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Electrophoresis, Cellulose AcetateHemoglobinopathiesSickle Cell TraitBlood Protein ElectrophoresisCelluloseHemoglobinsHemoglobins, AbnormalElectrophoresisHemoglobin AAcetatesHemoglobin CHemoglobin, SickleNeisseriaElectrophoresis, Polyacrylamide GelNeisseria siccaMembranes, ArtificialFetal HemoglobinCellulaseElectrophoresis, CapillaryElectrophoresis, Agar GelCellulose 1,4-beta-CellobiosidaseMolecular WeightCellulose, OxidizedElectrophoresis, Gel, Two-DimensionalFiltrationGlycosaminoglycansIsoelectric FocusingChromatography, Ion ExchangeElectrophoresis, Gel, Pulsed-FieldDEAE-CelluloseCrown EthersChromatography, DEAE-CelluloseTetradecanoylphorbol AcetateTolonium ChlorideHydrogen-Ion ConcentrationOxyhemoglobinsImmunoelectrophoresisHemoglobin EHemoglobin A, GlycosylatedHemoglobin A2Tablets, Enteric-CoatedMethylcelluloseIsoenzymesChromatography, GelGluconacetobacter xylinusKineticsExcipientsAcetic AcidAmino AcidsDermatan SulfatePolymersUronic AcidsDensitometrySolubilityEsterasesMethemoglobinMethodsChemistry, PharmaceuticalCarboxymethylcellulose SodiumMolecular Sequence DataTabletsTruncated HemoglobinsElectrophoresis, MicrochipAlkaline PhosphataseTemperatureUltracentrifugationImmunodiffusionAnemiaCarboxylic Ester HydrolasesElectrophoresis, Starch GelElectrophoresis, DiscHydrolysisChondroitin SulfatesCarboxyhemoglobinHemoglobinometryAcetate KinaseChondroitinBlood ProteinsCellobioseCellulasesBiodegradation, EnvironmentalGlucosyltransferasesOxygenSubstrate SpecificityErythrocytesSerum AlbuminHyaluronic AcidAmino Acid SequenceMicroscopy, Electron, ScanningWaterLiverSodium AcetateHemoglobin JTime FactorsTrichodermaHemoglobin C DiseaseEvaluation Studies as TopicBlood SubstitutesRabbitsHemoglobin H
Paper ElectrophoresisFetal hemoglobinStarch gel electrophoresisCapillaryCitrate agarPolyacrylamide gelAbnormal hemoglobinPeripheral bloodAgarosePathophysiologyMoleculeHemoglobinopathiesClinicallyTypes of hemoglobinAmount of hemoglobinVariantsMembraneAdultProteinSickle cellAlkalineClinicalDetectSubunitsSeparationMilderTraitChainsDiagnosisMigrationClassificationDecreasesAmountsSuitableGenesDistinguishBloodMethodUnitConsequencesMinorAffinityOxygenSymptomsEvaluationHumanProductionResultsBetaConditionsDiseaseTypeTest
Paper Electrophoresis4
Fetal hemoglobin6
Starch gel electrophoresis3
Capillary4
Citrate agar2
Polyacrylamide gel1
  • Electrophoresis in which a polyacrylamide gel is used as the diffusion medium. (lookformedical.com)
Abnormal hemoglobin3
  • and a minute amount of hemoglobin F.: 140 If abnormal hemoglobin variants such as hemoglobin S (which occurs in sickle cell disease), C or E are present, they will appear as unexpected bands on electrophoresis (provided they do not migrate to the same place as other hemoglobins). (wikipedia.org)
  • Hemoglobin's electrophoresis showed the presence of an abnormal hemoglobin ( Fig. 2 ) which was subsequently documented to be HbS. (revistanefrologia.com)
  • Hemoglobin S was the first abnormal hemoglobin to be identified. (msdmanuals.com)
Peripheral blood1
  • The initial laboratory workup of inherited disorders of hemoglobin should include a complete blood count (CBC) and peripheral blood smear, followed by carefully selected tests based on clinical suspicion and available methodology. (bvsalud.org)
Agarose4
Pathophysiology2
  • Here, we review the molecular pathophysiology of hemoglobin disorders and review both old and new methods of diagnosing these disorders. (bvsalud.org)
  • A comprehensive understanding of the molecular pathophysiology of hemoglobin and the globin genes, as well as a clear understanding of the utility and limitations of currently available diagnostic tests, is essential in reducing global disease burden. (bvsalud.org)
Molecule4
  • Hemoglobin electrophoresis can also be used to investigate thalassemias, which are caused by decreased production of subunits of the hemoglobin molecule. (wikipedia.org)
  • Hemoglobinopathies are genetic disorders that affect the hemoglobin (Hb) molecule. (bvsalud.org)
  • Загальні відомості про гемоглобінопатії Hemoglobinopathies are genetic disorders affecting the structure or production of the hemoglobin molecule. (msdmanuals.com)
  • The normal adult hemoglobin molecule (Hb A) consists of 2 pairs of chains designated alpha and beta. (msdmanuals.com)
Hemoglobinopathies1
Clinically4
  • Persons with hemoglobin C trait (Hb AC) are phenotypically normal, with no clinically evident symptoms, while those with hemoglobin C disease (Hb CC) may have a mild degree of hemolytic anemia and sequelae of hemolysis including jaundice, gallstones, or splenomegaly. (medscape.com)
  • Hemoglobin C trait (Hb AC) is clinically silent. (medscape.com)
  • Mass spectrometry, a newer analytical technology, may be used to identify highly unstable hemoglobins that may manifest clinically as hemolytic anemia or thalassemia. (medscape.com)
  • The genetic regulation of hemoglobin is complex and there are a number of genetic abnormalities that result in clinically important hemoglobin disorders. (bvsalud.org)
Types of hemoglobin3
  • Hemoglobin electrophoresis is a blood test that can detect different types of hemoglobin. (wikipedia.org)
  • The test can detect hemoglobin S, the form associated with sickle cell disease, as well as other abnormal types of hemoglobin, such as hemoglobin C. It can also be used to investigate thalassemias, which are disorders caused by defective hemoglobin production. (wikipedia.org)
  • The test uses the principles of gel electrophoresis to separate out the various types of hemoglobin and is a type of native gel electrophoresis. (wikipedia.org)
Amount of hemoglobin1
  • Therefore, they also don't have enough room for the average amount of hemoglobin. (mastelf.com)
Variants2
  • Cellulose acetate (CA) electrophoresis at pH 8.2-8.6 can be used to resolve common variants such as HbS and HbC, but it cannot be used to distinguish between HbS and HbD Punjab and HbG Philadelphia . (medscape.com)
  • There are 1,424 variants of human hemoglobin described with 951 involving the ß-globin gene. (bvsalud.org)
Membrane2
  • 2011. Haemoglobin electrophoresis was performed on cellulose acetate membrane, followed by staining and densitometric scanning of bands. (who.int)
  • Une électrophorèse de l'hémoglobine a été réalisée sur une membrane d'acétate de cellulose, suivie par une coloration puis une analyse densitométrique des bandes. (who.int)
Adult3
  • This means that the fetal hemoglobn has a higher affinity for oxygen than the adult hemoglobin and transfer of oxygen from the low affinity HbA to the higher affinity HbF is thus enabled. (flashcardmachine.com)
  • Normal adult hemoglobin (HbA) has two α- and two β-globin chains (α 2 β 2 ). (medscape.com)
  • Minor adult hemoglobin (HbA 2 ) is made of two α- and two δ-globin chains (α 2 δ 2 ). (medscape.com)
Protein3
  • What type of protein-protein interaction is disrupted in hemoglobin when oxygen binds? (flashcardmachine.com)
  • Examined by the characteristics of the protein standard on cellulose acetate tape and pH 8.6 barbital buffer. (eceratrading.com)
  • In Protein Electrophoresis: technologists and authors. (democo.de)
Sickle cell2
  • What mutation in hemoglobin causes sickle cell? (flashcardmachine.com)
  • Recognizing that most of the global burden of hemoglobin disorders exists in low- and middle-income countries, we review the increasingly available array of point-of-care-tests (POCT), which have an increasingly important role in expanding early diagnosis programs to address the global burden of sickle cell disease, including Sickle SCAN, HemoTypeSC, Gazelle Hb Variant, and Smart LifeLC. (bvsalud.org)
Alkaline1
  • Because hemoglobins exhibit different migration patterns depending on the pH level, testing the same sample at both an acid and an alkaline pH can help to identify some abnormal hemoglobins that would otherwise be impossible to distinguish from others. (wikipedia.org)
Clinical1
  • [ 7 ] Small amounts of these variant hemoglobins might not be detected by diagnostic techniques used in most clinical laboratories. (medscape.com)
Detect1
Subunits1
  • Alpha-globin proteins make up two of the four subunits of every hemoglobin. (mastelf.com)
Separation3
  • Applications: for separation of glycoproteins, lipoproteins and hemoglobin from blood. (slideshare.net)
  • Separation of hemoglobins is based on variable rates of migration of charged hemoglobin molecules in an electrical field. (medscape.com)
  • Hemoglobin electrophoresis has been superseded by more rapid, sensitive, and quantitative methods of hemoglobin separation. (medscape.com)
Milder1
Trait1
  • One hundred -thalassaemia carriers, The most reliable methods for diagnosis who were parents of known -thalassaemia of thalassaemia trait include quantitative patients, were enrolled in Group 4 as con- determination of haemoglobin A2 (HbA2), trols. (who.int)
Chains3
  • Unlike adults who have alpha and beta chains in the hemoglobin, a foetus expresses a zeta chain instead of alpha and epsilon, then later gamma instead of the HbA beta chain. (flashcardmachine.com)
  • Hemoglobin is a tetramer with two pairs of globin chains, each containing an identical heme group. (medscape.com)
  • Hemoglobin molecules consist of polypeptide chains whose chemical structure is genetically. (msdmanuals.com)
Diagnosis2
Migration1
  • Different hemoglobins have different charges, and according to those charges, they move at different speeds in the gel and eventually form discrete bands (see electrophoretic migration patterns). (wikipedia.org)
Classification1
Decreases1
  • D-2-3 Bisphosphoglycerate decreases the affinity hemoglobin for oxygen by binding to and stabilizing the deoxy-hemoglobin state. (flashcardmachine.com)
Amounts2
  • The relative amounts of each type of hemoglobin can be estimated by measuring the optical density of the bands, though this method is not reliable for hemoglobins that are present in low quantities. (wikipedia.org)
  • Human skin color varies according to various factors, in particular, season, race, and gender, and is mainly determined by the amounts of melanin, carotene, and hemoglobin, of which melanin acts as the most decisive factor. (justia.com)
Suitable1
Genes1
  • It is caused by homozygous inheritance of genes for hemoglobin (Hb) S. Sickle-shaped red blood cells cause vaso-occlusion and are prone to hemolysis, leading to severe pain crises, organ ischemia, and other systemic complications. (msdmanuals.com)
Distinguish1
Blood4
  • Both the heterozygous and homozygous states may induce erythrocyte dehydration (xerocytosis) and an elevated mean corpuscular hemoglobin concentration (MCHC) may be noted on a complete blood count. (medscape.com)
  • Abstract Introduction: This study aims to determine the association of ABO/ Rh Blood groups and haemoglobin genotype with Helicobacter pylori infection among indigenes of Wiyaakara, Ogoni, Rivers State. (ajlhtsonline.org)
  • This is a cross-sectional study carried out in Wiyaakara, Khana Local Government Area in Rivers State to determine the prevalence of Helicobacter pylori infection and its association with ABO/Rh blood group and haemoglobin genotype. (ajlhtsonline.org)
  • Electrophoresis applied to BLOOD PROTEINS. (bvsalud.org)
Method1
  • The concentration of hemoglobin in the serum is determined by colorimetric method through an assay where the peroxidase activity of hemoglobin is measured using ortho-toluidine as a substrate. (eceratrading.com)
Unit1
Consequences1
Minor1
  • Hemoglobin A2 levels are typically elevated in beta-thalassemia minor and hemoglobin F may be slightly increased. (wikipedia.org)
Affinity2
Oxygen1
Symptoms1
  • Hemoglobin C disease (Hb CC) is a mild disorder that generally does not cause any symptoms and is associated with a normal life expectancy. (medscape.com)
Evaluation1
  • Hemoglobin ß-globin evaluation through gel electrophoresis, high-performance liquid chromatography, and HBB gene sequencing was performed on patients from Texas hospitals between 2010 and 2015 and demographic parameters (age, sex, ethnicity) was subsequently analyzed. (bvsalud.org)
Human1
Production1
  • Thalassemia is a genetic disorder affecting hemoglobin production that could lead to anemia. (mastelf.com)
Results1
  • Equivocal results were those in which there haemoglobin, haematocrit, mean corpuscu- was a very fine cloudiness in the tube and lar volume (MCV) and mean corpuscular the edges of the lines were slightly blurred. (who.int)
Beta2
  • Hemoglobin C (Hb C) is a structural variant of normal hemoglobin A (Hb A) caused by an amino acid substitution of lysine for glutamic acid at position six of the beta hemoglobin chain. (medscape.com)
  • How many alpha and beta sub-units does hemoglobin have? (flashcardmachine.com)
Conditions1
Disease1
  • Cervical vertebrae 1,2, …?Ca: Calcium?Ca: Cancer?Ca: Carcinoma?Ca: Cardiac arrest?Ca: Coronary artery?CA-125: A tumor marker for ovarian cancer?CAB: Cellulose acetate butyrate?CABG: Coronary artery bypass graft?CACI: Computer-Assisted Continuous Infusion?CAD: Coronary artery disease?CAG: ?CAH: Chronic active hepatitis?CAH: Congenital adrenal hyperplasia?calid. (kuwaitpharmacy.com)
Type1
Test1