Genomes motility proteins genes bacterial. Medical search. Frequent questions

The use of ancestral sequence reconstruction (ASR) and functional analysis of reconstructed stators shows promise for understanding how these proteins evolved and when the divergence in ion use may have occurred. (bvsalud.org)
Identification of a gene, pilV , required for type 4 fimbrial biogenesis in Pseudomonas aeruginosa , whose product possesses a pre-pilin-like leader sequence. (microbiologyresearch.org)
Architectural proteins, although minimally conserved in terms of structure and sequence among kingdoms, act upon DNA using conserved mechanisms (Luijsterburg et al. (universiteitleiden.nl)
Analysis of the Campylobacter phage CP8 genome identified significant sequence identity of protein gp010 with the avian leukocyte receptor CD30 ligand. (nottingham.ac.uk)
Sigma factors are a class of proteins constituting essential dissociable subunits that confer sequence-specific DNA binding properties to RNA polymerase (RNAP). (hindawi.com)
We detected the potential link between observed effects and insertion sequence element-induced changes in gene context only for a few genes. (cdc.gov)
200 copies of insertion sequence 481 (IS481) in its genome ( 14 ). (cdc.gov)
The amino acid sequence of LI0570 exhibited similarities to flagellar-associated proteins in four different bacterial strains. (biomedcentral.com)
We propose under SeqCode the complete genome sequence of strain M17CTs (CP122380) as a nomenclatural type of Bathyarchaeum tardum, which should be considered as a type for the genus Bathyarchaeum, which is proposed as a type for the family Bathyarchaeaceae, order Bathyarchaeales, and of the class Bathyarchaeia. (uibk.ac.at)
In a typical REU project, students analyze sequence data to identify mutations that could influence the evolution of protein function, then construct the mutations, express, purify and assay the enzymes to determine the role of the mutated position. (tamu.edu)
We define a methodology to investigate amplifications in bacterial genomes based on read depth of genome sequence data as a proxy for copy number. (cdc.gov)
We demonstrate the approach with Bordetella pertussis, whose insertion sequence element-rich genome provides extensive scope for amplifications to occur. (cdc.gov)

In addition, pyrD inactivation reduced flagellar motility and strongly affected biofilm formation by downregulating transcription of both type 1 fimbriae and curli subunit genes. (rossimicrolab.com)
The molecular level effects on motility, attachment/invasion of eukaryotic cell lines, and biofilm formation. (usda.gov)
Sub-objective 4.1: Analysis of ExPEC isolated from chickens and humans: biofilm assays, virulence gene profiles, antimicrobial resistance profiles, whole genome comparison of ExPEC strains isolated from chicken and human infections. (usda.gov)
These colony variants show great differences in biofilm formation and motility. (hindawi.com)
This indicates that the rpoX gene is involved in biofilm formation and stress response but the effects are controlled by colony phase variation in V. alginolyticus . (hindawi.com)
Based on this, we wanted to identify this protein in V. alginolyticus ZJ-51 and study whether it has a role in biofilm formation and stress response in the two colony phase variants. (hindawi.com)
2. Bacteria use quorum sensing to monitor population density and regulate group behaviors such as virulence, motility, and biofilm formation. (asu.edu)
MAGs were further used to analyze if genes involved in AMR and biofilm formation of viable microbes in ISS have variation due to generational evolution in microgravity and radiation pressure. (biomedcentral.com)
Despite many insights gained on the structure and functions played by flagellum and pili in motility, biofilm formation, surface attachment and interactions with bacteriophages, we know little about how these appendages are regulated in Xcc . (biomedcentral.com)

Thus, molecular mechanisms of actin-based motility by which other intracellular bacterial pathogen spread in the infected cells may differ from those adapted by LI [ 4 ]. (biomedcentral.com)
The key module of NeMeSys is an ordered and complete collection of mutants with mutations in each non-essential gene, the only such collection in a bacterial pathogen. (cnrs.fr)
We have used NeMeSys to shed light on the functions of multiple genes, and we expect this toolbox to allow, for the first time, the global functional profiling of a major human bacterial pathogen. (cnrs.fr)
This bacterial pathogen represents a prospective biological control agent for important nematode parasites. (biomedcentral.com)
Barrier agains t pathogen s gut bacterial genes in the MetaHIT ation when attempting to extrapolate [10], although Proteobacteria, Ver- catalogue were also well represent- results obtained in mouse models to rucomicrobia, and Fusobacteria are ed in the other metagenomes that the situation in humans. (who.int)

L. agilis BKN88 was more sensitive to bile and acid than the gut-derived non-motile lactobacilli, implying that L. agilis might utilize motility and chemotaxis instead of exhibiting stress tolerance/resistance. (nature.com)
Chemotaxis is triggered by the binding of ligands (chemoeffectors) to chemoreceptors called methyl-accepting chemotaxis proteins (MCPs) and transducer-like proteins (Tlps). (nature.com)
By transcriptome analyses (RNA-seq and RT-PCR) we confirm these observations as PilG is shown to upregulate many genes involved chemotaxis and flagellar biosynthesis but these similar genes were downregulated by PilH. (biomedcentral.com)

Leptospira biflexa are a helix shaped bacterium containing periplasmic flagella that allow for high motility and can contribute to virulence in pathogenic species and colonization of diverse environments. (wikipedia.org)
Leptospira biflexa are a species of genus Leptospira consisting of pathogenic and free living saprophytic bacterial species. (wikipedia.org)
Due to the fast growth rate and ease of genetic manipulation the use of this model bacterium species allows insight to gene functions within Leptospira. (wikipedia.org)
In this study, we undertook extensive genome-wide comparative analyses of twelve species that conform the Pectobacterium genus. (mdpi.com)
This chemotactic signaling pathway is relatively conserved across bacteria, whereas chemoeffectors and chemoreceptors vary among bacterial species/strains. (nature.com)
Here we review the most recent advancements on C. concisus and other human hosted Campylobacter species including their clinical relevance, transmission, virulence factors, disease associated genes, interactions with the human immune system and pathogenic mechanisms. (frontiersin.org)
Some of these animal hosted Campylobacter species, such as Campylobacter jejuni and Campylobacter coli , can cause acute bacterial gastroenteritis in humans through consumption of contaminated food or water ( Galanis, 2007 ). (frontiersin.org)
Bacterial species are usually not harmful to their hosts, although there are exceptions. (frontiersin.org)
In lots of bacterial species, such as and and display that it is required for efficient predatory growth. (bibf1120.com)
Among 46 MAGs retrieved, 18 bacterial species were identified, including one novel genus/species combination ( Kalamiella piersonii ) and one novel bacterial species ( Methylobacterium ajmalii ). (biomedcentral.com)
Phylogenetic analyses of five bacterial species showed ISS-specific evolution. (biomedcentral.com)
One of our long-standing goals has been to create such a resource in N. meningitidis - one of the most feared human bacterial pathogens that causes meningitis and septicaemia - and to use it for large-scale functional profiling of this species genome. (cnrs.fr)
The picture is si-milar in Vibrio - 'similar' refers to a great deal of protein homology among the numerous components in various species, despite vastly different regulatory circuits - for which the present model for competence involves 19+ genes, and, as core components: 1. (asmblog.org)
The microbiome, the "other ge- species have no representative in nome" or "second genome" of the culture collections. (who.int)
A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)

Leptospira biflexa is a spirochaete bacterium in the genus Leptospira and was the first saprophytic Leptospira genome to be sequenced. (wikipedia.org)
The entire consortium swims towards the light (scotophobic response), which the epibionts need for anoxygenic photosynthesis, whereas the central bacterium provides the necessary motility. (dsmz.de)
There are many ions which can be used by the BFM stators to power motility and different ions can be used by a single bacterium expressing multiple stator variants. (bvsalud.org)
Proteins/genes with a putative catabolic role and bacterium adaptation mechanisms during OPP degradation were identified via genomic and proteomic analysis. (nature.com)
The bacterium had its genome completely sequenced because it contains a unique extracellular enzyme system capable of breaking down insoluble cellulose into ethanol which is vital for biomass energy. (kenyon.edu)

Whole-genome sequencing showed that historic and recent isolates of B. pertussis have substantial variation in genome organization and form separate phylogenetic clusters. (cdc.gov)
Comparative analyses of MAGs and whole-genome sequences of related ISS isolates and their type strains were characterized to understand the variation related to the microbial evolution under microgravity. (biomedcentral.com)

We will use omic technologies to analyze a large variety of strains of each of the pathogens to identify genes and proteins necessary for pathogens to survive stresses encountered in food environments and cause human illness. (usda.gov)
Interestingly, some enteric bacterial pathogens have mechanisms to penetrate the mucus layer to reach epithelial cells via flagella-driven motility which plays a role in the initial phase of infection [ 5 , 6 ]. (biomedcentral.com)
which has undergone significant genome decay shedding the capability for success in the surroundings (3 9 Despite their divergent niche categories and web host predilections are facultative intracellular pathogens that display nearly similar intracellular lifestyle cycles (10). (healthandwellnesssource.org)
Next-generation therapies against bacterial infections will result from rational exploitation of the genomic sequences of bacterial pathogens, and from a better molecular understanding of their virulence. (cnrs.fr)
Our second research stream focuses on type 4 filaments (T4F), key virulence factors in numerous bacterial pathogens. (cnrs.fr)
T4F have been studied for decades because they are key virulence factors in many human bacterial pathogens. (cnrs.fr)

Recently, genome sequences revealed that in addition to rpoS , an rpoS -like sigma factor was found in V. alginolyticus 12G01. (hindawi.com)
Because ''C. thermocellum'' is known as a degrader of cellulose, its DNA contains specific nucleotide sequences that make up the genes that encode for the system of enzymes that are necessary for cellulose degradation. (kenyon.edu)
In contrast, the 16S rRNA gene sequences from 158T and 78-1T were only 95 and 90? (woofahs.com)
Since microbial virulence increases in microgravity, AMR gene sequences of MAGs were compared with genomes of respective ISS isolates and corresponding type strains. (biomedcentral.com)
By revealing complete repertoires of genes, genome sequences have provided the key to better, and eventually holistic, understanding of the biology of all living organisms. (cnrs.fr)

Symbiosis genes" of the epibiont were identified by genomic approaches. (dsmz.de)
On the basis of these observations, we hypothesized that strains with different genomic organization should display altered global transcriptomic and, consequently, proteomic profiles, and thereby genome rearrangements might contribute to strain variation and adaptation. (cdc.gov)
To further investigate the genomic diversity among this group and to help characterize lineages of the plague organism that have no sequenced members, we present here the genomes of two isolates of the "classical" antiqua biovar, strains Antiqua and Nepal516. (asm.org)
However, metagenome-assembled genomes (MAGs) of ISS samples are yet to be generated and subjected to various genomic analyses, including phylogenetic affiliation, predicted functional pathways, antimicrobial resistance, and virulence characteristics. (biomedcentral.com)

L. agilis BKN88 contains five putative chemoreceptor genes ( mcp1 - mcp5 ). (nature.com)
Putative large exoproteins and a putative protein with a RTX toxin-type ß-roll could be identified. (dsmz.de)
Transcription analysis of all putative catabolic genes established their role in the metabolism of OPP. (nature.com)
In this work, we investigated the antigenic and functional characteristics of a putative flagellar-associated protein, LI0570, using in silico computational approaches for epitope prediction and an in vitro protein-based molecular assay. (biomedcentral.com)

Bacterial Two Crossbreed (BTH) analysis displays DivIVA may connect to proteins that react to metabolic indications of amino-acid biosynthesis or adjustments in redox condition. (bibf1120.com)
The two variants exhibit different capability to form biofilms and to express genes involved in polysaccharide biosynthesis, flagellar synthesis, and the AI-2 quorum-sensing system [ 18 ]. (hindawi.com)
When compared with historic strains, recent isolates showed increased expression of flagellar genes and genes involved in lipopolysaccharide biosynthesis and decreased expression of polysaccharide capsule genes. (cdc.gov)
Taken together, we demonstrate that for the conditions tested PilG and PilH have inverse regulatory effects on flagellum-dependent and pili-dependent motility in Xcc and that this regulatory impact depends on these proteins influences on genes/proteins involved in flagellar biosynthesis and pilus assembly. (biomedcentral.com)

The pilG gene product, required for Pseudomonas aeruginosa pilus production and twitching motility, is homologous to the enteric, single-domain response regulator CheY. (microbiologyresearch.org)
Regulated in parallel to CT are pilus genes, which allow effective colonization of the digestive tract. (kenyon.edu)

The chromosome encodes a complete type VI secretion system and plasmid genes encode type IV secretion proteins. (nottingham.ac.uk)
also called T6SS-1 [23]) provides consistently been proven to be crucial for intercellular pass on and virulence in pets (16 24 T6SS-5 can be necessary for virulence by and (15 25 Around 15 primary genes and a adjustable variety of nonconserved item components encode the T6SS "injectisome" (26 27 Among the core genes are and VgrG (19 20 33 and ADP ribosylation of actin by VgrG1 (34). (healthandwellnesssource.org)
Two such genes in Drosophila encode the enzymes dopa decarboxylase (Ddc) and tyrosine hydroxylase (ple) . (sdbonline.org)
The genomes of Antiqua and Nepal516 are 4.7 Mb and 4.5 Mb and encode 4,138 and 3,956 open reading frames, respectively. (asm.org)

The bacterial flagellar motor (BFM) is an example of an IRM which relies on ion movement through the stator proteins to generate the rotation of the flagella. (bvsalud.org)

Intracellular motility of LI ultimately leads to cell dissemination, which enhances LI's ability to penetrate mucous layers and colonize the intestinal tract. (biomedcentral.com)

Thus, the pyrD gene appears to be essential for several cellular processes involved in AIEC virulence. (rossimicrolab.com)
An emerging theme in the field is how genome organisation in unicellular organisms is affected by cellular environment and growth conditions. (universiteitleiden.nl)
The studies of molecular aspects of genome organisation determined in vitro have yielded models for their in vivo functionality, which urgently require testing in the cellular context. (universiteitleiden.nl)
Serial aerobic passage increased aerotolerance and peroxide stress resistance but motility, growth rate and cellular morphology were unaffected. (nottingham.ac.uk)
Contributing to the interest of the present topic is the fact that modulation of gene activity involves the sensing of intra- and inter-cellular conditions, DNA binding and DNA dynamics, and interaction with the replication/transcription machinery of the cell. (ibecbarcelona.eu)
In addition, it has cellular structures and mechanism that give it motility in the environment it resides in. (kenyon.edu)
Like most bacteria, Xcc uses a variety of extracellular protein structures to interact with their surrounding environment and drives cellular movement. (biomedcentral.com)
These extracellular protein structures called flagella and pili contribute cellular movement in the form of 'swimming' and 'swarming', respectively. (biomedcentral.com)

Eight mutants deficient in twitching motility were isolated. (microbiologyresearch.org)
These mutants were deficient in twitching motility, showing that both the PA1822 and the PA0171 gene are involved in twitching motility. (microbiologyresearch.org)
We have identified a number of genes through nutrient-sensitized genome-wide screen using yeast mutants that are likely involved in the function or formation of the mitochondrial respiratory chain. (tamu.edu)
The main module of NeMeSys is a comprehensive collection of defined meningococcal mutants in strain 8013, consisting of individual mutants in 1,584 non-essential protein-coding genes. (cnrs.fr)

The findings of our previous study suggest that the motility of L. agilis BKN88 enables gut colonization in murine models. (nature.com)

Well-known examples are the eukaryotic and archaeal histones that wrap DNA and the nucleoid-associated proteins in bacteria and archaea that bend and bridge DNA. (universiteitleiden.nl)
It is remodeled actively in eukaryotes by motor enzymes that can move or displace nucleosomes, in addition to remodeling mechanisms more ubiquitous in bacteria and archaea that are based on the interplay between multiple architectural proteins. (universiteitleiden.nl)
Models suggest that in bacteria and archaea there are direct effects of physicochemical factors such as osmolarity, temperature and pH on the action of chromatin proteins, in addition to indirect effects by modulating the expression ratios of different types of chromatin proteins. (universiteitleiden.nl)
Firstly, they are ubiquitous as T4F genes are found in virtually all bacteria and archaea, which underlines an essential ecological role. (cnrs.fr)

We show that, in the AIEC strain LF82, inactivation of the pyrD gene, encoding dihydroorotate dehydrogenase (DHOD), an enzyme of the de novo pyrimidine biosynthetic pathway, completely abolished its ability of to grow in a macrophage environment-mimicking culture medium. (rossimicrolab.com)
A PA0171 mutant and a PA1822 mutant were constructed by gene replacement in the P. aeruginosa PAO1 strain. (microbiologyresearch.org)
She dubbed the hybrid the Rip strain, after Rip Van Winkle, because the gene has awoken from a 700-million-year sleep. (uncommondescent.com)

The bacteria exhibit potent collagen-digesting properties, and genome sequencing has identified novel metalloprotease, collagenase and chitinase enzymes representing potential virulence factors. (biomedcentral.com)

Within the research in Molecular Biology, one important field along the years has been the analyses on how prokaryotes regulate the expression of their genes and what the consequences of these activities are. (ibecbarcelona.eu)

Kinesins are microtubule-dependent motor proteins, some of which moonlight as microtubule polymerases, such as the yeast protein Kip2. (bvsalud.org)

1. The genome-editing tool CRISPR/Cas9 has facilitated easily targeted, effective, high throughput genome editing. (asu.edu)
Our first research stream consists in the high-throughput functional analysis of the N. meningitidis genome using a toolbox named NeMeSys. (cnrs.fr)

In addition to dissecting these mechanistic aspects of gene regulation, we are interested in the roles and physiological consequences of the products of genes regulated by chromatin proteins. (universiteitleiden.nl)
Our data further support the role of ppGpp participating in the coordinated regulation of the expression of bacterial factors required during infection. (ibecbarcelona.eu)
The genes pertaining to cell membranes, such as transmembrane transport, cell wall organization, and regulation of cell shape, were enriched. (biomedcentral.com)
However, despite the numerous studies on bacterial motility in other Gram-negative bacteria, only limited work has been carried out examining the motility regulation in Xcc . (biomedcentral.com)
Genes amplified in B. pertussis include those involved in motility and respiration, whilst in M. tuberuclosis, functions included intracellular growth and regulation of virulence. (cdc.gov)

2016). Dynamic metabolic exchange governs a marine algal-bacterial interaction. (dsmz.de)
6 identified a gene cluster, hbpCAD , encoding the upper metabolic pathway of OPP which involves the transformation of OPP to 2-hydroxypenta-2,4-dienoateand benzoic acid (BA). (nature.com)
The downstream transformation of BA involved a meta -cleavage pathway, although its genetic organization and function was not revealed and the overall network of genes driving the full metabolic pathway of OPP is still not known. (nature.com)
First described to modulate metabolic adaptive responses, ppGpp modulates the expression of genes belonging to very diverse functional categories. (ibecbarcelona.eu)
The undergraduate student(s) will use these prioritized candidate genes to test their requirement for mitochondrial bioenergetics by placing the gene(s) in the metabolic pathway by using biochemical and molecular genetic techniques. (tamu.edu)

Genome analysis of C. concisus strains isolated from saliva samples has identified a bacterial marker that is associated with active Crohn's disease (one major form of IBD). (frontiersin.org)

NCBI accession #NC_008011) exhibits genes responsible for flagellar assembly. (biomedcentral.com)
Rod-shaped cell body of ''C. thermocellum'']] ==Genome structure== The number of nucleotides present in the genome of ''C. thermocellum'' has been discovered and reported to be at 3,843,301 base pairs which makes up 3307 genes [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genome&cmd=Retrieve&dopt=Overview&list_uids=20502]. (kenyon.edu)

The genetic information stored in the DNA of organisms from all three domains of life is folded into a structure that is compact, yet functionally organised in a way that permits genome activity. (universiteitleiden.nl)
Duchenne muscular dystrophy, or DMD, is a genetic condition in which sufferers lack the ability to produce dystrophin - an essential structural protein in muscles. (thenakedscientists.com)
Towards comprehensive understanding of bacterial genetic diversity: large-scale amplifications in Bordetella pertussis and Mycobacterium tuberculosis. (cdc.gov)
Bacterial genetic diversity is often described solely using base-pair changes despite a wide variety of other mutation types likely being major contributors. (cdc.gov)
This reveals the unrecognized and dynamic genetic diversity of B. pertussis and M. tuberculosis, highlighting the need for a more holistic understanding of bacterial genetics. (cdc.gov)

ppGpp is an intracellular sensor that, in response to different types of stress, coordinates the rearrangement of the gene expression pattern of bacteria to promote adaptation and survival to new environmental conditions. (ibecbarcelona.eu)

Co-immunoprecipitation, bacterial two-hybrid and pull-down analyses showed that PilH and PilG were able to interact with district subsets of proteins that potentially account for their regulatory impact. (biomedcentral.com)

We identified 391 essential genes, which are associated with four basic functions (see Figure). (cnrs.fr)

IS element-mediated homologous recombination results in excision or insertion of flanking genome regions and leads to genome reduction and decay ( 14 - 16 ), as well as genome rearrangements ( 17 , 18 ) and large duplications ( 19 ). (cdc.gov)

The experiments also found that oMAP4 can create links between different microtubules and act as a brake to prevent the filaments being moved excessively by motor proteins. (elifesciences.org)

Campylobacteriosis is a major cause of bacterial gastroenteritis in man. (nottingham.ac.uk)

Genome Biol. (dsmz.de)
RNA biol, 2012) and cell motility (Shahapure et al. (universiteitleiden.nl)

Quorum sensing allows a means of cell to cell communication in which a designated sender cell produces quorum sensing molecules that modify gene expression of a designated receiver cell. (asu.edu)

Chromosome 21 gene dosage effects and truncating mutations of the X-chromosomal transcription factor GATA1 synergize to trigger TMD and AMKL in most patients. (embl.de)
Aerotolerance was associated with mutations to genes involved in central and carbohydrate metabolism. (nottingham.ac.uk)

The genome atlases allowed for distinguishing distinct DNA structures and highlighted suspicious high transcription zones. (mdpi.com)
An interactive web-based Pseudomonas aeruginosa genome database: discovery of new genes, pathways and structures. (microbiologyresearch.org)
The Glasner Lab investigates mechanisms of protein evolution in order to understand how the biophysical properties of proteins influence their capacity to evolve new structures and functions. (tamu.edu)

The protein is composed of 279 amino acids. (hindawi.com)

One pathogenic factor of Xcc that is gaining more notoriety in virulence is motility. (biomedcentral.com)

This folded structure including the associated architectural proteins is referred to as chromatin. (universiteitleiden.nl)
Remodeling of chromatin is also mediated by chemical modifications of the DNA, the architectural proteins that shape it or physicochemical cues. (universiteitleiden.nl)
Also, my group is investigating the (architectural) interplay between different types of chromatin proteins in vitro (Laurens et al. (universiteitleiden.nl)
As transcription of many genes and operons responds to environmental changes and as these are often mediated by chromatin proteins, it is expected that 1) the architectural interplay of such proteins and/or 2) their direct response to physicochemical changes determines loop formation and dissolution leading to altered transcription levels. (universiteitleiden.nl)

Furthermore, a previous study indicated that gene order rearrangements associated with IS elements can alter gene expression profiles in B. pertussis ( 20 ). (cdc.gov)

However, the protein-protein interactions between CLas and D. citri are largely unknown. (uibk.ac.at)

A function of Pseudomonas aeruginosa PAO pili: twitching motility. (microbiologyresearch.org)
PilG and PilH antagonistically control flagellum-dependent and pili-dependent motility in the phytopathogen Xanthomonas campestris pv. (biomedcentral.com)
Here we present evidence demonstrating the role of two single domain response regulators PilG and PilH in the antagonistic control of flagellum-dependent (swimming) and pili-dependent (swarming) motility. (biomedcentral.com)
Additionally, flagellum-dependent and pili-dependent motility are essential to Xcc 's ability to attach to host surfaces and to elicit disease. (biomedcentral.com)
Given the importance of flagellum-dependent and pili-dependent motility for Xcc survival and ability to cause disease, it is critical that these systems are effectively regulated and controlled. (biomedcentral.com)

Bacterial biofilms: a common cause of persistent infections. (microbiologyresearch.org)

Independent of these cytological differences, all genomes are shaped by the action of abundant proteins with architectural function. (universiteitleiden.nl)

Just as a modern laptop would run poorly if its processor were replaced with an 1980s-era computer chip, the microbes' modern molecular machinery simply wasn't well suited to the ancient version of the protein. (uncommondescent.com)

The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)

We investigate the activity of these proteins in vitro as well as in vivo using biochemical and state-of-the-art biophysical approaches. (universiteitleiden.nl)
A flavin-dependent monoxygenase (OppA1), one of the most up-regulated proteins in the OPP-growing cells, was isolated via heterologous expression and its catabolic activity was verified in vitro . (nature.com)
Genome instability was further described in vitro using long-read sequencing via the Nanopore platform, which revealed that clonally derived laboratory cultures produced heterogenous populations rapidly. (cdc.gov)

More importantly, we are using and developing new approaches to investigate genome organisation and its coupling to transcription in vivo. (universiteitleiden.nl)
The student will investigate the genes responsible for the Dead phenotype. (tamu.edu)

The partner proteins have undergone their own evolutionary changes over the last 700 million years - they're designed to work with the laptop's modern components, not the outdated chip. (uncommondescent.com)

E264 (35) and Bp340 [1026b Δ(E264 included ((((transposon comprising genes from Bp340 (to -kanamycin resistance gene (39) and a hemagglutinin (HA) tag cloned between the XbaI-SacI sites. (healthandwellnesssource.org)

Genome folding is tightly interconnected with transcription, with genes in certain regions being silenced, while others are highly transcribed. (universiteitleiden.nl)
Instead, the entire genome is tightly packaged in a small region called the nucleoid and transcription takes place between the cytoplasm and nucleoid. (kenyon.edu)

this is the first-time fungal genomes were assembled from ISS metagenomes. (biomedcentral.com)

Our interest lies in understanding how architectural proteins act on DNA and how they regulate transcription. (universiteitleiden.nl)
Our current aim is to obtain an integrated picture of the interplay between spatial genome organization and DNA transactions in general and transcription in specific. (universiteitleiden.nl)

The sigma-54 was found to control genes involved in nitrogen uptake and metabolism including amtB1, glnB-amtB2, ntrX and narK which contribute to the long-term environmental survival of Leptospira spp. (wikipedia.org)
In both Gram-positive and Gram-negative bacteria, 'competence' for DNA uptake requires the coordinated action of rough-ly two dozen genes ( including some with regulatory func-tions ) organized within several operons. (asmblog.org)

in turn an impact on motility and ability to tolerate cell wall stress was observed. (wikipedia.org)
That's because EF-Tu is a hub protein, interacting with 50 or more other proteins as it carries out its function in the cell. (uncommondescent.com)
This proteins established fact for its connect to polar cell spore and development development in Gram-positive bacterias, but little is well known about its function within a predatory development context. (bibf1120.com)
we recognize a canonical ParAB set necessary for predatory cell department and display a BTH relationship between a gene item encoded through the same operon as DivIVA using the canonical Em fun? (bibf1120.com)
o de protein are both portrayed in but aren't necessary for predator cell department. (bibf1120.com)
2015). The partitioning of DNA means that cell department does not take place across nucleoids, as the partitioning of proteins means that each little girl cell gets the prerequisite elements for ideal fitness. (bibf1120.com)
The presence of B cell linear confirmative epitopes of the protein predicted by a bioinformatics tool was validated by western blot analysis using anti-LI mouse hyperimmune serum, which implied that LI0570 induced production of antigen-specific antibodies in vivo. (biomedcentral.com)
This result indicates that the LI0570 protein can trigger an innate immune response followed by a T-cell-related adaptive immune response in an infected host. (biomedcentral.com)
The tools and contexts are diverse, ranging from catalytic RNAs in cell-free systems to bacterial proteins expressed in human cell lines, yet they exhibit an organizing principle: that genes and proteins may be treated as modular units that can be moved from their native organism to a novel one. (asu.edu)
Using transgenic human cell lines, I found that gene-silencing heterochromatin impacts Cas9's ability to bind and cut DNA in a site-specific manner and I investigated ways to improve CRISPR/Cas9 function in heterochromatin. (asu.edu)
Selection of laboratory and/or imaging studies to rule out conditions other than migraine headache is determined by the individual presentation (eg, erythrocyte sedimentation rate and C-reactive protein levels may be appropriate to exclude temporal/giant cell arteritis). (medscape.com)

The new approach, which Kacar presented yesterday at NASA's Astrobiology Science Conference in Chicago, provides a more integrated view of the mechanisms of evolution - for example, how a protein's position in a broader network influences its rate of change or how protein networks evolve as a whole. (uncommondescent.com)

The summer research project in the Gohil lab will focus on discovering evolutionarily conserved proteins that are required for mitochondrial energy metabolism. (tamu.edu)

The twitching-motility defect in the PA1822 mutant was partially complemented by providing the PA1822 gene in trans , and the defect in the PA0171 mutant was fully complemented when PA0171 was provided. (microbiologyresearch.org)
MATERIALS AND METHODS Bacterial strains and mutant building. (healthandwellnesssource.org)
As shown in model micro-organisms, the most valuable toolbox for determining gene function on genome-scale is a comprehensive and archived collection with one mutant in each non-essential gene. (cnrs.fr)

Bacterial strains and plasmids are described in Table 1 . (hindawi.com)

Anatomy5

Organisms3

Diseases1

Chemicals and Drugs22

Analytical, Diagnostic and Therapeutic Techniques and Equipment15

Phenomena and Processes63

Disciplines and Occupations3

Information Science8

Sequence12

Biofilm9

Pathogen5

Chemotaxis3

Species15

Bacterium5

Whole genome2

Pathogens6

Sequences5

Genomic4

Putative4

Biosynthesis4

Pilus2

Encode4

Flagellar motor1

Intestinal tract1

Cellular8

Mutants4

Colonization1

Bacteria and archaea4

Strain3

Virulence factors1

Prokaryotes1

Yeast1

High throughput2

Regulation5

Metabolic5

Strains isolated1

NCBI2

Genetic5

Intracellular1

Analyses1

Essential genes1

Homologous1

Motor proteins1

Gastroenteritis1

Biol2

Quorum1

Mutations2

Structures3

Amino1

Pathogenic1

Chromatin4

Pertussis1

Interactions1

Pili5

Biofilms1

Cytological1

Molecular1

Microbiota1

Vitro3

Investigate2

Undergone1

Transposon1

Tightly2

Fungal1

Transcription2

Uptake2

Cell11

Mechanisms1

Metabolism1

Mutant3

Plasmids1