Grown epithelial cells. Medical search. Frequent questions

Significant downregulation of β-defensin1 gene ( DEFB1 ) expression was observed when human bronchial epithelial cells (HBEpCs) were exposed to IAV. (cdc.gov)
Primary human bronchial epithelial cells HBEp cells. (cdc.gov)
To determine whether FP reduces cytokine production in bronchial epithelial cells via NF-κB, the authors investigated the nonstimulated and the Pseudomonas aeruginosa lipopolysaccharide (LPS) stimulated production of NF-κB-dependent interleukin (IL)-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) along with the activation of NF-κB in non-CF and CF human bronchial gland epithelial cells. (ersjournals.com)
It was demonstrated that a relevant concentration of FP (10 −8 M) inhibited constitutive and P. aeruginosa LPS-induced IL-6 and IL-8 production of non-CF and CF bronchial epithelial cells. (ersjournals.com)
Interestingly, the expression of two IκB kinases (IKK)-α/β, the degradation of cytosolic IκB-β inhibitor and the NF-κB deoxyribonucleic acid binding activity were markedly reduced after FP treatment in both CF and non-CF bronchial epithelial cells. (ersjournals.com)
It was shown by the authors that fluticasone propionate exerts an anti-inflammatory effect by blocking a signal transduction leading to a reduced level of IκB-α/β kinases in bronchial epithelial cells. (ersjournals.com)
In particular the strong effect on the IκB-β kinase, which is known to be elevated in bronchial epithelial cells in cystic fibrosis patients, was observed. (ersjournals.com)
The present authors and previous studies, have previously demonstrated that epithelial expression of interleukin (IL)-8 is excessively elevated in the bronchial epithelial cells of human CF adults 6 . (ersjournals.com)
Normal human bronchial epithelial cells (NHBE) were grown in culture and exposed to hog confinement dust extract. (cdc.gov)
In primary cultures of human bronchial epithelial cells (HBECs) with 16.6±0.4 mM glucose in the basolateral medium, ASL glucose was 2.2±0.5 mM [ 11 ]. (ersjournals.com)

The primary barriers for invading respiratory pathogens are the airway epithelial cells of the respiratory tract and antimicrobial peptides produced by these cells. (cdc.gov)
In this study, we utilized primary normal human bronchial epithelial (NHBE) cells that are cultured at an air-liquid interface (ALI) to mimic the in vivo airway characteristics 30 . (nature.com)
Inhaled fluticasone propionate (FP) is widely used to reduce pulmonary inflammation in chronic obstructive pulmonary disease, but the potential effects of FP on airway epithelial cells from patients with cystic fibrosis (CF) are unknown. (ersjournals.com)
In CF disease, a nonregulated inflammatory lung response occurs through exaggerated nuclear factor (NF)-κB activation and elevated pro-inflammatory cytokines production by airway epithelial cells. (ersjournals.com)
Cystic fibrosis (CF) is a lethal inherited disorder caused by mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) in airway epithelial cells 1 . (ersjournals.com)
One of the major clinical manifestations of CFTR mutations is neutrophil dominated inflammation 2 , 3 , implying that defective CFTR affects the immune function of airway epithelial cells, in particular the production of nuclear factor (NF)-κB-dependent chemokines. (ersjournals.com)
How CFTR dysfunction in CF airway cells contributes to the activation and nuclear localisation of NF-κB is still unclear. (ersjournals.com)
Hog confinement dust upregulated airway epithelial cell TLR2 mRNA in a concentration- and time-dependent manner using real-time PCR. (cdc.gov)
TLR2 was upregulated on the surface of airway epithelial cells as shown by flow cytometry. (cdc.gov)
IL-6 alone was able to upregulate TLR2 in airway epithelial cells. (cdc.gov)
In this study, we investigated the effect of hog confinement dust exposure on expression of TLR2 mRNA and protein in an in vitro model of airway epithelial cells and in an in vivo murine model of hog confinement dust exposure. (cdc.gov)
In immortalised human airway cells (line H441) with 10 mM glucose in the basolateral medium, apical ASL glucose was 0.24±0.07 mM [ 10 ]. (ersjournals.com)
Differential gene regulation in human small airway epithelial cells grown in monoculture versus coculture with human microvascular endothelial cells following multiwalled carbon nanotube exposure. (cdc.gov)

Using RNA-Seq, we identified a total of 163 and 568 differentially expressed genes in primary normal human bronchial epithelial (NHBE) cells that were exposed to diacetyl and 2,3-pentanedione, respectively. (nature.com)

The results revealed that hyperforin inhibited IL‑13‑induced nasal epithelial inflammatory cytokine release and repressed the damage to the cellular barrier from IL‑13 stimulation. (spandidos-publications.com)

In summary, the present results revealed that hyperforin suppressed IL‑13‑induced nasal epithelial cell inflammation and barrier damage by targeting BCL6/p38 MAPK/CCL11, which may provide promising therapeutic targets for AR. (spandidos-publications.com)
However, whether hyperforin is involved in nasal epithelial cell damage caused by AR is unknown. (spandidos-publications.com)
Thus, the present study aimed to explore the functional roles of hyperforin on IL-13-stimulated nasal epithelial cells and discuss the mechanism by which hyperforin influences nasal epithelial cell viability, inflammation and barrier damage. (spandidos-publications.com)

We have shown that AUF1 induces EMT and stemness in breast epithelial cells via stabilization of the SNAIL1 and TWIST1 mRNAs, and their consequent upregulation. (nature.com)
Normally, the PVRL4 gene is not active in breast epithelial cells, but its activity is detected in many breast, lung, and ovarian tumors. (elifesciences.org)

Recently, an in vitro study on the expression of ΔF508 CFTR (CFTR which contains a deletion of phenylalanine at position 508) in the ovary cells of Chinese hamsters, which do not express CFTR, revealed a seven-fold increase in the activation of NF-κB compared to the wild-type CFTR or the G551D mutant. (ersjournals.com)
Martin's research is focused on the nanoparticle-(mammalian)cell interaction, with a view to determining the mechanistic toxicological, immunological and genotoxic effects that nanoparticles, with varying physico-chemical characteristics, may cause at the cellular level by using advanced, next-level in vitro systems combined with state-of-the-art microscopy and bioanalytical approaches. (swansea.ac.uk)
100 publications within the field of nanotoxicology and the nanoparticle-cell interaction in vitro . (swansea.ac.uk)
In vitro characterization revealed that the strains tolerate gastric and bile challenges and display a great adhesion capacity to human intestinal cells. (frontiersin.org)
In vitro studies in resistive human lung epithelial cell monolayers grown at an air-liquid interface have supported these in vivo observations. (ersjournals.com)
Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)
In this report, we show that infection with influenza A virus and treatment with the resulting host signals (febrile-range temperatures, norepinephrine, extracytoplasmic ATP, and increased nutrient availability) induce the release of bacteria from biofilms in a newly developed biofilm model on live epithelial cells both in vitro and during in vivo colonization. (lu.se)
Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
Dive into the research topics of 'Role of serotype and virulence determinants of Streptococcus pyogenes biofilm bacteria in internalization and persistence in epithelial cells in vitro. (lu.se)
Here we develop and apply the principles of their evolution from systems principally for evaluating mechanism dosimetry in vitro and outline an approach for simulation of of toxicity to high-throughput systems for rapid and cost-effective nanoparticle particokinetics in cell culture systems. (cdc.gov)

To better understanding respiratory pathogen host- cell interactions in the airways, one approach is to instead grow and differentiate these cells at an air -liquid interface (ALI). (bvsalud.org)
By adding three genes, they converted those cells into induced pluripotent stem cells (iPSCs), which have the capability to differentiate into any cell types in the body. (scienceblog.com)
2003). Similarly, when transplanted into ischemic cortical areas, they were found to differentiate into 'neuron-like' cells (Okawa et al. (scielo.org.ar)
To differentiate between epithelial mesothelioma and the other forms, doctors will rely on histology - or more specifically, histopathology. (jeffreysglassman.com)
SHED was able to differentiate into epithelial like cells when cultured in keratinocyte growth medium (KGM) 2 . (bvsalud.org)

In an effort to advance the treatment strategies for the ocular surface diseases, I am focused on a regenerative medicine and single cell RNA sequencing based approaches to better comprehend the cellular biomarkers and molecular mechanisms involved in corneal tissue development and diseases. (stanford.edu)
Specular microscopy demonstrated an abnormal corneal endothelium with low cell counts OS. (uiowa.edu)
Abnormal corneal endothelial cells act like epithelial cells and may grow into the angle to block trabecular meshwork outflow. (uiowa.edu)
METHODS The experimental samples were obtained from the culture media of conjunctival and corneal epithelial cells, from fractionated blood plasma and leucocytes of healthy subjects and patients with rheumatoid arthritis, and from the tears of healthy subjects and patients with a variety of anterior segment diseases. (bmj.com)

Consistent with this, qRT-PCR confirmed that the expression of multiple genes involved in cilia biogenesis was significantly downregulated by diacetyl and 2,3-pentanedione in NHBE cells. (nature.com)
The effects of corticosteroids are predominantly mediated by the inhibition of NF-κB deoxyribonucleic acid (DNA)-binding activity, which controls genes encoding inflammatory cytokines, chemokines and adhesion molecules in respiratory epithelial cells 10 - 13 . (ersjournals.com)
What's more, the process Xu used to create iPSCs involves genetic modification of human cells with genes encoding oncogenic proteins and so needs more refinement. (scienceblog.com)
For example, the genes BRCA1 and BRCA2 normally keep cancer cells from forming by repairing or destroying cells with damaged DNA. (smartimagebase.com)
Mutations to these genes allow damaged cells to live and continue proliferating, resulting in the formation of cancerous tumors. (smartimagebase.com)
A gain of function screen for genes that enable proliferation independently of matrix anchorage identified a cell adhesion molecule PVRL4 (poliovirus-receptor-like 4), also known as Nectin-4. (elifesciences.org)
explored which genes enable epithelial cells from the human mammary gland to grow without being attached to the extracellular matrix. (elifesciences.org)
The total chromosomal content of a cell involves approximately 105 genes in a specialized macromolecule of deoxyribonucleic acid (DNA). (cdc.gov)

Respiratory epithelial cells fail to exhibit natural phenotypic and morphological characteristics when grown in standard cell culture conditions. (bvsalud.org)
Increasing evidence suggests that respiratory epithelial cells with dysfunctional CFTR have exaggerated activation of NF-κB/inhibitor of NF-κB complex associated with upregulated expression of chemokines 4 , 5 . (ersjournals.com)
ASL glucose concentrations are the net result of diffusion of glucose from blood and interstitial fluid across the respiratory epithelium into the ASL, and removal of glucose from ASL by epithelial glucose transport processes. (ersjournals.com)
Coronaviruses are enveloped, positive-stranded RNA analysis of the consequences of virus replication in Vero viruses associated with enteric and respiratory diseases in cells. (cdc.gov)
Here, live respiratory epithelial cells were inoculated with broth-grown or biofilm bacteria of different M-types, as well as with isogenic mutants lacking common virulence factors. (lu.se)

This image shows a single cell grown from a culture of lung epithelial cancer cells. (livescience.com)
For over 50 years, glucose has been recognised to cross the lung epithelial barrier and be transported by lung epithelial cells. (ersjournals.com)
Since the mid-1960s, it has been known that there are energy-dependent, sodium-coupled glucose transporter (SGLT) and energy-independent, facilitative glucose transporter (GLUT) pathways for glucose uptake in the lung [ 1 ], and that glucose can permeate the alveolar epithelial barrier [ 2 ]. (ersjournals.com)
Most often epithelial cancer cells will look like adenocarcinoma, which is a type of lung cancer. (jeffreysglassman.com)
Lung epithelial cells are located on the surface of the internal parts of the lung, and they are responsible for gas exchange (i.e., breathing). (lu.se)
These cells are heavily damaged in patients with lung fibrosis. (lu.se)
Previously, our research team had found that two molecules, or more specifically, co-transcriptional regulators, known as YAP and TAZ, are involved in the damage of the lung epithelial cells. (lu.se)
Identification of Prognostic and Chemopredictive microRNAs for Non-Small-Cell Lung Cancer by Integrating SEER-Medicare Data. (cdc.gov)

Prominent among the molecular adhesion components are integrins (β1 and β2), that mediate the interactions of a variety of immune cells to extracellular matrices, and to other immune cells, respectively. (weizmann.ac.il)
Binding of IgA prevented fungal epithelial adhesion and invasion resulting in a dampened proinflammatory epithelial response. (frontiersin.org)
Modelling the role of membrane mechanics in cell adhesion on titanium oxide nanotubes. (ki.si)
We now provide evidence that the observed apical epithelial packing minimizes surface energy at the intercellular apical adhesion belt. (biorxiv.org)

Currently we are collecting and growing epithelial cells from the ocular surface of donor eye tissues. (stanford.edu)
The knowledge gained from separating and identifying different cells types of the eye will provide greater understanding into the physiology and overall function of the various eye tissues. (stanford.edu)
Even though the frequencies of the different polygon types differ widely between different epithelial tissues, all epithelia analysed to date indeed meet this topological constraint in that their cells have on average close to six neighbours ( Fig. 1d,e ). (biorxiv.org)
These specialized cells absorb nutrients and are a critical barrier that prevent pathogens from spreading to deeper tissues. (sciencedaily.com)
As the National Society for Histotechnology notes, histology is the science that deals with the structure of cells and their formation into tissues and organs. (jeffreysglassman.com)
A single-cell layer of epithelium is separated from the tissues beneath it by a supporting substance called the extracellular matrix. (elifesciences.org)
Understanding how cancerous cells gain this ability may lead to new approaches to stopping tumor cells from dividing and colonizing tissues around the body. (elifesciences.org)
This behavior is particularly evident in the cells that divide aggressively to form tumors that subsequently migrate and colonize other tissues around the body. (elifesciences.org)
These cells can harm healthy tissues in your body and begin to spread to other parts of your body. (knowbreastcancer.org)
Notch-1, Jagged-1, Jagged-2, and stem cell marker Nanog are expressed in SHED cultured in KGM which may be involved in the differentiation into epithelial-like cells in human dental pulp tissues. (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) are multipotent stem cells derived from the pulp tissues of extracted deciduous teeth 1 . (bvsalud.org)
Notch signaling pathway is also involved in the regulation of epithelial cell differentiation in various tissues 5-6 . (bvsalud.org)

Human conjunctival epithelial cells in primary culture were infected with Ad19 at multiplicities of infection (MOI) of 0, 0.1 or 1.0 and harvested at 1.5h, 6h or 16h post-infection. (arvojournals.org)

Here, we have investigated the role of ChoK in the development of breast cancer and found that ChoK is both necessary and sufficient for growth factor-induced proliferation in primary human mammary epithelial cells and an absolute requirement for the specific mitogenic response to heregulin in breast tumor-derived cells. (aacrjournals.org)
These results demonstrate that ChoK plays an essential role in both normal human mammary epithelial cell proliferation and breast tumor progression. (aacrjournals.org)
In the synthetic niche, interactions with the functionalized scaffold are expected to provide the signals needed for T-cell activation (with or without antigen specificity), lineage selection, proliferation, and survival. (weizmann.ac.il)
Primary cultured HTFs were incubated with transforming growth factor β1 (TGF-β1) alone or combined with nintedanib, and cell proliferation and migration were measured by cell counting kit-8 (CCK8) and the scratch wound assay, respectively. (molvis.org)
The Notch signaling pathway provides important intercellular signaling mechanisms essential for cell fate specification and it regulates differentiation and proliferation of stem or progenitor cells by para-inducing effects 3-4 . (bvsalud.org)

One of the first lines of defense in the GI tract is the physical barrier provided by a single layer of intestinal epithelial cells. (sciencedaily.com)
However, in previous studies, NIAID scientists had observed that some Salmonella replicate rapidly in the cytosol -- the fluid portion -- of intestinal epithelial cells. (sciencedaily.com)
Then they infected laboratory mice with the self-destructing Salmonella bacteria and found that replication in the cytosol of mouse intestinal epithelial cells is important for colonization of the GI tract and fuels fecal shedding. (sciencedaily.com)

b , A typical epithelial tissue from the Drosophila larval eye disc peripodial epithelium (dPE), coloured according to the number of neighbours per cell. (biorxiv.org)
As noted by the U.S. National Library of Medicine , the epithelium refers to the layer of cells that line the hollow organs and glands. (jeffreysglassman.com)
Epidermal appendages serve an important role as a source of epithelial cells, which accomplish re-epithelialization when the overlying epithelium is removed or destroyed in cases such as partial-thickness burns, abrasions, or split-thickness skin graft harvesting. (medscape.com)
Together with mucus-secreting goblet cells within the stratified epithelium, melanocytic cells are found in the basal layer of the conjunctiva. (medscape.com)

Since epithelial-to-mesenchymal transition (EMT) and the acquisition of cancer stem cell traits are important for cancer onset and progression, we sought to determine the role of AUF1 in these two important processes. (nature.com)
Ectopic expression of AUF1 also promoted stemness in mammary epithelial cells, and thereby increased the proportion of cancer stem cells. (nature.com)
Together, these findings provide clear evidence that AUF1 is an important inducer of the EMT process through stabilization of SNAIL1 and TWIST1 and the consequent promotion of breast cancer stem cells. (nature.com)
Organoids are three-dimensional "mini organs" grown from stem cells. (acs.org)
Defined culture of human embryonic stem cells and xeno-free derivation of retinal pigmented epithelial cells on a novel, synthetic substrate. (ca.gov)
Human embryonic stem cells (hESCs) can generate an unlimited source of RPE for cellular therapies, and clinical trials have been initiated. (ca.gov)
In this study, we investigated the maintenance of hESCs and their differentiation into RPE using Synthemax II-SC, which is a novel, synthetic animal-derived component-free, RGD peptide-containing copolymer compliant with good manufacturing practices designed for xeno-free stem cell culture. (ca.gov)
Cornea is constantly regenerated from a small population of limbal epithelial stem cells via transiently amplifying cell (TAC) states. (stanford.edu)
Hematopoietic stem cell (HSC) replacement transplantation can ameliorate diseased hematologic system, thus inhibiting immune disorders including autoimmune diseases and immunodeficient disorders. (stanford.edu)
One potential approach to reversing hair loss uses stem cells to regenerate the missing or dying hair follicles. (scienceblog.com)
But it hasn't been possible to generate sufficient number of hair-follicle-generating stem cells - until now. (scienceblog.com)
As reported in a Penn news release , Xiaowei "George" Xu, MD, PhD, associate professor of Pathology and Laboratory Medicine and Dermatology at the Perelman School of Medicine, University of Pennsylvania, and colleagues published in Nature Communications a method for converting adult cells into epithelial stem cells (EpSCs), the first time anyone has achieved this in either humans or mice. (scienceblog.com)
The epithelial stem cells, when implanted into immunocompromised mice, regenerated the different cell types of human skin and hair follicles, and even produced structurally recognizable hair shaft, raising the possibility that they may eventually enable hair regeneration in people. (scienceblog.com)
They then converted the iPS cells into epithelial stem cells, normally found at the bulge of hair follicles. (scienceblog.com)
Starting with procedures other research teams had previously worked out to convert iPSCs into keratinocytes, Xu's team demonstrated that by carefully controlling the timing of the growth factors the cells received, they could force the iPSCs to generate large numbers of epithelial stem cells. (scienceblog.com)
In the Xu study, the team's protocol succeeded in turning over 25% of the iPSCs into epithelial stem cells in 18 days. (scienceblog.com)
Comparison of the gene expression patterns of the human iPSC-derived epithelial stem cells with epithelial stem cells obtained from human hair follicles showed that the team had succeeded in producing the cells they set out to make in the first place. (scienceblog.com)
This is the first time anyone has made scalable amounts of epithelial stem cells that are capable of generating the epithelial component of hair follicles," Xu says. (scienceblog.com)
That said, iPSC-derived epithelial stem cells are not yet ready for use in human subjects, Xu adds. (scienceblog.com)
First, a hair follicle contains epithelial cells - a cell type that lines the body's vessels and cavities - as well as a specific kind of adult stem cell called dermal papillae. (scienceblog.com)
Still, he notes that stem-cell researchers are developing more workarounds, including strategies using only chemical agents. (scienceblog.com)
Placental stem cells. (scielo.org.ar)
Aim: To detect the expression of molecules associated with Notch signaling pathway in stem cells from human exfoliated deciduous teeth (SHED) cultured in specific differentiation medium, namely, keratinocyte growth medium (KGM). (bvsalud.org)
RNA was reverse-transcribed to obtain the cDNA and then proceeded with PCR using specific primers for the Notch signaling pathway molecules (Notch1, Jagged-1, Jagged-2 and, Hes1) as well as stem cell marker (Nanog). (bvsalud.org)
Knowledge on the expression analysis of Notch signaling pathway molecules in SHED cultured in KGM could highlight its involvement in controlling the biological activity of these stem cells, particularly during odontogenesis and other developmental process. (bvsalud.org)
Stem cells from human exfoliated deciduous teeth (SHED) (ALLCells, Alameda, CA, USA) were employed in the current study. (bvsalud.org)

We previously reported host cell transcriptomic (mRNA) responses to an ocular isolate of Ad5 (see IOVS 46, 3657-3665, 2005), and now explore the responses to Ad19. (arvojournals.org)

Epithelial cell tumors- the most common type of ovarian cancer tumor. (mountsinai.org)
These tumors grow in the cells that line or cover the ovaries. (mountsinai.org)
Epithelial cell tumors may also develop in the fallopian tubes that carry the eggs produced in the ovaries to the uterus. (mountsinai.org)
Germ cell tumors- start in the ovarian cells that form eggs. (mountsinai.org)
Germ cell tumors tend to occur in young women or are in their reproductive years. (mountsinai.org)
Diagnosed at an early stage, germ cell tumors are curable. (mountsinai.org)
Sex cord-stromal cell tumors- begin in the connective cells that hold the ovaries in place and produce female hormones. (mountsinai.org)
Sex cord-stromal cell tumors tend to occur in women past menopause. (mountsinai.org)
Giant cell tumors of the tendon sheath are benign lesions. (aafp.org)
Giant cell tumors of the tendon sheath are most common in women 30 to 50 years of age. (aafp.org)
Schwannomas are rare, benign nerve tumors of the hand that present as painless, encapsulated, slow-growing nodules. (aafp.org)
As tumors invade the surrounding tissue, cells may break off into a lymphatic vessel. (smartimagebase.com)
Displaced tumor cells travel through the lymphatics and become metastatic tumors elsewhere. (smartimagebase.com)
The tumors are slow growing, in early stages majority are asymptomatic and are diagnosed incidentally. (orpha.net)
Furthermore, growth of orthotopically implanted tumors in vivo is inhibited by blocking PVRL4-driven cell-to-cell attachment with monoclonal antibodies, demonstrating a novel strategy for targeted therapy of cancer. (elifesciences.org)
It is estimated that up to 90% of cancers in humans originate in epithelial tissue, and the cells within such tumors are known to survive and divide even when they are no longer attached to the extracellular matrix. (elifesciences.org)
used genetic techniques to silence PVRL4 in cells from breast tumors, they found that it reduced the formation of clusters by the cancer cells and also reduced their ability to grow in the absence of attachment. (elifesciences.org)
If your glandular cells begin to change or grow out of control, tumors can form. (knowbreastcancer.org)
Some tumors found in glandular cells are not cancerous. (knowbreastcancer.org)
Some other rarer types of ovarian cancer are malignant germ cell tumors and stromal tumors. (medlineplus.gov)
The clinical differential diagnosis of a fibroma depends on its clinical presentation and location and includes giant cell fibroma, neurofibroma , peripheral giant cell granuloma , schwannoma, granular cell tumor, mucocele , and benign and malignant salivary gland tumors (eg, see Salivary Gland Neoplasms ). (medscape.com)
These melanocytic cells are of neuroectodermal origin, and melanocytic tumors may arise from these cells. (medscape.com)

Moreover, hyperforin promotes the expression of tight junction proteins in endothelial cells after blood-brain barrier injury ( 14 ). (spandidos-publications.com)
Porcine aortic endothelial cell, double-labeled for actin (green) and phospho-tyrosine (red). (weizmann.ac.il)

At this juncture, through the present study it was found that, chicken neural retinal cells when grown alone failed to survive and contrarily when either co-cultured with chicken amniotic epithelial cells / cultured in amniotic epithelial cell conditioned medium not only survived but also showed extensive differentiation. (scielo.org.ar)
Results showed that in cultured human-derived retinal pigment epithelial ARPE-19 cells , the extract of Chlorella zofingiensis and its nutritional ingredient astaxanthin exhibited significant inhibitory effects on the formation of endogenous N ε -carboxymethyllysine (CML), a key AGE representative, through the suppression of intracellular oxidative stress. (rsc.org)

The (13)C-isotopologue patterns of protein-derived amino acids from the bacteria and the host cells were then determined by mass spectrometry. (uni-muenchen.de)
In modern times, H. pylori has been disappearing, which consequently affects the interactions between luminal bacteria and epithelial, lymphoid, and neuroendocrine cells. (nih.gov)
When bacteria invade these cells, the cells are ejected into the gut lumen -- the hollow portion of the intestines. (sciencedaily.com)
That prompted them to ask: does ejecting the infected cell amplify rather than eliminate the bacteria? (sciencedaily.com)
To address this question, the scientists genetically engineered Salmonella bacteria that self-destruct when exposed to the cytosol of epithelial cells but grow normally in other environments, including the lumen of the intestine. (sciencedaily.com)
These dispersed bacteria have distinct phenotypic properties different from those of both biofilm and broth-grown, planktonic bacteria, with the dispersed population showing differential virulence gene expression characteristics resulting in a significantly increased ability to disseminate and cause infection of otherwise sterile sites, such as the middle ear, lungs, and bloodstream. (lu.se)
In this study, we determined that changes in the nasopharyngeal environment result in the release of bacteria from colonizing biofilms with a gene expression and virulence phenotype different not only from that of colonizing biofilm bacteria but also from that of the broth-grown planktonic bacteria commonly used for pathogenesis studies. (lu.se)
The M3 protein, but not the M1 or M5 proteins, was required for optimal uptake and persistence of both planktonic and biofilm bacteria inside cells. (lu.se)

Based on our theoretical framework, we explain how cell growth and division as well as mechanical constraints act together to drive epithelial tissue organization. (biorxiv.org)
These cells are square-shaped, and they grow right next to one another mostly via local expansion into surrounding tissue. (jeffreysglassman.com)
Histotechnology involves detecting abnormalities in the cells and employs the use of chemicals and dyes to produce colors and make it easier to distinguish different tissue structures. (jeffreysglassman.com)
Epithelial tissue is one of the four major types of tissue found in animals, and is the only type of tissue that is able to form and maintain layers of cells that are just one cell thick. (elifesciences.org)
These factors can contribute to adherence and multiplication at an epithelial surface, evasion of immune defense mechanisms or invasion into host cells and tissue. (lu.se)

Th2 cytokines also induce the breakdown of the epithelial barrier in allergic diseases, and epithelial cells release cytokines to promote Th2 immune response ( 6 ). (spandidos-publications.com)
FP induces apoptosis of eosinophils 18 and also reduces the production of several cytokines such as IL-1β, IL-6, IL-8 and RANTES (regulated on activation, T-cell expressed and secreted) by alveolar macrophages 19 and lymphocytes 20 , 21 . (ersjournals.com)
a2,3) Both DCs and T cells interact with FRCs and their secreted chemokines (CCL19, CCL21), as well as cytokines (IL-6, IL-7), which promote their migration and survival. (weizmann.ac.il)
In these studies, we tailor extracellular nanopatterns that can provide specific stimulation of cytokines, and reduce the rate of cell death. (weizmann.ac.il)

Like all cancers, breast cancer begins with cell DNA damage. (smartimagebase.com)
Most breast cancers form in duct epithelial cells. (smartimagebase.com)
While mesothelioma accounts for less than 2 percent of all known cancers, epithelial mesothelioma is the most common type of this malignancy. (jeffreysglassman.com)

Therefore, preservation of the epithelial barrier function can reduce the type 2 immune response caused by the influx of harmful environmental molecules, including allergens, which is considered as a promising therapeutic strategy for AR therapy ( 7 ). (spandidos-publications.com)
Cell-cell and cell-matrix adhesions are known to play key roles in the recruitment and activation of immune T-cells. (weizmann.ac.il)
In our current attempts to create a synthetic "immune niche," we employ diverse surface patterning approaches that might mimic "partnering cells", which are regularly located in the immune niches, and replace them in the process of T-cell activation (Figure 1). (weizmann.ac.il)
b) In a novel approach for construction of a synthetic immune niche, many of the cell-bound and ECM-associated ligands are transferred to a synthetic 2D (left) or 3D scaffold (right), to which T cells can bind. (weizmann.ac.il)
Bacterial cell wall components and toxins, for example, are well-known virulence factors for sepsis that directly contribute to the profound dysregulation of the immune response. (lu.se)

SARS-CoV-2 Infection of Human Primary Nasal Multiciliated Epithelial Cells Grown on Air-Liquid Interface Cultures. (bvsalud.org)
Using RNA-Seq, we performed global transcriptomic profiling in primary NHBE cells exposed to diacetyl or 2,3-pentanedione. (nature.com)
Our data showed that diacetyl and 2,3-pentanedione induced significant transcriptomic changes, including those related to ciliogenesis, in the primary NHBE cells. (nature.com)
Here, we use primary nasal epithelial cells (NECs) from children and adults, differentiated at an air-liquid interface to show that the ancestral SARS-CoV-2 replicates to significantly lower titers in the NECs of children compared to those of adults. (plos.org)
Our aim is to establish phenotypically and transcriptionally characterized different subsets of primary human ocular cells. (stanford.edu)
combining the primary Ad19 data with an additional experiment using cells grown in secondary culture revealed 18 panels with consistent, time-dependent changes. (arvojournals.org)
together with protein concentration) and lens epithelial cells plus capsule (LECs/capsule) in patients with cataract (CAT), and also suffering from pseudoexfoliation syndrome (PEX), primary open-angle glaucoma (POAG) and pseudoexfoliation glaucoma (PXG). (molvis.org)
The natural history of primary acquired melanosis begins with the development of superficial epithelial pigmentation, with a typical peppered pigment distribution. (medscape.com)

Carbon metabolism of enterobacterial human pathogens growing in epithelial colorectal adenocarcinoma (Caco-2) cells. (uni-muenchen.de)
Surprisingly, the metabolism of the host cells, as judged by the efficiency of (13)C-incorporation into host cell amino acids, was not significantly affected by the infection with either of these intracellular pathogens. (uni-muenchen.de)

Amniotic epithelial cells were believed to secrete an 'Epidermal Growth Factor (EGF) - like' factor and exact identification was not made. (scielo.org.ar)
During pregnancy, specialized secretory cells develop in the acini, and after birth, secrete milk. (smartimagebase.com)
These cells secrete mucous, digestive juices or other liquids. (knowbreastcancer.org)

These intradermal epithelial structures, such as sebaceous glands, sweat glands, and hair follicles, are lined with epithelial cells with the potential for division and differentiation. (medscape.com)
Since the Notch signaling pathway molecules play an important role in differentiation of epithelial cells, it is important to identify the presence of notch signaling molecules in SHED during the process of cell differentiation. (bvsalud.org)

In an adaptive immunity OPC rechallenge model using a derivate of the pathogenic C. albicans strain SC5314, CD4 + Th17 cells protect from mucosal Candida infection but can be compensated by other IL-17-producing cells in CD4-deficient hosts ( 15 , 16 ). (frontiersin.org)

Fungal colonization increased cross-specific IgA levels in the saliva and the tongue, and IgA + cells migrated to foci of fungal colonization. (frontiersin.org)
Besides CD19 + CD138 − B cells, plasmablasts, and plasma cells were enriched in the tongue of mice colonized with C. albicans suggesting a potential role of B lymphocytes during oral fungal colonization. (frontiersin.org)

When they mixed those cells with mouse follicular inductive dermal cells and grafted them onto the skin of immunodeficient mice, they produced functional human epidermis (the outermost layers of skin cells) and follicles structurally similar to human hair follicles. (scienceblog.com)
Xu and his team mixed iPSC-derived EpSCs and mouse dermal cells to generate hair follicles to achieve the growth of the follicles. (scienceblog.com)

In the present study, cell viability was analyzed using Cell Counting Kit‑8 assay, while inflammation was detected using ELISA and reverse transcription‑quantitative PCR. (spandidos-publications.com)
For instance, hyperforin can improve imiquimod-induced psoriasis-like skin inflammation in mice by regulating γδ T cells that produce IL-17A ( 13 ). (spandidos-publications.com)
The goal of the cornea ageing and rejuvenation project is to analyze the difference between a young adult versus an old adult in terms of their markers involved in ageing, senescence, inflammation, mitochrondria dysfunction and epithelial homeostasis. (stanford.edu)

Neuroendocrine tumor of the rectum is a rare epithelial tumor of rectum arising from enterochromaffin cells, most often in the mid-rectum. (orpha.net)

Epithelial cells are responsible to enclose and protect the organs, and most produce some type of secretion, such as mucus. (jeffreysglassman.com)
Adenocarcinoma develops in cells located in the glands that line your organs ( glandular epithelial cells ). (knowbreastcancer.org)
Adenocarcinoma happens when the cells in the glands that line your organs begin to grow out of control . (knowbreastcancer.org)

In this study, we analysed the intracellular carbon metabolism of enteroinvasive Escherichia coli (EIEC HN280 and EIEC 4608-58) and Salmonella enterica Serovar Typhimurium (Stm 14028) replicating in epithelial colorectal adenocarcinoma cells (Caco-2). (uni-muenchen.de)
The data showed that EIEC HN280 growing in the cytosol of the host cells, as well as Stm 14028 replicating in the Salmonella-containing vacuole (SCV) utilised glucose, but not glucose 6-phosphate, other phosphorylated carbohydrates, gluconate or fatty acids as major carbon substrates. (uni-muenchen.de)
The scientists hypothesize that, by hijacking the epithelial cell response, Salmonella amplify their ability to invade neighboring cells and seed the intestine for fecal shedding. (sciencedaily.com)

By virtue of expressions of glial and neural surface markers and capability of neurotransmitter metabolism, amniotic epithelial cells are considered as candidate cell type for transplantation strategies to treat neurological disorders. (scielo.org.ar)

The perturbances in cellular redox homeostasis leads to high free radical burden thereby causing oxidative stress in the cells. (stanford.edu)
When the genetic information containing the "blueprint" for these substances is disrupted, cell homeostasis is disrupted, resulting in a wide-range of immediate and/or delayed toxicological effects. (cdc.gov)

In addition, Ingenuity Pathway Analysis (IPA) integrated with PCR array data showed that the JAK1/STAT3 pathway was significantly altered by cells overexpressing DEFB1 , suggesting that this may be one of the pathways by which defensin regulates IAV replication in HBEpCs. (cdc.gov)
The expression levels of B‑cell lymphoma 6 (BCL6) and the p38 MAPK/C‑C motif chemokine 11 (CCL11) pathway were detected using western blotting. (spandidos-publications.com)

During all stages of tumor progression, cancer cells are subjected to inappropriate extracellular matrix environments and must undergo adaptive changes in order to evade growth constraints associated with the loss of matrix attachment. (elifesciences.org)

Protein collected from cells infected or uninfected using RIPA lysis buffer. (cdc.gov)
Each confocal microscope image here highlights a protein characteristic of a different type of cell in a human heart organoid from Aitor Aguirre's biomedical engineering lab at Michigan State University. (acs.org)
They found that the gene that codes for a protein called poliovirus receptor-like 4 (PVRL4) allows attachment-free cell growth and also makes cells cluster together once detached from the matrix. (elifesciences.org)
also showed that interactions between the PVRL4 in one cell and a related protein called PVRL1 in a neighboring cell were responsible for holding the cells together in clusters. (elifesciences.org)

If the ovaries develop abnormal cells that grow uncontrollably, it may be a type of gynecologic cancer. (mountsinai.org)
Abnormal endothelium with large spaces between cells and low cell count (1353). (uiowa.edu)
Cancer is a disease in which abnormal cells in the body grow out of control and form a tumor. (medlineplus.gov)
Breast cancer occurs when cells in the breast become abnormal and divide into more cells uncontrollably. (msdmanuals.com)

These findings suggested the usefulness of them as an alternate source of cells for transplantation approaches to treat neurological disorders (Sakuragawa et al. (scielo.org.ar)
Transplantation of amniotic epithelial cells in various regions of central nervous system such as caudate nucleus (Bankiewicz et al. (scielo.org.ar)
Amniotic epithelial cells transplantation in Parkinson model of rats was found to reverse the condition and prevent death in neurons (Kakishita et al. (scielo.org.ar)
2001). In our previous report, we concluded the usefulness of amniotic epithelial cells transplantation in spinal cord injury repair research. (scielo.org.ar)
We had also outlined various biological and social advantages by which, amniotic epithelial cells transplantation excels its precedent viz. (scielo.org.ar)
Recently, our conclusion of usefulness of amniotic epithelial cells transplantation to treat spinal cord injuries has been confirmed in rodent models of spinal cord injury (Zhi-yuan et al. (scielo.org.ar)

Zhen (Jason) He at the McKelvey School of Engineering used wastewater from University Dining Services to compare traditional algae-growing methods to his novel method, which includes extracting nutrients first, and then using them to grow algae outside of the water. (wustl.edu)

In this paper, we review and present our new data of LED treatment on cells grown in culture, on ischemic and diabetic wounds in rat models, and on acute and chronic wounds in humans. (researchgate.net)
This necessitates the investigation of past experimental approaches in their attempt to correlate oxidative stress and ocular diseases in humans in both the epithelial cells plus the capsule (LECs/capsule) and in the aqueous humor (AH). (molvis.org)

Furthermore, we aim at creating conditions that will enable us to develop an effective ex vivo stimulation system, tailored for different subclasses of T cells, including helper CD4 + cells, cytotoxic (CD8 + ) T cells, and T regulatory (T-Reg) cells. (weizmann.ac.il)

The study, published in Cell Host & Microbe , was conducted by National Institute of Allergy and Infectious Diseases (NIAID) scientists at Rocky Mountain Laboratories in Hamilton, Montana. (sciencedaily.com)

Large neoplastic epithelial cells with arge nucleus and light, glycogen-rich cytoplasm. (whonamedit.com)
Each cell (except for red blood cells) contains a nucleus that houses these chromosomes. (cdc.gov)

We hypothesize that iPSC reprogrammed from ocular epithelial cells will be more efficient in differentiating into ocular lineages and exhibit higher functionality than non-ocular sources such as fibroblasts or blood. (stanford.edu)
SHED has the ability to be differentiated to specific cell lineages such as odontoblasts and osteoblasts as well as epithelial like cells. (bvsalud.org)

Epithelial cell barrier damage was measured using western blotting and immunofluorescence staining. (spandidos-publications.com)
Epithelial cells play a key protective role, since they are the first physiological barrier against allergen infiltration ( 5 ). (spandidos-publications.com)

Epithelial cells growing on a patterned adhesive surface with the shape of the Weizmann Institute tree. (weizmann.ac.il)
Lewis' law emerges because the apical cell surfaces then assume the most regular polygonal shapes within a contiguous lattice, thus minimising the average perimeter per cell, and thereby surface energy. (biorxiv.org)
We will show that epithelial organization can be explained by a minimization of surface energy. (biorxiv.org)
Glucose diffusion is determined by epithelial permeability to glucose, the transepithelial glucose gradient and surface area. (ersjournals.com)
These are also the cells that comprise the outer surface of the body. (jeffreysglassman.com)
In contrast to soluble chemicals, cadmium oxide particles on a cm2/ml media basis, but are only ~50 particles can settle, diffuse, and aggregate differentially accord- times more potent when differences in delivery to adherent cells are ing to their size, density, and surface physicochemistry. (cdc.gov)

a4) Activated T-cells form intercellular contacts with each other and cluster through ICAM-1-LFA-1 binding. (weizmann.ac.il)

Epithelial mesothelioma - also sometimes referred to as epithelioid mesothelioma - is so named because it affects the epithelial cells. (jeffreysglassman.com)

New devices have demonstrated significant results for the treatment of medical conditions, including mild-to-moderate acne vulgaris, wound healing, psoriasis, squamous cell carcinoma in situ (Bowen's disease), basal cell carcinoma, actinic keratosis, and cosmetic applications. (researchgate.net)
Patient is status post excision of a recurrent basal cell carcinoma. (medscape.com)

Anatomy74

Organisms13

Diseases9

Chemicals and Drugs78

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes49

Disciplines and Occupations2

Information Science3

Bronchial epithelial cells10

Airway13

Normal human bronchial epithelial1

Induced nasal ep1

Nasal epithelial cell3

Breast epithelial cells2

Vitro11

Differentiate5

Corneal4

Genes8

Respiratory5

Lung8

Adhesion4

Tissues12

Conjunctival1

Proliferation5

Intestinal3

Epithelium4

Stem26

MRNA1

Tumors22

Endothelial2

Retinal2

Bacteria8

Tissue5

Cytokines4

Cancers3

Immune5

Primary8

Pathogens2

Secrete3

Differentiation2

Infection1

Colonization2

Hair follicles2

Inflammation3

Neuroendocrine1

Organs3

Salmonella3

Metabolism1

Homeostasis2

Pathway2

Tumor progression1

Protein4

Abnormal4

Transplantation6

Methods1

Humans2

Vivo1

Diseases1

Nucleus2

Lineages2

Barrier2

Surface6

Intercellular1

Affects1

Basal cell carc2