Foot-and-mouth disease viruses (FMDVs) target epithelial cells via integrin receptors, but can acquire the capacity to bind cell-surface heparan sulphate (or alternative receptors) on passage in cell culture. (ox.ac.uk)
Heparan sulfate proteoglycans2
The high level of expression of PECAM-1 on CD34+ cells suggests that this glycoprotein may function as a heterotypic adhesion molecule, possibly mediating multipotential, myeloid, and early-B-lymphoid precursor cell interactions with stromal cells and extracellular matrix molecules via heparan sulfate proteoglycans. (ox.ac.uk)
The systematic name of this enzyme class is heparin-sulfate lyase. (wikipedia.org)
Other names in common use include heparin-sulfate eliminase, heparitin-sulfate lyase, heparitinase I, and heparitinase II. (wikipedia.org)
Sequence1
This virus has an RGSD motif in place of the otherwise conserved RGD integrin-binding motif and the potential to bind heparan sulphate (suggested by sequence analyses). (ox.ac.uk)
Bind1
A study of the direct interaction between AAV2 and artificial glycosaminoglycans showed that a high degree of sulfation on heparin was critical for the ability to bind AAV2 and compete rAAV2 transduction and that both O- and N-sulfate groups are required. (ox.ac.uk)
Receptor2
Recently heparan sulfate was proposed as the host cell receptor for the dependovirus, adeno-associated virus type 2 (AAV2). (ox.ac.uk)
Overall, our data suggest that, as has been shown for other viruses, the presence of a high-affinity AAV2 receptor mediates AAV2 infection in addition to the low-affinity heparan sulfate binding. (ox.ac.uk)
Cells4
We show that although heparan sulfate on the cell surface may contribute to the binding of AAV2 to permissive cells, the amount of heparan sulfate on the cell surface as determined by flow cytometry using four different monoclonal antibodies does not correlate with AAV2 binding to cells or recombinant AAV2 transduction efficiency. (ox.ac.uk)
Experiments with either mutant CHO cells or cells treated with chlorate to remove sulfate groups showed that sulfation was not absolutely required for infection or binding: in the absence of cell surface sulfation, recombinant AAV2 was still able to be transduced in previously permissive cells. (ox.ac.uk)
The heparin binding PECAM-1 adhesion molecule is expressed by CD34+ hematopoietic precursor cells with early myeloid and B-lymphoid cell phenotypes. (ox.ac.uk)
Hypoxia triggers a proangiogenic pathway involving cancer cell microvesicles and PAR-2-mediated heparin-binding EGF signaling in endothelial cells. (lu.se)
Cell surface1
These data suggest that heparin, heparan, and lipoproteins may play an important in vivo role in regulating cell surface associated activation of the fibrinolytic system. (duke.edu)
Virus1
The interaction of heparin sulfate and adeno-associated virus 2. (ox.ac.uk)
Publication1
Scholars@Duke publication: Kinetic analysis of the effects of heparin and lipoproteins on tissue plasminogen activator mediated plasminogen activation. (duke.edu)
Structure1
The structure of FMDV A10(61) (a cell culture-adapted strain) complexed with heparin has now been determined. (ox.ac.uk)
Studies3
Heparin is commonly used as a substitute in studies of the interaction between heparan sulfate and ligand, and we demonstrate that the binding affinity of AAV2/heparin is low, with a K(d) value of approximately 2.0 nM. (ox.ac.uk)
Kinetic studies indicate that both heparin and heparan increase the kcat of t-PA-mediated Pg activation by 25- and 3.5-fold, respectively. (duke.edu)
Preliminary studies show that a proportion of the PECAM-1 molecules on the lymphomyeloid/multipotential progenitor cell line, KG1, and on the monocytic cell line, U937, binds to heparin-sepharose. (ox.ac.uk)
Activity1
Both heparin and heparan stimulate the activity of t-PA by interacting with the finger domain of t-PA, with association constants of 1 microM and 200 nM, respectively. (duke.edu)
Type2
Heparin sulfate and the less sulfated glycosaminoglycan heparan sulfate enhance human plasminogen (Pg) conversion to plasmin by tissue-type plasminogen activator (t-PA). (duke.edu)
Crystal structures are available for type O, A and C viruses and also for a complex of type O strain O(1)BFS with heparin. (ox.ac.uk)
Compelling evidence suggests that both heparan sulfate (HS) and chondroitin sulfate (CS) glycosaminoglycans (GAGs) bind to a series of Lys residues located in the first Ig domain of RPTPσ. (nih.gov)
Proteoglycans consisting of proteins linked to one or more CHONDROITIN SULFATE-containing oligosaccharide chains. (lookformedical.com)
Enzymes which catalyze the elimination of glucuronate residues from chondroitin A,B, and C or which catalyze the hydrolysis of sulfate groups of the 2-acetamido-2-deoxy-D-galactose 6-sulfate units of chondroitin sulfate. (lookformedical.com)
In this work, we isolated two new sulfated glycans from the body wall of the sea cucumber Thyonella gemmata: one fucosylated chondroitin sulfate (TgFucCS) (17.5 ± 3.5% kDa) and one sulfated fucan (TgSF) (383.3 ± 2.1% kDa). (bvsalud.org)
Glycosaminoglycan3
In our body's cells, Heparan Sulfate Proteoglycans (Proteoheparan Sulfate) , as ubiquitous proteoglycan macromolecules , consisting of a core protein to which, the glycosaminoglycan heparan sulfate (heparitin sulfate) chains, are attached and held, associated with the cell surface and extracellular matrix of a wide range of tissue cells. (wellnessadvocate.com)
Glycosaminoglycan (GAG) sequences that selectively target heparin cofactor II (HCII), a key serpin present in human plasma, remain unknown. (nih.gov)
The presence of the glycosaminoglycan side chains of heparan sulfate proteoglycan, an important constituent of the glomerular basement membrane, is decreased in DKD proportionally to the increasing degree of proteinuria. (cochrane.org)
Eliminase1
Other names in common use include heparin-sulfate eliminase, heparitin-sulfate lyase, heparitinase I, and heparitinase II. (wikipedia.org)
Heparan sulphate3
Sanfilippo syndrome occurs when enzymes that are needed to break down the heparan sulphate sugar chain are either defective or missing entirely. (syndromespedia.com)
Foot-and-mouth disease viruses (FMDVs) target epithelial cells via integrin receptors, but can acquire the capacity to bind cell-surface heparan sulphate (or alternative receptors) on passage in cell culture. (ox.ac.uk)
This virus has an RGSD motif in place of the otherwise conserved RGD integrin-binding motif and the potential to bind heparan sulphate (suggested by sequence analyses). (ox.ac.uk)
Proteoglycans2
In our body's cells, Heparan Sulfate Proteoglycans (HSPG) are essential cofactors in cell-matrix adhesion processes, in cell-cell recognition systems, and in receptor-growth factor interactions. (wellnessadvocate.com)
"Heparanases: endoglycosidases that degrade heparan sulfate proteoglycans" Glycobiology. (wellnessadvocate.com)
Accumulates1
In our body, Heparitin Sulfate (Heparan Sulfate) is a heteropolysaccharide that accumulates in individuals with the dis-ease process mucopolysaccharidosis . (wellnessadvocate.com)
Using a computational strategy on a library of 46 656 heparan sulfate hexasaccharides we identified a rare sequence consisting of consecutive glucuronic acid 2-O-sulfate residues as selectively targeting HCII. (nih.gov)
Interactions1
The relevance of heparin and HS biochemical interactions was demonstrated by competition of RSP and DV binding to cells with soluble heparin and HS. (nih.gov)
Body1
The children who afflict with Sanfilippo Syndrome miss an essential enzyme that breaks down strings of complex body sugar called heparin sulphate. (syndromespedia.com)
Similar1
Heparin bound at a similar site and in a similar conformation to that seen in the analogous complex with O(1)BFS, although the binding had a lower affinity and was more ionic. (ox.ac.uk)
Library1
A library of HS oligomers was used to verify the HS binding activity of hMPV F, and several compounds showed binding to predominantly prefusion hMPV F, but had limited binding to postfusion hMPV F. Furthermore, MAbs to antigenic sites III and the 66-87 intratrimeric epitope block heparin binding. (edu.au)