Genomic 1998 random amplified polymorphic dna technique. Medical search. Frequent questions

Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
1984). Solanum demissum, phic DNA (RAPD) and restriction fragment length poly- a Mexican hexaploid species, has been used extensively morphism (RFLP) markers that are closely linked to the in potato breeding programs. (health-abstracts.com)
Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Single-Strand Conformation Polymorphism (SSCP) and Microsatellite DNA. (biomedcentral.com)
Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
The RAPD (Random Amplification Polymorphic DNA) allows the amplification of DNA sequences and is a simple and quick technique that does not require prior knowledge on the genomes to characterize organisms, using one randomly determined (usually a decamer) primer 17 . (bvsalud.org)

Amplified fragment length polymorphism (AFLP) markers were used to detect the relationships among 12 wild accessions and 62 groundcover chrysanthemum cultivars. (ashs.org)
alata ) using amplified fragment length polymorphism (AFLP) technique. (open-science-repository.com)

The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)

A total of 170 AFLP fragments were detected and all are polymorphic. (open-science-repository.com)

The increasing availability of DNA amplification by PCR at the beginning of the 1990s triggered a large number of studies using the amplification of microsatellites as genetic markers for forensic medicine, for paternity testing, and for positional cloning to find the gene underlying a trait or disease. (wikipedia.org)
A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
4, 5 The process involves use of many evenly spaced polymorphic DNA markers to create landmarks across each chromosome. (bmj.com)
The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
This review gives a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. (biomedcentral.com)
By the 1980s, many different types of DNA molecular markers had been explored, e.g. (biomedcentral.com)
All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
Molecular techniques have facilitated the identification, genotyping, and monitoring of food contamination sources, identification of genetically modified organisms, and use as food detection methods to avoid falsification through genetic markers. (peertechzpublications.com)
Residual heterozygosity in the initial inbreds can partly explain the observed responses as evidenced by 42 markers derived from both Methyl-Sensitive Amplification- and Amplified Fragment Length- Polymorphisms. (biomedcentral.com)

1992). A microsatellite is a tract of tandemly repeated (i.e. adjacent) DNA motifs that range in length from one to six or up to ten nucleotides (the exact definition and delineation to the longer minisatellites varies from author to author), and are typically repeated 5-50 times. (wikipedia.org)

It's been approximated that to anticipate the ancestry of people, up to thousand random one nucleotide polymorphisms (SNPs) or brief tandem repeats (STRs) may 864814-88-0 manufacture be required [13]. (tech-strategy.org)
Mitochondrial (mt) genomes, due to their properties to evolve faster than the nuclear DNA, to contain introns and mobile elements and to exhibit extended polymorphisms, are ideal tools to examine genetic diversity within fungal populations and genetically identify a species or a particular isolate. (biomedcentral.com)

Of the 444 nonreplicate imipenem-resistant P. aeruginosa isolates collected in 2001, a total of 89 were randomly selected and analyzed for acquired MBL genes of the bla IMP and bla VIM types in dot-blot hybridization experiments carried out with purified genomic DNA spotted (0.5 μg per spot) on positively charged nylon membranes (ZetaProbe, Bio-Rad, Hercules, CA) with digoxygenin-labeled DNA probes. (cdc.gov)
With the release of a genome wide SNPs database, it has become feasible to analyse these genetically determined QTLs for genes polymorphic in these strains. (bmj.com)
Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
The zebra finch MHC includes multiple Class I and Class II genes, some of which appear to be pseudogenes, and spans a much more extensive genomic region than the chicken MHC, as evidenced by the presence of MHC genes on each of seven BACs spanning 739 kb. (biomedcentral.com)
The major histocompatibility complex ( MHC ) is a gene-dense genomic region within which many genes play a role in vertebrate immune response. (biomedcentral.com)

We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. (lookformedical.com)

Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)

Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)

The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. (scielo.br)

They have a higher mutation rate than other areas of DNA leading to high genetic diversity. (wikipedia.org)
Mitochondrial DNA diversity of mud crab Scylla olivacea (Portunidae) in Peninsular Malaysia: a preliminary assessment. (mabjournal.com)
Genomic Diversity and Evolution of Rhizobia. (unifi.it)
Microbial Diversity in the Genomic Era, pp. 37-46 Elsevier, ISBN:9780128148495. (unifi.it)
We examined different methods for accessing genetic diversity and estimating genetic diversity parameters at the genomic level. (scirp.org)

Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
One could say the primary limitation to crop improvement is no longer, "can we make a precise sequence change in a precise location in eukaryotic genomic DNA? (frontiersin.org)

2000). The main advantage in this context is that the complex genetic and epigenetic correlations among interconnected parts of the brain can be explored using complementary molecular, developmental, structural, pharmacological, and behavioral techniques. (nervenet.org)

In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)

Comparative genomics analyses have shown a wide range of genomic variation within bacteria from different phylogenetic groups [1-3]. (researchsquare.com)

The small genomes of the DED fungi also facilitate genomic analyses. (sisef.it)

These species have elevated genomic complexities and limited genetic resources, hindering the application of marker-assisted selection strategies. (bvsalud.org)
However, due to the polyploidy nature of these polyploid species, more accurate models for incorporating genomic selection into breeding schemes are needed. (bvsalud.org)
Therefore, bacteria can present very different genomic compositions even within a species, with HGT creating a flexible genome and natural selection purging or maintaining it [10, 14]. (researchsquare.com)

Intriguingly, we found that the X chromosome breakpoint in the daughter could not be defined by a single breakpoint spanning genomic clone and further analysis showed a 650 kb submicroscopic duplication between DXS7067 and DXS7060 on either side of the X chromosome translocation breakpoint. (bmj.com)

Based on a combination of mitochondrial and nuclear DNA, the following questions were addressed: 1) the phylogeography of the two O. h. robertsoni phylogroups, 2) regional and local population structure in space and time, and 3) patterns of local dispersal under different isolation-by-distance scenarios. (biomedcentral.com)

Nineteen EcoR I/ Mse I primer combinations revealed 452 informative polymorphic bands with a mean of 23.8 bands and 71.5% polymorphic rate per primer pair. (ashs.org)
Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 Eco RI and Ms eI primer combinations. (open-science-repository.com)

Provision of broad, method-based PT schemes, such as DNA sequencing, would allow assessment of a large number of tests for which formal PT is not currently available. (cdc.gov)

In this context, the identification of such contaminants is important to avoid compromising further selection cycles, as well as genetic and genomic studies. (bvsalud.org)
Finally, we examined genomic studies on some endangered cattle breeds. (scirp.org)
Studies have been demonstrating the effectiveness of molecular techniques regarding the accuracy of the protocols used and the agility to provide the necessary answers. (peertechzpublications.com)

Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)

They are widely used for DNA profiling in cancer diagnosis, in kinship analysis (especially paternity testing) and in forensic identification. (wikipedia.org)
In forensics, mutation rates are needed when STRs are applied to paternity testing, and Y-STRs are especially powerful in deficiency cases of disputed paternity involving male offspring where the alleged father is not available for DNA analysis but is replaced by any of his male paternal relatives. (springer.com)

A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)

Although the first microsatellite was characterised in 1984 at the University of Leicester by Weller, Jeffreys and colleagues as a polymorphic GGAT repeat in the human myoglobin gene, the term "microsatellite" was introduced later, in 1989, by Litt and Luty. (wikipedia.org)
Xq28 appears to be an unstable region of the human genome and genomic rearrangements are recognised as major causes of two single gene defects, haemophilia A and incontinentia pigmenti, which map within Xq28. (bmj.com)
Some examples include delivering DNA-free gene editing reagents such as ribonucleoproteins or mRNA, relying on reagent expression from non-integrated DNA, using novel delivery mechanisms such as viruses or nanoparticles, using unconventional selection methods to avoid integration of transgenes, and/or avoiding tissue culture altogether. (frontiersin.org)
Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)

A microsatellite is a tract of repetitive DNA in which certain DNA motifs (ranging in length from one to six or more base pairs) are repeated, typically 5-50 times. (wikipedia.org)

We investigated the prevalence of intramolecular changes and of genomic reassortment among Sin Nombre viruses detected in deer mice in three western states. (biomedcentral.com)

DNA samples (10 µg) were digested with Eco Rl, Hin dlll, Eco RV, These three BC populations are designated 1K6, 1K27 and LB1, or Dra l at a DNA/enzyme ratio of 7 units per microgram. (health-abstracts.com)
The enzyme has a preference for double-stranded DNA. (lookformedical.com)

Of these, studying DNA-confirmed father-son pairs is the most reliable approach but only if the number of father-son pairs investigated is large enough to reveal reliable mutation rate estimates. (springer.com)

In recent decades, traditional methods of microorganism phenotyping have been replaced or added by the procedures associated to recombinant DNA 12-14 . (bvsalud.org)
For a long time, food analysis has always been based on physical, chemical, physicochemical, and biochemical methods, however, these are techniques that sometimes require a lot of time and have a low degree of precision. (peertechzpublications.com)

At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)

Only a small number of genomic regions in the BXN set deviate significantly from the expected 50:50 segregation ratio of alleles. (nervenet.org)

The application of molecular techniques in food is large, several authors comment on the detection and quantification of strains of pathogenic microorganisms present in foods and the presence of toxins and waste allergens not related to specific goods in production lines. (peertechzpublications.com)

This review aims to address the use of molecular techniques applied to the identification of food. (peertechzpublications.com)

Portions of the Sin Nombre virus small (S) and medium (M) RNA segments were amplified by RT-PCR from kidney, lung, liver and spleen of seropositive peromyscine rodents, principally deer mice, collected in Colorado, New Mexico and Montana from 1995 to 2007. (biomedcentral.com)

The establishment of reference populations for the major South African sheep breeds to estimate genomic breeding values is an immediate challenge. (scielo.org.za)

Currently, the most promising approach for increasing genetic gains in plant breeding is genomic selection. (bvsalud.org)

United Netherlands, and genomic ex-Royal ecosystems. (music-of-benares.com)

this allows them to accumulate mutations unhindered over the generations and gives rise to variability that can be used for DNA fingerprinting and identification purposes. (wikipedia.org)
Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification. (bvsalud.org)

In white blood cells, the gradual shortening of telomeric DNA has been shown to inversely correlate with ageing in several sample types. (wikipedia.org)

The name "satellite" DNA refers to the early observation that centrifugation of genomic DNA in a test tube separates a prominent layer of bulk DNA from accompanying "satellite" layers of repetitive DNA. (wikipedia.org)

The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. (mabjournal.com)

Organisms1

Chemicals and Drugs2

Analytical, Diagnostic and Therapeutic Techniques and Equipment3

Phenomena and Processes3

Anthropology, Education, Sociology and Social Phenomena1

Information Science3

RAPD11

Fragment length polymorphism2

Polymorphism1

AFLP1

Markers9

19921

Polymorphisms2

Genes5

Nucleotide2

Amplification1

Sequences2

Fragments1

Diversity5

Eukaryotic2

20001

Pathology1

Variation1

Analyses1

Species3

Chromosome1

Patterns1

Primer combinations2

ASSESSMENT1

Studies3

Organisms1

Paternity2

Approaches1

Gene4

Length1

Viruses1

Enzyme2

Mutation1

Methods2

Widely1

Regions1

Application1

Aims1

Small1

Major1

Selection1

Netherlands1

Identification2

Shown1

Early1

Samples1