• Lactose fermentation In one study that sought to prove that some fermentation produced by L. lactis can hinder motility in pathogenic bacteria, the motilities of Pseudomonas, Vibrio, and Leptospira strains were severely disrupted by lactose utilization on the part of L. lactis. (wikipedia.org)
  • To this scope, genome sequences of both strains were obtained and subjected to in silico analyses. (frontiersin.org)
  • Most dairy lactococcus strains are unable to use alpha-galactosides as a growth substrate, yet many of these strains are known to have beneficial industrial traits. (ru.nl)
  • Phenotypic characterization of all strains showed that the transconjugant has not only acquired the ability to grow well in soy milk, a substrate rich in alpha-galactosides, but also has retained the flavor-forming capabilities of the recipient strain L. lactis MG1363. (ru.nl)
  • Lactococcus strains with or without the ysbC gene was tested for resistance against fluoro-orotate on minimal plates. (biomedcentral.com)
  • Only Lactococcus strains without the ysbC plasmid was able to grow on minimal plates with fluoro-orotate. (biomedcentral.com)
  • Diversity in robustness of Lactococcus lactis strains during heat stress, oxidative stress, and spray drying stress. (uva.nl)
  • Researchers at the University of Naples Federico II (Italy) in collaboration with the University of Trento (Italy), Teagasc (Ireland) and the APC Microbiome Ireland SFI Research Centre (Ireland) have analyzed the genetic information from LAB strains (genomes) found in fermented foods and in human faeces to answer these questions. (hospitalprofessionalnews.ie)
  • At least 26 strains of S. thermophilus have been identified and had their genomes sequenced. (wikipedia.org)
  • Research teams have sequenced the genome of two strains of S. thermophilus , CNRZ1066 and LMG13811, and stated that the bacteria are not dangerous. (wikipedia.org)
  • In the experiment, two different strains of bacteria are used to make reduced-fat cheddar cheese: a strain of Lactococcus lactis and a strain of S. thermophilus . (wikipedia.org)
  • in the genome of L. lactis MG1363. (wikipedia.org)
  • Conjugal transfer of Tn6098 was demonstrated from the plant-derived donor strain L. lactis KF147 to the recipient L. lactis NZ4501, a derivative of the dairy model strain L. lactis MG1363. (ru.nl)
  • One copy of this sequence is also present in the L. lactis MG1363 chromosome and represents the sole integration site. (ru.nl)
  • Reverse transcription-PCR analysis confirmed that there were higher levels of transcription of a downstream open reading frame (ORF) in the phage-resistant integrants than in the phage-sensitive strain L. lactis MG1363. (omicsdi.org)
  • This gene was also found to confer phage resistance to L. lactis MG1363 when it was cloned into an expression vector. (omicsdi.org)
  • Fermentation-induced variation in heat and oxidative stress phenotypes of Lactococcus lactis MG1363 reveals transcriptome signatures for robustness. (uva.nl)
  • No virulence factors were detected for any strain and only the non-transferable erm(49) gene, which confers resistance to erythromycin and clindamycin, was identified in the genome of B. longum KABP042. (frontiersin.org)
  • The integration of Tn6098 into the genome of the recipient strain was confirmed by Illumina sequencing of the transconjugant L. lactis NIZO3921. (ru.nl)
  • An in silico analysis of the genome of strain D13 confirmed the pln gene cluster. (bvsalud.org)
  • A putative gene, denoted ysbC , was previously identified by genome sequencing of a Lactococcus lactis strain, but the gene was not annotated in the databases, and no function of the predicted encoded polypeptide was identified. (biomedcentral.com)
  • The cryptic plasmids from this strain were coelectroporated, along with the shuttle vector pSA3, into the plasmid-free host L. lactis LM0230. (omicsdi.org)
  • Strain-Dependent Transcriptome Signatures for Robustness in Lactococcus lactis . (uva.nl)
  • Biologically contained strain of Lactococcus lactis, genetically modified to secrete human Trefoil Factor 1 (hTFF1). (biosafety.be)
  • Samples were then analyzed for the presence of two Lactococcus lactis phage groups (936 and c2) and quantification was done by qPCR. (cdc.gov)
  • Both lactococcal phage groups were found on most swabbed surfaces while airborne phages were detected at concentrations of at least 10(3) genomes/m(3) of air. (cdc.gov)
  • Lactococcus lactis W-37 is highly resistant to phage infection. (omicsdi.org)
  • In addition to pSA3, erythromycin- and phage-resistant isolates carried pSRQ900, an 11-kb plasmid from L. lactis W-37. (omicsdi.org)
  • Here, we present the complete genome sequences of two P335-type phages, Q33 and BM13, isolated in North America and representing a novel lineage within this phage group. (omicsdi.org)
  • The Q33 and BM13 genomes exhibit homology, not only to P335-type, but also to elements of the 936-type phage sequences. (omicsdi.org)
  • The two phage genomes also have close relatedness to phages infecting Enterococcus and Clostridium, a heretofore unknown feature among lactococcal P335 phages. (omicsdi.org)
  • Draft genome sequence of coldtolerant Kurthia gibsonii B83, isolated from spinach leaf. (caluniv.ac.in)
  • A 15-bp direct repeat sequence (TTATACCATAATTAC) is present on either side of Tn6098 in the chromosome of L. lactis KF147. (ru.nl)
  • L. lactis is one of the best characterized low GC Gram positive bacteria with detailed knowledge on genetics, metabolism and biodiversity. (wikipedia.org)
  • This process, called genome erosion or reductive evolution, has been described in several other lactic acid bacteria. (wikipedia.org)
  • Bacteriophages specific to L. lactis cause significant economic losses each year by preventing the bacteria from fully metabolizing the milk substrate. (wikipedia.org)
  • 2020. Large-scale genome-wide analysis links lactic acid bacteria from food with the gut microbiome. (hospitalprofessionalnews.ie)
  • My major area of study is RNA dynamics in bacteria to better understand genome-wide post-transcriptional regulations, their role in the regulation of gene expression and their potential for engineering bacteria. (toulouse-biotechnology-institute.fr)
  • My research concerns predominantly two model bacteria, Lactococcus lactis and Escherichia coli , using a systems biology approach to elucidate the different levels of metabolic regulation and notably genome wide omic regulations. (toulouse-biotechnology-institute.fr)
  • Transcriptome Analysis of a Spray Drying-Resistant Subpopulation Reveals a Zinc-Dependent Mechanism for Robustness in L. lactis SK11. (uva.nl)
  • Many different expression systems of L. lactis have been developed and used for heterologous protein expression. (wikipedia.org)
  • Consistent with the results from comparative genomics (see references above), this resulted in L. lactis losing or downregulating genes that are dispensable in milk and the upregulation of peptide transport. (wikipedia.org)
  • The Q33 and BM13 genomes are organized in functionally related clusters with genes encoding functions such as DNA replication and packaging, morphogenesis, and host cell lysis. (omicsdi.org)
  • Four genes for enzymes, involved in starch degradation were detected in B84 genome: amyL, amyY, glgP and apu, coding cytoplasmic and extracellular alpha-amylases, glycogen phosphorylase and amylopullulanase, respectively. (hal.science)
  • DNA-arrays (or DNA-chips or microarrays) are flat slabs of glass, silicon or plastic onto which thousands of multiple short single-stranded (ss) DNA sequences (corresponding to small regions of a genome) have been attached. (collectf.org)
  • Lactococcus lactis is a gram-positive bacterium used extensively in the production of buttermilk and cheese, but has also become famous as the first genetically modified organism to be used alive for the treatment of human disease. (wikipedia.org)
  • lactis is added to milk, the bacterium uses enzymes to produce energy molecules (ATP), from lactose. (wikipedia.org)
  • A group of researchers modified the genome of this bacterium so it could secrete interleukin 10, a molecule that is helpful to treat inflammatory bowel diseases. (rafts4biotech.eu)
  • Lactose fermentation by L. lactis produces acetate that reduces the intracellular pH of Salmonella, which in turn slows the rotation of their flagella. (wikipedia.org)
  • 2016. Seamless and site-directed mutagenesis of the Saccharomycescerevisae genome using CRISPR-Cas9. (concordia.ca)
  • Using a genome-scale metabolic model of Enterococcus faecalis V583 to assess amino acid uptake and its impact on central metabolism. (uva.nl)
  • It is suggested that the L. lactis supernatant mainly affects Salmonella motility through disruption of flagellar rotation rather than through irreversible damage to morphology and physiology. (wikipedia.org)
  • Using state-of-the-art computational analysis tools, LAB genomes were reconstructed from about 300 foods and nearly 10,000 human faecal samples from different continents, looking at the distribution of LAB in humans based on geographical origin, age and lifestyle. (hospitalprofessionalnews.ie)
  • Pathogens' inhibition was mostly mediated by the production of organic acids, but neutralization experiments strongly suggested the presence of additional antimicrobial compounds in probiotic culture supernatants such as the bacteriocin Lantibiotic B, whose gene was detected in the genome of B. longum KABP042. (frontiersin.org)
  • The use of L. lactis in dairy factories is not without issues. (wikipedia.org)
  • L. lactis is mainly isolated from either the dairy environment, or plant material. (wikipedia.org)
  • To honor Michael, we invite submissions for this Special Issue of Viruses focusing on Michael's interests: virus structures and assembly mechanisms, conformational transitions and structure-function relationships, and genome packaging and virus infection mechanisms. (mdpi.com)
  • Use of non-growing Lactococcus lactis cell suspensions for production of volatile metabolites with direct relevance for flavour formation during dairy fermentations. (uva.nl)
  • lactis B84, capable of utilizing starch as a sole carbon source and producing L(+)-lactate, was isolated from spontaneously fermented rye sourdough. (hal.science)
  • Lactococcus lactis has been demonstrated to be a promising candidate for the delivery of functional proteins because of its noninvasive and nonpathogenic characteristics. (wikipedia.org)
  • In addition to all the perks we have said before, both L. lactis and B. subtilis provide a tremendous appeal to biotechs, as they hold the "food- grade" status by the FDA -meaning that they are either safe for human consumption or that it is okay to come into direct contact with food products. (rafts4biotech.eu)
  • lactis W8, a Potential Nisin-Producing Starter Culture for Indian Traditional Fermented Milk (Dahi). (caluniv.ac.in)
  • Based on its history in food fermentation, L. lactis has generally recognized as safe (GRAS) status, with few case reports of it being an opportunistic pathogen. (wikipedia.org)