• Genetic abnormalities associated with MDS block differentiation of hematopoietic stem and progenitor cells. (medscape.com)
  • Small hepatic progenitor cells (SHPCs) derived from these hepatocytes have shown mature metabolic functions as well as engraftment in immunodeficient mice. (warf.org)
  • 1) Hepatocytes generated in this system display late fetal markers (both albumin and alpha-fetoprotein, AFP) and give rise to mature and engraftable small hepatic progenitor cells (SHPCs, which express albumin but not AFP). (warf.org)
  • Stem and progenitor cell populations are often heterogeneous, which may reflect stem cell subsets that express subtly different properties, including different propensities for lineage selection upon differentiation, yet remain able to interconvert. (lu.se)
  • however, the phenotypic progression of progenitor cells during human embryonic hemogenesis is not well described. (qnrf.org)
  • Here, we define the developmental hierarchy among intermediate populations of hematopoietic progenitor cells (HPCs) derived from human embryonic stem cells (hESCs). (qnrf.org)
  • Progenitor cell therapy describes the use of multipotent cells of various cell lineages (autologous or allogeneic) for tissue repair and/or regeneration. (southcarolinablues.com)
  • Progenitor cell therapy is being investigated for the treatment of damaged myocardium resulting from acute or chronic cardiac ischemia and for refractory angina. (southcarolinablues.com)
  • For individuals who have acute cardiac ischemia who receive progenitor cell therapy, the evidence includes 2 randomized controlled trials (RCTs) with 200 patients, numerous small RCTs, and meta-analyses of these RCTs. (southcarolinablues.com)
  • Overall, this evidence has suggested that progenitor cell treatment may be a promising intervention, but robust data on clinical outcomes are lacking. (southcarolinablues.com)
  • For individuals who have chronic cardiac ischemia who receive progenitor cell therapy, the evidence includes a nonrandomized comparative trial and systematic reviews of smaller RCTs. (southcarolinablues.com)
  • Results of the nonrandomized trial are encouraging, because this is the first controlled trial that has reported a significant mortality benefit for progenitor cell treatment. (southcarolinablues.com)
  • For individuals who have refractory angina who receive progenitor cell therapy, the evidence includes phase 2 trials and a phase 3 pivotal trial. (southcarolinablues.com)
  • Additional larger trials are needed to determine whether progenitor cell therapy improves health outcomes in patients with refractory angina. (southcarolinablues.com)
  • 1 Treatment with progenitor cells (i.e., stem cells) offers potential benefits beyond those of standard medical care, including the potential for repair and/or regeneration of damaged myocardium. (southcarolinablues.com)
  • In fetal liver and adult bone marrow, Tie-2 is expressed by a subpopulation of hematopoietic progenitor cells characterized as Lineage markers^-, c-Kit^+, Sca1^+ cells. (thermofisher.com)
  • Recent studies have isolated distinct cell populations from infantile hemangiomas that display properties equivalent to aberrant progenitor cells, suggesting that, in addition to malignant tumors, benign tumors may also contain a stem cell-like component. (medscape.com)
  • Globally, it is estimated that 120 000 corneal transplantations and 18 000 transplantations of allogeneic haematopoietic progenitor cells took place in the year 2000. (who.int)
  • Likewise, these cells give rise to progenitor cells committed to a particular cell lineage, and play a crucial role in tissue repair and homeostasis. (bvsalud.org)
  • The results showed that the space microgravity environment provided favorable conditions for the 3D growth and maintenance of stemness of mouse embryonic stem cells, and stem cells cultured in space showed a better 3D growth mode than on the ground and maintained a higher level of pluripotent gene expression. (pdnews.cn)
  • Wutz, A. Haploid mouse embryonic stem cells: rapid genetic screening and germline transmission. (nature.com)
  • Forward and reverse genetics through derivation of haploid mouse embryonic stem cells. (nature.com)
  • DNMT3A and TET1 cooperate to regulate promoter epigenetic landscapes in mouse embryonic stem cells. (stembook.org)
  • For pediatric patients with refractory cytopenia, certain cytogenetic abnormalities, or malignant transformation, hematopoietic stem cell transplantation (HSCT) from a matched related or unrelated donor early in the course of the disease is the treatment of choice. (medscape.com)
  • These studies reveal potentially new therapeutic targets for recovery from anemia and regeneration of blood cells for transplantation. (ca.gov)
  • Overview and choice of donor of hematopoietic stem cell transplantation. (mayoclinic.org)
  • How to perform hematopoietic stem cell transplantation. (mayoclinic.org)
  • The goal of this work is to elucidate novel molecular mechanisms that can be therapeutically targeted to regulate cell migration in a tissue-specific fashion - to inhibit it in certain contexts (e.g., inflammatory diseases and cancer metastasis) and promote it in others (e.g., stem cell transplantation and cell-based therapies). (bu.edu)
  • The aims of this article are to review the current knowledge regarding therapeutic mechanisms of mesenchymal stem cells in acute liver failure, to discuss recent advancements in preclinical and clinical studies in the treatment of mesenchymal stem cells, and to summarize the methodological improvement of mesenchymal stem cell transplantation in treating liver failure. (springer.com)
  • Furthermore, transplantation of Lhx2-transduced hematopoietic cells into lethally irradiated mice resulted in multi-lineage repopulation of hematopoietic cells over 4 months. (ebiotrade.com)
  • The induction of a specific differentiated cell type can be useful for transplantation or drug screening and drug discovery in vitro. (justia.com)
  • In particular, the induction of stem cells to differentiate into muscle cells (myocytes) is useful for muscle transplantation and therapeutic purposes, as well as providing potential human disease models in culture (e.g. for testing pharmaceuticals). (justia.com)
  • MDS is rare in childhood and may have a rapidly progressive course with an extremely poor prognosis without hematopoietic stem cell transplantation (HSCT). (medscape.com)
  • 1 This includes human cells for transplantation such as haematopoietic stem cells from bone marrow, peripheral blood or cord blood. (who.int)
  • Gene expression at these histones regulated by epigenetic modifications is critical in restricting the embryonic stem cell to desired cell lineages and developing cellular memory. (wikipedia.org)
  • Aberration occurs in a stem cell that can give rise to multiple lineages. (medscape.com)
  • This event explains the presence of multiple derangements observed in the bone marrow that involve several cell lineages. (medscape.com)
  • MSCs have the capacity of multiple differentiations and self-renewal and have been proven to be able to differentiate into a series of cell lineages, such as adipocytes, osteoblasts, chondroblasts, and hepatocyte-like cells (HLCs). (springer.com)
  • The process of differentiation in stem cells involves selective development of immature cells to committed and fully mature cells of various lineages. (justia.com)
  • At these sites, which are a compound of stromal cells, extracellular matrix and soluble factors, complex molecular interactions that maintain the essential properties of stem cells occur, such as self-renewal and differentiation into multiple lineages, according to the organism's needs. (bvsalud.org)
  • Quality assurance of hematopoietic stem cells by macrophages determines stem cell clonality. (bu.edu)
  • Using a new p16-FDR mouse line, we show that macrophages and endothelial cells are the predominant senescent cell types in murine KRAS-driven lung tumors. (bvsalud.org)
  • Through single cell transcriptomics, we identify a population of tumor-associated macrophages that express a unique array of pro-tumorigenic SASP factors and surface proteins and are also present in normal aged lungs. (bvsalud.org)
  • Macrophages derived from blood monocytes and resident macrophages produced during embryonic development can be activated to amplify control of infection, but they can also produce substances that induce unwanted excessive inflammation. (msdmanuals.com)
  • Usage: Mouse endothelial cells (MS1) and MS1 cells transduced to express full-length human TEM1 (MS1-TEM1) were cultured in 96-well plates to 30% confluence and then incubated for 96h in the presence of 10-fold serially diluted Streptavidin-ZAP, scFv78, or scFv78-ZAP starting from 40nM down to 0.04nM. (atsbio.com)
  • Vascular endothelial junction-associated molecule, a novel member of the immunoglobulin superfamily, is localized to intercellular boundaries of endothelial cells. (taconic.com)
  • EVs were instrumental in transferring selected miRNAs from HLSCs to human endothelial cells absent in the latter cells. (spandidos-publications.com)
  • The horizontal transfer of extracellular RNAs carried by EVs has been shown to be able to reprogram hematopoietic progenitors ( 11 ) and to activate endothelial cells ( 12 ). (spandidos-publications.com)
  • In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. (justia.com)
  • Therefore there remains a need for providing effective methods of inducing differentiation of stem cells into specific cell types, such as myocytes or endothelial cells. (justia.com)
  • The RUNX1 protein turns on (activates) genes that help control the development of blood cells (hematopoiesis). (medlineplus.gov)
  • We portrayed the Notch system in embryonic stem cell (ESC)-derived embryoid bodies (EBs) differentiating under the standard protocols used to assess yolk sac (YS) hematopoiesis in vitro. (karger.com)
  • The inventors have developed a novel system for co-differentiation of hepatocytes and hematopoietic progenitors from human embryonic stem cells (ES cells) that mimics a late fetal liver undergoing hematopoiesis. (warf.org)
  • Dr. Elliott J. Hagedorn is a cell and developmental biologist with expertise in hematopoiesis, stem cell biology and vascular heterogeneity. (bu.edu)
  • Time-series single-cell RNA sequencing paired with single-cell resolution spatial revealed human embryonic development signatures such as extraembryonic placenta, yolk sac hematopoiesis neural crest, blood vessels, and cardiac mesoderm. (newswise.com)
  • A member of the tyrosine kinase receptor family, Tie-2 is expressed on endothelial and a subset of hematopoietic cells and is believed to play a role in both angiogenesis and hematopoiesis during development of the mouse embryo. (thermofisher.com)
  • Myelodysplastic syndrome (MDS) in childhood encompasses a diverse group of bone marrow disorders that share a common clonal defect of stem cells and that result in ineffective hematopoiesis with dysplastic changes in the marrow. (medscape.com)
  • Temporal multimodal single-cell profiling of native hematopoiesis illuminates altered differentiation trajectories with age. (lu.se)
  • IL-5 has been shown to promote the growth of immature hematopoietic BFU-E progenitors, stimulate the activation and differentiation of eosinophils, and promote the generation of cytotoxic lymphocytes. (biolegend.com)
  • mesenchymal stem cells are most commonly used because they are easy to obtain and present no ethical problems. (springer.com)
  • HLSCs do not express hematopoietic stem cell markers, whereas they express several mesenchymal markers and display multiple differentiating abilities ( 22 ). (spandidos-publications.com)
  • While numerous teams continue to refine and expand the role of bone marrow and cord blood stem cells for their vanguard uses in blood and immune disorders, many others are looking to expand the uses of the various types of stem cells found in bone marrow and cord blood, in particular mesenchymal stem cells, to uses beyond those that could be corrected by replacing cells in their own lineage. (biomedcentral.com)
  • There are many studies involving autologous therapies and some allogenic therapies, based on the recovery of mobilized bone marrow cells, including mesenchymal stem cells (MSCs) and adipose derived stem cells that also include the stromal or adherent cell type that has an MSC phenotype. (biomedcentral.com)
  • [ 7 ] Similarly, in 2001, Priolo and Laganà reclassified the ectodermal dysplasias into 2 main functional groups: (1) defects in developmental regulation/epithelial-mesenchymal interaction and (2) defects in cytoskeleton maintenance and cell stability. (medscape.com)
  • The aorta-gonad-mesonephros (AGM) region is of particular interest, as it autonomously generates the first adult type hematopoietic stem cells (HSCs). (ox.ac.uk)
  • examined the effects of epigenetic modifications on the chromatin structure and the modulation of these epigenetic markers during stem cell differentiation through in vitro differentiation of murine embryonic stem (ES) cells. (wikipedia.org)
  • Comparison of toxicity of benzene metabolite hydroquinone in hematopoietic stem cells derived from murine embryonic yolk sac and adult bone marrow. (cdc.gov)
  • In recent years, foreign scientists have also reported several times on research regarding stem cell growth and tissue regeneration carried out during space flight missions, such as blood stem cells for astronauts with anemia. (pdnews.cn)
  • Experts said that the unique space microgravity environment may be a new way to maintain undifferentiated proliferation of stem cells, enhance the efficiency of induced differentiation, and improve the level of three-dimensional tissue construction. (pdnews.cn)
  • Since positional information plays an important role in the embryonic development of several tissue systems, we tested whether AGM HSC induction is influenced by the surrounding dorsal and ventral tissues. (ox.ac.uk)
  • Chimeric explant cultures with genetically distinguishable AGM and ventral tissues show that the increase in HSC activity is not from ventral tissue-derived HSCs, precursors or primordial germ cells (as was previously suggested). (ox.ac.uk)
  • Fig. 1: Design of the MiSTR cell culture system and establishment of a WNT signaling gradient in hESC-derived tissue. (nature.com)
  • This means they can potentially produce new cells for any organ or tissue. (healthline.com)
  • Background: Stem cells are of two types: embryonic and adult stem cells and they act as a repair system by replenishing body tissue. (benthamscience.com)
  • Discussion: Activation of the PI3K/AKT pathway decreases the concentration of apoptotic factors, while the HIF pathway protects stem cells against the micro-environment of tissue (hypoxia). (benthamscience.com)
  • Ramalingam, M. Surface functionalization of nanobiomaterials for application in stem cell culture, tissue engineering, and regenerative medicine. (benthamscience.com)
  • These early trials are showing roles for stem cells both in replacing damaged tissue as well as in providing extracellular factors that can promote endogenous cellular salvage and replenishment. (biomedcentral.com)
  • The use of patient's own bone marrow aspirates, hematopoietic stem cells and MSCs, for heart muscle tissue repair can be puzzling because these cells do not normally contribute to the cardiac lineage types that are desired. (biomedcentral.com)
  • Embryonic stem (ES) cells are derived from the embryo and are pluripotent, thus possessing the capability of developing into any organ, cell type or tissue type. (justia.com)
  • Methods of inducing differentiation in stem cells and muscle cells produced therefrom may be used for the study of cellular and molecular biology of tissue development, for the discovery of genes and proteins such as differentiation factors that play a role in tissue development and regeneration. (justia.com)
  • Glioma-associated oncogene 1 expressing (Gli1+) cells are a major source of activated fibroblasts in multiple organs, but the links between injury, inflammation, and Gli1+ cell expansion and tissue fibrosis remain incompletely understood. (bvsalud.org)
  • The use of gametes, embryonic and fetal tissue as well as blood and blood products raises additional questions that need to be separately addressed. (who.int)
  • Niches are special microenvironments in tissue where stem cells are located. (bvsalud.org)
  • Likewise, the knowledge of stem cell biology is crucial to the development of stem cell therapies, based on tissue engineering applied to dentistry, seeking the regeneration of dental tissues damaged or lost by caries, trauma or genetic diseases. (bvsalud.org)
  • This stem cell will further mature and differentiate to form a group of hematopoietic stem cells resembling a cluster of grapes. (pdnews.cn)
  • This regulation of chromatin through epigenetic modifications is a molecular mechanism that determines whether the cell continues to differentiate into the desired fate. (wikipedia.org)
  • Embryonic stem cells containing reduced or elevation levels of methylation are viable but unable to differentiate and therefore require critical regulation of cytosine methylation for mammalian development. (wikipedia.org)
  • Eventually, the cells begin to differentiate, taking on a certain function in a part of the body. (healthline.com)
  • Adult stem cells can't differentiate into as many other types of cells as embryonic stem cells can. (healthline.com)
  • They can differentiate into all types of specialized cells in the body. (healthline.com)
  • The breakthrough has created a way to "de-differentiate" the stem cells. (healthline.com)
  • The nature of the stem cell substates and their relationship to commitment to differentiate and lineage selection can be elucidated in terms of a landscape picture in which stable states can be defined mathematically as attractors. (lu.se)
  • Stem cells differentiate into different types of cells, such as neural, hematopoietic, adipose, etc. and are used for the treatment of various conditions like myocardial infarction, spinal cord injury, Parkinson's disease and diabetes. (benthamscience.com)
  • This includes bone and cartilage repair, cell types into which MSCs readily differentiate, and immune conditions such as graft versus host disease and autoimmune conditions that utilize the MSC's immune suppressive properties. (biomedcentral.com)
  • Generally, when a stem cell culture is induced to differentiate, the differentiated population is analysed for particular cell types by expression of genes, markers or phenotypic analysis. (justia.com)
  • Dental pulp stem cells have been isolated from deciduous and permanent teeth and have the potential to self-renew and differentiate. (bvsalud.org)
  • Ventral embryonic tissues and Hedgehog proteins induce early AGM hematopoietic stem cell development. (ox.ac.uk)
  • Our explant culture results at early and late embryonic day 10 show that ventral tissues induce and increase AGM HSC activity, whereas dorsal tissues decrease it. (ox.ac.uk)
  • Fig. 3: Single-cell transcriptomics of dorsal and ventral MiSTR tissues. (nature.com)
  • Cells Tissues Organs (2011) 193 (4): 239-252. (karger.com)
  • These stem cells come from developed organs and tissues in the body. (healthline.com)
  • Research in the Hagedorn Lab utilizes a combination of genetics, genomics and advanced live cell imaging in zebrafish to understand how blood and cancer cells migrate into and out of distinct tissues and organs. (bu.edu)
  • In this study, we compared hematopoietic differentiation capacity of 35 human iPSC lines derived from four different tissues and four embryonic stem cell lines. (nih.gov)
  • The term "oncotarget" encompasses all molecules, pathways, cellular functions, cell types, and even tissues that can be viewed as targets relevant to cancer as well as other diseases. (oncotarget.com)
  • We established the human embryonic organoid (HEMO) from expanded potential stem cells and achieved both embryonic and extraembryonic tissues in the same organoid. (newswise.com)
  • Hematopoietic tissues eventually predominated HEMO with erythropoiesis, mekagaryopiesis, and myelopoiesis. (newswise.com)
  • Cell-cell communication network analysis demonstrated that trophoblast-like tissues supplied WNT signaling in neural crest cells to facilitate maturation and migration. (newswise.com)
  • Understanding how stem cells behave in the niche is extremely important in order to extract these cells from their natural habitat, expand them in vitro and transplant the stem cells back to the patient, to repair and/or regenerate tissues and organs, with no risks to the individual's integrity. (bvsalud.org)
  • Therapies based on the application of stem cells have great potential in the prevention and treatment of several diseases, such as cancer, diabetes, cardiovascular disease, spinal cord injuries, neurological diseases such as Parkinson's and Alzheimer's, and in the regeneration of various tissues and organs. (bvsalud.org)
  • The ectodermal dysplasias (EDs) comprise a large, heterogeneous group of inherited disorders that are defined by primary defects in the development of 2 or more tissues derived from embryonic ectoderm. (medscape.com)
  • The differentiated hematopoietic precursors express mature markers that show promise for engraftment (study in progress). (warf.org)
  • The analysis revealed that hematopoietic commitment capacity (PSCs to hematopoietic precursors) is correlated with the expression level of the IGF2 gene independent of the iPSC origins. (nih.gov)
  • During differentiation the expression of stem cell specific genes and markers are often lost and cells acquire gene expression profiles of somatic cells or their precursors. (justia.com)
  • The ventral position of hematopoietic clusters closely associated with the aorta of most vertebrate embryos suggests a polarity in the specification of AGM HSCs. (ox.ac.uk)
  • Leeb, M. & Wutz, A. Derivation of haploid embryonic stem cells from mouse embryos. (nature.com)
  • Embryonic stem cells come from human embryos that are three to five days old. (healthline.com)
  • Spatiotemporal analysis of stem-cell-derived embryos offers conceptual and technical advances in the field. (newswise.com)
  • This change in gene activity blocks the maturation (differentiation) of blood cells and leads to the production of abnormal, immature white blood cells called myeloid blasts. (medlineplus.gov)
  • Stem cell homeostasis is maintained through epigenetic mechanisms that are highly dynamic in regulating the chromatin structure as well as specific gene transcription programs. (wikipedia.org)
  • Although safety remains a concern, investigators are breaking ground in cell and gene therapy, and many believe that ultimately, a string of cancer cures will follow. (ajmc.com)
  • Although only a handful of cell and gene therapies are on the market, FDA officials predicted in 2019 that the agency will receive more than 200 investigational new drug applications per year for cell and gene therapies, and that by 2025, it expects to have accelerated to 10 to 20 cell and gene therapy approvals per year. (ajmc.com)
  • Gene therapy modifies or manipulates cell expression. (ajmc.com)
  • Juliette Hordeaux, PhD, senior director of translational research for the University of Pennsylvania's gene therapy program, is cautious about the FDA's predictions, saying she'd be "thrilled" with 5 cell and/or gene therapy approvals annually. (ajmc.com)
  • Safety has been the main brake around adeno-associated virus vector (AAV) gene therapy," added Hordeaux, whose hospital's program has the institutional memory of both Jesse Gelsinger's tragic death during a 1999 gene therapy trial as well as breakthroughs by Carl June, MD, and others in CAR T-cell therapy. (ajmc.com)
  • Induced pluripotent stem (iPS) cells have great potential for regenerative medicine and gene therapy. (stemcell.com)
  • Putative "stemness" gene jam-B is not required for maintenance of stem cell state in embryonic, neural, or hematopoietic stem cells. (taconic.com)
  • Here, we defined the single-cell spatiotemporal gene expression landscape of human embryonic development with stem-cell-derived organoids. (newswise.com)
  • We are studying the role that normal chromatin structure plays in gene regulation in hematopoietic cells and how its disruption leads to altered development and cancer. (stanford.edu)
  • This can provide more beneficial assistance for the future use of stem cell regeneration to serve human health. (pdnews.cn)
  • From the experimental results, China has successfully achieved the first hematopoietic differentiation of human embryonic stem cells under space conditions, accomplishing its first experimental goal. (pdnews.cn)
  • Here, we model early human neural tube development using human embryonic stem cells cultured in a microfluidic device. (nature.com)
  • Derivation and differentiation of haploid human embryonic stem cells. (nature.com)
  • A novel characterisation and separation technique for pluripotent human embryonic and hematopoietic stem cells. (ukri.org)
  • Erythropoiesis from human embryonic stem cells through erythropoietin-independent AKT signaling. (ca.gov)
  • Human PSC can produce red cells in culture but the process is currently inefficient producing too few cells for practical use. (ca.gov)
  • The goal of the present study was to investigate the potential of ic-MPL dimerization to induce erythropoiesis from human embryonic stem cells (hESC) and to identify the signaling pathways activated by this strategy. (ca.gov)
  • However, in recent years, there has been controversy surrounding the way human embryonic stem cells are obtained. (healthline.com)
  • Recombinant Human Wnt-3a (Catalog # 5036-WN) induces alkaline phosphatase production by the MC3T3-E1 mouse preosteoblast cell line. (novusbio.com)
  • Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. (novusbio.com)
  • Because APE1/Ref-1 is expressed and elevated in prostate cancer, we sought to characterize APE1/Ref-1 expression and activity in human prostate cancer cell lines and determine the effect of selective reduction-oxidation (redox) function inhibition on prostate cancer cells in vitro and in vivo . (oncotarget.com)
  • Our study demonstrates that survivin and APE1/Ref-1 are significantly higher in human prostate cancer specimens compared to noncancerous controls and that APE1/Ref-1 redox-specific inhibition with small molecule inhibitor, APX3330 and a second-generation inhibitor, APX2009, decreases prostate cancer cell proliferation and induces cell cycle arrest. (oncotarget.com)
  • Single-cell level characterization of embryonic development is a major benchmark of human developmental biology. (newswise.com)
  • Overall, our study advances the spatiotemporal analysis of human embryonic development in stem-cell-derived organoids. (newswise.com)
  • The aim of the present study was to evaluate whether Alix is involved in the packaging of miRNAs within EVs released by human liver stem‑like cells (HLSCs). (spandidos-publications.com)
  • Vesicles derived from adult human liver stem-like cells (HLSCs) have also been shown to contain ribonucleoproteins and different RNA species involved in hepatic regeneration ( 20 ). (spandidos-publications.com)
  • By developing novel tools and harnessing the power of metabolomics, proteomics and functional genomics, our lab will define 1) how the lysosome communicates with other cellular compartments to fulfill the metabolic demands of the cell under various metabolic states, 2) and how its dysfunction leads to rare and common human diseases. (stanford.edu)
  • Many existing protocols for neuronal differentiation of human pluripotent cells result in heterogeneous cell populations and unsynchronized differentiation, necessitating the development of methods for labeling specific cell populations. (lu.se)
  • We provide detailed protocols for lentiviral transduction, neural differentiation, and subsequent analysis of human embryonic stem cells. (lu.se)
  • Although possible, these strategies are often complicated to transfer to human cells due to technical issues (9 ), and only a few successful cases have been described ( 10, 11 ). (lu.se)
  • Here we describe how this system can be used for human embry- onic stem cells. (lu.se)
  • Human CMV grows only in human cells and replicates best in human fibroblasts. (medscape.com)
  • In particular, it plays an important role in development of hematopoietic stem cells, early blood cells that have the potential to develop into all types of mature blood cells such as white blood cells, red blood cells, and platelets. (medlineplus.gov)
  • While t(8;21) is important for leukemia development, a mutation in one or more additional genes is typically needed for the myeloid blasts to develop into cancerous leukemia cells. (medlineplus.gov)
  • The mammalian epigenome undergoes global remodeling during early stem cell development that requires commitment of cells to be restricted to the desired lineage. (wikipedia.org)
  • Notch receptors and Notch ligands were detected in virtually all cells throughout EB development. (karger.com)
  • Methods: This article focuses on recent research development that addresses the viability issues of stem cells. (benthamscience.com)
  • His work is focused on T cell receptor signaling and the development of T cell subsets. (rupress.org)
  • However, the molecular pathways that lead to specification and terminal differentiation of specific cell types, such as myocytes, from embryonic stem cells during development are not entirely clear. (justia.com)
  • The ligand angiopoietin-1 binds to this receptor and mediates a signaling pathway that functions in embryonic vascular development. (thermofisher.com)
  • However, further studies are required to gain complete understanding of stem cell biology, which is fundamental for the development of successful cell-based therapies 1-3 . (bvsalud.org)
  • In 2003, Lamartine reclassified the ectodermal dysplasias into the following 4 functional groups based on the underlying pathophysiologic defect: (1) cell-to-cell communication and signaling, (2) adhesion, (3) development, and (4) other. (medscape.com)
  • [ 8 ] Other classification systems categorize the ectodermal dysplasias based on defects in cell-cell communication and signaling, adhesion, transcription regulation, or development. (medscape.com)
  • Histone modifications of H3K9 position show a decrease in di- and tri-methylation of undifferentiated embryonic stem cells and had a gradual increase in methylation during the six-day time course of in vitro differentiation, which indicated that there is a global increase of inactive heterochromatin levels at this histone mark. (wikipedia.org)
  • In addition, the red cells produced are of an immature type, expressing mostly embryonic rather than adult hemoglobin. (ca.gov)
  • Current methods to induce erythropoiesis from PSC suffer from low yields of RBCs, most of which are immature and contain embryonic and fetal rather than adult hemoglobins. (ca.gov)
  • AKT activation upregulates the GATA-1 and FOXO3 transcriptional pathways with resulting inhibition of apoptosis, modulation of cell cycle and enhanced maturation of erythroid cells. (ca.gov)
  • Understanding cell-fate decisions in stem cell populations is a major goal of modern biology. (lu.se)
  • American Journal of Respiratory Cell and Molecular Biology. (lu.se)
  • At last, scientists had devised a way to reprogram a person's own T cells to attack tumor cells. (ajmc.com)
  • The key to curing every single cancer, which is our goal, is to have receptors that can recognize the tumor but don't recognize the normal cells. (ajmc.com)
  • The accumulation of senescent cells in the tumor microenvironment can drive tumorigenesis in a paracrine manner through the senescence-associated secretory phenotype (SASP). (bvsalud.org)
  • Genetic or senolytic ablation of senescent cells, or macrophage depletion, result in a significant decrease in tumor burden and increased survival in KRAS-driven lung cancer models. (bvsalud.org)
  • Our research focuses on developmental pathways that regulate hematopoietic cell growth and differentiation and are disrupted in the course of neoplastic transformation, particularly in leukemias and lymphomas. (stanford.edu)
  • Placing the cells under conditions which induce specific cell types has been one form of an attempt to regulate the differentiation outcome. (justia.com)
  • For mammalian cells, the maintenance of cytosine methylation is catalyzed by DNA methyltransferases and any disruption to these methyltransferases will cause a lethal phenotype to the embryo. (wikipedia.org)
  • Nine projects were funded totalling £5 million and mainly focused on mammalian cell culture. (ukri.org)
  • Experiments revealed that the fusion protein activates unusual signaling pathways during PSC differentiation leading to changes in cycling and survival of the red cells. (ca.gov)
  • Conclusion: For the protection of stem cells against apoptosis, different pathways, namely Phosphoinositide 3-Kinase (PI3K/AKT), Hypoxia-Inducible Factor (HIF1), Mitogen-Activated Protein Kinases (MAPK) and Hippo, are discussed. (benthamscience.com)
  • There has been a rapid surge in clinical trials involving stem cell therapies over the last two to three years and those trials are establishing the clinical pathways for an emergent new medicine. (biomedcentral.com)
  • Extracellular vesicles (EVs), including exosomes, are membranous particles released by cells into the extracellular space. (mdpi.com)
  • The efficiency of transplanted stem cells reduces due to conditions like hypoxia, inflammation, nutrient deprivation, immunogenicity, extracellular matrix loss on delivery and mechanical stress. (benthamscience.com)
  • culturing a stem cell in the presence of an embryonic cell and/or extracellular medium of an embryonic cell, under conditions that induce differentiation of the stem cell. (justia.com)
  • There has been multiple evidence suggesting that the maintenance of the lineage commitment of stem cells is controlled by epigenetic mechanisms such as DNA methylation, histone modifications and regulation of ATP-dependent remolding of chromatin structure. (wikipedia.org)
  • These results indicate a decrease in the level of active euchromatin epigenetic marks upon initiation of embryonic stem cell differentiation which is then followed immediately by reprogramming of the epigenome. (wikipedia.org)
  • The EV-mediated transfer of miRNAs is a particularly good candidate for the epigenetic alterations observed in recipient cells. (spandidos-publications.com)
  • hPSC-enriched essential genes mainly encode transcription factors and proteins related to cell-cycle and DNA-repair, revealing that a quarter of the nuclear factors are essential for normal growth. (nature.com)
  • Fig. 2: Analysis of cell-essential genes. (nature.com)
  • This mechanism also applies to hematopoietic cells transformed by other HOX genes, including CDX2, which is highly expressed in a majority of acute myeloid leukemias, thus providing a molecular approach based on GSK-3 inhibitory strategies to target HOX-associated transcription in a broad spectrum of leukemias. (stanford.edu)
  • Cell-specific transcriptional control of mitochondrial metabolism by TIF1? (bu.edu)
  • Due to the role of oncogenic transcriptional activators NFĸB and STAT3 in survivin protein expression, and APE1/Ref-1 redox activity regulating their transcriptional activity, we assessed selective inhibition of APE1/Ref-1's redox function as a novel method to halt prostate cancer cell growth and survival. (oncotarget.com)
  • We demonstrate here that GSK-3 maintains the MLL leukemia stem cell transcriptional program by promoting the conditional association of CREB and its coactivators TORC and CBP with homedomain protein MEIS1, a critical component of the MLL-subordinate program, which in turn facilitates HOX-mediated transcription and transformation. (stanford.edu)
  • Single-cell transcriptomics revealed that rostro-caudal organization was already established at 24 h of differentiation, and that the first markers of a neural-specific transcription program emerged in the rostral cells at 48 h. (nature.com)
  • Fig. 4: Single-cell transcriptomics of early MiSTR patterning and temporal dissection of MiSTR regionalization. (nature.com)
  • Single-cell resolution spatial transcriptomics defined the yolk sac erythro-megakaryopoietic niche. (newswise.com)
  • Single-cell transcriptomics shows that during the endothelial-to-hematopoietic transition, Ncx1 −/− cells fail to. (lu.se)
  • Single-cell transcriptomics shows that during the endothelial-to-hematopoietic transition, Ncx1 −/− cells fail to undergo a glycolysis to oxidative phosphorylation metabolic switch present in wild-type cells. (lu.se)
  • In addition to these basic issues concerning leukemia pathogenesis, we are devising new diagnostic procedures for detecting and monitoring leukemia patients based on molecular genetic abnormalities in the malignant cells. (stanford.edu)
  • The "stem cell theory of cancer" states that a subpopulation of cells with stem cell-like properties plays a central role in the formation, sustainment, spread, and drug resistant characteristics of malignant tumors. (medscape.com)
  • These findings provide supporting evidence that enrichment for proteins involved in pluripotency is not restricted solely to malignant tumors as is suggested by the "stem cell theory of cancer", but additionally extends to common benign vascular tumors such as hemangiomas. (medscape.com)
  • Genetic modification and screening in rat using haploid embryonic stem cells. (nature.com)
  • These cells have been successfully used to treat children with blood cancers, such as leukemia, and certain genetic blood disorders. (healthline.com)
  • Therefore, EVs may carry genetic information that may be shared among cells ( 11 - 14 ). (spandidos-publications.com)
  • Resolving the Pathogenesis of Anaplastic Wilms Tumors through Spatial Mapping of Cancer Cell Evolution. (lu.se)
  • Here we describe how microRNA-regulated lentiviral vectors can be used to visualize specific cell populations by exploiting endogenous microRNA expression patterns. (lu.se)
  • To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)
  • They may also serve as an unlimited source of therapeutically active cells for regenerative medi- cine. (lu.se)
  • citation needed] As the embryonic stem cell undergoes differentiation the markers for active euchromatin (histone acetylation and H3K4 methylation) are decreased after the removal of LIF showing that the cell is indeed becoming more differentiated. (wikipedia.org)
  • The slight rebound in each of these marks allows for further differentiation to occur by allowing another opportunity to decrease the markers once again, bringing the cell closer to its mature state. (wikipedia.org)
  • IL-5 is a homodimeric, disulphide-linked protein produced by T-cells. (biolegend.com)
  • In this paper we found that if we introduced a synthetic "fusion" protein into PSC, we could produce significantly more red cells in culture and that those cells were also more mature. (ca.gov)
  • We also found that the synthetic protein acts to increase red cell production independently of the well known growth factor erythropoietin. (ca.gov)
  • Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. (novusbio.com)
  • Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels (2). (novusbio.com)
  • Aifantis, I. Regulation of stem cell function by protein ubiquitylation. (benthamscience.com)
  • These data demonstrated that variations in the hematopoietic differentiation capacity of iPSCs are not attributable to somatic memories of their origins. (nih.gov)
  • Transduction of Lhx2 into ESC-derived mesodermal cells resulted in robust differentiation of c-Kit+/Sca-1+/Lineage- (KSL) cells in vitro. (ebiotrade.com)
  • They establish a link between clonal expansion and lineage plasticity of T cells and reveal the involvement of granzyme K-expressing CD8 T cells in TPE pathogenesis. (rupress.org)