Genetics lines cell line. Medical search. Frequent questions

The defects suggest faulty specification of different cell types and result in impairment of processes as diverse as cell proliferation and commitment, cell adhesion and cell recognition. (biologists.com)
Stem cell maintenance in the shoot is under the control of the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop, which is tightly integrated with hormone function, in particular auxin and cytokinin that promote cell differentiation and proliferation, respectively 2 . (nature.com)
WUS is a homeodomain transcription factor (TF) produced in the organizing center (OC) domain of apical meristems and is transported via plasmodesmata into the apical domain (called central zone, CZ) to promote proliferation of stem cells 3 . (nature.com)
The transgenic cell line exhibited enhanced cell proliferation, overexpression of eEF1sigma, eIF4E and eEF2 genes compared with the control cell line. (cdc.gov)

However, many of these human induced pluripotent stem cell (hiPSC) lines banked in repositories or developed in labs likely harbor thousands of undetected mutations, casting doubt on how generalizable the findings made with them can be, according to research published yesterday (August 11) in Nature Genetics . (the-scientist.com)

I generally only accept graduate students through the Medical Genetics or GSAT rotation programs. (ubc.ca)

A cell line can harbor thousands of mutations and still be usable for research as long as those mutations are concentrated in irrelevant noncoding areas or don't hit important genes, Nik-Zainal says. (the-scientist.com)
Next, using an exact algorithm, Heinz, we computed the largest connected protein-protein interaction (PPI) network module of significantly deregulated genes in the two CRC cell lines. (vu.nl)
The DEA and the Heinz analyses suggest 20 Wnt and Ras-MAPK signalling genes being deregulated by AURKA, whereof β-catenin and KRAS occurred in both cell lines. (vu.nl)
Finally, shortest path analysis over the PPI network revealed eight 'connecting genes' between AURKA and these Wnt and Ras-MAPK signalling genes, of which UBE2D1, DICER1, CDK6 and RACGAP1 occurred in both cell lines. (vu.nl)
This study, first, confirms that AURKA influences deregulation of Wnt and Ras-MAPK signalling genes, and second, suggests mechanisms in CRC cell lines describing these interactions. (vu.nl)
to find regulatory genes of the achaete-scute complex (AS-C). The AS-C comprises four proneural genes that confer to cells the ability to become neuroblasts or sensory organ mother cells ( Campuzano and Modolell, 1992 , review). (biologists.com)
Subsequent differentiation allows for rear- analysis of a set of mouse B lineage cell lines rep- rangements of the Ig light-chain (IgL) genes that replace the resenting defined stages of B cell development us- surrogate light-chain genes on the surface of the B cell [8]. (lu.se)
To reduce the risk of analyzing cell line- pathway can be divided into distinct stages based on the specific features, we used several representative cell lines for recombination status of the Ig genes and on the expression each of four major stages in B cell development: pro-B, pre-B, pattern of surface markers and the presence of intracellular proteins [1- 6]. (lu.se)
Real-time PCR analysis of eEF1sigma overexpressing cell line exposed to cadmium showed significant induction of anti-apoptotic genes like Bad and Bcl-2, with a concurrent decrease in the expression of the pro-apoptotic gene, p53 compared with the control cell line. (cdc.gov)

Forces that shape cells and tissues can be produced by the contraction of actin filament (F-actin) meshworks by the molecular motor Myosin II (myosin). (nature.com)
She has a Bachelor of Liberal Arts and Science (Honours) in biology and philosophy from the University of Sydney, and a PhD in plant molecular genetics from the University of Adelaide. (cosmosmagazine.com)

Most of the human induced pluripotent stem cells stored at major cell line repositories and used in research harbor thousands of DNA errors, a study finds, highlighting the need for improved quality control measures. (the-scientist.com)
S tem cell research that uses pluripotent stem cells derived from human skin or blood cells has led to numerous discoveries, aided drug development , and proven useful in gene therapies. (the-scientist.com)
Stem cells reside at the growing tip of meristems, where they differentiate to produce new organs throughout the life of plant and maintain a constant reservoir of pluripotent stem cells. (nature.com)

People with HDGC caused by CDH1 gene mutations are born with one mutated copy of the gene in each cell. (medlineplus.gov)
involving the other copy of the CDH1 gene must occur in the cells of the stomach lining during a person's lifetime. (medlineplus.gov)
When both copies of the CDH1 gene are mutated in a particular cell, that cell cannot produce any functional E-cadherin. (medlineplus.gov)
It is unclear why CDH1 gene mutations primarily occur in the stomach lining and these other tissues. (medlineplus.gov)
PCBP1 is both reasonably highly expressed and has a low Gini coefficient in both tissues and cell lines, and is an excellent novel housekeeping gene. (biorxiv.org)
The glucose transporter isoform 1 (GLUT1) gene encodes a key rate-limiting factor in glucose transport into cancer cells. (uni-regensburg.de)
immature cell is then subjected to negative selection to delete grouped based on their previously defined pheno- self-reactive cells before it leaves the BM to enter peripheral typic features, and a gene expression pattern for lymphoid organs, where it becomes a mature B cell [9]. (lu.se)
Transgenic cell line overexpressing the eEF1sigma gene were generated by transfecting HeLa cells with the expression vector pcDNA 3.1 containing human eEF1sigma cDNA. (cdc.gov)
In summary, the eEF1sigma gene overexpressing cell line exhibited significant resistance to cadmium -induced cytotoxicity and apoptosis. (cdc.gov)

E-cadherin helps neighboring cells stick to one another (cell adhesion) to form organized tissues. (medlineplus.gov)
A lack of E-cadherin also impairs cell adhesion, increasing the likelihood that cancer cells will not come together to form a tumor but will invade the stomach wall and metastasize as small clusters of cancer cells into nearby tissues. (medlineplus.gov)
We analyse two comprehensive transcriptome datasets from human tissues and human-derived cell lines in terms of the expression profiles of the SLC and ABC families of membrane transporters. (biorxiv.org)
The Gini index (coefficient) characterises inequalities of distributions, and is used in a novel way to describe the distribution of the expression of each transporter among the different tissues and cell lines. (biorxiv.org)
Similar trends hold true for the expression profiles of transporters in different cell lines, suggesting that cell lines exhibit largely similar transport behaviour to that of tissues. (biorxiv.org)
By contrast, the Gini coefficients for ABC transporters tend to be larger in cell lines than in tissues, implying that some kind of a selection process has taken place. (biorxiv.org)
The lowest four in tissues are FAM32A, ABCB7, MRPL21 and PCBP1, while the lowest three in cell lines are SF3B2, NXF1 and RBM45. (biorxiv.org)
Thus, it is critical to understand how cells orient the cytoskeleton to produce forces that deform tissues. (nature.com)
Together, our in vivo and in silico data provide a framework for understanding how cells orient force generation, establishing a role for geometrical and mechanical patterning of force production in tissues. (nature.com)
If Extramacrochaetae par-ticipates in cell specification by dimerizing with basic-region-helix-loop-helix proteins, the variety of defects and tissues affected by the insufficiency of extramacrochaetae suggests that helix-loop-helix proteins are involved in many embryonic developmental processes. (biologists.com)
We found significantly higher GLUT1 mRNA expression levels in HCC tissues and cell lines compared with primary human hepatocytes and matched nontumor tissue. (uni-regensburg.de)

Using whole-genome sequencing on drug-exposed cell lines, researchers saw high mutation rates in control cell lines intermittently treated with a compound called CX-5461. (genomeweb.com)
Given that the basic genome of a differentiated organism is constant between cells (and we here ignore epigenomics), what mainly discriminates one cell type from another is its expression profile. (biorxiv.org)
When a stem cell is allocated a cell type it will replicate and divide, and the cells born from it will have mostly the same genome and programming. (cosmosmagazine.com)

E-cadherin has many other functions including acting as a tumor suppressor protein, which means it prevents cells from growing and dividing too rapidly or in an uncontrolled way. (medlineplus.gov)
The loss of this protein prevents it from acting as a tumor suppressor, contributing to the uncontrollable growth and division of cells . (medlineplus.gov)
In summary, our findings suggest that increased GLUT1 expression levels in HCC cells functionally affect tumorigenicity, and thus, we propose GLUT1 as an innovative therapeutic target for this highly aggressive tumor. (uni-regensburg.de)

Using a panel of Kras-driven, p53 restorable cell lines derived from genetically engineered mouse models of lung adenocarcinoma and sarcoma (both of which undergo cell cycle arrest upon p53 restoration), as well as lymphoma (which instead undergo apoptosis), we show that the level of mitochondrial apoptotic priming is a critical determinant of p53 reactivation outcome. (nih.gov)

NIEHS research uses state-of-the-art science and technology to investigate the interplay between environmental exposures, human biology, genetics, and common diseases to help prevent disease and improve human health. (nih.gov)

This protein is found within the membrane that surrounds epithelial cells, which are the cells that line the surfaces and cavities of the body. (medlineplus.gov)
How epithelial cells actively respond to mechanical constraints by adjusting how they generate force is important to understand tissue morphogenesis. (nature.com)
In the Drosophila embryo, apical constriction in a strip of epithelial cells along the ventral midline results in the folding of the tissue and the internalization of ventral cells, forming a ventral furrow (VF). (nature.com)

Researchers make hiPSCs by harvesting somatic cells-often from skin-from a person and then reprogramming them to enter an embryonic-like state. (the-scientist.com)
Stem cells derived from adult somatic cells "will carry the mutational history of their past, as well as of any new mutations that occur when you are reprogramming them or growing them in culture," Nik-Zainal says. (the-scientist.com)
Somatic cells make up our organs and body and are constantly replicating and changing. (cosmosmagazine.com)
This mostly happens to somatic cells, but at this stage it can happen to the germline ones too, albeit at a very low frequency. (cosmosmagazine.com)

She did her undergraduate study at Glasgow, starting with physics and chemistry before coming to genetics. (otago.ac.nz)

Among these are traits that depend on functional meristems, populations of stem cells maintained by the CLAVATA-WUSCHEL (CLV-WUS) negative feedback-loop that controls the expression of the WUS homeobox transcription factor. (nature.com)

He also serves on the Science Advisory Board of the Genetics Policy Institute . (allgov.com)

EBV-immortalized B-cell lines are an indefinite source for genomic DNA, mRNA and proteins from a selected individual. (tau.ac.il)
Additionally, ORF6 inhibits cellular mRNA export, resulting in the remodeling of the host cell proteome, and regulates viral protein expression. (bvsalud.org)

In order to deposit a cell line someplace like HipSci, researchers only have to demonstrate that the stem cells don't have any missing or duplicated chromosomes or other largescale genetic errors-analyses that would miss the myriad single-nucleotide mutations identified in the new paper. (the-scientist.com)
The accumulation of mutations is a function of the number of cell divisions, regardless of whether the mutation is induced by cell division or DNA damage before cell division," the researchers write. (cosmosmagazine.com)

Ultimately, they found that ultraviolet (UV) radiation, possibly linked to sunlight exposure prior to harvesting the cells, was causing significant levels of DNA damage to skin-derived hiPSCs-sometimes inducing tens of thousands of mutations. (the-scientist.com)
Those 'normal' cell lines have as many mutations as [some] cancers," Nik-Zainal tells The Scientist . (the-scientist.com)
The sequencing revealed that 72 percent of the skin cell-derived hiPSCs in the HipSci bank showed signs of mutations. (the-scientist.com)
Further analysis revealed that mutations, especially the BCOR mutations in the blood-derived lines, can occur after reprogramming, meaning they didn't originate from the human donors but rather arose as the cells replicated in the lab, likely through selective pressures the cells experience while growing and dividing in a dish. (the-scientist.com)
But "it's not like you're a bad scientist if your cells acquire mutations," says Loring. (the-scientist.com)
Hákon Jónsson of deCODE genetics, Iceland, and colleagues have found that, on average, identical twins differ by about 5.2 developmental mutations. (cosmosmagazine.com)
these cells don't experience the same accumulation of mutations after birth because they don't replicate as much. (cosmosmagazine.com)
This means that the genetic mutations that amass between identical twins is really dependent on how and when those cells are assigned jobs. (cosmosmagazine.com)

As the cells multiply, they can split into two individuals before they become embryos that have exactly the same genetic material (or so we thought). (cosmosmagazine.com)

Malignancies involving mesothelial cells that normally line the body cavities, including the pleura (see the images below), peritoneum, pericardium, and testis, are known as malignant mesothelioma. (medscape.com)

The school appointed him director of the Centre for Early Human Development in 1985, awarded him a personal chair in Obstetrics and Gynaecology/Pediatrics in 1991 and a second personal chair as Professor of Stem Cell Sciences in 2003. (allgov.com)

Here we first performed differential expression analysis (DEA) of AURKA knockdown in two colorectal cancer (CRC) cell lines with 20q gain and AURKA overexpression. (vu.nl)

Functional studies revealed that unlike GL7- TRM subsets that were mostly (RAR-related Orphan Receptor gamma T) RORÎ³T+, GL7+ TRM cells exhibited higher levels of (T-box expressed in T cells) T-bet and Gata-3, corresponding with increased synthesis of interferon-Î³, interleukin-13, and interleukin-5, inherent to both T helper 1 (TH1) and TH2 functions. (bvsalud.org)

genetics of a complex differentiation process. (lu.se)
Cluster of differentiation CD4+ resident memory T (TRM) cells are known to be crucial for this protection, but the diversity of lung CD4+ TRM cells has yet to be fully delineated. (bvsalud.org)

According to study coauthor and Cambridge Biomedical Research Campus medical geneticist Serena Nik-Zainal , the study reveals that the level of quality control involved in such stem cell research may not be up to snuff. (the-scientist.com)
It's a problem, but it's solvable," says Jeanne Loring , a stem cell researcher at the Scripps Research Institute who didn't work on the study. (the-scientist.com)
As it stands now, scientists using these hiPSCs for research or clinical applications only need to screen and characterize their cell lines, whether they're developed in-house or taken from a repository, to whatever extent is required by a journal or the reviewers assessing their work. (the-scientist.com)
Tanis is a Research Fellow based in the Cancer Genetics Lab. (otago.ac.nz)
Genetics research and development must be accompanied by public education and debate involving all relevant sectors of society. (who.int)

In the Drosophila thorax, opposing gradients of Dachsous and Four-jointed expression, which are constituents of the Fat/Dachsous/Four-jointed planar cell polarity pathway, result in the polarized localization of the myosin Dachs 9 . (nature.com)

The aim has been to ensure that these values can be faithfully incorporated in WHO's policies and cooperation work in areas of genetics related to human health. (who.int)
Negative perceptions of science can detract from the beneficial applications of genetics to human health. (who.int)

But every time a cell replicates, there is a chance a mutation will occur. (cosmosmagazine.com)

The control of plant stem cells is essential for sustaining the function of apical meristems, plant growth, and ultimately productivity 1 . (nature.com)

Previous work has shown that gradients or asymmetries in biochemical signals at the cell and tissue level can serve as instructive cues to pattern force generation. (nature.com)
Accelerated glycolysis is one of the biochemical characteristics of cancer cells. (uni-regensburg.de)

We hypothesized that high mitochondrial priming prior to p53 reactivation would lead to apoptosis, while low priming would lead to survival and cell cycle arrest. (nih.gov)
Cells with high initial priming (e.g., lymphomas) lacked sufficient reserve antiapoptotic capacity and underwent apoptosis after p53 restoration. (nih.gov)
Contrary to what we learned in high school genetics, germline DNA can mutate even after a zygote is formed. (cosmosmagazine.com)
cells are activated by interaction with antigens and obtain T indicated that the cell lines representing a defined cell help, they mature into terminally differentiated plasma stage generally presented a high similarity in over- cells secreting large amounts of antibodies [10 -12]. (lu.se)

Why don't these cells keep on dividing, repairing and renewing the organ forever? (creation.com)

During Drosophila gastrulation, actomyosin contraction in ventral cells generates a long, narrow epithelial furrow, termed the ventral furrow, in which actomyosin fibres and tension are directed along the length of the furrow. (nature.com)

7 The differential reductions in the potencies of SLIGRL-NH2, compared with trypsin in the PAR2PRR and PAR2NET cell lines point to differences between the interactions of the trypsin-revealed tethered ligand and the free receptor-activating peptide with PAR2. (nih.gov)

It's a call that's finally, increasingly being communicated: to analyze the cells you're going to use by genomic analysis. (the-scientist.com)

B lymphocyte development is a highly ordered process pro- expression analysis without the use of intermediate amplifica- ceeding from the progenitor cells in the bone marrow (BM) to tion steps. (lu.se)
Phenotypic analyses for markers of lymphocyte memory and residence demonstrated that GL7+ T cells are a subset of CD4+ TRM cells. (bvsalud.org)

Cell lines were evaluated for their sensitivity (calcium signalling) towards trypsin and the receptor-activating peptides, SLIGRL-NH2, SLIGEL-NH2, trans-cinnamoyl(tc)-LIGRLO-NH2, and SFLLR-NH2. (nih.gov)

Trounson's fame spread world wide in 1998 when Trounson's team became one of two racing to be the first to cultivate embryonic stem cell lines (a team from the University of Wisconsin won, narrowly). (allgov.com)

Expression of eEF1sigma in the transgenic cell line was determined by real-time PCR and Western blot analysis. (cdc.gov)

There isn't a way to avoid it," except by stopping cells from dividing altogether, "which is not what you want," she adds. (the-scientist.com)
Altogether there are four main datasets, namely 409 "SLCs" in 59 tissue types and 56 cell lines, and 48 ABCs in the same tissue types and cell lines. (biorxiv.org)

Reactivation of p53 in established tumors typically results in one of two cell fates, cell cycle arrest or apoptosis, but it remains unclear how this cell fate is determined. (nih.gov)
Eukaryotic translation elongation factor 1delta overexpressing cells are resistant to cadmium -induced toxicity and apoptosis. (cdc.gov)
Cadmium induced apoptosis was also studied using the control and eEF1sigma overexpressing cell lines. (cdc.gov)
The eEF1sigma overexpressing cell line exhibited significantly higher resistance to cadmium -induced apoptosis compared with the control cell line. (cdc.gov)

After recovery from pneumococcal infections, we identified a distinct subset of CD4+ T cells defined by the phenotype CD11ahiCD69+GL7+ in mouse lungs. (bvsalud.org)

What they do is affect the pre-cancerous cells without alerting the cancer's stress response mechanisms. (otago.ac.nz)

Flanking the CZ is the peripheral zone (PZ) where cells are recruited for the initiation of new lateral primordia. (nature.com)

These combinations may also be effective much longer because they maintain the normal cells while working 'quietly' in the background. (otago.ac.nz)
Strong background in genetics and stats/bioinformatics preferred. (ubc.ca)

One letter difference in the DNA sequence can draw the line between someone getting ill or not. (otago.ac.nz)

Hypoxic conditions further increased GLUT1 expression levels in HCC cells, and this induction was dependent on the activation of the transcription factor hypoxia-inducible factor-1alpha. (uni-regensburg.de)

Accordingly, suppression of GLUT1 expression by siRNA significantly impaired both the growth and migratory potential of HCC cells. (uni-regensburg.de)
Cadmium -induced cytotoxicity was significantly lower in the eEF1sigma overexpressing cell line compared with the control. (cdc.gov)

The internalized ventral cells give rise to the mesoderm that differentiates into muscle and other internal cell types such as the fat body, macrophages or lymph glands. (nature.com)
We have two different types of cell lines: somatic and germline. (cosmosmagazine.com)

Cells with low initial priming (e.g., lung adenocarcinoma and sarcoma) survived and proceeded to arrest in the cell cycle. (nih.gov)

however, because this type of cancer is hidden underneath the stomach lining, it is usually not diagnosed until the cancer has become widely invasive. (medlineplus.gov)

Tanis' work uses both cell lines and patient tissue samples. (otago.ac.nz)

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