HepatomaApoptosisApoptoticDifferentiationViabilityProliferationHumansInhibitorsHepatocellular carcinomaToxicityVitroEndothelial cellsEpithelialInfectionAcuteLineArrestTumor growthFunctionalProteinsDensitiesPathwayMammalianAssaySecreteLung CancerSignificantlyLiver cancerPromotesTrialsProteinSerumConditionsEffectsVirusOrganismStudySuspensionCultureStudiesPrimarilyExpression
Hepatoma6
- Another well-characterized polarized hepatocyte cell line is the rat hepatoma-derived hybrid cell line WIF-B. With the proper culture conditions, Hep G2 cells display robust morphological and functional differentiation with a controllable formation of apical and basolateral cell surface domains (van IJzendoorn et al. (wikipedia.org)
- In view of these considerations, this study was designed to evaluate the cytotoxic and genotoxic potential of geraniol, in an in vitro protocol, using two types of human cells: one without the ability to metabolize (peripheral blood mononuclear cells - PBMC), and the other with this capability (human hepatoma cell line - HepG2) through the comet assay and the micronucleus test. (geneticsmr.org)
- In this study, we examined the antitumor effects of GS-002 in human hepatoma Hep3B and HepG2 cells in vitro . (hindawi.com)
- Here, we investigated the effect of arecoline on the proliferation and migration of human HepG2 hepatoma cells and its potential oncogenic mechanisms. (biomedcentral.com)
- We further investigated the roles of these predicted markers by treating human hepatoma HepG2 cultures with arecoline. (biomedcentral.com)
- Cytotoxicity, antiproliferative and apoptosis-inducing capacity of ACNVs against hepatoma carcinoma cell were assessed. (dovepress.com)
Apoptosis10
- These include regulation of cell cycle, activation of mitochondrial pathway, induction of mitochondrial outer membrane permeabilization, induction of apoptosis, modulation of oxidative stress, amelioration of inflammation, modulation of insulin signaling and inhibition of angiogenesis. (mdpi.com)
- Diminution of p53 by RNA interference induced necrosis instead of apoptosis in A549 cells following terpinen-4-ol treatment, indicating that terpinen-4-ol-elicited apoptosis is p53-dependent. (hindawi.com)
- Collectively, these data provide insight into the molecular mechanisms underlying terpinen-4-ol-induced apoptosis in NSCLC cells, rendering this compound a potential anticancer drug for NSCLC. (hindawi.com)
- Recent reports have indicated that terpinen-4-ol exerts its antitumor effects by triggering caspase-dependent apoptosis in human melanoma cells or by inducing necrotic cell death and cell-cycle arrest in mouse mesothelioma and melanoma cell lines without affecting normal cells [ 14 , 15 ]. (hindawi.com)
- Our results indicated that terpinen-4-ol induced apoptosis through a mitochondria-mediated pathway in NSCLC cells and that the apoptosis elicited by terpinen-4-ol was p53 dependent. (hindawi.com)
- Activating transcription factor-(ATF-) 3, a stress-inducible transcription factor, is rapidly upregulated under various stress conditions and plays an important role in inducing cancer cell apoptosis. (hindawi.com)
- First, we found that GS-002 significantly inhibited cell proliferation and induced cell apoptosis in dose-dependent manners. (hindawi.com)
- Furthermore, we found that GS-002 induced more cell apoptosis in ATF-3-overexpressing cells. (hindawi.com)
- ACNVs were found to possess specific antitumor cell proliferation activity associated with an apoptosis-inducing pathway. (dovepress.com)
- Treatment with oridonin at 44 μM for 24 h induced apoptosis and G2/M cell cycle arrest, which were associated with nine differentially expressed proteins identified by proteomic analysis. (edu.hk)
Apoptotic4
- Increased sub-G1 population and annexin-V binding, activation of caspases 9 and 3, cleavage of poly(ADPribose) polymerase (PARP), and a decrease of mitochondrial membrane potential (MMP) indicated involvement of the mitochondrial apoptotic pathway in terpinen-4-ol-treated A549 and CL1-0 cells. (hindawi.com)
- Several main apoptotic indicators were found in GS-002-treated cells, such as the cleaved forms of caspase-3, caspase-9, and poly(ADP-ribose) polymerase (PARP). (hindawi.com)
- Up-regulation of Hsp70.1, STRAP, TCTP, Sti1 and PPase, as well as the down-regulation of hnRNP-E1 could be responsible for the apoptotic and G2/M-arresting effects of oridonin observed in this study. (edu.hk)
- As part of toxicity studies, localization of SNP in Hep G2 cell line, cell viability, biochemical effects and apoptotic/necrotic potential were assessed. (nih.gov)
Differentiation2
- Because of their high degree of morphological and functional differentiation in vitro, Hep G2 cells are a suitable model to study the intracellular trafficking and dynamics of bile canalicular, sinusoidal membrane proteins, and lipids in human hepatocytes in vitro. (wikipedia.org)
- S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. (cancerindex.org)
Viability2
- However, caution is advised in the use of this substance by humans, since a significant reduction in viability of HepG2 and a marked decrease in cell viability on normal PBMC were verified. (geneticsmr.org)
- Cell viability assay showed that oridonin dose-dependently inhibited cell growth with an IC 50 of 41.77 μM. (edu.hk)
Proliferation5
- The article also underscores the various possible mechanisms by which honey may inhibit growth and proliferation of tumors or cancers. (mdpi.com)
- Additionally, miR-146a silencing or restoration of FLAP led to a reduction of HepG2 cell proliferation, cell cycle progression, migration, and invasion. (biomedcentral.com)
- ATF-3 induces DNA synthesis and expression of cyclin D1 in hepatocytes [ 21 ] and is involved in serum-induced cell proliferation as a target gene of c-myc [ 10 ]. (hindawi.com)
- Compared with control group, 2.5 µM arecoline treatment increased the proliferation and migration ability of the HepG2 cells. (biomedcentral.com)
- A low concentration arecoline can induce the proliferation and migration of HepG2 cells, with the potential mechanism of action linked to high levels of exosomal miR-21 and miR-1267, activation of the PI3K-AKT pathway, upregulation of CDK1 and CCND1, and downregulation of RAF1. (biomedcentral.com)
Humans1
- In humans, acute hepatitis infection caused by HEV is a serious public health concern in developing countries. (cdc.gov)
Inhibitors1
- The endocytosis mechanism of ACNVs was evaluated on Hep G2 cells in the presence of different endocytosis inhibitors. (dovepress.com)
Hepatocellular carcinoma3
- The Hep G2 cell line has been isolated from a liver biopsy of a male Caucasian aged 15 years, with a well differentiated hepatocellular carcinoma. (sigmaaldrich.com)
- Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma. (wikipedia.org)
- We demonstrated that miR-146a expression is overexpressed, while FLAP protein and mRNA are suppressed in hepatocellular carcinoma tissues and HepG2 cells compared to para-carcinoma tissues and HL-7702 cells. (biomedcentral.com)
Toxicity2
- citation needed] Hep G2 cells and their derivatives are also used as a model system for studies of liver metabolism and toxicity of xenobiotics, the detection of environmental and dietary cytotoxic and genotoxic (and thus cytoprotective, anti-genotoxic, and cogenotoxic) agents, understanding hepatocarcinogenesis[citation needed], and for drug targeting studies[citation needed]. (wikipedia.org)
- In vivo antitumor activity and toxicity were evaluated in Hep G2 cell xenograft model. (dovepress.com)
Vitro1
- citation needed] Hep G2 cells are a suitable in vitro model system for the study of polarized human hepatocytes. (wikipedia.org)
Endothelial cells1
- MiR-146a has also been shown to promote the angiogenic activity of endothelial cells in HCC patients [ 11 ]. (biomedcentral.com)
Epithelial1
- These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice. (wikipedia.org)
Infection4
- The progress of HCC is complex and multifactorial caused by pathogenic factors such as chronic infection with Hepatitis B virus (HBV) or Hepatitis C virus (HCV), liver cirrhosis, habitual alcohol abuse, and aflatoxin B1 exposure [ 3 ]. (biomedcentral.com)
- The major risk factors include chronic hepatitis B virus (HBV) infection, chronic hepatitis C virus (HCV) infection, environmental carcinogens such as aflatoxin B1 (AFB1), alcoholic cirrhosis, and inherited genetic disorder such as hemochromatosis, Wilson's disease, and tyrosinemia. (hindawi.com)
- therefore, hepatitis E caused by G3 and G4 HEV infection is recognized as a zoonosis ( 3 ). (cdc.gov)
- To further investigate the potential susceptibility of rats to infection with human HEV, we experimentally injected nude rats with G3 HEV and monitored virus growth. (cdc.gov)
Acute1
- The cells secrete a variety of major plasma proteins, e.g., albumin, and the acute-phase proteins fibrinogen, alpha 2-macroglobulin, alpha 1-antitrypsin, transferrin and plasminogen. (wikipedia.org)
Line4
- Hep G2 cell line human has been used to study the cytotoxicity of carbon monoxide-releasing molecules and organic solvents on HepG2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides (AEPS) for HepG2 using the MTT colorimetric assay. (sigmaaldrich.com)
- Please note a wild-type cell line is not automatically included with a knockout cell line order, if required please add recommended wild-type cell line at no additional cost using the code WILDTYPE-TMTK1. (abcam.com)
- Hep G2 (or HepG2) is a human liver cancer cell line. (wikipedia.org)
- DQ079632) and 1 derived from the supernatant of a hepatocarcinoma cell line, PLC/PRF/5, that was injected with the pig specimen. (cdc.gov)
Arrest1
- GS-002 also induced endoplasmic reticular (ER) stress as evidenced by increases in ER stress-responsive proteins including glucose-regulated protein 78 (GRP78), growth arrest- and DNA damage-inducible gene 153 (GADD153), phosphorylated eukaryotic initiation factor 2 α (eIF2 α ), phosphorylated protein endoplasmic-reticular-resident kinase (PERK), and ATF-3. (hindawi.com)
Tumor growth2
- Furthermore, treatment of s.c xenografts derived from A549 cells with intratumor injections of terpinen-4-ol significantly inhibited tumor growth compared with the control group. (hindawi.com)
- In vivo antitumor results showed that PEGylated ACNVs could significantly inhibit tumor growth without side effects. (dovepress.com)
Functional1
- Extracellular vesicles (EVs) are a group of cell-derived membranous structures, which play an important role in intercellular communication and functional transduction. (dovepress.com)
Proteins2
- The cells secrete a variety of major plasma proteins e.g. albumin, α2-macroglobulin, α 1-antitrypsin, transferrin and plasminogen. (sigmaaldrich.com)
- This can be important for the study of human liver diseases that are caused by an incorrect subcellular distribution of cell surface proteins, e.g., hepatocanalicular transport defects such as Dubin-Johnson Syndrome and progressive familial intrahepatic cholestasis (PFIC), and familial hypercholesterolemia. (wikipedia.org)
Densities1
- All seeding densities should be based on cell counts gained by established methods. (abcam.com)
Pathway2
- In breast cancer, ATF-3 enhances cancer cell-initiating features [ 22 ] and is associated with activation of the canonical Wnt/ β -catenin pathway [ 23 ]. (hindawi.com)
- These DE-miRNAs, hub genes and pathway were proved in arecoline-treated HepG2 cells. (biomedcentral.com)
Mammalian1
- 1 , 2 In recent years, mammalian cell-derived exosomes (a type of extracellular vesicle) have been widely used in the field of biomedicine, including diagnosis, regulation of disease progression and drug delivery. (dovepress.com)
Assay1
- Four concentrations (10, 25, 50, and 100 μg/mL) were selected for the genotoxic assessment for PBMC and three (1.25, 2.5, and 5 μg/mL) for HepG2 cells based on cytotoxicity tests (MTT assay). (geneticsmr.org)
Secrete1
- 3 , 4 Similarly, plant cells also secrete exosome-like nanovesicles (ELNVs) that can participate in various physiological processes such as the inheritance of traits and defense response. (dovepress.com)
Lung Cancer3
- In this study, the antitumor effects of terpinen-4-ol and the cellular and molecular mechanisms responsible for it were evaluated and studied, respectively on human nonsmall cell lung cancer (NSCLC) cells. (hindawi.com)
- Among lung cancers, nonsmall cell lung carcinomas (NSCLC) account for approximately 80% of lung cancer cases [ 1 ]. (hindawi.com)
- In addition, there is no report on the antitumor effects of terpinen-4-ol against human nonsmall cell lung cancer cells. (hindawi.com)
Significantly1
- Moreover, intratumoral administration of terpinen-4-ol significantly suppressed the growth of s.c. (hindawi.com)
Liver cancer2
- Herein, we aimed to demonstrate the expression of miR-146a and FLAP in human HCC tissues and liver cancer cell lines. (biomedcentral.com)
- These findings shed new insights into the molecular mechanisms underlying the anticancer properties of oridonin in liver cancer cells. (edu.hk)
Promotes1
- This study showed that miR-146a has a stimulatory role in HepG2 cells and promotes HepG2 cell migration and invasion by targeting FLAP mRNA. (biomedcentral.com)
Trials1
- Hep G2 cells are also employed in trials with bio-artificial liver devices[citation needed]. (wikipedia.org)
Protein2
- Abnormal expression of 5-Lipoxygenase Activating Protein (FLAP) has been detected in many tumor cells. (biomedcentral.com)
- The induction of ATF-3 expression was mediated by mitogen-activated protein kinase (MAPK) signaling pathways in GS-002-treated cells. (hindawi.com)
Serum3
- Cell Freezing Medium-DMSO Serum free media, contains 8.7% DMSO in MEM supplemented with methyl cellulose. (abcam.com)
- The A549 human lung adenocarcinoma and CL1-0 lung adenocarcinoma cell lines were cultured in Dulbecco′s modified eagle medium supplemented with 10% fetal bovine serum (FBS) and 1% antibiotic antimycotic. (hindawi.com)
- On the other hand, ATF-3 is rapidly induced in cells treated with growth stimulators such as serum and growth factors [ 20 ]. (hindawi.com)
Conditions1
- Results showed that geraniol did not present genotoxic or clastogenic/aneugenic effects on both cell types under the conditions studied. (geneticsmr.org)
Effects3
- These effects of honey have been thoroughly investigated in certain cancers such as breast, liver and colorectal cancer cell lines. (mdpi.com)
- Therefore, in this study, the anticancer effects of terpinen-4-ol were evaluated on two NSCLC cell lines, namely, A549 and CL1-0 human lung adenocarcinoma cells. (hindawi.com)
- Rather, its genotoxic effects are limited to indirect clastogenicity occurring in select cell types, and only when high dose and time thresholds are exceeded. (cdc.gov)
Virus2
- Hepatitis B virus surface antigens have not been detected. (sigmaaldrich.com)
- Hepatitis E virus (HEV) is a positive-sense single-stranded RNA virus (genus Hepevirus , family Hepeviridae ) ( 1 ). (cdc.gov)
Organism1
- The post agent effect (a parameter measuring the length of time for which bacterial growth remains suppressed following brief exposure to the antimicrobial agent) varied with the type of organism (e.g., 10.5 h for P. aeruginosa, 1.3 h for Staphylococcus sp. (nih.gov)
Study1
- recently reported that G1, G2, and G3 do not infect laboratory rats ( 7 ), and we found in a previous study that laboratory rats are not susceptible to G1, G3, or G4 HEV ( 8 ). (cdc.gov)
Suspension1
- 2. Transfer the cell suspension (0.8 mL) to a 15 mL/50 mL conical sterile polypropylene centrifuge tube containing 8.4 mL pre-warmed culture medium, wash vial with an additional 0.8 mL culture medium (total volume 10 mL) to collect remaining cells, and centrifuge at 201 x g (rcf) for 5 minutes at room temperature. (abcam.com)
Culture3
- 3. Resuspend the cell pellet in 5 mL pre-warmed culture medium and count using a haemocytometer or alternative cell counting method. (abcam.com)
- Based on cell count, seed cells in an appropriate cell culture flask at a density of 2x10 4 cells/cm 2 . (abcam.com)
- the 3 rats in group 2 were injected with the cell culture supernatant sample, which contained 4 × 10 6 copies of G3 HEV. (cdc.gov)
Studies1
- Previous studies reported that ATF-3 was induced by treating cells with antitumorigenic compounds [ 14 - 18 ] and a phosphoinositide 3-kinase inhibitor [ 19 ]. (hindawi.com)
Primarily1
- Sickle cell anemia, which primarily affects Black or African American people, is associated with a shorter life span and life-threatening complications that can affect all parts of the body. (cdc.gov)
Expression1
- Knockdown of miR-146a also resulted in increased FLAP expression of cancer cells. (biomedcentral.com)