• T4 replication helicase (gp41) interacts with the polymerase holoenzyme at the fork, stabilizing the other protein components and being stabilized as a result. (neb.com)
  • It forms a complex with T7 DNA polymerase. (wikipedia.org)
  • Learn the basic functions of the following DNA replication enzymes: helicase, primase, ligase, polymerase I and III. (ti.com)
  • Then polymerase alpha starts with the synthesis of primers, which consequently become elongated by polymerase delta and epsilon for regular DNA synthesis. (specklab.com)
  • DNA Polymerase III Mainenzymeinvolvedinreplication DIMER. (slideshare.net)
  • Deze primers zijn essentieel voor de synthese van DNA, aangezien DNA-polymerase alleen nucleotiden kan toevoegen aan een bestaande streng. (jove.com)
  • DNA-polymerase draagt bij aan de groeiende dochterstrengen op beide template-DNA-strengen. (jove.com)
  • Verwijder na de synthese van de nieuwe strengen, RNase H of aanvullende varianten van DNA-polymerase, de primers en synthetiseer DNA in hun plaats. (jove.com)
  • We identify an important role for DDX5 in G 1 -S-phase progression where it directly regulates DNA replication factor expression by promoting the recruitment of RNA polymerase II to E2F-regulated gene promoters. (aacrjournals.org)
  • CpGV encodes six genes required for genome replication, which includes DNA polymerase and helicase. (kenyon.edu)
  • Tunability of DNA polymerase stability during eukaryotic DNA replication. (rockefeller.edu)
  • New strands are made using the enzyme polymerase, and ligase is the enzyme that connects the short segments of nucleotides in a newly made DNA strand. (slideshare.net)
  • Hamdan SM, Marintcheva B, Cook T, Lee SJ, Tabor S, and Richardson CC. A unique loop in T7 DNA polymerase mediates the binding of helicase-primase, DNA binding protein, and processivity factor. (edu.sa)
  • To test this idea, we have measured the extent of processivity of the 41 protein in the context of an in vitro DNA replication system composed of eight purified proteins (the gene 43, 44/62, 45, 32, 41, 59, and 61 proteins). (neb.com)
  • After starting DNA replication in the presence of these proteins, we diluted the 41 helicase enough to prevent any association of new helicase molecules and analyzed the replication products. (neb.com)
  • 1. Unwindingof DNADuplex:  Helicase enzyme  Topoisomerase  Single Stranded Binding Proteins 1. (slideshare.net)
  • E proteins made by the viral genome promote the activation of host DNA replication mechanisms that can then be used by the virus during its own replication. (medscape.com)
  • DDX5 is required for cell proliferation by controlling the transcription of genes expressing DNA replication proteins in cancer cells in which the DDX5 locus is amplified, and this has uncovered a dependence on DDX5 for cell proliferation. (aacrjournals.org)
  • DNA replication origins retain mobile licensing proteins. (yeastgenome.org)
  • We find that this type of non-canonical fork convergence in fission yeast is prone to trigger deletions between repetitive DNA sequences via a mechanism we call Inter-Fork Strand Annealing (IFSA) that depends on the recombination proteins Rad52, Exo1 and Mus81, and is countered by the FANCM-related DNA helicase Fml1. (elifesciences.org)
  • and the contribution of chromatin-modifying proteins to replication and repair. (usc.edu)
  • We have reconstituted chromatin replication with purified proteins, which is providing unprecedented insights into chromosome biology. (crick.ac.uk)
  • We have reconstituted the process of chromatin replication with purified proteins. (crick.ac.uk)
  • The duplication of chromosomes and the regulation of their replication and repair requires dozens of proteins acting together to maintain the duplex DNA genome. (rockefeller.edu)
  • Proteins in the family to which SMBP2 belongs (known as the DNA helicase/ATPase family), are known to be involved in many cellular activities, including DNA replication, repair, and recombination. (sciencedaily.com)
  • It then recruits several other proteins in a stepwise fashion that, together, unzip and prime the DNA for replication. (sbstatesman.com)
  • Somewhat surprisingly, it turns out this mechanism is related to a previous mechanism we studied for loading other DNA replication proteins onto DNA. (sbstatesman.com)
  • The larger Rep proteins, Rep78 and Rep68, assemble into stable, hexameric oligomers on specific secondary structures on the 3' and 5' termini of the single stranded viral DNA. (utoledo.edu)
  • The smaller replication proteins, Rep40 and Rep52, require ATP to bind DNA, show no sequence specificity in DNA binding and lack the N-terminal nuclease domains. (utoledo.edu)
  • Our studies are aimed at understanding how these proteins assemble on DNA structures and the specificity of their interaction with DNA. (utoledo.edu)
  • This protein is a major component of the primosome, being essential for coordinated leading and lagging strand DNA synthesis. (neb.com)
  • We measured an association half-life of 11 min, revealing that the 41 protein is processive enough to finish replicating the entire 169-kilobase T4 genome at the observed replication rate of approximately 400 nucleotides/s. (neb.com)
  • This processivity of the 41 protein does not require the 59 protein, the protein that catalyzes 41 protein assembly onto 32 protein-covered single-stranded DNA. (neb.com)
  • The stability we measure for the 41 protein as part of the replication fork is greater than estimated for it alone on single-stranded DNA. (neb.com)
  • T7 DNA helicase (gp4) is a hexameric motor protein encoded by T7 phages that uses energy from dTTP hydrolysis to process unidirectionally along single stranded DNA, separating (helicase) the two strands as it progresses. (wikipedia.org)
  • Future work will define structural changes that occur during replication fork assembly the define the protein bindings sites on the MCM2-7 double-hexamer. (specklab.com)
  • The BLM gene provides instructions for making a member of a protein family called RecQ helicases. (medlineplus.gov)
  • The BLM protein helps to prevent excess sister chromatid exchanges and is also involved in other processes that help maintain the stability of the DNA during the copying process. (medlineplus.gov)
  • Without the BLM protein, the cell is less able to repair DNA damage caused by ultraviolet light, which results in increased sun sensitivity. (medlineplus.gov)
  • The ORC-Cdc6 complex (product 1) assembles in step 1 around origin DNA and with the help of another replication initiator protein, Cdt1, it recruits the Mcm2-7 hexamer to the origin in step 2. (nature.com)
  • It is caused by pathogenic variants in the WRN gene, which encodes a multifunctional nuclear protein with exonuclease and helicase activities. (amrita.edu)
  • WRN protein is thought to be involved in optimization of various aspects of DNA metabolism, including DNA repair, recombination, replication, and transcription. (amrita.edu)
  • Changing protein-DNA interactions promote ORC binding-site exchange during replication origin licensing. (yeastgenome.org)
  • Rif1 controls DNA replication by directing Protein Phosphatase 1 to reverse Cdc7-mediated phosphorylation of the MCM complex. (yeastgenome.org)
  • Structural changes in Mcm5 protein bypass Cdc7-Dbf4 function and reduce replication origin efficiency in Saccharomyces cerevisiae. (yeastgenome.org)
  • We are using this to understand the mechanism of MCM helicase loading and activation, how the replisome is assembled and regulated by protein kinases and how the DNA replication machinery interfaces with other aspects of chromosome biology. (crick.ac.uk)
  • O'Donnell's laboratory studies these DNA metabolic mechanisms with the goal of understanding how the protein gears of DNA duplication act together with signal processes that function with repair and recombination factors to ensure genomic integrity. (rockefeller.edu)
  • She completed her PhD with her project entitled "The Roles of the Helicase Double-Hexamer Complex and the ssDNA-Binding Protein RPA During Eukaryotic DNA Replication" in Steve Bell's lab at MIT. (mit.edu)
  • The Neuron paper reports that the defective gene (designated Smbp2) in the neuromuscular disease mice encodes a DNA-binding protein (SMBP2, or immunoglobulin S-mu binding protein-2) on chromosome 19. (sciencedaily.com)
  • The group's latest work is part of series of studies published in the journal Genes and Development last month that has begun to uncover the protein machinery responsible for DNA replication. (sbstatesman.com)
  • A short time after, Steve Bell, then a postdoc in my lab, identified a protein that binds to the origin of replication, which was named ORC. (sbstatesman.com)
  • This initiator protein ORC, or origin replication complex, first binds to the DNA. (sbstatesman.com)
  • In their latest study the group looked at the structure of the helicase, a ring-like protein responsible for unwinding the double-stranded DNA into two single strands. (sbstatesman.com)
  • E1 protein has helicase activity for replication, and E2 encodes DNA-binding protein for regulation of transcription. (medscape.com)
  • They form into a protein complex that has helicase activity and is involved in a variety of DNA-related functions including replication elongation, RNA transcription, chromatin remodeling, and genome stability. (bvsalud.org)
  • The protein is likely to be involved in the response to DNA damage during replication, as well as in the replication and transcription processes. (medscape.com)
  • A reconstituted system reveals how activating and inhibitory interactions control DDK dependent assembly of the eukaryotic replicative helicase. (specklab.com)
  • It has been unclear at the molecular level how Cdc6 activates ORC, converting it to an active recruiter of the Mcm2-7 hexamer, the core of the replicative helicase. (nature.com)
  • Validation of a high throughput screening assay to identify small molecules that target the eukaryotic replicative helicase. (yeastgenome.org)
  • Bell SD and Botchan MR (2013) The minichromosome maintenance replicative helicase. (yeastgenome.org)
  • The Dbf4-dependent Cdc7 kinase (DDK) initiates replisome assembly by phosphorylating the MCM2-7 replicative helicase at the N-terminal tails of Mcm2, Mcm4 and Mcm6. (imperial.ac.uk)
  • The six subunit Origin Recognition Complex (ORC) together with Cdc6 and Cdt1, load the MCM replicative helicase as a double hexamer around double stranded DNA at origins. (crick.ac.uk)
  • [ 10 ] Fragile sites may be DNA regions particularly sensitive to replicative stress. (medscape.com)
  • It is also a primase, making short stretches of RNA that initiates DNA synthesis. (wikipedia.org)
  • Promising new inhibitors that target the viral helicase-primase complex have been reported to block replication of herpes simplex and varicella-zoster viruses, but they have no activity against human cytomegalovirus (HCMV), another herpesvirus. (frontiersin.org)
  • The HCMV helicase-primase complex (pUL105-pUL102-pUL70) is essential for viral DNA replication and could thus be a relevant antiviral target. (frontiersin.org)
  • In HCMV, the herpesvirus helicase-primase complex is composed of a helicase (pUL105), a primase (pUL70) and a primase-associated factor (pUL102) ( McMahon and Anders, 2002 ). (frontiersin.org)
  • Lee J-B, Hite RK, Hamdan SM, Xie XS, Richardson CC, and van Oijen AM. DNA primase acts as a molecular brake in DNA replication. (edu.sa)
  • and single-stranded DNA binding activity. (nih.gov)
  • Predicted to contribute to single-stranded DNA helicase activity. (nih.gov)
  • During origin firing, the MCM helicase is activated, which involves a remodeling of the MCM ring to encircle single-stranded DNA, accompanied by assembly of a stable CMG (Cdc45-MCM-GINS) complex. (crick.ac.uk)
  • Replication fork activation is enabled by a single-stranded DNA gate in CMG helicase. (rockefeller.edu)
  • It has a small (4500 nucleotides) genome that encoding four DNA helicases that are required for viral DNA replication and efficient packaging of single stranded DNA into virions. (utoledo.edu)
  • Rep52 and Rep40 are implicated in packaging plus and minus single stranded DNA into virions. (utoledo.edu)
  • When a cell prepares to divide to form two cells, the DNA that makes up the chromosomes is copied so that each new cell will have two copies of each chromosome, one from each parent. (medlineplus.gov)
  • Trinucleotide repeats are a source of genome instability, causing replication fork stalling, chromosome fragility, and impaired repair. (tufts.edu)
  • Eukaryotic DNA replication initiates at multiple origin sites along each chromosome and terminates when replication forks (RFs) from adjacent origins converge. (elifesciences.org)
  • http://dx.doi.org/10.1083/jcb.201410061 ) report that DNA replication initiates at measurable frequency within the telomere of mouse chromosome arm 14q. (rupress.org)
  • However, at the end of the chromosome, the gap after removal of the 5′ terminal RNA primer on the lagging strand cannot be filled in, and the chromosome may become shorter with each ensuing round of replication. (rupress.org)
  • Evidence was recently presented of a crucial role for a helicase in protecting cells against chromosome breakage at normally occurring replication fork-stalling sites. (medscape.com)
  • In addition, in a helicase-deficient mutant, Rad51-dependent JMs are detected, probably corresponding to recombination between sisters. (tufts.edu)
  • Recombination and Pol ζ Rescue Defective DNA Replication upon Impaired CMG Helicase-Pol ε Interaction. (yeastgenome.org)
  • Collapsed forks can be rescued by homologous recombination, which restarts replication. (elifesciences.org)
  • This so-called recombination-dependent replication (RDR) helps ensure that DNA is fully replicated prior to sister chromatid segregation, thereby avoiding mitotic catastrophes. (elifesciences.org)
  • Studies the molecular mechanisms of DNA replication, recombination, and repair. (rockefeller.edu)
  • Applying this multidisciplinary approach will provide a true molecular understanding of the mechanisms involved in replication, repair, and recombination. (edu.sa)
  • Exchange of DNA between chromosomes derived from the individual's mother and father are also increased in people with BLM gene mutations. (medlineplus.gov)
  • Telomeres and DNA Replication Telomeres: Theendsof eukaryoticchromosomes (chromosomes arelinear) Neededfor chromosomal integrity andstability. (slideshare.net)
  • The Origin Recognition Complex (ORC) binds to sites in chromosomes to specify the location of origins of DNA replication. (nature.com)
  • In the budding yeast Saccharomyces cerevisiae , there are over 400 origins of DNA replication located on 16 chromosomes and they can function as autonomously replicating sequences (ARSs) when inserted into a plasmid 19 . (nature.com)
  • We aim to understand how the 46 chromosomes in our cells are precisely duplicated in each cell cycle, how this process responds to DNA damage and how it is misregulated in cancer. (crick.ac.uk)
  • Eukaryotic chromosomes are duplicated via semiconservative replication with a leading (continuous synthesis for net growth at the 3′ end of the nascent leading strand) and lagging (discontinuous Okazaki fragment synthesis for net growth at the 5′ end of the nascent lagging strand) elongating strand as shown in Fig. 1 . (rupress.org)
  • Indeed, a number of agents currently used in cancer treatment are known to target DNA synthesis. (aacrjournals.org)
  • Regardless of its precise form, collapse renders the fork incompetent for further DNA synthesis. (elifesciences.org)
  • They demonstrate that resolution of G4 structures on the G-rich template strand of the telomere requires some overlapping functions of BLM and WRN helicase for leading strand synthesis. (rupress.org)
  • Hamdan SM, Loparo JJ, Takahashi M, Richardson CC, and van Oijen AM. Dynamics of DNA replication loops reveal temporal control of lagging-strand synthesis. (edu.sa)
  • Reference: Processivity of the gene 41 DNA helicase at the bacteriophage T4 DNA replication fork. (neb.com)
  • Models suggest that such DNA helicases are loaded only onto DNA at origins of replication, and that they remain with the ensuing replication fork until replication is terminated. (neb.com)
  • The MCM2-7 double-hexamer is the final product of pre-RC formation and represents the assembly platform for the replication fork. (specklab.com)
  • The MCM2-7 double-hexamer serves as the assembly platform for the entire replication fork. (specklab.com)
  • We conclude that Srs2 interaction with PCNA allows the helicase activity to unwind fork-blocking CAG/CTG hairpin structures to prevent breaks. (tufts.edu)
  • These results clarify the many roles of Srs2 in facilitating replication through fork-blocking hairpin lesions. (tufts.edu)
  • Replication fork barriers are a commonly encountered problem, which can cause fork collapse and act as hotspots for replication termination. (elifesciences.org)
  • However, replication restart is relatively slow and, therefore, replication termination may frequently occur by an active fork converging on a collapsed fork. (elifesciences.org)
  • RDR can initiate from a collapsed fork where the DNA is either broken or remains intact. (elifesciences.org)
  • Recently, the group identified the centromere as a natural fragile site in the genome, particularly when the replication fork is disrupted. (usc.edu)
  • The DNA strands are positioned right in front of the two Mcm2-Mcm5 gates, with each strand being pressed against one gate. (specklab.com)
  • TYPES OF REPLICATION 6 Hypothetically, therecouldbethreepossible waysthat DNAreplication occur: Conservative replication: Bothparental strands stay together afterDNAreplication. (slideshare.net)
  • DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. (jove.com)
  • Because RecQ helicases help maintain the structure and integrity of DNA, they are known as the "caretakers of the genome. (medlineplus.gov)
  • Rothmund-Thomson syndrome (poikiloderma congenitale) has been attributed to mutations of the RECQL4 gene on 8q24, which encodes a RecQ DNA helicase. (medscape.com)
  • [ 3 ] which codes for a member of the RecQ family of DNA helicases. (medscape.com)
  • WS have been classified is a member of the RecQ family of DNA helicases implicated in the resolution of DNA structures leading to the stall of replication forks. (medscape.com)
  • In this lesson, students will use simulations to interact with DNA replication in order to explore semi-conservative replication and identify specific enzymes and their roles in replication. (ti.com)
  • Enzymes in this group unwind double helix RNA and DNA. (medscape.com)
  • Specialized helicases play an important role in unwinding DNA structures to maintain genome stability. (tufts.edu)
  • We applied an assay that measures the stability of maintenance of an episomal plasmid in human tissue culture cells to screen for new DNA replication factors. (aacrjournals.org)
  • Miller CLW and Winston F (2023) The conserved histone chaperone Spt6 is strongly required for DNA replication and genome stability. (yeastgenome.org)
  • Forsburg takes a holistic approach to studying how DNA replication stress contributes to genome stability. (usc.edu)
  • The controlled assembly of replication forks is critical for genome stability. (imperial.ac.uk)
  • We examine the impact that processes such as transcription and replication have on genome stability. (birmingham.ac.uk)
  • His lab studies how the replisome interacts with DNA repair and cell cycle checkpoint machinery, how initiation of replication is directed by nucleosomes, and how replication forks perform nucleosome inheritance, the process by which nucleosomes (the fundamental structural unit of chromosomal DNA) are passed down from parental to daughter DNA during replication. (rockefeller.edu)
  • Previously it was thought that DNA replication began at an origin in chromosomal DNA adjacent to the telomere repeats, with the replication forks moving bidirectionally away from the subtelomeric origin ( Fig. 1 A ), thus replicating the telomere. (rupress.org)
  • Completion of genome duplication is challenged by structural and topological barriers that impede progression of replication forks. (ku.dk)
  • 1. Genes are made of molecules of DNA which is made of nucleotides that have a phosphate group, a sugar, and a nitrogenous base. (slideshare.net)
  • The Srs2 helicase unwinds DNA hairpins, facilitates replication, and prevents repeat instability and fragility. (tufts.edu)
  • 5. Helicase is the enzyme that unwinds DNA during replication. (slideshare.net)
  • Exhibits a Magnesium-dependent ATP-dependent DNA-helicase activity that unwinds single- and double-stranded DNA in a 3'-5' direction. (lu.se)
  • A cartoon of a section of chromatin in the nucleus with replication origins in three different states. (crick.ac.uk)
  • Together, these data indicate that restoration of DNA or chromatin integrity at loci prone to replication problems requires mitotic transmission to the next cell generations. (ku.dk)
  • At present, it remains poorly understood how DDK docks onto the helicase and how the kinase targets distal Mcm subunits for phosphorylation. (imperial.ac.uk)
  • While each DNA blueprint, or gene, encodes for a different machine or structure, the genome, the collection of DNA wrapped up inside the nucleus, is much more active than a quiet library of neatly stacked blue-prints. (birmingham.ac.uk)
  • This gene belongs to the forkhead family of transcription factors which is characterized by a distinct DNA-binding forkhead domain. (cancerindex.org)
  • Rothmund-Thomson syndrome, or poikiloderma congenitale, is a rare autosomal recessive disorder attributed to mutations of the RECQL4 helicase gene on 8q24. (medscape.com)
  • Rep78, Rep68, Rep52 and Rep40 share common structural elements in their helicase domains, but differ at their N-termini and C-termini as a result of differential splicing and different mRNAs arising from the use of different transcription start sites. (utoledo.edu)
  • The Forsburg lab uses a mixture of classical genetics, molecular biology, and state-of-the-art microscopy to investigate how defects in replication contribute to genome instability during normal cell growth and during the differentiation process of meiosis. (usc.edu)
  • Familia de proteínas que en un principio fueron identificadas en el SACCHAROMYCES CEREVISIAE como elementos esenciales para el mantenimiento de la estructura de microcromosomas00. (bvsalud.org)
  • From structure to mechanism - understanding initiation of DNA replication. (specklab.com)
  • DDK regulates replication initiation by controlling the multiplicity of Cdc45-GINS binding to Mcm2-7. (yeastgenome.org)
  • Bryant JA and Aves SJ (2011) Initiation of DNA replication: functional and evolutionary aspects. (yeastgenome.org)
  • Our research is defining the normal mechanisms of DNA replication and repair, with the aim of understanding how it is deregulated in cancer cells. (birmingham.ac.uk)
  • All HPV subtypes contain early (E) genes and late (L) genes, which are essential to viral integration and replication. (medscape.com)
  • E genes are responsible for episomal replication. (medscape.com)
  • The way the DNA is structured, wrapped and read is fundamental to how and when the blueprint genes are read. (birmingham.ac.uk)
  • The S. cerevisiae ORC binds to specific DNA sequences throughout the cell cycle but becomes active only when it binds to the replication initiator Cdc6. (nature.com)
  • The CMG helicase is composed of Cdc45, Mcm2-7 and GINS. (rcsb.org)
  • This demonstrated that the two Mcm2/5 DNA gates within the MCM2-7 double-hexamer are not aligned along the axis, but offset, suggesting a novel mechanism for helicase activation. (specklab.com)
  • Here we determined the structure of the MCM2-7 double-hexamer bound to DNA using cryo-electron microscopy at 3.9Å resolution. (specklab.com)
  • The work of this international collaboration involving the Li, Stillman and Speck labs, revealed how the MCM2-7 complex specifically interacts with DNA. (specklab.com)
  • In summary, our work provides fundamental insights into DDK structure, control and selective activation of the MCM2-7 helicase during DNA replication. (imperial.ac.uk)
  • Stillman helped pioneer one of the first cell-free DNA replication systems, for which he was awarded the 2010 Louisa Gross Horwitz Prize for Biology or Biochemistry. (sbstatesman.com)
  • When the copying is imperfect, mutations can arise in the DNA of a cell. (sbstatesman.com)
  • While the cancerous effects of changes in DNA have long been known, understanding the way mutations (and normal DNA) are replicated would require scientists to use a purified cell-free system. (sbstatesman.com)
  • Here, using SRS2 separation-of-function alleles, we show that in the absence of Srs2 recruitment to PCNA or in helicase-deficient mutants, breakage at a CAG/CTG repeat increases. (tufts.edu)
  • In chromosomal semiconservative replication, the short 5′ RNA primer is removed from the nascent strand and the gap is filled in by DNA that is ligated to the adjacent nascent DNA. (rupress.org)
  • Semiconservative replication occurs before the action of telomerase. (rupress.org)
  • Promoters, enhancers and physical interactions between regions of DNA are important in ways that are only now becoming apparent, directing phases of development, and often perturbed in cancers. (birmingham.ac.uk)
  • It has DNA helicase and ATPase activity. (nih.gov)
  • Here, we show that mild replication stress increases the frequency of chromosomal lesions that are transmitted to daughter cells. (ku.dk)
  • Avarello, Mario Davide Maria (2021): Characterization and investigation of DNA replication helicase defect as a consequence of aneuploidy. (uni-muenchen.de)
  • Dukaj L and Rhind N (2021) The capacity of origins to load MCM establishes replication timing patterns. (yeastgenome.org)
  • We use a variety of human cell models to understand how the deregulation of normal replication control mechanisms leads to genomic instability and whether this plays a role in cancer biology. (crick.ac.uk)
  • We are studying how genome integrity is preserved and the consequences of modifying the program of DNA replication. (umu.se)
  • Although this can seriously undermine genome integrity, the fate of DNA with unresolved replication intermediates is not known. (ku.dk)
  • Dr. Shen's research has been focused on the roles for cell growth signaling pathways (including RAS-MAPK and PI3K-mTOR) in the regulation of cell cycle progression and DNA damage repair. (cityofhope.org)
  • HPV is now known to be a small deoxyribonucleic acid (DNA) virus that infects epithelial cells and causes a variety of skin lesions. (medscape.com)
  • Differential requirement of Srs2 helicase and Rad51 displacement activities in replication of hairpin-forming CAG/CTG repeats. (tufts.edu)
  • Research from O'Donnell's lab has provided an overview of how the replication machine, or replisome, functions in Escherichia coli , yeast, and humans. (rockefeller.edu)
  • The team employs strategies such as super resolution microscopy to observe individual replisomes in real time during DNA replication in living cells, and cryo-electron microscopy of DNA-replisome and other replisome-associated complexes. (rockefeller.edu)
  • Replisome speed determines the efficiency of the Tus−Ter replication termination barrier. (edu.sa)
  • A helicase-tethered ORC flip enables bidirectional helicase loading. (yeastgenome.org)
  • disorders of the DNA replication machinery. (nih.gov)
  • Scientists have started to get an idea about how helicase is loaded and how it functions in DNA replication machinery. (sbstatesman.com)
  • The replication machinery must open up the barrel, load the helicase onto DNA and then activate it. (sbstatesman.com)
  • With a better understanding of the helicase structure, scientists have started to get an idea about how the helicase is loaded and how it functions once part of the replication machinery. (sbstatesman.com)
  • The DNA within the nuclei of our cells carries the information to generate the machinery of the cell, the cell itself, our tissues and then the whole human. (birmingham.ac.uk)
  • Results showed BRCA1 changes shape in order to protect vulnerable DNA until the copying machinery can be restarted. (birmingham.ac.uk)
  • Here we report the cryo-EM structure at 3.3 Å resolution of the yeast ORC-Cdc6 bound to an 85-bp ARS1 origin DNA. (nature.com)
  • The structure reveals that Cdc6 contributes to origin DNA recognition via its winged helix domain (WHD) and its initiator-specific motif. (nature.com)
  • Yeast heterochromatin regulators Sir2 and Sir3 act directly at euchromatic DNA replication origins. (yeastgenome.org)
  • It largely started with York Marahrens, a Stony Brook graduate student in the late 1980s, who published the first detailed analysis of the origin of replication in the budding yeast," Stillman said. (sbstatesman.com)
  • Upon addition of MCM10, the CMG complex becomes activated and DNA unwinding can occur. (specklab.com)
  • Recommendations for Childhood Cancer Screening and Surveillance in DNA Repair Disorders. (nih.gov)
  • DNA repair. (tufts.edu)
  • These studies can be expected to provide new insights into eukaryotic replication, repair, and epigenetic inheritance. (rockefeller.edu)
  • The proper copying and repair of DNA to maintain the correct sequence and structure of genomes is critical to the proper function of cells, and repair and replication is frequently disrupted in cancers and targeted by cancer treatments. (birmingham.ac.uk)
  • In addition, in some patients with a personal or family history of breast and ovarian cancer, the protective role of BRCA1 in DNA-copying is disabled - while its break repair function is still active. (birmingham.ac.uk)
  • Inhibiting BRAF Oncogene-Mediated Radioresistance Effectively Radiosensitizes BRAFV600E-Mutant Thyroid Cancer Cells by Constraining DNA Double-Strand Break Repair. (cityofhope.org)
  • Participates in DNA replication and may participate in repair. (lu.se)
  • De openingen tussen de fragmenten worden vervolgens afgedicht door DNA-ligase om een continue streng te genereren. (jove.com)
  • Problems that arise during DNA replication can drive genomic alterations that are instrumental in the development of cancers and many human genetic disorders. (elifesciences.org)
  • We show that the number of such 53BP1 nuclear bodies increases after genetic ablation of BLM, a DNA helicase associated with dissolution of entangled DNA. (ku.dk)
  • He Z, Houghton PJ, Williams TM, Shen C* . Regulation of DNA duplication by the mTOR signaling pathway. (cityofhope.org)
  • Mutational analysis of several of these amino acids both in pUL105 and pUL70, proved that they are crucial for viral replication. (frontiersin.org)
  • Current HBV therapeutics, such as nucleoside analogs, effectively suppress viral replication but are not curative. (cdc.gov)