• cytotoxic T lymphocy
  • Heat shock protein-peptide complexes, reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response and tumor immunity. (nih.gov)
  • The crucial role of ILT3 in the induction of T-cell unresponsiveness was documented in experiments showing that ILT3-overexpressing dendritic cells induce anergy in CD4 + T helper cells and suppress the differentiation of interferon-γ (IFN-γ)-producing CD8 + cytotoxic T-lymphocyte (CTL) ( 22 ). (diabetesjournals.org)
  • vivo
  • Rapamycin is a macrocyclic triene antibiotic produced by Streptomyces hygroscopicus, which was found to have antifungal activity, particularly against Candida albicans, both in vitro and in vivo [C. Vezina et al. (google.de)
  • This finding could be explained by the understanding that antigen-specific circulating CTL display in vivo a quiescent phenotype deprived of most effector functions but gain an effector phenotype that overlaps that of NK cells when activated in vitro through antigen exposure in the presence of proinflammatory stimuli ( 13 ). (aacrjournals.org)
  • Notably, the concentrations of LPS that affect HIV-1 replication in vitro can be easily reached in vivo and may thus affect viral replication in patients with HIV superinfected with bacteria. (rupress.org)
  • and (d) synthetic peptides reconstituted in vitro with gp96 are capable of being taken up and re-presented by macrophage in the same manner as gp96- peptides complexes generated in vivo. (nih.gov)
  • Mice that survived did not show the highest expansion of antigen-specific lymphocytes or the more functionally active effectors, ex vivo or after in vitro culture with the peptide antigen. (aacrjournals.org)
  • In previous studies, we have shown that alloantigen-specific human CD8 + T suppressor (Ts) cells can be generated both in vitro and in vivo by chronic antigenic stimulation. (diabetesjournals.org)
  • antigen-specific
  • The most confounding results of cancer vaccine trials concern the absence of a clear-cut correlation between the clinical responses and the antigen-specific immune response detected in patient-derived T lymphocytes. (aacrjournals.org)
  • CD8 + CD28 − Ts cells from such cultures interacted with priming APCs in an antigen-specific, major histocompatibility complex class I-restricted and contact-dependent manner, inducing the upregulation of the Ig-like transcript (ILT) 3 and ILT4 and inhibiting nuclear factor-κB activation and CD40 signaling in APCs ( 14 - 18 ). (diabetesjournals.org)
  • macrophages
  • Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). (rupress.org)
  • We have studied the effects of LPS on HIV-1 expression in cultures of monocyte-derived macrophages (MDM) 1 and T cells isolated from normal donors, and infected with different strains of HIV-1. (rupress.org)
  • inhibition
  • Our results show that LPS-dependent inhibition of HIV infection affected T lymphocytes, as well as MDM, and involved the release of suppressive factors, most notably, the C-C chemokines RANTES (regulated upon activation, normal T expressed and secreted), macrophage inflammatory protein (MIP)-1α, and MIP-1β. (rupress.org)
  • immunogenic
  • The immunogenicity of HSP preparations has been attributed to peptides associated with the HSPs.The studies reported here demonstrate that immunogenic HSP-peptide complexes can also be reconstituted in vitro.These observations demonstrate that HSPs are CD8+ T cell response-eliciting adjuvants. (nih.gov)
  • The studies reported here demonstrate that immunogenic HSP-peptide complexes can also be reconstituted in vitro. (nih.gov)
  • donor
  • Five patients had progressive disease, two patients had stable disease, and one patient experienced a partial response after receiving donor lymphocyte infusions and IFN-α. (aacrjournals.org)
  • cells
  • Stimulator cell populations that also contain T-cells (Two way mixed lymphocyte reaction) will replicate in the presence of the Responder cells, therefore for a One way mixed lymphocyte reaction, stimulator cells are prevented from replicating by irradiation or treatment with mitomycin C, a DNA crosslinker to prevent cell replication. (wikipedia.org)
  • In 2016, CD19-specific chimeric antigen receptor (CAR)-modified T cells were used to treat patients with relapsed and refractory CD19+ B cell malignancies, including B cell acute lymphoblastic leukemia (B-ALL) harboring rearrangement of the mixed lineage leukemia (MLL) gene with CD19 CAR-T cells. (wikipedia.org)
  • T-cells allostimulated in vitro in the presence of ILT3-Fc inhibited CD40L-induced upregulation of CD40 in human pancreatic islet cells. (diabetesjournals.org)
  • CD4+ Foxp3+ regulatory T cells have been called "naturally occurring" regulatory T cells to distinguish them from "suppressor" T cell populations that are generated in vitro. (wikipedia.org)
  • All T cells begin as CD4-CD8-TCR- cells at the DN (double-negative) stage, where an individual cell will rearrange its T cell receptor genes to form a unique, functional molecule, which they, in turn, test against cells in the thymic cortex for a minimal level of interaction with self-MHC. (wikipedia.org)
  • leukocyte
  • It was at this workshop that Paul Terasaki introduced the microcytotoxicity test for serologic typing, and described the first positive leukocyte antibody crossmatch test associated with hyperacute renal graft rejection. (ihiws.org)
  • The Second Histocompatibility Testing Workshop, organized by J.J. van Rood, was held in Leiden, Netherlands in August of 1965 and brought together 71 participants with an interest in further extending knowledge of human leukocyte antigen systems. (ihiws.org)
  • stimulation
  • Interestingly, the number of tetramer + CD8 + T lymphocytes expanded in vitro after a single cycle of stimulation with the immunodominant TRP-2 peptide was of the highest predictive value. (aacrjournals.org)
  • graft
  • All patients achieved initial mixed hematopoietic chimerism with two patients rejecting their graft and recovering host hematopoiesis. (aacrjournals.org)
  • response
  • This response became quantifiable when incorporating radioactivity, [3H] thymidine or 5-bromo-2'-deoxyuridine (BrdU), into the mixed cell suspension. (wikipedia.org)
  • cell
  • Successful therapy correlated strictly with the skewing of the T-cell receptor repertoire of tetramer-sorted, TRP-2-specific CD8 + T lymphocytes, which showed a preferential α chain usage with a common CDR3 region. (aacrjournals.org)
  • tissue
  • The apparatus of the present invention is particularly, but not exclusively, applicable to the delivery of liquid to and the removal of the contents from wells in a plate, which wells are employed for tissue culture work or, for example, for serological and bacteriological testing procedures. (google.com)
  • The method finds use in tissue cultures, animals and plants. (google.es)
  • Role
  • Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes. (rupress.org)