AnatomyOrganismsDiseasesChemicals and DrugsAnalytical, Diagnostic and Therapeutic Techniques and EquipmentPhenomena and ProcessesDisciplines and OccupationsInformation Science
Endothelial CellsUmbilical VeinsCells, CulturedHuman Umbilical Vein Endothelial CellsEndotheliumEndothelium, VascularNeovascularization, PhysiologicCattleCell AdhesionAortaVascular Endothelial Growth Factor AEndothelium, CornealCapillariesCell MovementNeovascularization, PathologicAntigens, CD31Vascular Cell Adhesion Molecule-1MicrocirculationIntercellular Adhesion Molecule-1E-SelectinSignal TransductionRNA, MessengerNitric Oxide Synthase Type IIIVascular Endothelial Growth Factor Receptor-2Cell Adhesion MoleculesNitric OxideEndothelial Growth FactorsBlood VesselsMicrovesselsCell LineVascular Endothelial Growth FactorsCapillary PermeabilityCell DivisionAngiogenesis InhibitorsLymphokinesTumor Necrosis Factor-alphaStress, MechanicalApoptosisCell ProliferationGene Expression Regulationvon Willebrand FactorReceptor, TIE-2ImmunohistochemistryPulmonary ArteryDose-Response Relationship, DrugPhosphorylationUp-RegulationBlotting, WesternFibroblast Growth Factor 2Time FactorsCoculture TechniquesMice, Inbred C57BLReceptors, Vascular Endothelial Growth FactorRetinal VesselsThrombinCell SurvivalEpoprostenolReverse Transcriptase Polymerase Chain ReactionSwineEnzyme ActivationEnzyme InhibitorsNitric Oxide SynthaseCell CommunicationCulture Media, ConditionedBlood-Brain BarrierAngiopoietin-1Receptors, Cell SurfaceAntigens, CDRNA, Small InterferingGene ExpressionCorneal Endothelial Cell LossTransfectionPericytesEndothelium, LymphaticInterleukin-8Receptors, Growth FactorNF-kappa BMuscle, Smooth, VascularRecombinant ProteinsMonocytesMolecular Sequence DataLeukocytesThromboplastinUmbilical CordP-SelectinCaveolin 1Angiogenesis Inducing AgentsLamininFlow CytometryNeutrophilsReactive Oxygen SpeciesCoronary VesselsAngiopoietin-2Mice, KnockoutMembrane ProteinsInflammationFluorescent Antibody TechniqueInterleukin-1ThrombomodulinWeibel-Palade Bodies
Vascular endothelialDysfunctionProliferationMicrovascularAorticAngiogenesisInflammationMethodsProgenitor cellMaintain endothelialAdhesionAbstractBiologyEndotheliumGlucoseHumanDifferentiationCytokinesMarkersTissueProgenitorsPathwayLymphaticLipoproteinMigrateExtracellular matrixActivationTissuesHyperpermeabilityMediatorsEpithelialMetabolicProteinVEGFFunctionalDiabetesCornealPhenotypesMonolayerMigrationPathwaysDonorHighly specializedGraftRegulationIncidenceTumorInnate immunityCytotoxicPhysiologyAltersSignificantlyGenesVivoProteinsBlood vesselNitricInflammatoryInducesStemEPCsStudy
Vascular endothelial15
  • We determine a comprehensive map of lineage-specific lncRNAs in human dermal lymphatic and blood vascular endothelial cells (LECs and BECs), combining RNA-Seq and CAGE-Seq. (nature.com)
  • Activation of the VEGFA/VEGFR2 signaling and expression of blood vascular endothelial cell (BEC) markers, such as NRP1 and EphrinB2, further differentiate these precursor cells into BECs, which then form the hierarchical network of blood vessels 4 . (nature.com)
  • Vascular endothelial cell (EC)-derived factors play an important role in endothelial-cardiomyocyte crosstalk and could save cardiomyocytes (CMs) from injury. (mdpi.com)
  • ECs were treated with oxLDL under HG conditions in the presence or absence of ROS scavengers mitoTEMPO and NAC, p38 inhibitor SB203580, FOXO1 inhibitor AS1842856 or transfected with the TXNIP siRNA, before protein expression levels of intercellular adhesion molecule 1 (ICAM‑1), vascular cell adhesion molecule‑1 (VCAM‑1), phosphorylated vascular endothelial‑cadherin (VE‑cadhedrin), VE‑cadherin and zonula occludens‑1 (ZO‑1) were measured by western blotting. (spandidos-publications.com)
  • A number of studies have previously suggested that vascular endothelial dysfunction caused by increased junctional permeability is a key event in the development and progression of atherosclerosis and atherosclerotic cardiovascular diseases ( 3 , 4 ). (spandidos-publications.com)
  • Treatment with 30 mM glucose also increased the expression of vascular endothelial growth factor (VEGF) mRNA and protein, and the expression levels of VEGF mRNA and protein were suppressed by kaempferol (10 and 30 µM). (spandidos-publications.com)
  • Among the angiogenic mediators, vascular endothelial growth factor (VEGF) is considered to be the main stimulating factor, which acts via promoting the migration and proliferation of vascular endothelial cells, and thereby stimulates the generation of new blood vessels ( 6 ). (spandidos-publications.com)
  • Qin R , Zhu H , Wang F , Song M , Lin P , Xing Y , Zhang W , Zhong M , Wang Z , . Platelet activation in diabetic mice models: the role of vascular endothelial cell-derived protein disulfide isomerase-mediated GP IIb/IIIa receptor activation. (aging-us.com)
  • Vascular endothelial cells, as a monolayer in direct contact with the flowing blood, bear the most of the wall shear stresses and have important homeostatic functions in response to stress [16] . (plos.org)
  • In this study, we explore the role of HMGB1 in advanced glycation end products (AGEs)-induced vascular endothelial growth factor A (VEGF-A) production in rat retinal ganglion cell line 5 (RGC-5) cells. (molvis.org)
  • The expression of vascular endothelial growth factor, vascular endothelial growth factor-2 and hypoxia-inducible factor-1 were detected by Western blot. (ijpsonline.com)
  • Sodium tanshinone IIA sulfonate can inhibit hypoxia induced retinal neovascularization in a dose-dependent manner, and its mechanism may be related to the regulation of vascular endothelial growth factor pathway related proteins by sodium tanshinone IIA sulfonate. (ijpsonline.com)
  • To measure intravitreal low-density lipoprotein receptor-related protein 6 (LRP6) and vascular endothelial growth factor (VEGF) levels in the eyes of patients with proliferative diabetic retinopathy (PDR) and to observe their correlation with PDR activity. (molvis.org)
  • The requirement for tumor angiogenesis has been therapeutically exploited in the clinical setting mainly by means of inhibition of the vascular endothelial growth factor family of ligands and receptors. (lu.se)
  • Epithelioid angiosarcoma is a rare malignant neoplasm found in vascular endothelial cells. (bvsalud.org)
Dysfunction17
  • Recently, endothelial dysfunction (ED) has been demonstrated in these patients, but the mechanisms remain elusive. (mdpi.com)
  • Endothelial dysfunction is a critical initiating factor contributing to cardiovascular diseases, involving the gut microbiome-derived metabolite trimethylamine N-oxide (TMAO). (nature.com)
  • This study aims to clarify the time-dependent molecular pathways by which TMAO mediates endothelial dysfunction through transcriptomics and metabolomics analyses in human microvascular endothelial cells (HMEC-1). (nature.com)
  • The discrepancies between studies on TMAO-induced endothelial dysfunction may be attributed to different cell types and animal models used, including different metabolic backgrounds, as well as to distinct TMAO treatment concentrations and durations. (nature.com)
  • Endothelial dysfunction during diabetes has been previously reported to be at least in part attributed to increased oxidized low‑density lipoprotein (oxLDL) levels mediated by high glucose (HG) levels. (spandidos-publications.com)
  • These results suggest that rosmarinic acid can exert a protective effect against oxLDL‑mediated endothelial dysfunction under HG conditions by reducing the interaction between monocytes and ECs in addition to preventing monocyte diapedesis. (spandidos-publications.com)
  • Chronic metabolic disorders, such as hyperglycemia and dyslipidemia, promote endothelial dysfunction and eventually leakage, resulting in increased monocyte diapedesis ( 5 - 7 ). (spandidos-publications.com)
  • Endothelial dysfunction during diabetes is closely associated with increased oxidized low-density lipoprotein (oxLDL) levels mediated by high glucose (HG) conditions ( 8 ). (spandidos-publications.com)
  • When the regulatory balance is adversely affected, dysfunction of endothelial cells may occur. (spandidos-publications.com)
  • Systemic autoimmune diseases, such as rheumatoid arthritis (RA), exhibit accelerated atherosclerosis (AS) [ 1 - 4 ] as a consequence of endothelial dysfunction, leading to higher incidence of cardiovascular (CV) disease (at least 2-fold enhanced CV risk) and premature and higher mortality [ 5 , 6 ]. (hindawi.com)
  • SAA was shown to exhibit causal properties in AS, as a consequence of endothelial dysfunction (elevating tissue factor, as well as a variety of cytokines/chemokines) and early lesions (biglycan synthesis) [ 15 ] to plaque destabilization by inducing matrix metalloproteinases [ 16 ]. (hindawi.com)
  • The aim of this study was to assess whether activation of nrf2 by sulforaphane in human microvascular endothelial cells prevents metabolic dysfunction in hyperglycemia. (diabetesjournals.org)
  • CONCLUSIONS- We conclude that activation of nrf2 may prevent biochemical dysfunction and related functional responses of endothelial cells induced by hyperglycemia in which increased expression of transketolase has a pivotal role. (diabetesjournals.org)
  • Janowska J, Chudek J, Olszanecka-Glinianowicz M, Semik-Grabarczyk E, Zahorska-Markiewicz B. Interdependencies among Selected Pro-Inflammatory Markers of Endothelial Dysfunction, C-Peptide, Anti-Inflammatory Interleukin-10 and Glucose Metabolism Disturbance in Obese Women. (medsci.org)
  • We have studied the relationship between clinical and biochemical parameters and C-peptide and anti-inflammatory IL-10, as well as selected markers of inflammation and endothelial dysfunction such as: CCL2, CRP, sICAM-1, sVCAM-1 and E-selectin in obese women with various degree of glucose metabolism disturbance. (medsci.org)
  • Disturbed lipid and carbohydrate metabolism are manifested by enhanced inflammation and endothelial dysfunction in patients with simply obesity. (medsci.org)
  • However, the biomarkers sICAM-1 and sE-selectin (as mechanism of this immune dysregulation markers of endothelial dysfunction) in dia- is still unclear. (who.int)
Proliferation6
  • In addition, at 48H, BPs linked to inflammatory phenotypes were activated (confirming KEGG results), while BPs linked to extracellular matrix (ECM) structural organisation, endothelial cell proliferation, and collagen metabolism were repressed. (nature.com)
  • In addition, HREC proliferation, migration and cell sprouting were determined using Cell Counting kit‑8, wound scratch and tube formation assays, respectively. (spandidos-publications.com)
  • Low PDCD4 level was associated with reduced proliferation but not apoptosis or phosphorylation of endothelial nitric oxide synthase caused by pulsatile shear stress to help maintain the homeostasis of endothelial cells. (plos.org)
  • Ample evidence shows that pro-atherosclerotic disturbed flow induces sustained activation of atherogenic genes in endothelial cells to promote their oxidation, inflammation, cell cycle progression and proliferation, whereas pulsatile shear stress tends to maintain endothelial cells in a quiescent and less proliferative state with a low level of oxidation and inflammation [16] , [17] . (plos.org)
  • Their results showed that delaying transplantation increased cell proliferation, without increasing apoptosis, consequently leading to increased graft size in the delayed condition. (jneurosci.org)
  • Epithelioid angiosarcoma (EA) is a rare variant of angiosarcoma, which is characterized by a high-grade malignant neoplasm, which consists of an atypical, multilayered, or solid endothelial proliferation and vasoformative architecture, presenting necrosis and an area of hemorrhage 1-5 . (bvsalud.org)
Microvascular3
  • RESEARCH DESIGN AND METHODS- Human microvascular HMEC-1 endothelial cells were incubated in low and high glucose concentrations (5 and 30 mmol/l, respectively), and activation of nrf2 was assessed by nuclear translocation. (diabetesjournals.org)
  • Low SDF-1 expression may contribute to the dysfunctional mobilization of bone marrow Lin - /VEGF-R2 + endothelial progenitor cells, which may contribute to microvascular injury in early diabetes. (biomedcentral.com)
  • We have used human microvascular endothelial cells (HMEC) to study the combined effects of a model air pollutant, diesel exhaust particles (DEP), and oxidized 1-palmitoyl-2-arachidonyl- sn -glycero-3-phosphorylcholine (ox-PAPC) on genome-wide gene expression. (biomedcentral.com)
Aortic5
  • En face co-immunostaining of the mouse aortic arch revealed a low level of PDCD4 in endothelial cells undergoing pulsatile shear stress. (plos.org)
  • In this study, we utilize atomic force microscopy (AFM) under isotonic and hypo-osmotic conditions to measure the mechanical properties of human aortic endothelial membranes. (aiche.org)
  • The team also exposed commercially-available human aortic endothelial cells to the flavorings. (sciencedaily.com)
  • The system is based on endothelial cells (as an example, porcine aortic endothelial cells, PAEC) which are previously expanded in vitro in conventional flatbed culture systems. (forschung3r.ch)
  • Porcine aortic endothelial cells (PAEC, used here as an example of endothelial cells in general) are expanded in conventional flatbed cell culture and then grown to confluence on microcarriers. (forschung3r.ch)
Angiogenesis6
  • A study has demonstrated that activation of the peroxisome proliferator-activated receptor g coactivator 1-α (PGC-1α)/ERRα pathway increases VEGF expression and angiogenesis in endothelial cells, which suggests that ERRα may be involved in the pathogenesis of DR ( 10 ). (spandidos-publications.com)
  • These cells can be differentiated into mature endothelial cells, which are involved in processes of angiogenesis and vessel regeneration. (intechopen.com)
  • This chapter provides an overview of the key role of hEPC in promoting angiogenesis and their potential use for cell therapy. (intechopen.com)
  • An increasing number of studies have demonstrated that EPCs are of great value in regenerative medicine and clinical applications, which can not only directly participate in angiogenesis but also participate in vascularization and endothelial repair by differentiating into endothelial cells [ 7 ]. (biomedcentral.com)
  • The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell-cell adhesion, angiogenesis process, and. (lu.se)
  • The functionality of the matured microvasculature networks was demonstrated through the enhancement of cell-cell adhesion, angiogenesis process, and perfusion tests with microparticles, FITC-dextran, and whole mouse blood. (lu.se)
Inflammation2
Methods2
  • Forty-three (43) pattern of group A streptococci in our strains of group A streptococci isolated country the present study was conducted from the patients were serotyped by stan- to determine the presence of group A strep- dard methods [ 13 ] at the Institut Für Ex- tococci in acute pharyngitis cases and in perimentelle Mikrobiologie, Jena, Germany. (who.int)
  • Her team is developing innovative culture methods for avian primary epithelial and endothelial cells to study the understanding of the host species-specificity of HPAIVs genesis. (erasmusmc.nl)
Progenitor cell2
  • The aim of this study was to identify early diabetes-related changes in the expression of genes that have been reported to be closely involved in endothelial progenitor cell migration and function. (biomedcentral.com)
  • Based on review of current literature, this study examined the expression level of 35 genes that are known to be involved in endothelial progenitor cell migration and function in magnetically sorted Lin - /VEGF-R2 + endothelial progenitor cells obtained from the bone marrow of Akita mice in the early stages of diabetes (18 weeks) using RT-PCR and Western blotting. (biomedcentral.com)
Maintain endothelial1
  • However, the routes by which macromolecules cross endothelium, the hemodynamic stresses that maintain endothelial physiology or trigger arterial disease, and the dependence of transendothelial transport on hemodynamic stresses are controversial. (imperial.ac.uk)
Adhesion8
  • Subsequently, rosmarinic acid treatment significantly reversed oxLDL‑induced overexpression of adhesion molecules and monocyte‑EC adhesion, oxLDL‑induced endothelial junction hyperpermeability and monocyte transmigration through the endothelial monolayer under HG conditions, in a dose‑dependent manner. (spandidos-publications.com)
  • Vascular cell adhesion molecule-1 and E-selectin played a minor role in 2 groups (P = 0.6). (ajtmh.org)
  • Serum from exposed mice increased endothelial cell surface vascular cell adhesion molecule-1 (VCAM-1) and intracellular adhesion molecule-1 (ICAM-1) expression and proinflammatory transcripts, and decreased ATP-stimulated nitric oxide (NO) production. (cdc.gov)
  • We investigated the activity of physiologically relevant anthocyanin metabolite signatures, derived from a previous pharmacokinetics study of 500 mg 13C5-cyanidin-3-glucoside in 8 healthy participants, on soluble vascular adhesion molecule-1 (VCAM-1) and interleukin-6 (IL-6) in human endothelial cells. (uea.ac.uk)
  • CD11a/CD18 (LFA-1) expressed on lymphocytes is known to play an important role in lymphocyte trafficking (adhesion to vascular endothelium), as well as interactions to antigen presenting cells (APC). (medscape.com)
  • Patients with leukocyte adhesion deficiency II manifest the Bombay phenotype (ie, negative for O and H blood group antigens with potential production of anti-H antibody). (medscape.com)
  • Leukocyte adhesion deficiency II may be classified as one of the congenital disorders of glycosylation (CDG), a rapidly expanding group of metabolic syndromes with a wide symptomatology and severity. (medscape.com)
  • Variants of leukocyte adhesion deficiency have also been reported, including fully expressed but nonfunctional CD18 and an E selectin that is expressed but rapidly cleaved from the cell surface (only present in soluble form). (medscape.com)
Abstract1
  • ABSTRACT A 1-year prospective study in 2 paediatric outpatient clinics in Sousse, Tunisia, aimed to determine the presence of group A streptococci in acute pharyngitis cases and carriers, and the distribution of the serotypes and biotypes. (who.int)
Biology4
  • We believe that research (in endothelial cell biology or other fields) that improves wellbeing should not at the same time contribute to societal harm and environmental degradation. (endothelial-cell.com)
  • Vascular Physiology Group, Department of Cell Biology and Physiology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico. (unboundmedicine.com)
  • Abercrombie's innovative combination of optical technology and biology underpinned the discipline of cell migration, which has since impacted many fields to explore the cellular basis of how wounds heal and how cancer cells metastasise. (edu.au)
  • The Mackenzie Cancer Research Group has been investigating the contribution of stromal cells and the tumour microenvironment to human tumour biology since the inception of our research group in 1998. (otago.ac.nz)
Endothelium2
  • Albumin-sized tracers dominantly crossed the cultured endothelium via junctions between neighbouring cells, high-density-lipoprotein-sized tracers crossed at tricelluar junctions whilst low-density-lipoprotein-sized tracers crossed through cells. (imperial.ac.uk)
  • Potential for corneal endothelium cell loss. (nih.gov)
Glucose2
  • The present study aimed to examine the effect of kaempferol on high glucose‑induced human retinal endothelial cells (HRECs) in vitro. (spandidos-publications.com)
  • found evidence and they did not require insulin therapy of endothelial cell injury in renal failure for glucose control. (who.int)
Human11
  • Use of human cells and tissues. (endothelial-cell.com)
  • To determine the effects of medications on SAA-stimulated human coronary artery endothelial cells (HCAEC). (hindawi.com)
  • The first report in 2007, on SAA-stimulated human coronary artery endothelial cells (HCAEC), exhibited a substantial and significantly higher induction of released IL-6 protein and mRNA levels as compared to HUVEC [ 17 ], as well as increased responsiveness to IL-1 β [ 18 ]. (hindawi.com)
  • Although the LSECs make up only about 3% of the total liver cell volume, their surface in a normal adult human liver is about 210 m2, or nearly the size of a tennis court. (wikipedia.org)
  • Human endothelial progenitor cells (hEPCs) are adult stem cells, located in the bone marrow and peripheral blood. (intechopen.com)
  • Cytoadherence characteristics of Plasmodium falciparum isolates in Thailand using an in vitro human lung endothelial cells model. (ajtmh.org)
  • Using an in vitro model of human lung endothelial cells, we studied different characteristics of Plasmodium falciparum isolates as potential factors for malaria severity in 2 Thai patient groups: 27 with complicated malaria and 42 with uncomplicated malaria. (ajtmh.org)
  • Application of unidirectional pulsatile shear stress to human umbilical vein endothelial cells (HUVECs) decreased PDCD4 protein but not mRNA level. (plos.org)
  • Programmed cell death 4 (PDCD4) is an important tumor suppressor in the development of various human cancers [1] and inhibits translation rather than transcription. (plos.org)
  • We are culturing human fat cells (adipocytes) and tumour cells together, to find out how adipocytes alter tumour cell phenotype and response to therapy. (otago.ac.nz)
  • Here, we propose a technique combining multi-material extrusion and ultrasound standing wave forces to create a network structure of human umbilical vein endothelial cells within a mixture of calcium alginate and decellularized extracellular matrix. (lu.se)
Differentiation6
  • In hematopoietic tissues, a group of ECs are highly specialized and constitute the vascular HSPC niche to support HSPC maturation, expansion, and differentiation. (go.jp)
  • The team visualized endothelial differentiation step-by-step from the endoderm to the HSPC niche-constituting ECs via islet1+ endothelial progenitors by live imaging-based lineage tracing. (go.jp)
  • The pattern of cellular growth has long been known, but it is the Italian developmental biologist Rita Levi-Montalcini and the American biochemist Stanley Cohen with their discovery of nerve growth factor (NGF) and epidermal growth factor (EGF), respectively, who could show how the growth and differentiation of a cell is regulated. (nobelprize.org)
  • This unique specialization of cells is termed differentiation. (nobelprize.org)
  • Overexposure to transforming growth factor b1 (TGF-ß1) induces myofibroblastic differentiation of mesenchymal stem cells (MSCs), which could be attenuated by myeloid-derived suppressor cell (MDSC) supernatant. (bvsalud.org)
  • Such information is mediated immunity, secreting the cytokine crucial to determine the optimal approach interferon-gamma (IFN) that inhibits Th2 to improve the immune response of DN cell differentiation. (who.int)
Cytokines2
  • PCS (n = 17), PCS/CFS (n = 13), and healthy controls (HC, n = 14) were screened for serum anti-endothelial cell autoantibodies (AECAs) and dysregulated cytokines. (mdpi.com)
  • Thus, it has been shown that early EPCs release cytokines that promote tissue regeneration and neovasculogenesis, whereas late EPC and endothelial colony forming cells (ECFCs) contribute to the formation of blood vessels and stimulate tube formation. (intechopen.com)
Markers3
  • The beads can be analyzed by immunofluorescence for various cell activation markers, cell integrity etc., while the plasma is used to analyze the coagulation/inflammatory status. (forschung3r.ch)
  • The autoimmune nature of diabetes and inflammatory cytokine IL-10 (as markers the major contribution of lymphocyte T- of inflammatory changes) and the soluble cells are well established. (who.int)
  • Immunohistochemical analysis that demonstrates the endothelial nature of the neoplasm is frequently positive for specific vascular markers (CD31, CD34, factor VIII), and keratin 1,2,5,6 . (bvsalud.org)
Tissue9
  • They also serve as specialized vascular niches that provide instructive signals to tissue-specific stem cells or parenchymal cells for tissue formation and repair. (go.jp)
  • in the high-dose group, the limiting membrane of retina was relatively complete and smooth, and the tissue structure of each layer could be seen. (ijpsonline.com)
  • 43 Therefore, cell sheets with enhanced thickness and mechanical properties obtained through appropriate cyclic mechanical stimulation possess great potential for bone tissue engineering applications. (researchgate.net)
  • Cell sheet-based scaffold-free technology holds promise for tissue engineering applications and has been extensively explored during the past decades. (researchgate.net)
  • Findings from this study reveal that thermo-responsive elastomer, together with mechanical conditioning, can potentially be applied to prepare high-quality cell sheets for bone tissue engineering. (researchgate.net)
  • Fetal cell/tissue transplantation has been arduously studied as a potential way to repair the injured brain. (jneurosci.org)
  • performed additional studies to compare the effect of 7 d delay and immediate transplantation on survival and integration of grafted cells into host brain tissue at 4, 7, and 14 d after transplantation. (jneurosci.org)
  • I am rather intrigued by the idea that an eye bank could crosslink the tissue without compromising the health of the endothelial cells and that this tissue might result in better visual results than standard non-crosslinked tissue. (medscape.com)
  • Endothelial cells build up the vessel wall and control the exchange between the blood and surrounding brain tissue. (lu.se)
Progenitors4
  • During development, the blood vascular system arises from endothelial cell progenitors that differentiate from mesodermal cells, mostly through the expression of the transcription factor (TF) ETV2. (nature.com)
  • They found that islet1 -expressing cells are the progenitors of the venous ECs that constitute the majority of the HSPC niche (Figure 1A). (go.jp)
  • The present results clearly demonstrate that ECs originating from the endoderm via islet1 + endothelial progenitors play a specialized role in forming a functional vascular HSPC niche (Figure 1C). (go.jp)
  • C) We have identified that islet1 + endothelial progenitors originate from endoderm and finally differentiate into posterior venous ECs in a process dependent on Bmp-Smad signaling, requiring Npas4l-Etsrp function. (go.jp)
Pathway2
  • This pathway is basally active in mesenteric artery endothelial cells, but, after exposure to intermittent hypoxia to mimic sleep apnea, nuclear factor of activated T cells c3 nuclear translocation and CSE expression are decreased, concomitant with decreased CSE-dependent vasodilation. (unboundmedicine.com)
  • We present a rate equation model for the TGF-b pathway in endothelial cells together with novel measurements. (lu.se)
Lymphatic2
  • Thereafter, a distinct subpopulation of endothelial cells lining the cardinal vein starts differentiating by expressing the TF PROX1, the master regulator of lymphatic endothelial cell (LEC) identity, via the TFs SOX18 and COUPTFII. (nature.com)
  • Other important receptors on LSECs are L-SIGN (liver/lymph node-specific ICAM-3 grabbing nonintegrin), LSECtin (liver and lymph node sinusoidal endothelial cell C-type lectin), Lyve-1 (lymphatic vessel endothelial hyaluronan receptor‐1), and LRP‐1 (low‐density lipoprotein receptor‐related protein‐1). (wikipedia.org)
Lipoprotein1
Migrate1
Extracellular matrix3
Activation11
  • In a previous study, it was reported that oxLDL treatment induced nucleotide‑binding domain and leucine‑rich repeat containing family, pyrin domain‑containing 3 inflammasome activation in endothelial cells (ECs) under HG conditions, in a manner that could be effectively reversed by rosmarinic acid. (spandidos-publications.com)
  • The effects of oxLDL‑mediated inflammasome activation on endothelial permeability under HG conditions, in addition to the effects of rosmarinic acid on these oxLDL‑mediated processes, also remain poorly understood. (spandidos-publications.com)
  • Normally differentiated LSECs prevent hepatic stellate cell activation and promote reversion to quiescence, whereas capillarized LSECs do not. (wikipedia.org)
  • The purpose of this study was to investigate the release of Endothelial microparticle (EMP)-associated protein disulfide isomerase (PDI) after endothelial cell injury induced in diabetes and the changes in platelet activation. (aging-us.com)
  • Compared with the normal diet group, the diabetic group had significantly increased plasma EMP-PDI content and accelerated platelet activation by increased GP IIb/IIIa expression. (aging-us.com)
  • The observations that IH exposure decreases NFATc3 activation and CSE-dependent vasodilation support a role for NFAT in regulating endothelial H2S production.NEW & NOTEWORTHY This study identifies the calcium-regulated transcription factor nuclear factor of activated T cells as a novel regulator of cystathionine γ-lyase (CSE). (unboundmedicine.com)
  • In this system potential "endothelial cell protectants", i.e. substances which preserve endothelial integrity and prevent activation of the endothelial cells, can be pre-incubated with the cells in the flask or co-incubated with the whole blood, and their effects assessed. (forschung3r.ch)
  • In conclusion, pulmonary exposure to MWCNT dynamically alters circulating factors, which promotes endothelial cell activation, decreased NO bioavailability, and altered functionality all directionally predicting adverse cardiovascular outcomes. (cdc.gov)
  • CONCLUSIONS: The DTPs may arise from a specific preexisting subpopulation of cancer cells with partial activation of specific drug resistance pathways. (bvsalud.org)
  • Diabetic nephropathy (DN) is a leading selectin), and soluble thrombomodulin--are cause of chronic renal failure and is a grow- providing further evidence of the relation- ing concern given the increasing incidence ship between endothelial cell activation and of type 2 diabetes. (who.int)
  • Interestingly, changes such as pericyte activation and blood-brain-barrier leakage are already observed at the early stages of the disorder, even before behavioral changes or dopaminergic cell loss can be detected, explains Gesine Paul-Visse. (lu.se)
Tissues4
  • Endothelial cells (ECs) form a semi-permeable monolayer that separates the bloodstream from underlying tissues and regulate the infiltration of blood cells and proteins through the vessel wall ( 1 , 2 ). (spandidos-publications.com)
  • hEPCs have been used for cell-based therapies due to their capacity to contribute in the re-endothelialization of injured blood vessels and neovascularization in ischemic tissues. (intechopen.com)
  • White blood cells, for example, travel through blood vessels and different tissues to fight pathogens to protect us against infection. (edu.au)
  • The results displayed that LINC00659 is upregulated in IVC tissues and isolated endothelial group cells (EPCs) of patients with LEDVT. (biomedcentral.com)
Hyperpermeability1
Mediators1
  • Th1 cells are effectors of cell- sis on these mediators. (who.int)
Epithelial2
  • Chylomicrons produced by the intestinal epithelial cells from dietary lipids have diameter up to 1000 nm which prevents them from passing through the LSEC fenestrae. (wikipedia.org)
  • Supporting proteome analyses confirmed that organic PM extracts induce a hierarchical oxidative stress response in macrophages and epithelial cells, in which the induction of electrophile-response element (EpRE) regulated genes (for example, heme oxygenase 1, catalase, and superoxide dismutase) at lower levels of oxidative stress prevented the more damaging pro-inflammatory and pro-apoptotic effects seen at higher levels of oxidative stress [ 10 ]. (biomedcentral.com)
Metabolic2
  • In addition, the number and the migratory activity of these cells are inversely correlated with risk factors such as hypertension, hypercholesterolemia, diabetes, and metabolic syndrome. (intechopen.com)
  • Cancer cells sustain their metabolic needs through nutrients and oxygen supplied by the bloodstream. (lu.se)
Protein5
  • In addition, oxLDL‑stimulated ECs under HG conditions exhibited increased phosphorylated VE‑cadherin protein levels and decreased ZO‑1 protein expression levels compared with those in untreated ECs, suggesting increased endothelial permeability. (spandidos-publications.com)
  • For this reason, they are extensively used in a wide range of applications from protein production to bacterial biofilms and endothelial cell experiments. (imperial.ac.uk)
  • High-mobility group box 1 protein (HMGB1) has been reported to be a potent proangiogenic factor induced by inflammatory stress. (molvis.org)
  • The VEGF-A protein and mRNA levels in conditioned medium of RGC-5 cells incubated with AGE-modified BSA (AGE-BSA) were examined with real-time PCR and enzyme-linked immunosorbent assay (ELISA), and BSA-treated cells were used as controls. (molvis.org)
  • Compared with the BSA controls, the RGC-5 cells incubated with AGE-BSA showed a dose- and time-dependent increase in VEGF-A mRNA and VEGF-A protein secretion in the supernatant, with the highest levels achieved at 24 h. (molvis.org)
VEGF3
  • HMGB1 is implicated in the production of VEGF-A in retinal ganglion cell line-5 (RGC-5). (molvis.org)
  • We observed a significant increase in the number of Lin - /VEGF-R2 + endothelial progenitor cells within the bone marrow in diabetic mice compared with non-diabetic mice. (biomedcentral.com)
  • Two genes, SDF-1 and SELE, were significantly differentially expressed in diabetic Lin - /VEGF-R2 + endothelial progenitor cells and six other genes, CAV1, eNOS, CLDN5, NANOG, OCLN and BDNF, showed very low levels of expression in diabetic Lin - /VEGF-R2 + progenitor cells. (biomedcentral.com)
Functional3
  • Diabetes is known to impair the number and function of endothelial progenitor cells in the circulation, causing structural and functional alterations in the micro- and macro-vasculature. (biomedcentral.com)
  • More compelling was that this particular size fraction had functional effects on endothelial cell function. (cdc.gov)
  • In the interaction with proteins, lead binds with virtually every available functional group, including sulfhydryl, amine, phosphate, and carboxyl groups, with sulfhydryl having the highest affinity. (cdc.gov)
Diabetes3
  • Normal ApoE −/− and diabetic mice were allocated to four groups (n = 15): normal diet, normal diet plus rutin, diabetic, and diabetes plus rutin. (aging-us.com)
  • Diabetes causes retinal microvasculopathy associated with pericyte cell death, microaneurysms, abnormal vascular permeability, and macular edema. (molvis.org)
  • Diabetic group ( n = 17): patients with in patients with chronic renal failure and type 2 diabetes without renal involve- under HD, and disturbances in endothelial ment (normoalbuminuria). (who.int)
Corneal4
  • The corneal thickness was similar in the two groups after three weeks. (nih.gov)
  • These results demonstrated that donor corneal endothelial cells preserved for up to one month at 34 C in 1.35% chondroitin sulfate appear to survive and function after keratoplasty as well as those preserved at 4 C for one to three days in McCarey-Kaufman medium. (nih.gov)
  • There is an increased potential for developing corneal edema in patients with low endothelial cell counts. (nih.gov)
  • We have longer-term data on corneal crosslinking for eyes with keratoconus, and there does not seem to be any great danger of endothelial decompensation over 5-10 years, but longer-term data are scarce. (medscape.com)
Phenotypes1
  • Different phenotypes and subtypes of endothelial progenitor cells (EPCs), such as early and late EPCs, have been described according to their functionality. (intechopen.com)
Monolayer2
  • Furthermore, monocyte transmigration through the endothelial monolayer was significantly increased by oxLDL treatment under HG conditions. (spandidos-publications.com)
  • Once a sufficient cell number is available, the cells are transferred to spinner flasks and mixed with collagen-coated Biosilon microcarrier beads (5 ml, equal to 800 cm 2 , Nalge Nunc International) and cultured until the cells form a confluent monolayer on the beads. (forschung3r.ch)
Migration2
  • In the 1970s, British cell biologist Michael Abercrombie first scrutinised how cells called fibroblasts move, resulting in several seminal publications on cell migration. (edu.au)
  • In addition, serum from MWCNT-exposed mice reduced cell migration in a traditional scratch assay experiment. (cdc.gov)
Pathways3
  • Numerous signaling pathways are known to be activated by cell swelling and to contribute to cell volume homeostasis. (aiche.org)
  • Cellular biomechanics and membrane tension have long been proposed to couple cell swelling to signaling pathways, however, the impact of swelling on these parameters has yet to be fully elucidated. (aiche.org)
  • Single-cell RNA sequencing identified a rare (approximately 4%) subpopulation of BL cells (DTP-like) with transcriptomic similarity to DTP cells and intermediate activity of pathways that are up-regulated in DTPs. (bvsalud.org)
Donor3
  • Of note, they found slightly better uncorrected and best-corrected visual acuity in the crosslinked donor eyes than in the noncrosslinked donor eyes at 1 year postoperatively (with most or all sutures in), but both uncorrected and best-corrected visual acuity were statistically significantly better at 3 years postoperatively (with all sutures out for ≥ 18 months) in the crosslinked donor eye group. (medscape.com)
  • The graft diameter is 7.5 mm for the recipient and 8.0 mm for the donor in both groups. (who.int)
  • 2. Endothelial cell count of the donor pre and post transplants is measured using specular microscopy (Topcon SP-3000P) at baseline (preoperative) and 1 year postoperative. (who.int)
Highly specialized1
Graft1
  • From a safety standpoint, there was no difference in endothelial cell count, graft rejection episodes, and elevated intraocular pressure between the two groups. (medscape.com)
Regulation1
  • Cell volume regulation is a fundamental property of all mammalian cells. (aiche.org)
Incidence1
  • In order of decreasing incidence, squamous cell carcinoma (SCC), basal cell carcinoma (BCC), basosquamous cell carcinoma, and melanoma account for 90% of primary malignant tumors of the hand. (medscape.com)
Tumor2
  • We recently found low level of tumor suppressor programmed cell death 4 (PDCD4) associated with reduced atherosclerotic plaque area (unpublished). (plos.org)
  • Daily treatment of tumor-bearing mice for 5 to 6 weeks with the EGFR inhibitor erlotinib markedly shrunk tumors and generated DTPs, which were analyzed by whole exome, bulk population transcriptome, and single-cell RNA sequencing. (bvsalud.org)
Innate immunity2
  • The capacity of LSECs as scavengers of blood borne waste assigns an important role of these cells in innate immunity. (wikipedia.org)
  • Until today the activity of such substances had to be tested in animal models because of complex interactions between endothelial cells, coagulation and innate immunity. (forschung3r.ch)
Cytotoxic2
  • Recurrence is mainly driven by minor subpopulations of drug-tolerant persister (DTP) cells that survive the cytotoxic drug effect. (bvsalud.org)
  • LFA-1 also plays a role of cytotoxic killing by T cells. (medscape.com)
Physiology1
  • The Nobel Prize in Physiology or Medicine is awarded for discoveries which are of fundamental importance for our understanding of the mechanisms which regulate cell and organ growth. (nobelprize.org)
Alters1
  • The overarching question the team is trying to answer is whether chaotic fluid flow in a diseased organ alters cell behaviour. (edu.au)
Significantly2
  • After implantation into the critical-sized calvarial defects of mice, the mechanically conditioned cell sheets significantly promoted new bone formation. (researchgate.net)
  • However, comparison of patients with PDR with controls revealed that the plasma levels of LRP6 were not significantly different between the two groups (p=0.636). (molvis.org)
Genes2
  • In this study, single-cell RNA-sequencing analyses show that islet1 + -derived ECs express a set of genes that reflects their unique origin even as they become heterogeneous. (go.jp)
  • Computational analysis unveiled a hitherto unknown role for ACVRL1 in relation to genes modulating the functionality of the immune cell compartment. (lu.se)
Vivo2
Proteins2
  • We propose that blood carries an as yet unknown "inflammatory potential" consisting of modified proteins or other biomolecules and reaction byproducts that affects a pathological bioactivity which can be assessed using endothelial cells as biosensors. (cdc.gov)
  • The next step would be to investigate which proteins are secreted from pericytes and endothelial cells in α-synuclein mouse models. (lu.se)
Blood vessel1
  • Flavoring chemicals widely used in e-cigarettes and other tobacco products may be toxic to the cells that line and regulate blood vessel function. (sciencedaily.com)
Nitric4
  • Researchers found all nine flavors were dangerous to cells in the laboratory at the highest levels tested and all the flavorings impaired nitric oxide production in endothelial cells in culture (outside of the body). (sciencedaily.com)
  • Researchers found that both groups of smokers had a similar deficit in nitric oxide production when stimulated by a chemical called A23187. (sciencedaily.com)
  • Nonsmokers' cells that were treated with menthol or a clove flavoring also had impaired nitric oxide production, suggesting those flavorings cause damage like that found in active smokers. (sciencedaily.com)
  • Menthol applied to the cells increased IL-6 at high concentrations and reduced nitric oxide even at low doses. (sciencedaily.com)
Inflammatory1
  • Inflammatory right pleural effusion with mesothelial cells in 2015. (scirp.org)
Induces1
Stem3
  • Researchers identify the origin of endothelial cells that constitute the vascular niche for hematopoietic stem and progenitor cells in zebrafish. (go.jp)
  • Endothelial cells (ECs) line blood vessels and can serve as specialized vascular niches for hematopoietic stem and progenitor cells (HSPCs), a special environment where HSPCs reside and self-renew. (go.jp)
  • However, since 2009 Associate Professor Currie's interests have expanded to include the contribution to tumour progression made by tumour infiltrating immune cell populations, breast cancer stem cell-like populations, and obesity-related factors. (otago.ac.nz)
EPCs2
  • At present, with the in-depth understanding of endothelial progenitor cells (EPCs) and the increase of research, it has become the focus of medical research [ 6 ]. (biomedcentral.com)
  • Therefore, EPCs may serve as potential seed cells for new approaches to biological treatment of LEDVT. (biomedcentral.com)
Study5
  • Together, our study provides several lines of evidence supporting the intriguing concept that every cell type expresses precise lncRNA signatures to control lineage-specific regulatory programs. (nature.com)
  • Therefore, the present study aimed to elucidate the mechanisms involved in oxLDL‑induced endothelial permeability and monocyte diapedesis under HG conditions, in addition to the potential effects of rosmarinic acid. (spandidos-publications.com)
  • The study detected with 90% power a difference in cell loss of approximately 10% or more. (nih.gov)
  • Also, the study gauged just the flavorings' short-term effects and captured these with cells outside the body, not inside. (sciencedaily.com)
  • A study published in Scientific Reports identifies pericytes as potential target cells for early interventions. (lu.se)