• High-density oligonucleotide microarrays were used to perform experiments for comparing data generated by using two protocols, an in vitro transcription (IVT) protocol that requires 5 μg of total RNA and a double in vitro transcription (dIVT) protocol that requires 200 ng of total RNA for target preparation from RNA samples extracted from a normal and a cancer cell line. (biomedcentral.com)
  • The 2 most common microarray technologies in use are the oligonucleotide microarrays and the robotically spotted complementary DNA (cDNA) microarrays. (medscape.com)
  • Oligonucleotide microarrays are manufactured by Affymetrix (Santa Clara, Calif) using photolithographic techniques. (medscape.com)
  • Antisense oligonucleotide (AO)-mediated exon skipping has been shown to restore functional dystrophin in mdx mice and DMD patients treated intramuscularly in two recent phase 1 clinical trials. (ox.ac.uk)
  • Antisense oligonucleotide (ASO) therapy has the potential to ameliorate many neurodegenerative diseases at the genetic level to suppress the production of harmful proteins or non-coding RNAs. (news-medical.net)
  • Interfering RNA and antisense oligonucleotide treatments currently available in France: an update]. (cdc.gov)
  • Therapeutic oligonucleotides continue to grow in their contribution to the pipelines of pharma and biopharma companies. (biopharminternational.com)
  • In this free webinar, Ajinomoto Bio-Pharma Services will discuss key steps for characterizing and overcoming the gaps between discovery and preclinical/clinical stage of oligonucleotide therapeutic development and manufacturing, as well as considerations for a smooth tech transfer. (prweb.com)
  • In collaboration with the Centre for Process Innovation (CPI), the two-year project will augment and validate the company's ML technology, Alchemite, as a tool to enhance productivity and yields of oligonucleotide therapeutic manufacture through performance prediction and optimisation of quality control strategies. (iptonline.com)
  • Offering high specificity with the ability to treat an extremely broad range of diseases, oligonucleotides represent a promising therapeutic option in pharmaceutical research. (iptonline.com)
  • Therapeutic oligonucleotides are large, complex molecules, requiring a multi-stage synthetic process alongside significant purification and analysis steps. (iptonline.com)
  • They are most commonly used as antisense oligonucleotides, small interfering RNA, primers for DNA sequencing and amplification, probes for detecting complementary DNA or RNA via molecular hybridization, tools for the targeted introduction of mutations and restriction sites, and for the synthesis of artificial genes. (wikipedia.org)
  • This qualitative in-vitro diagnostics test uses oligonucleotide probes labeled with four different fluorescent dyes. (cdc.gov)
  • Non-natural oligonucleotides are valuable probes of biological systems, as they can convey synthetic control of endogenous nucleic acids with high sequence specificity. (cdc.gov)
  • Besides, huge investments made by the pharmaceutical giants in oligonucleotide therapeutics for drug development are fostering the growth of the market. (medgadget.com)
  • Oligonucleotides are a chemically distinct class from small-molecule or protein therapeutics and present their own unique challenges for characterization and quantification. (biopharminternational.com)
  • TORONTO , Jan. 13, 2021 /PRNewswire-PRWeb/ -- A well-designed chemistry, manufacturing and control (CMC) strategy is essential when developing oligonucleotide therapeutics. (prweb.com)
  • Ben Pellegrini, CEO, Intellegens, said: "The potential of oligonucleotide therapeutics to treat a vast spectrum of diseases is an exciting prospect that is unfortunately held back by manufacturing capabilities. (iptonline.com)
  • Through CPI's Medicines Manufacturing Innovation Centre, we will look to bring together a community of experts from the pharmaceutical and technology companies to support the development of the Alchemite tools, reducing development times and improving manufacturability of oligonucleotide therapeutics. (iptonline.com)
  • To characterize identified variants and genes functionally, we rely on a broad repertoire of functional genomics methods , including CRISPR-Cas9, synthetic oligonucleotide libraries, and massively parallel reporter assays (MPRA). (lu.se)
  • We show that the use of single-stranded degenerate oligonucleotide primers consisting of a 10-15bp 3' tail, a 6-15bp degenerate interior, and a 2-6bp 5' anchor allow for random binding and amplification of pure or mixed DNA samples using two separate PCR cycling parameters. (aiche.org)
  • The ocular disposition and toxicity of GEM132, a mixed backbone phosphorothioate oligonucleotide developed for the treatment of cytomegalovirus-induced retinitis, were studied in rabbits for 6 months following single intravitreal injection of 5, 20, or 100 μg/eye (toxicity) and 3.7, 15.7, or 78.5 μg/eye (disposition). (aspetjournals.org)
  • Take advantage of our expertise and experience to build high quality research-grade oligos to your exact specifications with our large selection of probe chemistries, modifications, purification options and manufacturing scales. (biosearchtech.com)
  • Our extensive selection of nucleic acid derivatives and specialty building blocks is critical to our ability to synthesize high quality and structurally complex oligos. (biosearchtech.com)
  • Do you require large numbers of oligos for high-throughput applications? (biosearchtech.com)
  • You can rely on us to deliver high quality, bespoke oligos from small-scale RUO needs through the demands of commercial production. (biosearchtech.com)
  • Our ability to control these variables allows us to consistently produce high-quality oligos for use in routine and specialized applications. (idtdna.com)
  • Long, high-quality DNA oligos up to 200 bases. (idtdna.com)
  • DNA oligos up to 120 bases manufactured by an exclusive production process to minimize oligonucleotide crosstalk. (idtdna.com)
  • The synthesized oligos segment held the largest share of the market in 2022 and is expected to grow at the highest CAGR during the forecast period. (maximizemarketresearch.com)
  • The Asian market size is expected to grow at the highest CAGR, mainly owing to the rising accessibility of synthesized oligos, along with a rise in R&D funding and activities in the region. (maximizemarketresearch.com)
  • Thanks to its vast experience in the area of oligonucleotide synthesis (which extends beyond 30 years) as well as next generation sequencing (for more than 10 years), Microsynth knows exactly what the key requirements are for NGS oligos. (microsynth.com)
  • A novel, template-independent approach involving degenerated oligonucleotide primed PCR was used to generate genomic libraries reliably and rapidly from the genomic DNA of multiple bacteria with different methylation characteristics. (aiche.org)
  • For this, a functional genomic approach to embryogenesis has been developed that uses high-density oligonucleotide arrays for large-scale detection and quantification of gene expression. (unibas.ch)
  • Applications include separation of biotin-labeled biomolecules such as biotin- labeled antibodies, genomic DNA, RNA, PCR products, oligonucleotides (e.g. biotinylated oligo (dT) or peptides. (vectorlabs.com)
  • The oligonucleotide synthesis market is expected to register a CAGR of 12.3% over the forecast period. (mordorintelligence.com)
  • COVID-19 has impacted the growth of the oligonucleotide synthesis market. (mordorintelligence.com)
  • Oligonucleotide Synthesis Market was valued at US$ 5.75 Bn in 2022 and is expected to reach 15.20Bn by 2029, at a CAGR of 14.87% during the forecast period. (maximizemarketresearch.com)
  • The oligonucleotide synthesis market has found major development in the recent years and is anticipated to grow tremendously during the forecast period. (maximizemarketresearch.com)
  • The oligonucleotide synthesis market is expected to witness limitations in the availability of funding for research in academic laboratories, which may hamper market revenue growth. (maximizemarketresearch.com)
  • The report also helps in understanding Global Oligonucleotide Synthesis Market dynamics, structure by identifying and analyzing the market segments and project the global market size. (maximizemarketresearch.com)
  • 1. Global Oligonucleotide Synthesis Market analysis and forecast, in terms of value. (maximizemarketresearch.com)
  • Issues related to clinical validity include lack of standardization of assays used to measure Lp(a) levels and absence of universal guidelines for screening and subsequent risk assessment of high Lp(a) levels. (cdc.gov)
  • We also give guidelines for primer design and for the evaluation of assays using synthetic oligonucleotides. (lu.se)
  • In a study published in PLOS One in March 2022, it was observed that the oligonucleotides successfully targetted 5'-polyUs in the coronavirus genome. (mordorintelligence.com)
  • Considering the exponential traction, the market perceives currently Market Research Future (MRFR) in its recently published study report asserts that the global oligonucleotide pool market will reach USD 2.32 Billion by 2022, registering approximately 8.5% CAGR throughout the forecast period (2018 - 2022). (medgadget.com)
  • As a result, the European oligonucleotide pool market is expected to grow at 8.7% CAGR from 2018-2022. (medgadget.com)
  • Oligonucleotide pool is used in various research applications for designing and synthesizing of genes. (medgadget.com)
  • These oligonucleotide arrays were used for quantitative transcript imaging of embryonically expressed genes under standard conditions and in response to heat shock. (unibas.ch)
  • We analyzed twenty fecal samples taken from four premature infants (4-6 time points from each infant), and found significantly higher diametric ratios of genes associated with low oxygen levels in samples of infants later diagnosed with NEC than in samples without NEC. (osti.gov)
  • Bone mineralization and inflammation genes increase in muscles as a consequence of age and performance of a high repetition task. (cdc.gov)
  • These results show that a high repetition-low force reaching and grasping task induces genes related to both inflammation and bone mineralization in muscle tissues, suggesting that these molecules play a role in either inflammation or injury healing. (cdc.gov)
  • 1 In particular, morpholino oligonucleotides (MOs) have been used to block the expression of targeted genes in several invertebrate and vertebrate models. (cdc.gov)
  • Antisense mixed-backbone oligonucleotides (MBO) specifically inhibit MDM2 expression in a dose- and time-dependent manner, resulting in significant anti-tumor activity in vitro and in vivo. (nih.gov)
  • To overcome the issue, specifically designed PCR platform, STexS ( S NP t yping with ex cellent s pecificity) using double stranded oligonucleotides was implemented as a means to emphasize the amplification of mutated SNP templates by decreasing unwanted amplification of 3'mismatched DNA copies. (researchsquare.com)
  • We use our model to analyze a SELEX experiment containing double stranded DNA oligonucleotides and the transcription factor Bicoid as the target. (projecteuclid.org)
  • Products are often isolated by high-performance liquid chromatography (HPLC) to obtain the desired oligonucleotides in high purity. (wikipedia.org)
  • Microsynth has established and validated a specific process for the preparation of NGS oligonucleotides, based on a rigorous HPLC purification procedure in which the elimination of cross-contamination is a top priority. (microsynth.com)
  • For example, in the discovery stage, the priority would be high-throughput and purity of the target oligonucleotide, while in the clinical stage, the focus would be on prioritizing reliability, reproducibility and impurity control towards the commercial stage. (prweb.com)
  • For other salt forms or a guaranteed higher purity please inquire. (biolog.de)
  • Other significant factors include the increasing government investments and R&D expenditure in pharmaceutical and biotechnology companies and the use of synthesized oligonucleotides in molecular diagnostics and clinical applications. (mordorintelligence.com)
  • Additionally, the rising company and government initiatives to boost the development of oligonucleotide-based drug products and the adoption of oligonucleotide technology in the pharmaceutical and biotechnology industry are also expected to increase market growth. (mordorintelligence.com)
  • The biotechnology & Pharmaceutical Companies segment is expected to lead the market for oligonucleotide synthesis. (maximizemarketresearch.com)
  • mRNA was extracted using TRizol, Affymetrix oligonucleotide 230A genechip analysis performed and analyzed statistically (3-4/group). (cdc.gov)
  • The primary service of microbial arrays has been characterization of agents propagated to high titer in vitro ( 6 ). (cdc.gov)
  • These results demonstrate that high-density oligonucleotide arrays are sensitive, efficient, and quantitative instruments for the analysis of large scale gene expression in Drosophila embryos. (unibas.ch)
  • Methodologically, we combine large-scale genomics and high-throughput phenotyping with advanced computational and experimental approaches. (lu.se)
  • A brief introduction to high throughput technologies for measuring and analyzing gene expression is given. (lu.se)
  • We make use of high-resolution, high-throughput flow cytometry, advanced imaging techniques, gene expression data as well as crosslinking data from population based registries. (lu.se)
  • The increased demand for COVID-19 diagnostics has, in turn, increased the global demand for oligonucleotide raw materials that form the basis of molecular diagnostic products. (mordorintelligence.com)
  • The synthesizing of the oligonucleotide pool has significantly advanced scientific research, diagnostic development, and drug discovery. (medgadget.com)
  • We produce Diagnostic grade oligonucleotides under an ISO 13485 certified QMS. (eurogentec.com)
  • There is an urgent and unprecedented need for sensitive and high-throughput molecular diagnostic tests to combat the SARS-CoV-2 pandemic . (bvsalud.org)
  • LAMP showed higher diagnostic accuracy although DCDD was more cost-effective and did not require additional reagents or supplies. (who.int)
  • While the standard method, qPCR, allows for quick and cheap microRNA quantification, specificity is limited due to the short lengths of microRNAs and the high similarity between closely related microRNA family members. (lu.se)
  • In conclution, DNA amplification is a rapid, reliable and accurate method with a high degree of sensitivity and specificity for the detection of Mycobacterium tuberculosis DNA sequences and it can replace the conventional culture method in the diagnosis of extra pulmonary tuberculosis and tuberculosis meningitis except in the situation when antibiotic sensitivity results are required. (who.int)
  • Oligonucleotide pool allows the precise design and synthesis of thousands of user-defined sequences in parallel which can be utilized in high-throughput screening experiments for identification of novel gene mutations, optimization of protein structure, and function, or for drug discovery. (medgadget.com)
  • Most components in our manufacturing process are designed and developed in-house, including specialized synthesizers that accommodate the most demanding oligo requests and high-throughput automation systems that help to ensure fast turnaround times. (idtdna.com)
  • These advances are achieved without compromising the ultra-high sensitivity and extreme robustness for high-throughput proteomics, e.g., at 50 samples per day (SPD) or up to 200 SPD, or the high scientific confidence of better than 1% peptide and protein FDRs (false discovery rates), avoiding inherent antigen cross-reactivity in targeted immune-recognition methods. (bruker.com)
  • The timsTOF HT also is optimized for high-throughput, deep and unbiased plasma proteomics and liquid biopsy biomarker research. (bruker.com)
  • Data from a high throughput SELEX experiment consists of lists of thousands of oligonucleotides sampled after each round. (projecteuclid.org)
  • These beads are particularly suited for high throughput robotic applications where high biotin loads must be immobilized and separated using a suitably strong magnet. (vectorlabs.com)
  • NanoLINK streptavidin magnetic beads also provide an affordable alternative for automated, high throughput immobilization processes that utilize 96-well magnets to affect multiplex binding and separation of nucleic acid or immunoassay biomolecules. (vectorlabs.com)
  • InsertionMapper: a pipeline tool for the identification of targeted sequences from multidimensional high throughput sequencing data. (genomeweb.com)
  • This process is repeated to build specific oligonucleotide sequences. (medscape.com)
  • Here we present a generalized version of the RNA -mediated oligonucleotide Annealing Selection and Ligation with next generation DNA sequencing (RASL-seq) assay, called "capture RASL-seq" (cRASL-seq), which enables highly sensitive (down to ~1-100 pfu/ml or cfu/ml) and highly multiplexed (up to ~10,000 target sequences) detection of pathogens. (bvsalud.org)
  • For instance, in September 2021, SpeeDx invested in local oligonucleotide manufacturing to reduce supply chain risk and develop a more vital sovereign capacity for critical diagnostics. (mordorintelligence.com)
  • For instance, in February 2021, CPI launched a project in the Medicines Manufacturing Innovation Centre to revolutionize the manufacture of oligonucleotides. (mordorintelligence.com)
  • 20 different pathogens with high sensitivity, has proven useful for differential diagnoses of respiratory disease and viral hemorrhagic fevers ( 1 - 3 ). (cdc.gov)
  • In this webcast you will learn how high-resolution mass spectrometry (HRMS) and microflow LC-MS can help you overcome these challenges and still maintain a high level of sensitivity. (biopharminternational.com)
  • Recent developments in the market for oligonucleotide synthesis: In March 2018, Danaher Corporation publicized that they have acquired Integrated DNA Technologies. (maximizemarketresearch.com)
  • Pip5 transduction peptides direct high efficiency oligonucleotide-mediated dystrophin exon skipping in heart and phenotypic correction in mdx mice. (ox.ac.uk)
  • Furthermore, the increasing adoption of oligonucleotides in molecular diagnostics and clinical applications contributes to market growth. (mordorintelligence.com)
  • These results provide a basis for clinical evaluation of antisense anti-MDM2 oligonucleotides as chemosensitizers and radiosensitizers. (nih.gov)
  • Methods for direct molecular detection of microbial pathogens in clinical specimens are rapid, sensitive, and may succeed when fastidious requirements for agent replication or the need for high-level biocontainment confound cultivation. (cdc.gov)
  • However, sponsors typically change oligonucleotide partners when moving from the research and discovery stage to preclinical/clinical stage as well as to the commercial stage. (prweb.com)
  • The context of discriminating a mutant within a clinical sample has to have high specificities which can detect 1% or less included mutated DNA alongside a high robustness for further clinical implementations. (researchsquare.com)
  • Tg2576 mice overexpress a mutant form of human amyloid precursor protein with the Swedish mutation (APP Sw ), resulting in high β-amyloid (Aβ) levels in the brain. (jneurosci.org)
  • Following this methodology, sets of tri- and tetradeoxyribonucleotides were synthesized and were enzymatically converted to longer oligonucleotides, which allowed elucidation of the genetic code. (wikipedia.org)
  • Construction of High-Density Genetic Map and Mapping Quantitative Trait Loci for Growth Habit-Related Traits of Peanut (Arachis hypogaea L. (frontiersin.org)
  • A new high-density genetic linkage map was constructed by using specific locus amplified fragment sequencing technology. (frontiersin.org)
  • Mendelian Randomization (MR) studies which are less subject to confounding and reverse causation, have assessed genetic variants affecting Lp(a) levels and provided evidence for direct causal relationships between high Lp(a) and CVD . (cdc.gov)
  • Our data analysis work involves mathematical modeling , and high-performance computing and we develop computational methods for large scale genetic data. (lu.se)
  • North America is expected to hold the largest market size for the oligonucleotide synthesis globally. (maximizemarketresearch.com)
  • Thus, microbes can be detected when melting temperatures are high enough to allow hybridization, despite a lack of precise complementarity between probe and target. (cdc.gov)
  • This approach involves the intramolecular cross-linking of 5′ amine- and 3′ disulfide-modified MO oligonucleotides using appropriately functionalized tethers, generating macrocyclic structures that conformationally resist RNA hybridization. (cdc.gov)
  • Although the recognition of CpG DNA by B cells and plasmacytoid dendritic cells is mediated by TLR 9, these cell types differ in their ability to bind and respond to structurally distinct classes of CpG oligonucleotides. (fda.gov)
  • The Medicines Manufacturing Innovation Centre launched a collaboration with AstraZeneca, Exactmer, Novartis, and UK Research and Innovation to develop a scalable, sustainable, and more cost-effective medicines manufacturing process for oligonucleotides: short strands of synthetic DNA or RNA. (mordorintelligence.com)
  • There was a significantly higher knowledge about Fatwa on smoking among men than women. (who.int)
  • The ion occupancies derived from the MRD data are substantially higher than previously reported, suggesting that groove-bound ions significantly influence the energetics and structural polymorphism of DNA in vivo. (lu.se)
  • Reducing the prevalence of HPV infection is a Developmental Healthy People 2010 objective: "Reducing the number of new HPV cases can help minimize the overall number of cases of high risk subtypes associated with cervical cancer in females. (cdc.gov)
  • 30 August 2023 -- Cambridge, UK -- Intellegens, a company applying machine learning (ML) to accelerate innovation in R&D, announced that it has secured a £1.6m grant from Innovate UK, part of UK Research and Innovation (UKRI), to apply ML in the emerging field of oligonucleotide therapies. (iptonline.com)
  • Oligonucleotides are complex molecules and the process of bringing them to market is costly, meaning very few companies are able to harness this potentially life-saving and life-changing technology. (iptonline.com)
  • These DNA fragments clone at high efficiency to commercial TOPO™ cloning vectors and are easily transposed into other vectors using the Gateway™ cassette system. (aiche.org)
  • The continuous effort to improve the overall mixture was mainly aimed to enhance reactions, diminish unnecessary primer dimers, reduce nonspecific binds due to mismatches, and block efficiency drops occurred by high G/C rates [6, 7]. (researchsquare.com)
  • The kit incorporates unique D-Plex technology for library preparation enabling high performance with ultralow RNA inputs and providing diverse small RNA transcript detection. (mordorintelligence.com)
  • Highly multiplexed oligonucleotide probe-ligation testing enables efficient extraction-free SARS-CoV-2 detection and viral genotyping. (bvsalud.org)
  • Our simplified protocol additionally enables the direct and efficient genotyping of selected, informative SARS-CoV-2 polymorphisms across the entire genome , which can be used for enhanced characterization of transmission chains at population scale and detection of viral clades with higher or lower virulence . (bvsalud.org)
  • We first showed that fluorescent signal from the oligonucleotide array varies in proportion to both decreases and increases in copy number. (ox.ac.uk)
  • Oligonucleotides find a variety of applications in molecular biology and medicine. (wikipedia.org)
  • Additional factors boosting the market growth include the growing complimentary industries, augmented adoption of precision medicine, and the increasing applications of oligonucleotide pool in biotechnology and molecular biology. (medgadget.com)
  • The evolution of oligonucleotide synthesis saw four major methods of the formation of internucleosidic linkages and has been reviewed in the literature in great detail. (wikipedia.org)
  • In the early 1950s, Alexander Todd's group pioneered H-phosphonate and phosphate triester methods of oligonucleotide synthesis. (wikipedia.org)
  • Various supervised and unsupervised data mining methods for analyzing the produced high- dimensional data are discussed. (lu.se)
  • This webcast will seek to provide you with a high-resolution mass spectrometry (HRMS) workflow to identify and characterize these impurities. (biopharminternational.com)
  • The Mass Spectrometry Senior Scientist - Biochemistry position at Eurofins Scientific involves performing method development, qualification, and validation using High Resolution Mass Spectrometry for. (theladders.com)
  • Vaginal swab samples were processed, stored, and shipped to the Chronic Viral Diseases Branch, Division of High-Consequence Pathogens and Pathology, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA for analysis. (cdc.gov)
  • However, the technique to discriminate small amounts of SNP mixed in abundant normal DNA is incomplete due to intrinsic technical problems of PCR such as amplification occurring even in 3'mismatched cases because of high enzyme activity of DNA polymerases. (researchsquare.com)
  • Such research studies have impacted the demand and adoption for oligonucleotides in drug development, affecting the market growth during a pandemic. (mordorintelligence.com)
  • Such developments are expected to increase the adoption of oligonucleotides in drug development which is anticipated to fuel the market growth over the forecast period. (mordorintelligence.com)
  • This is expected to increase the demand for oligonucleotides for drug development, boosting the market growth. (mordorintelligence.com)
  • On the flipside, the nascent stage of the oligonucleotide pool is a considerable headwind that is posing some challenges to the market growth such as the lack of funds for R&D and the high cost of development. (medgadget.com)
  • The North American region dominates the global oligonucleotide pool market owing to the presence of leading global players and encouraging governmental support for the research & development activities in healthcare. (medgadget.com)
  • As a result, these companies are making major use of oligonucleotides, as it offers an effective mode of action for the treatment of several indications at comparatively lower development costs. (maximizemarketresearch.com)
  • Oligonucleotide development and manufacturing requires many particular capabilities, from specialized manufacturing centers and equipment to process and analytical methodology development, as well as technical expertise. (prweb.com)
  • For this reason, contract development and manufacturering organizations (CDMO), who specialize in oligonucleotides, play a crucial role in supporting a client's program from early drug development to commercial manufacturing. (prweb.com)
  • Learn from Ajinomoto Bio-Pharma Services' more than 20 years of focused experience and application in oligonucleotide development and manufacturing. (prweb.com)
  • The collaboration will validate Alchemite as a tool to improve productivity in oligonucleotide process development and manufacturing workflows. (iptonline.com)
  • We have evaluated the effectiveness of an oligonucleotide array, originally designed to detect single-nucleotide polymorphisms, to assess DNA copy number. (ox.ac.uk)
  • They want to provide high-quality products and services to the scientist around the world. (maximizemarketresearch.com)
  • To provide support to CDC laboratories in responding to public health needs through the supply of state-of-the art technology and high-quality services and products. (cdc.gov)
  • Provide CDC laboratorians with high-quality laboratory products and services in compliance with US Food and Drug Administration regulations. (cdc.gov)
  • Oligonucleotides synthesis takes place technologically to give services to different end-users or independently by means of DNA synthesizers for self-utilization. (maximizemarketresearch.com)
  • These mice, therefore, may not be a good model of the complete pathologic process of AD but rather may be an excellent model for understanding how the brain can adapt to and survive high levels of Aβ. (jneurosci.org)
  • The burden of gastrointestinal diseases and disorders (GIDD) remains high in Nigeria and varies across regions. (bvsalud.org)
  • The method is very convenient in that various types of phosphate modifications (phosphate/phosphorothioate/phosphoramidate) may be introduced to the same oligonucleotide for modulation of its properties. (wikipedia.org)
  • Several MO caging strategies have been devised, employing hairpin structures, 7 intermolecular duplexes, 8 , 9 nucleobase modifications, 10 or oligonucleotide cyclization. (cdc.gov)
  • Thus far, SELEX experiments have been very good at suggesting the highest affinity oligonucleotide, but modeling lower affinity recognition site variants has been difficult. (projecteuclid.org)
  • Considering the complexity of MDM2 functions, we have chosen to use gene silencing technologies including antisense oligonucleotides and RNA interference. (nih.gov)
  • The technique is extremely useful in current laboratory practice because it provides a rapid and inexpensive access to custom-made oligonucleotides of the desired sequence. (wikipedia.org)
  • Herein, we report on a specific oligonucleotide-based RNA inhibitor targeting severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). (mdpi.com)
  • One recommendation has been to establish ancestry-specific cut points to define a high Lp(a) level. (cdc.gov)
  • Genome-wide association studies (GWAS) , observational cohort studies , and meta-analyses of prospective, population-based studies provide evidence supporting a causal relationship between high Lp(a) and ASCVD, aortic stenosis, and stroke. (cdc.gov)
  • The inventors' work established that CXCL16, a membranebound scavenger receptor, influences the uptake, subcellular localization, and cytokine profile induced by D oligonucleotides. (fda.gov)