• DHAP
  • Using this shuttle system, NADH generated by cytosolic metabolisms including glycolysis is reoxidized to NAD+ reducing DHAP to G3P, and the reducing equivalent can be used for generating a proton gradient across the mitochondrial inner membrane by coupling and oxidizing G3P and reducing quinone. (wikipedia.org)
  • The enzyme first uses Asp71 to specifically abstract the pro-S hydrogen from the C-3 of DHAP to form an ene-diol(ate)-enzyme intermediate, unlike the abstraction of C-3 pro-R hydrogen in TIM by Glu165. (wikipedia.org)
  • biosynthetic
  • Cells adapted to 25°C expressed changes in the abundance of 219 unique proteins representing a broad range of categories such as translation, glycolysis, biosynthetic (anabolic) processes, NAD, cytoskeletal organization, RNA processing, oxidoreductase activity, response-to-stress and cell redox homeostasis. (biomedcentral.com)
  • fatty
  • The hydrolysis, saponification, or transesterification of these triglycerides produces glycerol as well as the fatty acid derivative: Triglycerides (1) are treated with an alcohol such as ethanol (2) with catalytic base to give ethyl esters of fatty acids (3) and glycerol (4): Typical plant sources include soybeans or palm. (wikipedia.org)
  • Fatty acids are released, between meals, from the fat depots in adipose tissue, where they are stored as triglycerides, as follows: Lipolysis, the removal of the fatty acid chains from the glycerol to which they are bound in their storage form as triglycerides (or fats), is carried out by lipases. (wikipedia.org)
  • Once freed from glycerol, the free fatty acids enter the blood, which transports them, attached to plasma albumin, throughout the body. (wikipedia.org)
  • byproduct
  • Production will increase as the EU directive 2003/30/EC are implemented, which required the replacement of 5.75% of petroleum fuels with biofuel across all member states by 2010, as glycerol is a byproduct in the production of biodiesel. (wikipedia.org)
  • form
  • The nucleophilic glutamate 165 residue of TPI deprotonates the substrate, and the electrophilic histidine 95 residue donates a proton to form the enediol intermediate. (wikipedia.org)
  • Besides the precisely placed glutamate and histidine residues to form the enediol, a ten- or eleven-amino acid chain of TPI acts as a loop to stabilize the intermediate. (wikipedia.org)
  • A second base deprotonates the hydroxyl group, leading to the collapse of the en-diol(ate) to form the 2-hydroxy 2-propenal enol intermediate along with dissocation of inorganic phosophate (-OPO3) through the cleavage of a C-O bond rather than an O-P bond. (wikipedia.org)
  • organic
  • Crude glycerol from the hydrolysis of triglycerides can be purified by treatment with activated carbon to remove organic impurities, alkali to remove unreacted glycerol esters, and ion exchange to remove salts. (wikipedia.org)
  • Haloferax volcanii , for example, is able to grow on glycerol and organic acids (Kauri et al. (springer.com)
  • product
  • needs update] Glycerol from triglycerides is produced on a large scale, but the crude product is of variable quality, with a low selling price of as low as 2-5 U.S. cents per kilogram in 2011. (wikipedia.org)