• Extraction procedures are optimized to precipitate and remove proteins and nucleic acids while maximizing solubilization of GSLs along with other lipids. (springer.com)
  • Provided herein are anti-angiogenic comprising the N-terminal end of endostatin, nucleic acids encoding the same, pharmaceutical preparations comprising an effective amount of the peptide and nucleic acids and use of the pharmaceuticals in treating or preventing diseases or conditions associated with undesirable angiogenesis. (justia.com)
  • Like amino acids and nucleic acids, sugars are the major contributors in nature many natural products contain oligosaccharides that are important for their biological and biochemical activity and carbohydrates have major roles in a wider range of biological processes including signal transduction mechanisms and immune responses. (biochemistryconferences.com)
  • active site - A region in proteins and nucleic acids (that participate in chemical reactions), where reacting molecules (substrates) bind and make specific contacts necessary for chemical catalysis. (rcsb.org)
  • methylation at certain residues of histones can regulate gene expression [ 4 ], and glycosylation is responsible for targeting substrates and changing protein half-life [ 2 ]. (hindawi.com)
  • It is characterized by hydrogen bonds between the carbonyl group (-C=O) of one amino acid and the amino (N-H) group of the amino acid 4 residues below it along the helix. (rcsb.org)
  • The human cellular prion protein (PrP C ) is a glycosylphosphatidylinositol (GPI) anchored membrane glycoprotein with two N-glycosylation sites at residues 181 and 197. (mdpi.com)
  • N -linked glycosylation occurs when glycans are attached to asparagine residues on the protein. (neb.com)
  • O -linked glycans are most commonly attached to serine or threonine residues through the N -Acetylgalactosamine residue. (neb.com)
  • N-linked glycosylation occurs through the asparagine residues of the protein, while O-linked glycosylation occurs through serine or threonine. (neb.com)
  • Finally, these new peptides reveal a carbohydrate moiety, indicating a glycosylation in the sequence. (springer.com)
  • Descriptions of specific amino acid, carbohydrate, or nucleotide sequences which have appeared in the published literature and/or are deposited in and maintained by databanks such as GENBANK, European Molecular Biology Laboratory (EMBL), National Biomedical Research Foundation (NBRF), or other sequence repositories. (lookformedical.com)
  • In several examples, the α4 integrin antagonist is a monoclonal antibody that specifically binds to a α4, β1 or β7 integrin subunit or a cyclic hexapeptide with the amino acid sequence of CWLDVC. (justia.com)
  • Comparison of the Cry1Ia1 amino acid sequence to known amino acid sequences revealed no significant homology to known toxins or known allergens. (ashs.org)
  • Another alias for this gene is TPR repeat protein 16, but this is not commonly used. (wikipedia.org)
  • The molecular weight is 98.3 kdal and the isoelectric point is 9.15 making TTC16 a basic protein. (wikipedia.org)
  • In total, the TTC16 protein is 873 amino acids in length. (wikipedia.org)
  • There is only one known case of TTC16 interaction, involving polymerase basic protein 2 (pb2). (wikipedia.org)
  • Whereas the trisaccharidic head group of Gb 3 defines the specificity of Shiga toxin binding, the lipophilic part composed of sphingosine and different fatty acids is suggested to determine its localization within membranes impacting membrane organisation and protein binding eventually leading to protein internalisation. (springer.com)
  • Either at genomic or at proteomic level, mutations have significant impact on normal gene or protein function, and human diseases could be associated with mutations like nonsynonymous single-nucleotide variations (nsSNVs) on amino acids. (hindawi.com)
  • 13 , 14 However, the amino acid perfectly maintains the stability of the mutual structural conformation of the virus S-protein and the ACE2 receptor in a holistic manner. (medsci.org)
  • 3-10 helix - A type of protein secondary structural element that is more tightly coiled than the alpha helix (3 amino acids per turn with 10 atoms in the ring completed by each intra-helical hydrogen bond). (rcsb.org)
  • alignment - A comparison of two or more gene or protein sequences in order to determine their degree of similarity in amino acid or bases, respectively. (rcsb.org)
  • Differences in pathogenicity and virulence in mink between these strains could be related to clear amino acid differences in the non structural 1 (NS1) protein. (biomedcentral.com)
  • PMM2-CDG is typically classified as a disorder of N-linked protein glycosylation . (cdghub.com)
  • Glycosylation defines the adhesive properties of glycoconjugates and it is largely through glycan-protein interactions that cell- cell and cell-pathogen contacts occur, a fact that highlights the importance of glycobiology. (neb.com)
  • Recombinant proteins are generally produced in mammalian host cells to ensure that protein therapeutics are correctly folded and have the right posttranslational modifications, such as glycosylation, to ensure they are tolerated by patients. (bioradiations.com)
  • 13 , 14 Despite the presence of amino acid mutations in its receptor binding domain, multiple key amino acids are changed. (medsci.org)
  • Using mass spectrometers, they monitor enzyme reactions, confirm amino acid sequences, and identify large proteins from databases that include samples derived from proteolytic fragments. (chromatographyonline.com)
  • somewhat similar sequences of about 110 amino acids each. (wikipedia.org)
  • Plasmid production is commonly achieved through recombinant Escherichia coli fed batch fermentation, during which the appropriate genetic sequences are amplified, harvested, purified, and tested for safety. (cellculturedish.com)
  • For the first time, the researchers were able to characterize new bacteria with important functional properties: For example Flintibacter butyricum produces the short-chain fatty acid butyrate from both sugars and proteins - a rare property in the realm of intestinal bacteria. (vetscite.org)
  • Other commonly used endoglycosidases such as Endoglycosidase H ( NEB #P0702 ) are not suitable for general deglycosylation of N -linked sugars, because it only deglycosylates glycoproteins containing primarily high mannose N -linked structures, as well as leaving one N -acetylglucosamine residue attached to the asparagine. (neb.com)
  • Phosphorylation of animal toxins has not been described in the literature so far, in contrast to glycosylation for which few articles were published [ 20 ]. (springer.com)
  • Second, the new peptides do not contain disulfide bonds, a post-translational modification commonly encountered in animal toxins. (springer.com)
  • Based on these findings, the disclosure features anti-angiogenic peptides comprising at least about 12 amino acids of SEQ ID Nos. (justia.com)
  • There are 20 different alpha amino acids commonly found in nature that can covalently link with each other to form short peptides or longer proteins. (rcsb.org)
  • With the development of high throughput technologies such as tandem mass spectrometry (MS/MS) and next generation sequencing, more and more nonsynonymous single-nucleotide variations (nsSNVs) that cause variation of amino acids have been identified, some of which result in the damage of PTMs. (hindawi.com)
  • Sialic acid is a generic term for the N- or O-substituted derivatives of neuraminic acid, a monosaccharide with a nine-carbon backbone and are found widely distributed in animal tissues and to a lesser extent in some other organisms, ranging from fungi,plants,yeasts and bacteria, mostly in glycoproteins and gangliosides. (biochemistryconferences.com)
  • For example, the backbone carbon atom linked to the carbonyl group in an alpha amino acid, to which the side chain is attached. (rcsb.org)
  • amino acid - A building block of proteins is an alpha amino acid which contains a basic amino group, an acidic carboxyl group, and a hydrogen or organic side chain attached to the central carbon atom. (rcsb.org)
  • Beta-crystallins exist as oligomers formed from acidic (BETA-CRYSTALLIN A CHAIN) and basic (BETA-CRYSTALLIN B CHAIN) subunits. (lookformedical.com)
  • Based on the ganglio GSL series, gangliosides are synthesized, where sialic acids are linked to the glycan structure to produce negatively charged GSLs. (springer.com)
  • After separation by TLC, 10 -12 -10 -9 mol quantities of GSL can be chemically detected with orcinol, a reagent for hexoses and with resorcinol-HCl, a reagent for sialic acid. (springer.com)
  • cGMP plasmid DNA is an essential starting material for viral vector production but current manufacturing strategies based on methods adapted from the basic research laboratory lack the efficiency and consistency to meet yield requirements. (cellculturedish.com)
  • Causes of obstruction include kidney stones, tumours, or blood clots, commonly in the bladder outlet. (randox.com)
  • Phosphomannomutase 2 congenital disorder of glycosylation (PMM2-CDG) is a rare autosomal recessive genetic disorder that arises from defects in the PMM2 (phosphomannomutase 2) gene 1,2 . (cdghub.com)
  • To obtain fluorescent Gb 3 derivatives, either fatty acid labelled Gb 3 molecules or head group labelled ones were synthesized. (springer.com)
  • Though many methods are used in the process of obtaining pure proteins, chromatography is commonly used to separate complex biological mixtures and isolate proteins of interest prior to downstream characterization. (bioradiations.com)
  • 2 Typical fermentation media is composed of undefined components, like peptone or hydrolysate mixtures with a wide range of amino acid concentrations (Figure 1) resulting in yield, quality and purity inconsistencies in manufacturing. (cellculturedish.com)
  • Special emphasis is put on the preparation of Gb 3 molecules differing only in their fatty acid part (saturated, unsaturated, α-hydroxylated and both, unsaturated and α-hydroxylated). (springer.com)
  • The many transcription factors of TTC16 are commonly found in cells of the immune system, including several types of white blood cells and red blood cells, the respiratory system, and the endocrine system. (wikipedia.org)
  • The transporter of negative transcription results in throughput amino in economic aspects develops the I of intracellular acids. (erik-mill.de)
  • In addition to providing complete and holistic patient care, He is involved in basic, translational, and clinical research on myelination in Multiple Sclerosis. (gr.jp)
  • The National Center for Gender Physiology is NASA and NIH funded Center was the first-of-its-kind in the country with a mission to serve as a catalyst for the development of multidisciplinary, integrated collaborations in basic and clinical research in the arena of gender-based biology and medicine. (arizona.edu)
  • Her research group uses interdisciplinary approaches in chemistry, engineering, and biophysics to study complex biological systems and to develop new tools for basic science and biomedical applications. (stanford.edu)
  • alanine (Ala, A) - Alpha amino acid with a non-polar side chain. (rcsb.org)
  • The results again demonstrate that the fatty acid of Gb 3 plays a pivotal role for the overall membrane organisation. (springer.com)
  • It is the most frequent congenital disorder of glycosylation (CDG) with more than 1000 patients reported in the medical literature to date. (cdghub.com)
  • These cookies ensure basic functionalities and security features of the website, anonymously. (sussex-research.com)
  • Chemistry, sample preparation, and data handling are considered, as well as the definition of some terms commonly in use in the most prevalent forms of MS practice today. (chromatographyonline.com)
  • These questions, which emerged from the colloquium "Darwins' reversals: What we now know about Feralization and Crop Wild Relatives" at the BOTANY 2021 conference, fall into seven categories that span both basic and applied research: (1) definitions and drivers of ferality, (2) genetic architecture and pathway, (3) evolutionary history and biogeography, (4) agronomy and breeding, (5) fundamental and applied ecology, (6) collecting and conservation, and (7) taxonomy and best practices. (weizmann.ac.il)
  • It is a small but rapidly growing field, with relevance to biomedicine, biotechnology and basic research. (neb.com)
  • Endostatin, a 183 amino acid proteolytic cleavage fragment corresponding to the C-terminus of collagen 18, has been the subject of investigation by a number of laboratories because of its anti-tumor activity with no toxic side effects (O'Reilly et al. (justia.com)
  • In entomology the cell layer of the body wall is commonly called the "hypodermis", but the term epidermis is preferable inasmuch as the integumental epithelium is the homologue of the ectodermal layer of the skin so designated in vertebrate anatomy, though either term is illogical when applied to invertebrates having no accompanying "dermis" (Snodgrass, 1935). (collembola.org)
  • Our main goals are to develop new technologies for basic science and biomedical applications. (stanford.edu)