• Any process that activates or increases the frequency, rate or extent of DNA-directed DNA polymerase activity. (mcw.edu)
  • Chen CC, Simard MJ, Tabara H, Brownell DR, McCollough JA, Mello CC. A member of the polymerase beta nucleotidyltransferase superfamily is required for RNA interference in C. elegans. (umassmed.edu)
  • Edwards YJ, Carver TJ, Vavouri T, Frith M, Bishop MJ, Elgar G. Theatre: A software tool for detailed comparative analysis and visualization of genomic sequence. (umassmed.edu)
  • We sequenced and constructed the complete genome of an environmental strain CR1 of P. aeruginosa and performed the comparative genomic analysis. (frontiersin.org)
  • Comparative genomic analysis of the three candidate phage families revealed features without precedent in phage genomes. (biomedcentral.com)
  • Rare mutations in genome sequence that result from failed DNA repair are transmitted across generations through DNA replication during each cell division. (nature.com)
  • However, longer repetitive regions are only capable of being completely resolved by long reads of equal or greater size to the repeat, which makes input DNA quality a priority in sequence library construction. (biomedcentral.com)
  • Additionally, higher depths of coverage of long reads from current generation sequencing technologies are necessary to overcome high, relative error rates that can impact assembly quality and influence functional genomic annotation [ 12 ]. (biomedcentral.com)
  • tRNA nucleotidyltransferase (N2), proteasome subunit (EC 3.4.25.1) (N3), probable exosome complex RNA-binding proteins 1 (N4), huge ribosomal subunit proteins Retigabine tyrosianse inhibitor L37 Ae (N5) huge ribosomal subunit proteins L15electronic (N7), ribonuclease P (tRNA processing) proteins element 3 (EC 3.1.26.5) (N8), ribonuclease P protein element 2 (EC 3.1.26.5) (N9), prefoldin, chaperonin cofactor (N10), and a predicted exosome subunit containing the IMP4 domain within small nuclear ribonucleoprotein (N11). (cancercurehere.com)
  • METHODOLOGY/PRINCIPAL FINDINGS: Using total genomic DNA isolated from peripheral blood of confirmed VL cases (n = 40) and lesional biopsies of PKDL cases (n = 64), the kinetoplast-DNA based qPCR and RPA assay was performed and parasite load expressed as Cycle threshold (Ct) and Time threshold (Tt) respectively. (bvsalud.org)
  • ZIKV attenuates IFN-ß expression induced by DNA viruses (herpes simplex virus type 1, HSV-1) or two double-stranded DNAs (dsDNA90 and HSV120) in mouse embryonic fibroblasts (MEFs). (bvsalud.org)
  • Cytosolic double-stranded DNA surveillance by cyclic GMP-AMP synthase (cGAS)-Stimulator of Interferon Genes (STING) signaling triggers cellular senescence, autophagy, biased mRNA translation, and interferon-mediated immune responses. (bvsalud.org)
  • This CCA deactivation is reversible and quickly repairable by the CCA-adding enzyme [ATP(CTP):tRNA nucleotidyltransferase]. (prolekarniky.cz)
  • This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. (nih.gov)
  • This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus reducing the number of supercoils and altering the topology of DNA. (nih.gov)
  • Genomic information for outlier strains of Pseudomonas aeruginosa is exiguous when compared with classical strains. (frontiersin.org)
  • Repetitive content contributes to difficulty in multicellular Eukaryotic genome assembly as well [ 4 ], but the problem becomes more complicated in metagenome assembly [ 5 ] due to the wide range of abundance among bacterial species and strains, and the presence of other environmental DNA (e.g., plants, protists). (biomedcentral.com)
  • Significant genomic differences within the S. suis population, even amongst strains belonging to the same serotype, implied that virulence potential is genetically related. (biomedcentral.com)
  • In Cryptococcus neoformans , where changes in ancestral DNA methylation can potentially persist for millions of years, methyltransferases copy methylation patterns upon DNA replication 6 . (nature.com)
  • This can present a problem in metagenomic samples as material-adherent bacterial populations produce tough extracellular capsules that require vigorous mechanical stress for lysis, resulting in substantial DNA fragmentation and single-strand nicks [ 8 ]. (biomedcentral.com)
  • Here, we unexpectedly found that interferon regulatory factor 3 (IRF3), activated during innate DNA sensing, forms substantial endogenous complexes in the nucleus with retinoblastoma (RB), a key cell cycle regulator. (bvsalud.org)
  • To research tail structures on the genomic size we recently created a method known as TAIL-seq that deep-sequences the 3′ most fragments of RNAs (Chang et al. (researchtoactionforum.org)
  • TERT expression is associated with metastasis from thin primaries, exhausted CD4+ T cells in melanoma and with DNA repair across cancer entities. (nih.gov)
  • Centre for Genomic and Experimental Medicine, Medical Research Council Institute of Genetics and Molecular Medicine, The University of Edinburgh, Edinburgh, UK. (ox.ac.uk)
  • Genes belonging to proteases, bacterial exporters and DNA stabilization were found to be under strong positive selection, thus facilitating pathogenicity and survival of the outliers. (frontiersin.org)
  • The TRNT1 protein modifies tRNAs by adding a series of three DNA building blocks (nucleotides), called a CCA trinucleotide, to the molecule. (medlineplus.gov)
  • The protein encoded by the zif268 gene contains three tandemly repeated consen- sus zinc finger sequences, a feature found in the DNA- binding domains of a number of proteins that regulate tran- scription (21). (nih.gov)
  • We therefore set out to determine whether Zif268 is a sequence- specific DNA-binding protein, as suggested by its zinc finger sequence, and to identify the DNA site to which it binds. (nih.gov)
  • Summary Panel shows basic genome annotation information for the gene such as Locus tag, symbol, Protein product description and genomic coordinates etc. (systemsbiology.net)
  • [ 1 , 2 ] Genome sequence analysis of a number of multidrug-resistant A. baumannii clinical strains has shown the presence of several large genomic islands containing multiple resistance genes interspersed with transposons, integrons, and other mobile genetic elements. (medscape.com)
  • Replication stress conferred a therapeutic vulnerability to drugs targeting DNA-damage repair pathways. (nih.gov)
  • As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. (nih.gov)
  • Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb (PubMed:24310611). (nih.gov)
  • These histone PTMs influence chromatin organization and can regulate many DNA-templated processes such as transcription, replication, recombination, and repair. (unl.edu)
  • We report that Zif268 has one of the characteristics of a transcriptional regulator, namely, sequence-specific binding to DNA. (nih.gov)
  • Genome view will display genomic neighborhood information from Tuberculist. (systemsbiology.net)
  • The virion has a segmented genome consisting of three pieces of doubled-stranded DNA and also a unique lipid-containing envelope. (lookformedical.com)
  • Epigenetic mechanisms also play an essential role in defense responses against a variety of intracellular genomic parasites, such as viruses and transposable elements. (unl.edu)
  • Viruses whose nucleic acid is DNA. (lookformedical.com)
  • A temperate coliphage, in the genus Mu-like viruses, family MYOVIRIDAE, composed of a linear, double-stranded molecule of DNA, which is able to insert itself randomly at any point on the host chromosome. (lookformedical.com)
  • We are isolating, by a variety of genetic and functional genomic approaches, mutants defective in RNAi with the purpose of identifying new pathway components as well as characterizing their role(s) in controlling endogenous gene expression. (unl.edu)
  • Bacterial extracts used for DNA binding were prepared as described (23). (nih.gov)
  • The phenomenon by which a temperate phage incorporates itself into the DNA of a bacterial host, establishing a kind of symbiotic relation between PROPHAGE and bacterium which results in the perpetuation of the prophage in all the descendants of the bacterium. (lookformedical.com)
  • This gene silencing involves heritable (but reversible) epigenetic mechanisms, in contrast to genetic effects that are based on stable changes at the DNA level. (unl.edu)
  • In eukaryotes, nuclear DNA is associated with histones and other proteins in a complex structure referred to as chromatin. (unl.edu)
  • Host-Informed Expression of CRISPR Guide RNA for Genomic Engineering in Komagataella phaffii. (harvard.edu)
  • In addition to Distance from binding coordinate and genomic feature type of the target gene, differential expression and associated significance scores identified in TFOE experiments will also be shown here. (systemsbiology.net)
  • List of TF that were found to have binding peak close to genomic coordinates of this gene in ChIP-Seq experiments will be listed here together with differential expression and binding peak properties. (systemsbiology.net)
  • Enforced expression of Helios or Xbp1 elicited specific signatures, but Eos, Irf4, Satb1, Lef1 and Gata1 elicited exactly the same outcome, synergizing with FoxP3 to activate most of the Treg signature, including key TFs, and enhancing FoxP3 occupancy at its genomic targets. (gsea-msigdb.org)
  • Upon binding DNA, cGAS synthesizes a nucleotide signal 2'3'-cGAMP that activates STING-dependent downstream immunity. (nih.gov)
  • This in vitro translation product was used directly for DNA binding studies. (nih.gov)
  • Gel Retardation Assay for DNA Binding. (nih.gov)
  • Binding was carried out in 25 yl of 10 mM Tris, pH 7.5/50 mM NaCl/1 mM dithiothreitol/1 mM EDTA/ 10 uM ZnCl,/5% glycerol with sonicated salmon sperm DNA at 15-50 xg/ml. (nih.gov)
  • RNA interference is also becoming a valuable tool for loss-of-function high-throughput genomic studies in eukaryotes and for practical applications in animals, plants and algae. (unl.edu)
  • Has four distinct domains: an N-terminal nucleotidyltransferase (NT) domain responsible for UTase activity, a central HD domain that encodes UR activity, and two C-terminal ACT domains that seem to. (systemsbiology.net)
  • Its degradation in response to DNA damage is required for the inhibition of fork progression and cell survival (PubMed:24022480). (nih.gov)
  • The folding of an organism's DNA molecule into a compact, orderly structure that fits within the limited space of a CELL or VIRUS PARTICLE. (lookformedical.com)
  • Corynebacterium genus consists of Gram-positive aerobic or anaerobic facultatively pleomorphic rods with a high G + C content DNA. (biomedcentral.com)
  • It functions in the nucleoplasmic structure where it transcribes DNA into RNA. (harvard.edu)