Genome bacterium plasmids. Medical search. Frequent questions

is useful as the sequences did not match any known plasmid sequence deposited in public databases. (frontiersin.org)
Entrez is NCBI's primary text search and retrieval system that integrates the PubMed database of biomedical literature with 38 other literature and molecular databases including DNA and protein sequence, structure, gene, genome, genetic variation and gene expression. (nih.gov)
Sequence-directed genetic interference pathways control gene expression and preserve genome integrity in all kingdoms of life. (rockefeller.edu)
Upon infection, CRISPR arrays can acquire new spacer units that match the sequence of the infecting phage or plasmid. (rockefeller.edu)
The complete genetic package, or genome, of S. islandicus contains a set of core genes that are shared among all members of this group, with some minor differences in the sequence of nucleotides that spell out individual genes. (sciencedaily.com)
Genome sequence of Pseudomonas citronellolis SJTE-3, an estrogen- and polycyclic aromatic hydrocarbon-degrading bacterium. (pacb.com)
Here, we report its whole-genome sequence, containing one circular chromosome and one circular plasmid. (pacb.com)
Since scientists were able to sequence the genome, they now have information of how diseases caused by this pathogenic bacteria are developed and also the evolutionary history of the bacteria. (kenyon.edu)
Sandwalk: The 20th anniversary of the human genome sequence: 2. (blogspot.com)
It's been 20 years since the first drafts of the human genome sequence were published. (blogspot.com)
The IHGP sequence covered about 82% of the genome and it contained about 250,000 gaps and millions of sequencing errors. (blogspot.com)
International Human Genome Sequencing Consortium (2004) Finishing the euchromatic sequence of the human genome. (blogspot.com)
The amount of actual nucleotide sequence in the latest version of the reference genome ( GRCh38.p13 ) is 3,110,748,599 bp and the estimated total size is 3,272,116,950 bp based on estimating the size of the remaining gaps. (blogspot.com)
It's now possible to complete the human genome reference sequence by sequencing at least one individual but I'm not sure that the effort and the expense are worth it. (blogspot.com)
So even if we could perfectly sequence two genomes, their total lengths would not be the same. (blogspot.com)
He has already shown that genomes can be built from scratch, by taking the gene sequence of the bacterium Mycoplasma genitalium and constructing it in the lab. (nextbigfuture.com)
however, as evidenced by genome sequence analysis, the organism lacks common enzymes required for the de novo synthesis of precursors of PGN, which rationalizes its MurNAc auxotrophy. (karger.com)
Complete genome sequence of geobacter sp. (nebraska.edu)
Dive into the research topics of 'Complete genome sequence of geobacter sp. (nebraska.edu)
A short, fossilized femur from a 38-year-old Neandertal, which sat untouched in a museum in Zagreb, Croatia, could lead to the first full genome sequence of Homo sapiens 's closest relative and help scientists understand what is special about humans, say teams that published analyses of two partial sequences of Neandertal DNA in this week's issues of Science and Nature . (scientificamerican.com)
Comparative sequence analysis of pPATH pathogenicity plasmids in Pantoea agglomerans gall-forming bacteria. (tau.ac.il)
Complete genome sequence of an Israeli isolate of Xanthomonas hortorum pv. (tau.ac.il)
It isn't clear whether the presence of the SV40 sequence in the plasmid DNA of mRNA vaccines was identified by Pfizer when the company applied for approval from the FDA. (zerohedge.com)
Empirically derived sequence similarity thresholds to study the genomic epidemiology of plasmids shared among healthcare-associated bacterial pathogens. (cdc.gov)
If the sequence result was of low quality, the amplicon was cloned into a plasmid vector using a TOPO TA cloning kit (Invitrogen, Carlsbad, California) and then sequenced using the primers provided with the kit. (who.int)

The work package focusing on molecular studies generated new evidence about the changes effected by antibiotic therapy on commensal organisms or opportunistic pathogens in the oropharyngeal, nasal and gastro-intestinal flora and study AMR mechanisms and the dissemination of successful clones of fluoroquinolone-resistant, carbapenem-resistant or extended-spectrum beta-lactamase harboring Gram-negative bacteria, MRSA and fluoroquinolone-resistant viridans streptococci. (europa.eu)

Whitaker's team found that the variable genome in individual strains of S. islandicus is evolving at a rapid rate, with high levels of variation even between two or three individuals in the same location. (sciencedaily.com)
To evaluate this, we exposed wild type and mutant strains to pH=4 and determinateviable bacteria after treatment. (conicet.gov.ar)
Lysogenic phages have evolved to insert their DNA into the genomes of specific strains of bacteria. (igem.org)
Shigella strains harboring PMQR genes were identified earlier this year following whole genome sequencing of isolates from a multistate outbreak of multidrug-resistant Shigella flexneri infections predominantly affecting adult men, many of whom identify as men who have sex with men, according to epidemiologic data collected by CDC's Shigella program as part of outbreak response. (cdc.gov)
Comparing the assembled genome with reference strains facilitates many different inferences, such as pathogen identification, high-resolution strain typing, and prediction of important phenotypic characteristics (e.g., virulence, antimicrobial resistance). (cdc.gov)
Well-curated and up-to-date reference databases are crucially important because microbial pathogens evolve rapidly and bacteria can exchange plasmids—often encoding virulence and antimicrobial resistance traits—across strains and species. (cdc.gov)

See how the University of Washington used HiFi sequencing to uncover a key finding about ALS and the human genome. (pacb.com)
These first drafts from the International Human Genome Project (IHGP) and Celera were far from complete. (blogspot.com)
We've known for many decades that the correct size of the human genome is close to 3,200,000 kb or 3.2 Gb. (blogspot.com)
Morton, N.E. (1991) Parameters of the human genome. (blogspot.com)
The glossary features nearly 250 terms explained in an easy-to-understand way by leading scientists and professionals at the National Human Genome Research Institute. (genome.gov)
The pieces are then sequenced directly, and researchers reassemble them by mapping them to similar sections in the human genome. (scientificamerican.com)
Each of these figures is a drop in the bucket considering the human genome is 3.2 billion base pairs long. (scientificamerican.com)

However, phages mutate in an effort to bypass these defenses: a mutation in the region of the phage genome corresponding to, say, a blue spacer means that the phage can attack and escape the defense of bacteria with blue spacers (fuzzy green circle), but not bacteria with green, orange or pink spacers (red X inside a circle). (elifesciences.org)
The first step is to choose restriction enzymes using webcutter 2.0 that appear only once , closest to the region of the phage genome that is going to be modified (Fig. 1). (igem.org)

The mcr-1 gene was first reported in 2015 in food, animal, and patient isolates from China ( 1 ) and is notable for being the first plasmid-mediated colistin resistance mechanism to be identified. (cdc.gov)
Comparisons among the genomes revealed a high level of diversity among the isolates that cause disease. (usda.gov)
The current model predicts that phytopathogenic isolates require a cluster of three loci present on a linear plasmid, with the fas operon central to virulence. (usda.gov)
We sequenced and compared the genomes of 20 isolates of Rhodococcus to gain insights into the mechanisms and evolution of virulence in these bacteria. (usda.gov)
Preliminary data suggest that all Shigella isolates with ciprofloxacin MICs in this range for which results are available harbor at least one quinolone resistance gene known to confer reduced susceptibility in enteric bacteria. (cdc.gov)
Rising fluoroquinolone MIC values among Shigella isolates may be related to the emergence of plasmid-mediated quinolone resistance (PMQR) genes in Shigella species in the United States. (cdc.gov)

To address the gap of knowledge on this, the complete sequences of eight plasmids from Pandoraea spp. (frontiersin.org)
The information on plasmid sequences in Pandoraea spp. (frontiersin.org)
Within CRISPR sites, the repeats are separated by short spacer sequences that match phage or plasmid genomes and specify the targets of interference. (rockefeller.edu)
MTase methylates specific DNA sequences (recognition sites) in the host genome. (biomedcentral.com)
Right here, we display that the CRISPR-MAD7 system can be utilized for genome enhancing and acknowledges T-rich PAM sequences (YTTN) in crops. (trancheproject.org)
These sequences are cloned into the bacterium Escherichia coli using USER technology, where they are amplified and then assembled into a single plasmid. (diwou.com)
The genome has many insertion sequences and many G-C base pair differences, which means frequent recombination. (kenyon.edu)
Highly repetitive regions are unstable in plasmid/cosmids/BACs so their length and sequences couldn't be accurately determined. (blogspot.com)
Rubin's used a metagenomic approach, incorporating fragments of Neandertal DNA into loops called plasmids, amplifying them in bacteria and then using known sequences of human DNA to isolate strands for sequencing. (scientificamerican.com)
Comparing the similar sequences of Neandertal and human DNA, Rubin's group determined that the two genomes are at least 99.5 percent identical. (scientificamerican.com)
This will require secondary DNA sequences including the phage genome integration site , some garbage DNA (flanked by essential restriction enzyme cut sites) and the low-copy number BioBrick plasmid . (igem.org)
In fact, as reported by Drancourt et al (2004), genotyping performed on bacteria derived from the remains of plague victims of the first two epidemics revealed sequences similar to that of Orientalis. (medscape.com)

CRISPR-Cas systems enable bacteria to acquire immunity against their viruses by capturing snippets of their DNA and using RNA-guided nucleases that cleave the viral DNA. (rockefeller.edu)
Geneticists can now create millions of new viruses and bacteria in a matter of minutes in the laboratory. (i-sis.org.uk)
In the experiment on retroviral genomes, 6 mouse leukaemia viruses (MLV) were recombined in a single round, giving a library of 5 million replicating recombinant viruses. (i-sis.org.uk)
As these researchers have amply demonstrated, recombination is the major route to generating new viruses and bacteria. (i-sis.org.uk)
Some of the recombinant viruses and bacteria could well be lethal. (i-sis.org.uk)
Are the recombinant viruses and bacteria, or their DNA, strictly contained within the laboratory? (i-sis.org.uk)
Archaea, like bacteria, can transfer genes to one another, but they also obtain new genes from free-floating genetic elements, called plasmids, or from viruses that infect the cells and insert their own genes into the archaeal DNA. (sciencedaily.com)
What did vary in the genomes of S. islandicus could be traced back to plasmids and viruses, Whitaker said. (sciencedaily.com)
Most of the genes that are coming and going, at least on Sulfolobus, seem to be on viruses and plasmids," Whitaker said. (sciencedaily.com)
The viruses and plasmids that had lent their genes to Sulfolobus in one site were different from those found in another. (sciencedaily.com)
Many of the genes have been acquired from other bacteria and viruses. (kenyon.edu)
iPHoP: A Matchmaker for Phages and their Hosts Building on existing virus-host prediction approaches, a new tool combines and evaluates multiple predictions to reliably match viruses with their archaea and bacteria hosts. (doe.gov)
Many bacteria use a system known as CRISPR-Cas to defend themselves against infection by viruses called phages. (elifesciences.org)
Bacteria versus viruses called phages. (elifesciences.org)
This chapter describes basic principles of ecology and evolution for waterborne viruses, bacteria, and protozoa (and yeasts and molds to a lesser extent) of public health concern as an aid to better understand how selective forces may alter one's ability to assess the microbial quality of water. (nationalacademies.org)
In recombinant DNA technology, DNA molecules are manipulated using naturally occurring enzymes derived mainly from bacteria and viruses. (pressbooks.pub)
The examinations were performed at the Centre d'Infectiologie Charles Mérieux (CICM) in Bamako with the screening of the genome of viruses responsible for Dengue, Rift Valley fever, and Zika using the real-time RT-PCR technique. (bvsalud.org)

Additionally, genes conferring virulence and antibiotic resistance were identified amongst the plasmids. (frontiersin.org)
Hence, the spread of virulence factors and antibiotic resistance properties caused by plasmids is possible, leading to a threat in public health. (frontiersin.org)
Synergistic interaction between the type III secretion system of the endophytic bacterium Pantoea agglomerans DAPP‐PG 734 and the virulence of the causal agent of olive knot Pseudomonas savastanoi pv. (zhaw.ch)
These comparisons also showed that a specific cytokinin plant hormone is associated with virulence of these bacteria. (usda.gov)
The virulence of this bacterium results from the 32 Y pestis chromosomal genes and two Y pestis -specific plasmids, constituting the only new genetic material acquired since its evolution from its predecessor. (medscape.com)
The key to the organism's virulence is the phenomenon of "blockage," which aids the transmission of bacteria by fleas. (medscape.com)

AMR, through acquired genes located on transposons or conjugative plasmids, is the horizontal transmission of genes required for a given bacteria to withstand antibiotics. (mdpi.com)
During this period, Erik developed a fascination for mobile genetic elements, in particular the role played by plasmids and transposons in acquiring and transporting antibiotic resistance genes. (asm.org)
however, genome integrations with these Cas-directed transposons require a cargo plasmid carrying the desired DNA payload flanked by directed repeat transposon arms. (biorxiv.org)

In bacteria and archaea, CRISPRs-clustered, regularly interspaced, short palindromic repeats-constitutes a recently discovered genetic interference pathway that protects cells from phages and conjugative plasmids. (rockefeller.edu)
Marraffini ultimately hopes to answer fundamental questions about how CRISPR-Cas systems destroy their targets, how the genetic memory is generated, and how CRISPR-Cas immunity affects the evolution of bacteria and archaea. (rockefeller.edu)
Acquirement and loss of an R-M system is a routine event in the evolution of most bacteria and archaea. (biomedcentral.com)
Once lumped together with bacteria, archaea are now classified as a separate domain of life. (sciencedaily.com)
Archaea are really different from bacteria - as different from bacteria as we are," said University of Illinois microbiology professor Rachel Whitaker, who led the study. (sciencedaily.com)

Following the initial remarkable success of antibiotics, the emergence and spread of human pathogenic bacteria resistant to antibiotics has become a major phenomenon in the past fifty years. (europa.eu)
The emergence and spread of human pathogenic bacteria resistant to antibiotics has become a major problem in the past fifty years. (europa.eu)
These data provide the first genomic comparison in this group of plant pathogenic bacteria and lay the groundwork needed to develop diagnostic tools needed by the nursery industry. (usda.gov)

To develop new and more effective indicators of waterborne pathogens it is important to better understand how both evolution and ecology interact with the genomes and natural history of waterborne pathogens and their indicators, if different from themselves. (nationalacademies.org)
Some bacteria in the genus Rhodococcus cause leafy galls, shoot proliferation, and other growth deformities on many plants and are economically important pathogens of herbaceous perennials grown by the ornamental nursery industry. (usda.gov)
To analyse the possibility that novel plasmid-encoded quinolone resistance determinants (Qnr) can emerge and disseminate among bacterial pathogens, we searched the presence of those elements in nearly 1000 bacterial genomes and metagenomes. (us.es)

Tipically, overexpresion of DGC in heterologous or homologous bacteria augments cdi-GMP intracellular concentration and enhanced biofilm formation. (conicet.gov.ar)
Because ET-A is chromosome borne and ET-B is plasmid borne, multiple intracellular copies of ET-B may exist, which would lead to higher ET-B production. (medscape.com)
Anaplasma phagocytophilum is a gram-negative obligate intracellular bacterium long recognized as a veterinary agent 1 and more recently as a human infection. (who.int)

This system protects the bacterial cell by taking a short length of DNA from the phage and inserting this 'spacer' into its own genome. (elifesciences.org)
Bacteria with these spacers survive infections and pass their spacers on to their progeny, creating a population that is resistant to the phage. (elifesciences.org)
It seems that when bacteria are immobilized, individual cells within the colony must acquire more spacers to resist infection by the mutated phage ( Figure 1 , right). (elifesciences.org)
Lastly, lysogenic phage genomes are too large to be manipulated using normal BioBrick plasmids. (igem.org)
The objectives of our phage standard include negating the issues of genome size, exploiting phage characteristics for use as a vector, and developing a BioBrick compatible standard applicable to all lysogenic phages. (igem.org)
For our project, these insertion vectors will be low-copy BioBrick plasmids containing the integration site of a given phage, flanked by chosen restriction sites. (igem.org)
The phage standard describes the process of adding a given Biobrick part, which we will call source DNA into the genome of a lysogenic phage, referred to as host DNA . (igem.org)
Then transform the plasmid into the host bacterial strain and expose the bacteria to the phage. (igem.org)
This will allow the phage to infect the bacterial cells as well as the integration site on the low-copy BioBrick plasmid. (igem.org)
Current state of the low copy BioBrick plasmid containing the full phage genome. (igem.org)
Cut and ligate it all back together and viola, a modified phage genome is at your disposal. (igem.org)

BioSystems currently contains biological pathways from the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the EcoCyc ( Escherichia coli K-12 MG1655) subset of the BioCyc databases and is designed to accommodate other data in the future. (nih.gov)
In Escherichia coli and related bacteria, we believe that both plasmids and the bacterial chromosome use site-specific recombination to convert multimers to monomers. (gla.ac.uk)

Deinococcus geothermalis has a genome that contains 2.47 Mbp with 2,335 protein coding genes. (wikipedia.org)
The genome contains a total of 3,843 genes and 3,608 protein-coding genes, including genes supporting iron and nitrogen biogeochemical cycling. (nebraska.edu)

Bacterial genomes are composed of two types of replicons: chromosomes and plasmids. (pasteur.fr)
By transplanting the DNA of the first bacteria, Mycoplasma mycoides, into yeast, whose genetics are easier to manipulate, they were able to modify the bacterial chromosomes in two important ways. (nextbigfuture.com)
Results We have found a number of novel potential qnr genes in the chromosomes of aquatic bacteria and in metagenomes from marine organisms. (us.es)

Avoidance of palindromic recognition sites of Type II restriction-modification (R-M) systems was shown for many R-M systems in dozens of prokaryotic genomes. (biomedcentral.com)
We have studied all known recognition sites in thousands of prokaryotic genomes and found factors that influence their avoidance. (biomedcentral.com)
The avoidance of orthodox Type II R-M system recognition sites in prokaryotic genomes is a widespread phenomenon. (biomedcentral.com)
There are now more than 8,000 fully sequenced and annotated prokaryotic genomes available. (ku.dk)

The clinical isolate S. epidermidis RP62a harbors a CRISPR spacer that matches the nickase gene ( nes ) that is present in nearly all staphylococcal conjugative plasmids and prevents their spread. (rockefeller.edu)

In addition to the circular chromosome, ''C. thermocellum'' also has a plasmid which is an extrachromosomal genetic element that is not essential in affecting its lifestyle. (kenyon.edu)
Unlike the circular chromosome which is necessary for viability, the loss of the plasmid will not affect the bacteria's ability to reproduce. (kenyon.edu)

Having the genome sequenced also means that they are able to determine other species that are related to yersinia pestis which can prevent future outbreaks. (kenyon.edu)
Plasmids can be transferred between bacteria, potentially spreading the resistance gene to other bacterial species. (cdc.gov)
Notable exceptions include various species of Vibrio and Legionella bacteria and protozoan parasites such as the free-living amoebae Naegleria and Acanthamoeba . (nationalacademies.org)
Description and significance== Like most species from the Clostridium genus, ''C. thermocellum'' is a bacteria that has a rod-like shape for its cell body. (kenyon.edu)

While in yeast, the genome was altered using yeast genetic systems, and then transplanted to produce a new strain of M. mycoides. (nextbigfuture.com)
The genetic material was inserted into the bacterial cell in the form of a plasmid: a relatively short DNA molecule that remains separate from the bacteria's "natural" genome. (innovationtoronto.com)

These plasmids along with a pathogenicity island called HPI, create a protein that causes the pathogenicity of the organism. (kenyon.edu)

Instead, the entire genome is tightly packaged in a small region called the nucleoid and transcription takes place between the cytoplasm and nucleoid. (kenyon.edu)
Because the entire genome of a laboratory strain of H influenzae has been sequenced, additional insights into its molecular mechanisms of pathogenesis will undoubtedly be gained in the near future. (medscape.com)

An analysis of closely related bacteria shows that such avoidance can be a trace of lost R-M systems. (biomedcentral.com)

Marraffini investigates the molecular mechanisms that make CRISPR immunity possible, and also explores genome editing and other potential applications for CRISPR-Cas systems. (rockefeller.edu)
The RepliChroms project aims at decrypting plasmid adaptation mechanisms in their bacterial host. (pasteur.fr)
It is therefore not surprising to find that circular genomes have evolved mechanisms to ensure that multimers can be effectively converted to monomers. (gla.ac.uk)

The response of bacteria to the threat posed by phages depends on their local environment. (elifesciences.org)
Further experiments showed that compared to bacteria grown in liquid culture, bacterial cells in the resistant colonies had often acquired multiple spacers and were able to fight off phages with other mutations ( Figure 1 , right). (elifesciences.org)
This suggests that priming enables immobile bacteria to survive mutated phages that have escaped detection by allowing the bacteria to create multiple spacers. (elifesciences.org)

The organism was completely isolated and sequenced at the DOE Joint Genome Institute. (kenyon.edu)

Plasmid stabilization/stability protein-encoding genes were observed in some plasmids but were not established for participating in plasmid segregation. (frontiersin.org)
The identification of genes involved in replication, segregation, toxin-antitoxin systems and conjugation, would aid the design of drugs to prevent the survival or transmission of plasmids carrying pathogenic properties. (frontiersin.org)

The recombinants are cloned into a vector and introduced into bacteria for rapid screening. (i-sis.org.uk)

Additionally, there are protein repair enzymes that the bacterium can use to combat oxidative stress, as well as up-regulating catalase and superoxide dismutase. (wikipedia.org)
When the transplanted bacteria in their previous effort failed to function, Venter's team realized that the restriction enzymes might be interfering. (nextbigfuture.com)

Conjugation genes were identified portraying the conjugation ability amongst Pandoraea plasmids. (frontiersin.org)

Evolutionary change requires informational increases in the genomes of organisms. (skeptoid.com)
The first premise is not strictly true: evolution is defined as the adaptation of a population of organisms to its natural environment, and this does not necessarily require the information of the genome to increase. (skeptoid.com)
But, do point mutations cause informational increases in the genomes of organisms, like creationists say they don't? (skeptoid.com)

Antimicrobial Resistance (AMR) is rampant among bacteria that cause healthcare- and community-acquired infections, driving up costs and increasing the difficulty of therapeutic management. (europa.eu)

Although microbial genomes are generally smaller and less complex than human genomes, long-read sequencing technologies (such as single-molecule real-time (SMRT) sequencing, Pacific Biosciences, Menlo Park, CA) are useful for constructing complete, highly accurate genomes and sorting out plasmids, repeats, and other complex regions. (cdc.gov)

The US public health system is integrating pathogen genome sequencing into infectious disease surveillance with support from the Advanced Molecular Detection (AMD) program established by Congress at the Centers for Disease Control and Prevention (CDC) in 2014. (cdc.gov)
Population-level data on pathogen genomes in turn supports the development of more precise and efficient clinical diagnostics. (cdc.gov)
Three studies have shown that this bacterium emerged from the gut pathogen Yersinia pseudotuberculosis shortly after the first epidemic. (medscape.com)

However other regulators systems are present in B. pertussis and needs further research.In the present work we focused on the role of the second messenger c-di-GMP, a key signaling molecule that regulates many importantphysiological processes in bacteria. (conicet.gov.ar)

Whole-genome sequencing reveals widespread presence of Staphylococcus capitis NRCS-A clone in neonatal units across the United Kingdom. (cdc.gov)

Resistance to acidic stress is a phenotyperegulated by c-di-GMP in other bacteria. (conicet.gov.ar)
In 2016, this work led to the first identification of the mobile colistin resistance gene, mcr , in the U.S. Thereafter, Erik developed and published the first model describing the birth and demise of the composite transposon carrying mcr , showing how this gene could be stabilized within a host bacterium through transposon decay. (asm.org)
Functional studies of the Stenotrophomonas maltophilia Smqnr gene show that plasmid-encoded SmQnr confers quinolone resistance upon its expression in a heterologous host. (us.es)
Conclusion Altogether, the data presented in our work support the notion that predictive studies on antibiotic resistance are feasible, using currently available information on bacterial genomes and with the aid of bioinformatic and functional tools. (us.es)
Plasmid-mediated resistance genes are of particular concern because of their ability to spread between bacteria and their ability to promote chromosomal mutations conferring quinolone resistance, potentially resulting in rapid spread of fluoroquinolone resistance within or between populations of bacteria. (cdc.gov)

Often, they are instead the product of the metabolism of yeast and bacteria and are modified and released in wine during the fermentation processes. (frontiersin.org)
This plasmid is inserted into the yeast cell Saccharomyces cerevisieae genome where it produces the receptors. (diwou.com)
We recently reported the chemical synthesis, assembly, and cloning of a bacterial genome in yeast. (nextbigfuture.com)
To produce a synthetic cell, the genome must be transferred from yeast to a receptive cytoplasm. (nextbigfuture.com)
We cloned a Mycoplasma mycoides genome as a yeast centromeric plasmid, and then transplanted it into Mycoplama capricolum to produce a viable M. mycoides cell. (nextbigfuture.com)

Yersinia pestis is a rod shaped gram-negative bacteria that can also have a spherical shape. (kenyon.edu)
It is very important to have the genome sequenced for Y. pestis because this organism is capable of causing very fatal diseases. (kenyon.edu)
The disease is caused by a coccobacillus-shaped, gram negative bacterium referred to as Yersinia pestis . (medscape.com)
The disease is caused by the plague bacillus, rod-shaped bacteria referred to as Yersinia pestis . (medscape.com)

R-M systems are widely spread among prokaryotes: they have been found or predicted with computational methods in the vast majority of bacterial and archaeal genomes [ 13 ]. (biomedcentral.com)

Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)

Extrachromosomal DNA provides great impact in the evolution of bacteria in adapting to their surroundings. (frontiersin.org)
Plasmids are one of the primary sources for extrachromosomal DNA in bacteria and are capable of self-transmission. (frontiersin.org)

However, it still remained unclear what drives bacteria to acquire multiple spacers. (elifesciences.org)

In particular, Deniococcus geothermalis biofilms on printing equipment can help other bacteria form biofilms on top of the existing one, referred to as a secondary biofilm bacterium. (wikipedia.org)

A plasmid is a strand of circular DNA that is common to bacteria and specific parasites. (zerohedge.com)

The BioProject database is a searcheable collection of complete and incomplete (in-progress) large-scale molecular projects including genome sequencing and assembly, transcriptome, metagenomic, annotation, expression and mapping projects. (nih.gov)

Larger pieces of DNA such as entire plasmids, viral and bacterial genomes can also be recombined. (i-sis.org.uk)

Bacillus thuringiensis is a motile, rod-shaped, gram-positive bacterium that is widely distributed in nature, especially in soil and pest-rich environments. (justia.com)
kurstaki HD73 has been cloned into Bacillus subtilis using an integrative plasmid and amplified (Calogero et al. (justia.com)

Tracing the Evolution of Shiitake Mushrooms Understanding Lentinula genomes and their evolution could provide strategies for converting plant waste into sugars for biofuel production. (doe.gov)

This cloning pre-requisite significantly hinders the modularity and streamlining capabilities of Cas-directed transposon systems, diminishing their utility for genome engineering. (biorxiv.org)
Here, we present a system that can robustly integrate a linear DNA payload into the genome of E. coli by employing a Type I-F CRISPR-associated transposon from Vibrio cholerae . (biorxiv.org)

This report summarizes T. forsythia 's strategies to survive in the oral habitat by means of PGN salvage pathways, including recovery of exogenous MurNAc and PGN-derived fragments but also polymeric PGN, which are all derived from cohabiting bacteria either via cell wall turnover or decay of cells. (karger.com)
Pfizer and Moderna COVID-19 mRNA vaccines "are contaminated with plasmid DNA fragments which have not been removed during the current manufacturing processes," the doctor wrote. (zerohedge.com)

Replication genes were not identified in some plasmids, a situation that has led to the possibility of host interaction involvement. (frontiersin.org)
This further leads to the hypothesis of host-plasmid interaction. (frontiersin.org)
The sites of orthodox R-M systems that are encoded in host genomes for a long time are avoided more often (up to 100 % in certain cohorts) than the sites of recently acquired ones. (biomedcentral.com)
A special feature of these bacteria, despite its relatively small genome, is the ability to adapt in different host environments. (aaem.pl)
When the bacteria is in a host, it is nonmotile (incapable of self-propelled movement), but when isolated it is motile. (kenyon.edu)
These factors are important for adhesion and injection of proteins into the host cell, invasion of the bacteria and binding of iron from red blood cells. (kenyon.edu)
Next, find a cut site that is absent in (i) the host DNA, (ii) the BioBrick plasmids and (iii) the source DNA. (igem.org)
This plasmid is the sub host DNA. (igem.org)
In an Aug. 8, 2019, patent filed by Moderna regarding RNA vaccines, the company states that the direct injection of naked plasmid DNA into a living host comes with "potential problems, including the possibility of insertional mutagenesis, which could lead to the activation of oncogenes or the inhibition of tumor suppressor genes. (zerohedge.com)

Yersinia is named in honor of Alexander Yersin, who successfully isolated the bacteria in 1894 during the pandemic that began in China in the 1860s. (medscape.com)

Anatomy9

Organisms44

Diseases4

Chemicals and Drugs45

Analytical, Diagnostic and Therapeutic Techniques and Equipment32

Phenomena and Processes121

Disciplines and Occupations7

Technology, Industry, Agriculture2

Information Science10

Health Care10

Sequence25

Harboring Gram-negative1

Strains6

Human genome7

Region of the phage genome2

Isolates6

Sequences12

Viruses17

Virulence6

Transposons3

Archaea5

Pathogenic bacteria3

Pathogens3

Intracellular3

Phage11

Escherichia2

Protein codin2

Chromosomes3

Prokaryotic genomes4

Conjugative plasmids1

Circular chromosome2

Species4

Genetic2

Pathogenicity1

Entire genome2

Closely related bacteria1

Mechanisms3

Phages3

Joint Genome In1

Segregation2

Vector1

Enzymes2

Genes were identified1

Organisms3

Antimicrobial Resistance1

Repeats1

Pathogen3

Signaling molecule1

Widespread1

Resistance5

Yeast5

Pestis4

Archaeal1

Genomics1

Extrachromosomal2

Acquire1

Biofilm1

Parasites1

Annotation1

Viral1

Bacillus2

Evolution1

Transposon2

Fragments2

Host9

Yersinia1