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  • progenitor
  • Isolated, adult progenitor / stem cell populations that express CD34 or CD133 markers on their surface have been used for cellular therapy in clinical settings. (wikipedia.org)
  • They are classified as either totipotent (iTC), pluripotent (iPSC) or progenitor (multipotent-iMSC, also called an induced multipotent progenitor cell-iMPC) or unipotent-(iUSC) according to their developmental potential and degree of dedifferentiation. (wikipedia.org)
  • adherent cells
  • The surfaces of these products are modified using an oxygen plasma discharge to make their surfaces more hydrophilic so that it becomes easier for adherent cells to grow on the surface which would otherwise be strongly hydrophobic. (wikipedia.org)
  • neural
  • Below is an image showing some of the chemical factors that can lead stem cells to differentiate into various neural tissues. (wikipedia.org)
  • Neural glial cells cover a wide variety of neural cells, some of which move around the neurons. (wikipedia.org)
  • Cell fate potential Cross species developmental timing Cerebral organoids provide a unique insight into the timing of development of neural tissues and can be used as a tool to study the differences across species. (wikipedia.org)
  • One example is the transformation of iris cells to lens cells in the process of maturation and transformation of retinal pigment epithelium cells into the neural retina during regeneration in adult newt eyes. (wikipedia.org)
  • Assays
  • Designed with a generous growth area of 500cm 2 to allow for larger assays and better overall use of space in incubators. (fishersci.com)
  • Certificates of Analysis vary between commercial suppliers, but each usually includes the following details: filtration statement, country of origin, cell growth performance testing, microbial sterility testing, screening for mycoplasma and virus, endotoxin, haemoglobin, IgG and total protein assays. (wikipedia.org)
  • Migration Assays Cerebral organoids can help to study cell migration. (wikipedia.org)
  • Cell-type specific genome assays Organoids can be used to study the crucial early stages of brain development, test drugs and, because they can be made from living cells, study individual patients. (wikipedia.org)
  • 2017
  • In January 2017, scientists from Salk Institute for Biological Studies managed to create a pig embryo that had part of its DNA, critical for the growth of organs, edited out. (wikipedia.org)
  • Miltenyi Biotec
  • Miltenyi Biotec technologies are used in applications concerned with accessing, analyzing, and utilizing primary and primary-derived cells - across basic research, translational research, and clinical applications. (wikipedia.org)
  • With a market share of 70%, Miltenyi Biotec is the leader in the field of magnetic cell separation. (wikipedia.org)
  • Miltenyi Biotec has expanded its portfolio to include a novel microchip-based cell sorting technology with the acquisition of Santa Barbara-based Owl biuomedical, Inc. in April 2013. (wikipedia.org)
  • grown
  • In one case, a cerebral organoid grown from a patient with microcephaly demonstrated related symptoms and revealed that apparently the cause is overly rapid development, followed by slower brain growth. (wikipedia.org)
  • this makes them the preferred counting method in fast biological experiments in which it needs to be merely determined whether a cell culture has grown as expected. (wikipedia.org)
  • The plasmid DNA carrying the two genes is then transfected into cells, and the cells are grown under selective conditions in thymidine-lacking medium. (wikipedia.org)
  • factors
  • The high growth in the Asian market is attributed to factors such as favorable regulatory guidelines, lower manufacturing costs, growing presence of market players in emerging economies, and favorable government investments in the life sciences sector. (pharmiweb.com)
  • This is due to it having a very low level of antibodies and containing more growth factors, allowing for versatility in many different cell culture applications. (wikipedia.org)
  • Serum is stored frozen to preserve the stability of components such as growth factors. (wikipedia.org)
  • Stem cells have the potential to grow into many different types of tissues and their fate is dependent on many factors. (wikipedia.org)
  • Furthermore, they do not always induce cellular transformation, and are not the only growth factors that induce cellular transformation. (wikipedia.org)
  • The researchers were able to identify the minimal conditions and factors that would be sufficient for starting the cascade of molecular and cellular processes to instruct pluripotent cells to organize the embryo. (wikipedia.org)
  • Likewise, because hollow fiber bioreactors use up significantly less medium and growth factors than traditional cell culture methods such as stirred-tank bioreactors, they offer a significant cost savings. (wikipedia.org)
  • tissues
  • Cerebral organoids are synthesized tissues that contain several types of nerve cells and have anatomical features that resemble mammalian brains. (wikipedia.org)
  • Cell replacement therapy Cerebral organoids can be used as a simple model to show how cell replacement therapy would work on brain tissues. (wikipedia.org)
  • A bioreactor may also refer to a device or system meant to grow cells or tissues in the context of cell culture. (wikipedia.org)
  • media
  • Customized to meet your specific application, our single-use technology systems include your choice of media, bag size, connectors, and tubing. (corning.com)
  • Recovery Cell Culture Freezing Medium is meant for general cell culture applications where a wide range of growth media will be used. (thermofisher.com)
  • Cell counts within liquid media (such as blood, plasma, lymph, or laboratory rinsate) are usually expressed as a number of cells per unit of volume, thus expressing a concentration (for example, 5,000 cells per milliliter). (wikipedia.org)
  • The media used for a growing organ culture are generally the same as those used for tissue culture. (wikipedia.org)
  • Media solidified with agar are also used for organ culture and these media consist of 7 parts 1% agar in BSS, 3 parts chick embryo extract and 3 parts of horse serum. (wikipedia.org)
  • A variety of adult organs (e.g. the liver) have been cultured using special media with special apparatus (Towell's II culture chamber). (wikipedia.org)
  • By alternating the pressure gradient across the hollow fiber membrane, media could flow back and forth between the EC side (cell compartment) and the IC side (hollow fiber lumen). (wikipedia.org)
  • This process, combined with the axial media flow created when media passes down the length of the fibers, optimized the growth environment throughout the entire bioreactor. (wikipedia.org)
  • Optimal IC and EC space perfusion rates must be achieved in order to efficiently deliver media nutrients and growth supplements, respectively, and to collect supernatant. (wikipedia.org)
  • During the cell growth phase within these bioreactors, the media feed rate is increased to accommodate the expanding cell population. (wikipedia.org)
  • Large scale immobilized cell bioreactors are: moving media, also known as moving bed biofilm reactor (MBBR) packed bed fibrous bed membrane Bioreactor design is a relatively complex engineering task, which is studied in the discipline of biochemical engineering. (wikipedia.org)
  • organs
  • The culture of adult organs or parts from adult animals is more difficult due to their greater requirement of oxygen. (wikipedia.org)
  • mammalian
  • CHO cells are the most commonly used mammalian hosts for industrial production of recombinant protein therapeutics. (wikipedia.org)
  • Genetics of somatic mammalian cells. (wikipedia.org)
  • Similar to a mammalian lung, the gas exchange cartridge efficiently oxygenated the culture medium, allowing the bioreactor to support higher numbers of cells. (wikipedia.org)
  • bone marrow
  • Experimental studies provide evidence of functional, histological, and electromyographical improvement following transplantation of bone-marrow-derived cells in animal models of peripheral nerve injury. (hindawi.com)
  • MACS Technology is used to isolate cells from blood, bone marrow or dissociated tissue according to specific surface markers. (wikipedia.org)
  • medium
  • We recommend using DMEM plus 10% FBS and 10% DMSO, or the Recovery Cell Culture Freezing Medium. (thermofisher.com)
  • Can Recovery Cell Culture Freezing Medium undergo multiple freeze thaw cycles? (thermofisher.com)
  • Can Recovery Cell Culture Freezing Medium be stored at 2 to 8°C? (thermofisher.com)
  • Recovery Cell Culture Freezing Medium should be stable at 2 to 8°C for 1 week, although we have no data to support this. (thermofisher.com)
  • Is it important to centrifuge and aspirate Recovery Cell Culture Freezing Medium before seeding into the flask? (thermofisher.com)
  • We recommend that you aspirate Recovery Cell Culture Freezing Medium before seeding, although other protocols can be substituted (this will need to be determined by the end user). (thermofisher.com)
  • Prior to staining with trypan blue Solution, do the cells need to be washed to remove the culture medium? (thermofisher.com)
  • If the background is too dark, cells should be pelleted and resuspended in protein-free medium, buffer, or normal saline prior to counting. (thermofisher.com)
  • The techniques for organ culture can be classified into (i) those employing a solid medium and (ii) those employing liquid medium. (wikipedia.org)
  • The medium with agar provides the mechanical support for organ culture. (wikipedia.org)
  • Cell lines with mutated DHFR are useful for genetic manipulation as cells transfected with a gene of interest along with a functional copy of the dhfr gene can easily be screened for in thymidine-lacking medium. (wikipedia.org)
  • One of the cell populations is fed with growth medium containing normal amino acids. (wikipedia.org)
  • In contrast, the second population is fed with growth medium containing amino acids labeled with stable (non-radioactive) heavy isotopes. (wikipedia.org)
  • Pulsed SILAC (pSILAC) is a variation of the SILAC method where the labelled amino acids are added to the growth medium for only a short period of time. (wikipedia.org)
  • Cell culture medium is pumped through the IC space and delivers oxygen and nutrients to the cells via hollow fiber membrane perfusion. (wikipedia.org)
  • As the cells expand, their waste products and CO2 also perfuse the hollow fiber membranes and are carried away by the pumping of medium through the IC space. (wikipedia.org)
  • As waste products build up due to increased cell mass, the rate of medium flow can also be increased so that cell growth is not inhibited by waste product toxicity. (wikipedia.org)
  • research
  • Primary sources from the demand side include research scientists, cell culture specialists, laboratory technicians, and R&D professionals of pharmaceutical and biotechnology companies and academic research institutes. (pharmiweb.com)
  • The cell culture market is segmented into pharmaceutical and biotechnology companies and research institutes. (pharmiweb.com)
  • Create specialized cell culture environments to set your research up for success. (corning.com)
  • Using Nunc Glass Bottom Dishes in your research applications gives you the flexibility to convert your culture device into an imaging device. (fishersci.com)
  • It is an important subset of cytometry, with applications in research and clinical practice. (wikipedia.org)
  • The company is a provider of products and services that support scientists, clinical researchers, and physicians across basic research, translational research, and clinical applications. (wikipedia.org)
  • Microplates with an embedded layer of filter material were developed in the early 1980s by several companies, and today, there are microplates for just about every application in life science research which involves filtration, separation, optical detection, storage, reaction mixing, cell culture and detection of antimicrobial activity. (wikipedia.org)
  • In 1957, Theodore T. Puck obtained a female Chinese hamster from Dr. George Yerganian's laboratory at the Boston Cancer Research Foundation and used it to derive the original Chinese hamster ovary (CHO) cell line. (wikipedia.org)
  • However these cells, when mutagenized, could revert to DHFR activity, making their utility for research somewhat limited. (wikipedia.org)
  • The fact that hollow fiber bioreactors are very small and yet enable incredibly high cell densities has led to their development for both research and commercial applications, including monoclonal antibody and influenza vaccine production. (wikipedia.org)
  • The focus is on biological research, development, and applications of significance to science and society. (wikipedia.org)
  • apoptosis
  • Analogous to the effect of erythropoietin (EPO), the primary mode of action of TPO is inhibition of apoptosis of its target cells. (miltenyibiotec.com)
  • HCAECs
  • HIPER inhibited TNF- α induced monocyte adhesion to HCAECs that was in keeping with decreased expression of vascular cell adhesion molecule-1 and intercellular cell adhesion molecule-1 and decreased nuclear factor-kappa B (NF- κ B) activation. (hindawi.com)
  • bioreactors
  • Suspension bioreactors can use a wider variety of organisms, since special attachment surfaces are not needed, and can operate at much larger scale than immobilized cultures. (wikipedia.org)
  • cellular
  • Cancer cells exhibit altered cellular functions compared to the normal functioning, non-malignant cells from which they are derived. (corning.com)
  • Interleukin 22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF), is a member of the IL-10 family which consists of potent mediators of cellular inflammatory response. (miltenyibiotec.com)
  • It can be applied to basically all types of biocatalysis including enzymes, cellular organelles, animal and plant cells. (wikipedia.org)
  • Biological
  • In laboratories, culture growth applications include controlled aerobic or anaerobic incubator atmospheres for biological cell culture or tissue growth. (wikipedia.org)
  • Similarly, NIH contends that the International Space Station (ISS) has the potential to further the study of: basic biological or behavioral mechanisms associated with maintaining health or developing disease, normal or pathological physiology and metabolism, and cell repair processes and tissue regeneration that occur naturally or are enhanced through medical interventions following injury or aging. (wikipedia.org)
  • The CHO and CHO-K1 cell lines can be obtained from a number of biological resource centres such as the European Collection of Cell Cultures (ECACC) which is part of the Health Protection Agency Culture Collections. (wikipedia.org)
  • Migration
  • A key early step in atherosclerotic lesion formation is the adhesion of monocytes to the endothelium and the subsequent migration of the monocytes into the subintima where they engulf oxidized LDL and become classical "foam cells" [ 3 ]. (hindawi.com)
  • Successfully
  • FBS contains a complex array of protein components that are required by many cells to grow which is why it has been successfully used in cell culture. (wikipedia.org)
  • Silk cut from silkworm cocoons has been successfully used as growth scaffolding for heart tissue production. (wikipedia.org)
  • Together with Prof Yoshihiro Ito from RIKEN, he has successfully pioneered a new method of cell detachment using a novel temperature-sensitive biomaterial. (wikipedia.org)
  • somatic
  • Induced stem cells (iSC) are stem cells derived from somatic, reproductive, pluripotent or other cell types by deliberate epigenetic reprogramming. (wikipedia.org)
  • Induced totipotent cells can be obtained by reprogramming somatic cells with somatic-cell nuclear transfer (SCNT). (wikipedia.org)
  • The process involves sucking out the nucleus of a somatic (body) cell and injecting it into an oocyte that has had its nucleus removed Using an approach based on the protocol outlined by Tachibana et al. (wikipedia.org)
  • microorganisms
  • Studies that examine the growth rate of microorganisms (in other words: how fast they divide to create new cells) require cell counting. (wikipedia.org)
  • Under optimum conditions, the microorganisms or cells are able to perform their desired function with limited production of impurities. (wikipedia.org)
  • include
  • In addition to being used as tools to study disease pathology and treatments, future application of cerebral organoids include direct implantation into a host, like a human being. (wikipedia.org)
  • Examples of the need for cell counting include: In medicine, the concentration of various blood cells, such as red blood cells and white blood cells, can give crucial information regarding the health situation of a person (see: complete blood count). (wikipedia.org)
  • Antibodies
  • Cell separation technology based on super paramagnetic beads was invented by the company founder, Stefan Miltenyi, in 1989 and enables the separation of any cell type using antibodies bound to magnetic beads. (wikipedia.org)
  • Bioreactor
  • A Hollow fiber bioreactor is a 3D cell culturing system based on hollow fibers, which are small, semi-permeable capillary membranes arranged in parallel array with a typical molecular weight cut-off (MWCO) range of 10-30 kDa. (wikipedia.org)
  • Cells are seeded into the EC space of the hollow fiber bioreactor and expand there. (wikipedia.org)
  • In addition, control of the fluid dynamics within each hollow fiber bioreactor led to further optimization of the cell culture environment. (wikipedia.org)
  • By incorporating EC cycling, the effects of Starling flow are eliminated and the entire bioreactor becomes nutrient-rich and optimized for cell growth. (wikipedia.org)
  • The environmental conditions inside the bioreactor, such as temperature, nutrient concentrations, pH, and dissolved gases (especially oxygen for aerobic fermentations) affect the growth and productivity of the organisms. (wikipedia.org)
  • genome
  • Surviving cell will have the exogenous DHFR gene along with the gene of interest integrated in its genome. (wikipedia.org)
  • development
  • KCa3.1 is highly expressed in a variety of nonexcitable and proliferating cells ( 2 ), and an increase in KCa3.1 expression has been associated with cancer development, immune disorders, and vascular inflammation. (pubmedcentralcanada.ca)
  • Findings and inferences thus far indicate that there is a rich potential to explore new and potentially game-changing insights and applications in a range of areas, that includes but is not limited to protein crystallization, 3-D tissue cultures, tissue regeneration, DNA regulation, drug and vaccine development, stem cells, and treatments for diseases such as cancer and other life-threatening and debilitating conditions. (wikipedia.org)
  • There are also potential applications for new drug development. (wikipedia.org)
  • Cell culture Drug development Pre-clinical development Wurm FM (2004). (wikipedia.org)
  • In 1924 Spemann and Mangold demonstrated the key importance of cell-cell inductions during animal development. (wikipedia.org)
  • contamination
  • This cell sorting technology is a major advancement in the field, allowing researchers to process small to large-scale samples in a gentle, efficient manner - all in a sterile environment that keeps users safe from potentially harmful samples and protects cells from contamination. (wikipedia.org)
  • substrate
  • 4. The process of claim 1, in which the methylotrophic and/or methanotrophic microorganism has been cultured on a substrate comprising as the primary carbon and/or energy source a compound which is partly (compared to a carbohydrate) or fully reduced. (patentsencyclopedia.com)
  • yeast
  • The white material is made up of debris, fibrin, and desquamated epithelium that has been invaded by yeast cells and hyphae that invade to the depth of the stratum spinosum. (wikipedia.org)
  • For single-celled eukaryotes, such as yeast, the isolation of pure cultures uses the same techniques as for bacterial cultures. (wikipedia.org)
  • proliferation
  • of TRPC decreased the cell routine S stage and cell migration, implicating an operating function for TRP-mediated Ca2+ entrance in cell proliferation and invasion. (cancercurehere.com)
  • Exogenous PUFA and a TRPC3 antagonist regularly attenuated breasts cancer tumor cell proliferation and migration, recommending a mechanism where PUFA restrains the breasts cancer partially via its inhibition of TRPC stations. (cancercurehere.com)
  • Ca2+ entrance via turned on TRPC was improved when PUFA had been absent, recommending a double-gating system for Danshensu TRPC which may be involved with MCF breasts cancer tumor cell proliferation and invasion. (cancercurehere.com)
  • Platelet-rich plasma was effective in stimulating cell proliferation and extracellular matrix metabolism. (blogspot.com)
  • fungus
  • Indeed, one of the main pests which can cause the collapse of the Haematococcus Pluvialis culture is a fungus. (wikipedia.org)
  • The fungus isolated from this cantaloupe produced several hundred times as much penicillin as Fleming's original cultures. (wikipedia.org)
  • citation needed] This discovery, in combination with vastly improved methods of culturing the fungus based on the principle of aerating the culture medium, resulted in the ability to mass-produce penicillin in quantities great enough for distribution and mass use in the United States Army, and also in the British army during the World War II. (wikipedia.org)
  • tissue
  • Laboratory research studies using tissue cultured cell lines have shown enhancement of the benzodiazepine binding site after chronic treatment with flumazenil where sites have become more numerous and uncoupling/down-regulation of GABAA has been reversed. (wikipedia.org)
  • For example, a throat culture is taken by scraping the lining of tissue in the back of the throat and blotting the sample into a medium to be able to screen for harmful microorganisms, such as Streptococcus pyogenes, the causative agent of strep throat. (wikipedia.org)
  • oxygen
  • Anaemia is a condition in which the blood does not have enough healthy red blood cells to transport oxygen to the body's organs. (indiatimes.com)
  • 12. The process of claim 1, wherein the culturing of the strain of the family Streptococcaceae is carried out under aeration, e.g. the culture is aerated so as to maintain an oxygen content which is at least 2 micromoles per liter of culture medium. (patentsencyclopedia.com)
  • Heterotrophic growth has only been observed, however, in the presence of oxygen. (wikipedia.org)
  • These cultures are not shaken and they provide the microbes with an oxygen gradient. (wikipedia.org)
  • Part of the reason for this growth could be related to studies that suggest cellulose may actually protect a building from damage in a fire better than fiberglass because cellulose is denser and restricts the oxygen necessary to burn structural members. (wikipedia.org)
  • neural
  • Thus, in X. laevis embryos, the ectoderm is patterned by the secretion of BMP antagonists from the dorsal blastopore lip, or Spemann's organizer: BMP signalling is active ventrally, and induces the formation of surface ectoderm, whereas dorsal BMP signalling is inhibited, and the cells adopt a neural fate ( Hemmati-Brivanlou and Melton, 1997 ). (biologists.org)
  • Designed for dramatically improved transfection efficiency, Invitrogen Lipofectamine Stem Transfection Reagent offers a simple, robust and reproducible method for delivering DNA, RNA and RNP such as Cas9/gRNA complexes into a wide range of stem cells, including pluripotent stem cells, neural stem cells and mesenchymal stem cells (Figure 2) . (thermofisher.com)
  • fungi
  • This fungi is a specific parasite for Haematococci cells. (wikipedia.org)
  • Results showed that there are special proteins (lectins) present on the zeospheres of the fungi which recognize specific sugar moieties upon the algal cell wall. (wikipedia.org)
  • transplantation
  • Volumetric analyses over large areas reveal that spectrally coded HSPC-derived cells can be detected non-invasively in various intact tissues, including the bone marrow (BM), for extensive periods of time following transplantation. (jove.com)
  • reagent
  • Cell routine evaluation After removal of methanol, cells had been treated having a Coulter DNA-Prep reagent package (Beckman-Coulter, France). (cancercurehere.com)
  • The goal of this study was to illustrate the versatility of Invitrogen Lipofectamine Stem Transfection Reagent, which was developed specifically for stem cells, to co-deliver multiple payloads in addition to large plasmid constructs with high transfection efficiency. (thermofisher.com)
  • This newest addition to the Lipofectamine transfection reagent family helps ensure high-efficiency transfection while maintaining maximum cell viability and growth in an undifferentiated state. (thermofisher.com)
  • (A) Human ES (H9) cells or Human Episomal iPSCs were transfected with Lipofectamine Stem Reagent or FuGENE HD Regent according to the accompanying protocol and either a 5 kb or 11 kb DNA plasmid expressing GFP and examined for GFP expression 24 hours posttransfection. (thermofisher.com)
  • In the bottom panel NCRM-iPS cells transfected using Lipofectamine Stem Reagent with a ~10.5 kb plasmid expressing Cas9 and GFP followed by detection via immunocytochemistry. (thermofisher.com)
  • (B) Human ES (H9) cells were transfected with Lipofectamine Stem Reagent or FuGENE HD Reagent according to the protocol with an mRNA expressing GFP. (thermofisher.com)
  • C, left) Lipofectamine Stem Reagent was used to transfect cells with Cas9 mRNA (modified)/ gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified) or Cas9 protein/gRNA: Emx-1 crRNA (Exon2)-tracrRNA oligo/ GFP mRNA (modified). (thermofisher.com)
  • biomass
  • 9. The process of claim 1, wherein the nutrient medium, in addition to the biomass of the methylotrophic and/or methanotrophic microorganism, contains biomass (such as whole cells, an extract or a lysate) obtained from at least one of the following species: Ralstonia sp. (patentsencyclopedia.com)
  • plasmids
  • Plasmids also include a strong polyadenylation/transcriptional termination signal, such as bovine growth hormone or rabbit beta-globulin polyadenylation sequences. (wikipedia.org)
  • agar
  • The sexual stage of P. chrysogenum was discovered in 2013 by mating cultures in the dark on oatmeal agar supplemented with biotin, after the mating types (MAT1-1 or MAT1-2) of the strains had been determined using PCR amplification. (wikipedia.org)
  • For the purpose of gelling the microbial culture, the medium of agarose gel (agar) is used. (wikipedia.org)
  • When the selected antibiotic is added to the agar, only bacterial cells containing the gene insert conferring resistance will be able to grow. (wikipedia.org)
  • Stab cultures are similar to agar plates, but are formed by solid agar in a test tube. (wikipedia.org)
  • humans
  • However, the discovery did not attract much attention until the 1940s, when Howard Florey, Norman Heatley, and Ernst Chain developed methods for mass production and application in humans, incited by the urgent war-time need for antibacterial agents. (wikipedia.org)
  • research
  • Within this research, we discovered the functional appearance of TRPC3 in individual MCF-7 breasts cancer tumor cell-mediated Ca2+ entrance. (cancercurehere.com)
  • Microbial cultures are foundational and basic diagnostic methods used extensively as a research tool in molecular biology. (wikipedia.org)
  • Microbial culture collections focus on the acquisition, authentication, production, preservation, catalogueing and distribution of viable cultures of standard reference microorganisms, cell lines and other materials for research in microbial systematics. (wikipedia.org)
  • successfully
  • Combining two-photon microscopy with fluorescent tracers, we successfully visualized the engrafted tissues at the single cell level in live mice over several months. (jove.com)
  • fate
  • Genetic combinatorial marking using lentiviral vectors encoding fluorescent proteins (FPs) enabled cell fate mapping through advanced microscopy imaging. (jove.com)
  • Vectors
  • Vectors encoding five different FPs: Cerulean, EGFP, Venus, tdTomato, and mCherry were used to concurrently transduce HSPCs, creating a diverse palette of color marked cells. (jove.com)
  • essential
  • During spermatogenesis in mammals and in Drosophila melanogaster, male germ cells develop in a series of essential developmental processes. (jove.com)
  • Human
  • The aim of this study is to estimate the influence of general environmental conditions on human organism at the final stage of the Neolithic period - in the Corded Ware culture. (blogspot.com)
  • Her son, the Sage of the Six Paths, was born with the ability to produce chakra by combining physical energy (身体エネルギー, shintai enerugī), drawn from the trillions of cells that make up the human body, and mental energy (精神エネルギー, seishin enerugī), gained through experience and meditation. (wikipedia.org)
  • These range from the hepatoxic brevianamide F (cyclo(L-Trp-L-Pro)) to the annulated tremorogenic verruculogen and the spiro-annulated spirotryprostatin B which represent a promising class of antimitotic arrest agents, to the structurally complex (+)-stephacidin A, a bridged 2,5-diketopiperazine that possess a unique bicyclo[2.2.iazaoctane core ring system and is active against the human colon HCT-116 cell line. (wikipedia.org)
  • Components
  • Imaging using confocal/two-photon hybrid microscopy enables simultaneous high resolution assessment of uniquely marked cells and their progeny in conjunction with structural components of the tissues. (jove.com)
  • production
  • The production of this pigment in Haematococcus Pluvialis is enhanced due to various environmental stresses which limit the growth of the cell under light conditions. (wikipedia.org)
  • population
  • Using a previously established mouse ES-cell-based system that recapitulates the development of the ectoderm lineage we have identified a transient population that is consistent with definitive ectoderm. (biologists.org)
  • and predicts formation of a cell population equivalent to the definitive ectoderm. (biologists.org)
  • shown
  • This was the first time that more than a single origin of DNA replication had been shown to be used in a prokaryotic cell. (wikipedia.org)
  • host cells
  • Because these proteins are recognised as foreign, when they are processed by the host cells and displayed on their surface, the immune system is alerted, which then triggers immune responses. (wikipedia.org)
  • Virus or phage cultures require host cells in which the virus or phage multiply. (wikipedia.org)
  • various
  • Whales have been depicted in various cultures worldwide, notably by the Inuit and the coastal peoples of Vietnam and Ghana, who sometimes hold whale funerals. (wikipedia.org)
  • whole cells
  • Due to advancements in recent years, biocatalysis through isolated enzymes is considered more economical than use of whole cells. (wikipedia.org)
  • Whole cells are typically used when a reaction requires a co-factor. (wikipedia.org)
  • specific
  • While killed vaccines do not have this risk, they cannot generate specific killer T cell responses and may not work at all for some diseases. (wikipedia.org)
  • Furthermore, the term culture is more generally used informally to refer to "selectively growing" a specific kind of microorganism in the lab. (wikipedia.org)
  • sugar
  • The word cellulose comes from the French word cellule, for a living cell, and glucose, which is sugar. (wikipedia.org)
  • plasma
  • GH secretion and plasma insulin-like growth factor-I levels were reduced in female Tg rats. (jci.org)
  • The response to platelet-rich plasma was greater in the case of anulus fibrosus cells than of nucleus pulposus cells. (blogspot.com)
  • In this download probabilistic models for nonlinear partial differential equations, we are extended evidence climate of Nel, by providing a plasma of cell effects for collaborative areas of the Nel study. (pd19.org)
  • addition
  • In addition, post-meiotic germ cells undergo a dramatic morphological reshaping process as well as a global epigenetic reconfiguration of the germ line chromatin-the histone-to-protamine switch. (jove.com)
  • individual
  • Two-photon microscopy has enabled the study of individual cell behavior in live animals. (jove.com)
  • Four dimensional (three-dimensional (3D) plus time) information of individual cells was obtained from this imaging. (jove.com)
  • uses
  • The DNA is injected into cells, whose "inner machinery" uses the DNA to synthesize the proteins. (wikipedia.org)
  • produce
  • DNA vaccination is a technique for protecting against disease by injection with genetically engineered DNA so cells directly produce an antigen, producing a protective immunological response. (wikipedia.org)
  • similar
  • It is also sometimes used to reverse the effects of benzodiazepines after surgery in a manner similar to naloxone's application to reverse the effect of opiates and opioids following surgery. (wikipedia.org)
  • Analysis
  • Recognition and analysis of these transient cell populations has been hampered by technical difficulties in accessing them in vivo. (biologists.org)
  • conditions
  • Industrial biological catalysis through enzymes has experienced rapid growth in recent years due to their ability to operate at mild conditions, and exceptional chiral and positional specificity, things that traditional chemical processes lack. (wikipedia.org)
  • focus
  • Additionally, our outcomes also claim that TRPC3 shows up as a fresh mediator of breasts cancer tumor cell migration/invasion and represents a potential focus on for a fresh course of anticancer agent. (cancercurehere.com)
  • variety
  • It has also been isolated from a variety of marine microorganisms and has been identified as an active LasI quorum-sensing signal molecule important for the plant growth promotion by Pseudomonas aeruginosa. (wikipedia.org)
  • development
  • He is involved in the development and application of multifunction control/display switch technology in 1983 and Development and application of a microprocessor-based I/O system for simulator use in 1984. (wikipedia.org)
  • life
  • I am a strong advocate of the potential of Web 2.0 and social learning to empower learners from all walks of life and cultures. (blogspot.fr)
  • active
  • Although co-factors may be generated in vitro, it is typically more cost-effective to use metabolically active cells. (wikipedia.org)
  • sample
  • Microbial cultures are used to determine the type of organism, its abundance in the sample being tested, or both. (wikipedia.org)