• strains
  • The R6Kγ replication origin allows the plasmid to replicate in pir + E. coli strains, and the kanamycin resistance marker allows for plasmid selection. (jove.com)
  • The strains were further analyzed in regard to their susceptibility to 35 antibiotics and to 34 different and newly isolated bacteriophages from the environment. (jove.com)
  • A structurally transformed lytic bacteriophage having a broad host range of Staphylococcus aureus strains and a penicillin-binding protein (PBP 2a) antibody conjugated latex beads have been utilized to create a biosensor designed for discrimination of methicillin resistant (MRSA) and sensitive (MSSA) S. aureus species 1,2 . (jove.com)
  • host
  • While Trichopria drosophilae infect the pupal stages of the host ( Fig. 1A-C ), females of the genus Leptopilina ( Fig. 1D, 1F, 1G ) and Ganaspis ( Fig. 1E ) attack the larval stages. (jove.com)
  • complex
  • We describe the use of Bio-layer Interferometry to study inhibitory interactions of subunit ε with the catalytic complex of Escherichia coli ATP synthase. (jove.com)
  • This process is also challenging given the substantial differences between E. coli and most original hosts responsible for complex natural product formation. (jove.com)
  • The assessment assays establish erythromycin A biosynthesis from E. coli and set the stage for future engineering efforts to improve or diversify production and for the production of new complex natural compounds using this approach. (jove.com)
  • method
  • Here we present a novel method for assessing the in vitro replication of HIV-1 as influenced by the gag gene isolated from acute time points from subtype C infected Zambians. (jove.com)
  • cell
  • Further, we utilize the gp120 aptamer-siRNA chimeras for cell-type specific delivery of the siRNA into HIV-1 infected cells. (jove.com)
  • Once transferred, the E. coli cell must support eventual biosynthesis. (jove.com)
  • In the case of erythromycin A, the E. coli cell had to be engineered to provide the two precursors (propionyl-CoA and (2S)-methylmalonyl-CoA) required for biosynthesis. (jove.com)