• The draft whole-genome assemblies of the E. mallotivora isolates are composed of 36-64 contigs with N50 value ranging from 285 to 332 kbp and cover 96.2-100% of the estimated genome size. (qld.gov.au)
  • This study provides the first draft whole-genome sequences of Philippine isolates of E. mallotivora, thus expanding the genomic knowledge for this species in comparison with other members of the genus Erwinia. (qld.gov.au)
  • The efficacy of pSelAct-KO was demonstrated in C. michiganensis and confirmed using whole genome sequencing. (biorxiv.org)
  • Considering the Potential Application of Whole Genome Sequencing to Gonorrhea Prevention and Control. (cdc.gov)
  • Increasingly applied to identify mutations conferring antimicrobial resistance (AMR), disease outbreaks, and pathways of disease spread, whole genome sequencing (WGS)the process of determining the complete DNA sequence of an organisms genome at a single timehas emerged as a powerful tool for public health. (cdc.gov)
  • The positive clones employing the largest halo size were sequenced and a gene encoding a putative lipase was found. (unina.it)
  • We showed the putative T3SS structural genes to be transcriptionally active, and that expression of predicted effector proteins was toxic to yeast in an established functional screen. (nih.gov)
  • We also determined the sequence of the 31-kb SH1 genome and positively identified genes for 11 structural proteins, with putative identification of three additional proteins. (edu.au)
  • Secreted proteins of E. mallotivora were also predicted and characterized to unravel putative genes involved in plant-pathogen interactions. (qld.gov.au)
  • Sequence comparison showed that TnAV-CP is most homologous to the putative capsid proteins from AVs infecting noctuid insects, less homologous to that of Diadromus pulchellus ascovirus 4a (DpAV-4a), further supporting the distinction of two subgroups within the family Ascoviridae. (psu.edu)
  • Phylogenetic analysis using the putative capsid protein suggested that AVs were closely related to members of Iridoviridae, which corroborated the result based on DNA polymerase δ sequences. (psu.edu)
  • Identification of predicted pathogenesis and symbiosis determinants such as the Type III Secretion System (T3SS) in the genomes of "free-living" bacteria suggests that these microbes participate in uncharacterized interactions with eukaryotes. (nih.gov)
  • They both share certain structural similarites that are not seen in bacteria. (bartleby.com)
  • The three-domain tree, containing Eukarya, Archea, and Bacteria, soon became too simplistic due to the realization that some bacteria possessed the ability to exchange genetic information by horizontal gene transfer (Koonin and Wolf, 2012). (bartleby.com)
  • Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
  • In the present study, we reconstructed large phage genomes from freshwater lakes known to contain bacteria that oxidize methane. (nature.com)
  • Bacteriophages (phages), viruses that infect and replicate within bacteria, are important in both natural and human microbiomes because they prey on bacterial hosts, mediate horizontal gene transfer, alter host metabolism and redistribute bacterially derived compounds via host cell lysis 1 . (nature.com)
  • We also widen the research to the genomes of all the bacteria, finding 227 entries in 12 bacterial phyla. (bvsalud.org)
  • The Type VI Secretion System (T6SS) is a multiprotein device that has emerged as an important fitness and virulence factor for many Gram-negative bacteria through the injection of effector proteins into prokaryotic or eukaryotic cells via a contractile mechanism. (frontiersin.org)
  • Bacteria transmit signals through reversible phosphorylation using the classical two component systems, and recent genomic data, in particular from the Ocean Genome Sampling Initiative, have shown that bacteria also rely on Ser/Thr kinases for cell signaling. (seattlechildrens.org)
  • Here we report the 2.1 Å X-ray structure and molecular function of ClbS, a gene product that confers resistance to colibactin toxicity in host bacteria and which has been shown to be important for bacterial viability. (omicsdi.org)
  • Genes coding for glycosaminoglycan-acting lyase, unsaturated glucuronyl hydrolase, and the phosphotransferase system are assembled into a cluster in the genome of pathogenic bacteria, such as streptococci and clostridia. (rcsb.org)
  • However, in the anthrax-causing bacteria B. anthracis, deletion of iron-regulated surface determinant (Isd) genes, which code for surface proteins that bind heme, did not reduce B. anthracis virulence in animal models of infection. (grantome.com)
  • A hallmark of Isd systems is the presence of a conserved protein module termed the near-iron transporter (NEAT) domain, which mediates the transfer of heme into Gram-positive pathogenic bacteria. (grantome.com)
  • In 2014, the MetaHIT consortium by bacteria of the genera Lacto- alytical method used: 66 from 16S published an integrated catalogue bacillus , Prevotella , and Sneathia , rDNA sequencing [11] or 57 from of 10 million bacterial genes de- whereas for infants delivered by whole-metagenome sequencing [5]. (who.int)
  • The involvement of viral DNA-binding proteins in the regulation of virulence genes, transcription, DNA replication, and repair make them significant targets. (mdpi.com)
  • A limited study had indicated that in Sinorhizobium meliloti the YbeY ortholog regulates the accumulation of sRNAs as well as the target mRNAs, raising the possibility that YbeY may play a previously unrecognized role in bacterial sRNA regulation. (biomedcentral.com)
  • Your second year builds on this knowledge and covers areas such as gene regulation, cell biology and metabolism. (kent.ac.uk)
  • Dirk Schübeler has done important work in epigenomics and regulatory genomics, in particular with the dynamics of genome-wide methylation patterns, their role in gene regulation, and the interplay between chromatin state and the actions of transcription factors. (unibas.ch)
  • Degradations of proteins to amino acids and regulation. (unizg.hr)
  • Selenomethionyl SAD and MAD were the mainstays of structural genomics efforts to populate the PDB with novel proteins. (iucr.org)
  • Silver Age of GOLD Introduces New Features The Genomes OnLine Database makes curated microbiome metadata that follows community standards freely available and enables large-scale comparative genomics analysis initiatives. (doe.gov)
  • Loop Genomics has launched its LoopSeq Bacterial Genome Sequencing Kit and Service for de novo bacterial genome assembly using synthetic long reads on Illumina sequencers. (genomeweb.com)
  • The mutational processes that generate this variation are understood, and sequence data have been successfully used in the study of bacterial epidemiologic features, population biology, and evolution ( 16 ). (cdc.gov)
  • However, high-throughput gene sequencing of structural genes ( 19 ) and host immune system protein targets ( 20 ) in M. tuberculosis isolates indicated low levels of sequence diversity. (cdc.gov)
  • The E. histolytica U3 snoRNA (Eh_U3 snoRNA) has showed sequence and structural features similar to Homo sapiens U3 snoRNA. (wikipedia.org)
  • A single transcript chosen for a gene which is the most conserved, most highly expressed, has the longest coding sequence and is represented in other key resources, such as NCBI and UniProt. (ensembl.org)
  • Integrating sequence variation and protein structure to identify sites under selection. (openwetware.org)
  • While the number of sequenced archaeal viruses is increasing, very little sequence similarity has been detected between bacterial and eukaryotic viruses. (edu.au)
  • Discovering the function of unknown proteins is difficult, particularly for species-specific proteins, for which sequence-based methods of annotation fail. (seattlechildrens.org)
  • Combined with structural biology, we discover new protein families and explore even the most divergent sequence space. (seattlechildrens.org)
  • The next thing we're going to learn is to visualise the nucleotides and amino acid sequence of this gene in a separate Window. (futurelearn.com)
  • And here opens a new Window, where we can see the amino acid sequence of the File- of the protein fbaB. (futurelearn.com)
  • In 2008, the first comprehensive sequence of a cancer genome was reported, ushering in a new era of molecular diagnostic, prognostic and therapeutic advances informed by an essential framework to understand cancer's complexities. (nature.com)
  • c) Genome is further categorized into type of nucleic acid, size of the genome, linear or circular, number and size of segments, nucleotide sequence, presence of isomerization, etc. (botnam.com)
  • We have applied a multipronged approach of loss-of-function studies, genome-wide mRNA and sRNA expression profiling, pathway analysis, target prediction, literature mining and network analysis to unravel YbeY-dependent molecular responses of E. coli exposed to hydroxyurea (HU). (biomedcentral.com)
  • Biochemical approaches will define the molecular and structural factors of the NEAT domain of BAS0520. (grantome.com)
  • isolated a material by chemical means from the diseased tobacco leaves, which appeared to be a protein of high molecular weight. (botnam.com)
  • Bawden purified the tobacco mosaic virus and found it to be a crystalline nucleo-protein of very high molecular weight, retaining its infectiousness even when diluted to a concentration of 1/1,000,000. (botnam.com)
  • Since then, protein of high molecular weight possessing all the properties of the respective virus has been isolated and studied. (botnam.com)
  • Professor of molecular and cellular biology Brian Kobilka , PhD, told the audience about his work with a class of cell-surface molecules, a field that has become exceedingly important: "My research on G-protein-coupled receptors began in the late 1980s, at a time when we knew there were maybe seven or eight members of this family," said Kobilka, the Hélène Irwin-Fagan Chair in Cardiology. (stanford.edu)
  • TtSmtB, the only ArsR/SmtB member present in the genome of Thermus thermophilus HB27, was chosen as a model to shed light into such molecular mechanisms at high temperature. (cirn-na.com)
  • Detailed knowledge at molecular level of protein-metal interaction is remarkable to design metal binding domains as scaffolds in metal-based therapies as well as in metal biorecovery or biosensing in the environment. (cirn-na.com)
  • Here, I discuss how we - myself, my laboratory and the diffraction community - have faced the phase problem, considering the evolution of methods for phase evaluation as structural biology developed to the present day. (iucr.org)
  • There, Grundner discovered his interest in Mycobacterium tuberculosis (Mtb) , using structural biology and chemical tools to understand Mtb virulence mechanisms, work for which he received fellowships from the American Lung Association and the New York Community Trust, Heiser Fund. (seattlechildrens.org)
  • The most important contribution that we in the university can make to the success of the biopharmaceutic industry is through work that is done quite independently of them, and without any of their needs in mind," said Roger Kornberg , PhD, Stanford Medicine professor of structural biology, who won the Nobel Prize in chemistry in 2006. (stanford.edu)
  • Targeting of coding messenger RNAs (mRNAs), rather than the proteins in which they encode, has also emerged as a new therapeutic strategy for affecting the biology of as-yet undruggable protein targets. (umich.edu)
  • Projects in the lab range from the development of new high-throughput screening technologies for RNAs and RNA-protein interactions to rational drug design, structural biology and chemical biology. (umich.edu)
  • The complete genome sequences ( 15 - 18 ) provide access to all regions of the chromosome and facilitate such studies. (cdc.gov)
  • The SH1 genome is unique and, except for a few open reading frames, shows no detectable similarity to other published sequences, but the overall structure of the SH1 virion and its linear genome with inverted terminal repeats is reminiscent of lipid-containing dsDNA bacteriophages like PRD1. (edu.au)
  • Genome sequences of several strains from our bacterial collection from the marine, arctic environment have revealed their ability to synthesize different Sias, and represent a novel source for the prospecting of new gene variants. (uit.no)
  • In this paper, we present the draft genome sequences of six Philippine E. mallotivora isolates to provide insights into the genes involved in host-pathogen interactions and compare their genomes to other Erwinia species. (qld.gov.au)
  • All archaeal and many bacterial genomes contain Clustered Regularly Interspaced Short Palindrome Repeats (CRISPR) and variable arrays of the CRISPR-associated ( cas ) genes that have been previously implicated in a novel form of DNA repair on the basis of comparative analysis of their protein product sequences. (biomedcentral.com)
  • An algorithm that can analyse hundreds of millions of genetic sequences has identified DNA-cutting genes and enzymes that are extremely rare in nature. (nature.com)
  • Researchers found that the RNA sequences they analyzed contained a signature gene called RdRp that codes for RNA-dependent RNA polymerase-a complex enzyme designed to catalyze replication. (icr.org)
  • Novel bacterial phyla are often represented by a handful of strains, and the number of species interacting with eukaryotes is likely underestimated. (nih.gov)
  • In this study, 159 bacteriophage strains which had 200 or more orthologous proteins in their genome were examined using the Gene Content Method. (creationresearch.org)
  • A total of 219 French patient isolates and environmental strains were compared using a mixed-genome micro-array. (biomedcentral.com)
  • The Lorraine strain and related Dutch and French Legionella strains contain a marker that encodes a RtxA protein which probably is involved in the increased prevalence in clinical isolates. (biomedcentral.com)
  • Due to the psychrophilic nature of many of the bacterial strains they are likely to express cold active enzymes with high catalytic efficiency that additionally renders them as attractive targets for commercial exploitation. (uit.no)
  • The unique inserts in CRISPR show virtually no similarity even between closely related bacterial strains which suggests their rapid turnover, on evolutionary scale. (biomedcentral.com)
  • However, not all strains of a nephritis-associated M protein serotype are nephritogenic. (medscape.com)
  • In the present study, we searched the unreported phage genomic fragments from this lake for genes involved in methane oxidation. (nature.com)
  • Bioinformatic and comparative genomic analyses allowed us to identify genes encoding three candidate antibacterial effectors located within SPI-6 and two candidate effectors located within SPI-19. (frontiersin.org)
  • Comparative genomic analysis using orthologous gene sets led to the identification of conserved genes within the genus and species-specific gene orthologous groups, which collectively provide a baseline for functional genomic studies to determine genes affecting virulence and host specificity. (qld.gov.au)
  • Comparative-genomic analysis of CRISPR and cas genes leads to the hypothesis that the CRISPR-Cas system (CASS) is a mechanism of defense against invading phages and plasmids that functions analogously to the eukaryotic RNA interference (RNAi) systems. (biomedcentral.com)
  • The apparent differences between Ascoviridae and Iridoviridae in host range, virion morphology, and genome configuration, and the similarities in genes and methylation of genomic DNA were discussed. (psu.edu)
  • After cloning and gene heterologous expression in E. coli, the recombinant protein was obtained as high level in soluble form and then purified to homogeneity. (unina.it)
  • Gene cloning and expression were followed by a production of the recombinant protein in a soluble form. (unina.it)
  • We checked the expression at the transcript and protein level, cloned the gene and expressed the recombinant protein. (bvsalud.org)
  • The recombinant protein was used to affinity-purify specific antibodies from the antiserum. (psu.edu)
  • EhLINE1 encode an endonuclease (EN) protein (in addition to reverse transcriptase and nucleotide-binding ORF1), which have similarity with bacterial restriction endonuclease. (wikipedia.org)
  • Our results suggest that the predicted T3SS genes of V. spinosum could encode a functional T3SS, although further work is needed to determine whether V. spinosum produces a T3SS injectisome that delivers the predicted effectors. (nih.gov)
  • Of manually curated genomes, 22 (18 are complete), ranging from 159 kilobase (kb) to 527 kb in length, were found to encode the pmoC gene, an enzymatically critical subunit of the particulate methane monooxygenase, the predominant methane oxidation catalyst in nature. (nature.com)
  • The recently reported phage genomes range up to 735 kb in length and encode a diversity of genes involved in transcription and translation, as well as genes that may augment host metabolism 2 . (nature.com)
  • In silico analysis of the genome of B. anthracis indicates a non-Isd gene, designated BAS0520, is annotated to encode for a single NEAT-domain protein. (grantome.com)
  • Some phage clusters infected only one family of bacterial hosts, whereas others were more promiscuous. (creationresearch.org)
  • We found that gp21 and the herpesvirus proteases have similarity with proteins forming the tubes of phage tails and bacterial type VI secretion systems, suggesting their common evolutionary origin. (rcsb.org)
  • In addition, pmoC-phage abundance patterns correlate with those of the coexisting bacterial methanotrophs, supporting host-phage relationships. (nature.com)
  • Future work is needed to determine whether phage-associated PmoC has similar functions to additional copies of PmoC encoded in bacterial genomes, thus contributing to growth on methane. (nature.com)
  • Transcriptomics data from Lake Rotsee (Switzerland) showed that some phage-associated pmoC genes were highly expressed in situ and, of interest, that the most rapidly growing methanotroph was infected by three pmoC-phages. (nature.com)
  • The association of the cas genes with CRISPR and, especially, the presence, in CRISPR units, of unique inserts homologous to phage and plasmid genes make us abandon this hypothesis. (biomedcentral.com)
  • Furthermore, the three coliphage genomes showed neither the presence of antibiotic resistance genes nor virulence genes, which makes them desirable candidates for use in phage therapy-based applications. (icar.gov.in)
  • This project aims to increase our fundamental understanding of the roles of bacterial Sias in host-pathogen interactions, and to characterize biosynthetic pathways and enzymes involved in sialic acid synthesis. (uit.no)
  • The structural studies of sialic acid synthesizing enzymes will aid to reveal possible importance of sialic acids for bacterial survival in the host, and in turn enable the development of highly selective antibiotics. (uit.no)
  • We are now extending our chemical proteomic platform to also annotate unknown Pf proteins and to discover enzymes that control life cycle decisions. (seattlechildrens.org)
  • Genes potentially encoding enzymes that participate in carbon, nitrogen and sulfur metabolic pathways were found in greater abundance, than those involved in the oxygen cycle, in the hydrothermal vents. (ucc.ie)
  • Cells depend on their DNA for coding information to make various classes of proteins that include enzymes, certain hormones, transport proteins, and structural proteins that support life. (cdc.gov)
  • This similarity with bacterial protein indicates that transposable elements have been acquired from prokaryotes by horizontal gene transfer in this protozoan parasite. (wikipedia.org)
  • Small regulatory RNAs (sRNAs) play key roles in modulating gene expression in both prokaryotes and eukaryotes. (biomedcentral.com)
  • In other bacterial pathogens, genes important for pathogenicity and host range include secreted protein effectors that suppress host immunity, alter host metabolism, and enable colonization, providing a fitness advantage. (biorxiv.org)
  • This is the first report on the bacterial metabolism of glycosaminoglycan-derived unsaturated uronic acids by isomerase and dehydrogenase. (rcsb.org)
  • The clb gene cluster encodes the biosynthesis of metabolites known as precolibactins and colibactins. (omicsdi.org)
  • Though advances toward elucidating (pre)colibactin biosynthesis have been made, the functions and mechanisms of several clb gene products remain poorly understood. (omicsdi.org)
  • 21.Protein biosynthesis. (unizg.hr)
  • Some microbial pathogens pose even bigger annotation challenges: Less than half of all proteins in Plasmodium falciparum , for example, have known functions. (seattlechildrens.org)
  • Erwinia mallotivora is one of the most important bacterial pathogens of papaya and causes bacterial crown rot disease in the Philippines. (qld.gov.au)
  • Bacterial pathogens must acquire iron to replicate and survive in mammalian hosts. (grantome.com)
  • Bacterial pathogens require iron in order to replicate. (grantome.com)
  • Streptococcal pathogens continue to evade concerted efforts to decipher clear-cut virulence mechanisms, although numerous genes have been implicated in pathogenesis. (cdc.gov)
  • April 28, 2023 - Seattle Children's TB researchers and colleagues uncovered complex microbial protein activity, potentially pointing to new drug targets. (seattlechildrens.org)
  • The most extensively characterized class of bacterial sRNAs are the trans -encoded sRNAs that are encoded distant from the genes for their mRNA targets and that typically have only limited complementarity (10-30 nt) [ 2 ]. (biomedcentral.com)
  • Such interactions generally result in a decrease in protein synthesis, either by stimulating mRNA degradation or by inhibiting translation. (biomedcentral.com)
  • At the ribosome, the processed mRNA is translated to produce proteins from amino acid units. (cdc.gov)
  • Here, we report a 2.0 Å resolution structure of the pentameric procapsid protease of bacteriophage T4, gene product (gp)21. (rcsb.org)
  • It is proposed that unique inserts of CRISPR, some of which are homologous to fragments of bacteriophage and plasmid genes, function as prokaryotic siRNAs (psiRNA), by base-pairing with the target mRNAs and promoting their degradation or translation shutdown. (biomedcentral.com)
  • Functional profiling of the viromes, resulted in the discovery of genes encoding proteins involved in bacteriophage capsids, DNA synthesis, nucleotide synthesis, DNA repair, as well as viral auxiliary metabolic genes such as cytitidyltransferase and ribonucleotide reductase. (ucc.ie)
  • RNA codons and protein synthesis. (crossref.org)
  • The organism's major virulence lies in its ability to produce the potent 62-kd polypeptide exotoxin, which inhibits protein synthesis and causes local tissue necrosis. (medscape.com)
  • These similarites include introns, histones, several types of RNA polymerase, and methionine as the first amino acid in protein synthesis. (bartleby.com)
  • These auxiliary metabolic genes participate in the synthesis of phospholipids and nucleotides respectively and are likely to contribute to enhancing the fitness of their bacterial hosts within the hydrothermal vent communities. (ucc.ie)
  • Using a population-based approach that analyzes multiple loci around the chromosome, we demonstrate that neutral genetic variation in genes associated with antimicrobial drug resistance has sufficient variation to construct a robust phylogenetic tree for M. tuberculosis . (cdc.gov)
  • Some propose that retroviruses and the associated mobile genetic elements found in various animal species originated from within the host genome and fulfilled structural and regulatory functions, and were later exogenized. (creationresearch.org)
  • In two larger clusters, phages infecting different bacterial host genera showed no significant genetic difference. (creationresearch.org)
  • There is growing evidence that phages with unusually large genomes are common across various microbiomes, but little is known about their genetic inventories or potential ecosystem impacts. (nature.com)
  • Systematic humanization of yeast genes reveals conserved functions and genetic modularity. (openwetware.org)
  • The firm believes that applications for Omni-C include structural genetic variant detection and generating genome assemblies. (genomeweb.com)
  • Genes for DhuI and DhuD were found to be included in the streptococcal genetic cluster, whereas KduI and KduD are encoded in clostridia. (rcsb.org)
  • The viral genome (total genetic information) can be RNA or DNA. (icr.org)
  • Duchenne muscular dystrophy, or DMD, is a genetic condition in which sufferers lack the ability to produce dystrophin - an essential structural protein in muscles. (thenakedscientists.com)
  • Direct and indirect ionization of DNA is ultimately responsible for the DNA alterations that adversely affect the structural and genetic integrity of the system. (cdc.gov)
  • Currently, it is not known whether orthologous, inhibitory serpin genes retain the same target proteinase specificity within the Drosophilid lineage, nor whether they give rise to non-inhibitory serpin-fold proteins or other, more diverged, proteins. (cnrs.fr)
  • The highly conserved bacterial YbeY RNase has structural similarities to the MID domain of AGOs. (biomedcentral.com)
  • Finally, evolutionary analysis suggested that these auxiliary metabolic genes are highly conserved and evolve under purifying selection, and are thus maintained in their genome. (ucc.ie)
  • What we see in the bacterial flagellum is an evolutionary history. (evcforum.net)
  • Liu and Ochman (2007) demonstrate that the evolutionary history of the bac flag involves gene duplication and divergence, and common ancestry with secretory systems. (evcforum.net)
  • Predicting evolutionary site variability from structure in viral proteins: buriedness, flexibility, and design. (openwetware.org)
  • She has a strong background in evolutionary bioinformatics and examined problems related to the origin and genome evolution of eukaryotic microbes and archaea. (unibas.ch)
  • This "ancient" gene is also found in some eukaryotes 8 and is supposedly a marker of 'deep evolutionary time,' but this cannot be proven-just speculated. (icr.org)
  • Augmentation of bacterial energy generation by auxiliary metabolic genes has been reported for phages with smaller genomes. (nature.com)
  • The type of natural selection on two auxiliary metabolic genes was also analyzed. (ucc.ie)
  • Each antibacterial effector gene is located upstream of a gene encoding a hypothetic immunity protein, thus conforming an effector/immunity (E/I) module. (frontiersin.org)
  • Of note, the genes encoding these effectors and immunity proteins are widely distributed in Salmonella genomes, suggesting a relevant role in interbacterial competition and virulence. (frontiersin.org)
  • The functioning of this system seems to involve integration of fragments of foreign genes into archaeal and bacterial chromosomes yielding heritable immunity to the respective agents. (biomedcentral.com)
  • [ 7 ] In addition, many M protein serotypes do not confer lifetime immunity. (medscape.com)
  • While some effector proteins specifically target bacterial or eukaryotic cells, others can target both types of cells (trans-kingdom effectors). (frontiersin.org)
  • In addition, there is limited information regarding the repertoire of effector proteins encoded within T6SS SPI-6 and T6SS SPI-19 gene clusters in S . Dublin. (frontiersin.org)
  • Tracing the Evolution of Shiitake Mushrooms Understanding Lentinula genomes and their evolution could provide strategies for converting plant waste into sugars for biofuel production. (doe.gov)
  • Certain commensal Escherichia coli contain the clb biosynthetic gene cluster that codes for small molecule prodrugs known as precolibactins. (omicsdi.org)
  • Two proteins (gbs1892 and gbs1891) of Streptococcus agalactiae strain NEM316 were overexpressed in Escherichia coli, purified, and characterized. (rcsb.org)
  • Genome Insider: Methane Makers in Yosemite's Lakes Meet researchers who sampled the microbial communities living in the mountaintop lakes of the Sierra Nevada mountains to see how climate change affects freshwater ecosystems, and how those ecosystems work. (doe.gov)
  • Identifying structural variation in haploid microbial genomes from short-read re-sequencing data using breseq. (openwetware.org)
  • The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)
  • While the mathematics-based direct methods had proved to be inadequate for typical macromolecules, they returned to crack substantial selenium substructures in SAD analyses of selenomethionyl proteins. (iucr.org)
  • RIViT-seq technology could speed up associating transcription factors with their target genes. (doe.gov)
  • Bacterial transcription, postranscriptional modifications. (unizg.hr)
  • The transcription factors of the ArsR/SmtB family are widespread within the bacterial and archaeal kingdoms. (cirn-na.com)
  • Specialized cell structures called ribosomes are the cellular organelles that actually synthesize the proteins (RNA transcription). (cdc.gov)
  • RNA and ribonucleoprotein immunoprecipitation sequencing revealed that LPS induces a strong coupling between differentially expressed genes and HuR-bound genes, and TMs reduced such interactions. (bvsalud.org)
  • We are using high-throughput experimental tools to identify biochemical functions of proteins. (seattlechildrens.org)
  • Predicting bacterial growth conditions from metabolic output by flux balance analysis. (openwetware.org)
  • In addition to structural genes required for tylosin production, the tyl cluster contains three resistance determinants and several regulatory genes. (nih.gov)
  • Loss of ybeY function, which results in a marked resistance to HU, had global affects on sRNA-mediated gene expression. (biomedcentral.com)
  • In this study, three broad host range highly virulent coliphage genomes were sequenced, characterized for infective and lytic potential, and checked for the presence of virulence and resistance genes. (icar.gov.in)
  • clb+ E. coli induce megalocytosis and DNA double-strand breaks in eukaryotic cells, but paradoxically, this gene cluster is found in the probiotic Nissle 1917. (omicsdi.org)
  • Structural elements of nucleic acids. (unizg.hr)
  • RESULTS: We collated 188 orthologues to the D. melanogaster serpins from the other 11 Drosophilid genomes and used synteny to find further family members, raising the total to 226, or 71% of the number of orthologues expected assuming complete conservation across all 12 Drosophilid species. (cnrs.fr)
  • The microbiome, the "other ge- species have no representative in nome" or "second genome" of the culture collections. (who.int)
  • A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)
  • We are already seeing steps in this direction with important data troves coming from the Affordable Care Act's electronic health record mandates and the 1000 genomes project. (openwetware.org)
  • 15.Protein turnover and amino acid catabolism. (unizg.hr)
  • Specifically, we hypothesize BAS0520 is a surface protein that extracts heme from host hemoglobin, thereby promoting heme transport into the bacterial cell and enhancing iron-dependent replication in mammalian hosts. (grantome.com)
  • Here, we browsed the A. baumannii proteome for homologous proteins to the human HuR(ELAVL1), an RNA binding protein containing three RRMs. (bvsalud.org)
  • Precolibactins and colibactins represent a family of natural products that are encoded by the clb gene cluster and are produced by certain commensal, extraintestinal, and probiotic E. coli. (omicsdi.org)
  • This marker encodes for a structural toxin protein RtxA, described for L . pneumophila as a factor involved in virulence and entry in both human cells and amoebae. (biomedcentral.com)
  • Although previously regarded as a simple intermediate between genes and proteins, the completion of the human genome project surprisingly revealed that the vast majority of our genome, over 98%, encodes for non-coding RNAs such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs). (umich.edu)
  • Structural genome annotation of these assemblies has predicted 4489-4749 protein-coding genes. (qld.gov.au)
  • And that is because the annotation for this particular file is - for this particular genome - is in a different file. (futurelearn.com)
  • The predicted TnAV-CP protein was expressed in bacterial expression system and purified to homogeneity. (psu.edu)
  • Other bacterial sRNAs, referred to as cis -encoded sRNAs, are present in close proximity to their targets, such as upstream, opposite of the 5′ UTR of the target, or between two genes in an operon [ 2 ]. (biomedcentral.com)
  • Blencowe's research team studies the mechanisms by which genes are regulated and coordinated to provide critical functions in mammalian cells. (unibas.ch)
  • There are two major, distinct forms of regulatory small RNAs involved in eukaryotic gene silencing: small interfering (si) RNAs and micro (mi) RNAs. (biomedcentral.com)
  • A full structural and functional protein characterization was carried on and in addition, the enzyme was able to form diamond crystals which diffracted at 1.7 Å. (unina.it)
  • This was the first biochemical characterization and structural analysis of a cold-active esterase isolated from the genus Thalassospira. (unina.it)
  • A biochemical approach highlighted protein features with a functional characterization methodology. (unina.it)
  • The X-ray and NMR structural characterization of the recombinant AB-Elavl revealed that the protein maintained the typical ß1α1ß2ß3α2ß4 and three-dimensional organization of eukaryotic RRMs. (bvsalud.org)
  • In the present study, using a multidisciplinary approach, a structural and functional characterization of the protein was performed focusing on its metal interaction and chemical-physical stability. (cirn-na.com)
  • The papers are split about evenly on the subject, but I personally find the evidence in favor of (b) to be more compelling (particularly since we know of an actual example of a secretion system evolving from bacterial flagella, namely, the Buchnera homolog of the flagellar system). (evcforum.net)
  • TMs decreased the association of HuR with genes involved in chemotaxis and immune response, including Cxcl10, Il1b and Cd40, reducing their expression and protein secretion in primary murine bone marrow-derived macrophages and in an LPS-induced peritonitis model. (bvsalud.org)
  • A gene, TnAV-CP, encoding the major structural protein of the Trichoplusia ni ascovirus 2a (TnAV-2a) was cloned by immunoscreening an expression library with antibodies against total TnAV virion proteins. (psu.edu)
  • The purified antibodies reacted strongly with a single protein of ∼52 kDa from the total TnAV virion proteins in a Western blot. (psu.edu)
  • Phages within some of the smaller clusters had a tight range for genome size, ORF number, GC%, and host morphology. (creationresearch.org)
  • In this investigation we rigorously show that SH1, an icosahedral dsDNA virus infecting Haloarcula hispanica, possesses lipid structural components that are selectively acquired from the host pool. (edu.au)
  • Viruses have a core of DNA and a coat of protein in case the host is animal or bacterial while in the case of plant viruses the coat is made of protein but the core is made of RNA . (botnam.com)
  • In the poster section, Joanna Shisler (University of Illinois at Urbana-Champaign [UIUC], Urbana) reported that the modified virus, Ankara, activates nuclear factor κB through the mitogen-activated protein kinase, extracellular signal–regulated kinase (MEK)/extracellular signal–regulated kinase (ERK) pathway, possibly facilitating the host immune response. (cdc.gov)
  • [2] [3] The cells within the biofilm produce the EPS components, which are typically a polymeric conglomeration of extracellular polysaccharides , proteins , lipids and DNA . (wikipedia.org)
  • 7.Membranes structural components: lipids and proteins. (unizg.hr)
  • Genome condensation and packaging are essential processes in the life cycle of viruses. (mdpi.com)
  • Soil Virus Offers Insight into Maintaining Microorganisms Through a collaborative effort, researchers have identified a protein in soil viruses that may promote soil health. (doe.gov)
  • Recent studies have indicated that a number of bacterial and eukaryotic viruses that share a common architectural principle are related, leading to the proposal of an early common ancestor. (edu.au)
  • In addition to human RNAs, RNAs from other organisms, including bacterial RNAs and RNA viruses, are regarded as leading drug targets for the discovery of new infectious disease agents. (umich.edu)
  • Specific functional analogies are drawn between several components of CASS and proteins involved in eukaryotic RNAi, including the double-stranded RNA-specific helicase-nuclease (dicer), the endonuclease cleaving target mRNAs (slicer), and the RNA-dependent RNA polymerase. (biomedcentral.com)
  • Conceptual parallels exist between bacterial and eukaryotic small-RNA (sRNA) pathways, yet relatively little is known about which protein may recognize and recruit bacterial sRNAs to interact with targets. (biomedcentral.com)