Genetic sequence analysis rna. Medical search. Frequent questions

ASAP-seq uses a bridging approach that repurposes antibody:oligonucleotide conjugates designed for existing technologies that pair protein measurements with single-cell RNA sequencing. (nature.com)
For single-cell RNA and protein data analysis. (biolegend.com)
The researchers, led by PhD candidate Sara Bottes and postdocs Baptiste Jaeger and Gregor Pilz, employed state-of-the-art microscopy and genetic analyses (using single-cell RNA sequencing) of stem cells and their daughter cells to analyze the formation of new nerve cells. (myscience.ch)
The researchers also used single-cell RNA sequencing of stem cells and their daughter cells to demonstrate that stem cells with different division behavior (few cell divisions as opposed to long-lasting stem cell activity) can be differentiated on the basis of their molecular composition and gene expression profiles. (myscience.ch)
Combining two modern methods - two-photon microscopy and single-cell RNA sequencing - has enabled us to identify precisely the stem cells that can divide over the course of months,' explains Jessberger. (myscience.ch)
Single-cell RNA sequencing gives us our first insights into what genes are important in terms of the division behavior of individual cells. (myscience.ch)
Jessberger sums up the next research objectives: "Imaging and single-cell RNA sequencing have given us completely new insights that we will use in the future to systematically regulate the activity of certain genes in the future. (myscience.ch)
While many flavors of single-cell sequencing have been developed, single-cell RNA sequencing (scRNA-seq) is currently the most prolific form in published literature. (techscience.com)

The 16S and 12S ribosomal RNA genes are 1567 and 921 nucleotides in length. (wikipedia.org)
The whole mitogenome of O. conispadiceus is 16,027 basepairs (bp) in length with a base composition of 41.4% A, 34.8% T, 16.1% C, 7.7% G and contains 13 protein-coding genes, 2 ribosomal RNA genes, 22 transfer RNA genes, and a major non-coding region (MNR). (tonmo.com)
In addition, mitochondrial DNA (mtDNA) encodes 2 ribosomal RNA genes and 22 transfer RNA (tRNA) genes necessary for the intramitochondrial synthesis of these 13 polypeptides. (medscape.com)

Genomic and transcriptomic sequencing analyses were performed with phenotypic validation to investigate its adaptation in SARS-CoV-2 infected environment. (frontiersin.org)
For the genomic information, the researchers ran single nucleotide polymorphisms (SNP) microarray that interrogated 4,327,108 genetic variants selected from the International HapMap and 1,000 Genomes Projects and targeted genetic variation down to 1% minor allele frequency (MAF). (news-medical.net)
Genomic analyses clustered only one couple together per copy number variation (CNVs). (news-medical.net)
AAV type 2 wild-type and vector-mediated genomic integration profiles in human diploid fibroblasts analyzed by 3rd generation PacBio DNA sequencing. (genomeweb.com)
This bi-genomic division is accompanied by profoundly different transcription regulatory system: whereas nDNA-encoded genes are transcribed individually by RNA polymerase 2 and the general nuclear transcription machinery, mtDNA transcription is long known to be regulated mainly by a dedicated RNA polymerase (POLRMT) and mtDNA-specific transcription factors (TFAM and TFB2) ( Shutt and Shadel 2010 ). (biorxiv.org)
AMD technology uses genomic sequencing, high-performance computing, and epidemiology to study pathogens and improve disease detection. (cdc.gov)

In the post-course assessment, nurses are rated on metrics including their understanding of the relationship of genetics and genomics to health, prevention, screening, and diagnostics, and the ability to put together a family health history and patient genetic pedigree from this family history information. (genomeweb.com)
In genetics and genomics research, gene expression refers to the process by which information encoded in a gene's DNA sequence is converted into functional proteins or RNA molecules. (pharmiweb.com)
Next-Generation Sequencing (NGS) is a technology that enables rapid and accurate sequencing of DNA or RNA, which has revolutionized the field of genomics. (learntoupgrade.com)
By learning NGS, you will develop skills in bioinformatics, data analysis, and interpretation, which are essential for modern genomics research. (learntoupgrade.com)
Anyone who is interested in genomics research, including scientists, clinicians, and students, can learn Next-Generation Sequencing (NGS) course. (learntoupgrade.com)

As with other genetic sequences, the majority of genes that produce proteins began with the ATG start codon. (wikipedia.org)
While the genome is the DNA content comprising the genetic information essential for life, the transcriptome includes only the information on genes that are expressed, while the proteome is the totality of proteins expressed at a given time and under specific conditions. (eurekalert.org)
Simultaneously detect surface proteins and RNA with our TotalSeq antibodies, panels, and hashtags which integrate seamlessly into existing single-cell workflows. (biolegend.com)
The new method analyzes various gene isoforms - combinations of genetic sequences that can produce an enormous variety of RNAs and proteins from a single gene - using data from RNA molecules in cancer specimens. (uclahealth.org)
Phylogenetic analysis of CRISPR-associated (Cas) proteins suggests there are at least seven distinct versions of this immune system. (berkeley.edu)
To explore this diversity, we utilize metagenomic analysis of microbial communities across the tree of life to discover novel proteins for efficient genome editing. (berkeley.edu)
To discover the molecular programs controlling microglial and macrophage polarization by blood proteins, we developed an unbiased blood-innate immunity multiomic and genetic loss-of-function pipeline consisting of deep sequencing of blood-induced transcriptomes, functional single-cell and oxidative stress transcriptomics, global phosphoproteomics and integration with innate immune signatures from AD and MS models (Extended Data Fig. 1 ). (nature.com)
Recent multi-omics studies have revealed the heterogeneity and complexity of tumor features such as their genetic mutations, transcriptome, proteins, and signaling pathways. (rsc.org)
RNA-binding proteins contribute to specificity by interacting with both Ccr4-Not and target mRNAs, but this is not fully understood. (elifesciences.org)
Known as "destabilising motifs", these sequences attract the attention of a group of proteins called Ccr4-Not. (elifesciences.org)

Subsequent mutation analysis of the factor H gene has revealed two mutations in patients with HUS. (nih.gov)
Foster City, Calif., December 1, 2021 - Gilead has conducted an analysis of genetic information currently available for the Omicron variant and found no additional prevalent mutations in the viral RNA polymerase compared to previous SARS-CoV-2 variants. (gilead.com)
Initial genetic analysis of more than 200 available sequences of the Omicron variant isolates, including those from South Africa, Asia and Europe, has shown that no new mutations are present in the Omicron variant that are expected to alter the SARS-CoV-2 viral RNA polymerase compared to previous variants. (gilead.com)
Personalized medicine's foundation rests on the use of molecular technologies, which are being used to identify genetic mutations, polymorphisms, and variants that can be associated with an individual's genetic make up, revealing risk factors and predictive data. (medscimonit.com)
NGS technology can help identify genetic mutations or variants associated with specific diseases, which can aid in personalized medicine approaches. (learntoupgrade.com)
As each mitochondrion contains 2-10 copies of mtDNA and each cell contains hundreds to thousands copies, the presence of heterogeneous mtDNA sequences, due to different mutations in different copies, results in a condition called heteroplasmy. (northwestern.edu)
Peu de renseignements sont disponibles sur les mutations des virus saisonniers de la grippe A(H1N1)pdm09 et H3N2 en Jordanie. (who.int)
Les mutations individuelles sont décrites en détail. (who.int)
We are currently using AMD techniques to sequence all clinical specimens that come into the CDC to improve our ability to find and track mutations that may be of concern. (cdc.gov)
Whole genome sequencing can also provide insights into the specific genes or mutations that may be linked to antibiotic resistance. (folkhalsomyndigheten.se)

In collaboration with the laboratory of David Savage , we have also developed a series of high-throughput mutant library construction, screening, and sequencing pipelines to probe CRISPR-Cas protein function and to isolate engineered variants with novel properties. (berkeley.edu)
Assessing the impact of water treatment on bacterial biofilms in drinking water distribution systems using high-throughput DNA sequencing. (genomeweb.com)
MToolBox: a highly automated pipeline for heteroplasmy annotation and prioritization analysis of human mitochondrial variants in high-throughput sequencing. (genomeweb.com)
This review provides an overview of the basic principles of high-throughput microbiome sequencing techniques, current literature on sputum microbiome profiling in COPD, and a discussion of the associated limitations and future perspectives. (bmj.com)
Therefore, researchers in these fields can benefit from learning NGS to generate high-throughput sequencing data that can be analyzed to obtain insights into complex biological systems. (learntoupgrade.com)
It offers high quality products and services for molecular biologists who require DNA/RNA oligonucleotides, robust sequencing - such as high throughput Sanger sequencing - and analysis solutions, as well as outsourcing of contract research projects. (integra-biosciences.com)

Prokaryotes have evolved a nucleic acid-based immune system that shares some functional similarities with RNA interference in eukaryotes. (berkeley.edu)
Microsynth, founded in 1989, is a leading European company in nucleic acid synthesis, analysis and sequencing. (integra-biosciences.com)
Microsynth is using INTEGRA's MINI 96 and VIAFLO 96 handheld electronic pipettes in every step of sample preparation, in workflows ranging from nucleic acid isolation to next generation sequencing (NGS) and different types of PCR. (integra-biosciences.com)
All nucleic acid in the clinical sample is sequenced and the resulting data is analysed by experienced bioinformaticians. (folkhalsomyndigheten.se)

Clot RNAs from the two patients were subjected to reverse transcription-polymerase chain reaction (PCR) amplification and sequencing. (cdc.gov)
Due to the similarities in the viral RNA polymerase, these laboratory findings suggest that Veklury will also continue to be active against the Omicron variant and Gilead will conduct laboratory testing to confirm this analysis. (gilead.com)
To date, no major genetic changes have been identified in any of the known SARS-CoV-2 variants of concern and variants of interest that would significantly alter the viral RNA polymerase targeted by Veklury. (gilead.com)
Helicase, RNA-dependent RNA polymerase (RdRp) and nucleocapsid or envelope regions were targeted for amplification and sequencing. (blogspot.com)
The find, represented by a phylogenetic tree using on a 181nt RNA sequence fragment from the RNA-dependent RNA polymerase gene (100% identical to a sequence from the index case in Bisha, betaCoV 2c EMC/2012 over this region), secures bats as the/a primary animal source. (blogspot.com)
One of the main reasons that the virus is challenging, is due to its' RNA polymerase. (cdc.gov)

RNA-sequencing (RNA-seq) is a powerful technique for the identification of genetic variants that affect gene-expression levels, either through expression quantitative trait locus (eQTL) mapping or through allele-specific expression (ASE) analysis. (biomedcentral.com)
We also derived genotypes using the RNA-seq reads and used imputation to infer non-coding variants. (biomedcentral.com)
eQTL mapping on individual tissues revealed that a limited number of samples already suffice to identify tissue-specific eQTLs for known disease-associated genetic variants. (biomedcentral.com)
Given the exponential growth of the number of publicly available RNA-seq samples, we expect this approach will become especially relevant for studying the effects of tissue-specific and rare pathogenic genetic variants to aid clinical interpretation of exome and genome sequencing. (biomedcentral.com)
Ideally, eQTL data on many tissues and many different samples should be available since this would permit eQTL mapping and ASE analyses on rare and low-frequency variants within different cell types. (biomedcentral.com)
In addition to genetic analyses, Gilead continues to experimentally evaluate the activity of Veklury against identified SARS-CoV-2 variants through in vitro antiviral testing. (gilead.com)
We performed a genome-wide association study (GWAS) and exome-sequencing analysis to identify common and rare genetic variants associated with plasma myeloperoxidase (MPO)-DNA complex levels, a biomarker for NETs, in the population-based Rotterdam Study cohort. (frontiersin.org)
We additionally performed exome-sequencing analysis in 960 individuals to investigate rare variants in candidate genes. (frontiersin.org)
Genome-wide association studies (GWAS) and exome-sequencing analysis have been successfully implemented as approaches to identify genetic variants associated with disease susceptibility. (frontiersin.org)
The assessment of genetic variants in association with NETs might help to elucidate potential molecular mechanisms intervening in their formation and their downstream effect on other pathways. (frontiersin.org)
Here, we are the first to apply these approaches to ascertain common and rare genetic variants associated with NETs using data from a population-based cohort study. (frontiersin.org)
We performed additional in silico analyses to identify more evidence for the associated variants and genes in relation to the plasma MPO-DNA complex levels. (frontiersin.org)
KBG syndrome is a rare neurodevelopmental disorder caused by heterozygous variants in the ANKRD11 gene and is one of the most prevalent genetic syndromes. (bmj.com)
Authors are encouraged to attempt these aneuploid analyses when reporting scRNA-seq data, much like copy-number variants are commonly reported in bulk genome sequencing data. (techscience.com)

Exploring genetic variation in the tomato ( Solanum section Lycopersicon) clade by whole-genome sequencing. (genomeweb.com)
Variant detection sensitivity and biases in whole genome and exome sequencing. (genomeweb.com)
Whole-genome sequence analysis reveals differences in population management and selection of European low-input pig breeds. (genomeweb.com)
Some patients with suspected autosomal recessive disorders were recruited for whole genome sequencing (WGS) through the 100,000 Genomes Project after only one causative variant was detected. (bmj.com)
For contact tracing or inquiries related to antibiotic resistance, whole genome sequencing (WGS) of bacterial isolates is offered. (folkhalsomyndigheten.se)
It is a platform and database for the automated analysis of whole genome sequencing data. (folkhalsomyndigheten.se)

In addition, it may allow scientists to analyze people's genetic sequences and determine which are lethal and which are harmless. (uclahealth.org)
This suggests that Veklury will continue to be active against the Omicron variant and Gilead will continue to analyze additional genetic sequences of the Omicron variant as they become available. (gilead.com)
It may analyze and measure the specific makeup of a certain gene, in order to help better identify the particular genetic makeup that might be shared with others or signal a possible health concern. (healthline.com)
In addition, the course will teach students how to analyze NGS data using various bioinformatics tools and software, such as alignment, variant calling, and gene expression analysis. (learntoupgrade.com)
CDC is using next-generation gene sequencing tools to analyze flu viruses as part of CDC's Advanced Molecular Detection (AMD) initiative. (cdc.gov)

That process, called RNA sequencing, or RNA-seq, reveals the presence and quantity of RNA molecules in a biological sample. (uclahealth.org)
RNA molecules are uniquely capable of encoding and controlling the expression of genetic information, often as a consequence of their three-dimensional structures. (berkeley.edu)
This enzyme is involved in producing pyrimidines, which are building blocks of DNA, its chemical cousin RNA, and molecules such as ATP and GTP that serve as energy sources in the cell. (medlineplus.gov)

To simplify data analysis, we provide Multiomics Analysis Software (MAS), a free cloud-based program accessible to all users irrespective of their bioinformatics background. (biolegend.com)
Our facility staff have expertise in most aspects of DNA sequence analyses and can offer help and advice on experimental design, library constructions, analyses and bioinformatics and provides assistance to both beginners and experienced users. (lu.se)
The course typically covers topics such as sequencing platforms, library preparation, quality control, bioinformatics analysis, and interpretation of NGS data. (learntoupgrade.com)
Standard mtDNA-seq bioinformatics service includes sequencing data QC, alignment to the reference mitochondrial genome, and variant analysis. (northwestern.edu)

This study characterized the canopy-layer specific transcriptome of five sorghum genotypes using RNA sequencing. (biomedcentral.com)
Here, we established an unbiased blood-innate immunity multiomic and genetic loss-of-function pipeline to define the transcriptome and global phosphoproteome of blood-induced innate immune polarization and its role in microglia neurotoxicity. (nature.com)
Evidence from genome-wide association studies highlight the importance of genetic and epigenetic factors in the development of obesity and how they in turn affect the transcriptome, metabolites, microbiomes, and proteomes. (springermedizin.at)
This includes trimming adapter sequences, filtering low-quality reads, and aligning the reads to a reference genome or transcriptome. (pharmiweb.com)
In this study, we present integrated quantitative proteome, transcriptome, and methylome analyses of hematopoietic stem cells (HSCs) and four multipotent progenitor (MPP) populations. (lu.se)

Given increasing numbers of RNA-seq samples in the public domain, we here studied to what extent eQTLs and ASE effects can be identified when using public RNA-seq data while deriving the genotypes from the RNA-sequencing reads themselves. (biomedcentral.com)
In a joint analysis on the 1,262 samples with high quality genotypes, we identified cis- eQTLs effects for 8,034 unique genes (at a false discovery rate ≤0.05). (biomedcentral.com)
By deriving and imputing genotypes from RNA-seq data, it is possible to identify both eQTLs and ASE effects. (biomedcentral.com)
HDV genotypes were characterized by Next-generation sequencing (NGS), followed by phylogenetic analyses. (researchsquare.com)
Phylogenetic analyses indicated that HDV-2 genotype being the predominant genotype, other HDV genotypes were not observed. (researchsquare.com)
Moreover, Next-generation sequencing (NGS) technologies provide a rapid, precise method for generating HDV genomes to define infecting genotypes. (researchsquare.com)

SURVIV (for "survival analysis of mRNA isoform variation") is the first statistical method for conducting survival analysis on isoforms using RNA-seq data, said senior author Yi Xing , UCLA associate professor of microbiology, immunology and molecular genetics. (uclahealth.org)
Our finding suggests that isoform ratios provide a more robust molecular signature of cancer patients in large-scale RNA-seq datasets," he said. (uclahealth.org)
Our results uncover the structural basis for foreign DNA capture and the mechanism by which Cas1-Cas2 functions as a molecular ruler to dictate the sequence architecture of CRISPR loci. (berkeley.edu)
Pathway analysis of the identified genes showed their involvement in cellular development, molecular transport, RNA trafficking, cell-to-cell signaling and interaction, cellular growth and proliferation. (frontiersin.org)
By subjecting DEGs to gene ontology enrichment analysis, pathway analysis, or functional annotation, we gain insights into the biological processes, molecular functions, and pathways associated with the observed gene expression changes. (pharmiweb.com)
The first DNA sequence of a cephalopod was published in 1983 and the first molecular paper focusing on cephalopods was published in 1994. (tonmo.com)

Genetic and phylogenetic analyses revealed that this Sanmenxia H5N1 virus was a novel reassortant, possessing a Clade 2.3.2.1c HA gene and a H9N2-derived PB2 gene. (nature.com)

Participants on various protocols can have a box to check yes or no to that so that they are all aware of whether they're going to have exome sequencing as part of the protocol," Wallen explained. (genomeweb.com)
In this first GWAS and exome-sequencing analysis of NETs levels, we found several genes that were associated with NETs. (frontiersin.org)
Can I use NGS data from other projects, such as exome-seq, RNA-seq, ChIP-seq, or ATAC-seq, for mtDNA sequence analysis? (northwestern.edu)

We describe the first report of RNA sequencing of 5' capped (Pol II) RNAs isolated from acutely hepatitis C virus (HCV) infected Huh 7.5 cells that provides a general approach to identifying differentially expressed annotated and unannotated genes that participate in viral-host interactions. (mdpi.com)
The HDV virions possess an outer layer containing hepatitis B surface antigens (HBsAg) and host lipid surrounding the inner nucleocapsid that consists of viral RNA. (researchsquare.com)
Profs Ziad Memish and Ian Lipkin, and a team of collaborators including researchers from the EcoHelath Alliance , have published, in Emerging Infectious Diseases , their discovery of viral sequences in the faecal pellet of an Egyptian tomb bat. (blogspot.com)
8 however, laboratory-based surveillance and detailed analyses of viral transmission patterns have not been conducted previously. (who.int)

Epigenetic analysis revealed a specific N 6 -methyl adenine (m 6 A) methylating pattern including methylation of alginate, flagellar and quorum sensing associated genes. (frontiersin.org)
For epigenetic analyses, the researchers used a DNA methylation microarray that evaluated over 0.85 million 5'-cytosine-phosphate-guanine-3' (CpG) sites. (news-medical.net)
Single-cell genome-wide bisulfite sequencing for assessing epigenetic heterogeneity. (genomeweb.com)

The method uses an internal control sequence (spike) which is amplified and sequenced simultaneously with the sample to facilitate the assessment of the bacterial content and to detect amplification-inhibiting factors in the sample that may lead to a negative result. (folkhalsomyndigheten.se)

When a cell needs to make a particular protein, it first copies the instructions from the matching gene into a molecule known as a messenger RNA (or an mRNA for short). (elifesciences.org)
The CDC's move came the day after the US Food and Drug Administration (FDA) approved Moderna's and Pfizer-BioNTech's updated COVID-19 messenger RNA (mRNA) vaccines for people 12 years or older and authorized the shots for emergency use in children 6 months through 11 years of age. (cdc.gov)

The main focus of the work was to understand which factors shape microbial community composition and diversity in response to different cropping systems, namely monoculture and intercropping, in winter wheat (Triticum aestivum L.) and winter faba bean (Vicia faba L.) using Illumina (MiSeq) sequencing. (uni-goettingen.de)
Culture-independent microbial sequencing techniques have revealed that the respiratory tract harbours a complex microbiome not detectable by conventional culturing methods. (bmj.com)
Through a clever multi-omics design, the authors were able to use three major types of integrated analyses to identify differences in encoded and expressed microbial functions that were involved in the BPA-degrading microbial community. (rsc.org)
The basis of the GENSAM project is the joint national management of clinical microbial sequencing data. (folkhalsomyndigheten.se)

ABSTRACT Understanding the genetic evolution of A(H1N1)pdm09 and H3N2 viruses can help better select strains to be included in the annual influenza vaccine. (who.int)

Even though these data originated from many different laboratories, samples reflecting the same cell type clustered together, suggesting that technical biases due to different sequencing protocols are limited. (biomedcentral.com)
Fortunately, the raw data of many RNA-seq experiments are being deposited in public databases, and the number of available human RNA-seq samples is growing exponentially, for example, in the European Nucleotide Archive (ENA) (Figure 1 a). (biomedcentral.com)
5.2 Genetic sequence data. (who.int)
A new method developed by UCLA scientists could eventually lead to a way to do just that, using data about patients' genetic sequences to produce more reliable projections for survival time and how they might respond to possible treatments. (uclahealth.org)
However, given the mass of data emerging from this analysis, and commonalities they reveal between various phenomena/disorders, achieving 100% certainty may not be that easy. (medscimonit.com)
A focused gene signature that predicted G1/G2 tumors from RNA-seq data was developed and validated within an independent cohort of 82 osteosarcomas. (nih.gov)
The lectures will give an overview of the different forms and the importance of DNA and RNA, modern trends in the analysis of gene data, the use of the results for biology, medicine, social and engineering sciences. (leibniz-institut.de)
SpliceNet: recovering splicing isoform-specific differential gene networks from RNA-seq data of normal and diseased samples. (genomeweb.com)
Raw RNA-seq data contains a wealth of information but requires preprocessing steps to ensure accurate and reliable results. (pharmiweb.com)
Psomagen, Inc., a sequencing and data analysis firm under Korean parent company Macrogen, is readying a revolutionary self test kit on Covid-19 that can save visits to test centers for rollout within the year. (daum.net)
Single-cell sequencing data has transformed the understanding of biological heterogeneity. (techscience.com)
Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. (techscience.com)
In this viewpoint, we direct investigators to select scRNA-seq CNA data analysis tools. (techscience.com)
Intuitive "eyeball" calls are uncommon in bulk RNA-seq or DNA-seq determination of aneuploidy and CNAs, so it is unsurprising that a simple stacking of read counts is insufficient for quality inference of aneuploidy in scRNA-seq data. (techscience.com)
Specialized software has thus been developed to appropriately handle RNA-seq data in the context of CNA determination. (techscience.com)
Advanced research skills: NGS technology generates large amounts of data that require advanced computational skills for analysis. (learntoupgrade.com)
Data analysis is provided upon request. (northwestern.edu)
Raw data and analysis results are usually returned to user via OneDrive . (northwestern.edu)
We report haplotyping data for 17 starch biosynthesis/degradation genes demonstrating the broad diversity among H. spontaneum accessions and H. vulgare landraces but rather limited genetic variance in the current elite breeding germplasm by fixing certain haplotypes. (biomedcentral.com)
We have RNA-sequencing data for islets from more than 200 donors, where 35 are diagnosed type 2 diabetics. (lu.se)
Pipelines are series-connected data processes that utilise software tools to structure, process, and interpret genetic information. (folkhalsomyndigheten.se)

We used histologic, immunofluorescence, RNA sequencing, and metabolic assays to characterize kidneys from these mice and controls during development and up to postnatal day 21. (lww.com)
RNA-targeting CRISPR-Cas systems - We use biochemical activity assays in conjunction with structural techniques and sequencing to investigate RNA-targeting CRISPR systems, such as the Type VI effector, Cas13a. (berkeley.edu)

Long-range PCR in next-generation sequencing: comparison of six enzymes and evaluation on the MiSeq sequencer. (genomeweb.com)

Resolve complex genetic questions by combining single-cell DNA analysis with protein detection using TotalSeq-D reagents and the Mission Bio Tapestri platform. (biolegend.com)
This recognition comes as ultra-deep sequencing technologies coupled with error-reducing sequencing techniques have enabled the detection of mutant cells occupying less than one percent of an examined tissue sample ( Martincorena, 2019 ). (techscience.com)
Analysis of non-human organisms enabled de novo mtDNA sequence assembly, as well as detection of previously unknown mtDNA TIS, pausing, and transcription termination sites with unprecedented accuracy. (biorxiv.org)
In contrast to metagenomic detection of unknown pathogens which sequences all available genetic material, 16S sequencing specifically targets the small ribosomal subunit. (folkhalsomyndigheten.se)

Custom code to reproduce all analyses and figures is available at https://github.com/caleblareau/asap_reproducibility . (nature.com)

Our BEN-seq workflow, developed in collaboration with Illumina, stains cells in suspension with TotalSeq antibodies prior to bulk RNA sequencing. (biolegend.com)
Now, the NIH Clinical Center is looking to automate development of these genetic pedigrees and embed the pedigrees into the electronic medical record workflow. (genomeweb.com)

This analysis enables us to pinpoint genes that play pivotal roles in phenotypic variation, disease development, or response to treatments. (pharmiweb.com)
Sequence variations between different bacteria provide a unique identity, and the analysis of these sequence enables taxonomic placement. (folkhalsomyndigheten.se)
In epidemiological contact tracing, this technology enables the mapping of infection spread and identification of sources of infection through the comparison of genetic sequences. (folkhalsomyndigheten.se)

We reasoned that transcriptomic studies should overcome this genetic complexity. (nih.gov)

phylogenetic trees (maximum-likelihood and Bayesian methods) built using these cytochrome oxidase subunit I sequences and others available from GenBank show some support for the subfamilies Cranchiinae and Taoniinae, and that within the latter, the hooked taxa group together. (tonmo.com)
A Next-Generation Sequencing course provides an overview of the basic principles, methods, and applications of NGS technology. (learntoupgrade.com)
Metagenomic sequencing is offered in cases where infection is suspected but other methods have failed to identify a pathogen. (folkhalsomyndigheten.se)

Viruses were characterised by their antigenic, genetic and antiviral drug resistance properties. (health.gov.au)
Results for A(H3N2) and B/Yamagata viruses suggested that circulating viruses of this subtype and lineage, respectively, had undergone antigenic and/or genetic changes, consistent with the decision by WHO to change recommended strains for the 2015 Southern Hemisphere vaccine. (health.gov.au)
Sanmenxia Clade 2.3.2.1c-like H5N1 viruses possess the closest genetic identity to A/Alberta/01/2014 (H5N1), which recently caused a fatal respiratory infection in Canada with signs of meningoencephalitis, a highly unusual symptom with influenza infections in humans. (nature.com)
The Qinghai-like Clade 2.2 virus was found to possess a high genetic relationship with viruses isolated from other countries on the migratory flyway of wild birds 4 , suggesting that the migration of wild birds played an important role in circulating H5N1 HPAIV viruses between the different avian populations. (nature.com)
There is little information on their evolution in Jordan so this study investigated the genetic and antigenic variability of A(H1N1)pdm09 and H3N2 viruses in Jordan by performing phylogenetic and genetic analyses of the HA and NA genes of A(H1N1)pdm09 and H3N2 viruses between 2011 and 2013. (who.int)
The full HA and NA genes of 16 H1N1-positive samples obtained in our study and 21 published HA sequences and 20 published NA sequences from Jordanian viruses that were available on online gene databases were analysed. (who.int)
CDC is using this Next Generation-Sequencing (NGS) technology to monitor genetic changes in influenza viruses in order to better understand and improve the effectiveness of influenza vaccines. (cdc.gov)
Scientists are looking at the genetic sequences of 10 generations of H3N2 flu viruses as they grow and evolve in eggs. (cdc.gov)
CDC will test all of the viruses to find out what genetic changes cause a good immune response and good growth in eggs. (cdc.gov)
Once the "good" genetic changes are identified, CDC will then synthesize H3N2 viruses with those properties that can be used to make vaccine that offers better protection against H3N2 flu infection. (cdc.gov)

People often turn to genetic testing to investigate possible health conditions that run in families or even explore their own family history and heritage. (healthline.com)

Additionally, the genetic architecture of leaf angle across canopy layers remains to be elucidated. (biomedcentral.com)

Because of the increased awareness on the importance of metabolism and bioenergetics in a wide variety of human diseases, more and more mitochondrial DNA (mtDNA) studies are performed to study the link between mtDNA sequence variation and disease development. (northwestern.edu)
NUMTs are a result of insertion of mtDNA sequence into the nuclear genome. (northwestern.edu)

We downloaded the raw reads for all available human RNA-seq datasets. (biomedcentral.com)

Anti-HDV seropositive specimens still need to be tested for serum HDV RNA. (researchsquare.com)

The heterogeneity of the samples, special buffer conditions and liquid properties in DNA and RNA isolation workflows are not always compatible with liquid handling platforms. (integra-biosciences.com)

Moreover, X-ray absorption fine structure (XAFS) analysis reveals the interaction between P and Cd(II), which is further verified via density functional theory (DFT) calculation with the new hybrid peaks resulting from the splitting peak of P atoms coupled with the orbital energy level of Cd(II). (bvsalud.org)

To this end RNA-sequencing will be performed to study differences in allele specific expression and splice isoform pattern between different human tissues. (lu.se)

We employ traditional biochemistry, combined with sequencing, live-cell imaging, and structural biology approaches, to uncover the fundamental mechanisms driving the CRISPR effectors now being developed as genome editing and diagnostic tools. (berkeley.edu)
RNA sequencing (RNA-seq) of 79 osteosarcoma diagnostic biopsies identified stable independent components that recapitulate the tumor and microenvironment cell composition. (nih.gov)
Clinical laboratory diagnostic tests can include tests that, for example, predict the risk associated with one or more genetic variations. (cms.gov)
In addition, in vitro companion diagnostic laboratory tests provide a report of test results of genetic variations and are essential for the safe and effective use of a corresponding therapeutic product. (cms.gov)
Next Generation Sequencing (NGS) is one technique that can measure one or more genetic variations as a laboratory diagnostic test, such as when used as a companion in vitro diagnostic test. (cms.gov)
Medicare Administrative Contractors (MACs) may determine coverage of diagnostic lab tests using NGS for RNA sequencing and protein analysis. (cms.gov)

On the other hand, eQTL analyses can be used for any genetic variant, but typically require the use of dozens of samples in order to have sufficient individuals in the different genotype classes. (biomedcentral.com)
This suggests that Veklury® (remdesivir) will continue to be active against the Omicron variant and Gilead will conduct laboratory testing to confirm this analysis. (gilead.com)
In one example, we utilized domain insertion profiling with sequencing (DIP-seq) to identify an allosteric Cas9 switch, yielding an optimized Cas9 variant that is activated by the small molecule 4-hydroxytamoxifen. (berkeley.edu)

With RNA-seq , we can capture and quantify the abundance of transcripts, revealing the dynamic landscape of gene expression within a cell or tissue. (pharmiweb.com)
Aneuploidy, the loss or gain of chromosome arms, appears less prevalent in normal tissue in these clonal mutant next-generation sequencing analyses. (techscience.com)
Broaden research scope: NGS technology allows researchers to sequence DNA or RNA from any organism or tissue type, which can broaden the scope of your research. (learntoupgrade.com)

Sequence analysis of the haemagglutinin gene was performed in 64 selected influenza B isolates. (who.int)

Unlocking the secondary gene-pool of barley with next-generation sequencing. (genomeweb.com)
Stepwise threshold clustering: a new method for genotyping MHC loci using next-generation sequencing technology. (genomeweb.com)
Fast and cost-effective genetic mapping in apple using next-generation sequencing. (genomeweb.com)
If so, those genes known to be associated with specific cancer or syndrome predisposition are analysed by a state-of-the-art technology known as next-generation sequencing (NGS). (bmj.com)
With the advancement of next-generation sequencing and mass spectrometry, there is a growing need for the ability to merge biological features in order to study a system as a whole. (rsc.org)
One unit for Next-Generation Sequencing (NGS) and a second unit for more traditional Sanger DNA sequencing. (lu.se)
Overall, a Next-Generation Sequencing course is essential for anyone interested in using NGS technology for their research, whether in academia or industry. (learntoupgrade.com)
The Public Health Agency's Next Generation Sequencing (NGS) platform performs massive parallel sequencing of DNA and RNA. (folkhalsomyndigheten.se)

Their focus is creating pipelines to enhance and automate analyses. (folkhalsomyndigheten.se)

Students will learn about the different sequencing technologies available, such as Illumina, PacBio, Oxford Nanopore, and their respective advantages and disadvantages. (learntoupgrade.com)

For microbiome analysis, they performed ribosomal ribonucleic acid (RNA) direct sequencing. (news-medical.net)
Psomagen plans to launch a microbiome analysis service in the second half of this year, while developing a clinical diagnosis solution for hospitals based on its proprietary genome analysis technology. (daum.net)

The mitochondrial genome is an independent genetic system in each eukaryotic cell outside the nuclear genome. (northwestern.edu)
The mitochondrial genome encodes the genetic information for the 13 polypeptide subunits essential for the process of oxidative phosphorylation. (medscape.com)

Seven of the 13 White look-alike doubles did not cluster genetically, indicating alternative purposes for shared genetic variation between look-alike pairs. (news-medical.net)
This study is based on the characterization of genetic variation in barley, with a view to enhancing its response to terminal drought stress. (biomedcentral.com)
Here we present a SNP-based genetic map of barley, specifically focussing on nucleotide variation in ESTs demonstrated to be involved in the response of barley to drought stress occurring at early vegetative stages, during anthesis and the grain filling process. (biomedcentral.com)

Here we present ATAC with select antigen profiling by sequencing (ASAP-seq), a tool to simultaneously profile accessible chromatin and protein levels. (nature.com)
Using a two-color assay to simultaneously monitor poly(A) tail removal from different RNAs, we demonstrate that Puf3 can distinguish between RNAs of very similar sequence. (elifesciences.org)

in Jordan, we performed a phylogenetic analyser (Applied Biosystems, Foster analysis and genetic analysis of the HA City, CA, USA). (who.int)

For the most part, these technologies employ microarray and RNA-Seq methodologies, which examine large numbers of gene expressions at a time, providing clustering and patterns of this expression. (medscimonit.com)
Dr Sergey Yakushev, Head of Genetic Analysis at Microsynth, explained: "My department focuses on genetic analysis, using a range of methodologies - such as conventional PCR, sequencing, and DNA and RNA isolation - which require multiple liquid handling steps during sample preparation and analysis. (integra-biosciences.com)

HDV-specific RT-nested PCR was used to amplify HDV RNA. (researchsquare.com)
Does the long-range PCR performed to enrich mtDNA from total gDNA amplify NUMTs (nuclear mitochondrial DNA sequences)? (northwestern.edu)

However, its characteristic clinical feature and undetermined genetic feature suggests that MNPKL leaves open the possibility of a new independent disease concept. (confex.com)
Here, we report clinical features and genetic alterations in patients with MNKPL. (confex.com)
This article will describe the clinical and research purposes of genetic testing, the health conditions it may help detect, and what you may want to consider when talking with your healthcare team about this type of testing. (healthline.com)
Is there a difference between clinical and research genetic testing? (healthline.com)
Clinical genetic testing aims to find out about any likelihood of an inherited genetic condition in a particular person and/or their family. (healthline.com)
Research genetic testing, on the other hand, occurs when genetic testing is done on a person who volunteers for a clinical trial. (healthline.com)
We retrospectively analyzed the genetic and clinical characteristics of 111 non-21OHD PAI patients in China. (bmj.com)
Metagenomic sequencing engables a thorough mapping of the genetic material in the clinical sample, providing a broader understanding of the microbiological profile. (folkhalsomyndigheten.se)
The result of of metagenomic sequencing is the outcome of a collaborative effort between bioinformaticians and clinical microbiologists, where the clinical interpretation confirms and complements the technical analysis. (folkhalsomyndigheten.se)
16S sequencing is performed to determine which bacterial species are present in a clinical sample or isolate. (folkhalsomyndigheten.se)

Differential gene expression analysis indicated that this isolate formed excessive biofilm by reducing flagellar formation (7.4 to 1,624.1 folds) and overproducing extracellular matrix components including CdrA (4.4 folds), alginate (5.2 to 29.1 folds) and Pel (4.8-5.5 folds). (frontiersin.org)
SigFuge: single gene clustering of RNA-seq reveals differential isoform usage among cancer samples. (genomeweb.com)
How to Do Differential Gene Expression Analysis? (pharmiweb.com)
Differential Gene Expression analysis is a fundamental tool for identifying genes whose expression levels significantly differ between experimental conditions or biological samples. (pharmiweb.com)

Genome sequencing of normal cells reveals developmental lineages and mutational processes. (genomeweb.com)
Analysis of binding kinetics reveals that this is primarily due to differences in dissociation rate constants. (elifesciences.org)

DGE analysis provides critical insights into the genetic programs governing various stages of development. (pharmiweb.com)
Although such studies provided insights into the location of mtDNA promoters in the mentioned organisms, the techniques used were typically low throughput, were only semi quantitative, challenging to apply and require prior sequence knowledge. (biorxiv.org)

People may opt to have genetic testing done during pregnancy to rule out specific hereditary conditions, such as Down syndrome or potential problems with the unborn child's number of sex chromosomes. (healthline.com)

Genetic testing can also measure results of gene changes, like an RNA analysis of a gene's expression. (healthline.com)

not been previously tested with the same germline test using NGS for the same germline genetic content. (cms.gov)

After sample dropoff, core staff conducts sample QC, which includes Qubit concentration measurement and Bioanalyzer fragment analysis, prior to mtDNA enrichment and library construction. (northwestern.edu)
It's a shame this new fragment of the RdRp does not overlap with that sequence from the recent South African "nearest match" to MERS-CoV. (blogspot.com)

In the method developed at UCLA, scientists analyzed the ratios of slightly different genetic sequences within the isoforms, enabling them to detect important but subtle differences in the genetic sequences. (uclahealth.org)
In contrast, the conventional analysis aggregates all of the isoforms together, meaning that the technique misses important differences within the isoforms. (uclahealth.org)

Anatomy9

Organisms18

Diseases2

Chemicals and Drugs103

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes93

Disciplines and Occupations3

Information Science7

Health Care5

Single-Cell RNA8

Ribosomal RNA genes3

Genomic6

Genomics5

Proteins10

Mutations10

High-throughput6

Nucleic acid4

Polymerase6

Variants14

Whole genome6

Analyze5

Molecules3

Bioinformatics4

Transcriptome5

Genotypes6

Molecular6

Phylogenetic analyses1

Exome3

Viral4

Epigenetic3

Amplification1

MRNA2

Microbial4

Abstract1

Data21

Assays2

MiSeq1

Detection4

Github.com1

Workflow2

Enables3

Transcriptomic1

Methods3

Viruses10

Investigate1

Additionally1

MtDNA sequence2

Datasets1

Specimens1

Heterogeneity1

Functional1

Allele-specific expression1

Diagnostic6

Variant3

Tissue3

Isolates1

Next-generation8

Pipelines1

Illumina1

Microbiome analysis2

Mitochondrial genome2

Variation3

Simultaneously2

Applied Biosystems1

Methodologies2

Amplify2

Clinical10

Differential4

Reveals2

Insights2

Chromosomes1

Gene's1

Germline genetic1

Fragment2

Differences2