• transgenic mice generated by using BAC clone, cTG: conditional transgenic mice, KO: knockout mice, KI: knock-in mice, cKO: conditional knockout mice, CRIPR/Cas9: Endonuclease-mediated mice. (riken.jp)
  • Her first work in the lab helped to elucidate the sequence diversity of 20 inbred mouse strains based on substitutions, insertions, and deletions. (wikipedia.org)
  • PCR tests for the genetic background of inbred and wild-derived strains by using microsatellite or single nucleotide polymorphism (SNP) markers. (riken.jp)
  • Sequence analysis of genomic region or mutated gene: Sequencing of transgenes, targeted genes or any other mutated genes of TG, BAC-TG, cTG, KO, KI, CRISPR/Cas9, cKO and spontaneous/ENU-induced mutant strains. (riken.jp)
  • Since regulation of imprinted genes was thought to be governed by the epigenetic modifications to the imprinting control region (ICR), Ideraabdullah explored whether there are regions outside the ICR that are important in the regulation of imprinted genes. (wikipedia.org)
  • To perform a linkage analysis of the responsive gene, Mus musculus castaneus (CAST) (Jackson Laboratories, Bar Harbor, Maine) was chosen for the back-cross since it is relatively distant from the Jcl:ICR strain and there is abundant information about its microsatellite markers. (iiarjournals.org)
  • Background: We have established a mouse model of spontaneous deafness by sib-inbreeding over 10 years. (iiarjournals.org)
  • The genes relevant to deafness have been identified from the analysis of models of spontaneous deaf mice and many genes from such mice have been reported. (iiarjournals.org)
  • We established a spontaneous deaf mouse more than 10 years ago and the mouse has been reported in this journal ( 2 ). (iiarjournals.org)
  • Her thesis worked explored the genetic architecture of DDK syndrome and the genetic basis for embryonic lethality in mice. (wikipedia.org)
  • Materials and Methods: In order to identify the genetic abnormality, the mouse was back-crossed to Mus musculus castaneus (CAST), and myosine 15 or myoXV on chromosome 11 was assumed to be the responsive gene. (iiarjournals.org)
  • The mouse was bred with the mutant deaf mouse and offspring (the first generation, F1) mice were obtained. (iiarjournals.org)
  • F1 mice were then back-crossed to the original mutant deaf mice and hybrid F2 generation mice were obtained. (iiarjournals.org)
  • Please confirm the list and explanations of performed, not-performed and supplemental QC tests for mouse resources in each distribution format. (riken.jp)
  • She mutated different regions within the ICR for H19/IGF2 and this caused a loss of repression of the imprinted gene, yet the methylation patterns at the imprinted gene's ICR and promoter region did not change. (wikipedia.org)
  • METHODS: BCAA-fed mice and megakaryocyte/platelet-specific branched-chain α-keto acid dehydrogenase E1α subunit-deficient mice were used to study the role of BCAAs in thrombocytopoiesis. (bvsalud.org)
  • In this study, we used mice lacking the SR-A (SRKO) to investigate the role of SR-A in acquired immunity using a viable bacillus Calmette Guérin (BCG) infection model. (ox.ac.uk)
  • In this study, the effect of TWK10 on the progression of age-related impairments was investigated in mice. (frontiersin.org)
  • Considering the function that the affected area regulates and previous reports, hearing loss of the examined mouse is attributable to the abnormality of the myoXV gene and this mouse might be another type of shaker-2 deaf mouse. (iiarjournals.org)
  • We found that TWK10 not only enhanced muscle strength in young mice, but also prevented the aging-related loss of muscle strength in aged mice, which was accompanied by elevated muscle glycogen levels. (frontiersin.org)
  • Unravelling the chemical basis of competitive scent marking in house mice. (phbuffers.org)
  • Deposited mice are housed separately based on the microbial test results at deposition, and then, all the mice are cleaned-up by a rederivation procedure. (riken.jp)
  • genetic analysis of liver tumorigenesis in sv40 t antigen transgenic mice implies a role for imprinted genes. (liverpool.ac.uk)
  • liver tumors from interspecific hybrid, transgenic mice containing the sv40 early region linked to a mouse major urinary protein enhancer/promoter were analyzed for loss of heterozygosity to identify chromosomal regions which potentially contain genetic loci involved in multistep tumorigenesis. (liverpool.ac.uk)
  • As mouse ESCs are highly amenable to genetic manipulation and are capable of colonizing the germline of chimaeric mice [ 5 ], they are widely used for the generation of transgenic mice. (biomedcentral.com)
  • Given the early embryonic lethal phenotype of Dnmt3b knockout (KO) mice, ES cells could be a powerful tool for tracking and comparing the de novo methylation activity of DNMT3s during embryonic development (Fig. 1a ). (nature.com)
  • Knockout mice are used as EXPERIMENTAL ANIMAL MODELS for diseases ( DISEASE MODELS, ANIMAL ) and to clarify the functions of the genes. (lookformedical.com)
  • In this study, we examined differences in the stress response between FGF21 knockout (KO) mice and wild-type (WT) mice after social defeat stress (SDS). (tokushima-u.ac.jp)
  • She mutated different regions within the ICR for H19/IGF2 and this caused a loss of repression of the imprinted gene, yet the methylation patterns at the imprinted gene's ICR and promoter region did not change. (wikipedia.org)
  • C57BL mice are a commonly used strain of laboratory mice that are inbred to produce consistent and predictable results in scientific research. (lookformedical.com)
  • Male C57BL/6 J mice were separated into various PCMS groups: a control group with sawdust on the floor of the rearing cage (C), a group with mesh wire on the floor (M), and a group with water just below the mesh wire (W). Activation of lateral hypothalamic orexin neurons was assessed using immunofluorescence. (tokushima-u.ac.jp)
  • only three haplotypes are found in laboratory mice: ga, gb, and gc which are represented by balb/c, akr, and dba/2 prototypes respectively. (liverpool.ac.uk)
  • BALB/C is a commonly used strain of inbred mice in medical research, known for their genetic uniformity and susceptibility to various diseases. (lookformedical.com)
  • Genomic imprinting is an epigenetic process resulting in the expression of genes in a parent-of-origin specific manner. (biomedcentral.com)
  • the hsr on chromosome 1 of the house mouse, mus domesticus: distribution and frequency in switzerland. (liverpool.ac.uk)
  • a total of 357 house mice (mus domesticus) from 83 localities uniformly distributed throughout switzerland were screened for the presence of a homogenously staining region (hsr) on chromosome 1. (liverpool.ac.uk)
  • Full-length genomic sequence confirmed MWV as an arenavirus related distantly to Mobala, Mopeia and Ippy viruses, all members of the Old World arenavirus complex. (nih.gov)
  • cloning and sequence analysis of the gene and cdna encoding mouse spermidine/spermine n1-acetyltransferase--a gene uniquely regulated by polyamines and their analogs. (liverpool.ac.uk)
  • As homozygous genomic regions of PGA-derived cells are not compatible with allele-specific RNA-seq, we developed an RNA-seq-based genotyping strategy allowing identification of informative heterozygous regions. (biomedcentral.com)
  • the hepatic vitamin k epoxide reductase in the bromadiolone-resistant mice and in one phenotype of warfarin-resistant mice was highly insensitive to in vitro inhibition by warfarin and bromadiolone. (liverpool.ac.uk)
  • phenotype and tcr gamma delta variable gene repertoire of intestinal intraepithelial lymphocytes in wild mice (mus musculus domesticus): abundance of v gamma 1 transcripts and extensive delta gene diversity. (liverpool.ac.uk)
  • ga and gc haplotypes are the most frequent among laboratory mice whereas gb is poorly represented. (liverpool.ac.uk)
  • Imprinted gene dysregulation in a Tet1 null mouse model is stochastic and variable in the germline and offspring. (nih.gov)
  • Thus, we define a discrete set of genomic regions that require TET1 for germline reprogramming and discuss mechanisms for stochastic imprinting defects. (nih.gov)
  • Cloned cells in which this DNA alteration is successful are then injected into mouse EMBRYOS to produce chimeric mice. (lookformedical.com)
  • Here, we examined ICR-specific DNA methylation in Tet1-/- germ cells and ascertained whether abnormal ICR methylation impacted imprinted gene expression in F1 hybrid somatic tissues derived from Tet1-/- eggs or sperm. (nih.gov)
  • Somatic cell reprogramming has generated huge curiosity after the primary report by Yamanaka and his coworkers in 2006 on the technology of induced pluripotent stem cells (iPSCs) from mouse fibroblasts. (aprofarm.org)
  • Right here we report the technology of secure iPSCs from mouse fibroblasts by recombinant protein transduction (Klf4, Oct4, Sox2, and c-Myc), a process designed to bypass the dangers brought on by integration of exogenous sequences within the goal cell genome related to gene supply programs. (aprofarm.org)
  • The importance of imprinting during embryonic development is further underpinned by the fact that androgenetic mice (derived from two paternal pronuclei) and gynogenetic mice (derived from two maternal pronuclei) are embryonic lethal [ 13 , 14 ]. (biomedcentral.com)
  • The guanine quadruplex (G4) construction in DNA is a secondary construction motif that performs vital roles in DNA replication, transcriptional regulation, and upkeep of genomic stability. (aprofarm.org)
  • Era of mouse induced pluripotent stem cells by protein transduction. (aprofarm.org)
  • The cells had been handled with purified proteins 4 instances, at 48-h intervals, and cultured on mitomycin C handled mouse embryonic fibroblast (MEF) cells in full embryonic stem cell (ESC) medium till colonies fashioned. (aprofarm.org)
  • mouse la-4 lung adenoma cells treated with either spermine or the spermine analog, n1,n12-bis(ethyl)spermine, produced a 2.3 and 6.5-fold increase, respectively, in ssat mrna. (liverpool.ac.uk)
  • The genomic enrichment patterns of DNMT3A differ from those of DNMT3B in mouse embryonic stem (ES) cells, suggesting that each de novo methyltransferase has unique targets during development 14 , 15 . (nature.com)
  • To comprehensively identify the target sites for de novo DNA methylation by the DNMT3 enzymes, in the present study, we took advantage of female mouse ES cells established in the presence of MEK and Gsk3 inhibitors, which lack most DNA methylation (2i/L ES cells) 16 . (nature.com)
  • It should be noted, however, that mouse ES cells cultured in serum and leukemia inhibitory factor (LIF) (S/L ES cells) exhibit global DNA hypermethylation relative to inner cell mass (ICM) cells, which are the in vivo counterpart of ES cells 17 . (nature.com)
  • Although the genotype of the ESC-PGA cells is different from the female oocyte donor due to meiotic recombination occurring in the oocyte, subsequent matching of the ESC-PGAs based on the major histocompatibility complex antigens allows for engraftment of these cells in mouse recipients [ 10 ]. (biomedcentral.com)
  • The chimeric mice are then bred to yield a strain in which all the cells of the mouse contain the disrupted gene. (lookformedical.com)
  • Studies performed in mice indicated that the peripheral pathogenicity of LBV had been underestimated in previous studies. (cdc.gov)
  • Her findings were the first to suggest that mechanisms outside the ICR are necessary to mediate the fully repressed state of an imprinted gene. (wikipedia.org)
  • These data show that extracellular proteases and the quorum-sensing fsr system are important for E. faecalis virulence in two highly divergent hosts: nematodes and mice. (bcm.edu)
  • Recently, the Ten-Eleven Translocation proteins (TET1-3) have been shown to initiate DNA demethylation, with Tet1-/- mice exhibiting aberrant levels of imprinted gene expression and ICR methylation. (nih.gov)
  • Complementation of fsrB (TX5266) with a 6-kb fragment containing the entire fsr locus restored virulence in both the nematode and the mouse peritonitis models. (bcm.edu)