• protein
  • In biotechnology, flow cytometry is a laser- or impedance-based, biophysical technology employed in cell counting, cell sorting, biomarker detection and protein engineering, by suspending cells in a stream of fluid and passing them through an electronic detection apparatus. (wikipedia.org)
  • The screening of protein-protein interactions refers to the identification of protein interactions with high-throughput screening methods such as computer- and/or robot-assisted plate reading, flow cytometry analyzing. (wikipedia.org)
  • Phenotype
  • High-content screening (HCS), also known as high-content analysis (HCA) or cellomics, is a method that is used in biological research and drug discovery to identify substances such as small molecules, peptides, or RNAi that alter the phenotype of a cell in a desired manner. (wikipedia.org)
  • analysis
  • The software has been applied in the analysis of various data sets and its data structures have proven to be highly efficient in capturing and organizing the analytic work flow. (nih.gov)
  • Finally, a number of additional Bioconductor packages successfully build on the infrastructure provided by flowCore, open new avenues for flow data analysis. (nih.gov)
  • The new NovoCyte Flow Cytometer addresses a full range of multi-parameter cellular analysis research needs with high performance at a low investment cost, making it more attainable for researchers to have benchtop flow cytometers in their labs. (bucher.ch)
  • CytoSPADE is a high-performance implementation of an interface for the Spanning-tree Progression Analysis of Density-normalized Events algorithm for tree-based analysis and visualization of this high-dimensional cytometry data. (pubmedcentralcanada.ca)
  • Unlike high-content analysis, high-content screening implies a level of throughput which is why the term "screening" differentiates HCS from HCA, which may be high in content but low in throughput. (wikipedia.org)
  • A high-capacity analysis machine can measure dozens of plates in the space of a few minutes like this, generating thousands of experimental datapoints very quickly. (wikipedia.org)
  • A flow cytometer has five main components: a flow cell, a measuring system, a detector, an amplification system, and a computer for analysis of the signals. (wikipedia.org)
  • The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. (wikipedia.org)
  • proteins
  • These engineered antibodies have been constructed in the hunt for novel therapeutic drugs equipped with enhanced immunoprotective abilities, such as engaging immune effector functions, effective development of fusion proteins, efficient tumor and tissue penetration, and high-affinity antibodies directed against conserved targets. (frontiersin.org)
  • The Tandem affinity purification (TAP) method allows the high-throughput identification of proteins interactions. (wikipedia.org)
  • analyze
  • However, practical approaches to analyze and visualize cytometry data at this scale are only now becoming available. (pubmedcentralcanada.ca)
  • Modern flow cytometers are able to analyze many thousand particles per second, in "real time," and, if configured as cell sorters, can actively separate and isolate particles at similar rates having specified optical properties. (wikipedia.org)
  • advances
  • Early flow cytometers were, in general, experimental devices, but technological advances have enabled widespread applications for use in a variety of both clinical and research purposes. (wikipedia.org)
  • Cytometer
  • A common variation involves linking the analytical capability of the flow cytometer to a sorting device, to physically separate and thereby purify particles of interest based on their optical properties. (wikipedia.org)
  • Acquisition is mediated by a computer physically connected to the flow cytometer, and the software which handles the digital interface with the cytometer. (wikipedia.org)
  • An event is either an actual biological cell or some other mass that was large enough to trigger the data acquisition capturing device of the flow cytometer instrument. (wikipedia.org)
  • methods
  • This platform will foster the development of novel analytic methods for flow cytometry. (nih.gov)
  • Moreover, the described methods are adapted to work in 96-well format, allowing for medium and high-throughput studies. (jove.com)
  • Dr. Ailles' most recent scientific contributions include the identification of tumor-initiating cell populations in head and neck and ovarian cancer, and development of novel methods for generating patient-derived cultures from ccRCC patients with high efficiency. (utoronto.ca)
  • High throughput cell biology has a definite focus on the cell, and methods accessing the cell such as imaging, gene expression microarrays, or genome wide screening. (wikipedia.org)
  • High throughput cell biology dictates methods that can take routine cell biology from low scale research to the speed and scale necessary to investigate complex systems, achieve high sample size, or efficiently screen through a collection. (wikipedia.org)
  • Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (wikipedia.org)
  • technology
  • IntelliCyt™ Corporation develops and markets innovative high throughput cell screening solutions using flow cytometry technology for drug discovery and life science research. (bucher.ch)
  • High-throughput biology serves as one facet of what has also been called "omics research" - the interface between large scale biology (genome, proteome, transcriptome), technology and researchers. (wikipedia.org)
  • High-content" or visual biology technology has two purposes, first to acquire spatially or temporally resolved information on an event and second to automatically quantify it. (wikipedia.org)
  • More recently, the new technology of mass cytometry replaces fluorophores with rare-earth elements detected by time of flight mass spectrometry, achieving the ability to measure the expression of 34 or more markers. (wikipedia.org)
  • robust
  • In this note, we present CytoSPADE, a robust, modular, cross-platform and high-performance implementation of the SPADE algorithm and an accompanying graphical user interface that improves performance by 12-19-fold relative to the SPADE prototype, enabling gigabyte-scale datasets to be analyzed and effectively visualized in hours or minutes, not days. (pubmedcentralcanada.ca)
  • diagnosis
  • Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in basic research, clinical practice and clinical trials. (wikipedia.org)
  • cell
  • Our current work focuses on these questions in head-and-neck squamous cell carcinoma, high grade serous ovarian cancer, and clear cell renal cell carcinoma. (uhnresearch.ca)
  • High Throughput Flow Cytometry for Cell Surface Profiling. (uhnresearch.ca)
  • Poly(beta-amino ester)s (PBAEs) have shown great potential as gene delivery reagents because they are easily synthesized and they transfect a wide variety of cell types with high efficacy in vitro. (jove.com)
  • Classical High throughput screening robotics are now being tied closer to cell biology, principally using technologies such as High-content screening. (wikipedia.org)
  • High-content screening (HCS) in cell-based systems uses living cells as tools in biological research to elucidate the workings of normal and diseased cells. (wikipedia.org)
  • At a cellular level, parallel acquisition of data on different cell properties, for example activity of signal transduction cascades and cytoskeleton integrity is the main advantage of this method in comparison to the faster but less detailed high throughput screening. (wikipedia.org)
  • Mack Fulwyler was the inventor of the forerunner to today's flow cytometers - particularly the cell sorter. (wikipedia.org)
  • The flow cell has a liquid stream (sheath fluid), which carries and aligns the cells so that they pass single file through the light beam for sensing. (wikipedia.org)
  • Generally, interactions between GSH and other molecules with higher relative electrophilicity deplete GSH levels within the cell. (wikipedia.org)
  • method
  • High-throughput screening (HTS) is a method for scientific experimentation especially used in drug discovery and relevant to the fields of biology and chemistry. (wikipedia.org)
  • However, the method has a notoriously high false-positive rate, which makes it necessary to verify the identified interactions by co-immunoprecipitation. (wikipedia.org)
  • researchers
  • The mandate of C-CAMP is to provide high-end platform technologies and expertise to all the academic and industrial scientific researchers in and around India. (wikipedia.org)
  • software
  • Using robotics, data processing/control software, liquid handling devices, and sensitive detectors, high-throughput screening allows a researcher to quickly conduct millions of chemical, genetic, or pharmacological tests. (wikipedia.org)
  • Since then, FCS became the standard file format supported by all flow cytometry software and hardware vendors. (wikipedia.org)
  • technologies
  • C-CAMP acts as the provider and developer of high-end technologies in life sciences to enable scientific activity and entrepreneurship. (wikipedia.org)
  • cells
  • Flow cytometers operate by hydrodynamically focusing suspended cells so that they separate from each other within a fluid stream. (wikipedia.org)
  • In high concentrations, the organic molecule diethyl maleate fully depleted GSH levels in cells. (wikipedia.org)