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  • analysis
  • The software has been applied in the analysis of various data sets and its data structures have proven to be highly efficient in capturing and organizing the analytic work flow. (nih.gov)
  • Finally, a number of additional Bioconductor packages successfully build on the infrastructure provided by flowCore, open new avenues for flow data analysis. (nih.gov)
  • The heightened sensitivity and multicolor capabilities of the flow cytometer detection system also enable additional applications, including antibody screening, cytotoxicity studies and biomarker analysis. (technologynetworks.com)
  • Unlike high-content analysis, high-content screening implies a level of throughput which is why the term "screening" differentiates HCS from HCA, which may be high in content but low in throughput. (wikipedia.org)
  • A high-capacity analysis machine can measure dozens of plates in the space of a few minutes like this, generating thousands of experimental datapoints very quickly. (wikipedia.org)
  • A flow cytometer allows simultaneous multiparametric analysis of the physical and chemical characteristics of up to thousands of particles per second. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. (wikipedia.org)
  • The ability to quantify these has led to flow cytometry being used in a wide range of applications, including but not limited to: Monitoring of CD4 count in HIV Diagnosis of various cancers Analysis of aquatic microbiomes Sperm sorting Measuring telomere length Until the early 2000s, flow cytometry could only measure a few fluorescent markers at a time. (wikipedia.org)
  • Rapid/high throughput: In multiplex analysis, a 100-plex assay can be analyzed in every 30 seconds. (wikipedia.org)
  • technology
  • High-throughput biology serves as one facet of what has also been called "omics research" - the interface between large scale biology (genome, proteome, transcriptome), technology and researchers. (wikipedia.org)
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
  • cell populations
  • Flow cytometry is a very powerful tool that has been used for decades, and allows for multiparametric readouts at the single cell level within heterogeneous cell populations,' Joslin states. (slas.org)
  • Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (wikipedia.org)
  • method
  • This method is advantageous over cell-based ELISA, in that the signal to noise ratios tend to be much higher, and cell preparations do not have to be homogenous because the population of interest can be gated by a population-specific marker. (antibody.com)
  • An overview is presented of recent developments of X‐ray computed tomography as an affordable, simple method of high spatial resolution to study 3D anatomy both in vivo and ex vivo. (spectroscopynow.com)
  • High content screening includes any method used to analyze whole cells or components of cells with simultaneous readout of several parameters. (wikipedia.org)
  • At a cellular level, parallel acquisition of data on different cell properties, for example activity of signal transduction cascades and cytoskeleton integrity is the main advantage of this method in comparison to the faster but less detailed high throughput screening. (wikipedia.org)
  • At the same time, microfluidic qPCR methods are providing a flow cytometry-like method of quantifying 48 or more RNA molecules per cell. (wikipedia.org)
  • However, the method has a notoriously high false-positive rate, which makes it necessary to verify the identified interactions by co-immunoprecipitation. (wikipedia.org)
  • The Tandem affinity purification (TAP) method allows the high-throughput identification of proteins interactions. (wikipedia.org)
  • biological
  • High-content screening (HCS) in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells. (wikipedia.org)
  • Finally, diagnosis using flow cytometry data can be aided by supervised learning techniques, and discovery of new cell types of biological importance by high-throughput statistical methods, as part of pipelines incorporating all of the aforementioned methods. (wikipedia.org)
  • An event is either an actual biological cell or some other mass that was large enough to trigger the data acquisition capturing device of the flow cytometer instrument. (wikipedia.org)
  • capability
  • A common variation involves linking the analytical capability of the flow cytometer to a sorting device, to physically separate and thereby purify particles of interest based on their optical properties. (wikipedia.org)
  • diagnosis
  • Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. (wikipedia.org)
  • allows
  • This allows for a throughput of approximately 40,000 wells per day with less than one full time employee overseeing the system. (slas.org)
  • High array density/multiplex: Compared to flat microarrays, SAT allows one to perform parallel measurements. (wikipedia.org)
  • Allows the processing of high-throughput mass spectrometry data using tools such as the X! (wikipedia.org)
  • microfluidic
  • The first label-free high-frequency impedance flow cytometer based on a patented microfluidic "lab-on-chip", Ampha Z30, was introduced by Amphasys (2012). (wikipedia.org)
  • researchers
  • The mandate of C-CAMP is to provide high-end platform technologies and expertise to all the academic and industrial scientific researchers in and around India. (wikipedia.org)
  • system
  • The recent reported high-throughput flow cytometry can sample a 96-well plate in 1 minute, and theoretically, the 100-plex assay with this system can be analyzed in less than 1 second, or potentially deliver 12 million samples per day. (wikipedia.org)
  • software
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • Since then, FCS became the standard file format supported by all flow cytometry software and hardware vendors. (wikipedia.org)
  • molecules
  • Generally, interactions between GSH and other molecules with higher relative electrophilicity deplete GSH levels within the cell. (wikipedia.org)
  • cells
  • Progressive high-content screening systems have been demonstrated to process up to 50,000 wells/day on a routine basis with screening campaigns into the millions of wells, but this has been limited to adherent cells. (slas.org)
  • Flow cytometry is a widely used technique that enables the measurement of different optical properties of individual cells within large populations of cells in a fast and automated manner. (embopress.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • Such a reaction is probable due to the relatively high concentration of glutathione in cells (up to 7 mM in the liver). (wikipedia.org)
  • In high concentrations, the organic molecule diethyl maleate fully depleted GSH levels in cells. (wikipedia.org)
  • development
  • A brief review on the development of super-atmospheric pressure ion sources, including high-pressure electrospray, field desorption and superheated ESI, and the strategies to. (spectroscopynow.com)
  • Identification
  • Target identification has been defined as the rate limiting step in chemical genetics/high-content screening. (wikipedia.org)
  • For population identification, tools are available to aid traditional manual identification of populations in two-dimensional scatter plots (gating), to use dimensionality reduction to aid gating, and to find populations automatically in higher-dimensional space in a variety of ways. (wikipedia.org)
  • quality control
  • We are working to increase access and control of the cytometer in an automated manner so that we can increase the throughput and quality control of the screens we run. (slas.org)
  • Methods
  • Highlights in this large body of work include the development of fluorescence lifetime imaging methods, the application of high sensitivity vibrational spectroscopies to small volume analysis and the development integrated optics for point-of-care diagnostic technologies. (wikipedia.org)
  • data
  • This equipment provides high quality reproducible data with minimal effort. (selectscience.net)
  • We could predict with high accuracy (greater than or equal to 80 %) a wide range of immunological statuses such as healthy, transplantation recipient, and lymphoid cancer, suggesting as a proof of principle that diversity profiling can recover a large amount of immunodiagnostic fingerprints from immune repertoire data. (beds.ac.uk)
  • At a cellular level, parallel acquisition of data on different cell properties, for example activity of signal transduction cascades and cytoskeleton integrity is the main advantage of this method in comparison to the faster but less detailed high throughput screening. (wikipedia.org)
  • High-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with IT-systems for the analysis and storage of the data. (wikipedia.org)
  • So, for the CASY technology, as the cell flow cytometry, it can present data of each cell as a cell size distribution graph, which has 2 variables, the change in cell volume and that in cell viability. (wikipedia.org)
  • analysis
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • A high-capacity analysis machine can measure dozens of plates in the space of a few minutes like this, generating thousands of experimental datapoints very quickly. (wikipedia.org)
  • High-content screening (HCS), also known as high-content analysis (HCA) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. (wikipedia.org)
  • less
  • Due to being single-use only, larger than their MEMS counterparts, and the requirement of clean room facilities, high material and processing costs make silicon-based bio-MEMS less economically attractive. (wikipedia.org)
  • cell-based
  • PPD Laboratories' central lab performs cell-based assays and flow cytometry under good clinical laboratory practice (GCLP) and/or clinical laboratory improvement amendment (CLIA) regulations. (ppdi.com)
  • Applications
  • Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. (wikipedia.org)
  • Early flow cytometers were, in general, experimental devices, but technological advances have enabled widespread applications for use in a variety of both clinical and research purposes. (wikipedia.org)
  • However, there are some drawbacks to using silicon-based devices in biomedical applications such as their high cost and bioincompatibility. (wikipedia.org)
  • Instruments
  • Soon after, flow cytometry instruments were developed, including the Cytofluorograph (1971) from Bio/Physics Systems Inc. (later: Ortho Diagnostics), the PAS 8000 (1973) from Partec, the first FACS (Fluorescence-activated cell sorting) instrument from Becton Dickinson (1974), the ICP 22 (1975) from Partec/Phywe and the Epics from Coulter (1977/78). (wikipedia.org)
  • thousands
  • Automatic colony pickers pick thousands of microbial colonies for high throughput genetic screening. (wikipedia.org)
  • In high-density oligonucleotide SNP arrays, hundreds of thousands of probes are arrayed on a small chip, allowing for many SNPs to be interrogated simultaneously. (wikipedia.org)
  • digital
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • levels
  • The undifferentiated stem cell is characterized by high levels of both alkaline phosphatase and aldehyde dehydrogenase. (selectscience.net)
  • Generally, interactions between GSH and other molecules with higher relative electrophilicity deplete GSH levels within the cell. (wikipedia.org)
  • rate
  • This ensures a very high recovery rate since analytes do not adhere to any carrier or matrix structure. (wikipedia.org)
  • drug
  • Charles River has a range of orthogonal approaches that can deliver high-quality, validated hit compounds for your drug discovery project. (scienceexchange.com)