia coli 0157: H7 and 17 were positive for EHEC 0157:H7 after confirmation using serology kits. (edu.ng)
  • High efficiency chemically competent E. coli cells suitable for non T7 expression with enhanced reduction of basal expression. (neb.com)
  • To show this, we altered a low-level overexpressing recF+ plasmid with a set of structurally neutral mutations that increased the rate of expression of recF. (umass.edu)
  • The technique of continuous culture has been used to study the segregatlonal stability of plasmids in Escherichia coli in the absence of selective pressure. (warwick.ac.uk)
  • The first using a previously characterised par function from plasmid pSCIOI and the second using a fragment of plasmid pDSII09 that was considered (by argument) to be the region involved in its observed segregational stability. (warwick.ac.uk)
  • In E . coli , the biosynthetic pathway for the production of glycine betaine from choline has been well characterized at the genetic level ( Landfald and Strom, 1986 ). (scialert.net)
  • Furthermore, the pSC5 plasmid allowed for successful DNA transfer to seven yeast species, including Candida auris -a known pathogen-albeit with varying levels of efficiency. (eurekalert.org)
  • Plasmids used as therapeutic agents tend to be relatively small in size (4.5- 6.0 kb and even down to 2.5 kb) ( 1 ), requiring relatively low quantities to support early phase clinical trials (1-5 g of purified pDNA). (bioprocessintl.com)
  • Cobra Biologics has been producing plasmid DNA for a number of clinical applications for over 15 years at an approved site in the United Kingdom under the European Union (EU) clinical trials directive. (bioprocessintl.com)
  • Generated plasmids have been used in clinical trials for gene therapy and vaccines as well as in viral vector production. (bioprocessintl.com)
  • Plasmid partition relies on three structures, i.e. a centromere like DNA site, a centromere-binding protein and an ATPase or a GTPase motor protein for plasmid positioning. (sorbonne-universite.fr)
  • This plasmid contains an Outer Membrane Protein T (OmpT) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. (sigmaaldrich.com)
  • Chloramphenicol treatment can stop protein production but allow the E. coli to continue to "amplify" the plasmids, resulting in increased yields during plasmid purification 1 . (zymoresearch.com)
  • To characterize a blaCMY variant associated with ceftazidime /avibactam resistance from a serially collected Escherichia coli isolate. (bvsalud.org)
  • Incubate cultures at 37°C. Isolate DNA using standard plasmid preparation procedures. (atcc.org)
  • These results help to explain the high prevalence of plasmids in the greatly diverse natural microbial communities. (nature.com)
  • Healthy cattle are a reservoir of E. coli O157:H7 and bovine food products and fresh produce contaminated with bovine waste are the most common sources for disease outbreaks in the United States. (jmb.or.kr)
  • Three major virulence factors of E. coli O157:H7 have been identified including Shiga toxins, a pathogenicity island called the locus of enterocyte effacement, and an F-like plasmid, pO157. (jmb.or.kr)
  • blaOXA-48 was present on IncL/M plasmids, genetically similar to other blaOXA-48 plasmid sequences, and consistent with interspecies/interlineage spread. (ox.ac.uk)
  • By contrast, the insertion mutation on the high-level overexpressing plasmid showed no partial activity and can be considered a true null mutation. (umass.edu)
  • Thereafter, five derivative plasmids of M3C1 were created containing the traJ mutation, including the pSuperCon5 (pSC5) plasmid with additional elements for improved conjugative transfer to diverse yeast species and diatoms. (eurekalert.org)
  • It was shown that both mutant plasmids sensitise 1829 to these two agents. (ucl.ac.uk)
  • Use of high and low level overexpression plasmids to test mutant alleles of the recF gene of Escherichia coli K-12 for partial activity. (umass.edu)
  • The Escherichia coli dnaN159 allele encodes a mutant form of the β-sliding clamp (β159) that is impaired for interaction with the replicative DNA polymerase (Pol), Pol III. (asm.org)
  • However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)
  • The slight difference in electrophoretic mobility of normal Fp and that produced in E. coli could be due to the absence of covalently FAD but could have another explanation because this difference was not observed for some mutant Fp without covalently bound FAD [3]. (lu.se)
  • Plasmid DNA of E. coli carrying a gene coding for ampicillin resistance remained intact for a 2-h period after ingestion, and was still able to transform recipient E. coli cells after this period. (jci.org)
  • This method, called "plasmid partition gene typing" (PAR-T), was validated with 136 transconjugants or transformants harboring various replicon types. (sorbonne-universite.fr)
  • The most common plasmid replicon type overall was IncI1-Iγ followed by multiple IncF replicons. (reading.ac.uk)
  • Whether a vector is kept at low copy number, low culture density, or culture overgrowth, this guide will help you navigate your purification woes and determine the best way to boost your plasmid yields from E. coli cultures. (zymoresearch.com)
  • RESUME Nous avons étudié la résistance aux antimicrobiens et les profils plasmidiques des isolats d' Escherichia coli uropathogénique chez des malades hospitalisés et des malades externes à l'Hôpital universitaire jordanien en 2000 et 2001. (who.int)
  • However, when pDNA is used as a transfection material, multiple plasmid constructs often are required to produce an individual product coupled with an escalating demand for plasmid quantity as production scales increase. (bioprocessintl.com)
  • Sometimes, plasmid purification just doesn't go according to plan. (zymoresearch.com)
  • The efficacy of column-based plasmid purification doesn't just depend on how much plasmid is loaded onto the column, but also the total amount of biomass being loaded as well. (zymoresearch.com)
  • If you want to scale up your plasmid purification, try using a kit designed for high inputs of culture, such the ZymoPURE II Midipreps and Maxipreps , which can process more culture. (zymoresearch.com)
  • Purification from cultures grown in highly enriched media such as Terrific Broth (TB) yield higher amounts of plasmid DNA per ml of culture than standard Luria-Bertani Broth (LB). (zymoresearch.com)
  • A variation of this method that reports higher plasmid yield uses lower amounts of chloramphenicol (10-20 µg/ml) added to exponentially growing cells that are subsequently incubated overnight prior to plasmid purification 3 . (zymoresearch.com)
  • A pCb plasmid encoding a beta-lactamase from Haemophilus ducreyi was transferred to Escherichia coli, purified, and characterized. (lu.se)
  • OXA beta-lactamases primarily hydrolyze narrow spectrum penicillins but certain OXA variants such as the plasmid encoded OXA 48 can hydrolyze carbapenems, even while leaving many cephalosporins active. (msdmanuals.com)
  • The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. (sigmaaldrich.com)
  • The bla NDM-1 gene was encoded on plasmids that were easily transferable. (who.int)
  • The ESBL-producing E. coli represented 158 different STs with ST131, ST10 and ST88 being the most common. (reading.ac.uk)
  • Treatment of uncomplicated UTIs (acute cystitis) in women caused by susceptible Escherichia coli or Enterococcus faecalis (formerly Streptococcus faecalis ). (drugs.com)
  • pTA-Mob 2.0 is composed of genetic elements required for plasmid maintenance and transfer, making it ideal for this study. (eurekalert.org)