Homologous 1998 analysis tandem repeat sequences. Medical search. Frequent questions

Genes are not randomly distributed on a chromosome as they were thought even after removal of tandem repeats. (biomedcentral.com)
In order to further investigate the mode of tissue-specific gene clustering in higher eukaryotes, we have performed a genome-scale analysis of positional clustering of the mouse testis-specific genes. (biomedcentral.com)
Our computational analysis shows that a large proportion of testis-specific genes are clustered in groups of 2 to 5 genes in the mouse genome. (biomedcentral.com)
Eukaryotic genes appear to be transcribed individually and are thought to be scattered on chromosomes without apparent organization by function or positional expression except for tandem duplication. (biomedcentral.com)
9 ] performed a microarray analysis of genes expressed in the human adipose tissue. (biomedcentral.com)
Using EST ( Expressed Sequence Tag ), they showed positional clustering of these genes. (biomedcentral.com)
CENP-Bs also repress solo long terminal repeats (LTRs) and LTR-associated genes. (nature.com)
However, these analyses provided weak supportive results because they were based on only one of several genes. (frontiersin.org)
Genes are part of the DNA sequence called coding DNA. (encyclopedia.com)
Noncoding DNA represents sequences that do not have genes and only recently have been found to have many new important functions. (encyclopedia.com)
Out of the 3 billion base pairs that exist in human DNA, the sequence obtained from the Human Genome Project has revealed that there are only about 30, 000 genes. (encyclopedia.com)
The complex polyploid nature of the genome of bread wheat, Triticum aestivum , and the technically difficult task of sequencing clusters of tandem repeats mean that the detailed organization of extended genomic regions containing 5S rRNA genes remains unclear. (biomedcentral.com)
To address this challenge, we developed a new assembly strategy and improved the de novo genome assembly, producing a 1.56 Gb genome sequence (DjGenome ver1.0, including 202,925 scaffolds and N50 length 27,741 bp) that covers 99.4% of all 19,543 genes in the assembled transcriptome, although the genome is fragmented as 80% of the genome consists of repeated sequences (genomic frequency ≥ 2). (biomedcentral.com)
In order to estimate how many different genes might be responsible for hearing loss in this population, we evaluated ten families for linkage to all 36 known human autosomal deafness-related genes, fully sequencing hearing-related genes at any linked sites in informative relatives. (biomedcentral.com)
Systematic population-wide genomic analysis may be the most effective way to identify all critical genes and alleles for heterogeneous phenotypes in communities with many small isolates. (biomedcentral.com)
SpiroESTdb provides an integrated platform for expressed sequence data, expression dynamics, functional genes, genetic markers including single nucleotide polymorphisms and tandem repeats, gene ontology and KEGG pathway information. (biomedcentral.com)
Analysis of sequenced genomes revealed that transcription factors compose up to 10% of metazoan genomes [ 2 ] and more than 5% of the genes in Arabidopsis were identified as transcription factors [ 3 ]. (biomedcentral.com)
In diploid cells, homologous chromosomes occupy separate territories, but expression from genes located on either the paternal or maternal homolog is usually similar. (biomedcentral.com)
Yeast one-hybrid assays showed that SsDREB protein specifically binds to the DRE sequence and could activate the expression of reporter genes in yeast, suggesting that the SsDREB protein was a CBF/DREB transcription factor. (hindawi.com)
Numerous abiotic stress-related genes and transcription factors (TFs) have been isolated from different plant species and overexpressed in homologous and heterologous systems to engineer stress tolerance [ 5 ]. (hindawi.com)
The A-1 subgroup, which includes the DREB1/CBF- (C-repeat binding factor-) like genes, are mainly induced by low temperature and activate the expression of many cold stress-responsive genes, whereas the A-2 subgroup, which is comprised of the DREB2 genes, mainly functions in osmotic stress [ 7 ]. (hindawi.com)
DREB2 homologous genes have been isolated from a variety of species [ 12 ]. (hindawi.com)
Korkko J, Ala-Kokko L, De Paepe A, Nuytinck L, Earley J, Prockop DJ: Analysis of the COL1A1 and COL1A2 genes by PCR amplification and scanning by conformation-sensitive gel electrophoresis identifies only COL1A1 mutations in 15 patients with osteogenesis imperfecta type I: identification of common sequences of null-allele mutations. (smpdb.ca)
However, thorough in-depth identification or systematic analysis of GRAS family genes in foxtail millet has not been conducted. (biomedcentral.com)
Based on the number of conserved domains and gene structure, the SiGRAS genes were divided into 13 subfamilies via phylogenetic tree analysis. (biomedcentral.com)
Genetic structure analysis showed that most SiGRAS genes lacked introns. (biomedcentral.com)
Some SiGRAS genes were derived from gene duplication events, and segmental duplications may have contributed more to GRAS gene family expansion than tandem duplications. (biomedcentral.com)
A substantial portion of the regulatory interactions in the higher eukaryotic cell are mediated by simple sequence motifs in the regulatory segments of genes and (pre-)mRNAs, and in the intrinsically disordered regions of proteins. (biomedcentral.com)

A local high frequency of microhomologies, macrohomologies, tandem repeats, and repeat/repetitive sequences contribute to a high rate of nonallelic homologous recombination involving NEMO , resulting in the development of de novo deletion mutations. (medscape.com)
This kit employs SMART technology for the amplification of full-length cDNAs, in combination with cloning using homologous recombination. (biomedcentral.com)
In the latest version of the method, part of the reporter vector containing the cis -element of interest upstream of the reporter gene is introduced into the yeast genomic DNA via homologous recombination with a non-essential gene [ 9 ]. (biomedcentral.com)
Olds RJ, Lane DA, Chowdhury V, De Stefano V, Leone G, Thein SL: Complete nucleotide sequence of the antithrombin gene: evidence for homologous recombination causing thrombophilia. (smpdb.ca)
Major sequence differences among the four specimens sequenced in this study were in the 5′ and 3′ ends of chromosome 6 and the gp60 region, largely the result of genetic recombination. (cdc.gov)
The sequence similarity among specimens of the two dominant outbreak subtypes and genetic recombination in chromosome 6, especially around the putative virulence determinant gp60 region, suggest that genetic recombination plays a potential role in the emergence of hyper-transmissible C. hominis subtypes. (cdc.gov)

We have determined the complete nucleotide sequence of a 13.5kb EcoRI genomic fragment comprising the only two 3.3kb elements left in the affected D4Z4 locus of a patient with FSHD. (nih.gov)
Complete nucleotide sequence and genome organization of a Drosophila transposable genetic element, 297. (geneticsmr.com)
The former is highly conserved in structure, whereas the NTS is polymorphic in both length and nucleotide sequence. (biomedcentral.com)
D'Alessio M, Bernard M, Pretorius PJ, de Wet W, Ramirez F: Complete nucleotide sequence of the region encompassing the first twenty-five exons of the human pro alpha 1(I) collagen gene (COL1A1) Gene. (smpdb.ca)
These genomes had complete synteny in gene organization and 96.86-97.0% and 99.72-99.83% nucleotide sequence similarities to the published genomes of C. parvum and C. hominis , respectively. (cdc.gov)
databases agreed to adopt the NCBI taxonomy as the standard classification for the nucleotide sequence databases. (nih.gov)

The Drosophila melanogaster gypsy transposable element encodes putative gene products homologous to retroviral proteins. (geneticsmr.com)
A part of the promoter or tandem copies of putative transcription regulatory DNA elements are cloned upstream of a reporter gene in a reporter vector. (biomedcentral.com)

They consider two ORFs to be adjacent if they occur on the same chromosome and if there are no other ORFs or functional elements such as Ty element, long terminal repeats, tRNAs, snRNAs between them. (biomedcentral.com)

The nucleic acid strands are composed of a sequence of nucleotides. (encyclopedia.com)
Advances in cellular and molecular biology experimental methods, as well as nucleic acid sequencing technologies, have helped increase our ability to examine the biology of planarians at the molecular level. (biomedcentral.com)
The methods of the invention relate generally to amplification of nucleic acid on a solid support and analysis of the amplified nucleic acid. (justia.com)

In this study, we sequenced the genomes of two field specimens from each of the two subtypes and conducted a comparative genomic analysis of the obtained sequences with those from the only fully sequenced Cryptosporidium parvum genome. (cdc.gov)

The PUM repeat with the N- and C-terminal regions pack together to form a right-handed superhelix that approximates a half doughnut structurally similar to the Armadillo (ARM) repeat proteins, beta-catenin and karyopherin alpha. (embl-heidelberg.de)
Two previously undetected domains were identified in a variety of RNA-binding proteins, particularly RNA-modifying enzymes, using methods for sequence profile analysis. (embl-heidelberg.de)
Four repeated domains in the Fasciclin I family of proteins, present in many other contexts. (embl.de)
These proteins are usually GPI anchored and consist of: (i) a single FAS1 domain, (ii) a tandem array of FAS1 domains, or (iii) FAS1 domain(s) interspersed with other domains. (embl.de)
These are the arrestin-domain containing proteins and a set of families that are rather distantly related to arrestins with maximal 10% sequence identity [ 8 ]. (biomedcentral.com)
In addition, origin activity can be regulated from sequences such as "locus control regions" that are many kilobases distal to the OBR but affect the accessibility of initiation sites to replication proteins. (nih.gov)
Meanwhile, the C-terminal sequence of GRAS transcription factors is relatively conserved, has specific transcription regulatory domains that bind to DNA or other proteins, and can recognize a variety of different target receptors. (biomedcentral.com)
The high sequence conservation between C. parvum and C. hominis genomes and significant differences in copy numbers of MEDLE family secreted proteins and insulinase-like proteases indicate that telomeric gene duplications could potentially contribute to host expansion in C. parvum . (cdc.gov)
HN - 2017 (1996) MH - ADAMTS Proteins UI - D000071096 MN - D8.811.277.656.675.374.102.500 MN - D9.400.430.500.500 MN - D12.776.395.33.500 MN - D12.776.860.300.85 MS - A subfamily of ADAM proteases that are distinguished by the presence of one or more THROMBOSPONDIN type-1 repeats (TSRs). (nih.gov)
In this analysis, these proteins showed fewer cross-reactions with the tested sera. (biomedcentral.com)
In this study, the proteins of C. burnetii Xinqiao, a phase I strain isolated in China [ 13 ], were analyzed with sera from experimentally infected BALB/c mice and Q fever patients using immunoproteomic analysis. (biomedcentral.com)

660. Chowdhury, M.R., Mathur, R., and Verma, I.C. (1998) Utility of XY-amelogenin gene primers for detection of sex chromosomes. (nist.gov)
The function of chromosomes is to package the extremely long DNA sequence. (encyclopedia.com)

Together with previously reported mitochondrial genome sequences of the pol and nap mitotypes in B. napus [ 6 , 7 ], we compared the six mitochondrial genome sequences to learn the mechanism of formation for Brassica mitochondrial genomes. (biomedcentral.com)

5S rDNA has long been a favored target for cytological and phylogenetic studies due to the inherent peculiarities of its structural organization, such as the tandem arrays of repetitive units and their high interspecific divergence. (biomedcentral.com)
We conclude that sequence analysis of the two repetitive loci introduced here may be highly useful for routine typing of C. difficile . (biomedcentral.com)

An integrated platform for Brucella with knowledge graph technology: From genomic analysis to epidemiological projection. (cdc.gov)

The TIM barrel is formed from a sequence of β-α-β motifs closed by the first and last strand hydrogen bonding together, forming an eight stranded barrel. (wikipedia.org)
These are excellent at detecting repeats and other genomic motifs, but most lack a high quality visualization feature. (biomedcentral.com)
Identification of motifs for cell adhesion within the repeated domains of transforming growth factor-beta-induced gene, betaig-h3. (embl.de)

In non-affected individuals, this locus comprises 10-100 tandem copies of members of the 3.3kb dispersed repeat family. (nih.gov)
Pumilio family members are characterised by the presence of eight tandem copies of an imperfectly repeated 36 amino acids sequence motif, the Pumilio repeat, surrounded by a short N- and C-terminal conserved region. (embl-heidelberg.de)
PCR (polymerase chain reaction) technique has achieved increased importance for post-mortem DNA analysis in forensic cases 8 because of the millions of copies amplified from one specific sequence of DNA. (bvsalud.org)
The targets of interest are then amplified in a defined location so that many copies of the same target sequence are generated in a single location. (justia.com)

betaig-h3 is a transforming growth factor-beta-inducible cell adhesion molecule that has four characteristic homologous repeated domains. (embl.de)
Generally, it is thought that Env epitopes that induce broadly neutralizing Abs are difficult to access and poorly immunogenic because of the characteristic oligomerization, conformational flexibility, sequence diversity and extensive glycosylation of Env protein. (plos.org)

analysis showed 3 major clusters. (cdc.gov)
The number of clusters is much higher than expected by chance even after removal of tandem repeats. (biomedcentral.com)
After amplification, these distinct clusters of DNA are then subject to repeated cycles of ligation or reversible single base chain terminated extension. (justia.com)

758. Fregeau, C.J., Bowen, K.L., and Fourney, R.M. (1999) Validation of highly polymorphic fluoresceht multiplex short tandem repeat systems using two generations of DNA sequencers. (nist.gov)

Moreover, tandem repeat sequences enabled the reconstruction of the isolates' largely clonal population structure and evolutionary history. (biomedcentral.com)
To obtain whole-genome information on this species and to study its gene regulatory networks, we extracted genomic DNA from 200 planarians derived from a laboratory-bred asexual clonal strain, and sequenced 476 Gb of data by second-generation sequencing. (biomedcentral.com)
Methods for solid phase clonal amplification of hybridized target molecules on the surface of the arrays or short oligonucleotides of know sequence and location are disclosed. (justia.com)

MEGA: a biologist-centric software for evolutionary analysis of DNA and protein sequences. (geneticsmr.com)

In total, 5,760 randomly selected clones were picked and sequenced. (biomedcentral.com)

The employed strategy enabled us to map in detail the duplication- and deletion history of arrestin paralogs including tandem duplications, pseudogenizations and the formation of retrogenes. (biomedcentral.com)

61. Clayton, T.M., Whitaker, J.P. and Maguire, C.N. (1995) Identification of bodies from the scene of a mass disaster using DNA amplification of short tandem repeat (STR) loci. (nist.gov)
We describe two tandem repeat loci, designated 'TR6' and 'TR10', which display extensive sequence variation that may be useful for sequence-based strain typing. (biomedcentral.com)
2013), who found that including less informative loci added so much noise to the phylogenetic signal that the analysis eventually broke down. (blogspot.com)

For contemporary functional genomic studies, the significant efforts invested in the creation of integrative transcript databases of expressed sequence tags (ESTs) derived from full-length cDNA libraries have provided opportunities to direct gene discovery and functional analysis. (biomedcentral.com)

HN - 2017(1998) FX - Antigen-Antibody Reactions MH - Acute Febrile Encephalopathy UI - D000071072 MN - C10.228.140.21 MS - Acute onset of fever accompanied by seizures, cerebral inflammation and a change in mental status (e.g., confusion, disorientation, and coma). (nih.gov)

Several other methods have been used to type different lates obtained from different geographic areas or different isolates of the same species, in particular, multilocus hosts or were implicated in different manifestations of enzyme electrophoresis (15) and multilocus sequence typ- human disease caused by C. burnetii . (cdc.gov)

Recent phylogenetic analyses based on the complete chloroplast genome sequence suggested strong support for the position of Vitaceae as the earliest diverging lineage of rosids and placed it as a sister to the remaining rosids. (frontiersin.org)
Whole genome sequence and expressed sequence tags (ESTs) of the eucalypts paved way for introduction of molecular genetics and breeding in this genus. (springeropen.com)

The term motif denotes a repeated figure or design and, in motif biology, the occurrence of a given class of motif in a set of unrelated biomolecules led to the appropriation of the term to refer to a recurrent pattern of nucleotides or amino acids that corresponds to an autonomous functional module. (biomedcentral.com)
The tetratricopeptide-repeat region of KLC1 recognizes the ELEWED sequence (amino acids 115-120) of caytaxin. (biologists.com)

To understand the evolutionary peculiarity of plant mitotypes, which are defined as mitochondrial genome types of which there can be more than one within one plant species or genus, more systematic sequences are needed. (biomedcentral.com)
To date, no systematic sequences of one angiosperm genus with multiple species had been used to analyze the derivation of mitochondrial genome, and thus the mechanism underlying the peculiarity has not been revealed. (biomedcentral.com)
HAYSTAC: A Bayesian framework for robust and rapid species identification in high-throughput sequencing data. (cdc.gov)
The development of whole-genome sequencing has allowed the GRAS gene family to be identified and characterized in many species. (biomedcentral.com)

Each block of three nucleotides, called codons, in the mRNA sequence encodes for a specific amino acid , the building blocks of a protein. (encyclopedia.com)

These two regions differ in the organization of both 5S rDNA and the neighboring sequences comprised of transposable elements, implying different modes of evolution for these regions. (biomedcentral.com)

The methods may also be used for quantitative analysis of expression levels of RNA. (justia.com)
In quantitative PCR analysis, the copy number per mouse was obtained with 1% of the DNA sample extracted from 10 mg spleen tissue. (biomedcentral.com)

Intron/exon gene organization and analysis of the 5'-flanking region. (smpdb.ca)

688. Akane, A. (1998) Sex determination by PCR analysis of the X-Y amelogenenin gene. (nist.gov)
In preferred aspects the analysis is a determination of sequence at one or more locations in the amplified target. (justia.com)
Inheritance, homology by common descent, and the conservation of sequence and structure in the determination of function are all central ideas in biology that are directly related to the evolutionary history of any group of organisms. (nih.gov)

Using synonymous alleles to enlarge sequence space exploration, we have retrieved 13 mutations affecting both I2 and the multimerization domains of IntI1. (elifesciences.org)

Origins in the fission yeast Schizosaccharomyces pombe lack a genetically required consensus sequence, although they do exhibit "autonomously replicating sequence" (ARS) activity. (nih.gov)

A chromosome is a threadlike structure found in the nucleus of most cells that carries the genetic material in the form of a linear sequence of deoxyribonucleic acid (DNA). (encyclopedia.com)
Different molecular characterization approaches have been used simultaneously in eucalypts, however, microsatellites or simple sequence repeats (SSRs) with their prolific characteristics could occupy a special niche in Eucalyptus genetic improvement. (springeropen.com)
This review on eucalypts microsatellites, highlights the availability and characteristics of genomic and eSSRs and their potential in genetic analysis of natural and breeding populations and also discusses the future prospects in population genetics and marker assisted selection. (springeropen.com)
Efforts on molecular breeding and molecular genetic analysis are underway in eucalypts to accelerate breeding and conservation. (springeropen.com)

Mile strain was sequenced (14). (cdc.gov)

Deletions leaving 1-8 such repeats have been associated with FSHD, for which no candidate gene has been identified. (nih.gov)

However, Skittle is also more generally useful for analysis of any genomic data, allowing users to correlate published annotations and observable visual patterns, and allowing for sequence and construct quality control. (biomedcentral.com)
In the present study, we sequenced the complete chloroplast genome of A. brevipedunculata and used these data to assess the relationships among 32 angiosperms, including 18 taxa of rosids. (frontiersin.org)
Burleigh and Mathews (2004) reported that based on rootings of phylogenetic tree analysis of DNA sequences data suggested that angiosperms are sister group to all other seed plants, whereas, Ginkgo and cycads separate angiosperm groups. (frontiersin.org)
Typing procedures based on DNA sequences overcome these limitations, since sequence data may easily be exchanged and stored in databases that are accessible via the internet. (biomedcentral.com)
Comprehensive descriptions of the sparganum content of all sequenced data are available, including summary reports. (biomedcentral.com)
This integrative web-based database of sequence data, functional annotations and expression profiling data will serve as a useful tool to help understand and expand the characterization of parasitic infections. (biomedcentral.com)
Nevertheless, these data fail to reveal the transcriptional regulatory mechanisms because of the complexity of the promoter sequences, the relatively small number of cis -regulatory elements and transcription factors that have been characterised, and lack of data about cooperative regulation of gene expression in plants by several transcription factors simultaneously. (biomedcentral.com)
The funder provided support in the form of salaries for authors [C.S, T.B, J.G., and B.J.D.] and research materials, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. (plos.org)
In all three cases, the relationships were just as complex after the genome sequencing as before - the resolution of controversial branches in our trees did not occur as a result of increased access to character data. (blogspot.com)
Currently, with the application of molecular biology resources in human identification, it became possible to identify a person even without physical ante-mortem data or with deteriorated biological material in negligible amounts, which are actually relatively common conditions in forensic analyses 7,12,16,19 . (bvsalud.org)
However, in recent times, tremendous data on gSSRs and eSSRs are made available through next generation sequencing (NGS) methods (Zalapa et al. (springeropen.com)
Both systems allow searching of the Taxonomy database for names, and both link to the relevant sequence data. (nih.gov)

We analyzed the evolutionary relationships of Brassica mitotypes by sequencing. (biomedcentral.com)
We have sequenced four Brassica mitotypes, compared six Brassica mitotypes and suggested a mechanism for mitochondrial genome formation in Brassica , including evolutionary events such as inheritance, duplication, rearrangement, genome compaction, and mutation. (biomedcentral.com)
Here, we selected Brassica as an evolutionary clade in which to analyze the evolutionary relationships of Brassica mitotypes by sequencing. (biomedcentral.com)

Origins in multicellular organisms generally lack ARS activity, although they do exhibit "replicator" activity (the ability to impart origin activity when translocated to other chromosomal sites), and they can be inactivated by sequence alterations. (nih.gov)

To begin to decipher the molecular processes underlying gene function, we constructed a database of expressed sequence tags (ESTs) generated from sparganum. (biomedcentral.com)
After molecular analysis through PCR technique, it was verified that DNA amplification of the samples was not possible at any of the periods and temperatures analyzed. (bvsalud.org)
As a combined molecular and cytological approach, the major advantage of this visually appealing technique resides in its unique ability to provide an intermediate degree of resolution between DNA analysis and chromosomal investigations while retaining information at the single-cell level. (biosyn.com)

Phylogenetic analysis inferred asymptomatic or appear as atypical pneumonia, granulo- from compiled sequences characterized 3 monophyletic matous hepatitis, or self-limited febrile illness. (cdc.gov)

This nucleotide display is accompanied by a "repeat map" which comprehensively displays all local repeating units, based upon analysis of all possible local alignments. (biomedcentral.com)
This protein was classified in the A-6 group of DREB subfamily based on multiple sequence alignments and phylogenetic characterization. (hindawi.com)

However, the number of molecules hybridized to the probes is generally lower than the amount necessary for efficient application of some next generation sequencing chemistries. (justia.com)
In preferred embodiments an array of probes complementary to a plurality of sequences of interest are hybridized to targets. (justia.com)
Probes and targets are finally visualized in situ by microscopy analysis. (biosyn.com)

Viral origins contain a specific core DNA sequence that is required for initiation to proceed efficiently, whereas the requirement for specific DNA sequences is greatly diminished in single cell eukaryotes and virtually absent in multicellular eukaryotes. (nih.gov)
Thus, origins in multicellular eukaryotes are more similar to those in S. pombe than to those in S. cerevisiae in terms of their size, sequence complexity, and their lack of a required consensus sequence. (nih.gov)

The PUM-HD domain is a sequence-specific RNA binding domain. (embl-heidelberg.de)
Using Affymetrix GENECHIP arrays, specific hybridization can be achieved by direct synthesis of the DNA sequences of interest on arrays. (justia.com)
This approach allows for the locus specific targeting of sequencing in only regions of interest and reduces the overwhelming redundancies required with other random sampling methods. (justia.com)
Methods for the locus specific targeting of sequencing or analysis of regions of interest are disclosed. (justia.com)
Finally, metazoan ORCs do not require binding to specific DNA sequences in order to initiate assembly of a pre-RC. (nih.gov)
Early embryos undergoing rapid cell cleavage (e.g. frogs, flies, sea urchin, and fish) initiate DNA replication with no obvious requirement for any specific DNA sequences, and Drosophila ORC or human ORC can replace Xenopus ORC in frog egg extracts with the same result. (nih.gov)

Yu H, Anderson PJ, Freedman BI, Rich SS, Bowden DW: Genomic structure of the human plasma prekallikrein gene, identification of allelic variants, and analysis in end-stage renal disease. (smpdb.ca)

Also, the algorithm glosses over indels in repeat regions, while Skittle often reveals interesting indel patterns. (biomedcentral.com)
The eight repeats and the N- and C-terminal regions form the Pumilio homology domain (PUM-HD). (embl-heidelberg.de)
The Ampelopsis chloroplast genome is 161,090 bp in length, and includes a pair of inverted repeats of 26,394 bp that are separated by small and large single copy regions of 19,036 bp and 89,266 bp, respectively. (frontiersin.org)
Using the results of pyrosequencing and assembling, we obtained six 5S rDNA- containing contigs with a total length of 140,417 bp, and two sets (pools) of individual 5S rDNA sequences belonging to separate, but closely located genomic regions on the 5BS chromosome. (biomedcentral.com)
The DNA sequencing approach is therefore random and currently does not specifically sample any targeted regions of interest. (justia.com)
Subsequently, development of online databases like GenBank led to the generation of Expressed Sequence Tag (EST) derived-SSRs (eSSRs) which are present in transcribed regions of the genome. (springeropen.com)

Nevertheless, we are sometimes told things like: "The fields of phylogenetics and phylogeography are on the cusp of a revolution, enabled by the rapid expansion of genomic resources and explosion of new genome sequencing technologies. (blogspot.com)

We investigated the utility of multispacer sequence typing and vertebrate hosts, and it causes Q fever in humans and (MST) with 173 C . burnetii isolates. (cdc.gov)

Skittle is especially useful in identifying and analyzing tandem repeats, including repeats not normally detectable by other methods. (biomedcentral.com)
Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. (justia.com)
The methods may be used for genotyping, sequencing and analysis of copy number. (justia.com)
In preferred aspects the methods reduce the requirements for analysis of redundant sequences relative to that of methods that employ random sampling methods. (justia.com)

Conceptually, FISH is a very straightforward technique that essentially consists in hybridizing a DNA probe to its complementary sequence on chromosomal preparations previously fixed on slides. (biosyn.com)

Structural analysis of Drosophila subobscura gypsy elements (gypsyDs). (geneticsmr.com)
The signaling outcome is believed to be a result of structural and sequence-dependent interactions of arrestins with GPCRs and other protein partners. (biomedcentral.com)

Coxiella burnetii is a strict intracellular bacterium with and analysis of endonuclease-digested DNA separated by potential as a bioterrorism agent. (cdc.gov)
We screened 68 spacers in 14 and mucZ gene, which was renamed djlA when the whole isolates and selected the 10 that exhibited the most varia- genome of C . burnetii was sequenced (13,14). (cdc.gov)

2007). Evidence for recent horizontal transfer of gypsy-homologous LTR-retrotransposon gtwin into Drosophila erecta followed by its amplification with multiple aberrations. (geneticsmr.com)
By mapping Expressed Sequence Tags (EST) back to the Drosophila genome, Boutanaev et al . (biomedcentral.com)

Presently the commercial offerings range from systems that claim to achieve from 40 to 100 MB of sequence information in a single run, up to about 1 to almost 2 GB of assembled sequences in a single run. (justia.com)

Analytical, Diagnostic and Therapeutic Techniques and Equipment2

Phenomena and Processes11

Information Science3

Genes28

Recombination6

Nucleotide sequence6

Putative2

Long terminal repeats1

Nucleic acid3

Fully sequenced1

Proteins11

Chromosomes2

Genomes1

Repetitive2

Genomic analysis1

Motifs3

Copies4

Characteristic2

Clusters3

19991

Clonal3

Protein sequences1

Clones1

Duplications1

Loci3

CDNA libraries1

20171

Multilocus sequence1

Genome sequence2

Amino acids2

Species4

Encodes1

Transposable1

Quantitative2

Organization1

Determination3

Mutations1

Yeast1

Genetic4

Strain1

Deletions1

Data12

Evolutionary3

Organisms1

Molecular3

Phylogenetic analysis1

Alignments2

Probes3

Eukaryotes2

Specific6

Allelic1

Regions6

Rapid1

Utility1

Methods4

Complementary1

Structural2

Burnetii2

Drosophila2

Presently1