• tail
  • We compared the kinetics of histone tail substrates for truncated histone lysine demethylases KDM4A and KDM4C containing only the catalytic core (cc) and some combinations were characterized on full length (FL) KDM4A and KDM4C. (jove.com)
  • In addition, PHD2 of JADE1 binds the N-terminal tail of histone H3 within chromatin context irrespective of methylation status. (wikipedia.org)
  • demethylase
  • The histone demethylase KDM3A regulates the transcriptional program of the androgen receptor in prostate cancer cells. (abcam.com)
  • The histone H3K9 demethylase Kdm3b is required for somatic growth and female reproductive function. (abcam.com)
  • Knockout of the Histone Demethylase Kdm3b Decreases Spermatogenesis and Impairs Male Sexual Behaviors. (abcam.com)
  • Beside the NuRD complex, SALL4 is reportedly able to bind to other epigenetic modifiers such as histone lysine-specific demethylase 1 (LSD1), which is frequently associated with the NuRD complex and subsequently gene repression. (wikipedia.org)
  • core histone
  • H3K27me2 is broadly distributed within the core histone H3 and is believed to play a protective role by inhibiting non-cell-type specific enhancers. (wikipedia.org)
  • phosphorylation
  • Histone peptide substrates phosphorylated at T11 could not be demethylated by neither truncated nor full length KDM4A and KDM4C, suggesting that phosphorylation of threonine 11 prevents demethylation of the H3K9me3 mark on the same peptide. (jove.com)
  • D-CMSC were characterized by a reduced proliferation rate, diminished phosphorylation at histone H3 serine 10 (H3S10P), decreased differentiation potential, and premature cellular senescence. (diabetesjournals.org)
  • mitosis
  • HP1 dissociated during mitosis but rebound before the establishment of late replication, and removing HP1 from chromocenters by competition with Me 3 K9H3 peptides did not result in early replication, demonstrating that this interaction is neither necessary nor sufficient for late replication. (rupress.org)
  • Three histone marks - H3K9me2,3, H3K27me1, and H4K20me1 - became abnormally enriched and spread to ectopic positions on the sperm's chromatin before entry into mitosis. (pubmedcentralcanada.ca)
  • Remodeling of chromosomal heterochromatin by EZH2 is also required during cell mitosis. (wikipedia.org)
  • PRC2
  • This transcriptionally repressive state is thought to be due to PRC2/EZH2-EED-mediated H3K27 methylation and subsequent recruitment of PRC1 which facilitates condensation of chromatin and formation of heterochromatin. (wikipedia.org)
  • the PcG repressive complex 1 (PRC1) and the PcG repressive complex 2-4 (PRC2/3/4). (wikipedia.org)
  • Enhancer
  • F1 (10X wash buffer) 20 mlF2 (antibody buffer) 12 mlF3 (detection antibody, 1 mg/ml)* 10 µlF4 (fluoro-developer)* 24 µlF5 (fluoro enhancer)* 24 µlF6 (fluoro-dilution) 8 mlStandard control (100 µg/ml)* 20 µl8 well sample strips (with frame) 98 well standard control strips* 3* For maximum recovery of the products, centrifuge the original vial after thawing prior to opening the cap. (creativebiomart.net)
  • regulation
  • Recent data are accumulating about the roles of diverse histone variants highlighting the functional links between variants and the delicate regulation of organism development. (wikipedia.org)
  • typically
  • Complex multicellular organisms typically have a higher number of histone variants providing a variety of different functions. (wikipedia.org)
  • when a cell divides, the two daughter cells typically contain heterochromatin within the same regions of DNA, resulting in epigenetic inheritance. (wikipedia.org)
  • whereas
  • We conclude that Suv39 activity is required for the fine-tuning of pericentric heterochromatin replication relative to other late-replicating domains, whereas separate factors establish a global replication timing program during early G1 phase. (rupress.org)
  • replication
  • Histones are subdivided into canonical replication-dependent histones that are expressed during the S-phase of cell cycle and replication-independent histone variants, expressed during the whole cell cycle. (wikipedia.org)
  • In this study, we have examined the establishment of late replication for mouse pericentric heterochromatin. (rupress.org)
  • We find that the replication timing program of chromocenters is reestablished coincident with the reorganization of pericentric heterochromatin into chromocenters. (rupress.org)