Some other important members of Solanaceae include a number of ornamental plants such as Petunia, Browallia, and Lycianthes, and sources of psychoactive alkaloids, Datura, Mandragora (mandrake), and Atropa belladonna (deadly nightshade). (wikipedia.org)
Atropa belladonna is a medicinal plant and main commercial source of tropane alkaloids (TAs) including scopolamine and hyoscyamine, which are anticholine drugs widely used clinically. (bvsalud.org)
Scopolamine1
Tropane alkaloids (TA) including anisodamine, anisodine, hyoscyamine and scopolamine are a group of important anticholinergic drugs with rapidly increasing market demand, so it is significant to improve TA production by biotechnological approaches. (biomedcentral.com)
Biosynthesis4
Background: Since alkaloids are nitrogenous compounds, the availability of nitrogen [N] is expected to play an important role in the biosynthesis and accumulation of alkaloids in plants. (bvsalud.org)
This review aims to provide practical references for synthetic biologists and highlight the great commercial potential of plant chassis biosynthesis, such as hairy roots. (bvsalud.org)
We propose that ERF1A, CYP90G1-1 and CYP76AH1 are specifically involved in the key steps of steroidal alkaloid biosynthesis in V. maackii roots. (bvsalud.org)
Our data represent the first cross-species analysis of steroidal alkaloid biosynthesis in the genus Veratrum and indicate that the metabolic properties of V. maackii and V. nigrum are broadly conserved despite their distinct alkaloid profiles. (bvsalud.org)
Genes4
In the present study, the co-expression of AaPMT and AaTRI genes in A. acutangulus hairy roots significantly improved the yields of TA and showed higher antioxidant activity than control because of higher total TA content, which is the first report on simultaneous introduction of PMT and TRI genes into TA-producing plant by biotechnological approaches. (biomedcentral.com)
Hence, these two genes are promising target sites for genetic engineering to increase TA in A. acutangulus hairy root cultures. (biomedcentral.com)
Digital expression patterns results suggested that the 4 genes including ODC, ADC, AIH and CPA involved in the upstream pathway of TAs, and the 2 branch pathway genes including SPDS and TRII were found to be expressed in all the detected tissues with high expression level in secondary root. (bvsalud.org)
Most candidate genes were expressed at higher levels in roots than leaves but showed a consistent profile across both species. (bvsalud.org)
Production3
It has been proven that the application of small scale jar fermenters for culturing hairy roots induced from several Solanaceous plants is a very prospective method for production of TA [ 9 - 12 ]. (biomedcentral.com)
The development of the plant hairy root tissue culture system has accelerated the commercialization and industrialization of synthetic biology in the production of plant-derived natural products. (bvsalud.org)
Liliales) is a genus of perennial herbs known for the production of unique bioactive steroidal alkaloids. (bvsalud.org)
Higher1
As compared to the control and single gene transformed lines (P or T lines), PT transgenic hairy root lines produced significantly higher levels of TA. (biomedcentral.com)
Widely1
Tropane alkaloids (TAs) are widely distributed in the Solanaceae, while some important medicinal tropane alkaloids (mTAs), such as hyoscyamine and scopolamine, are restricted to certain species/tribes in this family. (bvsalud.org)
Species1
We also provide evidence that branching genes leading to mTAs likely have evolved in early ancestral Solanaceae species but have been lost in most of the lineages, with A. belladonna and D. stramonium being exceptions. (bvsalud.org)