• assay
  • Furthermore, we examined the interaction between MdCRY2 and AtCOP1 using a yeast two-hybrid assay and a bimolecular fluorescence complementation assay. (biomedsearch.com)
  • VvGST4 also gave a similar level of complementation in the maize bombardment assay to these GSTs (Alfenito et al. (nih.gov)
  • Few key regions were identified as being unique to anthocyanin-transporting GSTs, with other single or double amino acid residues differing between GSTs with low and high activity in the bz2 complementation assay. (nih.gov)
  • Molecular
  • GRE Subject Biochemistry, Cell and Molecular Biology was a standardized exam provided by ETS (Educational Testing Service) that was discontinued in December 2016. (wikipedia.org)
  • Molecular phylogenetic analysis revealed P. stipticus to have a close genetic relationship with members of the genus Mycena. (wikipedia.org)
  • Recombination supplies two fault-tolerance mechanisms at the molecular level: recombinational DNA repair (promoted during meiosis because homologous chromosomes pair at that time) and complementation (also known as heterosis, hybrid vigor or masking of mutations). (wikipedia.org)
  • Reflecting this cross-species genetic complementation method, the gene was called "Excision repair cross-complementing 4" The human ERCC4 gene encodes the XPF protein of 916 amino acids with a molecular mass of about 104,000 daltons. (wikipedia.org)
  • proteins
  • An emc allele was identified from a genetic screen designed to identify factors that, when overexpressed, cooperate with mutations in rbf1 , which encodes one of the two Rb proteins found in Drosophila . (biologists.org)
  • Our current results indicate that the genetic determinants such as those involved in the fimbrial biogenesis and regulation, global regulators, transporter proteins, prophage-derived proteins, and enzymes of different functions, to name a few, may play a role in the regulation of type 1 fimbrial expression in response to solid agar and static broth culture conditions. (nih.gov)
  • The ability of VvGST1 and VvGST4 to transport anthocyanins was confirmed in the heterologous maize bronze-2 complementation model, providing further evidence for their function as anthocyanin transport proteins in grape cells. (nih.gov)
  • Mutation
  • Geneticists often refer to wild type and mutant alleles where the mutation is implicitly deleterious and may talk in terms of genetic enhancement, synthetic lethality and genetic suppressors. (wikipedia.org)
  • This approach, the specific locus test (SLT) allowed Russell to study a wide range of specific mutations and to calculate the mutation rates induced by radiations. (wikipedia.org)
  • gene
  • ATM, which has a transcript of 12 kilobases, was found to be mutated in AT patients from all complementation groups, indicating that it is probably the sole gene responsible for this disorder. (nih.gov)
  • Complementation will not occur if the mutations are in the same gene. (wikipedia.org)
  • A genetic map can then be created using linkage and genetic markers, and then the gene of interest can be cloned and sequenced. (wikipedia.org)
  • Discovering disease loci using old forward genetic techniques was a very long and difficult process and much of the work went into mapping and cloning the gene through association studies and chromosome walking. (wikipedia.org)
  • Multiple independent complementation groups of Chinese hamster ovary (CHO) cells have been isolated, and this gene restored UV resistance to cells of complementation group 4. (wikipedia.org)
  • In this sense, epistasis can be contrasted with genetic dominance, which is an interaction between alleles at the same gene locus. (wikipedia.org)
  • mutants
  • We also find fusions of the ventral neurocranium for all midline mutants tested, which may reveal a hitherto unrecognized function of the midline in influencing differentiation of neural crest cells at their destination. (zfin.org)
  • Eight-two mutants were obtained from 7,239 clones screened using the yeast agglutination test. (nih.gov)
  • Tests involving A1 mutants with various deleted amino acid sequences have shown that editing activity is dependent on residues 14 to 35. (wikipedia.org)
  • Plasmids
  • To test whether a methylation-sensitive restriction system contributes to poor B. burgdorferi transformability, shuttle plasmids were treated with the CpG methylase M.SssI prior to the electroporation of a variety of strains harboring different putative R-M systems. (umassmed.edu)
  • strains
  • Low-passage-number infectious strains of B. burgdorferi exhibit extremely low transformation efficiencies-so low, in fact, as to hinder the genetic study of putative virulence factors. (umassmed.edu)
  • As a control, 16S rRNA was constantly expressed in all the strains tested. (nih.gov)
  • It attributes the poor performance of inbred strains to loss of genetic diversity, with the strains becoming purely homozygous at many loci. (wikipedia.org)
  • arises
  • Further, the selective advantage of complementation that arises from outcrossing may largely account for the general avoidance of inbreeding in nature (e.g. see articles Kin recognition, Inbreeding depression and Incest taboo) [citation needed]. (wikipedia.org)
  • yeast
  • His Ph.D. advisor at MIT was David Botstein, and the title of his thesis was "Genetic analysis of the yeast microtubule cytoskeleton. (wikipedia.org)
  • loci
  • Genetic tests demonstrate that Rmr6 encodes a common component required for establishing paramutations at diverse maize loci. (genetics.org)
  • Genetic-linkage studies were able to map the disease loci in cystic fibrosis to chromosome 7 by using protein markers. (wikipedia.org)
  • In general, epistasis is used to denote the departure from 'independence' of the effects of different genetic loci. (wikipedia.org)
  • forward genetic
  • Depending on the region being assessed, forward genetic screens can be classified as illustrated in Figure 2 as: Region Specific screens: Studies are designed specifically to obtain a gradient of phenotypes by generating an allelic series which are helpful in studying the region of interest. (wikipedia.org)
  • variation
  • August Weismann picked up the thread in 1889, arguing that sex served to generate genetic variation, as detailed in the majority of the explanations below. (wikipedia.org)
  • mutant
  • Deletion tests with mutant strands have shown that residues 181 to 210 are integral to mRNA editing, and there is most likely a beta-turn at proline residues 190 and 191. (wikipedia.org)
  • modification
  • Biotechnology - new and sometimes controversial branch of biology that studies the manipulation of living matter, including genetic modification and synthetic biology. (wikipedia.org)
  • Evolutionary
  • Since hypotheses for the origins of sex are difficult to test experimentally (outside of Evolutionary computation), most current work has focused on the maintenance of sexual reproduction. (wikipedia.org)
  • respectively
  • however, in recent versions of the test, the maximum and minimum reported scores have been 760 (corresponding to the 99 percentile) and 320 (1 percentile) respectively. (wikipedia.org)