• Drug screening efforts have traditionally relied primarily on time-consuming or computationally demanding techniques like ELISA-type assays and high-content imaging cytometry. (labmanager.com)
  • At CHI's recent "High-Content Flow Cytometry" meeting, Dr. Robinson talked about the ability to automate flow cytometry-based high-content screens and data processing to perform multiplexed high-content analysis in real-time, running multiple assays and samples in parallel, and to miniaturize the technology to conserve resources and reduce costs. (genengnews.com)
  • Currently, data generated from microarray, flow cytometry and metabolomics-based (i.e. mass spectrometry) assays are supported. (bioconductor.org)
  • This approach is easily incorporated into most existing phagocytosis assays and allows for reproducible results with high sensitivity. (lu.se)
  • The assignment comprises transcriptomic (bulk and sc-mRNA-seq), epigenomic (ATACseq, CUT&RUN), proteomic (Olink assays), and compositional, metabolic and immunophenotypic profiling (high dimensional cytometry). (lu.se)
  • Flow cytometry bioinformatics is the application of bioinformatics to flow cytometry data, which involves storing, retrieving, organizing and analyzing flow cytometry data using extensive computational resources and tools. (wikipedia.org)
  • Here we present CytoPy, a Python framework for automated analysis of high dimensional cytometry data that integrates a document-based database for a data-centric and iterative analytical environment. (biorxiv.org)
  • Flow cytometry bioinformatics requires extensive use of and contributes to the development of techniques from computational statistics and machine learning. (wikipedia.org)
  • Open standards, data and software are also key parts of flow cytometry bioinformatics. (wikipedia.org)
  • In response to these shortcomings, a cross-disciplinary effort has given birth to a new approach often termed „cytometry bioinformatics‟, to leverage complex computer algorithms and machine learning to automate analysis and improve the investigator‟s ability to extract meaning from high dimensional data. (biorxiv.org)
  • Identification of inhibitors of vacuolar proton-translocating ATPase pumps in yeast by high-throughput screening flow cytometry. (unm.edu)
  • Flow cytometry-based high-throughput screening of synthetic peptides for palladium adsorption. (bvsalud.org)
  • In summary, our study proposes an alternative high-throughput method for analyzing the Pd(II) adsorption capacity of individual yeast cells , enabling the screening of specific peptides / proteins with high Pd(II) affinity from extensive libraries . (bvsalud.org)
  • Yokogawa's high-content analysis systems (HCA), also known as high-content screening systems (HCS), address a range of research applications from basic science to drug discovery screening. (yokogawa.com)
  • CellVoyager CV8000 is the most advanced high-content screening system. (yokogawa.com)
  • With its expandability, 4 cameras, 5 lasers and an optional built-in pipettor, the system permits increasingly complex assay development and high-content screening. (yokogawa.com)
  • Our high content analysis (HCA) systems are the best solutions for a range of research applications from basic science to drug discovery screening. (yokogawa.com)
  • High-content analysis is a principle methodology for so-called phenotypic screening in drug discovery and development process. (yokogawa.com)
  • Assessing a vast number of compounds is time consuming, therefore, acquiring a large number of high resolution cellular images with fast scanning speed is critically important for drug screening. (yokogawa.com)
  • Our high-content confocal imaging systems based on the CSU are the ideal solution for drug screening using elaborated 3D and live cell assay systems as well as 2D fixed samples. (yokogawa.com)
  • High detection sensitivity, throughput, and 384-microwell plate compatibility of the Octet ® RH16 system have also made it a favorite for small molecule library screening and analysis of large cross-competition binding campaigns. (sartorius.com)
  • Therapeutics development, antibody screening, and biomarker discovery demand maximal productivity to reduce time-to-results, which can greatly benefit from high-throughput, high parametric flow analysis interfaced with robotic automation. (bio-rad.com)
  • Critical for helping scientists accelerate scientific and drug discoveries in both academia and industry, the ZE5 Cell Analyzer is a high performance, automation-ready flow cytometer with the ideal combination of high-throughput screening and high parameter cell analysis capabilities. (bio-rad.com)
  • These advances enable high-throughput phenotypic screening and structure-activity relationship screening (SAR) in areas like drug discovery, therapeutics development, and biomarker discovery. (labmanager.com)
  • Flow cytometry has only recently become a viable method for high-throughput screening. (labmanager.com)
  • The increasing use of automation and miniaturization is lowering the cost of flow cytometry-based high-content analysis in primary and secondary drug screening, making it possible to run larger screening campaigns. (genengnews.com)
  • Due to our unique combination of engineered mouse models, proprietary immunization techniques, and premium high-throughput and high-content screening capabilities, we specialize in high homology targets, constrained epitope targeting, and tough cell surface targets, all of which exist within the immuno-oncological antibody research space. (news-medical.net)
  • Highthroughput in vitro screening strategies including high content imaging, electric cell impedance sensing, and flow cytometry were employed to evaluate a set of pre- and post-incinerated ONCs for acute effects and fate of the monolayer post-exposure. (cdc.gov)
  • An integrated high-throughput in vitro screening strategy, using high content imaging and traditional in vitro methods, represents a rapid pulmonary epithelial toxicity assessment approach to prioritize ENMs for further evaluation and serves to inform 'prevention-by-design' material development strategies. (cdc.gov)
  • Here we review the key recent innovations that ensure reliable, high-throughput, automated workflows. (labmanager.com)
  • Accelerate high-throughput flow and mass cytometry workflows with fully automated sample preparation. (curiox.com)
  • Cell Surface Profiling Using High-Throughput Flow Cytometry: A Platform for Biomarker Discovery and Analysis of Cellular Heterogeneity. (stembook.org)
  • Importantly, this opportunity will expose you to a broad range of experimental techniques, such as flow cytometry, cell culture, high-dimensional data analysis and advance your technical skills to the next level! (joinhandshake.com)
  • Execute cell-staining experiments and perform sample analysis using flow cytometry. (joinhandshake.com)
  • The rapid growth in the multidimensionality and throughput of flow cytometry data, particularly in the 2000s, has led to the creation of a variety of computational analysis methods, data standards, and public databases for the sharing of results. (wikipedia.org)
  • The ability to quantify these has led to flow cytometry being used in a wide range of applications, including but not limited to: Monitoring of CD4 count in HIV Diagnosis of various cancers Analysis of aquatic microbiomes Sperm sorting Measuring telomere length Until the early 2000s, flow cytometry could only measure a few fluorescent markers at a time. (wikipedia.org)
  • The rapid increase in the dimensionality of flow cytometry data, coupled with the development of high-throughput robotic platforms capable of assaying hundreds to thousands of samples automatically have created a need for improved computational analysis methods. (wikipedia.org)
  • The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
  • The Department of Ophthalmology owns two pieces of equipment that are useful for high throughput analysis. (utsouthwestern.edu)
  • Paired with our advanced analysis software, our HCA systems provide high quality 3D imaging for enhanced live cell analysis. (yokogawa.com)
  • High content analysis (HCA) identifies the phenotype or target reaction of small molecules, peptides, RNAi, drug cocktail or, antibodies. (yokogawa.com)
  • Based on high-resolution microscopic images, high content analysis (HCA) enables you to detect cellular phenotypic changes at a molecular level, so the technology can be utilized as a powerful tool to study effects of physiologically active substances, such as compounds, siRNA, peptides and antibodies, on cultured cells, tissue samples and whole organisms, like zebrafish. (yokogawa.com)
  • Yokogawa high-content analysis imagers have balanced these generally conflicting requirements at high level of perfomance. (yokogawa.com)
  • User friendly high content analysis software CellPathfinder, with its versitile functionalities, powerfully supports the analysis of various phnotype changes and target reaction. (yokogawa.com)
  • All the technologies are packed into the Yokogawa high-content analysis system! (yokogawa.com)
  • From IgG titer to epitope binning, the Octet ® RH16 system offers label-free biomolecular interaction analysis with state-of-the-art high throughput platforms that facilitate the rapid characterization and development of biologics drug molecules. (sartorius.com)
  • The Octet ® platform's rapid high throughput protein analysis combined with the anti-CHO HCP and Residual Protein A kits provide biologics developers with a fast and easy-to-use assay for contaminant testing. (sartorius.com)
  • Learn the principles of exosome detection using flow cytometry, and access a range of useful resources on small particle analysis, including a handy protocol. (bio-rad.com)
  • Technological advances over the last two decades have turned flow cytometry into a powerful high-throughput tool for a broad range of large-scale applications requiring rapid multi-parameter analysis. (labmanager.com)
  • While it has always been ideal for multiparametric analysis of cells based on their physical characteristics and surface protein expression, flow cytometry has traditionally been too slow in processing large sample numbers to gain widespread use in high-volume applications. (labmanager.com)
  • The demand for high-throughput flow cytometry solutions offering rapid multiparameter analysis is not surprising, given the context. (labmanager.com)
  • However, developing truly high-throughput capabilities requires the systematic removal of process bottlenecks from sample loading through data analysis. (labmanager.com)
  • Automation provides a solution to managing the huge datasets produced with multiparametric high-content analysis. (genengnews.com)
  • Using this approach, a biological sample can be analyzed in parts-exploiting the ability to separate individual cells for analysis using flow cytometry-with the data then combined to piece together the complete picture. (genengnews.com)
  • In addition, Dr. Robinson presented an example of multicolor flow analysis to demonstrate the ability to perform cytomics studies for pathway analysis. (genengnews.com)
  • We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
  • Cytometry analysis has grown in recent years with the expansion in the maximum number of parameters that can be acquired in a single experiment. (biorxiv.org)
  • Despite the success of numerous algorithms and published packages to replicate and outperform traditional manual analysis, widespread adoption of these techniques has yet to be realised in the field of cytometry. (biorxiv.org)
  • Cytometry data analysis has undergone a paradigm shift in response to the growing number of parameters that can be observed in any one experiment. (biorxiv.org)
  • To this end, a computational approach to analysis of cytometry data can take one of two strategies: to separate single cell data into groups or classifications, which then form the variables (often descriptive statistics of the obtained groups) the investigator uses to test their hypothesis, or directly model the acquired distribution of single cell data with respect to a chosen endpoint. (biorxiv.org)
  • Professor Summers is internationally recognised for his research excellence, which focuses on two areas: metrologies for cell analysis (cytometry) and the development of nanoparticle-based diagnostics and therapeutics (nanomedicine). (swansea.ac.uk)
  • Finally, the facility is equipped with a laminar flow hood to conduct chemistry to create biomaterials and micropatterned surfaces for stem cell culture, as well as subsequent analysis by imaging and flow. (ca.gov)
  • While high throughput imaging and automated analysis techniques are extremely useful, they suffer by removing the trained researcher from actually examining the majority of the images. (biomedcentral.com)
  • High-resolution structural analysis showed that an R28 domain with IgI3-like fold. (lu.se)
  • High-resolution structural analysis showed that an R28 domain with IgI3-like fold binds to the N-terminal domain of CEACAM1. (lu.se)
  • We thank staff at the Flow Cytometry Facility and High-Throughput Single Cell Analysis (HTSCA) Facility for technical assistance. (lu.se)
  • Consequently, SYNMIKRO invested in several devices with different key aspects regarding the analysis of fluorescently-tagged molecules for its Flow Cytometry. (uni-marburg.de)
  • In this webinar, Miltenyi Biotec and Toxys will highlight the potential of using flow cytometry for high content toxicity analysis. (labroots.com)
  • Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. (bdbiosciences.com)
  • T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. (bdbiosciences.com)
  • Our data analysis work involves mathematical modeling , and high-performance computing and we develop computational methods for large scale genetic data. (lu.se)
  • Flow cytometry is used for single-cell analysis of cell populations and monitoring of population phenotypic heterogeneity as well as for preparative sorting of individual cells and subpopulations. (lu.se)
  • Focus of the course will be on getting practical experience of flow cytometry for single-cell analysis and cell sorting of microorganisms. (lu.se)
  • By using dose-response curve principles for both experimental design and analysis, it is possible to increase the sensitivity and robustness, leading to accurate quantification of phagocytosis that is comparable across experiments and systems.Here, we describe how to quantify phagocytosis using flow cytometry with a robust, high-throughput, and easy-to-use approach. (lu.se)
  • The laboratory holds resources for a diversity of immunochemical analysis tools, for determination of affinity and reaction rate constants of molecular interactions (using surface plasmon resonance (Biacore) technology), and for high throughput multicolour flow cytometry. (lu.se)
  • The high throughput format enables a time-saving, unbiased acquisition and analysis of cells in a multiwall format, or on slides. (lu.se)
  • Analyze flow cytometry data from multi-color flow cytometry experiments. (joinhandshake.com)
  • Overall the use of flow cytometry to analyze individual cell populations and evaluate the effects of drugs on cell function allows you "to ask the what-if questions" and to begin to integrate pathways, he said. (genengnews.com)
  • In the course we will cover the most important applications of microbial flow cytometry and provide a clear view of what kind of information can be obtained, as well as how to analyze and interpret the data. (lu.se)
  • Assay speed, throughput, exceptional reproducibility, and dedicated software tools make Octet ® platforms ideal for analyzing large antibody libraries. (sartorius.com)
  • Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
  • Individual experimental designs for flow cytometry must be optimized, including antibody dilution and incubation time. (novusbio.com)
  • Our traditional hybridoma discovery approach triages antibody candidates in a high-throughput manner by specificity, affinity, and oftentimes functionality early in the workflow. (news-medical.net)
  • Leads Discovery & Optimization (LDO) is a diverse group of scientists and engineers, providing critical assay information to therapeutic research centers (TRCs) throughout research and early development (R&ED). We are seeking a highly motivated and innovative intern to join the High-Throughput Flow Cytometry team within LDO. (joinhandshake.com)
  • These days, immunohistochemical staining is just one of several established immunostaining techniques, including enzyme-linked immunosorbent assay, flow cytometry, immuno-electron microscopy, and Western blotting. (news-medical.net)
  • Flow cytometry offers benefits to the technicians who choose to use it, including high sample throughput speed, making it an attractive option as an immunostaining assay. (news-medical.net)
  • A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. (bdbiosciences.com)
  • Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. (bdbiosciences.com)
  • A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. (bdbiosciences.com)
  • The Investigation / Study / Assay (ISA) tab-delimited format is a general purpose framework with which to collect and communicate complex metadata (i.e. sample characteristics, technologies used, type of measurements made) from experiments employing a combination of technologies, spanning from traditional approaches to high-throughput techniques. (bioconductor.org)
  • Combined with portable systems, the magnetic barcode assay holds promise to become a sensitive, high-throughput, and low-cost platform for point-of-care diagnostics. (cdc.gov)
  • Truly high-throughput capabilities are now possible off-the-shelf through advanced complimentary instrument and software solutions. (labmanager.com)
  • In addition, our analytical and sorting flow cytometry capabilities complement the imaging equipment by providing high throughput cell fluorescence measurements. (ca.gov)
  • Quantification of gene delivery efficiency and gene targeting has been greatly aided with the flow cytometry and high throughput imaging capabilities of the CIRM facility. (ca.gov)
  • Developed as a commercial technology by DVS Sciences, which Fluidigm purchased in 2014, mass cytometry combines the capabilities of flow cytometry and atomic mass spectrometry, allowing it to measure large numbers of proteins in single cells with high throughput. (genomeweb.com)
  • Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. (bdbiosciences.com)
  • Flow cytometry is a high-throughput technique for counting and studying microscopic particles in suspension by passing them through a laser and an electronic detection apparatus. (lu.se)
  • bacteria culture is the gold standard in TB detection for its highest sensitivity, but requires weeks to obtain results. (cdc.gov)
  • MTB), and thereby enabling high detection specificity and versatility. (cdc.gov)
  • Describing the benefits of using flow cytometry to study disease specificity as it relates to cell types and signaling pathways, Dr. Kritzik reported that it is possible to measure at least 10 parameters in 5,000-10,000 single cells per second. (genengnews.com)
  • Flow cytometry can be used to quantify the number and proportion of specific cell types in a heterogeneous mixture, by examining the expression of proteins often expressed on the surface of cells. (bio-rad.com)
  • Fluorescence intensity of the pH-sensitive carboxyfluorescein derivative 2,7-bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) was monitored by high-throughput flow cytometry in living yeast cells. (unm.edu)
  • Mass cytometry overcomes limitations of spectral overlap and auto-fluorescence inherent to fluorescence-based measurements. (nature.com)
  • In particular, we have a two photon and visible confocal fluorescence microscope and a high throughput fluorescence imager that are being increasingly used. (ca.gov)
  • Flow cytometry is a laser-based technology that allows the simultaneous measurement of fluorescence in single cells at different wavelengths reporting the cellular concentration of several fluorescently-tagged molecules, and of other physical or biological parameters such as cell size or granularity in a statistically robust fashion. (uni-marburg.de)
  • Herein, a semi-quantitative method to measure Pd(II) adsorption capacity of single cells has been presented by analyzing side scatter (SSC) intensity in flow cytometry . (bvsalud.org)
  • For instance, you've got a protein that would normally be expressed on the surface of the cell-if you take it off the surface of the cell and put it onto a plate, then potentially you're changing the conformation of that protein," explains Richard Cuthbert, PhD, flow cytometry product manager at Bio-Rad. (labmanager.com)
  • Using Fluidigm's Helios mass cytometry instrument, the researchers measured several dozen protein markers in 3.5 million immune cells, identifying 17 different tumor-associated macrophage (TAM) phenotypes and 22 T cell phenotypes. (genomeweb.com)
  • Flow cytometry have important applications in many fields including fundamental and applied microbiology, bioprocess monitoring, protein engineering, plant biology and marine biology. (lu.se)
  • Computational methods exist to assist in the preprocessing of flow cytometry data, identifying cell populations within it, matching those cell populations across samples, and performing diagnosis and discovery using the results of previous steps. (wikipedia.org)
  • Methodologically, we combine large-scale genomics and high-throughput phenotyping with advanced computational and experimental approaches. (lu.se)
  • Schramm T, Lempp M, Beuter D, Sierra SG, Glatter T, Link H (2020), High-throughput enrichment of temperature-sensitive argininosuccinate synthetase for two-stage citrulline production in E. coli. (uni-marburg.de)
  • The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
  • Advances in flow cytometry and sequencing have transformed immunology by enabling large numbers of parameters to be quantified on single cells in high-throughput. (washington.edu)
  • Investigating FlowSight® imaging flow cytometry as a platform to assess chemically induced micronuclei using human lymphoblastoid cells in vitro. (swansea.ac.uk)
  • Flow cytometry and related methods allow the quantification of multiple independent biomarkers on large numbers of single cells. (wikipedia.org)
  • Finally, diagnosis using flow cytometry data can be aided by supervised learning techniques, and discovery of new cell types of biological importance by high-throughput statistical methods, as part of pipelines incorporating all of the aforementioned methods. (wikipedia.org)
  • At the same time, microfluidic qPCR methods are providing a flow cytometry-like method of quantifying 48 or more RNA molecules per cell. (wikipedia.org)
  • Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder ® HiFi DNA Assembly and NEBridge ® Golden Gate Assembly . (neb.com)
  • Complement-dependent cytotoxic crossmatch (CDCXM) and flow cytometric crossmatch (FXCM) are both common methods to predict donor-recipient compatibility - the potential success of an organ transplant. (curiox.com)
  • The lab led by Assistant Prof. Camila Consiglio employs high-throughput multiomics technologies and advanced computational methods to explore sex differences in human immunity. (lu.se)
  • The candidate will apply systems immunology methods to comprehensively profile the human immune system using various high-throughput technologies. (lu.se)
  • A flow cytometric immunoassay for β2-microglobulin in whole blood. (bdbiosciences.com)
  • Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. (bdbiosciences.com)
  • The feasibility of automated spatial layout to allow comparison and discrimination of high throughput sub-cellular imaging has been demonstrated. (biomedcentral.com)
  • High-throughput automated fluorescent microscope imaging technologies enable the experimental determination of a protein's sub-cellular localization and its dynamic trafficking within a range of cellular contexts. (biomedcentral.com)
  • In response to this there has been an increased effort to develop computational methodologies for handling high-dimensional single cell data acquired by flow or mass cytometry. (biorxiv.org)
  • The Laminar Wash AUTO 1000 System eliminates time-consuming and labor-intensive manual pipetting and handling by automating the complete surface and intracellular staining workflow for flow and mass cytometry. (curiox.com)
  • The Laminar Wash AUTO 1000 is a completely hands-off automated platform to dispense, fix/permeabilize, stain, wash, and transfer samples for flow and mass cytometry. (curiox.com)
  • Published this week in Cell , the study used mass cytometry to characterize T cells and macrophages in samples from 73 ccRCC patients and five healthy matched kidney samples, identifying immune phenotypes associated with progression free-survival as well as potential biomarkers that could be useful for purposes including immunotherapy development. (genomeweb.com)
  • Flow cytometers operate by hydrodynamically focusing suspended cells so that they separate from each other within a fluid stream. (wikipedia.org)
  • Flow Cytometry is an extremely effective method of recognizing and assessing characteristics of cells or particles. (bio-rad.com)
  • Flow cytometry may be used to separate and sort cells based on various detectable characteristics, which has many uses in research. (bio-rad.com)
  • Instrument blocking has formed another major concern, according to Richard, who reports further innovation in sample pumps along with increased pressure and speed in flow cells within the ZE 5 Cell Analyzer to mitigate this problem. (labmanager.com)
  • Peter Krutzik, Ph.D., a senior scientist in Gary Nolan's group at the Baxter Lab in genetics, pharmacology, microbiology, and immunology at Stanford University, described the use of phosphor flow cytometry to profile perturbations in signaling networks when peripheral blood cells are exposed to drug compounds. (genengnews.com)
  • Dr. Kritzik and colleagues are using phosphor-flow cytometry to study three phosphoproteins-all linked to disease and all potential drug targets-in subsets of peripheral blood cells. (genengnews.com)
  • Aliquot up to 1 x 10 6 cells per 100 μL in Flow Cytometry Staining Buffer if performing cell surface staining. (novusbio.com)
  • Resuspend cell pellets in 150 μL of Flow Cytometry Permeabilization/Wash Buffer I. Add 1 μg blocking IgG per 1 x 10 6 cells and let stand for 15 minutes at room temperature. (novusbio.com)
  • Wash cells ONCE with 2 mL of Flow Cytometry Permeabilization/Wash Buffer I. Centrifuge suspension at 1,300 RPM (500 x g maximum) and 4°C for 5 minutes. (novusbio.com)
  • When the physical and chemical characteristics of cells or particles are sought to be determined, flow cytometry is often the most suitable method. (news-medical.net)
  • Like flow cytometry, high-content imaging cytometry is multiparametric, but it requires considerable processing power for analyzing and storing large image data files, and capturing rare events is difficult. (labmanager.com)
  • Data standards include the widely adopted Flow Cytometry Standard (FCS) defining how data from cytometers should be stored, but also several new standards under development by the International Society for Advancement of Cytometry (ISAC) to aid in storing more detailed information about experimental design and analytical steps. (wikipedia.org)
  • Where cytometry is used for data acquisition, the typical objective is to discern differences between groups of subjects or experimental conditions, or to identify a phenotype that correlates with an experimental or clinical endpoint. (biorxiv.org)
  • Bio-Rad's high-throughput ZE 5 Cell Analyzer solved this with an integrated wash station that travels with the probe, cleaning it thoroughly between each sample. (labmanager.com)
  • The Celigo is a bench-top, high throughput, micro-well plate based, bright field and fluorescent imaging system. (utsouthwestern.edu)
  • The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
  • Our facility is designed to support human embryonic stem cell (hESC) culture, including high quality cell culture space equipped with biosafety cabinets, incubators, cryogenic storage, and a standard microscope. (ca.gov)
  • Flow cytometry data is in the form of a large matrix of intensities over M wavelengths by N events. (wikipedia.org)
  • Dr. Robinson also discussed how data-extraction issues have historically been a stumbling block in the use of flow cytometry in drug discovery. (genengnews.com)
  • For unconjugated antibodies, be sure to check the data sheet for any appropriate concentrations validated for use in flow cytometry. (novusbio.com)
  • These data show that, compared to historic controls, patients treated with FCR followed by rituximab maintenance have high-quality responses with fewer relapses and improved OS. (haematologica.org)
  • We make use of high-resolution, high-throughput flow cytometry, advanced imaging techniques, gene expression data as well as crosslinking data from population based registries. (lu.se)
  • After incubation, data is acquired through flow cytometry. (lu.se)
  • For population identification, tools are available to aid traditional manual identification of populations in two-dimensional scatter plots (gating), to use dimensionality reduction to aid gating, and to find populations automatically in higher-dimensional space in a variety of ways. (wikipedia.org)
  • The following flow cytometry staining protocol for intracellular molecules using detergents to permeabilize cell membranes has been developed and optimized by Bio-Techne. (novusbio.com)
  • Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. (bdbiosciences.com)
  • Using this method , six strains with high Pd adsorption capacities have sorted from a yeast library with metal -binding peptides displayed (up to 107 strains ) based on SSC signal intensity. (bvsalud.org)
  • In addition to the classical DSRT, our high throughput flow cytometry-based DSRT allows to evaluate drug responses at the population level. (helsinki.fi)
  • It shows that the technology to establish a high-throughput vaccine formulations should target. (cdc.gov)
  • He is co-inventor of the HyperCyt high throughput flow cytometry platform and co-founder of IntelliCyt. (selectbiosciences.com)
  • We will present our high-content imaging devices with which you can elevate your organoid research to the next level. (yokogawa.com)
  • The initial resistance that the high-content/high-throughput community had to face has been overcome, and now most in the field acknowledge that flow cytometry is enormously high throughput and very high content. (genengnews.com)
  • Traditional flow cytometers need to fill the sample line up to the interrogation point with material from a single sample, which is time consuming. (labmanager.com)
  • It can be scaled by expansion of the split-pool process and effectively renders sequencing instruments as versatile multi-parameter flow cytometers. (nature.com)
  • Moreover, inclusion of a high throughput imaging cytometer allows for many of these same comparisons to be made without the need for trypsinization and extensive cell processing. (utsouthwestern.edu)
  • Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
  • To successfully carry out a large proportion of the applications mentioned above, you will need a high performance flow cytometer such as the ZE5 Cell Analyzer. (bio-rad.com)
  • The core technology, the microlens-ehananced dual Nipokow disk in Yokogawa Confocall Scanner or Confocal Spinning disc Unit (CSU), makes it possible to capture high quality images with remarkably short aquisition time. (yokogawa.com)
  • Flow cytometry is a key technology in the field of cancer research, but presents challenges for flow cytometry instrumentation, especially when studying solid tumors or dealing with a high volume of samples. (bio-rad.com)