• For adenovirus-positive aerosol samples, 500 μL of sample was inoculated into adenocarcinomic human alveolar basal epithelial (A549) cells (ATCCR CCL 185™) with Dulbecco's Modified Eagle Medium (DMEM) 2% (v/v) Fetal Bovine Serum (FBS), and incubated at 37°C. After 72 hours, inoculated shell vials were observed for cytopathic effect (CPE) daily for ten days. (datexis.com)
  • Longitudinal whole genome analysis of pre and post drug treatment Mycobacterium tuberculosis isolates reveals progressive steps to drug resistance. (cdc.gov)
  • fluid were collected for antimicrobial susceptibility testing and After the increase in summer 2021, no evidence of higher lev- whole-genome sequencing at CDC's Streptococcus reference els of intensive care unit (ICU) admission, mortality, genetic laboratory to identify microbiological features shared among relatedness of isolates from different patients, or increased cases. (cdc.gov)
  • With sequencing technologies becoming cheaper and easier to use, more groups are able to obtain whole genome sequences of viruses of public health and scientific importance. (biomedcentral.com)
  • SARS-CoV-2 whole-genome sequencing was performed using a tiled-amplicon sequencing approach. (biomedcentral.com)
  • Available Streptococcus specimens isolated from a brain abscess, epidural empyema, subdural empyema, blood, or cerebrospinal fluid were collected for antimicrobial susceptibility testing and whole-genome sequencing at CDC's Streptococcus reference laboratory to identify microbiological features shared among cases. (medscape.com)
  • In conclusion, this study established a protocol for enterovirus and HPeV virus identification that combines conventional and molecular methods and would be beneficial for HPeV diagnosis. (omicsdi.org)
  • Additionally, we obtained the complete or near-complete genome of bovine rhinitis B, enterovirus E1, bovine viral diarrhea virus (sub-genotypes 1a and 1c) and bovine respiratory syncytial virus, and partial sequences of other viruses. (biomedcentral.com)
  • The 400 known viruses are classified in several ways: by genome core (RNA or DNA), host (animals, plants, or bacteria), method of reproduction (such as retrovirus), mode of transmission (such as enterovirus), and disease produced (such as hepatitis virus). (unboundmedicine.com)
  • Enterovirus genotyping was performed by partial sequencing of the VP1 region of the enterovirus genome. (who.int)
  • Enterovirus C105 (EV-C105) is a member of the human enterovirus group C species and was first identified in the Democratic Republic of Congo in late 2010 in a faecal sample collected from a fatal acute flaccid paralysis patient during a poliomyelitis outbreak. (who.int)
  • 2 Due to a lack of sequence data this virus was initially identified as Enterovirus 109 (EV-109). (who.int)
  • 3 In 2012, the virus was assigned as EV-C105 because subsequent sequencing showed that it had only 74.7% nucleotide and 82.5% amino acid identity to EV-109, thus satisfying the criteria for assignment of a new enterovirus serotype. (who.int)
  • EV-D68-positive specimens were further screened for parainfluenza virus 1-4, coronaviruses (NL63, OC43, HKU1, and 229E), and bocavirus by using 2-step real-time PCRs (oligonucleotide sequences can be provided on request). (cdc.gov)
  • The human coronaviruses (HCoVs) HCoV-NL63 and HCoV-HKU1 are two recently discovered coronaviruses that circulate widely and are associated with acute respiratory infections (ARI). (hindawi.com)
  • Before the outbreak of severe acute respiratory syndrome caused by the SARS coronavirus in 2003, human coronaviruses (HCoVs) had not been considered harmful respiratory pathogens. (hindawi.com)
  • HCoV-NL63, a member of the group I coronaviruses, was first detected in a child with bronchiolitis in The Netherlands in 2004 [ 1 ]. (hindawi.com)
  • The observed virucidal activity of Echinaforce® was not restricted to common cold coronaviruses, as both SARS-CoV-1 and MERS-CoVs were inactivated at comparable concentrations. (biomedcentral.com)
  • Coronaviruses (CoVs) are enveloped, positive-sense, single-stranded RNA viruses with a large genome, typically 26-32 kb in length. (biomedcentral.com)
  • However, the more virulent coronaviruses, Middle East respiratory syndrome (MERS)-CoV and SARS-CoV-1 have animal reservoirs with proposed origins in bats [ 7 ] and can cause severe pneumonias with longer incubation periods and often fatal outcome [ 8 ]. (biomedcentral.com)
  • 6 Compared with known coronaviruses that can infect humans, the SARS-CoV-2 structure has certain differences, so it is defined as the seventh coronavirus. (medsci.org)
  • 9 Coronavirus is characterized by spikes sticking out from the surface, which in part have the same characteristics as SARS-CoV, and other four Human coronaviruses HCoV-NL63 (human coronavirus NL63), HCoV-229E (human coronavirus 229E), HCoV-OC43 (human coronavirus OC43), and HCoV-HKU1 (human coronavirus HKU1), which only cause mild respiratory diseases. (medsci.org)
  • Frey S, Essbauer S, Zöller G, Klempa B, Weidmann M , Dobler G, Pfeffer M (2013) Complete Genome Sequence of Tick-Borne Encephalitis Virus isolated from a Yellow-necked Mouse ( Apodemus flavicollis ) in Austria Genome Announcements 1(4). (mweidmann.com)
  • The complete genome sequence of J vi- them than ever ( 1 ). (cdc.gov)
  • With the advent of reverse genetics, it has been found that the most efficient human parainfluenza viruses (in terms of replication and transcription) have a genome nucleotide total that is divisible by the number 6. (wikipedia.org)
  • Viral replication is initiated only after successful entry into a cell by attachment and fusion between the virus and the host cell lipid membrane. (wikipedia.org)
  • Towards the end of the process, (after the formation of the viral proteins) the replication of the viral genome occurs. (wikipedia.org)
  • Numerous studies have shown that replication of RNA viruses is involved in many specific RNA-RNA, RNA-protein, and protein-protein interactions. (biomedcentral.com)
  • In contrast disease launch from untransformed human being fibroblasts or main human being hepatocytes was found to be reduced by about three log PF-04620110 methods in a time course experiment which enables the cumulation of kinetic variations of the unique phases of viral replication such as primary PF-04620110 target cell illness target cell replication and progeny disease particle release. (siamtech.net)
  • an electron microscope study of wild human rotavirus replication in cell cultures. (liverpool.ac.uk)
  • There is an urgent need for information on virus replication, immunity and infectivity in specific sites of the body. (nature.com)
  • Here we report a detailed virological analysis of nine cases of COVID-19 that provides proof of active virus replication in tissues of the upper respiratory tract. (nature.com)
  • We consistently detected sequence-distinct virus populations in throat and lung samples from one patient, proving independent replication. (nature.com)
  • The confirmation of active virus replication in the upper respiratory tract has implications for the containment of COVID-19. (nature.com)
  • Here we provide a detailed virological analysis of nine cases, providing proof of active virus replication in upper respiratory tract tissues. (medrxiv.org)
  • Active virus replication in the upper respiratory tract puts prospects of COVID-19 containment in perspective. (medrxiv.org)
  • Non-structural proteins are involved in the transcription and replication of the virus. (medsci.org)
  • In HHV-1 and HHV-2 oral infections, viral replication within the oral epithelium may cause lysis of epithelial cells, with vesicle formation. (medscape.com)
  • PREDAC-H3: a user-friendly platform for antigenic surveillance of human influenza a(H3N2) virus based on hemagglutinin sequences. (cdc.gov)
  • Three Genome Sequences of Legionella pneumophila subsp. (cdc.gov)
  • Genomic sequences were generated with an Illumina Miseq [ 3 ] instrument, and single-nucleotide polymorphisms (SNPs) were identified for core genomes employing kSNP3.0 with k-mer size of 19. (medscape.com)
  • Both multiplex one-step RT-PCR and RT-Bst PCR were used to compare their performances for detection of virus sequences. (westminster.ac.uk)
  • RT-Bst and RT-Bst PCR can be used as alternative approaches to reverse transcription and one-step RT-PCR, respectively, for sequence-independent amplification of RNA virus sequences and a larger scale analysis of this new diagnostic approach is warranted. (westminster.ac.uk)
  • 6 , 8 , 10 The genome and subgenome of the new coronavirus contain at least 6 open reading frames (ORFs), which generally have 5' leader and 3' end sequences. (medsci.org)
  • Deduced amino acid sequences at the fusion protein cleavage site of Newcastle disease viruses showing variation in antigenicity and pathogenicity. (microbiologyresearch.org)
  • Therefore we investigated the virucidal and antiviral potential of Echinacea purpurea (Echinaforce®) against human coronavirus (HCoV) 229E, highly pathogenic MERS- and SARS-CoVs, as well as the newly identified SARS-CoV-2, in vitro. (biomedcentral.com)
  • Pre-treatment of cell lines, however, did not inhibit infection with HCoV-229E and post-infection treatment had only a marginal effect on virus propagation at 50 μg/ml. (biomedcentral.com)
  • Detection of Sindbis and Inkoo Virus RNA in Genetically Typed Mosquito Larvae Sampled in Northern Sweden. (cdc.gov)
  • rotavirus antibody determination by the neutralization test and electron microscopic detection of viruses in children with acute gastroenteritis]. (liverpool.ac.uk)
  • epidemic acute gastroenteritis in newborn infants with the immunoelectronoptic detection of virus]. (liverpool.ac.uk)
  • Importantly, not only did these studies identify previously unrecognised viral species, but they also consistently observed an abundance of viruses not part of the regular veterinary diagnostic laboratory detection range, including influenza D virus (IDV), bovine rhinitis viruses A and B (BRAV and BRBV), parvoviruses and bovine herpesviruses other than bovine alphaherpesvirus-1 (BoHV-1). (biomedcentral.com)
  • An internal control based on the detection of human endogenous nucleic acid sequence (RNase P) present in human genome has been employed in order to check RNA extraction, PCR inhibition and sampling or application errors. (anatoliageneworks.com)
  • We employed a novel method, RT-Bst, to enrich the concentration of cDNA for subsequent multiplex PCR detection of selected RNA viruses. (westminster.ac.uk)
  • Virus detection. (westminster.ac.uk)
  • A roadmap to translating the microbiome (special issue) Begum R. Genome Med, Apr 28, 2016. (cdc.gov)
  • SNPs) were identified for core genomes employing kSNP3.0 fluctuations observed since 2016. (cdc.gov)
  • With CHA, CDC examined hospitalizations at 40 tertiary referral children's hospitals across the United States that consistently reported data to PHIS during January 1, 2016-May 31, 2022 (the most recent data available when the analysis was performed). (medscape.com)
  • State and federal public health laboratories have built capacity such that they now sequence more than 6000 influenza virus genomes and more than 5000 enteropathogenic bacterial genomes each year [ 11 ]. (biomedcentral.com)
  • Similarly, respiratory syncytial virus (RSV) and cytomegalovirus (CMV) can be detected in the presence of other known bacterial pathogens. (medscape.com)
  • Streptococcus pyogenes is a major human-specific bacterial pathogen that causes a wide array of manifestations ranging from mild localized infections to life-threatening invasive infections. (nih.gov)
  • [ 1 ] Pediatric bacterial brain abscesses, epidural empyemas, and subdural empyemas, rare complications of respiratory infections and sinusitis, are often caused by Streptococcus species but might also be polymicrobial or caused by other genera, such as Staphylococcus . (medscape.com)
  • RÉSUMÉ La flambée d'une infection par le nouveau coronavirus en Arabie saoudite en 2012 a principalement affecté des hommes et des résidents urbains. (who.int)
  • Novel coronavirus (nCoV) was first isolated in June 2012 from the respiratory secretions of a businessman in the Bisha area of Saudi Arabia, who subsequently died of pneumonia and renal failure [1]. (who.int)
  • Coronavirus disease 2019 (COVID-19) is an acute infection of the respiratory tract that emerged in late 2019 1 , 2 . (nature.com)
  • Bosphore SARS-CoV-2/Flu/RSV Panel Kit detects and discriminates 2019-nCoV (which is later renamed officially as severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by WHO), RSV A&B and influenza A&B in human respiratory samples including nasopharyngeal, oropharyngeal swabs and saliva. (anatoliageneworks.com)
  • Through collaboration with the Children's Hospital on or after June 1, 2021, irrespective of etiology. (cdc.gov)
  • Because the program launched in 2021, the Traveler-based Genomic Surveillance (TGS) program has offered an important window into how the COVID-19 virus is circulating, particularly since extra persons are counting on at-home checks that dont require them to report outcomes. (lowehomeimprovements.com)
  • In CDC's national call for cases, a case was defined as the diagnosis of brain abscess, epidural empyema, or subdural empyema in a person aged ≤18 years without a previous neurosurgical procedure or history of head trauma, hospitalized on or after June 1, 2021, irrespective of etiology. (medscape.com)
  • MMWR Surveill Summ 70(No. SS-5):1-32, 2021. (msdmanuals.com)
  • Viruses 2021, 13, 1642. (iame-research.center)
  • Evolution of a Dominant Natural Isolate of Escherichia coli in the Human Gut over the Course of a Year Suggests a Neutral Evolution with Reduced Effective Population Size. (iame-research.center)
  • To evaluate the antiviral potential of the extract, we pre-treated virus particles and cells and evaluated remaining infectivity by limited dilution. (biomedcentral.com)
  • The Structure of Human Parechovirus 1 Reveals an Association of the RNA Genome with the Capsid. (omicsdi.org)
  • We found that interactions among pentamers in the HPeV-1 capsid are mediated by the N termini of VP0s, which correspond to the capsid protein VP4 and the N-terminal part of the capsid protein VP2 of other picornaviruses. (omicsdi.org)
  • In order to facilitate delivery of the virus genome into the cytoplasm, the N termini of VP0s have to be released from contacts between pentamers and exposed at the particle surface, resulting in capsid disruption. (omicsdi.org)
  • A hydrophobic pocket, which can be targeted by capsid-binding antiviral compounds in many other picornaviruses, is not present in HPeV-1. (omicsdi.org)
  • Therefore, putative small molecules that could disrupt HPeV RNA-capsid protein interactions could be developed into antiviral inhibitors.Human parechoviruses (HPeVs) are pathogens that cause diseases ranging from respiratory and gastrointestinal disorders to encephalitis. (omicsdi.org)
  • Furthermore, we found that the interactions of the HPeV-1 genome with the capsid resulted in a partial icosahedral ordering of the genome. (omicsdi.org)
  • Viruses that assemble in this way have to balance the formation of nuclei with the growth of the capsid. (sciencedaily.com)
  • Viral architecture is very complex, but every virus contains at least a genome and a capsid. (unboundmedicine.com)
  • Viruses are also responsible for the common cold, childhood exanthems (such as chickenpox, measles, rubella), latent infections (such as herpes simplex), some cancers or lymphomas (such as Epstein-Barr virus), and diseases of all organ systems. (unboundmedicine.com)
  • Although transmission of the virus to health-care workers was suspected in Jordan's April 2012 outbreak, similar clusters have not been found in Saudi Arabia's hospitals, nor have additional cases been identified through retrospective tracing of exposed health-care workers. (who.int)
  • Due to the sequence of events and full genome sequence data, one person was considered the index case for this outbreak, directly infecting 67 to 83% of the teammates. (biomedcentral.com)
  • The outbreak of SARS renewed interest in this virus family and resulted in the identification of two additional HCoVs, HCoV-NL63 and HCoV-HKU1. (hindawi.com)
  • Human parainfl uenza recurrent pneumococcal meningitis pneumococci, although cerebrospinal virus 4 outbreak and the role of diagnostic developed, caused by a vaccine fl uid culture was negative. (cdc.gov)
  • Specimens were collected from patients with influenza-like illness/acute respiratory infection by physicians participating in national outpatient acute respiratory infection sentinel surveillance in Germany ( 1 ). (cdc.gov)
  • Human rhinoviruses (HRVs) are a highly prevalent cause of acute respiratory infection in children. (plos.org)
  • Parechovirus A (Human parechovirus, HPeV) causes symptoms ranging from severe neonatal infection to mild gastrointestinal and respiratory disease. (omicsdi.org)
  • Among these viruses, we could highlight those belonging to the Flaviviridae and Togaviridae families, which may cause clinical manifestations ranging from asymptomatic infection, acute febrile to disease viral haemorrhagic fever and encephalitis. (fapesp.br)
  • H7N9 highly pathogenic avian influenza virus (HPAIV) infection in a human was first reported in 2017. (bvsalud.org)
  • Concurrent COVID-19 diagnosis was defined as past year and the possible contributing role of SARS-CoV-2 having International Classification of Diseases, Tenth Revision infection ( 1 ). (cdc.gov)
  • Nearly 100% of children in the USA are infected with the virus by 2 to 3 years of age, several hundred infants may die directly from the infection, while the deaths of an additional several thousand may be attributed to RSV-related complications ( Nair et al, 2010 ). (intechopen.com)
  • The ability of a pathogenic virus to lie dormant within a cell (latent infection). (lookformedical.com)
  • In this study, we screened for the presence of HCoV-NL63 and HCoV-HKU1 in children with acute respiratory infection admitted to the Beijing Children's Hospital in an effort to gain a better understanding of the seasonality, epidemiology and genetic diversity of these viruses in a city with a population of more than 22 million. (hindawi.com)
  • the finding of extra rna fragments with respect to the regular 11 genome segments suggests the possibility of simultaneous or sequential infection by more than one electropherotype in a single diarrhea event or occurrence of modification in the length of the rna segments during an infection. (liverpool.ac.uk)
  • virus-specific immunity in neonatal and adult mouse rotavirus infection. (liverpool.ac.uk)
  • mouse rotavirus (epizootic diarrhea of infant mice) was used as a model to study the role of virus-specific immunity in infection and diarrheal disease. (liverpool.ac.uk)
  • Aging is characterized by a progressive decline in the capacity of the immune system to fight influenza virus infection and to respond to vaccination. (biomedcentral.com)
  • Aging is usually associated with reduced influenza virus-specific and influenza vaccine-specific antibody responses but some elderly individuals with higher pre-exposure antibody titers, due to a previous infection or vaccination, have less probability to get infected. (biomedcentral.com)
  • HHV-2, also known as HSV-2, causes genital herpes and occasionally causes oral disease that is clinically similar to that of HHV-1 infection. (medscape.com)
  • HHV-3, also known as varicella-zoster virus (VZV), causes the primary infection chickenpox and the secondary reactivation disease herpes zoster. (medscape.com)
  • HHV-4, also known as Epstein-Barr virus (EBV), causes the primary infection infectious mononucleosis , and it is implicated in various diseases, such as African Burkitt lymphoma , other immunoproliferative disorders, and nasopharyngeal carcinoma. (medscape.com)
  • [ 5 , 6 ] In a localized primary infection, the virus penetrates the mucosal epithelium and invades the cells of the basal layer, where the viral DNA inserts into the host DNA. (medscape.com)
  • The pathogenicity of HPIVs is mutually dependent on the viruses having the correct accessory proteins that are able to elicit anti-interferon properties. (wikipedia.org)
  • A virus with reduced pathogenicity as a result of treatment or repeated passage through hosts. (unboundmedicine.com)
  • For instance, virulent field strain Herts/33 with the F cleavage site 112 RRQRRF 117 had an intracerebral pathogenicity index of 1·88 compared with 1·28 for strain NDFLtag, which has the same cleavage site. (microbiologyresearch.org)
  • In order to extract maximal scientific and public health value out of these data, sharing of assembled consensus genomes and raw sequence data is critical. (biomedcentral.com)
  • Virions are approximately 150-250 nm in size and contain negative sense RNA with a genome encompassing about 15,000 nucleotides. (wikipedia.org)
  • HPIV genomes are about 15,000 nucleotides in length and encode six key structural proteins. (wikipedia.org)
  • It contains a single-stranded positive RNA genome of 10,976 nucleotides (nts) in length that encodes a single large open reading frame (ORF) flanked by a 5'-untranslated region (5'-UTR, 95 nts) and a 3'-untranslated region (3'-UTR, 585 nts). (biomedcentral.com)
  • The virus Manoharan and his team studied, which infects E. coli bacteria, is about 30 nanometers in diameter and has one piece of RNA, with about 3600 nucleotides, and 180 identical proteins. (sciencedaily.com)
  • The predicted amino acid identity of the VP0-VP3-VP1 region of the three viruses was 74.6%-74.8%, 73.4%-73.6%, and 97.0%-97.1% when compared to HPeV-1, -2, and -3 prototype strains, respectively. (omicsdi.org)
  • monoclonal antibodies were derived against the sa11 simian, nic bovine, and wa human rotavirus strains and characterized by enzyme-linked immunosorbent assay, plaque neutralization, and hemagglutination inhibition. (liverpool.ac.uk)
  • Virulence of Newcastle disease virus (NDV) is mainly determined by the amino acid sequence surrounding the fusion (F) protein cleavage site, since host proteases that cleave the F protein of virulent strains are present in more tissues than those that cleave the F protein of non-virulent strains. (microbiologyresearch.org)
  • Japanese encephalitis virus (JEV) is a mosquito-borne flavivirus that causes acute encephalitis in humans, with a high fatality rate of 20 to 50% [ 1 ]. (biomedcentral.com)
  • mixed human rotavirus electropherotypes were detected in stool samples from patients with acute gastroenteritis in santiago, chile. (liverpool.ac.uk)
  • [1] Ineffective treatment of S. pyogenes infections can result in the postinfectious sequela acute rheumatic fever and post-streptococcal glomerulonephritis. (nih.gov)
  • Until the emergence of Severe Acute Respiratory Syndrome (SARS)-CoV-1 in 2002, HCoVs were thought to mainly be responsible for the common cold. (biomedcentral.com)
  • Many foresee a world in which nearly every infectious disease genome is sequenced and archived in a publicly searchable database [ 9 , 10 ]. (biomedcentral.com)
  • Virus shedding is an important means of vertical transmission (INFECTIOUS DISEASE TRANSMISSION, VERTICAL). (lookformedical.com)
  • Nuclear antigens encoded by VIRAL GENES found in HUMAN HERPESVIRUS 4. (lookformedical.com)
  • Two real-time RT-PCR assays targeting conserved regions of the N and 1ab genes of both viruses were developed and used in parallel. (hindawi.com)
  • AAVs are nonenveloped single-stranded DNA viruses used in gene therapy to insert copies of missing genes into host cells. (unboundmedicine.com)
  • Dilcher M, Sall A, Hufert FT, Weidmann M (2013) Clarifying Bunyamwera virus riddles of the past Virus Genes 47(1):160-3. (mweidmann.com)
  • GenBank currently has automatic prokaryotic and eukaryotic genome annotation pipelines but has no viral annotation pipeline beyond influenza virus. (biomedcentral.com)
  • Parechoviruses are human pathogens that cause diseases ranging from gastrointestinal disorders to encephalitis. (omicsdi.org)
  • It is transmitted to humans by the mosquito or tick bite and is implicated in some cases of encephalitis. (unboundmedicine.com)
  • Proteins found in any species of virus. (lookformedical.com)
  • To enable oncolytic applications of SeV we here engineered a set of novel recombinant vectors by a two-step approach: (i) intro of an ubiquitously identified cleavage-motive into SeV fusion protein right now enabling continuous distributing in human being cells and (ii) serious attenuation of these rSeV from the knockout of viral immune modulating accessory proteins. (siamtech.net)
  • How those proteins manage to form that structure is the central question in virus assembly. (sciencedaily.com)
  • The researchers also noticed that the viruses tended to misassemble more often when there were more proteins flowing over the substrate. (sciencedaily.com)
  • Vaccination may be with attenuated or inactivated virus, or with viral proteins produced by genetic engineering techniques. (justia.com)
  • Structural proteins are all encoded by the 3' terminus of the viral genome. (medsci.org)
  • That observation might give us some insights into how to derail the assembly of pathogenic viruses," said Manoharan. (sciencedaily.com)
  • Members of the human herpesvirus (HHV) and human papillomavirus (HPV) families cause the most common primary viral infections of the oral cavity. (medscape.com)
  • The mechanism by which latent viruses, such as genetically transmitted tumor viruses ( PROVIRUSES ) or PROPHAGES of lysogenic bacteria, are induced to replicate and then released as infectious viruses. (lookformedical.com)
  • While some viruses and bacteria have been widely studied and are known to be an important component of the respiratory complex, novel, emerging or unrecognised pathogens are not easily detected because they are not routinely considered in the differential diagnosis or laboratory diagnostic assays are not available. (biomedcentral.com)
  • The BioFire-FilmArray-Respiratory Panel 2.1 test was used for 742 nasopharyngeal swabs [identification of 19 viruses (including SARS-CoV-2) and four bacteria], while the BioFire-FilmArray-Pneumonia Panel was used [identification of 18 bacteria and nine viruses] (BioMerieux, Marcy l'Etoile, France) for 107 tracheal aspirates. (bvsalud.org)
  • Mutator genomes decay, despite sustained fitness gains, in a long-term experiment with bacteria. (iame-research.center)
  • HCoV-HKU1, a group II virus, was detected in an adult with chronic pulmonary disease in Hong Kong in 2005 [ 2 ]. (hindawi.com)
  • Double or triple infections with HRV-C and respiratory syncytial virus and/or bocavirus were diagnosed in 33.3% of the HRV-infected patients, but no correlation with severity of clinical outcome was observed. (plos.org)
  • The viruses isolated from nasopharyngeal aspirates grew slowly on tertiary monkey kidney cells and were successfully passaged on Vero cells. (omicsdi.org)
  • Unrelated to the reason for presenting to the hospital, cough and wheeze were noted on the second day of his admission and a nasopharyngeal swab for respiratory virus testing was collected. (who.int)
  • Sequence-distinct virus populations were consistently detected in throat- and lung samples of one same patient. (medrxiv.org)
  • SignificanceHepatitis C virus chronically infects approximately 1% of the world's population, making an effective vaccine for hepatitis C virus a major unmet public health need. (bvsalud.org)
  • Our technique gives the first window into how viruses assemble and reveals the kinetics and pathways in quantitative detail. (sciencedaily.com)
  • As a consequence IFN-sensitive viruses preferentially replicate in malignancy cells while normal cell types and cells are able to launch a powerful counterattack [9]. (siamtech.net)
  • ABBR: AAV A genus in the parvovirus family whose members cannot replicate without the presence of another virus. (unboundmedicine.com)
  • Significant viral diseases in cattle are infectious bovine rhinotracheitis, parainfluenza, bovine viral diarrhea, rabies, and foot and mouth disease. (justia.com)
  • Unfortunately, the use of antibiotics across different livestock systems, as well as its use in humans, has resulted in a widespread emergence of antimicrobial resistance (AMR), an increasing global public health threat. (biomedcentral.com)
  • Human rhinoviruses and enteroviruses in influenza-like illness in Latin America. (medscape.com)
  • Viruses transmitted by arthropods (arboviruses) are responsible for emerging and reemerging infectious diseases in different areas of the world, especially in tropical and subtropical regions. (fapesp.br)
  • SARS-CoV-2 is detected by two regions of the virus (Orf1ab and N gene nCoV specific) from a single channel FAM. (anatoliageneworks.com)
  • Subsequently, genomic transcription occurs using the viruses own 'viral RNA-dependent RNA polymerase' (L protein). (wikipedia.org)
  • Genomic characterization and phylogenetic analysis of Zika virus circulating in the Americas. (cdc.gov)
  • Nucleic acid was extracted using QIAamp MinElute Virus Spin kits (Qiagen, Mississauga, Ontario, Canada) according to the manufacturer's instructions. (hindawi.com)
  • This transmission electron micrograph (TEM) revealed some of the nucleocapsid morphologic features displayed by the human parainfluenza virus Type-4a (HPIV-4), a member of the Paramyxoviridae family. (eol.org)
  • A third serotype of human parechovirus (HPeV) has been recently isolated from stool specimens of a young Japanese child with transient paralysis. (omicsdi.org)
  • genome double-stranded rnas isolated from purified human reovirus (serotype 3) and rotavirus (wa strain) were modified at the 3' termini by addition of oligo(c) approximately 15 with t4 rna ligase. (liverpool.ac.uk)
  • months later, she was brought to the org/10.1128/JVI.79.16.10690-10700.2005 background rate ( 1 , 2 ). (cdc.gov)
  • A probable new human picornavirus associated with respiratory diseases. (medscape.com)
  • While the severity of bovine respiratory disease (BRD) varies, it can account for up to 70% of the mortality observed in intensive beef production systems (i.e., feedlot cattle) [ 1 ]. (biomedcentral.com)
  • 1 In addition, untyped enteroviruses are referred to ESR for further identification. (who.int)
  • A transgenic mouse with enhanced viral resistance transmissible to its offspring is prepared by introduction of a gene encoding a human interferon having antiviral activity into a host mouse. (justia.com)
  • Orlando: Academic Press, Inc., 1986, Vol. 119(C). The interferons do not act directly against the virus, rather, they stimulate the antiviral response of the immune system. (justia.com)
  • Additionally a first-in-man software of recombinant Sendai disease has been reported very recently like a vector for gene delivery of human being FGF-2 to treat peripheral arterial disease and was shown to be safe and well tolerated [17]. (siamtech.net)
  • Preferably, the human beta interferon gene is introduced into the cells of a mouse. (justia.com)
  • Ten thousand copies of a human leukocyte IFN gene were microinjected into each zygote. (justia.com)
  • In Brazil, the virus that stand out are the Dengue virus (DENV), Chikungunya virus (CHIKV) and Zika virus (ZIKV), the last two first identified in 2014 and 2015 respectively, causing severe Public Health problems, especially in the Northeast region of Brazil. (fapesp.br)