• vitro
  • Joplin R, Hishida T, Tsubouchi H, et al Human intrahepatic biliary epithelial cells proliferate in vitro in response to human hepatocyte growth factor. (springer.com)
  • EGCG in concentrations lower than 10 μ mol/L was recognized as safe for hepatocytes in vitro . (hindawi.com)
  • To date, primary human hepatocytes have been considered the gold standard for this evaluation as they provide a model to assess key criteria such as drug transport, metabolism, clearance, and in vitro toxicity. (clontech.com)
  • Moreover, large batches of primary human hepatocytes can be unreliable: they can display variable quality, show large donor variation, and lose functionality very rapidly-even within a few days of in vitro culture. (clontech.com)
  • Moreover, in order to be useful, iPS-derived hepatocytes should recapitulate the diversity of metabolic phenotypes observed in human populations (3), express drug metabolizing enzymes at equivalent levels to primary human hepatocytes, and maintain stable functionality in vitro (4). (clontech.com)
  • In vitro model systems based on hepatocytes have been of great help to better understand the role of hepatocytes in (patho)physiological processes of the liver. (wikipedia.org)
  • Li and his researchers have developed numerous hepatocyte-based assays, including higher throughput assays for P450 inhibition, time-dependent inhibition, P450 induction, cytokine suppression of ADME gene expression, and in vitro hepatotoxicity. (wikipedia.org)
  • nuclei
  • Hepatocyte nuclei are round with dispersed chromatin and prominent nucleoli. (wikipedia.org)
  • Anisokaryosis (or variation in the size of the nuclei) is common and often reflects tetraploidy and other degrees of polyploidy, a normal feature of 30-40% of hepatocytes in the adult human liver. (wikipedia.org)
  • Human
  • A broad-spectrum human lung fibroblast-derived mitogen is a variant of hepatocyte growth factor. (springer.com)
  • Molecular cloning and sequence analysis of cDNA for human hepatocyte growth factor. (springer.com)
  • Molecular cloning and expression of human hepatocyte growth factor. (springer.com)
  • Our rat hepatocytes are prepared using the same careful isolation and cryopreservation techniques as our human lots. (thermofisher.com)
  • Human hepatocytes are highly attractive candidates for cell-based therapy for chronic liver disease, and are essential to many studies in areas such as disease modeling, hepatitis research, and alcoholic fatty liver disease. (clontech.com)
  • However, the use of human hepatocytes is limited by their lack of availability and large inter-individual variations (1). (clontech.com)
  • This method offers the unique possibility of an unlimited source of hepatocytes from various human donors. (clontech.com)
  • For these purposes, hepatocytes are usually isolated from animal or human whole liver or liver tissue by collagenase digestion, which is a two-step process. (wikipedia.org)
  • The following is a list of human hepatocyte nuclear factors (see also boxes to the right for additional information about these proteins): Members of the HNF1 subfamily contain a POU-homeodomain and bind to DNA as homodimers. (wikipedia.org)
  • After establishing IVAL, Li was the first to report successful cryopreservation of human hepatocytes to retain their viability, functions, and ability to be cultured (plateable cryopreserved human hepatocytes). (wikipedia.org)
  • protein
  • 2-Cyanoethylene oxide accumulated in the hepatocyte incubations but did not appear to contribute extensively to alkylation of GSH or protein. (aacrjournals.org)
  • The ability of nonprotein thiols to modulate rates of protein synthesis was investigated in isolated rat hepatocytes. (springer.com)
  • Time- and dose-dependent protein synthesis inhibition takes place following exposure to high doses of dimethylnitrosamine (DMN) or diethylnitrosamine (DENA) in isolated rat hepatocytes. (springer.com)
  • Hepatocyte growth factor has been shown to interact with the protein product of the c-Met oncogene, identified as the HGF receptor (HGFR). (wikipedia.org)
  • Both overexpression of the Met/HGFR receptor protein and autocrine activation of Met/HGFR by simultaneous expression of the hepatocyte growth factor ligand have been implicated in oncogenesis. (wikipedia.org)
  • Metabolism
  • Rooda S.J.E., van Loon M.A.C., Visser T.J. (1986) Metabolism of rT 3 by Isolated Rat Hepatocytes. (springer.com)
  • In order to establish the relative contributions of the various pathways leading to activated and detoxicated products, we examined AN metabolism in isolated Fischer 344 rat hepatocytes as a model. (aacrjournals.org)
  • Hepatocytes have the ability to metabolize, detoxify, and inactivate exogenous compounds such as drugs, (drug metabolism), and insecticides, and endogenous compounds such as steroids. (wikipedia.org)
  • In addition, pharmaceutical industry has heavily relied on the use of hepatocytes in suspension or culture to explore mechanisms of drug metabolism and even predict in vivo drug metabolism. (wikipedia.org)
  • isolation
  • Get Alerts and Information When New Hepatocyte Isolation or Lots Are aAvailable. (corning.com)
  • HepatoLink® is the Corning immediate response system providing researchers with instant notification when new hepatocyte isolation events occur. (corning.com)
  • viability
  • To maximize post-thaw viability and recovery of Corning cyropreserved hepatocytes, a cryohepatocyte recovery kit was developed (Cat. (corning.com)
  • Albumin
  • The hepatocyte is a cell in the body that manufactures serum albumin, fibrinogen, and the prothrombin group of clotting factors (except for Factors 3 and 4). (wikipedia.org)
  • exhibit
  • However, current hepatocyte differentiation protocols exhibit varying differentiation efficiencies across diverse iPS cell lines derived from multiple donor sources (2). (clontech.com)
  • sinusoids
  • Hepatocytes are organised into plates separated by vascular channels (sinusoids), an arrangement supported by a reticulin (collagen type III) network. (wikipedia.org)
  • These surfaces are the place where the exchange of substances between the hepatocytes and the sinusoids occurs. (wikipedia.org)
  • The intercellular surfaces are the ones that are between two adjacent hepatocytes and they are not in contact with sinusoids or canaliculi. (wikipedia.org)