• Gene transfer systems that have been extensively studied in bacteria include genetic transformation, conjugation and transduction. (wikipedia.org)
  • Bacterial conjugation has been extensively studied in Escherichia coli, but also occurs in other bacteria such as Mycobacterium smegmatis. (wikipedia.org)
  • Bacteria - including the ones carrying antimicrobial resistance genes - also enter the wastewater system. (elifesciences.org)
  • This allowed them to identify both the genomes of species within the community and the mobile genetic elements that can be transferred between bacteria. (elifesciences.org)
  • The relative abundance of these types changed slightly over time, which could be linked to changes in resistant entities within the community, either due to the transfer of such genes, to changes in the composition of bacteria, or both. (elifesciences.org)
  • One of these was a gene called YojI, which encodes resistance to microcin, a common toxin that is widely produced by bacteria and other prokaryotic species. (elifesciences.org)
  • next focused on two relevant types of mobile genetic elements that convey antimicrobial resistance genes: plasmids (small, circular DNA molecules) and bacteriophages (viruses that infect bacteria). (elifesciences.org)
  • But it is our genetic background that influences how bacteria actually function in the human gut. (washington.edu)
  • What's more, bacteria themselves express different genes and make proteins that may predispose certain individuals to gut inflammation or other conditions. (washington.edu)
  • The bacteria were tested for siderophore and indole-3-acetic acid (IAA) production, phosphate solubilization, and presence of the nif H gene. (scielo.br)
  • More than 70% of the evaluated bacteria presented the ability of siderophore production and phosphate solubilization, and possessed the nif H gene. (scielo.br)
  • Since the time of Lane's writing, advances in computers are making it possible to track the transmission of genes between different species of bacteria. (shortform.com)
  • A troubling implication of the study is that if a species of bacteria develops a resistance to antibiotics, it's able to pass that genetic resistance to other bacterial species . (shortform.com)
  • While complex life forms like ourselves can only pass genes to our descendants, harmful bacteria can pass their mutations to neighboring species, accelerating microbial evolution faster than complex life can adapt to protect itself. (shortform.com)
  • Her research is focused on non-coding RNA-dependent molecular mechanisms of gene regulation at the bacteria/host interfaces and research and development of molecular diagnostics for Lyme disease. (lymediseaseassociation.org)
  • Metagenomic DNA from both soil samples was extracted and used to amplify the 16S rRNA gene by using primers deduced from the division bacteria. (conicet.gov.ar)
  • Out of 945 taxa examined, the RAs of 8.8% of taxa had non-zero heritability estimates suggesting that the abundances of those bacteria are influenced by host genetic variation. (biomedcentral.com)
  • In fact, quantitative trait locus (QTL) approaches have successfully identified variation in candidate host genes that influence the RA of specific bacteria not only in Drosophila and mice but also in humans [ 22 ]. (biomedcentral.com)
  • To this end, we propose to construct "robust" genetic sensors and circuits that endow bacteria with the ability to sense environmental signals (e.g., temperature, light, pH, and oxygen level), perform computational operations, and implement programmed actuations (e.g., generation of toxins to kill parasite eggs). (confex.com)
  • The sequence provides for the first time genetic information on an organism from a major group of bacteria not previously genotyped: the bacteroides group of gram-negative anaerobes. (the-scientist.com)
  • Plus, how brain zapping could improve mathematical ability, a trick to block the damage done by a stroke, and the bacterial immune system - how bacteria keep themselves safe from harm! (thenakedscientists.com)
  • A major hurdle to studying many bacteria that can't be cultured has been the difficulty of creating a pure sample of a single bacterial cell's genome that is large enough to sequence. (technologyreview.com)
  • Induction of immunerelated genes resulted higher upon incubation with heatinactivated bacteria rather than with live ones. (unimi.it)
  • For bacteria, the assays target the 16S rRNA gene, and for fungi, they target ribosomal RNA genes. (qiagen.com)
  • SMs are organic compounds that are produced by bacteria and fungi that can mediate microbial competition and interaction and therefore influence the composition of the gut microbiota. (hindawi.com)
  • has been identified as the 2nd most consensus (ERIC) sequences which · 60 environmental samples were tak- frequent organism causing ventilator- are common to Gram-negative enteric en throughout the ICU, including associated pneumonia, the 4th most bacteria [11,12]. (who.int)
  • In 2014, the MetaHIT consortium by bacteria of the genera Lacto- alytical method used: 66 from 16S published an integrated catalogue bacillus , Prevotella , and Sneathia , rDNA sequencing [11] or 57 from of 10 million bacterial genes de- whereas for infants delivered by whole-metagenome sequencing [5]. (who.int)
  • Transduction is a common tool used by molecular biologists to stably introduce a foreign gene into a host cell's genome. (wikipedia.org)
  • We undertook whole genome sequencing of E. faecalis associated with bloodstream infection in the UK and Ireland over more than a decade to determine the population structure and genetic associations with hospital adaptation. (nature.com)
  • Microbial genome sequencing provides the opportunity to gain a detailed understanding of the molecular basis for hospital adaptation. (nature.com)
  • Dennis Mangan and colleagues who completed the work decided to make the genome freely available to researchers worldwide by releasing it on the Comprehensive Microbial Resource website. (the-scientist.com)
  • The site is a tool that allows researchers to access all of the bacterial genome sequences completed to date. (the-scientist.com)
  • The P. gingivalis genome contains 2.3 million DNA base pairs forming 2226 genes, many of which are involved in protein synthesis and energy metabolism. (the-scientist.com)
  • As its name suggests, the goal of this project was to sequence and map the genome (collections of all the genes) of man. (panaderiatroyano.com)
  • This microfluidic chip can amplify the genome of single bacterial cells for sequencing-even those that are impossible to grow in the lab. (technologyreview.com)
  • Sequencing the whole genome of single bacterial cells is "really impressive," says Norm Dovichi , a chemistry professor at the University of Washington, in Seattle. (technologyreview.com)
  • TETyper: a bioinformatic pipeline for classifying variation and genetic contexts of transposable elements from short-read whole-genome sequencing data. (cdc.gov)
  • Whole-genome sequencing allows for finer subtyping of pathogens and reveals evolutionary relationships between bacterial isolates, allowing for more rapid outbreak detection and a better understanding of transmission and links between cases. (cdc.gov)
  • For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
  • By sequencing the entire genome, a comprehensive understanding of the genetic composition is obtained. (folkhalsomyndigheten.se)
  • Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)
  • repetitive sequence elements in the members had been in contact with bacterial genome have shown con- patients for several hours. (who.int)
  • The polymerase chain reaction with mixed oligonucleotide primers targeted for conserved sequences of reported ure genes from other species was used to prepare a DNA probe to detect the V. parahaemolyticus ure gene. (microbiologyresearch.org)
  • To examine how gut microbiomes differ among human populations, here we characterize bacterial species in fecal samples from 531 individuals, plus the gene content of 110 of them. (nih.gov)
  • Comparative host studies further indicated host species determined gut microbial assemblies, even if fed with the same food. (deepdyve.com)
  • Advanced tools of 'multi-omics' technology allow researchers to identify species in the human gut and analyze the bacterial genes and protein products that affect our brain health. (washington.edu)
  • The team, led by Barbara Bendlin, PhD, and Frederico Rey, PhD, collected stool samples from participants and used genetic sequencing technology to identify the bacterial species present, and assess the microbial richness and diversity. (washington.edu)
  • It was not possible to distinguish well-defined groups of isolates based on the bromeliad species and microhabitat they inhabit using genetic characterization by rep-PCR. (scielo.br)
  • Genes reveal the history of evolution via commonalities in species' DNA-for example, it's believed that humans and chimpanzees are closely related because we share 99% of the same genetic code. (shortform.com)
  • This makes it impossible to use shared DNA to trace the history of bacterial species since any DNA within a bacterium could have come from its parent or a completely different neighbor. (shortform.com)
  • While lateral gene transfer hasn't been observed in higher, complex forms of life, something like it may have occurred in a species of fruit flies that diverged from their cousins 13 million years ago. (shortform.com)
  • (2024) . Morphological and genetic evidence suggest gene flow among native and naturalized mint species. (lu.se)
  • This work is an analysis of the distribution of gene families in modern species. (pandasthumb.org)
  • Phylogenetic analysis supported the morphological identification of the rodents and revealed a marked genetic difference between the two heterospecific species. (microbial-ecology.net)
  • Each assay is based on an endpoint PCR amplification of a species-specific genetic region of the relevant microbe or a region of an individual microbial gene. (qiagen.com)
  • However, most microbial species from a given habitat cannot be cultured and thus cannot be experimentally characterized in the laboratory. (ncsu.edu)
  • 16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)
  • A catalogue of 178 reference bacterial birth, and the delivery type is the first core of species has been identified genomes distributed among different factor that has an impact. (who.int)
  • Microbial genetics studies microorganisms for different purposes. (wikipedia.org)
  • Aiming to exploit the genetic potential of soils microorganisms from extreme environments to retrieve salinity tolerance genes, the diversity of saline soil was examined and soil metagenomic DNA was used. (conicet.gov.ar)
  • Taken these results altogether, we conclude that, microbial community in that environment were found to be represented by highly diverse microorganisms, interestingly most of them seems to be uncultured. (conicet.gov.ar)
  • After it was discovered that microorganisms have many different physical and physiological characteristics that are amenable to study, they became objects of great interest to geneticists because of their small size … STRUCTURE AND FUNCTION OF GENETIC MATERIAL DNA & RNA DNA=deoxyribonucleic acid RNA=ribonucleic acid Basic building blocks: Nucleotides Phosphate group Pentose sugar Nitrogenous base 3. (panaderiatroyano.com)
  • Genetics - Genetics - Microbial genetics: Microorganisms were generally ignored by the early geneticists because they are small in size and were thought to lack variable traits and the sexual reproduction necessary for a mixing of genes from different organisms. (panaderiatroyano.com)
  • We study the metabolism, physiology, and evolutionary ecology of microbial symbioses and uncultured microorganisms. (ncsu.edu)
  • We combine the study of uncultured microorganisms with genetic, molecular, and biochemical studies on cultivable microorganisms to gain an in-depth understanding of specific metabolic pathways and physiological strategies. (ncsu.edu)
  • Considering the coincidence between production of urease and possession of trh in V. parahaemolyticus , it was concluded that the presence or absence of the ure gene is completely coincident with that of the trh gene in V. parahaemolyticus strains. (microbiologyresearch.org)
  • The results showed that, in all the strains examined, ure and trh were detected on the same Not I fragment, showing that the two genes localise within a relatively small portion of the chromosome DNA. (microbiologyresearch.org)
  • These results suggest that the ure and trh genes are genetically linked in V. parahaemolyticus strains. (microbiologyresearch.org)
  • Sixty-eight bacterial strains were isolated from 3 different microhabitats of V. gigantea and from 2 microhabitats of T. aeranthos bromeliad plants. (scielo.br)
  • 10'000 strains and cow milk as the food matrix for producing and delivering molecular and bacterial diversity to the organism of mice as animal model. (admin.ch)
  • The metabolic fate of these products will be tested in different stages of the life cycle, including pregnancy, in a nutritional study in germ-free mice as well as in isobiotic mice possessing a stable microbiota composed of 12 defined bacterial strains. (admin.ch)
  • All the bacterial strains significantly adhered to Caco-2 cells, with the ?ctsR mutant strain exhibiting the highest adhesion. (unimi.it)
  • For specific genes, a differential transcriptional pattern was observed upon stimulation with different L. plantarum strains, evidencing a possible role of the knocked out bacterial genes in the modulation of host cell response. (unimi.it)
  • Some of the enzymes which generate aromatic radicals that break down most prominent bacterial strains found were isolated and tax- the complex linkages present in lignin to compounds of lower onomically identified using 16S ribosomal RNA (rRNA) se- molecular weight. (lu.se)
  • Identifying structural variation in haploid microbial genomes from short-read re-sequencing data using breseq. (openwetware.org)
  • A statistical method to identify recombination in bacterial genomes based on SNP incompatibility. (cdc.gov)
  • Microbial genetics is a subject area within microbiology and genetic engineering. (wikipedia.org)
  • Genetic Engineering is a field of work and study within microbial genetics. (wikipedia.org)
  • Microbial genetics also has applications in being able to study processes and pathways that are similar to those found in humans such as drug metabolism. (wikipedia.org)
  • Microbial genetics can focus on Charles Darwin's work and scientists have continued to study his work and theories by the use of microbes. (wikipedia.org)
  • Studying evolution by using microbial genetics involves scientists looking at evolutionary balance. (wikipedia.org)
  • Studying the relationship between microbes and the environment is a key component to microbial genetics evolution. (wikipedia.org)
  • While the influences of environmental exposures on microbiome composition are well known, the degree to which host genetics plays a role in structuring microbial communities is less well understood. (biomedcentral.com)
  • The Ecology and Genetics of Microbial Diversity. (panaderiatroyano.com)
  • Learn microbial genetics with free interactive flashcards. (panaderiatroyano.com)
  • Neidhardt, F. C. Top 25 Most Recent Genetic Discoveries in 2018, 13 Important Genetic Engineering Pros And Cons, 6 Major Disadvantages of Genetically Modified Foods, https://www.bioexplorer.net/history_of_biology/genetics/, Top 10 BEST Colleges For Nutrition and Dietetics, Best Colleges For Environmental Engineering, The 25 Most Notable Biology Discoveries of All Times. (panaderiatroyano.com)
  • Benchmark Papers in Microbiology: Microbial Genetics. (panaderiatroyano.com)
  • The Emergence of Bacterial Genetics. (panaderiatroyano.com)
  • Water tanks presented the most abundant diversity compared with phyllosphere and endophytes, probably due to the high nutrient concentration, which promotes an ideal environment for complex microbial communities. (scielo.br)
  • The intestinal tract of the majority of animals including human beings is colonized by complex microbial communities since birth, called the microbiome. (hindawi.com)
  • Vast and complex microbial communities teem on and inside our bodies. (technologyreview.com)
  • The extent of SPM mineralisation is controlled by the chemical composition of the organic component (related to OC composition), the biological capability and capacity of associated microbial communities (biological component), and the physical mechanisms of protection provided by mineral sediment (mineral component) of SPM 15 . (nature.com)
  • Microbiologists need a strong knowledge base in microbial genomics. (cdc.gov)
  • Gut microbial communities represent one source of human genetic and metabolic diversity. (nih.gov)
  • 2013. Stage 0 spurulation gene A (spo0A) as a molecular marker to study diversity of endospore-forming Firmicutes. (unine.ch)
  • Both fish and mice fed TF had significantly lower gut microbial diversity than those fed NF. (deepdyve.com)
  • Conclusions: Our results reveal that thermal processing of food strongly contributes to the reduction of gut microbial diversity and differentially drives microbial alterations in a host-dependent manner, suggesting specific adaptations of host-gut microbiota in vertebrates responding to thermal processing of food. (deepdyve.com)
  • These findings open a window of opportunity to understand the decline in gut microbial diversity and the community variation in human evolution and provide new insights into the host-specific microbial assemblages associated with the use of processing techniques in food preparation in humans and domesticated animals. (deepdyve.com)
  • Indeed, co-evolution between humans microbial communities are revealing factors that affect and the microbiota in the gastrointestinal tract is a much microbial diversity such as host genotype [6] and a range of environmental factors, particularly diet [7, 8]. (deepdyve.com)
  • The Human Microbiome Project, which was launched by NIH in 2007, provided the first glimpse of the microbial diversity of healthy humans and is exploring the possible relationships between particular human diseases and the microbiome. (nih.gov)
  • Segre and Kong found that the microbial population changes during dermatitis flares, and they are exploring whether the microbial diversity can be used to predict when dermatitis flare-ups will occur. (nih.gov)
  • Because there is no adequate diagnostic to predict which treatment will work best, Segre and Kong are investigating whether the microbial diversity of atopic dermatitis can be used to identify the most effective treatment. (nih.gov)
  • Genetic diversity of the bacterial isolates was evaluated by rep-PCR. (scielo.br)
  • Since the estimated size of the cultivable bacterial population ranges between 0.1 and 1%, limited information about the population diversity is available. (conicet.gov.ar)
  • The last decade has also discovered the association between the gut microbial diversity and health as well as the important contribution of the gastrointestinal microbes to the metabolism of the nutrients ingested by humans. (admin.ch)
  • In that context, Westernized life styles are increasingly being characterized by a lower diversity in dietary patterns and gut microbial composition, both phenomena being associated with detrimental effects on human health. (admin.ch)
  • Fermented foods offer a strategic opportunity to promote health by delivering both nutrient and microbial diversity to the human organism. (admin.ch)
  • 35'000 orthologue groups of genes, will be screened in silico for their genetic diversity and functional properties. (admin.ch)
  • Microbial alpha diversity of heterospecific hosts was highest in the Mediterranean forest bioclimatic zone, followed by the Irano-Turanian shrubland, and was lowest in the Sudanian savanna tropical zone. (microbial-ecology.net)
  • However, whether thermal processing of food triggers gut microbial variation remains unknown. (deepdyve.com)
  • The degree to which host genetic variation can modulate microbial communities in humans remains an open question. (biomedcentral.com)
  • Understanding how host genetic variation shapes the microbiome and how the microbiome ultimately functions to modulate host immunity are fundamental questions that are central to fully characterizing the architecture of many common diseases that occur at mucosal surfaces, including those involving the airway. (biomedcentral.com)
  • Culture has irreplaceable advantages for studying emerging bacterial diseases, as it allows antigenic studies, antibiotic susceptibility testing, experimental models, and genetic studies to be carried out, and remains the ultimate goal of pathogen identification. (cdc.gov)
  • In addition to precise target quantification, a 5-plex dPCR run also shows highly specific detection of different microbial water pathogens (see figure Water microbial pathogen detection in 5-plex on QIAcuity ). (qiagen.com)
  • Understanding the roles of ecological, epidemiological and genetic factors, and specifically the host- pathogen molecular interactions, involved in host-to-host transmission and colonisation is essential for us to expose novel opportunities for the control of the pathogen. (biomedcentral.com)
  • Barrier agains t pathogen s gut bacterial genes in the MetaHIT ation when attempting to extrapolate [10], although Proteobacteria, Ver- catalogue were also well represent- results obtained in mouse models to rucomicrobia, and Fusobacteria are ed in the other metagenomes that the situation in humans. (who.int)
  • The process of studying microbial evolution in this way lacks the ability to give a time scale of when the evolution took place. (wikipedia.org)
  • Dietary shifts in humans over time fluence microbial community structure [6, 14], reflecting have presumably occurred in three stages: from an in- the conserved convergence of gut microbiota within a crease in the sharing of plant roots, bulbs, and tubers in host. (deepdyve.com)
  • Defined synthetic microbial communities (SynCom) have been used to dissect the host-microbiota-associated functions using gnotobiotic animals or in vitro cell models. (hindawi.com)
  • However, we are unable to culture 99% of the microbial majority of the gut microbiota and are thus unable to explore the characteristics of all the individual microbes of the community [ 1 ]. (hindawi.com)
  • The colonic microbiome view is controversial, some people Metagenomics of the Human Intes- is the most diverse and also the best consider the gut microbiota to be tinal Tract (MetaHIT) consortium re- characterized microbial community. (who.int)
  • The recent success gut microbial genes, obtained after has fantastic potential, it has only of faecal microbiota transplantation, sequencing whole faecal microbiota been about 10 years since the sci- especially in the context of Clostridi- metagenomes from 124 European indi- entific community first realized its im- um difficile infection, argues for such viduals [4]. (who.int)
  • Under healthy conditions, the in- nomes obtained from individuals crobiota resembles the mother's skin testinal microbiota is considered to on three continents, including 760 microbial community, composed of be a stable community, influenced samples from Europe. (who.int)
  • Our findings provide evidence of host genetic influences on upper airway microbial composition in humans and implicate mucosal immunity genes in this relationship. (biomedcentral.com)
  • The isolates were affiliated to 23 genera representing 8 different suborders based on nearly full length 16S rRNA gene sequencing. (uni-wuerzburg.de)
  • Putative virulence and antibiotic resistance genes were over-represented in L1, L2 and L3 isolates combined, versus the remainder. (nature.com)
  • Gram-positive, spore-forming bacilli comprised most bacterial isolates. (scielo.br)
  • Bacterial metabolism selected lignin model compounds (vanillin, vanillate, 4- hydroxybenzoate, p-coumarate, benzoate, and ferulate) was carried out with three of the Pseudomonas isolates and in addition with the strain Pseudomonas putida KT2440 from a Introduction culture collection. (lu.se)
  • Mutations can occur in any area of the gene. (medscape.com)
  • Murine gene mutations in human counterparts may be associated with a clinical illness different from the illness seen in mice. (medscape.com)
  • Antimicrobial resistance occurs through different mechanisms, which include spontaneous (natural) genetic mutations and horizontal transfer of resistant genes through deoxyribonucleic acid (DNA). (who.int)
  • Diseases and disorders can be associated with misspellings or genetic mutations. (bvsalud.org)
  • Shared features of the functional maturation of the gut microbiome were identified during the first three years of life in all three populations, including age-associated changes in the genes involved in vitamin biosynthesis and metabolism. (nih.gov)
  • The second phase of HMP which started on September 8, 2013, focuses on a survey of the biological properties of the microbiome, such as gene expression profiles, proteins, and metabolites, produced by the microbiome under specific conditions such as in preterm birth or in diabetic patients. (nih.gov)
  • The recent technological advances and utilization of a variety of accurate approaches have further made it possible to monitor and accurately analyze the intestinal microbial community composition and how the functionality and structure of the microbiome vary in healthy and diseased individuals. (hindawi.com)
  • Here, we performed a genetic mapping study of the microbiome in two accessible upper airway sites, the nasopharynx and the nasal vestibule, during two seasons in 144 adult members of a founder population of European decent. (biomedcentral.com)
  • Similar to the traits it influences, the microbiome itself can be considered a complex phenotype with environmental and genetic factors contributing to its composition [ 9 ]. (biomedcentral.com)
  • Although knowledge of the airway microbiome lags behind that of the gut, important characteristics of the microbial communities in the airway are beginning to emerge. (biomedcentral.com)
  • and (2) that the development of host-free microbiome model systems could be useful for rapidly screening pesticides against functionally distinct microbial catalogues of interest. (researchgate.net)
  • We confirmed host phylogeny using the sequencing method and analyzed the influence of host phylogeny and bioclimatic zone parameters on the rodent gut microbiome using high-throughput amplicon sequencing of 16S rRNA gene fragments. (microbial-ecology.net)
  • The Microbiome Modeling Toolbox: from microbial interactions to personalized microbial communities. (cdc.gov)
  • These tools are not only antigenic and serologic assays but also in vitro and in vivo disease models for pathophysiologic studies and antimicrobial susceptibility testing, plus extensive genetic sequencing. (cdc.gov)
  • Microbial communities in wastewater treatment plants provide insights into the development and mechanisms of antimicrobial resistance. (elifesciences.org)
  • identified 29 different major types of antimicrobial resistance genes. (elifesciences.org)
  • Overall, antimicrobial genes that provide protection against multiple drugs, and those that provide resistance against two common types of antibiotics (aminoglycosides and beta lactams) were both abundant and highly expressed. (elifesciences.org)
  • Interestingly, the most highly expressed genes were related to resistance against antimicrobial peptides, which are part of the innate immune system in multicellular organisms. (elifesciences.org)
  • To better understand the mechanisms underlying antimicrobial resistance within microbial communities in wastewater, de Nies et al. (elifesciences.org)
  • q = 0.002), a long non-coding RNA that binds to peptidoglycan recognition protein 3 ( PGLYRP3 ) mRNA , a gene encoding a known antimicrobial protein. (biomedcentral.com)
  • Identification of emerging bacterial pathogens generally results from a chain of events involving microscopy, serology, molecular tools, and culture. (cdc.gov)
  • In the United States, local, state, and federal agencies are uploading data on bacterial foodborne pathogens, influenza, and other pathogens to public databases. (cdc.gov)
  • In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)
  • First, we will test a wide range of individual microbial strainsas well as naturally-occurring soil biota for the ability to induce MDH. (ncsu.edu)
  • Diagram describing the respective places of culture-, polymerase chain reaction-, serology- and histology-based approaches for the diagnosis of acute bacterial infections, according to the natural course of the disease. (cdc.gov)
  • As a consequence, treatment of many common bacterial infections, such as sepsis, or urinary and sexually transmitted infections, is now compromised. (elifesciences.org)
  • In 1952, Colonel Ogden Bruton noted the absence of immunoglobulins (Ig) in a boy with a history of pneumonia and other bacterial sinopulmonary infections. (medscape.com)
  • As Bruton originally described, XLA manifests as pneumonia and other bacterial sinopulmonary infections in 80% of cases. (medscape.com)
  • This undermines the effectiveness of the available treatment options and thus contributes to the persistence of microbial infections. (who.int)
  • Imbalances or changes in the composition of bacterial communities can shift this relationship from symbiotic to pathogenic, a condition known as dysbiosis, which has been implicated in a variety of diseases [ 3 ]. (biomedcentral.com)
  • In mice, the composition of bacterial communities has been linked to body weight and health. (technologyreview.com)
  • Sediment microbes are known for pollution reduction in the face of contamination, making bacterial communities an important area for bioindicator research. (mdpi.com)
  • Results: We found no differences in overall microbial composition and structure in the pairwise NF and TF, but identified differential microbial communities between food and gut. (deepdyve.com)
  • Moreover, thermal processing of food triggered the changes in their microbial communities. (deepdyve.com)
  • While microbial communities in wastewater thrive on the nutrient-rich streams from sewage systems, they also encounter a range of micropollutants arising from human domestic and industrial activity, including antibiotics. (elifesciences.org)
  • On the other hand, a metagenomic approach allows to access to knowledge of a broader range, and to be more representative of the microbial communities. (conicet.gov.ar)
  • Another approach to studying unculturable microbial communities, called metagenomics, is to create an inventory of a community's genes. (technologyreview.com)
  • These inventories are providing insight into complex bacterial communities, but they "lose the basic unit: the cell," says Quake. (technologyreview.com)
  • Single-cell sequencing efforts will greatly expand microbiologists' understanding of the complex bacterial communities living in our bodies, says Anthony Chow , emeritus professor of medicine at the University of British Columbia. (technologyreview.com)
  • Lately, the idea was challenged that most animals harbor beneficial microbial communities. (uni-giessen.de)
  • Our data indicate that normal and inflamed skin harbor distinct phageomes and suggest a causative relationship between changing viral and bacterial communities as a driver of skin pathology. (microbial-ecology.net)
  • Microbial communities are ubiquitous in all environments on Earth that support life, and they play crucial roles in global biogeochemical cycles, plant and animal health, and biotechnological processes. (ncsu.edu)
  • Figure 3: Prevalence of virulence and antibiotic resistance genes in the dominant lineages (L1-L3, n = 89) and remainder ( n = 79). (nature.com)
  • The Parkinson's disease genes pink1 and parkin , which encode a mitochondrially targeted protein kinase, and an E3 ubiquitin ligase, respectively, participate in a key mitochondrial quality-control pathway that eliminates damaged mitochondria. (sdbonline.org)
  • High temperature protein G (HtpG), a gene encoding a bacterial HSP, is less abundant in pediatric CD patients than in healthy individuals. (dal.ca)
  • Third, we will investigate the molecular mechanisms of MDH by measuring gene and protein expression of both hybrid and inbred plants as well as the microbes inside their roots. (ncsu.edu)
  • Many surface protein genes were missing or truncated, unlike immune evasion genes, and several distinct variants were identified. (biomedcentral.com)
  • Morphological, host range, and genetic characterization of two coliphages. (cdc.gov)
  • Genetic characterization of norovirus GII.4 variants circulating in Canada using a metagenomic technique. (cdc.gov)
  • The ways include identifying certain pathways, genes, and functions. (wikipedia.org)
  • Endotoxin triggers humoral enzymatic mechanisms involving the complement, clotting, fibrinolytic, and kinin pathways and causes much of the morbidity in gram-negative bacterial sepsis. (msdmanuals.com)
  • 0.05) and were enriched for genes in mucosal immunity pathways. (biomedcentral.com)
  • A functional analysis of genes born during this Archaean expan- sion reveals that they are likely to be involved in electron-transport and respiratory pathways. (pandasthumb.org)
  • A comprehensive fungi-specific 18S rRNA gene sequence primer toolkit suited for diverse research issues and sequencing platforms. (cdc.gov)
  • Dr. Lybecker's research utilizes interdisciplinary approaches including biochemical, molecular genetic, bioinformatics and immunological to examine microbial disease pathogenesis. (lymediseaseassociation.org)
  • Pronounced differences in bacterial assemblages and functional gene repertoires were noted between US residents and those in the other two countries. (nih.gov)
  • Once the subject is observed, scientist may compare it to a sequence of a conserved gene. (wikipedia.org)
  • The ability to sequence large bacterial collections means that the molecular epidemiology and gene content of epidemic and sporadic lineages can now be defined systematically. (nature.com)
  • An Excel Macro for Determining Allelic and Sequence Types of Bacterial Clones in Multilocus Sequence Typing. (cdc.gov)
  • For human immunodeficiency virus, genetic-sequence data generated for clinical purposes can be analyzed to identify potential clusters for early intervention. (cdc.gov)
  • The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)
  • Most microbial BGCs that have been identified so far contain genes encoding core biosynthetic enzymes such as polyketide synthase (PKS) and nonribosomal peptide synthetase (NRPS). (hindawi.com)
  • Such findings in human and mouse models point to the tantalizing prospect that restoring healthy gut bacterial composition could prevent or slow the development of Alzheimer's in at-risk populations. (washington.edu)
  • PANINI: Pangenome Neighbour Identification for Bacterial Populations. (cdc.gov)
  • 2011. Use of isothermal microcalorimetry assay to characterize microbial oxalotrophic activity. (unine.ch)
  • He looks at the paper and claims it supports this silly idea called front-loading: the Designer seeded the Earth with creatures that carried a teleological evolutionary program, loading them up with genes at the beginning that would only find utility later. (pandasthumb.org)
  • The unsurprising fact that many gene families are of ancient origin seems to him to confirm his weird idea of a designed source, when of course it does nothing of the kind, and fits quite well in an evolutionary history with no supernatural interventions at all. (pandasthumb.org)
  • Here we use an explicit model of macro- evolution including gene birth, transfer, duplication and loss events to map the evolutionary history of 3,983 gene families across the three domains of life onto a geological timeline. (pandasthumb.org)
  • Predicting bacterial growth conditions from metabolic output by flux balance analysis. (openwetware.org)
  • This study explores the pattern of bacterial responses to metal and nutrient pollution loading and seeks to evaluate whether bacterial indicators can be effective as a biomonitoring risk assessment tool for wetland ecosystems. (mdpi.com)
  • More than half a century ago, Heukelekian and Heller [6] showed that at low nutrient concentrations substrate plays an important role in bacterial multiplication. (who.int)
  • The uptake of donor DNA and its recombinational incorporation into the recipient chromosome depends on the expression of numerous bacterial genes whose products direct this process. (wikipedia.org)
  • E. coli conjugation is mediated by expression of plasmid genes, whereas mycobacterial conjugation is mediated by genes on the bacterial chromosome. (wikipedia.org)
  • Attributed to the failure of neurons to clear dysfunctional mitochondria , loss of gene expression leads to loss of nigrostriatal neurons. (sdbonline.org)
  • were able to further analyse gene expression at the level of the entire microbial community. (elifesciences.org)
  • This multi-stability can be explained by stochastic transitioning among multiple stable states of gene expression which are often the results of positive feedback loops in gene regulatory networks. (confex.com)
  • Gene Expression 2. (panaderiatroyano.com)
  • Biochemical regulation of gene expression. (entrance-exam.net)
  • Microbial identification determines the presence or absence of a microbe in a sample, while microbial profiling determines its relative expression under two or more experimental conditions, thus requiring a reference sample and a normalizer. (qiagen.com)
  • Samples were collected over one and a half years, which allowed the researchers to assess the dynamic changes in the inter-relationship between microbes and mobile genetic elements. (elifesciences.org)
  • Thus, to fully exploit microbes in synthetic biology applications, complex gene regulatory systems and their behaviors in diverse conditions need to be better understood. (confex.com)
  • Second, we will map the genetic architecture of MDH to identify genomic loci whose effect on heterosis is dependent on the microbial environment, and test for a genetic correlation with loci underlying resistance to a variety of pathogenic microbes in the field. (ncsu.edu)
  • Many factors have contributed to the persistence and increase in the occurrence of infectious diseases, such as societal changes, health care, food production, human behaviour, environmental change, public health infrastructure and microbial adaptation [2]. (who.int)
  • Natural transformation is a bacterial adaptation for DNA transfer between two cells through the intervening medium. (wikipedia.org)
  • Temperature Adaptation of Aquatic Bacterial Community Growth Is Faster in Response to Rising than to Falling Temperature. (lu.se)
  • Bacterial survival was evaluated in an in vitro system, simulating the transit along the human oro-gastro-intestinal tract. (unimi.it)
  • The microbial degradation of native lignin by basidiomyce- citrant and stable in the environment. (lu.se)
  • Our study highlights the relevance of microbial genetic background in host-probiotic interaction and might contribute to identify candidate bacterial genes and molecules involved in probiosis. (unimi.it)
  • 2013. Gains of bacterial flagellar motility in a fungal world. (unine.ch)
  • ABSTRACT Optical density is a proxy of total biomass concentration and is commonly used for measuring the growth of bacterial cultures. (microbial-ecology.net)
  • Surprisingly, we find that a brief period of genetic innovation during the Archaean eon, which coincides with a rapid diversification of bacterial lineages, gave rise to 27% of major modern gene families. (pandasthumb.org)
  • Moreover, culture provided the basis of other supplemental tools to elucidate the causes of microbial disease and to study the clinical and biological features of emerging bacterial diseases. (cdc.gov)
  • To answer these questions, we examined the key steps that led to identification of most bacterial diseases that have been discovered during the last 20 years. (cdc.gov)
  • Additionally, this review reinforces how various technological advances could be combined with SynCom to have a better understanding of the complex microbial interactions in various gut inflammatory diseases including IBD. (hindawi.com)
  • The parasitic diseases laboratories at CDC are developing a new type of diagnostic test based on the targeted amplification and next-generation sequencing of eukaryotic housekeeping genes. (cdc.gov)
  • We identified several SM-encoding biosynthetic gene clusters (BGCs) from the metagenomic data of lean and obese individuals and found significant association between some BGCs, including those that produce hitherto unknown SM, and obesity. (hindawi.com)
  • Metagenomic sequencing engables a thorough mapping of the genetic material in the clinical sample, providing a broader understanding of the microbiological profile. (folkhalsomyndigheten.se)
  • Interaction with human gut epithelial cells was studied by assessing bacterial adhesive ability to Caco-2 cells and induction of genes involved in innate immunity. (unimi.it)
  • The microbe-microbe interactions can alter the composition of the microbial community and influence host health by producing secondary metabolites (SMs). (hindawi.com)
  • In particular, cells from Δhsp18.55 and ΔftsH mutants strongly triggered immune defence genes. (unimi.it)
  • The results of these experiments will clarify the microbial features and patterns of plant immune activity that result in MDH. (ncsu.edu)
  • Surface and immune evasion genes are candidates for S. aureus vaccines, and their distribution and functionality is key. (biomedcentral.com)
  • 2013. Quantification of endospore-forming Firmicutes by qPCR with the functional gene spo0A. (unine.ch)
  • Meghan Lybecker obtained her Ph.D in the Division of Biological Sciences at the University of Montana in Missoula, MT, where she studied gene regulation and pathogenesis of Borrelia burgdorferi. (lymediseaseassociation.org)
  • Dr. Lybecker merged her research in bacterial pathogenesis and RNA biology as an Associate Professor at the University of Colorado Colorado Springs for seven years. (lymediseaseassociation.org)
  • Bacterial conjugation is the transfer of genetic material between bacterial cells by direct cell-to-cell contact or by a bridge-like connection between two cells. (wikipedia.org)
  • This report presents the status of AMR in Africa by analysing the main types of resistance and the underlying genes where possible. (who.int)
  • We know that bacterial cells are more sensitive to drugs than human cells, so we can target them without hitting human cells. (washington.edu)
  • Microbial identification and profiling are of interest to many fields including human health, food and feed testing and environmental testing. (qiagen.com)
  • In addition, microbial SMs have also been implicated in human physiology, although their precise role in obesity is unclear. (hindawi.com)