• Objective: The indiscriminate use of antibiotics has led to the creation of resistant microorganisms, with the aim of this study was to compare in vitro the degree of effectiveness of beta-lactam antibiotics and glycopeptide in the inhibition of Staphylococcus aureus by comparing its effectiveness in surgical wound infection. (bvsalud.org)
  • Glycopeptide antibiotics (GPAs) are effective against serious Gram-positive bacterial pathogens including methicillin-resistant Staphylococcus aureus. (mcmaster.ca)
  • Vancomycin is in a class of medications called glycopeptide antibiotics. (medlineplus.gov)
  • For glycopeptide characterization, mass spectrometry has arisen as the go-to methodology. (news-medical.net)
  • Yang's group developed a fine-tuned MS/MS acquisition method and pGlyco 2.0 search engine to perform confident intact glycopeptide characterization ( 13 ). (frontiersin.org)
  • The synthesis of glycopeptides provides biological probes for researchers to elucidate glycan function in nature and products that have useful therapeutic and biotechnological applications. (wikipedia.org)
  • Several methods have been reported in the literature for the synthesis of glycopeptides. (wikipedia.org)
  • Within solid phase peptide synthesis (SPPS) there exist two strategies for the synthesis of glycopeptides, linear and convergent assembly. (wikipedia.org)
  • Linear assembly remains a popular strategy for the synthesis of glycopeptides with many examples in the literature. (wikipedia.org)
  • Another strategy to produce glycopeptide libraries is using Glyco-SPOT synthesis technique. (wikipedia.org)
  • The glycopeptides are produced by spotting FMOC protected amino acids allowing the synthesis to be performed at microgram (nanomole) scale using very small amounts of glycoamino acids. (wikipedia.org)
  • Carbohydrate chemistry Glycopeptide antibiotic Glycosylation Peptide synthesis Talbot P. (wikipedia.org)
  • A solid-phase Mitsunobu reaction between a resin-bound 1-thiosugar and an N-Fmoc protected amino alcohol was successfully employed for thio-linked glycopeptide synthesis. (brad.ac.uk)
  • Koksch B, Sewald N, Jakubke H-D, Burger K. Synthesis and Incorporation of alpha-Trifluoromethyl Substituted Amino Acids into Peptides and Glycopeptides. (uni-bielefeld.de)
  • Synthesis and Incorporation of alpha-Trifluoromethyl Substituted Amino Acids into Peptides and Glycopeptides" in Biomedical frontiers of fluorine chemistry , Ojima, I., McCarthy, J. R., and Welch, J. T. eds. (uni-bielefeld.de)
  • In this context, this work describes the synthesis of the hypoglycosylated alpha-DG mimetic glycopeptide NHAc-Gly-Pro-Thr-Val-Thr[alphaMan]-Ile-Arg-Gly-BSA (1) as a potential tool for the development of novel antibodies applicable to dystroglycanopathies diagnosis. (cnr.it)
  • Glycopeptides are peptides that contain carbohydrate moieties (glycans) covalently attached to the side chains of the amino acid residues that constitute the peptide. (wikipedia.org)
  • We have identified a novel class of peptides bound to MHC class II: mannose 6- phosphate (Man-6-P) containing glycopeptides from lysosomal enzymes. (ox.ac.uk)
  • Lectin purified human class I MHC-derived peptides: evidence for presentation of glycopeptides in vivo. (ox.ac.uk)
  • The term glycopeptide refers to a group of antimicrobial agents that includes vancomycin, teicoplanin, and several other antimicrobial agents currently in U. S. clinical trials. (cdc.gov)
  • Why is the term "glycopeptide" used, instead of vancomycin, to describe the decreased susceptibility of these CoNS isolates? (cdc.gov)
  • Because of its broader scope, the term glycopeptide is used instead of vancomycin to describe these isolates. (cdc.gov)
  • Vancomycin is a complex glycopeptide that binds to precursors of the peptidoglycan layer in bacterial cell walls. (msdvetmanual.com)
  • Vancomycin : It is a glycopeptide antibiotic discovered in 1956 as a penicillin substitute which has assumed special significance due to efficacy against MRSA, Strep. (pharmacy180.com)
  • For example, administration of vancomycin, a glycopeptide antibiotic, to neonatal mice exacerbated antigen-induced lung inflammation that was assessed by enumerating the number of bronchoalveolar lavage (BAL) eosinophils. (cdc.gov)
  • You might want to verify that your instrument(s) are really doing what they say they're doing if you're thinking about intact glycopeptide work! (blogspot.com)
  • 1,2 discussed a new glycopeptide instant expertise method which makes use of multiple collision energies to produce glycan fragments and peptide fragments inside the same MS/MS spectrum. (news-medical.net)
  • Until recently, classification of N-glycopeptide spectra in ProteinScape (detection of glycopeptide spectra and calculation of glycan and peptide moiety mass) has centered on glycan fragment distances and oxonium ion detection when using typical CID conditions on ion trap or qTof instruments (see application note LCMS-66)4. (news-medical.net)
  • 1,2 employed synthetic, and therefore well-defined, N-glycopeptides for the optimization of CID energy parameters, so as to attain the greatest possible quantities of data on both the glycan and peptide moiety within just one tandem MS experiment. (news-medical.net)
  • Consequently, glycopeptides consisting of the same peptide bearing different glycan structures will all possess very similar retention times and co-elute. (covid19-msc.org)
  • Native chemical ligation (NCL) is a convergent synthetic strategy based on the linear coupling of glycopeptide fragments. (wikipedia.org)
  • Synthetic glycopeptide 7 AA fragment (Tyr1-Thr7) with Glcβ at Thr7. (sussex-research.com)
  • Previously, using synthetic glycopeptides carrying a natural cytosolic type of monosaccharide O-beta-linked N-acetylglucosamine (GlcNAc) glycosylation of serine residues, we have shown that glycopeptides act as suitable substrates for TAP-mediated transport into the endoplasmic reticulum (ER), and that they bind efficiently to class I major histocompatibility complex (MHC) molecules and can elicit glycopeptide-specific cytotoxic T-lymphocyte (CTL) responses in mice. (ox.ac.uk)
  • Tolerance has been described for anti-staphylococcal beta-lactams but involves also glycopeptide antimicrobial agents. (biomedcentral.com)
  • Despite the fact that the classification workflow in ProteinScape has an explicit parameter to address this issue, the peptide mass of low abundant N-glycopeptides can be miscalculated at times, which can result in no or incorrect identifications. (news-medical.net)
  • In the case of N-glycopeptide fragmentation, the higher collision energies, which are required for peptide moiety fragmentation, also bring about a characteristic fragmentation pattern, which has earlier been described for MALDI fragmentation 3 . (news-medical.net)
  • The obtained results demonstrate that the Chemical properties of the glycopeptides studied are determined by the structure and length of the peptide chain, suggesting that similar products may be also generated in the early stages of the Maillard reaction in vivo . (srce.hr)
  • They handily fragment well right at the peptide bond, but these authors show what appears to be a huge difference in N-glycopeptide identification with an HCD change from 30 to 35 (!yikes! (blogspot.com)
  • Molecular modelling of glycopeptide 1 and GpalphaDG1 scFv suggested that their interaction occurs through hydrogen bonds and hydrophobic contacts involving amino acids from scFv (I51, Y33, S229, Y235, and P233) and R8 and alpha-mannose from Glycopeptide 1. (cnr.it)
  • LAUSANNE, Switzerland, June 15, 2022 - Gnubiotics Sciences, a biotech company pioneering immunomodulatory glycopeptides, today announced an agreement with the University of Pennsylvania to explore combining CAR-T therapy with Gnubiotics' GLAAD technology to enhance efficacy in solid tumors through company-sponsored pre-clinical research studies in the laboratory of Avery Posey, PhD, an assistant professor of Systems Pharmacology and Translational Therapeutics at the Perelman School of Medicine at Penn. (gnubiotics.com)
  • Here we show that the regiochemistry of the most common N-acetylneuraminic acid linkages of N-glycans can be identified in a site-specific manner from individual glycopeptides using ion mobility-mass spectrometry analysis of diagnostic fragment ions. (fu-berlin.de)
  • TACA-containing glycopeptides are appealing cytotoxic T cell (CTL)-based vaccines to prevent or treat cancer because the same sugar moieties are expressed in a variety of tumors, rendering a vaccination strategy applicable in a large population. (silverchair.com)
  • Gnubiotics presented compelling data recently at the 2022 American Society of Clinical Oncology (ASCO) annual meeting showing the effect of its glycopeptides platform in driving directed and robust T-cell immune responses against PD-1 resistant solid tumors in a colorectal cancer mouse model. (gnubiotics.com)
  • Sensitivity of S. aureus to beta-lactam and glycopeptide. (bvsalud.org)
  • Pulsed-field gel electrophoresis restriction fragment patterns of Sma I-digested genomic DNA obtained from glycopeptide-resistant Enterococcus faecium isolated at San Vicente de Paul Hospital, Bogotá, Colombia. (cdc.gov)
  • Lycium barbarum polysaccharide (LBP) is an active ingredient extracted from Lycium barbarum that inhibits neuroinflammation , and Lycium barbarum glycopeptide (LbGp) is a glycoprotein with immunological activity that was purified and isolated from LBP. (bvsalud.org)
  • It is possible to achieve glycopeptide fragmentation under standard collision induced dissociation (CID) conditions, in which the glycosidic bonds between carbohydrate bonds are favourably cleaved. (news-medical.net)
  • To find a way around this issue, deeper investigation of the fragmentation of N-glycopeptides was carried out using Bruker oTOF instruments. (news-medical.net)
  • News in Proteomics Research: Optimal Fragmentation of N- and O- Glycopeptides! (blogspot.com)
  • Specific mass spectral detection of glycopeptides from protein digests may be achieved by on-line HPLC-MS, with selected ion monitoring (SIM) for diagnostic product ions generated by cone voltage fragmentation, or by precursor ion scanning for terminal saccharide product ions, which can yield the same information more rapidly. (lincoln.ac.uk)
  • The best-characterized VH and VL domains were cloned, expressed in E. coli Shuffle T7 cells, and used to construct a single chain fragment variable that recognized the Glycopeptide 1 (GpalphaDG1 scFv). (cnr.it)
  • This quantitative glycopeptide standard was developed for use during the sialic acid or monosaccharide release and labeling process. (qa-bio.com)
  • We identified a total of 587 glycoproteins, including 1239 glycopeptides, 526 monosaccharide components, and 8326 intact glycopeptides in glycoproteomics, as well as a total of 4496 proteins identified in proteomic analysis. (frontiersin.org)
  • Product ion spectra have been obtained on many glycopeptides confirming structure assignments. (lincoln.ac.uk)
  • The glycopeptide fractions were further purified by gel-permeation chromatography and two purified glycopeptides, D2H, D2L, were obtained. (scirp.org)
  • Currently, analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) of intact glycopeptides is often the method of choice in site-specific glycoproteomic studies ( 12 ). (frontiersin.org)
  • Compound classification and ensuing glycopeptide identification is executed with ProteinScape 4.0 and the embedded search algorithm GlycoQuest. (news-medical.net)
  • It is a glycopeptide related to RISTOCETIN that inhibits bacterial cell wall assembly and is toxic to kidneys and the inner ear. (lookformedical.com)
  • All clinical isolates with decreased susceptibility to glycopeptides have been oxacillin resistant and resistant to many other commonly used therapeutic agents. (cdc.gov)
  • Like oxacillin-resistant CoNS, isolates with decreased susceptibility to glycopeptides can show heteroresistance. (cdc.gov)
  • Isolates of S. haemolyticus and S. epidermidis with decreased susceptibility to glycopeptides have been reported. (cdc.gov)
  • Not all routine susceptibility test methods can detect CoNS isolates with decreased susceptibility to glycopeptides. (cdc.gov)
  • Here we demonstrate that by using glycopeptides with high affinity for the major histocompatibility complex and glycosylated in a position corresponding to a critical T cell receptor (TcR) contact, it is possible to induce anti-TACA CTL in vivo. (silverchair.com)
  • GLAAD relies on glycopeptides as providers of antigenicity and adjuvanticity to the immune system. (gnubiotics.com)
  • Glycopeptides are the foundation of Gnubiotics' GLAAD molecules. (gnubiotics.com)
  • Accordingly, polyclonal antibodies were reactive to glycopeptide 1, which enables the application of anti-glycopeptide 1 antibodies in immune reactive assays targeting hypoglycosylated alpha-DG. (cnr.it)
  • In the current study we show that designer glycopeptides containing the Thomsen-Freidenreich (TF) antigen (β-Gal-[1→3]-α-GalNAc- O -serine) are immunogenic in vivo and generate TF-specific CTL capable of recognizing a variety of tumor cells in vitro including a MUC1-expressing tumor. (silverchair.com)
  • The sugar portion of the glycopeptides was composed primarily of glucose (Glc) and mannose (Man) with the molar ratio of 9:1. (scirp.org)
  • A mannose 6-phosphate-containing N-linked glycopeptide derived from lysosomal acid lipase is bound to MHC class II in B lymphoblastoid cell lines. (ox.ac.uk)
  • As well as the instrumental arrangement, a suitable software solution is essential to enable glycopeptide identification and cut laborious processing and de novo analysis time. (news-medical.net)
  • Together, these data provide further evidence for the natural presentation by human class I MHC of glycopeptides carrying terminal O-GlcNAc residues in vivo. (ox.ac.uk)
  • Unlike standard SPPS (which is limited to 50 amino acid residue) NCL allows the construction of large glycopeptides. (wikipedia.org)
  • Lycium barbarum glycopeptide alleviates neuroinflammation in spinal cord injury via modulating docosahexaenoic acid to inhibiting MAPKs/NF-kB and pyroptosis pathways. (bvsalud.org)
  • What CoNS species with decreased susceptibility to glycopeptides have been reported? (cdc.gov)
  • GlycoQuest (search algorithm in ProteinScape) is Bruker's proven solution and delivers dependable results with regards to glycopeptide identification. (news-medical.net)
  • We are excited about the promise of combining CAR-T with glycopeptide strategies like our GLAAD platform which will hopefully lead to the application of new modalities for patients left with few alternative treatments," stated Yemi Adesokan, Ph.D., Gnubiotics's Chief Executive Officer. (gnubiotics.com)
  • Any variety of glycopeptide sample can be employed in this workflow - low complex and high complex, with and without glycopeptide enrichment. (news-medical.net)
  • In this method, libraries of glycopeptides are produced on a cellulose surface (e.g. filter paper) which acts as the solid phase. (wikipedia.org)
  • Collision energy stepping implemented in the Glycopeptide Instant Expertise™ method. (news-medical.net)
  • Typical glycopeptide CID spectrum achieved by using the Glycopeptide Instant Expertise™ method. (news-medical.net)
  • Differentiating the structure of isobaric glycopeptides represents a major challenge for mass spectrometry-based characterisation techniques. (fu-berlin.de)
  • The crystal structure of MtfA complexed with (S)-adenosyl-L-methionine, (S)-adenosylhomocysteine, or sinefungin inhibitor, coupled with mutagenesis, identified His228 as a likely general base required for methyl transfer to the N terminus of the glycopeptide. (mcmaster.ca)
  • 2001). 'Ester-linked Glycopeptides as Tools for Studies of Biological Phenomena', Croatica Chemica Acta , 74(4), str. (srce.hr)
  • Horvat Š, Jerić I, Varga-Defterdarović L, Roščić M, Horvat J. Ester-linked Glycopeptides as Tools for Studies of Biological Phenomena. (srce.hr)
  • The glycosidic linkages in the purified glycopeptides were analysed via methylation analysis. (scirp.org)
  • However to produce larger quantities of glycopeptides traditional resin-based solid phase techniques would be better. (wikipedia.org)
  • It would suck to optimize on your Fusion 1 and then move that to another instrument and waste all those glycopeptide runs! (blogspot.com)
  • Because of these encouraging results, we applied the same antigenic strategy to TACA-containing glycopeptides because induction of a degenerate T cell response against this class of tumor antigens may be of great advantage in cancer immunotherapy ( 10 - 12 ). (silverchair.com)
  • The glycopeptides are involved in all three types of decay. (irg-wp.com)
  • Ligand amplification in a dynamic combinatorial glycopeptide library. (ox.ac.uk)
  • N-acetyl glucosamine binding protein amplifies the concentration of one member in a dynamic combinatorial glycopeptide library based on exchanging disulfides. (ox.ac.uk)