Genetic homologous mice knockout. Medical search. Frequent questions

Homologous recombination is a type of genetic recombination in which genetic information is exchanged between two similar or identical molecules of double-stranded or single-stranded nucleic acids (usually DNA as in cellular organisms but may be also RNA in viruses). (wikipedia.org)
Homologous recombination is widely used by cells to accurately repair harmful DNA breaks that occur on both strands of DNA, known as double-strand breaks (DSB), in a process called homologous recombinational repair (HRR). (wikipedia.org)
Homologous recombination also produces new combinations of DNA sequences during meiosis, the process by which eukaryotes make gamete cells, like sperm and egg cells in animals. (wikipedia.org)
Homologous recombination is also used in horizontal gene transfer to exchange genetic material between different strains and species of bacteria and viruses. (wikipedia.org)
Although homologous recombination varies widely among different organisms and cell types, for double-stranded DNA (dsDNA) most forms involve the same basic steps. (wikipedia.org)
Homologous recombination that occurs during DNA repair tends to result in non-crossover products, in effect restoring the damaged DNA molecule as it existed before the double-strand break. (wikipedia.org)
Homologous recombination is conserved across all three domains of life as well as DNA and RNA viruses, suggesting that it is a nearly universal biological mechanism. (wikipedia.org)
The discovery of genes for homologous recombination in protists-a diverse group of eukaryotic microorganisms-has been interpreted as evidence that homologous recombination emerged early in the evolution of eukaryotes. (wikipedia.org)
Since their dysfunction has been strongly associated with increased susceptibility to several types of cancer, the proteins that facilitate homologous recombination are topics of active research. (wikipedia.org)
Homologous recombination is also used in gene targeting, a technique for introducing genetic changes into target organisms. (wikipedia.org)
I-SceI) to cut chromosomes for genetic engineering of mammalian cells, where nonhomologous recombination is more frequent than in yeast. (wikipedia.org)
In 1947, the microbiologist Joshua Lederberg showed that bacteria-which had been assumed to reproduce only asexually through binary fission-are capable of genetic recombination, which is more similar to sexual reproduction. (wikipedia.org)
He developed a technique called homologous recombination that introduced targeted genetic modifications to cells and allowed the creation of so-called 'knockout mice' to help scientists understand how individual genes work. (wral.com)
By the natural process of homologous recombination some of the electroporated stem cells will incorporate the new sequence with the knocked-out gene into their chromosomes in place of the original gene. (wikipedia.org)
This method uses homologous recombination to disable a gene of interest to produce a genetic knockout. (cshl.edu)
Two of these-homologous recombination (HR) and non-homologous end-joining (NHEJ)-are different routes for fixing the supposed damage. (the-scientist.com)
LoxP sites flanking exon 27 and a PGK-neo cassette were introduced via homologous recombination. (jax.org)
AND-34 −/− mice were generated by homologous recombination. (molvis.org)
Inclusion of desired sequences between recombination "arms" that are homologous to the region flanking the DSB allows the "knock in" of the transgene. (genecopoeia.com)
Properly targeted ES cells containing a homologous recombination event were selected, cloned, and injected into blastocysts. (ximbio.com)
Homologous recombination introduced a floxed neomycin resistance cassette into intron 2, and a single loxP site into intron 1. (jax.org)
The CRISPR/Cas9-mediated homologous recombination system with donor DNA oligonucleotides of the human sites of the corresponding gene sites was used to humanize the fourth and fifth exons. (eco-vector.com)
As a result of the study, a strategy for CRISPR/Cas9-assisted homologous recombination in the genome of mouse embryonic stem cells has been developed to create a fully humanized Snca gene encoding α-synuclein, and the clone genome of mouse embryonic stem cells has been edited using a CRISPR technology. (eco-vector.com)
The RNA and DNA oligonucleotides necessary for the creation of RNP complexes that carry out a directed homologous recombination in the Snca locus of the mouse genome have been synthesized. (eco-vector.com)
Furthermore, PARP-1 abrogation leads to increased DNA resection tracks and an increase of homologous recombination in cellulo. (nature.com)
A local high frequency of microhomologies, macrohomologies, tandem repeats, and repeat/repetitive sequences contribute to a high rate of nonallelic homologous recombination involving NEMO , resulting in the development of de novo deletion mutations. (medscape.com)
U.S. Patent 6,410,246 has claims relating to a method for generating a library of yeast expression vectors by exploiting the genetic mechanism of homologous recombination in yeast. (indexarticles.com)
Biocytogen uses its proprietary CRISPR/Cas9-based Extreme Genome Editing (EGE™) system to obtain faster results by increasing the homologous recombination 10 to 20 fold. (renmab.com)
Full-length Rac prophage protein RecE and its partner RecT mediate highly efficient linear-to-linear homologous recombination mechanistically distinct from. (biosave.com)
Red/ET recombination allows the exchange of genetic information in a base pair. (biosave.com)
It is noteworthy that several essential NER factors (RPA1C3, proliferating cell nuclear antigen, and DNA ligase) also play independent functions in other crucial cellular processes, such as DNA replication and homologous recombination. (colinsbraincancer.com)
To track differentiating cell populations, reporter cell lines generated by homologous recombination (knock-in) or via BAC transgenes have been widely used in mouse cells (5-8 ). (lu.se)

In this work, we analyze expression patterns of DNMT3A in patients with IBD, perform loss of function experiments using Caco-2 cell line and generate a conditional Dnmt3a ΔIEC mouse line. (nature.com)
In this study, mouse conditional knockout of VSMC GGTase-I (Pggt1b Δ / Δ mice) was generated using the CRISPR/Cas9 system. (hindawi.com)
Moreover, since more than half of Plasmodium genes are required for normal asexual blood-stage reproduction, and cannot be targeted using knockout methods, we discuss how CRISPR/Cas9 could be used to scale up conditional gene knockdown approaches to systematically assign function to essential genes. (portlandpress.com)
Generation of Bmpr/Alk3 conditional knockout mice. (jax.org)
The aim of the study was to create a genetically modified clone of mouse stem cells with a conditional knockout of humanized α-synuclein, which can be used for the reinjection into mouse blastocysts, as well as for basic and applied in vitro research in the field of pathophysiology and neuropharmacology. (eco-vector.com)
To create mouse stem cells with a conditional knockout of the humanized Snca gene, a previously obtained clone with the first Snca exon flanked by LoxP sites, was used. (eco-vector.com)
The stable nature of the site and the ability for scientists to control global or conditional gene expression make the Rosa26 mouse model a versatile genetic tool. (renmab.com)

We therefore introduce the reader to CRISPR-based screens in the related apicomplexan Toxoplasma gondii and discuss how these approaches could be adapted to develop CRISPR/Cas9 based genome-scale genetic screens in malaria parasites. (portlandpress.com)
Non-uniform dystrophin re-expression after CRISPR-mediated exon excision in the dystrophin/utrophin double-knockout mouse model of DMD. (ox.ac.uk)
Here we have investigated the use of the Staphylococcus aureus CRISPR-Cas9 system and a double-cut strategy, delivered using a pair of adeno-associated virus serotype 9 (AAV9) vectors, for dystrophin restoration in the severely affected dystrophin/utrophin double-knockout (dKO) mouse. (ox.ac.uk)
CRISPR treatment was insufficient to extend lifespan in the dKO mouse, and dystrophin was expressed in a within-fiber patchy manner in skeletal muscle tissues. (ox.ac.uk)
The prokaryote-derived CRISPR (clustered regularly interspaced short palindromic repeats) is a genetic engineering technique in molecular biology by which the genomes of living organisms, including humans, may be modified ( Ledford, 2015 ). (sciendo.com)
Especially, the CRISPR/Cas9 genetic scissors discovered in 2012 by Emmanuelle Charpentier and Jennifer A. Doudna who won the 2020 Nobel Prize in Chemistry have not only revolutionized basic science but also resulted in innovative crops and will lead to ground-breaking new medical treatments ( http://www.nobelprize.org/ ). (sciendo.com)
Traditionally, these knock-in mice were generated using mouse embryonic stem cells, and this process has become more efficient with the advent of CRISPR technology. (renmab.com)
In chapter 2, by using CRISPR/Cas9 gene editing, genetic analysis and biochemical assays, I identified the differential pairings of typical NLR receptor SOC3 with atypical NLR proteins CHS1 or TN2 to guard the homeostasis of the E3 ligase SAUL1. (ubc.ca)

White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)
Chimeric mice were mated with MF1 mice to generate mice heterozygous for GSTP1/2 knockout. (ximbio.com)
Heterozygous mice were interbred to generate mice homozygous for GSTP1/2 knockout. (ximbio.com)

Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)

Genetic deletion of Dnmt3a in intestinal epithelial cells ( Dnmt3a ΔIEC ) in mice confirms the phenotype of an altered epithelial ultrastructure with shortened apical-junctional complexes, reduced Goblet cell numbers and increased intestinal permeability in the colon in vivo. (nature.com)
The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
Heterozygote knockout phenotype is expected to be similar to the wild-type phenotype. (mmrrc.org)

Cancer susceptibility of mice with a homozygous deletion in the COOH-terminal domain of the Brca2 gene. (jax.org)
Most mice with the whole-body TAZ deletion died before birth, apparently because of skeletal muscle weakness. (ubigene.us)

Capecchi and Smithies independently discovered applications to mouse embryonic stem cells, however the highly conserved mechanisms underlying the DSB repair model, including uniform homologous integration of transformed DNA (gene therapy), were first shown in plasmid experiments by Orr-Weaver, Szostack and Rothstein. (wikipedia.org)
Genetic deficiency induces embryonic lethality in Dnmt1 and Dnmt3b-deleted mice and postnatal lethality in Dnmt3a-knockout mice at ~4 weeks of age 10 . (nature.com)
Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)
A 1997 study of mice grown from embryonic stem cells infected with ROSAβgeo retrovirus showed expression throughout every tissue in the body. (renmab.com)

Genetic testing for deleterious mutations in breast cancer 1, early onset gene ( BRCA1 ) and BRCA2 can provide key information to guide clinical decision making. (jci.org)
In the clinic, genetic testing for BRCA1 and BRCA2 mutations is offered to women in high-risk families and yields one of several possible results. (jci.org)
Approximately 1 in every 500 newborns exhibits a degree of hearing loss, and more than half of cases are associated with genetic mutations [ 1 ]. (biomedcentral.com)

Single guide RNAs were designed to excise Dmd exon 23, with flanking intronic regions repaired by non-homologous end joining. (ox.ac.uk)

Indeed, in DT40 cells, they found an epistatic relationship between Tdp2 and Ku70, a component of the non-homologous end-joining (NHEJ) pathway, in the repair of trapped Top2 covalent complexes. (prolekarniky.cz)

Genetic variants in the DNA methyltransferase 3 A (DNMT3A) locus have been associated with inflammatory bowel disease (IBD). (nature.com)
The Rosa26 locus in the mouse genome is a "safe harbor" which allows researchers to express genes of interest. (renmab.com)
Scientifically referred to as GtROSA26, this locus is found on chromosome 6 of mice. (renmab.com)
Due to its ease of knocking in DNA, the Rosa26 locus on mouse chromosome 6 is very useful for scientists. (renmab.com)

There are several thousand different strains of knockout mice. (wikipedia.org)

In this Application Note, we discuss the benefits and drawbacks of such integration, the use of a "Safe Harbor" for transgene integration in mice and humans, and a demonstration of the ability of the GeneCopoeia genome editing tools for Human AAVS1 and Mouse Rosa26 Safe Harbor to integrate transgenes at a "safe" genomic site. (genecopoeia.com)
The associated genes with stearic acid are Homologous Gene, ACLY gene, Transgenes, FATE1 gene and Alleles. (cmdm.tw)

Here, we review the arrival of genetic screens in malaria parasites to analyse parasite gene function at a genome-scale and their impact on understanding parasite biology. (portlandpress.com)
This work was done in primate parasites, and Kappe's project was to find homologous molecules in rodent malaria parasites. (the-scientist.com)

A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)
Gene targeting allows researchers to design and produce "knockout" lab mice to study how the disabled gene works. (utah.edu)
This strategy should provide new avenues for clinicians to interpret results of genetic testing of BRCA1 variants and for researchers to study the basic molecular mechanisms of BRCA1 function in in vivo model systems. (jci.org)
that brought together many international researchers and highlighted the emerging genetic diversity in CLCN4 patients. (metrionbiosciences.com)
Researchers separated the mice that had the Rosa26 insertion for future study. (renmab.com)
Prior to gene targeting, researchers created transgenic mice. (renmab.com)

In contrast, gene targeting allows scientists to either "knockout" a gene of interest or make an insertion - knock-in - at a specific site in the mouse genome. (renmab.com)

The mutant saul1-1 displays seedling lethality from its autoimmunity, which makes it a suitable background for genetic screens. (ubc.ca)

Prior to this, geneticists used transgenic mouse models to test hypotheses. (renmab.com)
For fast and simple integration of loxP-sites into large vector plasmids at any intended position (for generation of transgenic mouse models). (biosave.com)

For this example, we will take stem cells from a white mouse. (wikipedia.org)
The quest to understand the workings of human cells will not be over until we understand how this genetic blueprint is used to produce a particular set of proteins - the proteome - for each type of cell and how these proteins control the physiology of the cell. (learner.org)
Excellence Professor of Pathology and Laboratory Medicine at the University of North Carolina, Chapel Hill, are being honored for developing gene targeting - the ability to alter particular genes in cultured cells and transfer the targeted genes to laboratory mice. (utah.edu)
In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
Other recent studies verified the presence of PAPP-A mRNA in granulosa cells of humans, monkeys, cattle, mice, and pigs. (bioone.org)
At university, he began a thesis project focusing on how Plasmodium parasites enter mouse cells. (the-scientist.com)
Gene knockouts have been described in (avian) DT40 cells [14] and mouse [15] , confirming etoposide sensitivity of vertebrate cells lacking Tdp2. (prolekarniky.cz)
Gómez-Herreros and colleagues extended the genetic analysis of Tdp2 using the knockouts in DT40 cells and mouse alluded to above. (prolekarniky.cz)
Cas9 nuclease, single guide RNA, and donor DNA were transfected into mouse cells. (eco-vector.com)
An approach to obtaining clones of mouse genetically modified stem cells expressing pathological humanized α-synuclein, has been proposed and implemented. (eco-vector.com)
In this study we developed a functional assay using mouse ES cells to study variants of BRCA1. (jci.org)
In this issue of the JCI , Chang, Sharan, and colleagues describe a novel system to evaluate human BRCA1 alleles for in vivo function using BACs containing human BRCA1 vectors in mouse cells and embryos (see the related article beginning on page 3160). (jci.org)
Such studies examine mice from two zygotes that create animals with different genotypes in their cells. (renmab.com)
Rosa26 mice express β-galactosidase throughout their cells. (renmab.com)

They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)
While AND-34 is widely expressed in wild type mice, histologic analysis of multiple organs in AND-34 −/− mice is unremarkable and analyses of lymphocyte development show no overt changes. (molvis.org)

Clone 126-2F4 was found out carrying the necessary genetic modifications. (eco-vector.com)
For fast knockouts or modifications of genes on the E.coli chromosome. (biosave.com)

Humans share many genes with mice. (wikipedia.org)
Because humans share the vast majority of their genes with mice, gene-targeted mice are used to reproduce diseases that occur in humans. (utah.edu)

The developed cell clone can serve to create a line of genetically modified mice that serve as a test system for pathophysiological and neuropharmacological studies associated with synucleinopathies. (eco-vector.com)
However, in vivo testing of these hypotheses has predominantly been relegated to slow, expensive, and linear generation of genetically modified mice. (mdpi.com)

1997). They found that one particular strain of mice-named ROSAβgeo26-expressed β galactosidase from a randomly inserted transgene at high levels uniformly in nearly all tissues examined. (genecopoeia.com)
Tdp2 was identified by Cortés-Ledesma and colleagues by a genetic screen using expression of a human cDNA library in yeast followed by selection for camptothecin resistance of a yeast strain lacking tdp1 and rad1 [12] . (prolekarniky.cz)

Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
and 4) Expression of a protein from a particular species, such as mice, in an orthologous organism such as humans. (genecopoeia.com)
The demonstration of what could happen was great, and they used mice because it's a more tractable system, but what we cared about were humans. (medscape.com)

Genome editing has many applications, including gene knock out, gene tagging, and correction of genetic defects, to name a few. (genecopoeia.com)
Genome-wide analysis of chromatin states reveals distinct mechanisms of sex-dependent gene regulation in male and female mouse liver. (omicsdi.org)
One limitation of transgenesis is that plasmid DNA randomly integrates into the mouse genome. (renmab.com)
One disadvantage to this method is that the transgene randomly integrates into the mouse genome. (renmab.com)
For comprehensive investigation of the genetic heterogeneity of diseases with a wide range of causative genes, such as hearing loss, and to identify novel candidate genes, WES analysis overcomes the limitations of targeted analysis and is considerably more cost-effective than whole genome sequencing (WGS) analysis. (biomedcentral.com)

In this study, we sought to explore the wide spectrum of genetic heterogeneity associated with hearing loss in Japan, and to discover novel candidate genes associated with hearing loss, using trio analysis of probands and their parents, and four originally developed gene groups ranked by priority (tiers), as a new strategy to filter candidate variants. (biomedcentral.com)

The structures of Tdp2 from C. elegans , zebrafish, and mouse have been determined, indicating an active site that accommodates adducted single-strand DNA [16] , [17] . (prolekarniky.cz)

Although DNMT3A and DNMT3B are highly homologous, their redundancy seems to be limited, and their unique biological function, e.g., specificity for methylation patterns in distinct cell types, is still elusive 9 . (nature.com)
Knockout mice also offer a biological and scientific context in which drugs and other therapies can be developed and tested. (wikipedia.org)
14-3-3 proteins are a family of homologous proteins involved in many biological processes including signaling, neurite outgrowth and ion channel regulation. (fsu.edu)
Our unique approach offers the industry a totally different approach to generating fully human antibodies to important biological targets than Cambridge Antibody Technology's phage display approach or the transgenic mouse approach used by Medarex or Abgenix," says Li Zhu, president and CEO of Genetastix. (indexarticles.com)

These species included dozens of bacteria and other microbes, as well as the model systems: yeast, fruit fly, nematode, and mouse. (learner.org)

Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)

In Chapter 2, we found that when 14-3-3 proteins are inhibited in the 14-3-3 FKO mice this causes dysregulation of NMDA receptors and actin-signaling at the synapse, possibly leading to deficits in synaptic activity and spine formation. (fsu.edu)

Knockout mice also have been used to study and model varieties of cancer, obesity, heart disease and other illnesses. (wral.com)
The mouse model of diabetes-accelerated atherosclerosis was induced by streptozotocin injections and an atherogenic diet. (hindawi.com)
This is due to a paucity of functional annotation from sequence homology, which is compounded by low genetic tractability compared with many model organisms. (portlandpress.com)
In order to investigate the potential associate between 14-3-3 dysregulation and schizophrenia, our lab has created a novel mouse model that addresses the collective function of all 14-3-3 isoforms in the brain. (fsu.edu)
Glutathione transferase P1: an endogenous inhibitor of allergic responses in a mouse model of asthma. (ximbio.com)
Efforts to make a mouse model using traditional methods had been unsuccessful. (ubigene.us)
The rationale for scientists to use Rosa26 and other key benefits to using this type of mouse genetic model will be discussed. (renmab.com)

These transgenic mice express a 14-3-3 peptide inhibitor (YFP-difopein) that antagonizes 14-3-3 binding to its endogenous partners and is thus considered a 14-3-3 functional knockout (FKO). (fsu.edu)

Dnmt3a ΔIEC mice suffer from increased susceptibility to experimental colitis, characterized by reduced epithelial regeneration. (nature.com)

Chromatin state maps were developed to elucidate sex differences in chromatin structure and their impact on sex-differential chromatin accessibility and sex-biased gene expression in mouse liver. (omicsdi.org)

Development of lethal models of Ebola virus disease has been achieved by the serial passage of virus isolates from human cases in mice and guinea pigs. (cdc.gov)

Genetic and pharmacological interventions have successfully extended healthspan and lifespan in animals, but their genetic interventions are not appropriate options for human applications and pharmacological intervention needs more solid clinical evidence. (mdpi.com)
At the same time, after a Cre-dependent knockout activation, it is possible to imitate the pharmacological inhibition of α-synuclein, which is of particular interest for applied research in neuropharmacology. (eco-vector.com)

We found that GGTase-I knockout attenuated diabetes-accelerated atherosclerosis in vivo and suppressed high-glucose-induced VSMC proliferation in vitro. (hindawi.com)

Moreover, after a 16-week duration of diabetes, Pggt1b Δ / Δ mice exhibited lower α -smooth muscle actin ( α -SMA) and nitrotyrosine level, Rac1 activity, p47phox and NOXO1 expression, and phospho-ERK1/2 and phosphor-JNK content than wild-type mice. (hindawi.com)

De Murcia and colleagues provided the first evidence implicating PARP-1 in DNA repair by demonstrating that PARP-1-deficient mice are highly sensitive to γ-irradiation 21 . (nature.com)
Pu, Wang, and colleagues then used gene therapy to replace TAZ, injecting an engineered virus under the skin (in newborn mice) or intravenously (in older mice). (ubigene.us)

For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)
Duchenne muscular dystrophy (DMD) is the most prevalent inherited myopathy affecting children, caused by genetic loss of the gene encoding the dystrophin protein. (ox.ac.uk)
Restoration of dystrophin protein expression was demonstrated by western blot and immunofluorescence staining in mice treated via either intraperitoneal or intravenous routes of delivery. (ox.ac.uk)

WHITE PLAINS, N.Y., May 9, 2005 - For developing an indispensable tool for today's genetic disease research, two scientists have been named co-recipients of this year's March of Dimes Prize in Developmental Biology, to be awarded May 16 in Washington, DC. (utah.edu)

In the study of genomic cis -regulatory elements, generation of mice featuring transgenic reporters or cis -regulatory element knockout remains the standard approach. (mdpi.com)
The ability to follow genes throughout a line allows for a more in-depth study of how genetic code passes down and express themselves over generations. (renmab.com)
Study Homozygotes Resulting From Knock-In While mice with combined genotypes prove useful for study, mice with singular genotypes from knocking in a gene at the Rosa26 site are also beneficial. (renmab.com)
The viability of these mice ensures the longer study of the results of the added gene at the Rosa26 location. (renmab.com)

Here, we analyzed 45 mouse liver RNA sequencing (RNA-Seq) data sets collected under diverse conditions to systematically characterize 4,961 liver lincRNAs, 59% of them novel, with regard to gene structures, species conservation, chromatin accessibility, transcription factor binding, and epigenetic states. (omicsdi.org)

Usually, the new sequence is also given a marker gene , a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (wikipedia.org)

These new combinations of DNA represent genetic variation in offspring, which in turn enables populations to adapt during the course of evolution. (wikipedia.org)

The chimera mice are crossed with a normal wildtype mouse (grey). (wikipedia.org)
Chimera analysis is another application of the Rosa26 mouse. (renmab.com)

This effect is most likely due to disturbance in circuit connections within the prefrontal cortex and hippocampus, as restoring 14-3-3 function in both brain regions was necessary in order to attenuate psychomotor disturbances in the 14-3-3 FKO mice. (fsu.edu)

A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse. (wikipedia.org)

Orthologs for many liver lincRNAs were identified in one or more supraprimates, including two rat lincRNAs showing the same growth hormone-regulated, sex-biased expression as their mouse counterparts. (omicsdi.org)

TAZ gene therapy also prevented cardiac dysfunction and scarring when given to newborn mice, and reversed established cardiac dysfunction in older mice -- whether the mice had whole-body or heart-only TAZ deletions. (ubigene.us)
This mutant mouse exhibits plaque and tangle pathology associated with synaptic dysfunction, traits similar to those observed in Alzheimer's disease patients. (mmrrc.org)

These wrongly attempted fixes can lead to genetic abnormalities that could increase the risk of cancer. (the-scientist.com)
Enlarged ventricles and septal abnormalities were also features of the mutant mouse brain. (jneurosci.org)

Examples of research in which knockout mice have been useful include studying and modeling different kinds of cancer , obesity , heart disease , diabetes , arthritis , substance abuse , anxiety , aging and Parkinson's disease . (wikipedia.org)

reported that GGTase-I deficiency in mouse macrophages slowed down the progression of atherosclerosis [ 9 ]. (hindawi.com)

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