• mutant gonads
  • Further examination revealed reduction in cell proliferation and abnormal somatic cell differentiation in the nr0b1 mutant gonads at the undifferentiated and bi-potential ovary stages. (zfin.org)
  • formation
  • Previously, we have shown that gonad formation in Drosophila requires the homophilic cell-adhesion molecule Drosophila E-cadherin (DE-cadherin), and also Fear of Intimacy (FOI), which is required for stable accumulation of DE-cadherin protein in the gonad. (biologists.org)
  • Formation of this organ requires that germ cells and somatic gonadal precursors (SGPs), which are initially specified far apart from each other, undergo a complex morphogenetic program to form a properly patterned embryonic gonad. (biologists.org)
  • Although many genes involved in germ cell migration and SGP specification have been characterized, only three downstream genes have been identified that specifically coordinate the morphogenetic movements of gonad formation. (biologists.org)
  • suggesting that foi may act upstream of DE-cadherin to regulate gonad formation. (biologists.org)
  • development
  • This gene is considered the sex-determining gene in chicken, because among genes that govern sexual development, Dmrt1 resides on the sex chromosomes, acts in a sex-specific pattern in the gonad earliest among those genes, and sex is reversed when Dmrt1 expression is suppressed [ 12 ]. (royalsocietypublishing.org)
  • Here, we present an in vivo structure-function analysis of FOI that strongly indicates that zinc transport activity of FOI is essential for gonad development. (biologists.org)
  • expression
  • Mutant forms of FOI that are defective for zinc transport also fail to rescue morphogenesis and DE-cadherin expression in the gonad. (biologists.org)
  • We further show that expression of DE-cadherin in the gonad is regulated post-transcriptionally and that foi affects this post-transcriptional control. (biologists.org)
  • Expression of DE-cadherin from a ubiquitous ( tubulin ) promoter still results in gonad-specific accumulation of DE-cadherin, which is strongly reduced in foi mutants. (biologists.org)
  • The nr0b1 mutation did not increase Caspase-3 labeling nor tp53 expression in the developing gonads. (zfin.org)