• Furthermore, small RNA data was obtained by RNA-Seq in C. glutamicum ATCC 13032. (wikipedia.org)
  • There has been much research on AmtR structure, functions, and regulons in the type strain C. glutamicum ATCC 13032, but little research in other C. glutamicum strains. (frontiersin.org)
  • In this study, chromatin immunoprecipitation and massively parallel DNA sequencing (ChIP-seq) was performed to identify the AmtR regulon in C. glutamicum ATCC 14067. (frontiersin.org)
  • Ten peaks were obtained in the C. glutamicum ATCC 14067 genome including two new peaks related to three operons (RS_01910-RS_01915, RS_15995, and RS_16000). (frontiersin.org)
  • The RS_01910, RS_01915, RS_15995, and RS_16000 are not present in the type strain C. glutamicum ATCC 13032. (frontiersin.org)
  • Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
  • The research on transcription factors in C. glutamicum was mainly focused on the type strain C. glutamicum ATCC 13032. (frontiersin.org)
  • RP4-mediated transfer of mobilizable plasmids in intergeneric conjugation of Escherichia coli donors with Corynebacterium glutamicum ATCC 13032 is severely affected by a restriction system in the recipient that can be inactivated by a variety of exogenous stress factors. (uni-bielefeld.de)
  • These results were confirmed by infection assays with the bacteriophage CL31 from Corynebacterium lilium ATCC 15990. (uni-bielefeld.de)
  • The metabolism of this strain has been reconstructed and is available in the form of a genome-scale metabolic model. (wikipedia.org)
  • In this work, using diverse sequence analysis, we evaluated the repertoire of TFs and sigma factors in the community-associated methicillin resistant S. aureus (CA-MRSA) strain USA300-FPR3757. (biomedcentral.com)
  • The Pyrazines occur ubiquitously in nature and can be strain is able to biosynthesize the pyrazines, including 2,3- biosynthesized by several bacteria. (sagepub.com)
  • By using this procedure, clones of the restriction-deficient mutant strain C. glutamicum RM3 harboring a plasmid library of the wild-type chromosome were checked for their restriction properties. (uni-bielefeld.de)
  • It has been reported that the genome-reduced Bacillus subtilis strain MBG874 showed clear advantages for the production of several heterologous enzymes including alkaline cellulase and protease. (biomedcentral.com)
  • By introducing specific gene modifications related to guanosine and thymidine accumulation, respectively, we demonstrated that genome-reduced strains had greatly improved properties compared to the wild-type strain as chassis cells for the production of these two products. (biomedcentral.com)
  • l -threonine production was increased 2.4-fold when the corresponding gene modifications were introduced into E. coli strain MGF-01 that has a 22 % genome reduction [ 12 ]. (biomedcentral.com)
  • To enhance its aromatic tolerance, we recently applied adaptive evolution using phenol as a sole carbon source and characterized a phenol-adapted R. opacus strain (evol40) and the wild-type (WT) strain by whole genome and RNA sequencing. (biomedcentral.com)
  • The genome of the type strain was sequenced as part of the GEBA (Genomic Encyclopedia of Bacteria and Archaea ) project. (dsmz.de)
  • Even though diverse strains of S. aureus have been extensively studied, and subjected to genome sequencing, the function of a large proportion of their genes remains unidentified. (biomedcentral.com)
  • Ethanol production from selected lignocellulosic hydrolysates by genome shuffled strains of Scheffersomyces stipitis. (scielo.br)
  • The region comprising orf1 and orf2 displayed a strikingly low G+C content and was present exclusively in C. glutamicum strains. (uni-bielefeld.de)
  • In this study, we constructed a series of genome-reduced strains by deleting non-essential regions in the chromosome of B. subtilis 168. (biomedcentral.com)
  • These strains with genome reductions ranging in size from 581.9 to 814.4 kb displayed markedly decreased growth rates, sporulation ratios, transformation efficiencies and maintenance coefficients, as well as increased cell yields. (biomedcentral.com)
  • We re-engineered the genome-reduced strains to produce guanosine and thymidine, respectively. (biomedcentral.com)
  • When present in mixtures with glucose, the recombinant C. glutamicum strains co-utilized D-galacturonate with glucose and D-glucuronate with glucose, respectively. (springeropen.com)
  • Devriese LA, Riegel P, Hommez J, Vaneechoutte M, de Baere T, Haesebrouck F. Identification of Corynebacterium glucuronolyticum strains from the urogenital tract of humans and pigs. (dsmz.de)
  • A comparison of the TF repertoire of S. aureus against 1209 sequenced bacterial genomes was carried out allowing us to identify a core set of orthologous TFs for the Staphylococacceae , and also allowing us to assign potential functions to previously uncharacterized TFs. (biomedcentral.com)
  • We have evaluated the orthologous distribution of these elements in other sequenced bacterial genomes using the repertoire of TFs identified in USA300, and identified a core set of regulators for both the Firmicutes phylum, and the Staphylococacceae group. (biomedcentral.com)
  • Bacterial genomes show great diversity in size and composition, and this diversity is the result of millions of years of horizontal gene transfer and other complex evolutionary events [ 5 , 6 ]. (biomedcentral.com)
  • Given the high number of virulence factors present in these bacteria, and the niche-specific role many of them play during different stages of the infectious process, gene expression must be finely tuned in order to efficiently coordinate their expression, and also continue to preserve energy pools. (biomedcentral.com)
  • Keywords Paenibacillus aceti Genome Pyrazine 16S rRNA gene sequences. (sagepub.com)
  • The aroma compound in the Genome size (bp) 5,611,962 supernatant was detected by headspace solid-phase GC content 47.92% microextraction (HS-SPME) coupled with gas chromatogra- Gene total length (bp) 4,731,522 phy-mass spectrometry (GC-MS) (Li et al. (sagepub.com)
  • The disrupted gene, MSMEG_5817, is conserved in all mycobacteria for which genome sequence information is available, and designated Rv0807 in M. tuberculosis. (bvsalud.org)
  • Over all, this study indicates that aceE gene plays a significant role in the mycolic acid synthesis and affects the colony morphology, biofilm formation of M. smegmatis and bacteria invasion of macrophage. (biomedcentral.com)
  • Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2-methylcitrate cycle. (rhea-db.org)
  • Genome sequencing revealed that the Corynebacterium glutamicum genome contained, besides gltA, two additional citrate synthase homologous genes (prpC) located in two different prpDBC gene clusters, which were designated prpD1B1C1 and prpD2B2C2. (rhea-db.org)
  • The objectives were to evaluate the effects of Corynebacterium glutamicum cell mass (CGCM) supplementation, replacing blood plasma in diets, on the gene expressions of biomarkers associated with peptidoglycan and their correlation with intestinal health in nursery pigs. (usda.gov)
  • Corynebacterium glutamicum that expresses exogenous GAD gene, gadB2 or gadB1 , can synthesize GABA from its own produced Glu. (springeropen.com)
  • Finally, gadB2 and mutant gadB1 were co-expressed under the optimal promoter and RBS combination, thus converted Glu into GABA completely and improved GABA production to more than 25 g/L. This study provides useful promoters and RBS sequences for gene expression in C. glutamicum . (springeropen.com)
  • The genome of L14 for the biosynthesis of alkyl-substituted pyrazines have been contains 5,611,962 bp with a GC content of 47.92 mol%, proposed (Beck et al. (sagepub.com)
  • This genome sequence provides a basis the condensation of two amino acids to a cyclic dipeptide, for elucidating the possible mechanism for the biosynthesis and then converted into the pyrazine molecule (Cheng et al. (sagepub.com)
  • Dihydrodipicolinate synthase (DHDPS) is a regulatory enzyme in the lysine biosynthetic pathway and is the first and major rate-limiting enzyme for lysine biosynthesis in plants and bacteria. (canada.ca)
  • 2011 ). Recently, two metabolic routes for de novo biosynthesis of GABA from glucose have been employed in C. glutamicum , both using its own produced Glu as precursor. (springeropen.com)
  • 2022. "Cg1246, a New Player in Mycolic Acid Biosynthesis in Corynebacterium Glutamicum. (paris-saclay.fr)
  • The human pathogen and aetiological agent of tuberculosis, Mycobacterium tuberculosis has a cell wall architecture similar to the non-pathogenic bacterium Corynebacterium glutamicum. (bham.ac.uk)
  • As virulence determinant production is very tightly regulated in S. aureus , a thorough understanding of its regulatory network is necessary to fully comprehend the pathogenic processes of this bacterium. (biomedcentral.com)
  • Foodborne diseases are caused by food contaminated by pathogenic bacteria such as Escherichia coli, Salmonella, Staphylococcus aureus, Listeria monocytogenes, Campylobacter, and Clostridium, a critical threat to human health. (usda.gov)
  • These acidic sugars are naturally consumed by most plant pathogenic bacteria such as Erwinia carotovora , Pseudomonas syringae , and Agrobacterium tumefaciens , but also by E. coli . (springeropen.com)
  • The search for and study of substances that have bactericidal or bacteriostatic properties against human and animal pathogens are also required for the development of new antibiotic therapy or disinfectants for objects and surfaces that have been in close contact with patients and therefore may carry pathogenic bacteria. (encyclopedia.pub)
  • Corynebacterium is a genus of Gram-positive , facultatively anaerobic , non- motile , rod-shaped actinobacteria . (wikidoc.org)
  • Identification and Characterization of Corynaridin, a Novel Linaridin from Corynebacterium lactis . (uni-ulm.de)
  • Genome-assisted Identification, Purification, and Characterization of Bacteriocins. (uni-ulm.de)
  • Neither aldohexuronic acid supported growth of C. glutamicum as sole or combined carbon source, although its genome encodes a putative uronate isomerase sharing 28% identical amino acids with UxaC from Escherichia coli . (springeropen.com)
  • The availability of their genome sequences has enabled the utilisation of C. glutamicum as a model for the identification and study of essential mycobacterial genes involved in the synthesis of cell wall components such as lipomannan (LM), lipoarabinomannan (LAM) and arabinogalactan (AG). (bham.ac.uk)
  • Heterologous expression of the genes for both uptake and catabolism of D-galacturonic acid and D-glucuronic acid was required to enable growth of C. glutamicum with either aldohexuronic acid as the sole carbon source. (springeropen.com)
  • 2020. "Genome-Wide Identification of Novel Genes Involved in Corynebacteriales Cell Envelope Biogenesis Using Corynebacterium Glutamicum as a Model. (paris-saclay.fr)
  • Leverage our advanced machine learning tools, ultra high throughput automation, and proprietary bacterial and fungal production hosts-including Escherichia coli , Bacillus subtilis , Pichia pastoris , Saccharomyces cerevisiae , Aspergillus niger , Corynebacterium glutamicum , Yarrowia lipolytica , and many more model and nonmodel host organisms-to search through the vastness of biological design space to improve your products today. (ginkgobioworks.com)
  • While originally identified in a screen for organisms secreting L-glutamate, mutants of C. glutamicum have also been identified that produce various other amino acids. (wikipedia.org)
  • This plasmid, termed pRES806, is able to complement the restriction-deficient phenotype of different C. glutamicum mutants. (uni-bielefeld.de)
  • Microorganisms of various types exist in all three domains of life (the Bacteria, Archaea, and Eukarya), and they are by far the most abundant life forms on Earth. (researchgate.net)
  • Corynebacterium glutamicum is a Gram-positive and generally regarded as safe (GRAS) microorganism with less endotoxicity. (frontiersin.org)
  • Corynebacterium glutamicum is generally regarded as safe and is widely used for the industrial production of Glu, l -lysine (Lys) and other amino acids (Leuchtenberger et al. (springeropen.com)
  • Interference with the function of MSMEG_5817 may provide a novel therapeutic approach for control of mycobacterial pathogens by assisting the host immune system in clearance of persistent intracellular bacteria. (bvsalud.org)
  • The essentiality of DprE1 in M. smegmatis, combined with its conservation in all sequenced mycobacterial genomes, suggests that decaprenylphosphoryl arabinose synthesis is essential in all mycobacteria. (bvsalud.org)
  • BACKGROUND: Bacteria of the suborder Corynebacterineae include significant human pathogens such as Mycobacterium tuberculosis and M. leprae. (bvsalud.org)
  • BACKGROUND: The unique cell wall of bacteria of the suborder Corynebacterineae is essential for the growth and survival of significant human pathogens including Mycobacterium tuberculosis and Mycobacterium leprae. (bvsalud.org)
  • Mycolic acids are essential components of the cell walls of bacteria belonging to the suborder Corynebacterineae, including the important human pathogens Mycobacterium tuberculosis and Mycobacterium leprae. (bvsalud.org)
  • Recently, some specific Gram-positive bacteria, including Mycobacterium tuberculosis and Corynebacterium glutamicum , were found to possess a novel cell wall structure consisting of an arabinogalactan layer covalently linked to the peptidoglycan layer, and a special outer membrane consisting of mycolic acids (mycomembrane) [ 19 ]. (nature.com)
  • Some nondiphtheria species of Corynebacterium produce disease in specific animal species, and some of these are also human pathogens . (wikidoc.org)
  • As a novel antibacterial agent against foodborne pathogens, endolysins are peptidoglycan hydrolases encoded by bacteriophages that lyse bacterial cells by targeting their cell wall, notably in Gram-positive bacteria due to their naturally exposed peptidoglycan layer. (usda.gov)
  • Different bacteriocins act against Gram-positive or Gram-negative bacteria, fungal pathogens and amoeba cells. (encyclopedia.pub)
  • This organism is a well-studied soil bacterium of considerable importance in biotechnology, in particular for the fermentative production of L-amino acids for food and fodder industry. (up.ac.za)
  • Upon pRErm46 acquisition, Tn RErm46 stabilizes itself in R. equi by transposing to the host genome, including the conjugative virulence plasmid pVAPA. (cdc.gov)
  • In the genus Corynebacterium , AmtR is a key component of the nitrogen regulatory system, and it belongs to the TetR family of transcription regulators. (frontiersin.org)
  • Members of this genus are gener- Leucine Threonine dehydratase ally Gram-positive, rod-shaped, endospore-forming, aerobic or facultatively anaerobic bacteria. (sagepub.com)
  • In this study, we have analysed several uncharacterised open reading frames, which encode for putative glycosyltransferases from M. tuberculosis and deleted their respective orthologues in C. glutamicum. (bham.ac.uk)
  • High-efficiency production of the antimicrobial peptide pediocin PA-1 in metabolically engineered Corynebacterium glutamicum using a microaerobic process at acidic pH and elevated levels of bivalent calcium ions. (uni-ulm.de)
  • Corynebacterium glutamicum , which is used in industrial amino acid production at a million-ton scale, has been engineered towards utilization of alternative carbon sources. (springeropen.com)
  • The amino acid sequences of IntI integrases have been used as a basis for dividing integrons into 'classes', with those carrying intI1 defined as 'class 1', intI2 as 'class 2', intI3 as 'class 3', etc. intI1 , intI2 and intI3 were first identified in association with mobile genetic elements and intI4 and others with chromosomal integrons. (biomedcentral.com)
  • Since its discovery, C. glutamicum has become an indispensable microorganism for the biotechnological industry [ 9 ]. (springeropen.com)
  • Utilization of the hexuronic acids D-galacturonic acid (abundant in pectin-rich waste streams such as peels and pulps) and D-glucuronic acid (a component of the side-chains of plant xylans) for growth and production with C. glutamicum has not yet been studied. (springeropen.com)
  • In this study a rapid test procedure based on intergeneric conjugal plasmid transfer that permitted the distinction between restriction-negative and restriction-positive C. glutamicum clones was developed. (uni-bielefeld.de)
  • C. glutamicum is currently used commercially to produce glutamate and other amino acids (L-lysine) and compounds. (up.ac.za)
  • analyses gradually elucidated the perfected genome-scale metabolic map of C. glutamicum , expanding the scope of its application. (frontiersin.org)
  • Genome analyses revealed PcaQ-like DNA-binding sites encoded upstream of ABC transport systems in other members of the α-proteobacteria, and thus it appears likely that these systems are involved in the uptake of protocatechuate. (microbiologyresearch.org)
  • Our data takes the lead in demonstrate that the threonine transporter Rv3737 is required for in vitro growth and survival of bacteria inside macrophages. (biomedcentral.com)
  • Therefore, optimization of promoters and RBS sequences has been applied for protein expression in C. glutamicum . (springeropen.com)
  • Due to its industrial importance, several clones of C. glutamicum have been sequenced by both industry and academic groups. (wikipedia.org)
  • Soil bacterium with industrial uses. (up.ac.za)
  • For example, aph promoter combined with different RBS sequences were used for expression of reporter proteins in C. glutamicum (Zhang et al. (springeropen.com)
  • Genome-scale genetic manipulation has a variety of effects on the physiological characteristics and cell metabolism of B. subtilis . (biomedcentral.com)
  • Corynebacterium diphtheriae , the cause of diphtheria in humans. (wikidoc.org)
  • They may be found as members of the normal microflora of humans, where these bacteria find a suitable niche in virtually every anatomic site. (up.ac.za)
  • R. opacus is a Gram-positive actinomycete bacterium that can accumulate triacylglycerols (TAGs), a biodiesel precursor, up to ~ 78% of its cell dry weight when grown on sugars [ 25 ]. (biomedcentral.com)