• In addition, we have used this method to decellularize 3D cultures of human gingival and skin fibroblasts 14,15 . (researchsquare.com)
  • Aims This is an investigation of potential oxidative effects of AGE in response to the antioxidant glutathione in cultured human gingival fibroblasts, using radiolabelled steroids as substrates and their product 5 alpha dihydrotestosterone (DHT) as a marker of wound healing.Methods Confluent monolayer cultures of human gingival fibroblasts were incubated with 14C-Testosterone and 14C-4-androstenedione respect. (endocrine-abstracts.org)
  • ZNF513 combined with KIF3C regulates gingival fibroblast proliferation, migration, and fibrosis response via the PI3K/AKT/mTOR and Ras/Raf/MEK/ERK pathways. (nature.com)
  • The fibroblasts were placed on these surfaces and cell proliferation evaluated using Trypan Blue stain. (bvsalud.org)
  • The aim of this in vitro study was to investigate the influence of three different scan sprays on the proliferation, viability, and adenylate kinase (ADK) release of human fibroblasts . (bvsalud.org)
  • METHOD AND MATERIALS Three different scan sprays (ScanDry, ScanSpray Luer Classic, and CEREC Optispray) were tested in vitro to determine their effects on proliferation, viability, and ADK release of human periodontal fibroblasts . (bvsalud.org)
  • There were differences between the scan sprays concerning inhibition of the proliferation and viability of fibroblasts . (bvsalud.org)
  • All materials inhibited the fibroblast proliferation and viability compared with the control group (P (bvsalud.org)
  • The inhibiting effect on the fibroblast proliferation can be attributed to a material-independent phenomenon. (bvsalud.org)
  • Due to the fact that many different results are described for different concentrations of alcohol at different times, an attemptwas made to visualise the direct impact of 7.2% and 22% alcohol on human gingival fibroblasts. (aaem.pl)
  • PANsystem 2000 was used for visualisation of the reaction of human gingival fibroblasts isolated from gingiva on ethanol in 2 different concentrations. (aaem.pl)
  • Mirror-polished surface of ceria-stabilized zirconia/alumina nanocomposite enhancement in the adhesion strength of human gingival fibroblasts. (alliedacademies.org)
  • In our findings, the expression or production of CXCL1 was abrogated after treating the human gingival fibroblasts with adenosine after IL-1β stimulation. (openrepository.com)
  • The antibacterial effects of six solutions on oral pathogen microorganisms were tested and their cytotoxic effects on human gingival fibroblasts were evaluated by MTT assay. (deu.edu.tr)
  • The aims of the study were to determine the in-vitro cytotoxicity and anti-inflammatory activities of common edible herbs and spices on cultured human gingival fibroblasts (HGFs). (benthamopen.com)
  • The oils from black pepper seed, clove bud and cinnamon bark showed dose-dependent inhibitory action on IL-6 on cultured bacterial Lipopolysaccharide (LPS)-induced human gingival fibroblasts. (benthamopen.com)
  • The aim of this study was to evaluate the role of cysteine proteinases in P. gingivalis colony growth and development, and subsequent immunomodulatory effects on human gingival fibroblast. (diva-portal.org)
  • Signaling pathways involved in the regulation of TNFα-induced toll-like receptor 2 expression in human gingival fibroblasts. (scilifelab.se)
  • In this study, we investigated the effects of the pro-inflammatory cytokine tumor necrosis factor α (TNFα) on TLR2 expression in human gingival fibroblasts. (scilifelab.se)
  • Influence of three scan spray systems on human gingival fibroblasts. (bvsalud.org)
  • The human gingival fibroblast cell line HGF‑1 was treated with various dilutions of an eluent solution of S‑PRG, which contained 32.0 ppm Al, 1,488.6 ppm B, 505.0 ppm Na, 12.9 ppm Si, 156.5 ppm Sr and 136.5 ppm F. Treatment with eluate at a dilution of 1:10,000 was observed to significantly promote the migration of HGF‑1 cells. (tokushima-u.ac.jp)
  • Our recent findings have shown that the widely used NH 4 OH/Triton X-100/DNase decellularization method is not able to completely remove cytoskeletal proteins from long-term three-dimensional human gingival fibroblast cultures. (researchsquare.com)
  • This decellularization method has been used in a variety of contexts including primary human dermal and lung fibroblast cultures, cancer-associated fibroblast cultures from esophageal, ovarian, renal, pancreatic, prostatic and lung tumors, and murine mammary fibroblasts 10,12,13 . (researchsquare.com)
  • Methods Normal human gingival fibroblast Gin-1 cells were used. (scirp.org)
  • Results: LED produced in vitro increases of cell growth of 140-200% in mouse-derived fibroblasts, rat-derived osteoblasts, and rat-derived skeletal muscle cells, and increases in growth of 155-171% of normal human epithelial cells. (researchgate.net)
  • In vitro cytotoxicity analysis of bioceramic root canal sealers on human gingival fibroblast cells. (iium.edu.my)
  • 2014. The effect of laser-treated titanium surface on human gingival fibroblast behavior. (bchi.lt)
  • iPS were generated via a platelet lysate or fetal bovine serum-based culturing protocol from matched dermal, buccal and gingival human fibroblasts, isolated from healthy donors ( n = 2) after informed consent, via episomal plasmid transfection. (biomedcentral.com)
  • P. gingivalis with Kgp affected the viability of gingival fibroblasts and modulated host inflammatory responses, including induction of TGF-β1 and suppression of CXCL8 and IL-6 accumulation. (diva-portal.org)
  • Our in vitro data from immunofluorescence results demonstrated the effect of adenosine in the inflammatory response of gingival fibroblasts, typified by the expression of CXCL8- family of cytokines, including CXCL1. (openrepository.com)
  • In this study, we investigated the in vitro effects of EMD on rat bone marrow stromal cells (BMSC) and gingival fibroblasts (GF). (tau.ac.il)
  • In vitro studies using infra-/red light have not shown any useful impact on wound healing by activating cells located in deeper skin layers such as fibroblasts or stem cells [2] . (researchgate.net)
  • Thus, adenosine may influence the stromal gingival inflammatory response in periodontitis by reducing the fibroblast-mediated activation of inflammatory cells. (openrepository.com)
  • Hereditary gingival fibromatosis (HGF) is a rare inherited condition with fibromatoid hyperplasia of the gingival tissue that exhibits great genetic heterogeneity. (nature.com)
  • Hereditary gingival fibromatosis (HGF) is an inherited disease characterized by fibrous overgrowth with pathological, nonhemorrhagic, diffuse or focal, slowly progressive hyperplasia of the gingival tissue, resulting in fibromatous hyperplasia of the gingival tissue. (nature.com)
  • Most HGF cases are nonsyndromic, with gingival hyperplasia as the single symptom. (nature.com)
  • Therefore, retaining the fibroblasts on the surface of a material is necessary to establish integration with soft tissue, and particularly for implant abutments sealing [ 7 ]. (alliedacademies.org)
  • Oral gingival fibroblasts serve a central role in maintaining oral mucosa homeostasis, and their functions include the coordination of physiological tissue repair. (tokushima-u.ac.jp)
  • Prevention of bacterial contamination is even more important in the case of gingival graft surgery because a significant amount of tissue is lost at a palatal donor site. (spandidos-publications.com)
  • Autogenous gingival grafts are often accompanied by discomfort, pain and retarded tissue repair depending on a patient's condition. (spandidos-publications.com)
  • 1989] obtained from two adult subjects undergoing routine periodontal therapies and who Notch-4 Proteins Purity & Documentation didn't have any form of gingival overgrowth. (gpr44.com)
  • ABSTRACT: Background: Among anti-hypertension drugs, calcium (Ca2+) antagonists cause gingival overgrowth as a side effect. (scirp.org)
  • Fixation and Sirius Red Assay The Sirius Red dye-binding assay for measuring collagen accumulation in gingival fibroblasts was adapted from a prior study completed in osteoblasts [Tullberg-Reinert and Jundt, 1999]. (gpr44.com)
  • The aim of this study is to investigate the antimicrobial properties of six propolis solutions and evaluate their cytotoxicity on gingival fibroblasts at different dilutions. (deu.edu.tr)
  • Mirror-polished Ce-TZP/Al 2 O 3 might contribute to the success of implant therapy by its strong adhesion strength to gingival connective tissues with the developed focal contact-cytoskeleton complex. (alliedacademies.org)
  • The composition, configuration, and density of the proteins in the pellicle derived from the saliva and gingival crevicular fluid are largely dependent on the physical and chemical nature of the underlying surface and thus the properties of the surface influence bacterial adhesion through the pellicle [ 2 ]. (hindawi.com)
  • The aim of the present study was to examine the effect of various concentrations of an S‑PRG filler eluate on the growth and migration of gingival fibroblasts. (tokushima-u.ac.jp)
  • Upregulation of Tβ 4 by cDNA-mediated transfection was found to cause actin depolymerization in fibroblast cells ( 1 ), which demonstrated that the modulation of Tβ 4 affected the polymerization state of actin cytoskeleton, as well as other cell processes associated with the organization of actin cytoskeleton, such as cell migration. (spandidos-publications.com)
  • The effective dilutions of the six propolis samples on periodontopathogen microorganisms were found to be cytotoxic to gingival fibroblasts. (deu.edu.tr)
  • All solutions had strong antifungal activity and the effective dilutions were safe for gingival fibroblasts. (deu.edu.tr)
  • Emdogain® (EMD), a formulation of Enamel Matrix Proteins (EMP), is used clinically for periodontal regeneration, where it stimulates cementum formation and promotes gingival healing. (tau.ac.il)
  • Soon after the cells had been grown to full confluence, the fibroblasts had been cultured inside the presence of among the solutions for 7 days, with three media alterations, or 6 days, with two media modifications, every single in the continuous presence of ascorbate, CTGF/CCN2 proteins and anti-CCN2/ CTGF antibodies. (gpr44.com)
  • It was our objective to study the role of mechanical stimulation on fibronectin (FN) reorganization and recruitment by exposing fibroblasts to shear fluid flow and equibiaxial stretch. (nature.com)
  • The fibroblasts grown from frozen stocks have been passaged twice for expansion, prior to becoming plated for experimental remedies at an initial concentration of 50,000 cells per effectively in 6-well plates or 25,000 cells per well in 12-well culture plates. (gpr44.com)
  • To this end, primary fibroblasts were seeded at a high density (25,000 cells per cm 2 ) and cultured for 7 days, as originally described for mouse NIH-3T3 cells, to generate 3D cultures 11 . (researchsquare.com)
  • Complimentary to this, fibroblasts exposed to fluid shear stress structurally rearranged pre-coated surface FN, but unstimulated and stretched cells did not. (nature.com)
  • Aiming to improve consumers' quality of life (QOL), we continue to build on this wealth of knowledge with research in such areas as non-destructive methods of treating dental caries, ways to reduce gingival inflammation based on molecular cytology, and the suppression of bacterial growth using proprietary biofilm models. (lion.co.jp)
  • Previous work in our lab has identified adenosine signaling as a key pathway able to control inflammatory chemokine secretion of gingival fibroblasts. (openrepository.com)
  • Morphologically, all iPS generated from fibroblasts formed tight colonies surrounded by a reflective "whitish" outer rim, typical for iPS. (biomedcentral.com)
  • Microarray analysis and the inhibition of specific signaling pathways demonstrated that c-Jun N-terminal kinases (JNK) and nuclear factor kappa B (NF-κB) were involved in the regulation of TNFα-induced TLR2 expression in gingival fibroblasts. (scilifelab.se)
  • The formation of a biological seal around the neck of titanium (Ti) implants is critical for ensuring integration at the gingival site and for preventing bacterialcolonization that may lead to periimplantitis. (upc.edu)
  • Bioactive polyphenol antioxidants protect oral fibroblasts from ROS-inducing agents. (aaem.pl)
  • In the present study, we undertook experiments to determine if TRPV1 channels are present in gingival fibroblasts and to ascertain if nifedipine-activated NSCCs are TRPV1 channels. (scirp.org)
  • From another side, fibroblasts are important in connective tissues production, remodelling, and creating a peri-implant barrier [ 6 ]. (alliedacademies.org)
  • Essential oils of selected herbs and spices retained compatibility with gingival fibroblasts in culture and showed inhibitory activity on IL-6 released by LPS-induced HGFs. (benthamopen.com)
  • The impact of the antioxidant glutathione (G) is investigated to clarify mechanisms involved, using 5 alpha dihydrotestosterone (DHT) as a hormone marker of healing.MethodsHuman gingival fibroblasts in confluent culture were incubated in Eagle s MEM, with 14C-testosterone / 14C-4-androstenedione an. (endocrine-abstracts.org)
  • ZNF513 , a transcription factor, binds to KIF3C exon 1 and participates in the positive regulation of KIF3C expression in gingival fibroblasts. (nature.com)
  • A widely used, alkaline detergent-based decellularization method has been developed for 3D NIH-3T3 mouse embryonic fibroblast cultures 10,11 . (researchsquare.com)
  • A defined amount of the test material was sprayed into 96 multiwell plates, dried, and incubated with fibroblasts . (bvsalud.org)
  • The obtained results suggest that daily usage of 7.2% alcohol contained in mouthwashes is non-toxic for gingival fibroblasts, and could be recommended after periodontal surgery. (aaem.pl)
  • Since the disease is prone to recurrence after surgery, HGF patients must undergo repeated surgical procedures of gingival resection, which brings a heavy psychological and economic burden to HGF patients and their families. (nature.com)