• donors
  • Similarly, using a lethal irradiation and transplant model, co-infusion of young ECs with whole BM (WBM) cells from young or old donors improved HSC-repopulating activity, engraftment, and survival in recipient mice compared with infusions of donor WBM cells alone. (sciencemag.org)
  • citation needed] In order to harvest stem cells from the circulating peripheral blood, blood donors are injected with a cytokine, such as granulocyte-colony stimulating factor (G-CSF), that induces cells to leave the bone marrow and circulate in the blood vessels. (wikipedia.org)
  • vitro
  • HSC population is then the ideal target for the correction of hematopoietic genetic diseases and also for the knockout of the responsible genes to in vitro and in vivo model those hematopoietic diseases. (intechopen.com)
  • They also showed that isolated c-Kit-positive cells can be expanded in vitro and give rise to new β -cells that secrete insulin in a glucose-responsive fashion [ 11 ]. (hindawi.com)
  • congenital
  • The discovery has implications for developing stem cell-based therapies for diseases like leukemia and congenital blood disorders. (redorbit.com)
  • Candidates for HSCTs include pediatric cases where the patient has an inborn defect such as severe combined immunodeficiency or congenital neutropenia with defective stem cells, and also children or adults with aplastic anemia who have lost their stem cells after birth. (wikipedia.org)
  • vivo
  • They used a specific dose (5uM) of a pharmacologic inhibitor of Cdc42, CASIN, to reduce the protein's activity in the cells - processing them for 16 hours ex vivo in laboratory cultures. (redorbit.com)
  • regeneration
  • G. Nigro, R. Rossi, P. H. Commere, P. Jay, P. J. Sansonetti, The cytosolic bacterial peptidoglycan sensor Nod2 affords stem cell protection and links microbes to gut epithelial regeneration. (sciencemag.org)
  • infusion
  • Newswise - Researchers at University of California San Diego School of Medicine report that a single infusion of wildtype hematopoietic stem and progenitor cells (HSPCs) into a mouse model of Friedreich's ataxia (FA) measurably halted cellular damage caused by the degenerative disease. (newswise.com)
  • When young mice were exposed to myelosuppressive total body irradiation (TBI), infusion of young ECs promoted rapid hematopoietic recovery, whereas infusion of aged ECs promoted an aged phenotype including impaired HSC function and myeloid bias. (sciencemag.org)
  • Adult
  • Recently, it was shown that islet and ductal cells in adult pancreas expressed Sca-1 [ 14 ]. (hindawi.com)
  • In a healthy adult person, approximately 1011-1012 new blood cells are produced daily in order to maintain steady state levels in the peripheral circulation. (wikipedia.org)
  • protein
  • If there is a mutation in the protein, that means that the mitochondria metabolism most likely will be altered and will not be able to provide enough energy to the hematopoietic stem cells. (wikipedia.org)
  • Ubiquitin is a small (76-amino acid) protein highly conserved among eukaryotic cells. (wikipedia.org)
  • epithelial
  • CDCP1 is widely expressed in human epithelial tissues, but its phosphorylation is only seen in mitotically detached or shedding cells, consistent with its role in the negative regulation of cell adhesion. (wikipedia.org)
  • malignant
  • The patient is then treated with high-dose chemotherapy with or without radiotherapy with the intention of eradicating the patient's malignant cell population at the cost of partial or complete bone marrow ablation (destruction of patient's bone marrow's ability to grow new blood cells). (wikipedia.org)
  • lineage
  • Supplementary Figure S1 shows that the majority of detached cells had the morphological characteristics of granulocyte/macrophage lineage cells. (hindawi.com)
  • These complexes are recruited to sites throughout the genome by cell lineage-defining transcription factors (LDTFs) that determine the context-dependent nature of TGF-β action. (wikipedia.org)
  • The lymphoid lineage is primarily composed of T-cells and B-cells (types of white blood cells). (wikipedia.org)
  • tissue
  • These change into macrophages after entering into appropriate tissue spaces, and can transform into foam cells in endothelium. (wikipedia.org)
  • The bedrock of what it means to be a living entity is an understanding of how a cell or tissue functions as an integrated system. (wikipedia.org)
  • regulate
  • It must balance enormous production needs (more than 500 billion blood cells are produced every day) with the need to precisely regulate the number of each blood cell type in the circulation. (wikipedia.org)
  • Many factors produced by other cells can regulate the chemotaxis and other functions of monocytes. (wikipedia.org)
  • blood cells
  • Image Caption: A scanning electron microscope image depicting normal circulating human blood, which includes round, dimpled red blood cells and several types of white blood cells, including lymphocytes, a monocyte and a neutrophil, and many small, disc-shaped platelets. (redorbit.com)
  • They are an essential support mechanism of blood cells and the immune system. (redorbit.com)
  • Also, elevated expression of Cdc42 has been found in immune system white blood cells in older humans. (redorbit.com)
  • As these cells are short-lived, there needs to be a steady turnover of new blood cells and the maintenance of an HSC pool. (wikipedia.org)
  • This model has received support from studies showing subpopulations of blood cells harboring initiating but not late somatic mutations in patients with chronic lymphocytic leukemia (CLL), hairy cell leukemia (HCL), and AML. (wikipedia.org)
  • Hematopoietic stem cells give rise to blood cells. (wikipedia.org)
  • GM-CSF stimulates production of white blood cells. (wikipedia.org)
  • G-CSF is a growth factor for neutrophils (a common type of white blood cells), and may act by increasing the activity of neutrophil-derived proteases such as neutrophil elastase in the bone marrow leading to proteolytic degradation of SDF-1. (wikipedia.org)
  • Erythroid cells are the oxygen carrying red blood cells. (wikipedia.org)
  • In developing embryos, blood formation occurs in aggregates of blood cells in the yolk sac, called blood islands. (wikipedia.org)
  • When bone marrow develops, it eventually assumes the task of forming most of the blood cells for the entire organism. (wikipedia.org)
  • severe
  • Brpf1 -deficient mice display postnatal lethality and severe hematopoietic hypoplasia. (jci.org)
  • Since more than one type of immune cell is affected, this disease is classified as a severe combined immunodeficiency disease (SCID). (wikipedia.org)
  • Infection
  • A. Burberry, M. Y. Zeng, L. Ding, I. Wicks, N. Inohara, S. J. Morrison, G. Núñez, Infection mobilizes hematopoietic stem cells through cooperative NOD-like receptor and Toll-like receptor signaling. (sciencemag.org)
  • Because "most of HIV strains" use the CCR5 receptor to enter a host cell, the mutation confers resistance to HIV infection. (wikipedia.org)
  • human
  • There are at least three types of monocytes in human blood: The classical monocyte is characterized by high level expression of the CD14 cell surface receptor (CD14++ CD16− monocyte) The non-classical monocyte shows low level expression of CD14 and additional co-expression of the CD16 receptor (CD14+CD16++ monocyte). (wikipedia.org)
  • Matthew P. Scott has identified the human homolog PTCH1 as a key tumor suppressor gene for the Hedgehog signaling pathway and the causative gene for Nevoid basal-cell carcinoma syndrome. (wikipedia.org)
  • It is a very efficient inducer of hematopoietic stem cell mobilization in animal and human studies. (wikipedia.org)
  • In a small human clinical trial to evaluate the safety and efficacy of fucoidan ingestion (brown seaweed extract), 3g daily of 75% w/w oral fucoidan for 12 days increased the proportion of CD34+CXCR4+ from 45 to 90% and the serum SDF-1 levels, which could be useful in CD34+ cells homing/mobilization via SDF-1/CXCR4 axis. (wikipedia.org)
  • Zinc finger nucleases have also been used in a mouse model of haemophilia and a clinical trial found CD4+ human T-cells with the CCR5 gene disrupted by zinc finger nucleases to be safe as a potential treatment for HIV/AIDS. (wikipedia.org)
  • The compound immunodeficiencies in NSG branded mice permit the engraftment of a wide range of primary human cells, and enable sophisticated modeling of many areas of human biology and disease. (wikipedia.org)
  • function
  • That NOD signaling influences stem cell function was also reported by Nigro et al . (sciencemag.org)
  • Pharmacologic inhibition of Cdc42 reversed HSC aging and restored function similar to that of younger stem cells, explained Hartmut Geiger, PhD, the study's principal investigator and a researcher in the Division of Experimental Hematology/Cancer Biology at Cincinnati Children's, and the Department of Dermatology and Allergic Diseases, Ulm University Medicine. (redorbit.com)
  • Apart from the proper design of the nucleases to recognize and cut the selected site in the cell genome, two main goals need to be adequately addressed to optimize its function: the delivery of the tools into the desired cells and the selection of those where the gene editing process has occurred correctly. (intechopen.com)
  • The genetic background, derived from inbred NOD mouse strain NOD/ShiLtJ, contributes reductions in innate immunity that include an absent hemolytic complement system, reduced dendritic cell function, and defective macrophage activity. (wikipedia.org)
  • diseases
  • This chapter will review and discuss how researchers are trying to solve them, all attempts that are ongoing and the potential application of the technology to the patients affected with hematopoietic genetic diseases. (intechopen.com)
  • 2016
  • June 2016 BL-8040, a Peptidic CXCR4 Antagonist, Induces Leukemia Cell Death and Specific Leukemia Cell Mobilization in Relapsed/Refractory Acute Myeloid Leukemia Patients in an Ongoing Phase IIa Clinical Trial. (wikipedia.org)
  • tumor
  • Other conditions treated with stem cell transplants include sickle-cell disease, myelodysplastic syndrome, neuroblastoma, lymphoma, Ewing's sarcoma, desmoplastic small round cell tumor, chronic granulomatous disease and Hodgkin's disease. (wikipedia.org)
  • Another explanation of this observation is provided by a study that shows the ability of CXCL12 (and CCL2) producing tumors to entrain neutrophils that inhibit seeding of tumor cells in the lung. (wikipedia.org)
  • Correction
  • The availability of these tools has allowed a precise gene editing, such as knockout of a specific gene or the correction of a defective gene by means of homologous recombination (HR), taking advantage of the endogenous cell repair machinery. (intechopen.com)
  • CXCR4
  • HIV isolates that use CXCR4 are traditionally known as T-cell tropic isolates. (wikipedia.org)
  • It has been also shown that CXCR4 signalling regulates the expression of CD20 on B cells. (wikipedia.org)
  • WHIM like mutations in CXCR4 were recently identified in patients with Waldenstrom's macroglobulinemia, a B-cell malignancy. (wikipedia.org)
  • However, in breast cancer where SDF1/CXCL12 is also expressed by the cancer cells themselves along with CXCR4, CXCL12 expression is positively correlated with disease free (metastasis free) survival. (wikipedia.org)
  • CXCR4 antagonists are especially in important for hindering cancer progression because one of the downstream effects initiated by CXCR4 receptor activation is cell movement which helps the spread of cancer, known as metastasis. (wikipedia.org)
  • survival
  • who found that signaling induced by a microbe-derived agonist of NOD2 promoted survival of intestinal stem cells and improved their resistance to oxidative stress-induced cell death. (sciencemag.org)