Triose-phosphate isomerase (TPI or TIM) is an enzyme (EC 5.3.1.1) that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. (wikipedia.org)
In humans, deficiencies in TPI are associated with a progressive, severe neurological disorder called triose phosphate isomerase deficiency. (wikipedia.org)
Triose phosphate isomerase deficiency is characterized by chronic hemolytic anemia. (wikipedia.org)
Triose phosphate isomerase is a highly efficient enzyme, performing the reaction billions of times faster than it would occur naturally in solution. (wikipedia.org)
Triose phosphate isomerase is a dimer of identical subunits, each of which is made up of about 250 amino acid residues. (wikipedia.org)
The structure of triose phosphate isomerase contributes to its function. (wikipedia.org)
TIM barrel Triose Phosphate Isomerase deficiency TPI1 Triosephosphate isomerase in interactive 3D at Proteopedia Triosephosphate isomerase (TIM) family in PROSITE Orosz F, Oláh J, Ovádi J (December 2006). (wikipedia.org)
Co-expression of additional EMP enzymes, fructose bisphosphate aldolase (Fba) and triose phosphate isomerase (Tpi), with Pfk I did not enable EMP flux, and resulted in production of glycerol as a side product. (frontiersin.org)
This action stabilizes the enediol intermediate and the other transition states on the reaction pathway. (wikipedia.org)
explored the possibility that the ED pathway limits ATP production and growth in Z. mobilis by attempting to complete an EMP glycolysis pathway. (frontiersin.org)
Reaction3
However, in glycolysis, the use of GAP in the subsequent steps of metabolism drives the reaction toward its production. (wikipedia.org)
In addition to making the reaction kinetically feasible, the TPI loop sequesters the reactive enediol intermediate to prevent decomposition to methylglyoxal and inorganic phosphate. (wikipedia.org)
The reaction sequence in the Calvin-Benson cycle is from triose phosphates to pentose phosphates, the opposite of the typical direction of the non-oxidative PPP. (mdpi.com)
Production1
TPI plays an important role in glycolysis and is essential for efficient energy production. (wikipedia.org)
Phosphate13
Triose-phosphate isomerase (TPI or TIM) is an enzyme (EC 5.3.1.1) that catalyzes the reversible interconversion of the triose phosphate isomers dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate. (wikipedia.org)
In humans, deficiencies in TPI are associated with a progressive, severe neurological disorder called triose phosphate isomerase deficiency. (wikipedia.org)
Triose phosphate isomerase deficiency is characterized by chronic hemolytic anemia. (wikipedia.org)
Triose phosphate isomerase is a highly efficient enzyme, performing the reaction billions of times faster than it would occur naturally in solution. (wikipedia.org)
Other inhibitors include 2-phosphoglycolate, a transition state analog, and D-glycerol-1-phosphate, a substrate analog. (wikipedia.org)
Triose phosphate isomerase is a dimer of identical subunits, each of which is made up of about 250 amino acid residues. (wikipedia.org)
The sequence around the active site residues is conserved in all known triose phosphate isomerases. (wikipedia.org)
The structure of triose phosphate isomerase contributes to its function. (wikipedia.org)
In addition to making the reaction kinetically feasible, the TPI loop sequesters the reactive enediol intermediate to prevent decomposition to methylglyoxal and inorganic phosphate. (wikipedia.org)
The loss of a high-energy phosphate bond and the substrate for the rest of glycolysis makes formation of methylglyoxal inefficient. (wikipedia.org)
TIM barrel Triose Phosphate Isomerase deficiency TPI1 Triosephosphate isomerase in interactive 3D at Proteopedia Triosephosphate isomerase (TIM) family in PROSITE Orosz F, Oláh J, Ovádi J (December 2006). (wikipedia.org)
Pyruvate replaced acetate as the major excreted product, and the following intermediates of glycolysis were excreted in significant amounts: glucose-6-phosphate, fructose-1 ,6-diphos- phate, dihydroxyacetone phosphate, and 3-phosphoglycerate. (nih.gov)