• Thus, the Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR) technique was used to examine the genetic variability among individuals of S . olivacea from Setiu Wetlands, Terengganu. (mabjournal.com)
  • Twenty reproducible RAPD fragments generated by OPA 08 and OPA 10 with 17 fragments were polymorphic. (mabjournal.com)
  • Population genetic structure in the fresh water anomuran Aegla jujuyana by RAPD analysis, Journal of Crustacean Biology, 17: 269-274. (mabjournal.com)
  • Application of Random Amplified Polymorphic DNA (RAPD) in Molecular Ecology. (mabjournal.com)
  • Genetic Diversity and Species Diagnostic Markers of Mud Crabs (Genus Scylla) in Eastern Thailand Determined by RAPD Analysis. (mabjournal.com)
  • PZR ile PZR tabanl olan Rastgele o alt lm Polimorfik DNA (Random Amplified Polymorphic DNA =RAPD) ve Restriction Fragment Length Polymorphism (RFLP) y ntemleriyle et t rlerinin ay r m yap lm t r 11 - 13 . (fusabil.org)
  • Analysis of differentiation (genetic diversity and related relationships) among 22 landrace ( Ficus carica L. sativa ) and 2 wild form ( F. carica L. caprificus ) accessions of fig growing under the same environmental conditions in the Palestinian Fig Collection, Til, Nablus, Palestine, using PCR-based Random Amplified Polymorphic DNA (RAPD) and pomological markers, revealed considerable genetic diversity. (scirp.org)
  • Khadari, B., Lashermes, P.H. and Kjellberg, F. (1995) RAPD Fingerprints for Identification and Genetic Characterization of Fig (Ficus carica L.) Genotypes. (scirp.org)
  • Aim: To measure the agreement of methods for identification of Candida species in oral cavity samples, comparing the CHROMagar Candida, microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
  • Methods: Ninety-one colonies of Candida were isolated and presumptively identified in CHROMagar Candida, submitted to microculture, API 20C AUX and RAPD techniques. (bvsalud.org)
  • The RAPD (Random Amplification Polymorphic DNA) allows the amplification of DNA sequences and is a simple and quick technique that does not require prior knowledge on the genomes to characterize organisms, using one randomly determined (usually a decamer) primer 17 . (bvsalud.org)
  • have been studied by RAPD (random amplified polymorphic DNA) and statistical cluster analysis. (jipb.net)
  • The results indicate that the application of RAPD technique in the taxonomic study has gained some resolution which could not be obtained by morphological analysis and has provided available data for developing longan production and for breeding new longan cultivars. (jipb.net)
  • Population Genetic Structure of a Dominant Desert Tree, Haloxylon ammodendron (Chenopodiaceae), in the Southeast Gurbantunggut Desert Detected by RAPD and ISSR Markers [J]. J Integr Plant Biol. (jipb.net)
  • A total of 78 RAPD primers were used and 8 presented differences in band patterns with 54% of the amplified polymorphic fragments. (scielo.br)
  • A caracterização molecular foi baseada em marcadores RAPD, sequenciamento da região do espaçador interno transcrito do DNA ribossomal e "fingerprinting" telomérico. (scielo.br)
  • Foi usado um total de 78 primers RAPD, sendo que 8 apresentaram 54% de fragmentos de DNA polimórficos. (scielo.br)
  • The aim of the current study was to estimate the genetic diversity of F. graminearum and F. culmorum isolated from wheat in Adana province of Turkey by investigating by RAPD-PCR polymorphisms. (scialert.net)
  • Genetic distances of F. graminearum and F. culmorum isolates appeared more intense in wheat fields in Adana region were determined by RAPD-PCR (10 primers). (scialert.net)
  • Restriction Fragment Length Polymorphism (RFLP), Random Amplified Polymorphic DNA (RAPD), Amplified Fragment Length Polymorphism (AFLP), Single-Strand Conformation Polymorphism (SSCP) and Microsatellite DNA. (biomedcentral.com)
  • Populations specific genetic markers and diseases. (mabjournal.com)
  • Agarwal, M., Shrivastava, N. and Padth, H. (2008) Advances in Molecular Markers Techniques and Their Applications in Plant Sciences. (scirp.org)
  • Almajali, D., Abdel-Ghani, A.H. and Migdadi, H. (2012) Evaluation of Genetic Diversity among Jordanian Fig Germplasm Accessions by Morphological Traits and ISSR Markers. (scirp.org)
  • In the present study, a total of 99 accessions of Brassicaceae family, including 84 mustard (50 oilseed and 34 vegetable types) in China and 15 other Brassicaceae accessions were evaluated for their genetic diversity using nuclear and mitochondrial molecular markers. (mdpi.com)
  • Amplified fragment length polymorphism (AFLP) markers were used to detect the relationships among 12 wild accessions and 62 groundcover chrysanthemum cultivars. (ashs.org)
  • Isolates of C. zeae-maydis can be distinguished by two genetic groups (I and II) based on AFLP markers and on polymorphisms of the ITS and 5.8S rDNA regions. (embrapa.br)
  • Whereas the two genetic markers yielded rather well resolved and congruent phylogenies, a cladistic analysis of the anatomical data resulted in an unresolved polytomy. (malacsoc.org.uk)
  • A number of markers are now available to detect nuclear DNA polymorphisms. (intechopen.com)
  • In genetic diversity studies, the most frequently used markers are microsatellites. (intechopen.com)
  • 4, 5 The process involves use of many evenly spaced polymorphic DNA markers to create landmarks across each chromosome. (bmj.com)
  • Genetic variability of trypanosomatids isolated from phytophagous hemiptera defined by morphological, biochemical, and molecular taxonomic markers. (microbiologyresearch.org)
  • Advances in molecular biotechnology have introduced new generations of molecular markers for use in the genetic improvement of farm animals. (biomedcentral.com)
  • This review gives a brief summary on the development of genetic markers including both the classical genetic markers and more advanced DNA-based molecular markers. (biomedcentral.com)
  • This review will help us better understand the characteristics of different genetic markers and the genetic diversity of animal genetic resources. (biomedcentral.com)
  • However, sufficient genetic markers for evaluating the population structure and other aspects of available animal genetic resources are necessary to assess genetic diversity. (biomedcentral.com)
  • By the 1980s, many different types of DNA molecular markers had been explored, e.g. (biomedcentral.com)
  • All of these DNA-based markers contain specific advantages and have played significant roles in the evaluation of genetic diversity in farm animals. (biomedcentral.com)
  • such information is much more precise for the assessment of genetic diversity than previous markers. (biomedcentral.com)
  • The following is a brief summary on the principles and advancements of primary genetic markers involved in assessments of Animal Genetic Resources (AnGR). (biomedcentral.com)
  • The evaluation of farm animal genetic resources through morphological markers is based on subjective judgments and descriptions, and the conclusions reached are often not completely accurate. (biomedcentral.com)
  • Chromosomes are the carriers of genetic material and chromosome mutations are crucial sources of genetic variation [ 5 ], we can use these mutations as markers to determine the specific location of a gene on the chromosome, and its position relative to other genes. (biomedcentral.com)
  • The family includes proteins which bind to both double- and single-stranded DNA and also includes specific DNA binding proteins in serum which can be used as markers for malignant diseases. (lookformedical.com)
  • The percentage of polymorphic bands was 85% indicates that the population of S. olivacea has high level of polymorphism due to low inbreeding factor within a population. (mabjournal.com)
  • The special cuhivars, "Lidongben", "Nanhu Jiaohe" and "Lizhiben" were named respectively as Lidongben group, Nanhu Jiaohe group, and Lizhiben group, because of their more characteristic DNA polymorphism tested in all respective cuhivars. (jipb.net)
  • alata ) using amplified fragment length polymorphism (AFLP) technique. (open-science-repository.com)
  • 1999. Genetic correlations between life-history and behavioral traits can cause reproductive isolation. (scielo.br)
  • Note to the Reader Please cite this work as: Williams RW, Gu J, Qi S, Lu L (2001) The genetic structure of recombinant inbred mice: High-resolution consensus maps for complex trait analysis. (nervenet.org)
  • Among all accessions, genetic relationship was the most relevant factor in AFLP-marker clustering, whereas petal type was also informative. (ashs.org)
  • AFLP technology could be very efficient for discriminating species of chrysanthemum and its related genera and reconstruct their genetic relatedness. (ashs.org)
  • A total of 170 AFLP fragments were detected and all are polymorphic. (open-science-repository.com)
  • Three primer combinations that produced clear scorable and highly polymorphic bands were screened from 64 Eco RI and Ms eI primer combinations. (open-science-repository.com)
  • Most commonly, genotyping methods take advantage of variation in highly polymorphic genes of Plasmodium falciparum , including merozoite surface proteins 1 and 2( msp1 and msp2 ) and glutamine-rich protein ( glurp ) [ 2 ]. (biomedcentral.com)
  • Mathematical model for studying genetic variation in terms of restriction endonuclease. (mabjournal.com)
  • Assessment of Genetic Variation and Distribution Pattern of Thalictrum petaloideum Detected by RAPDs [J]. J Integr Plant Biol. (jipb.net)
  • Jaccard's coefficient of similarity varied from 0.64 to 0.89, indicating much genetic variation in chrysanthemums. (ashs.org)
  • Genetic variation among faba bean genotypes is imperative for their efficient utilization in plant breeding programs and effective conservation. (ppjonline.org)
  • The present study demonstrates fine-scale spatial patterns of genetic variation and contemporary gene flow of red panda ( Ailurus fulgens ) populations with respect to landscape connectivity in Kangchenjunga Landscape (KL), India. (nature.com)
  • In order to find those anatomical characters that statistically discriminate among higher basommatophoran taxa, we performed discriminant analyses (DA) with DNA lineages as a grouping variable. (malacsoc.org.uk)
  • However, morphological and randomly amplified polymorphic DNA analyses confirmed that H. trimorpha sp. (microbiologyresearch.org)
  • Genetic and molecular analyses have led to the identification, cloning and characterization of a few genes that contribute to parasitic fitness in the pathogens. (sisef.it)
  • In this case, the basic principle is to detect specific DNA and RNA sequences via hybridization of a probe, detecting any complementary sequence on the cDNA of this bacterium. (australiaessays.com)
  • Its principle is based on the use of DNA polymerase which is an in vitro replication of specific DNA sequences. (intechopen.com)
  • We can therefore amplify nucleotide sequences from infinitesimal amounts of DNA extract. (intechopen.com)
  • DNA sequences which are recognized (directly or indirectly) and bound by a DNA-dependent RNA polymerase during the initiation of transcription. (lookformedical.com)
  • Cis-acting DNA sequences which can increase transcription of genes. (lookformedical.com)
  • 2000). The main advantage in this context is that the complex genetic and epigenetic correlations among interconnected parts of the brain can be explored using complementary molecular, developmental, structural, pharmacological, and behavioral techniques. (nervenet.org)
  • Effect of Development Stage on the Artemisinin Content and the Sequence Characterized Amplified Region (SCAR) Marker of High-Artemisinin Yielding Strains of Artemisia annua L. [J]. J Integr Plant Biol. (jipb.net)
  • With the release of a genome wide SNPs database, it has become feasible to analyse these genetically determined QTLs for genes polymorphic in these strains. (bmj.com)
  • Previously, classical genetic studies involving cross breeding of mouse strains with differing susceptibilities have identified chromosomal areas associated with predisposition to developing spontaneous and chemically induced lung adenomas. (bmj.com)
  • To discriminate P. falciparum strains, the sizes of amplified products of these genes are compared, routinely based on migration patterns with agarose-gel electrophoresis because of ease of use and low cost. (biomedcentral.com)
  • The monomorphic fragments of 600 bp (P. expansum) and 594 bp (P. griseoroseum) were amplified. (scielo.br)
  • Aljane, F. and Ferchichi, A. (2009) Assessment of Genetic Diversity among Some Southern Tunisian Fig (Ficus carica L.) Cultivars Based on Morphological Descriptors. (scirp.org)
  • Nevertheless, the genetic variability of this species are still poorly studied and need to be further clarified. (mabjournal.com)
  • Genetic variability in the mud crab genus Scylla (Brachyura: Portunidae). (mabjournal.com)
  • Study on genetic variability of Groupers (Epinephelis spp. (mabjournal.com)
  • Genetic diversity, extent of variability and indigenous traditional knowledge of Mucuna adans. (livedna.net)
  • Both gel and capillary methods evaluated polymorphic regions of the merozoite surface protein 1 and 2 and glutamine rich protein genes. (biomedcentral.com)
  • An enzyme capable of hydrolyzing highly polymerized DNA by splitting phosphodiester linkages, preferentially adjacent to a pyrimidine nucleotide. (lookformedical.com)
  • Indeed, if the sequence of interest is present in the DNA extract, it is possible to selectively replicate it (we speak of amplification) in very large numbers. (intechopen.com)
  • The objective of this study was to evaluate the genetic relationships among superior accessions for two varieties of L. pumila ( L. pumila var. (open-science-repository.com)
  • T he important finding of this study is the identification of genetic relationships among the 119 accessions of both varieties with high total phenolic content which can be used for future breeding activities. (open-science-repository.com)
  • Random amplified polymorphic DNA has proven useful in sorting out the relationships of the C. pepo species, varieties, and cultivars, showing that few, if any, modern cultivars have their origins with C. texana. (wikipedia.org)
  • Cannabis sativa L. is one of the best studied species under the aspect of genetic determinism of the sex, but the problem is not yet entirely deciphered. (researchgate.net)
  • It is used for genetic characterization of a range of organisms, plants, animals or microorganisms, including Candida species, for different purposes 18-21 . (bvsalud.org)
  • These species have elevated genomic complexities and limited genetic resources, hindering the application of marker-assisted selection strategies. (bvsalud.org)
  • The development of every species under its particular natural ecosystem, environmental, and socio-economic conditions has led to each having its own specific genetic characteristics. (biomedcentral.com)
  • They are used in the identification, classification, and characterization of genetic evolution of different species or populations. (biomedcentral.com)
  • The genomic DNA was extracted from ten samples of mud crab by using DNeasy kit. (mabjournal.com)
  • Currently, the most promising approach for increasing genetic gains in plant breeding is genomic selection. (bvsalud.org)
  • In this context, the identification of such contaminants is important to avoid compromising further selection cycles, as well as genetic and genomic studies. (bvsalud.org)
  • One could say the primary limitation to crop improvement is no longer, "can we make a precise sequence change in a precise location in eukaryotic genomic DNA? (frontiersin.org)
  • This has been done using techniques such as polymerase chain reaction, gene restriction, use of probe hybridization and also using primers. (australiaessays.com)
  • Some researchers focus on the negative consequences of domestication, whereas others assert that feral populations may serve as useful pools of genetic diversity for future crop improvement. (weizmann.ac.il)
  • Indeed, the genetic distance between some S. japonicum populations is so immense as to indicate that they represent distinct taxa [ 10 ]. (biomedcentral.com)
  • Genomics and changes associated with gene patterns have enabled the prediction of etiological factors leading to a disease being shed light on genetic mapping and prognosis on alterations of DNA in novel diseases. (australiaessays.com)
  • Based on a combination of mitochondrial and nuclear DNA, the following questions were addressed: 1) the phylogeography of the two O. h. robertsoni phylogroups, 2) regional and local population structure in space and time, and 3) patterns of local dispersal under different isolation-by-distance scenarios. (biomedcentral.com)
  • Journal of Genetic Engineering and Biotechnology, 10, 169-175. (scirp.org)
  • One understudied opportunity is using feral crops-plants that have escaped and persisted without cultivation-as a source of genetic diversity, which could build resilience in domesticated conspecifics. (weizmann.ac.il)
  • Classification of Trypanosomatids from insects and plants by the UP-PCR (universally primed PCR) technique and cross dot blot hybridization of PCR products. (microbiologyresearch.org)
  • These diagnostic methods are at the cellular and molecular level since both DNA and proteins are major components of a cell. (australiaessays.com)
  • A method for determining the sequence specificity of DNA-binding proteins. (lookformedical.com)
  • Proteins which bind to DNA. (lookformedical.com)
  • Endogenous substances, usually proteins, which are effective in the initiation, stimulation, or termination of the genetic transcription process. (lookformedical.com)
  • Stress Treatments and DNA Methylation Affected the Somatic Embryogenesis of Citrus Callus [J]. J Integr Plant Biol. (jipb.net)
  • These eight edible cultivated varieties of C. pepo vary widely in shape and color, and one inedible cultivated variety: Due to their varied genetic background, members of C. pepo vary widely in appearance, primarily in regards to their fruits. (wikipedia.org)
  • Plant transformation techniques are well developed for making transgenic varieties in certain crops and model organisms, yet reagent delivery and plant regeneration remain key bottlenecks to applying the technology of gene editing to most crops. (frontiersin.org)
  • Nineteen EcoR I/ Mse I primer combinations revealed 452 informative polymorphic bands with a mean of 23.8 bands and 71.5% polymorphic rate per primer pair. (ashs.org)
  • At present, DNA molecular marker techniques are widely applied in the fields of germplasm identification, phylogenetics, and genetic structural analysis. (biomedcentral.com)
  • These questions, which emerged from the colloquium "Darwins' reversals: What we now know about Feralization and Crop Wild Relatives" at the BOTANY 2021 conference, fall into seven categories that span both basic and applied research: (1) definitions and drivers of ferality, (2) genetic architecture and pathway, (3) evolutionary history and biogeography, (4) agronomy and breeding, (5) fundamental and applied ecology, (6) collecting and conservation, and (7) taxonomy and best practices. (weizmann.ac.il)
  • This restricted movement may lead to genetic consequences including disruption of gene flow, inflation of inbreeding and loss of rare alleles supporting local adaptation and genetic fitness 6 , 7 . (nature.com)
  • Eukaryotic and prokaryotic organisms normally contain DNA in a double-stranded state, yet several important biological processes transiently involve single-stranded regions. (lookformedical.com)
  • The experiment aimed to compare the effects of BA and mT on the regeneration from explants of Daphne mezereum , on the levels of several chemical components, the catalase activity, and to assess genetic diversity of microcuttings. (mdpi.com)
  • Genetic correlations between loci on different chromosomes can be high and can generate false positive linkage. (nervenet.org)
  • Conclusions: API 20C AUX presented the best agreement with molecular random identification and CHROMagar showed good agreement for C. albicans, C. tropicalis, C. dubliniensis and C. krusei identification. (bvsalud.org)
  • Ribosomal DNA restriction analysis and synthetic oligonucleotide probing in the identification of genera of lower trypanosomatids. (microbiologyresearch.org)
  • Genome sequence of Sclerotinia sclerotiorum hypovirulence-associated DNA virus-1 found in the fungus Penicillium olsonii isolated from Washington state, USA. (usda.gov)
  • Analysis of the Genetic Structure of Sclerotinia sclerotiorum (Lib. (jipb.net)
  • Mitochondrial DNA diversity of mud crab Scylla olivacea (Portunidae) in Peninsular Malaysia: a preliminary assessment. (mabjournal.com)
  • 109. Qi P, Eudy D, Schnable JC, Schmutz J, Raymer PL, Devos KM (2019) High density genetic maps of seashore paspalum using genotyping-by-sequencing and their relationship to the Sorghum bicolor genome. (uga.edu)
  • In addition, with biotechnological and computer innovations, novel strategies such as whole-genome SNP chips and DNA Barcoding have emerged. (biomedcentral.com)
  • In many crops, genome engineering techniques have enabled researchers and breeders to take advantage of a vast knowledge base of plant physiology, pathology, and genetics. (frontiersin.org)
  • In addition to the approximately 2300 tests for individual genetic disorders, recent advances in technology have enabled the development of clinical tests which quickly and economically analyze the entire human genome. (cdc.gov)
  • There is evidence that, although incidence is almost always associated with environmental factors such as smoking or occupational exposure, susceptibility has a genetic component with early onset lung cancer following Mendelian inheritance. (bmj.com)
  • After nearly 100 years of documented Dutch elm disease (DED) presence, it seems only fitting to revisit this pathosystem from a genetic standpoint encompassing both traditional Mendelian genetics and the most recent developments in omic-based approaches. (sisef.it)
  • Mendelian genetic analysis of the pathogens is therefore easy and the heritability of any scorable phenotypic or genotypic trait can be assessed rapidly. (sisef.it)
  • In recent decades, traditional methods of microorganism phenotyping have been replaced or added by the procedures associated to recombinant DNA 12-14 . (bvsalud.org)
  • They offer compelling advantages for mapping complex genetic traits, particularly those that have low heritabilities. (nervenet.org)
  • A deoxyribonucleotide polymer that is the primary genetic material of all cells. (lookformedical.com)
  • Sex differences at the genetic/epigenetic level in early development. (ubc.ca)
  • Genetic diversity analysis is a fundamental work for effective management and utilization of plant germplasm. (mdpi.com)
  • Molecular and omic approaches, however, represent technological advances that should allow rapid developments in the genetic analysis of elms and bark beetle vectors of the disease. (sisef.it)
  • Molecular technology has been widely used in analyzing genetic relationships and diversity. (ashs.org)
  • Gene editing tools are being applied in basic research to close the knowledge gap, but the requirements for gene editing techniques for agriculture are different than those for research. (frontiersin.org)
  • A Feature Paper should be a substantial original Article that involves several techniques or approaches, provides an outlook for future research directions and describes possible research applications. (mdpi.com)
  • The proposed pipeline was made available through the polyCID Shiny app and can be easily coupled with traditional genetic approaches, such as linkage map construction, thereby increasing the efficiency of breeding programs. (bvsalud.org)
  • During the last decades, the development of ever more powerful genetic, molecular and omic approaches has provided plant pathologists with a wide array of experimental tools for elucidating the intricacies of plant-pathogen interactions and proposing new control strategies. (sisef.it)
  • This study investigated whether isolates from the two genetic groups differ in. (embrapa.br)
  • Seed lot culture maintenance techniques (seed-lot systems) are used so that the viable micro-organisms used for inoculation are not more than 5 passages removed from the original master seed-lot. (top5binarybrokers.com)
  • 80] present a pilot clinical study evaluating the feasibility of endoscopic transluminal omental patch closure for perforated peptic selleck chemicals llc ulcers, with the hypothesis that the technique will be successful at closing ulcer perforations, as evidenced by intraoperative leak test and post operative water-soluble contrast studies. (hsp-signal.com)
  • The considerable medical importance of S. japonicum has spurred numerous parasitological, ecological and genetic studies. (biomedcentral.com)
  • Taxonomic Study of Dimocarpus longan by Random Amplified Polymorphic DNA Technique[J]. J Integr Plant Biol. (jipb.net)
  • Exploiting Nitrogen-Fixing Rhizobial Symbionts Genetic Resources for Improving Phytoremediation of Contaminated Soils. (unifi.it)
  • Consequently, more accurate genetic information can be obtained to better understand existing animal genetic resources. (biomedcentral.com)
  • Mankind can learn and make use of these special genetic resources to develop animal production for human food needs. (biomedcentral.com)