• cytotoxic T lymphocy
  • Heat shock protein-peptide complexes, reconstituted in vitro, elicit peptide-specific cytotoxic T lymphocyte response and tumor immunity. (nih.gov)
  • The crucial role of ILT3 in the induction of T-cell unresponsiveness was documented in experiments showing that ILT3-overexpressing dendritic cells induce anergy in CD4 + T helper cells and suppress the differentiation of interferon-γ (IFN-γ)-producing CD8 + cytotoxic T-lymphocyte (CTL) ( 22 ). (diabetesjournals.org)
  • assay
  • For research purposes, the MLR cell-based assay continues to provide an in vitro correlate of T cell function. (wikipedia.org)
  • Further characterization of the lymphocytes, accessory cells (dendritic cells, macrophage) and cytokines that participate in the MLR have been done as this assay continues to be used to define mechanisms for understanding cellular immune function in vitro. (wikipedia.org)
  • The workshop was convened for the purpose of comparing the different in vitro assay systems used at the time to define antigens which were described as Hu-1 (Dausset and Ivanyi), LA (Payne and Bodmer), and Four (van Rood). (ihiws.org)
  • Among the assay methods studied in the "wet workshop" format at Duke were serology (including agglutination, cytotoxicity, leukocyte and platelet complement fixation, and mixed hemabsorption), the mixed lymphocyte culture reaction, the normal lymphocyte transfer test, and skin grafting. (ihiws.org)
  • macrophages
  • Human immunodeficiency virus-1 (HIV-1) expression in monocyte-derived macrophages (MDM) infected in vitro is known to be inhibited by lipopolysaccharide (LPS). (rupress.org)
  • We have studied the effects of LPS on HIV-1 expression in cultures of monocyte-derived macrophages (MDM) 1 and T cells isolated from normal donors, and infected with different strains of HIV-1. (rupress.org)
  • peptides
  • The immunogenicity of HSP preparations has been attributed to peptides associated with the HSPs.The studies reported here demonstrate that immunogenic HSP-peptide complexes can also be reconstituted in vitro.These observations demonstrate that HSPs are CD8+ T cell response-eliciting adjuvants. (nih.gov)
  • In addition, mice were immunized (B) with peptides complexed or simply mixed with each of the proteins. (nih.gov)
  • activation
  • Our results show that LPS-dependent inhibition of HIV infection affected T lymphocytes, as well as MDM, and involved the release of suppressive factors, most notably, the C-C chemokines RANTES (regulated upon activation, normal T expressed and secreted), macrophage inflammatory protein (MIP)-1α, and MIP-1β. (rupress.org)
  • cells
  • In 2016, CD19-specific chimeric antigen receptor (CAR)-modified T cells were used to treat patients with relapsed and refractory CD19+ B cell malignancies, including B cell acute lymphoblastic leukemia (B-ALL) harboring rearrangement of the mixed lineage leukemia (MLL) gene with CD19 CAR-T cells. (wikipedia.org)
  • T-cells allostimulated in vitro in the presence of ILT3-Fc inhibited CD40L-induced upregulation of CD40 in human pancreatic islet cells. (diabetesjournals.org)
  • response
  • This response became quantifiable when incorporating radioactivity, [3H] thymidine or 5-bromo-2'-deoxyuridine (BrdU), into the mixed cell suspension. (wikipedia.org)
  • class
  • Klein and his coworkers, finding their locus inseparable from the postulated Ir-1 locus, concluded that the class II antigens they demonstrated on the surfaces of lymphocytes were the product of the Ir-1 locus. (wikipedia.org)
  • cell
  • Successful therapy correlated strictly with the skewing of the T-cell receptor repertoire of tetramer-sorted, TRP-2-specific CD8 + T lymphocytes, which showed a preferential α chain usage with a common CDR3 region. (aacrjournals.org)
  • tissue
  • The apparatus of the present invention is particularly, but not exclusively, applicable to the delivery of liquid to and the removal of the contents from wells in a plate, which wells are employed for tissue culture work or, for example, for serological and bacteriological testing procedures. (google.com)
  • The method finds use in tissue cultures, animals and plants. (google.es)
  • Role
  • Our results raise the possibility that monocytes may play a dual role in HIV infection: while representing a reservoir for the virus, they may contribute to the containment of the infection by releasing factors that suppress HIV replication not only in monocytes but also in T lymphocytes. (rupress.org)