• Polymerase
  • Key words: Randomly amplified polymorphic DNA, Polymerase chain reaction, Lycopersiconesculentum. (thefreelibrary.com)
  • The former DNA sequencing method is based on chemical modification of DNA and subsequent cleavage at specific bases [ 8 ], while the latter method makes use of the 2',3'-dideoxy and arabinonucleoside analogues of the normal deoxynucleoside triphosphates, which act as specific chain-terminating inhibitors of DNA polymerase [ 9 ]. (ijbs.com)
  • genome
  • The specific information that is required determines which techniques are used and which parts of the genome are analysed. (wikipedia.org)
  • After reviewing mapping and next-generation sequencing methods, we would like to share our thoughts with the genome community on how to combine the HAPPY mapping technique with the new-generation sequencing, thus integrating two systems into one pipeline, called HAPPY pipeline. (ijbs.com)
  • The idea of mapping a genome became a reality in the mid-1970s, due to recombinant DNA technology that helped develop a variety of strategies to facilitate mapping. (ijbs.com)
  • In situ hybridization allowed scientists to construct a genome-wide physical map by using DNA probes to directly localize, orientate, and order genes [ 2 ]. (ijbs.com)
  • NUMT, pronounced "new might," is an acronym for "nuclear mitochondrial DNA segment" coined by evolutionary geneticist, Jose V. Lopez, which describes a transposition of any type of cytoplasmic mitochondrial DNA into the nuclear genome of the eukaryotic organisms. (wikipedia.org)
  • Not only has any correlation been found between the fraction of noncoding DNA and NUMT abundance in the genome but NUMTs are also proven to have non-random distribution and a higher likelihood of being inserted in the certain location of genome compare to others. (wikipedia.org)
  • The possible presence of organelle DNA inside the nuclear genome was suggested after finding of homologous structure to the mitochondrial DNA, which was shortly after the discovery of the presence of an independent DNA within the organelles in 1967. (wikipedia.org)
  • The searching for mtDNA in nuclear DNA continued until 1994 when the recent remarkable transposition of 7.9 kb of a typically 17.0-kb mitochondrial genome to a specific nuclear chromosomal position in the domestic cat was reported. (wikipedia.org)
  • populations
  • Outbreeding depression Fragmented populations Taxonomic uncertainties, which can lead to a reprioritization of conservation efforts Genetic drift as the main evolutionary process, instead of natural selection Management units within species Specific genetic techniques are used to assess the genomes of a species regarding specific conservation issues as well as general population structure. (wikipedia.org)
  • mitochondrial
  • After the release of the mtDNA to the cytoplasm, due to the mitochondrial alteration and morphological changes, mtDNA is transferred into the nucleus by one of the various predicted methods and are eventually inserted by double-stranded break repair processes into the nuclear DNA. (wikipedia.org)
  • Despite the metabolic alterations and functional adaptations in the host eukaryotes, circular mitochondrial DNA is contained within the organelles. (wikipedia.org)
  • Containing 37 genes, mitochondrial DNA has an essential role in the production of necessary compounds, such as required enzymes for the proper function of mitochondria. (wikipedia.org)
  • The integration and recombination of cytoplasmic mtDNA into the nuclear DNA is called Nuclear Mitochondrial DNA, which is abbreviated as NUMT. (wikipedia.org)
  • region
  • In addition, BLAST results showed high similarities in the partial mgc2 gene region between the South African and Zimbabwean genotypes and the 'atypical' Israeli RV-2 strain, suggesting a link in its epidemiology. (scielo.org.za)
  • 1980s
  • The HAPPY ( HAP loid DNA samples using the P ol Y merase chain reaction) technique was developed in the late 1980s [ 5 ]. (ijbs.com)
  • length
  • This technique took advantage of logarithmic spacing, and could be used to identify target bands ranging over a length of 20,000 nucleotides. (wikipedia.org)
  • development
  • The latter technique has proved to be less deletereous for the embryo, therefore it is advisable to perform the biopsy around day 5 or 6 of development. (wikipedia.org)
  • methods
  • DNA sequencing methods were also developed in the early 1970s, including Maxam-Gilbert sequencing and Sanger termination sequencing. (ijbs.com)
  • high
  • This includes, screening of high yielding genotypes based on their chemical profile, propagation of these genotypes using biotechnological tools, comparison of micropropagated plants with the mother plants for consistency of chemical and genetic profiles and the utility of micropropagation in the conservation of elite clones for future use. (springer.com)
  • years
  • Handyside, Kontogianni and Winston's first successful tests happened in October 1989, with the first births in 1990 though the preliminary experiments had been published some years earlier. (wikipedia.org)
  • unique
  • The South African genotype contained unique mgc2 and pvpA gene regions, while the Zimbabwean genotype proved to be even more distinct with unique gapA, mgc2 and pvpA gene regions. (scielo.org.za)
  • regions
  • Genotypes for 25 of these and 14 additional sites in the flanking regions were determined for the 57 NIL and associated with variation in bristle number in four genetic backgrounds. (genetics.org)
  • time
  • To be classified as such, morphs must occupy the same habitat at the same time and belong to a panmictic population (one with random mating). (wikipedia.org)
  • allows
  • Historic DNA is important because it allows geneticists to understand how species reacted to changes to conditions in the past. (wikipedia.org)
  • specific
  • The term preimplantation genetic screening (PGS) is used to denote procedures that do not look for a specific disease but use PGD techniques to identify embryos at risk. (wikipedia.org)
  • study
  • A total of 67 MG field genotypes from the USA, Israel and Australia, and 10 reference strains were characterised in the study. (scielo.org.za)
  • order
  • After this initial observation, Ellis coined the name "promiscuous DNA" in order to signify the transfer of DNA intracellularly from one organelle to the other and is the presence of organelle DNA in multiple cellular compartments. (wikipedia.org)