• vitro
  • To create physiologically relevant in vitro models that support normal cell growth and function, the components of the in vivo environment must be incorporated. (corning.com)
  • HDLs inhibit expression of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin both in vitro 4-6 and in vivo 3,7,8 in a process that may be secondary to a suppression of NF-κB activity. (ahajournals.org)
  • The bone marrow is a rich source of mesenchymal cells, which can be differentiated in vitro into Schwann cells and subsequently engrafted into the damaged nerve. (hindawi.com)
  • In vitro expansion of hematopoietic stem cells. (miltenyibiotec.com)
  • In vitro differentiation of CD4 + T cells towards T H 17 cells and proliferation of T H 17 cells. (miltenyibiotec.com)
  • Fetal bovine serum is the most widely used serum-supplement for the in vitro cell culture of eukaryotic cells. (wikipedia.org)
  • Fetal bovine serum is commercially available from many manufacturers, and because cells grown in vitro are highly sensitive, customers usually test specific batches to check for suitability for their specific cell type. (wikipedia.org)
  • Using human pluripotent stem cells to create in vitro cerebral organoids allows researchers to summarize current developmental mechanisms for human neural tissue as well as study the roots of human neurological diseases. (wikipedia.org)
  • Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself. (wikipedia.org)
  • Parts of an organ or a whole organ can be cultured in vitro. (wikipedia.org)
  • And after the plantlet develops in vitro, then the ex-planting process can often be very stressful and result in plant death, which isn't a problem for pre-acclimated plantlets grown via photoautotrophic tissue culture (In this context, pre-acclimated refers to plantlets that have developed a cuticle and have been cultivated in an open air system as opposed to the closed system seen in typical operations). (wikipedia.org)
  • They showed that opposing gradients of bone morphogenetic protein (BMP) and Nodal, two transforming growth factor family members that act as morphogens, are sufficient to induce molecular and cellular mechanisms required to organize, in vivo or in vitro, uncommitted cells of the zebrafish blastula animal pole into a well-developed embryo. (wikipedia.org)
  • They form characteristic cell clusters in suspension culture that express a set of genes associated with pluripotency and can differentiate into endodermal, ectodermal and mesodermal cells both in vitro and in vivo. (wikipedia.org)
  • In Vitro Cellular & Developmental Biology - Animal is a journal of the Society for In Vitro Biology and publishes articles reporting results of research in cellular, molecular, and developmental biology that employ or are relevant to organs, tissue, tumors, and cells in vitro. (wikipedia.org)
  • The Society for In Vitro Biology was founded in 1946 as the Tissue Culture Association to foster the exchange of knowledge of in vitro biology of cells, tissues, and organs from both plants and animals (including humans). (wikipedia.org)
  • Somatic embryos are mainly produced in vitro and for laboratory purposes, using either solid or liquid nutrient media which contain plant growth regulators (PGR's). (wikipedia.org)
  • induces
  • On the basis of these findings, we speculated that exposure of endothelial cells to rHDLs induces expression of a protein with the potential to protect against a subsequent inflammatory insult. (ahajournals.org)
  • We now show that incubation of human coronary artery endothelial cells (HCAECs) with either rHDLs or native HDLs induces expression of the antioxidant protein, 3β-hydroxysteroid-Δ24 reductase (also known as 24-dehydrocholesterol reductase - DHCR24) in an SR-BI dependent manner. (ahajournals.org)
  • Radiation induces hair loss through damage to hair follicle stem cell progenitors and alteration of keratin expression. (wikipedia.org)
  • endothelial cells
  • Objective- The purpose of this study was to investigate the ability of high-density lipoproteins (HDLs) to upregulate genes with the potential to protect against inflammation in endothelial cells. (ahajournals.org)
  • 4,5,9 We now provide evidence that an HDL-mediated inhibition of VCAM-1 in activated endothelial cells is via blockade of signaling through the classical NF-κB pathway. (ahajournals.org)
  • 6,10 Furthermore, the ability of rHDLs to inhibit VCAM-1 expression in TNFα-activated human umbilical vein endothelial cells (HUVECs) persists even if the rHDLs are removed from the culture medium several hours before the cells are activated by TNFα. (ahajournals.org)
  • However, it is unknown whether a combination of high glucose and thrombin can modulate the expression of NAPDH oxidase (Nox) subtypes in human aortic endothelial cells (HAECs). (hindawi.com)
  • explants
  • Indirect embryogenesis occurs when explants produced undifferentiated, or partially differentiated, cells (often referred to as callus) which then is maintained or differentiated into plant tissues such as leaf, stem, or roots. (wikipedia.org)
  • 2017
  • In January 2017, scientists from Salk Institute for Biological Studies managed to create a pig embryo that had part of its DNA, critical for the growth of organs, edited out. (wikipedia.org)
  • progenitor
  • They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent-iMSC, also called an induced multipotent progenitor cell-iMPC) or unipotent-(iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)
  • neural
  • Below is an image showing some of the chemical factors that can lead stem cells to differentiate into various neural tissues. (wikipedia.org)
  • Neural glial cells cover a wide variety of neural cells, some of which move around the neurons. (wikipedia.org)
  • Cell fate potential Cross species developmental timing Cerebral organoids provide a unique insight into the timing of development of neural tissues and can be used as a tool to study the differences across species. (wikipedia.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • factors
  • The high growth in the Asian market is attributed to factors such as favorable regulatory guidelines, lower manufacturing costs, growing presence of market players in emerging economies, and favorable government investments in the life sciences sector. (pharmiweb.com)
  • This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. (wikipedia.org)
  • Serum is stored frozen to preserve the stability of components such as growth factors. (wikipedia.org)
  • Stem cells have the potential to grow into many different types of tissues and their fate is dependent on many factors. (wikipedia.org)
  • Furthermore, they do not always induce cellular transformation, and are not the only growth factors that induce cellular transformation. (wikipedia.org)
  • Likewise, because hollow fiber bioreactors use up significantly less medium and growth factors than traditional cell culture methods such as stirred-tank bioreactors, they offer a significant cost savings. (wikipedia.org)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)
  • somatic
  • Induced stem cells (iSC) are stem cells derived from somatic, reproductive, pluripotent or other cell types by deliberate epigenetic reprogramming. (wikipedia.org)
  • Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • Somatic embryogenesis is an artificial process in which a plant or embryo is derived from a single somatic cell or group of somatic cells. (wikipedia.org)
  • Somatic embryos are formed from plant cells that are not normally involved in the development of embryos, i.e. ordinary plant tissue. (wikipedia.org)
  • Shoots and roots are monopolar while somatic embryos are bipolar, allowing them to form a whole plant without culturing on multiple media types. (wikipedia.org)
  • medium
  • We recommend using DMEM plus 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium. (thermofisher.com)
  • Can Recovery Cell Culture Freezing Medium undergo multiple freeze thaw cycles? (thermofisher.com)
  • Can Recovery Cell Culture Freezing Medium be stored at 2 to 8°C? (thermofisher.com)
  • Recovery Cell Culture Freezing Medium should be stable at 2 to 8°C for 1 week, although we have no data to support this. (thermofisher.com)
  • Is it important to centrifuge and aspirate Recovery Cell Culture Freezing Medium before seeding into the flask? (thermofisher.com)
  • Recovery Cell Culture Freezing Medium is meant for general cell culture applications where a wide range of growth media will be used. (thermofisher.com)
  • We recommend that you aspirate Recovery Cell Culture Freezing Medium before seeding, although other protocols can be substituted (this will need to be determined by the end user). (thermofisher.com)
  • Prior to staining with trypan blue Solution, do the cells need to be washed to remove the culture medium? (thermofisher.com)
  • If the background is too dark, cells should be pelleted and resuspended in protein-free medium, buffer, or normal saline prior to counting. (thermofisher.com)
  • These cells have a plating efficiency of approximately 22% in the recommended culture medium. (atcc.org)
  • Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes. (atcc.org)
  • Remove and discard culture medium. (atcc.org)
  • Sometimes many cells are floating, they can be harvested by centrifugation of medium instead of discarding it. (atcc.org)
  • Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting. (atcc.org)
  • Cells were cultured in spinner flasks in serum-free medium (60 mL) containing either insulin at 10 mg/L, LONG ® R 3 IGF-I at 50 μg/L or no growth factor for a period of 11 days in a modified fed-batch process. (sigmaaldrich.com)
  • To quantify the number of cells in a culture, the cells can be simply plated on a petri dish with growth medium. (wikipedia.org)
  • The techniques for organ culture can be classified into (i) those employing a solid medium and (ii) those employing liquid medium. (wikipedia.org)
  • The medium with agar provides the mechanical support for organ culture. (wikipedia.org)
  • Cell lines with mutated DHFR are useful for genetic manipulation as cells transfected with a gene of interest along with a functional copy of the dhfr gene can easily be screened for in thymidine-lacking medium. (wikipedia.org)
  • The plasmid DNA carrying the two genes is then transfected into cells, and the cells are grown under selective conditions in thymidine-lacking medium. (wikipedia.org)
  • One of the cell populations is fed with growth medium containing normal amino acids. (wikipedia.org)
  • In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
  • Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
  • Cell culture medium is pumped through the IC space and delivers oxygen and nutrients to the cells via hollow fiber membrane perfusion. (wikipedia.org)
  • As the cells expand, their waste products and CO2 also perfuse the hollow fiber membranes and are carried away by the pumping of medium through the IC space. (wikipedia.org)
  • As waste products build up due to increased cell mass, the rate of medium flow can also be increased so that cell growth is not inhibited by waste product toxicity. (wikipedia.org)
  • Similar to a mammalian lung, the gas exchange cartridge efficiently oxygenated the culture medium, allowing the bioreactor to support higher numbers of cells. (wikipedia.org)
  • Plant growth regulators in the tissue culture medium can be manipulated to induce callus formation and subsequently changed to induce embryos to form from the callus. (wikipedia.org)
  • The auxin typically used is 2,4-D. Once transferred to a medium with low or no auxin, these cells can then develop into mature embryos. (wikipedia.org)
  • apoptosis
  • Analogous to the effect of erythropoietin (EPO), the primary mode of action of TPO is inhibition of apoptosis of its target cells. (miltenyibiotec.com)
  • Miltenyi Biotec
  • Miltenyi Biotec technologies are used in applications concerned with accessing, analyzing, and utilizing primary and primary-derived cells - across basic research, translational research, and clinical applications. (wikipedia.org)
  • With a market share of 70%, Miltenyi Biotec is the leader in the field of magnetic cell separation. (wikipedia.org)
  • Miltenyi Biotec has expanded its portfolio to include a novel microchip-based cell sorting technology with the acquisition of Santa Barbara-based Owl biuomedical, Inc. in April 2013. (wikipedia.org)
  • grown
  • In one case, a cerebral organoid grown from a patient with microcephaly demonstrated related symptoms and revealed that apparently the cause is overly rapid development, followed by slower brain growth. (wikipedia.org)
  • this makes them the preferred counting method in fast biological experiments in which it needs to be merely determined whether a cell culture has grown as expected. (wikipedia.org)
  • tissues
  • Cerebral organoids are synthesized tissues that contain several types of nerve cells and have anatomical features that resemble mammalian brains. (wikipedia.org)
  • Cell replacement therapy Cerebral organoids can be used as a simple model to show how cell replacement therapy would work on brain tissues. (wikipedia.org)
  • A bioreactor may also refer to a device or system meant to grow cells or tissues in the context of cell culture. (wikipedia.org)
  • Biology
  • 3 To whom correspondence should be addressed: Dept. of Physiology and Cell Biology, University of Nevada School of Medicine, 1664 N Virginia St., MS0575, Reno NV 89557. (pubmedcentralcanada.ca)
  • Numerous procedures in biology and medicine require the counting of cells. (wikipedia.org)
  • Protein crystallization has three major revenue-generating applications: structural biology and drug design, bioseparations, and controlled drug delivery. (wikipedia.org)
  • media
  • Customized to meet your specific application, our single-use technology systems include your choice of media, bag size, connectors, and tubing. (corning.com)
  • Enhance your cell culture applications with a broad range of cell culture media from Corning. (corning.com)
  • Cell counts within liquid media (such as blood, plasma, lymph, or laboratory rinsate) are usually expressed as a number of cells per unit of volume, thus expressing a concentration (for example, 5,000 cells per milliliter). (wikipedia.org)
  • The media used for a growing organ culture are generally the same as those used for tissue culture. (wikipedia.org)
  • Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick embryo extract and 3 parts of horse serum. (wikipedia.org)
  • A variety of adult organs (e.g. the liver) have been cultured using special media with special apparatus (Towell's II culture chamber). (wikipedia.org)
  • By alternating the pressure gradient across the hollow fiber membrane, media could flow back and forth between the EC side (cell compartment) and the IC side (hollow fiber lumen). (wikipedia.org)
  • This process, combined with the axial media flow created when media passes down the length of the fibers, optimized the growth environment throughout the entire bioreactor. (wikipedia.org)
  • Optimal IC and EC space perfusion rates must be achieved in order to efficiently deliver media nutrients and growth supplements, respectively, and to collect supernatant. (wikipedia.org)
  • During the cell growth phase within these bioreactors, the media feed rate is increased to accommodate the expanding cell population. (wikipedia.org)
  • Large scale immobilized cell bioreactors are: moving media, also known as moving bed biofilm reactor (MBBR) packed bed fibrous bed membrane Bioreactor design is a relatively complex engineering task, which is studied in the discipline of biochemical engineering. (wikipedia.org)
  • Certain compounds excreted by plant tissue cultures and found in culture media have been shown necessary to coordinate cell division and morphological changes. (wikipedia.org)
  • organs
  • The culture of adult organs or parts from adult animals is more difficult due to their greater requirement of oxygen. (wikipedia.org)
  • Research
  • Primary sources from the demand side include research scientists, cell culture specialists, laboratory technicians, and R&D professionals of pharmaceutical and biotechnology companies and academic research institutes. (pharmiweb.com)
  • The cell culture market is segmented into pharmaceutical and biotechnology companies and research institutes. (pharmiweb.com)
  • Create specialized cell culture environments to set your research up for success. (corning.com)
  • The research provides market segmentation based on types, applications, and geography. (medgadget.com)
  • Furthermore, consumption market share for each application is offered in the research for 2016 in a tabular format. (medgadget.com)
  • It is ideal for both research and large-scale culture systems utilizing serum-free or low-level serum applications. (sigmaaldrich.com)
  • LONG ® R 3 IGF-I is specifically designed and manufactured exclusively for the research and industrial cell culture market for distribution by SAFC Biosciences. (sigmaaldrich.com)
  • This has led to supply and availability issues for research and industrial cell culture users. (sigmaaldrich.com)
  • LONG ® R 3 IGF-I is an analogue of human-like growth factor I (IGF-I) specifically engineered for use in research and industrial cell culture. (sigmaaldrich.com)
  • It is an important subset of cytometry, with applications in research and clinical practice. (wikipedia.org)
  • The company is a provider of products and services that support scientists, clinical researchers, and physicians across basic research, translational research, and clinical applications. (wikipedia.org)
  • Microplates with an embedded layer of filter material were developed in the early 1980s by several companies, and today, there are microplates for just about every application in life science research which involves filtration, separation, optical detection, storage, reaction mixing, cell culture and detection of antimicrobial activity. (wikipedia.org)
  • In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganian's laboratory at the Boston Cancer Research Foundation and used it to derive the original Chinese hamster ovary (CHO) cell line. (wikipedia.org)
  • However these cells, when mutagenized, could revert to DHFR activity, making their utility for research somewhat limited. (wikipedia.org)
  • The fact that hollow fiber bioreactors are very small and yet enable incredibly high cell densities has led to their development for both research and commercial applications, including monoclonal antibody and influenza vaccine production. (wikipedia.org)
  • The focus is on biological research, development, and applications of significance to science and society. (wikipedia.org)
  • tissue cultures
  • Findings and inferences thus far indicate that there is a rich potential to explore new and potentially game-changing insights and applications in a range of areas, that includes but is not limited to protein crystallization, 3-D tissue cultures, tissue regeneration, DNA regulation, drug and vaccine development, stem cells, and treatments for diseases such as cancer and other life-threatening and debilitating conditions. (wikipedia.org)
  • vessels
  • Add appropriate aliquots of the cell suspension to new culture vessels. (atcc.org)
  • Using forced ventilation tubules within the culture vessels was developed after the idea of using the discs, as a way to ensure that uniform content of water vappor and Carbon dioxide is within each individual container. (wikipedia.org)
  • Bioreactor
  • A Hollow fiber bioreactor is a 3D cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. (wikipedia.org)
  • Cells are seeded into the EC space of the hollow fiber bioreactor and expand there. (wikipedia.org)
  • In addition, control of the fluid dynamics within each hollow fiber bioreactor led to further optimization of the cell culture environment. (wikipedia.org)
  • By incorporating EC cycling, the effects of Starling flow are eliminated and the entire bioreactor becomes nutrient-rich and optimized for cell growth. (wikipedia.org)
  • The environmental conditions inside the bioreactor, such as temperature, nutrient concentrations, pH, and dissolved gases (especially oxygen for aerobic fermentations) affect the growth and productivity of the organisms. (wikipedia.org)
  • signal transduction
  • The GH4C1 cell line provides a system for studying the molecular mechanisms of receptor modulation and signal transduction in pituitary - derived cells. (atcc.org)
  • flask
  • Add 2.0 to 3.0 mL of 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). (atcc.org)
  • functional
  • Experimental studies provide evidence of functional, histological, and electromyographical improvement following transplantation of bone-marrow-derived cells in animal models of peripheral nerve injury. (hindawi.com)
  • The experiment used rat heart cells and produced functional heart tissue. (wikipedia.org)
  • Biological
  • In laboratories, culture growth applications include controlled aerobic or anaerobic incubator atmospheres for biological cell culture or tissue growth. (wikipedia.org)
  • Similarly, NIH contends that the International Space Station (ISS) has the potential to further the study of: basic biological or behavioral mechanisms associated with maintaining health or developing disease, normal or pathological physiology and metabolism, and cell repair processes and tissue regeneration that occur naturally or are enhanced through medical interventions following injury or aging. (wikipedia.org)
  • The CHO and CHO-K1 cell lines can be obtained from a number of biological resource centres such as the European Collection of Cell Cultures (ECACC) which is part of the Health Protection Agency Culture Collections. (wikipedia.org)
  • 2016
  • Furthermore, current market trends and growth opportunities are discussed along with extensive analysis of each segment for the historic period, 2011-2016 and the forecast period, 2016-2021. (medgadget.com)
  • In 2016, another test was done in which human cells were used to assemble intricately structured hearts. (wikipedia.org)
  • adherent
  • The surfaces of these products are modified using an oxygen plasma discharge to make their surfaces more hydrophilic so that it becomes easier for adherent cells to grow on the surface which would otherwise be strongly hydrophobic. (wikipedia.org)
  • include
  • In addition to being used as tools to study disease pathology and treatments, future application of cerebral organoids include direct implantation into a host, like a human being. (wikipedia.org)
  • Examples of the need for cell counting include: In medicine, the concentration of various blood cells, such as red blood cells and white blood cells, can give crucial information regarding the health situation of a person (see: complete blood count). (wikipedia.org)
  • bone
  • MACS Technology is used to isolate cells from blood, bone marrow or dissociated tissue according to specific surface markers. (wikipedia.org)
  • widely
  • It is widely accepted that in CHO cells the effects of insulin are mediated by the IGF-IR, due to the fact there are relatively few IR present on the CHO cells and that insulin must be present at a high, non-physiological concentration typically 1-10 mg/L, to be effective. (sigmaaldrich.com)
  • Due to this, CHO cells lacking DHFR are the most widely used CHO cells for industrial protein production. (wikipedia.org)
  • The growth rate and the level of recombinant protein production of each cell line vary widely. (wikipedia.org)
  • Successfully
  • FBS contains a complex array of protein components that are required by many cells to grow which is why it has been successfully used in cell culture. (wikipedia.org)
  • Silk cut from silkworm cocoons has been successfully used as growth scaffolding for heart tissue production. (wikipedia.org)
  • Together with Prof Yoshihiro Ito from RIKEN, he has successfully pioneered a new method of cell detachment using a novel temperature-sensitive biomaterial. (wikipedia.org)
  • microorganisms
  • Studies that examine the growth rate of microorganisms (in other words: how fast they divide to create new cells) require cell counting. (wikipedia.org)
  • Under optimum conditions, the microorganisms or cells are able to perform their desired function with limited production of impurities. (wikipedia.org)