High throughput cells cultured. Medical search. Frequent questions

Collectively, both arms of this Module add scientific value to the work of vision scientists by reducing the time and effort required for basic cell culture assays such as viability and cell cycle assessments. (utsouthwestern.edu)
and a Nexcelom Cellometer for rapid determination of cell number, cell viability, transfection efficiency, and cell cycle assays. (utsouthwestern.edu)
It consists of 4 fluorescent channels (UV to far red) and has extensive applications including bright field cell counting, wound-healing and cell growth assays, cell line development, induced pluripotent stem cell (iPSC) reprogramming, 3D invasion and migration modeling, immune-oncology, virology, and fluorescent-based assays. (utsouthwestern.edu)
Spheroids and organoids are replacing standard cell culture in many traditional cell-based assays. (corning.com)
However, to maximize the cost savings and time efficiency, scientists must be aware of the factors that influence whether or not cell imaging returns high quality data, for both multiwell plate assays and for creating a digital model from z-stack layers. (corning.com)
Develop, perform and analyze immuno-cell based assays data to determine the productivity of the platform process as well as to expedite project timeline. (biospace.com)
Recommended incubator shaker configuration for high-throughput screening assays. (eppendorf.com)
Screening assays involve the exposure of PBMCs to new potential antibodies, e.g. to test for toxicity with cell-based assays or ELISA. (eppendorf.com)
Screening using cell-based assays has been performed for many years, but recent technologies allow screening of cells that are alive and changing. (genengnews.com)
Cell-based assays are becoming increasingly popular for small molecule discovery in high-throughput screening. (genengnews.com)
As cell-based assays are used more commonly in robotic high-throughput compound screening, cells themselves have become critical reagents. (genengnews.com)
We experimented with cells division-arrested with low level mitomycin C treatment and demonstrated that they perform with better consistency than non-division arrested counterparts in high-content screening imaging assays, says Zhong Zhong, vp of development at CMT. (genengnews.com)
This expanding panel of cell-based receptor assays, which currently includes 54 targets, is being offered by CMT for custom compound profiling to evaluate compound selectivity over a wide range of likely GPCR targets. (genengnews.com)
We developed Redistribution cell-based assays for use in both primary screening and lead optimization of compounds targeting GPCRs. (genengnews.com)
Redistribution assays are independent of the imaging platform and have been shown to give consistent results on a number of instruments on the market, including: IN Cell 3000 (GE Healthcare), Opera (Evotec Technologies), ArrayScan (Cellomics), Pathway HT (BD Biosciences), Discovery1 (Molecular Devices), and Icyte (CompuCyte). (genengnews.com)
Redistribution assays can be multiplexed by either expressing two targets in the same cell line labeled with different colors, or by mixing different assay cell lines directly in the microtiter plate well. (genengnews.com)
Cellular transformation/tumorigenicity assays using cultures of cells in semi-solid media (soft agar or Matrigel) has been well established for cancer research 1,2,5 . (moleculardevices.com)
3D assays were formerly labor-intensive and inconsistent due to the subjective definitions of colonies and, non-suitable for high-throughput screening. (moleculardevices.com)
and high-throughput assays, such as next-generation sequencing, transcriptomics, mass spectrometry and proteomic analysis. (mayo.edu)
Automation of the cell culture, assays, and analysis can provide the tools necessary to facilitate and scale up the use of organo-on chip systems. (moleculardevices.com)
THUNDER Imager for 3D Cell Culture & 3D Live Cell are designed for imaging of cell culture assays. (news-medical.net)
Cell assays and primary cells provide the tools researchers need to study the oxidative process and its association with skin aging. (promocell.com)
Compared to other currently used assays, HINT offers improved throughput and allows for the possibility of directly analyzing respiratory samples from infected patients, eliminating the need to grow the virus in cell culture. (cdc.gov)
The assignment comprises transcriptomic (bulk and sc-mRNA-seq), epigenomic (ATACseq, CUT&RUN), proteomic (Olink assays), and compositional, metabolic and immunophenotypic profiling (high dimensional cytometry). (lu.se)

Topics covered include various high-throughput genomics technologies (genome sequencing, DNA microarrays, proteomics), quantitative modeling of transcriptional and post-transcriptional regulatory networks, synthetic biology, and the world of RNA structure, dynamics, and function. (columbia.edu)
The candidate will apply systems immunology methods to comprehensively profile the human immune system using various high-throughput technologies. (lu.se)

The incorporation of a flow cytometer and a cell sorter also reduces the cost associated with recurrent use of the main flow cytometry facility on campus. (utsouthwestern.edu)
The goals for advances in flow cytometry are clear: measure as many relevant target molecules per cell as quickly as possible. (nature.com)
Fluorescence and isotope tagging are the principal means for measuring antibody binding to cells in flow cytometry. (nature.com)
Connors and his team first used flow cytometry to isolate B cells from an HIV-infected donor. (the-scientist.com)

It merges microfluidics, a set of technologies used for the manipulation of small fluid volumes (μL, nL, pL) within artificially fabricated microsystems, and cell culture, which involves the maintenance and growth of cells in a controlled laboratory environment. (wikipedia.org)
Microfluidics has been used for cell biology studies as the dimensions of the microfluidic channels are well suited for the physical scale of cells (in the order of magnitude of 10 micrometers). (wikipedia.org)
Therefore, it is important to design the microfluidics system for cell culture in a manner that minimizes cell stress situations. (wikipedia.org)
Given the small dimensions in microfluidics, laminar flow can be achieved, allowing manipulations with the culture system to be done easily without affecting other culture chambers. (wikipedia.org)
Microfluidics entrepreneur Mark Gilligan provides a perspective on the development of single-cell sequencing technologies. (rsc.org)
Nucleic acid cytometry using droplet microfluidics identifies and sorts single cells, virus, or free molecules based on specific "keyword" sequences. (rsc.org)
Spheroid culture can be achieved in diverse environments including low-attachment microplates, bioreactors, and microfluidics culture systems. (sigmaaldrich.com)
Synthetic biologists have used microfluidics for DNA assembly, cell-free expression, and cell culture, but a combination of expense, device complexity, and reliance on custom set-ups hampers their widespread adoption. (nature.com)
Microfluidics enables single-cell manipulation and analysis, reaction volumes of nano- or picoliters (thereby reducing costs), high-throughput execution of parallel experiments, automated routine liquid handling, integration of multiple biological processes in a single system, and programmability for complex protocols. (nature.com)
Microfluidics gives promising strategies for aligning cells in physiologically related configurations to recapitulate human organ performance. (ncbcs.org)

Cell-based anticancer drug screening generally utilizes rapidly proliferating tumour cells grown as monolayer cultures. (researchgate.net)
The aim of this study was to examine the potential usefulness of 3-D cultured tumour cells for anticancer drug screening. (researchgate.net)
Screening of monolayer and spheroid cultures was found to identify different sets of hit compounds. (researchgate.net)
Therefore, we suggest the high-throughput screening (HTS) system based on ES cells in this study. (molcells.org)
In conclusion, the novel HTS system using ES cells can by useful for developing mod-els for drug discovery as well as toxicological screening in the near future. (molcells.org)
Droplet-based single cell platform allows functional screening and sorting of desirable TCR T cells to accelerate development of adoptive T cell therapies. (rsc.org)
Spheroid culture is often favored for high-throughput compound screening in drug development and toxicology, where spheroids present more biologically-relevant models than 2D cultures. (sigmaaldrich.com)
Going beyond the cell monolayer is driving deeper and faster research into personalized medicine and rapid drug screening. (corning.com)
Advances in cell culture consumables mean that spheroid and organoid culture is now highly amenable to high content screening . (corning.com)
We dedicated the Cell Culture Screening Solution exactly for these workflow steps - as it supports reproducible assay conditions regardless of the vessel location inside the incubator. (eppendorf.com)
High-throughput screening has been around for a while, but it has only been in the last few years that doing so on a cellular level has been feasible. (genengnews.com)
Advanced high-throughput screening technologies require standardized, consistent cell culture as reagents to generate reliable results. (genengnews.com)
www.cmt-inc.net), makes it possible to use frozen assay-ready cells for screening as if they were ordinary assay reagents like enzymes or membrane preparations. (genengnews.com)
For cell-based screening, it is possible to uncouple the cell production process from the screening process. (genengnews.com)
This problem could be significantly mitigated by coordinately screening multiple tissue types simultaneously in a high-throughput fashion during upstream tier 1 screening. (inknowvation.com)
However, with existing bioluminescent reporter technology, this is simply not possible because the current market of bioluminescent reporter cells being applied toward toxicology screening relies upon a firefly luciferase gene construct that must be provided with a chemical substrate to activate its light emission response, resulting in only marginally informative single time point snapshots of potential toxicological interactions. (inknowvation.com)
This process is frustratingly exacerbated by an unfortunate dichotomy whereby the inexpensive in vitro cell culture systems used for tier 1 screening contribute to failures due to their inabiliy to model the complexity and parallel systems interaction inherent in whole animal models (which are responsible for up to 92% of new compound failures at the clinical level) and because they are not capable of demonstrating species-specific effects. (inknowvation.com)
To overcome these detractions and develop an improved tier 1 screening system that reduces the cost and time required for new compound evaluation, 490 BioTech proposes to develop a panel of multiple continuously bioluminescent human cell lines that will permit the simultaneous monitoring of each line to ascertain both the individual effects of compound treatment as well as the downstream effects of a compound's biotransformed metabolic breakdown products in real-time. (inknowvation.com)
Topics covered will include high-throughput assay development, screening, chemical library creation, high-throughput chemistry, affinity purification of target proteins and target validation, protein microarrays, molecular evolution, protein engineering and synthetic biology. (columbia.edu)
In this study, we describe a method compatible with highthroughput screening, in which compound effects on cell growth in semi-solid media were measured and quantitated using high-content imaging and 3D analysis. (moleculardevices.com)
By screening single hybrid cells for those that produce the antibody of interest, researchers can develop a cell line generating virtually unlimited amounts of highly specific antibodies. (the-scientist.com)
The automated method is extensively applicable for compound screening and using Organ-on -chip technology in a high-throughput manner. (moleculardevices.com)
This project's objective was to conduct rapid high-throughput screening of cultured human kidney cells to identify potential adverse outcome pathways associated with renal injury. (cdc.gov)
High-throughput screening for cytotoxicity, mitochondrial polarization, and intracellular reactive oxygen species (ROS) levels of exposed cells was performed by multiplex fluorescence and image analysis using a high content imaging platform. (cdc.gov)
MultiPark has made a substantial investment in a High Content Screening machine. (lu.se)
The state-of-the-art apparatus offers a high throughput system for screening at sub-cellular level. (lu.se)

RNeasy Plus Kits are next generation QIAGEN RNA extraction kits, combining high-quality RNA purification with effective gDNA removal with unique gDNA Eliminator columns or plates. (qiagen.com)
The RNeasy Plus 96 Kit is for high-throughput purification of total RNA from cultured cells grown in 96-well plates. (qiagen.com)

NaCl Celecoxib 136.9 KCl 5.4 CaCl2 1.5 MgCl2 1.0 NaHCO3 23.8 ethylenediaminetetraacetic acidity 0.01) while reported previously [15 16 2 Cell isolation and tradition migration of rat cavernous soft muscle tissue cells was measured through the use of Matrigel with the next adjustments [13]. (sciencepop.org)
However, significant challenges such as accessibility of macaque brain tissue and the lack of a robust neural cell isolation and culture protocol have hampered its progress. (springernature.com)
In our recent work published on Nature Protocols, we describe a simple and reproducible method for the isolation and culture of functional adult and aged macaque brain cells. (springernature.com)
Isolation of primary cells from the hippocampus provides an opportunity to study neurogenesis, particularly capturing the neurogenesis process. (springernature.com)
In addition, our primary cell isolation protocol was adapted for high-yield neurons. (springernature.com)
Isolation of high-quality living neurons from the adult and aged is one of the major bottlenecks for the broad application. (springernature.com)
Quantum barcoding (QBC) avoids isolation of single cells by building cell-specific oligo barcodes dynamically within each cell. (nature.com)
Cell culture isolation of SARS-CoV-2 is possible, but the Centers for Disease Control and Prevention (CDC) recommends that clinical laboratories not attempt this unless it is performed in a biosafety level 3 (BSL-3)-certified laboratory. (medscape.com)

PDE) family members mRNA in RPSMCs had been weighed against patterns in rat aortic soft muscle tissue cells (RASMCs) by usage of quantitative real-time change transcription polymerase string reaction. (sciencepop.org)
To determine whether the gene amplification in cells is morphologically and/or genetically related to the primary tumor requires quantitative evaluation of a large number of cell nuclei from continuous meaningful structures such as milk-ducts, tumors, etc., located relatively far from the primary tumor. (nih.gov)
This four-credit course will present a quantitative description of the molecular networks that underlie the myriad phenotypes of living cells, from yeast to human. (columbia.edu)

The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
Single-cell RNA-sequencing (scRNA-seq) has revolutionized biomedical research by enabling the in-depth analysis of cell-to-cell heterogeneity of tissues with unprecedented resolution. (rsc.org)
When cells are not grown on supports, they may form 3D aggregates called spheroids, which secrete their own ECM to become more like native solid tissues. (sigmaaldrich.com)
Stem cells reside within most tissues throughout the lifetimes of mammalian organisms. (biomedcentral.com)
Total RNA of high purity and yield is obtained from a wide range of cultured cells and easy-to-lyse tissues. (qiagen.com)
Our facilities provide the opportunity to study protein structure, molecular probes and drug design, system biology and molecular interactions in cells and tissues. (lu.se)
Although current treatment modalities offer high levels of success for many conditions, an ideal form of therapy might consist of regenerative approaches in which diseased or necrotic pulp tissues are removed and replaced with healthy pulp tissue to revitalize teeth. (bvsalud.org)
All tissues originate from stem cells. (bvsalud.org)
A stem cell is commonly defined as a cell that has the ability to continuously divide and produce progeny cells that differentiate (develop) into various other types of cells or tissues. (bvsalud.org)
Postnatal stem cells have been sourced from umbilical cord blood, umbilical cord, bone marrow, peripheral blood, body fat, and almost all body tissues, including the pulp tissue of teeth 8 . (bvsalud.org)

The expression vectors are transfected into cells, and the resulting antibodies are purified and tested for activity. (the-scientist.com)
While previous researchers have obtained HIV-neutralizing antibodies using fluorescent probes to pull out antigen-specific memory B cells from a sample of blood, Connors and his team wanted to discover new HIV-neutralizing antibodies, without being limited only to those that are specific for known antigens. (the-scientist.com)
But rather than cloning the antibody heavy- and light-chain genes from each cell, as in a typical expression cloning set-up, Connors's group distributed the isolated cells in 384-well plates, where they cultured them in the presence of feeder cells and signaling molecules that help to keep the B cells alive and also stimulate them to secrete antibodies. (the-scientist.com)

The Arabidopsis thaliana homolog of yeast BRE1 has a function in cell cycle regulation during early leaf and root growth. (helsinki.fi)

Embryonic stem (ES) cells can be valuable for monitoring differentiation processes and for improving applications in basic developmental biology. (molcells.org)
Perfusion in microfluidic cell culture is important to enable long culture periods on-chip and cell differentiation. (wikipedia.org)
Highly characterized cryopreserved human colonic organoids and a step-by-step organoid culture protocol for epithelial intestinal organoid differentiation from iPS cells. (sigmaaldrich.com)
Lipids, proteins, carbohydrates and DNA are all susceptible to oxidative damage and this damage can change gene transcription along with cell proliferation and differentiation and is involved in several diseases including diabetes and obesity, cancer and neurological disorders (Kregel, 2007). (promocell.com)

An ultrahigh-throughput single-cell miRNA assay is developed by a continuous-flow microfluidic process employing isothermal amplification to amplify the target miRNA signal. (rsc.org)

Compared with adherent cells grown in 2D monolayers, 3D growth conditions are believed to more accurately reflect the natural environment of cancer cells and span the gap between 2D cultures and animal studies 3,5 . (moleculardevices.com)
In contrast to soluble chemicals, cadmium oxide particles on a cm2/ml media basis, but are only ~50 particles can settle, diffuse, and aggregate differentially accord- times more potent when differences in delivery to adherent cells are ing to their size, density, and surface physicochemistry. (cdc.gov)
Solid know how of Microsoft office tools is required as well as an experience with cell culture (adherent cells). (lu.se)

This provides an uninterrupted stream of visual data over the lifetime of the reporter cell as it interacts and reacts to compound exposure at both its pre- and post-biotransformed states. (inknowvation.com)
The drug discovery process requires that upwards of 10,000 molecules be screened for each new lead compound developed and relies upon an expensive and time consuming combination of in vitro cell culture-based and in vivo whole animal-based models to identify, validate, and ensure the safety of any resulting potential therapeutic agents. (inknowvation.com)

Spheroids of EMT-6 mammary tumor cells were markedly more resistant to different exposure doses of Adriamycin (ADR) than monolayer cells in exponential or plateau growth phases. (researchgate.net)
Statistical analysis of genetic changes within cell nuclei that are far from the primary tumor would help determine whether such changes have occurred prior to tumor invasion. (nih.gov)
The study of two cases of ductal carcinoma in situ in breast tissue supports the biological observation regarding the existence of a preferential intraductal invasion, and therefore a common origin, between the primary tumor and the gene amplification in the cell-nuclei lining the ductal structures in the breast. (nih.gov)
The growing interest in developing methods for personalized medicine, in which tumor cells from individual patients are tested for sensitivity to a panel of drugs 4 , has led to a need for more relevant, time-sensitive studies. (moleculardevices.com)

The Cellomics machine allows you to not only image live cells, but to keep those cells alive for both short- and long-term experiments in a controlled environment similar to that of a tissue culture incubator. (lu.se)

By seeding the stem cell-derived kidney cells on this matrix-coated chip, the cells can be grown into controlled shapes and arrangements. (corning.com)

The gene expression analysis confirmed that 3D trophoblast cell cultures and especially HTR8/SVneo spheroids showed considerable similarities with the gene expression profile of primary placental tissue. (ufz.de)
Free radicals, both the reactive oxygen species (ROS) and reactive nitrogen species (RNS), can lead to damage of biomolecules which then affects gene expression, cell metabolism and distinct physiological functions. (promocell.com)
High-throughput RNA sequencing (RNA-seq) has revolutionized our understanding of gene expression. (cdc.gov)

The cells fragments had been then positioned and embedded in 48-well plates covered with Matrigel supplemented with platelet-derived development factor-BB and incubated for 5 times (Fig. 1). (sciencepop.org)
These reduced cell numbers make studying non-dividing or slow dividing cells (e.g., stem cells) easier than traditional culture methods (e.g., flasks, petri dishes, or well plates) due to the smaller sample volumes. (wikipedia.org)
With minimal instrumentation (four 96-well plates and a multichannel pipette), cell-specific codes are added to each tagged molecule within cells through sequential rounds of classical split-pool synthesis. (nature.com)
For example, U-bottom spheroid culture plates not only optimize growth conditions but also create uniform cell growth that sits centrally in each well. (corning.com)
Omitting cell scraping does not compromise the performance of non-targeted and targeted GC-MS analysis, but enables metabolome analysis of cell culture on smaller well sizes down to 96-well plates. (frontiersin.org)
Ease of stacking, multiple sealing options as well as high chemical and temperature resistance make these plates excellent, multipurpose tools. (capitolscientific.com)
The plates fulfill current guidelines: SBS1, EN ISO 9001/13485. (capitolscientific.com)
Easy and safe sealing: Eppendorf® Deepwell Plates 96 and 384 are high-quality consumables to ease high-throughput operation and handling. (capitolscientific.com)
To prepare spheroid cultures in semi-solid media, human colon carcinoma HCT116 cells were pre-mixed with a solution of 4.25 mg/mL cold Matrigel (Corning) diluted in growth media and plated into 96-well half-area plates (Greiner) at a density of \~500 cells/well for a total volume of 25 μL. (moleculardevices.com)

pp. 558-572) demonstrate that recombinant human hyaluronan and proteoglycan link protein 1 (rhHAPLN1) significantly reduces the extended airspaces of the emphysematous alveoli by increasing the levels of TGF-β receptor I and SIRT1/6, as a previously unrecognized mechanism in human alveolar epithelial cells, and consequently mitigates COPD. (molcells.org)
Once researchers have cloned the pairs of heavy- and light-chain genes naturally found in the cells, they can produce recombinant versions of each antibody in the lab. (the-scientist.com)

Receptor tyrosine kinases including EGFR, HER-2 and c-Met and non receptor tyrosine kinase including Jaks are selectively expressed in primary tissue and metastatic tumour of advanced ovarian cancer cells. (otago.ac.nz)
Despite wealth information of these tyrosine kinase expressions and activation, little is known about the biological activity of tyrosine kinases in ovarian cancer cells are present in ascitic fluid. (otago.ac.nz)
This project is investigating the expression and activation of tyrosine kinases in ovarian cancer cells isolated from ascitic fluid. (otago.ac.nz)
In addition, the ascitic fluid contains various of cell types including white blood cells, mesenchymal cells, red blood cells and ovarian cancer cells (single cells and clusters). (otago.ac.nz)
In recent years, a few studies have started to investigate the biological activity of ascitic fluid,which is believed to influence behavior of ovarian cancer cells. (otago.ac.nz)
Even though the exact insight mechanisms of how ascitic fluid exert to ovarian cancer cells is poorly defined, my research suggests that ascitic fluid can increase expression of oncogenic proteins in ovarian cancer cells and reduce the cellular uptake of targeted drugs. (otago.ac.nz)

this can make studying certain cell-cell interactions more accessible. (wikipedia.org)
While the molecular biology of the two viruses has been characterized in great detail, the absence of robust cell culture models for HBV and/or HDV infection has limited the investigation of virus-host interactions. (mdpi.com)
In contrast, our substrate-free, autobioluminescent reporter cell lines emit light continuously and can modulate the output level of this signal in real time in response to cytotoxic interactions. (inknowvation.com)

In a recently published Nature Communications paper, a iBB team led by Margarida Diogo, in collaboration with Perpetua Pinto do Ó at i3S, engineered a unique organ-like model from stem cells that recreates several stages of embryonic development of the human heart. (scoop.it)
We explore the method on three circuits for haematopoiesis and embryonic stem cell development for commitment and reprogramming scenarios and illustrate how the method can be used to determine sequential steps for onsets of external factors, essential for efficient reprogramming. (lu.se)
Based on their origin, there are two main types of stem cells - embryonic stem cells (ES cells) and postnatal or adult stem cells (AS cells). (bvsalud.org)
Embryonic stem cells are stem cells derived from the inner cell mass of an early, preimplantation stage embryo known as a blastocyst. (bvsalud.org)

Identification of biomarkers to stratify cell lines, characterization of important processes as epithelial-mesenchymal transition (EMT), and the use of three-dimensional (3D) cultures as alternative models could be useful for cell line classification. (oncotarget.com)
The HI test is the traditional method used by flu scientists to conduct antigenic characterization. (cdc.gov)

Hit compounds from such screens are not necessarily effective on hypoxic and slowly proliferating cells in 3-D tumour tissue. (researchgate.net)
Tumour-associated macrophages (TAMs) are one of the most abundant immune cells present in the TME, and play a key role both in the development and in the progression of HCC. (bvsalud.org)

A cocktail of small-molecule inhibitors is added during perfusion and dissociation to protect neural cells from cytotoxicity by blocking receptors or channels. (springernature.com)

The microbiome, the "other ge- species have no representative in nome" or "second genome" of the culture collections. (who.int)

For example, cytotoxicity studies are faster and provide more comprehensive data with higher throughput. (corning.com)
In summary, AFFF showed similar kidney cell cytotoxicity to C8 PFAS -exposed cells suggesting PFAS within AFFF contributes to kidney cell cytotoxicity. (cdc.gov)

Epithelial cells tend to show higher proliferative rates, and mesenchymal cells show an increase in EMT related markers, especially when cultured in 3D conditions. (oncotarget.com)
These dental stem cells are considered mesenchymal stem cells (MSCs) and possess different levels of capacities to become specific tissue forming cells. (bvsalud.org)

As of first of January 2022, StemTherapy and MultiPark have decided to merge the former iPSC, CRISPR and vector platforms into the new Cell and Gene Therapy Core . (lu.se)

We present a method enabling simultaneous, ultra-high throughput single-cell barcoding of millions of cells for targeted analysis of proteins and RNAs. (nature.com)
Small organic molecules function in energy production and creating building blocks for the components of cells and can also be used to perturb the functions of proteins directly. (columbia.edu)
Below is a non-exhaustive list of in-house infrastructures that are categorized into three overarching themes: bio-imaging, proteins, genes & cells and other resources. (lu.se)

To address this issue, we have designed an integrated image analysis software system for high-throughput segmentation of nuclei. (nih.gov)

By using a selective disaggregation technique after intact spheroids had been exposed to the drug it was possible to show directly that the inner spheroid cells were most resistant (D0 = 0.25 μ/106 cells). (researchgate.net)
this will not only show penetration but also indicate cell viability and spheroid structure. (corning.com)
The resulting spheroid cultures were treated with anti-cancer compounds for 5 days. (moleculardevices.com)
Multi-parametric outputs were generated using a customized analysis module (Figure 3) and the readouts for each individual spheroid included measurements for nuclear count, size, volume, intensity, live cells per spheroid, whole cell volume and diameter, spheroid diameter, and average intensities of cellular markers (calcein AM, Phalloidin, or Hoechst). (moleculardevices.com)
HeLa cell spheroid stained with Alexa Fluor 568 Phalloidin (Actin) and YOYO 1 iodide (Nucleus). (news-medical.net)
The spheroid growth rates of JEG3, BeWo and HTR8/SVneo cell lines were similar among each other and were significantly larger in comparison to primary trophoblast spheroids. (ufz.de)

New metabolomics applications of ultra-high resolution and accuracy mass spectrometry can provide thousands of detectable isotopologues, with the number of potentially detectable isotopologues increasing exponentially with the number of stable isotopes used in newer isotope tracing methods like stable isotope-resolved metabolomics (SIRM) experiments. (mdpi.com)

Transcriptomic profiling of T-cell populations in non-muscle invasive and muscle invasive bladder cancer. (lu.se)

3D cell culture models may be generally classified into two principal categories based on method: 1) scaffold-based methods using hydrogels or structural scaffolds and 2) scaffold-free approaches using freely floating cell aggregates, typically referred to as spheroids. (sigmaaldrich.com)
Literature review: This review summarizes current knowledge, barriers, and challenges in the clinical use of adult stem cells, scaffolds, and growth factors for the development and evaluation of regenerative endodontic therapies. (bvsalud.org)

Our facilities provide the opportunity to study molecules, cells, organs and entire organisms. (lu.se)

Because cells in vivo interact with their environment in three dimensions, 3D cell culture tools, reagents, and techniques have led to the creation of more predictive in vitro cell models for diverse applications and disciplines including cancer research, drug discovery, neuroscience, and regenerative medicine. (sigmaaldrich.com)
Thus, it has become essential to produce cell reagents with high consistency and quality. (genengnews.com)

It's a fusion between stem cell and bioengineering fields," explains Freedman. (corning.com)
An article collection in Stem Cell Research & Therapy . (biomedcentral.com)
This series covers biophysical regulation of stem cell fate and function in the context of tissue development. (biomedcentral.com)
Advances in the fields of stem cell biology, biomaterials, and tissue engineering over the last decades have brought the possibility of constructing tissue substitutes with a broad range of applications in reg. (biomedcentral.com)
In this regard, tissue engineers have sought to leverage native mechanotransduction pathways to enhance in vitro stem cell. (biomedcentral.com)
Currently unique to the market, the new technology deployed in THUNDER Imagers makes visualization and analysis of large volume, thick specimens ideal for many biomedical applications where they are required, including regenerative medicine, cancer, and stem cell research to decode 3D biology in real time. (news-medical.net)
those of cell reprogramming, thereby avoiding exhaustive trial- energy landscape, deterministic models, and-error simulations with rate equations for different stem cell commitment, reprogramming parameter sets. (lu.se)
This explains why researchers are now focusing attention on developing stem cell therapies using postnatal stem cells donated by the patients themselves or their close relatives. (bvsalud.org)

The sprouting cells had been subcultured in Dulbecco's revised eagle moderate (DMEM) including 920 mg/l D-valine 10 fetal bovine serum (FBS) 100 U/ml penicillin 100 g/ml streptomycin and 200 mM glutamine. (sciencepop.org)
Following the cells had been pass on and confluent for the … For all tests cultured rat penile soft muscle tissue cells (RPSMCs) had been expanded to 70 to 80% confluence and had been starved in DMEM without FBS every day and night. (sciencepop.org)
passages 5 Celecoxib to10) from 6 rats and cultured the cells in DMEM including 10% FBS 100 U/ml penicillin 100 μg/ml streptomycin and 200 mM glutamine. (sciencepop.org)

Thus, other factors related to the metabolic state of the cells, the microenvironment, or the formation of different drug products must account for the observed resistance. (researchgate.net)
This Module component contains an Agilent Seahorse XFp live cell metabolic analyzer. (utsouthwestern.edu)
Each individ- cluding immune cells, but also the was previously cal ed the gastroin- ual harbours hundreds of different metabolic and nervous systems), testinal flora or microflora, the more species, most of which have not and protecting against pathogens pragmatic term "microbiota" is now yet been cultured. (who.int)

We analyzed the system by cell viability, proliferation activity, RT-PCR and morphologic/immunohistoche-mical evaluations. (molcells.org)
The cell viability and proliferation activity were increased in time-dependent manner in our optimized HTS system. (molcells.org)
To achieve high-yield and viability neural cells from adult and aged macaques, we have incorporated knowledge accumulated from multiple fields. (springernature.com)

Here we develop and apply the principles of their evolution from systems principally for evaluating mechanism dosimetry in vitro and outline an approach for simulation of of toxicity to high-throughput systems for rapid and cost-effective nanoparticle particokinetics in cell culture systems. (cdc.gov)

In this work, we present a descriptive analysis of 16 commonly used ovarian cancer cell lines. (oncotarget.com)
The flow cytometer is equipped with multiple lasers for analysis of multi-fluorescent labelled cell populations and acoustical focusing for improved cell separations. (utsouthwestern.edu)
The Department of Ophthalmology owns two pieces of equipment that are useful for high throughput analysis. (utsouthwestern.edu)
3D cell culture, including the increased use of spheroids and organoids, presents unique cell imaging problems, especially when creating a digital model is necessary for experimental analysis. (corning.com)
Not only is the clearing process reversible, making 3D cultures available for further analysis, but the reagent does not alter cell morphology. (corning.com)
At Cambridge Healthtechs High Content Analysis conference, to be held in San Francisco later this month, companies will be presenting how their technology is making the bottom line a little thicker for major pharmaceutical companies. (genengnews.com)
The cost-benefit analysis of investment in the vector control programme in Brazil indicates that there are savings of US$17 in medical care and disabilities for each dollar spent on prevention, showing that the programme is a health investment with very high return. (scielo.br)
Images were analyzed using the 3D analysis module within the Custom Module Editor of MetaXpress® High- Content Image Acquisition and Analysis Software. (moleculardevices.com)
Here we describe a workflow for automation of organ-on chip culture, as well as monitoring, and automated cell analysis. (moleculardevices.com)

This enables the cells to fold up and create more three-dimensional types of structures which are about 200 microns in diameter," says Freedman. (corning.com)
The well geometry enables high-speed pipetting, excellent mixing and high-speed centrifugation to accelerate your manual as well as your automated work processes. (capitolscientific.com)

Here, we present a simplified metabolite extraction (SiMeEx) method, to efficiently and quickly extract metabolites from adherent mammalian cells. (frontiersin.org)
For adherent mammalian cells in culture the normal procedure involves their detachment from the surface. (frontiersin.org)
In this current study, we present a new and shorter method, termed as simplified metabolite extraction (SiMeEx), to extract metabolites from adherent mammalian cells. (frontiersin.org)

It helps to maintain optimal physiological conditions by minimizing photobleaching, providing high-performance imaging and high-throughput of data leading to better workflow efficiency and statistics. (news-medical.net)

Microfluidic cell culture integrates knowledge from biology, biochemistry, engineering, and physics to develop devices and techniques for culturing, maintaining, analyzing, and experimenting with cells at the microscale. (wikipedia.org)
For example, eukaryotic cells have linear dimensions between 10 and 100 μm which falls within the range of microfluidic dimensions. (wikipedia.org)
A key component of microfluidic cell culture is being able to mimic the cell microenvironment which includes soluble factors that regulate cell structure, function, behavior, and growth. (wikipedia.org)
Some of the major advantages of microfluidic cell culture include reduced sample volumes (especially important when using primary cells, which are often limited) and the flexibility to customize and study multiple microenvironments within the same device. (wikipedia.org)
Compartmentalized microfluidic cultures have also been combined with live cell calcium imaging, where depolarizing stimuli have been delivered to the peripheral terminals of neurons, and calcium responses recorded in the cell body. (wikipedia.org)
Particularly, microstructures inside microfluidic gadgets facilitate 3D cell tradition by guiding hydrogel precursors containing cells. (ncbcs.org)

A concentration gradient of fluorescence was observed from the outside to the centers of spheroids even after high concentrations (10 μ/ml) and long exposure times (2 hrs). (researchgate.net)

In this approach, researchers first collect antibody-producing cells from mice exposed to an antigen of interest and fuse them to myeloma cells, forming so-called hybridomas that can survive for a long time in culture. (the-scientist.com)
For each isolated cell, they separately clone the two genes encoding the heavy and light chains that compose an antibody, and that vary in sequence from cell to cell. (the-scientist.com)
This technique, commonly referred to as expression cloning, "is powerful because you're not limited-any B cell that you can isolate, in any context, you can make an antibody from," Wilson says. (the-scientist.com)
The issue with it is that there are a lot of steps for each cell to clone those antibody genes. (the-scientist.com)
It would be extremely laborious to use this strategy to try to identify an antibody against an antigen of interest using the blood of a person without an active immune response, he says, because millions of memory B cells are present in the blood, each of which is uniquely specific for a different antigen. (the-scientist.com)
CHARTING THE COURSE: B cells grow in culture in individual wells over a layer of feeder cells, and culture media from cells is screened for antibody-binding specificity or neutralizing activity. (the-scientist.com)
The immortalization of antibody-producing cells from patients infected with HIV can be inefficient, and even when cells are successfully immortalized, some clones tend to be unstable. (the-scientist.com)

The procedure to generate organoids uses a thin layer of Corning® Matrigel® matrix at the bottom of the tissue culture dish. (corning.com)

and integrated platforms to profile gene and protein expression and measure functional endpoints in cell cultures and on single cells. (nih.gov)

This resistance was not due to differences in the cell cycle state of these inner cells since separate experiments showed that both exponential and plateau phase monolayer cells were about equally sensitive when the surviving fraction was plotted vs absorbed drug (D0, = 0.04 μ/106 cells). (researchgate.net)
Additionally, researchers are concerned about the correct subtype stratification of the available cell line models, and the exploration of alternatives to monolayer culture. (oncotarget.com)

However, existing methods are lengthy, mostly due to the step of scraping cells from cell culture vessels, which restricts metabolomics in broader application such as lower cell numbers and high-throughput studies. (frontiersin.org)

Cells are embedded in hydrogels to simulate the in vivo extracellular matrix. (sigmaaldrich.com)
Both scaffolded and scaffold-free systems enable interaction in all directions with substrate, other cells, and extracellular factors. (sigmaaldrich.com)
Tissue engineering has developed many paradigms and techniques on how to best integrate cells and extracellular matrix to create in vitro structures that replicate native tissue. (biomedcentral.com)
The key elements of tissue engineering are stem cells, morphogen, and a scaffold of extracellular matrix. (bvsalud.org)

This protocol resulted in an increased number of NPCs and immature cells from the hippocampus, which revealed the primate-specific physiological properties of the neuron populations and the molecular cascade during adult neurogenesis. (springernature.com)
Watch Oksana Sirenko, Senior Application Scientist at Molecular Devices, present her poster on how our high-content imaging solutions can scale up and automate imaging of organ-on-a-chip systems. (moleculardevices.com)
Recently, the development of with food (in particular dietary fibre, comprising more than 100 trillion mi- molecular tools and subsequently of enabling energy harvest from oth- crobes living in the intestines, the next-generation sequencing enabled erwise indigestible dietary com- mouth, the skin, the vagina, and the richness of the intestinal ecosys- pounds), interacting with cells (in- elsewhere in the body. (who.int)

To further confirm the dissociation of NPCs from the hippocampus of adult macaques, we have undertaken studies on culture of NPCs from adult macaque hippocampus. (springernature.com)
Therefore, SiMeEx demonstrates advantages not only on time and resources, but also on the applicability in high-throughput studies. (frontiersin.org)
Numerous clinical studies have subsequently reported that individuals with CF in North America and Europe-while susceptible to severe COVID-19-are often spared from the highest levels of virus-associated mortality. (bvsalud.org)

Both of these latter techniques risk changes to the metabolism during cell preparation and barcoding prior to cell lysis- since both approaches, generally, rely on the use of live cells 30 . (nature.com)
Oxygen metabolism in the mitochondria contribute around 90% of ROS in a cell. (promocell.com)

We used colon carcinoma multicellular spheroids containing hypoxic and quiescent cells in core areas for this purpose. (researchgate.net)

We have stated the complex heterogeneity of ovarian cancer models, resembling primary tumors, agreeing with the argument that the cell line model for in vitro experiments must be carefully chosen. (oncotarget.com)
The Cell Phenotyping arm of this module is designed to provide technical expertise and assistance with ongoing cell phenotyping experiments. (utsouthwestern.edu)
The cell sorter has 4 lasers providing flexibility for experiments with up to 6 colors and with multiple sort collection formats. (utsouthwestern.edu)
A step-by-step guide for droplet-based single cell RNAseq experiments, practical considerations and technical notes. (rsc.org)
We next performed experiments to test the influence of homozygous CFTR deficiency on viral propagation and showed that SARS-CoV-2 production in primary airway cells was not altered by the absence of functional CFTR using two independent protocols. (bvsalud.org)

Human renal proximal tubule epithelial cells with over-expressed organic anion transporter 1 (RPTEC-OAT1) were exposed to serial dilutions of 5 AFFF (A-E), 5 SFFF (F-J), and 7 frequently detected PFAS in cell culture media for 6 - 24 hours. (cdc.gov)

THUNDER Imagers allow to visualize clearly in real time details even deep inside thick samples of biologically relevant models like model organisms, tissue sections, and 3D cell cultures. (news-medical.net)

Equipment in this laboratory includes an Attune NxT Flow Cytometer from Life Technologies and a Sony SH800 Cell Sorter. (utsouthwestern.edu)
The objective has been the same across all these technologies: more information on more cells at the lowest cost, balanced against the requirements for precision measurement and an attendant need for tailored bioinformatics 31 . (nature.com)
At the conclusion of treatment with test compounds, cells were stained with a mixture of two dyes: final concentration 1 μM calcein AM and 5 μM Hoechst 33342 (Life Technologies). (moleculardevices.com)
Then the cells were stained with 5 μM Hoechst 33342 and 0.06 μM AlexaFluor-488 Phalloidin (Life Technologies). (moleculardevices.com)
The lab led by Assistant Prof. Camila Consiglio employs high-throughput multiomics technologies and advanced computational methods to explore sex differences in human immunity. (lu.se)

Freedman was the first scientist in the western hemisphere to generate a kidney organoid from pluripotent stem cells. (corning.com)
The Matrigel matrix forms a crucial component of the kidney organoid generation process from pluripotent stem cells. (corning.com)
ES cells are pluripotent cells, which mean that they can give rise to all differentiated cell types derived from all three germ layers. (bvsalud.org)

Regenerative endodontic procedures can be defined as biologically based procedures designed to replace damaged structures, including dentin and root structures, as well as cells of the pulp-dentin complex. (bvsalud.org)
The major areas of research that might have application in the development of regenerative endodontic techniques are (a) postnatal stem cells, (b) scaffold materials, (c) morphogen/growth factors, (d) implantation. (bvsalud.org)
The most valuable cells for regenerative endodontics are postnatal or adult stem cells. (bvsalud.org)

Anatomy7

Organisms9

Diseases1

Chemicals and Drugs47

Analytical, Diagnostic and Therapeutic Techniques and Equipment99

Phenomena and Processes57

Disciplines and Occupations19

Anthropology, Education, Sociology and Social Phenomena2

Technology, Industry, Agriculture13

Information Science29

Health Care11

Assays24

Various high-throughput2

Flow Cytometry4

Microfluidics10

Screening26

Purification2

Isolation8

Quantitative3

Tissues10

Antibodies3

Yeast1

Differentiation4

MiRNA1

Adherent cells3

Compound2

Tumor4

Incubator1

Grown1

Gene expression3

Plates9

Recombinant2

Ovarian cancer cells6

Interactions3

Embryonic4

Characterization2

Tumour2

Receptors1

Genome1

Cytotoxicity2

Mesenchymal2

20221

Proteins3

Nuclei1

Spheroid6

Mass spectrometry1

Transcriptomic1

Scaffolds2

Molecules1

Reagents2

Stem cell8

DMEM3

Metabolic3

Viability3

Toxicity1

Analysis9

Enables2

Mammalian3

Physiological conditions1

Microfluidic6

Fluorescence1

Antibody7

Tissue culture1

Expression1

Monolayer2

Metabolomics1

Extracellular4

Molecular3

Studies3

Metabolism2

Colon carcinoma1

Experiments5

Epithelial cells1

Organisms1

Technologies5

Pluripotent3

Regenerative3