• TRPV1
  • Control experiments with non TRPV1 channel expressing HEK cells as well as experiments with the TRPV1 channel blocker ruthenium red validated the specificity of the observed impedance decrease. (springer.com)
  • In summary, we developed a quantitative impedimetric monitoring system for the analysis of TRPV1 channel activity as well as downstream-induced biological activity in living cells. (springer.com)
  • αβ meATP (50μM) did not evoke a Ca 2+ response in HEK293 cells transfected with TRPV1, however, the TRPV1 agonist, capsaicin (1 μM ), evoked a robust Ca 2+ influx. (umich.edu)
  • agonist
  • Examples of such experiments include: Effects of a drug on sodium channels Inducible RNA interference system Isoform-selective protein kinase C agonist Interaction between two proteins Nuclear export signal in a protein A more specific use of HEK 293 cells is in the propagation of adenoviral vectors. (wikipedia.org)
  • gene
  • The SLC7A11 gene has been found not only in the brain, but has also been found to be expressed in the spinal cord, pancreas, and in glioma cells. (wikipedia.org)
  • glial
  • The dimer cystine gets taken up by glial cells and the monomer of cystine, cysteine, is taken up by neurons. (wikipedia.org)
  • Because of serine and glycine's role as neurotrophic factors in the developing brain, PHGDH has been shown to have high expression in glial and astrocyte cells during neural development. (wikipedia.org)
  • genes
  • Viruses offer an efficient means of delivering genes into cells, which they evolved to do, and are thus of great use as experimental tools. (wikipedia.org)
  • This danger can be avoided by the use of viruses which lack key genes, and which are thus unable to replicate after entering a cell. (wikipedia.org)
  • In order to propagate such viral vectors, a cell line that expresses the missing genes is required. (wikipedia.org)
  • Since HEK 293 cells express a number of adenoviral genes, they can be used to propagate adenoviral vectors in which these genes (typically, E1 and E3) are deleted, such as AdEasy. (wikipedia.org)
  • protein
  • As an initial approach to address whether TWEAK might act as a tumor angiogenesis factor, we established several human embryonic kidney cell lines that constitutively secrete a soluble TWEAK protein and examined their growth properties in vitro and in vivo . (aacrjournals.org)
  • vitro
  • We found that although TWEAK-overexpressing cells do not have a growth advantage in vitro , they form larger and more highly vascularized tumors in athymic mice when compared with control, vector-transfected cells. (aacrjournals.org)
  • As a consequence, HEK 293 cells should not be used as an in vitro model of typical kidney cells. (wikipedia.org)
  • kidney
  • Human embryonic kidney cells 293, also often referred to as HEK 293, HEK-293, 293 cells, or less precisely as HEK cells, are a specific cell line originally derived from human embryonic kidney cells grown in tissue culture. (wikipedia.org)
  • HEK 293 cells were generated in 1973 by transformation of cultures of normal human embryonic kidney cells with sheared adenovirus 5 DNA in Alex van der Eb's laboratory in Leiden, the Netherlands. (wikipedia.org)
  • Graham and coworkers provided evidence that HEK 293 cells and other human cell lines generated by adenovirus transformation of human embryonic kidney cells have many properties of immature neurons, suggesting that the adenovirus preferentially transformed a neuronal lineage cell in the original kidney culture. (wikipedia.org)
  • A comprehensive study of the genomes and transcriptomes of HEK 293 and five derivative cell lines compared the HEK 293 transcriptome with that of human kidney, adrenal, pituitary and central nervous tissue. (wikipedia.org)
  • Given the location of the adrenal gland (adrenal means "next to the kidney"), a few adrenal cells could plausibly have appeared in an embryonic kidney derived culture, and could be preferentially transformed by adenovirus. (wikipedia.org)
  • Adenoviruses transform neuronal lineage cells much more efficiently than typical human kidney epithelial cells. (wikipedia.org)
  • therapeutic
  • Since they are involved in a broad range of crucial cell functions, they represent important therapeutic targets. (springer.com)
  • however
  • However, the original adenovirus transformation was inefficient, suggesting that the cell that finally produced the HEK 293 line may have been unusual in some fashion. (wikipedia.org)
  • several
  • Graham performed the transformation a total of eight times, obtaining just one clone of cells that were cultured for several months. (wikipedia.org)
  • channels
  • Voltage clamp methods have been used to show how action potentials in DRG cells are shaped by TTX-resistant sodium channels. (wikipedia.org)
  • shown
  • In humans cells, SIRT7 has only been shown to interact with two other molecules: RNA polymerase I (RNA Pol I) and upstream binding factor (UBF). (wikipedia.org)
  • production
  • Given that PHGDH represents the committed step in the production of serine in the cell, flux through the pathway must be carefully controlled. (wikipedia.org)