• sequence
  • DNA sequence analysis has been particularly helpful for identifying point mutations in genes associated with extended-spectrum-β-lactamases (ESBLs) and resistance to carbapenems, fluoroquinolones, oxazolidinones, and antimycobacterial drugs. (asmscience.org)
  • Currently, the rapid accumulation of bacterial whole-genome sequence data for Roseobacter prompted us to investigate Roseobacter evolution from a genomic perspective. (pubmedcentralcanada.ca)
  • Various metabolic processes were examined on a 1.5% Bacto-Agar with various organic compounds, incubated at 50-70 degrees C. 16S rRNA gene sequencing places the family of H. thermophilus, Aquificaceae, in close phylogenetic relationship to the family Aquifex based on 88.5% to 88.9% sequence similarity. (wikipedia.org)
  • citation needed] The nucleic acid sequence of a protein coding gene may also be conserved by other selective pressures. (wikipedia.org)
  • organisms
  • La protein is found in the nucleus in all eukaryotic organisms and associates with several types of RNAs transcribed by RNA pol III. (wikipedia.org)
  • When prokaryotic organisms transcribe DNA into RNA it involves in many cases the binding of a sigma factor upstream of the transcription start site. (dtu.dk)
  • 1 . A method for interspecies differentiation of pathogenic organisms, comprising analysis of the P3 and/or P19 hypervariable region(s) of the RNase P RNA gene. (google.fr)
  • hence at least in these organisms, RNase P is a ribozyme (an RNA molecule catalysing chemical reactions). (google.fr)
  • Horizontal gene transfer (HGT) or lateral gene transfer (LGT) is the movement of genetic material between unicellular and/or multicellular organisms other than by the ("vertical") transmission of DNA from parent to offspring. (wikipedia.org)
  • Most thinking in genetics has focused upon vertical transfer, but horizontal gene transfer is important, and among single-celled organisms is perhaps the dominant form of genetic transfer. (wikipedia.org)
  • Studies in the 1960's used DNA hybridization and protein cross-reactivity techniques to measure similarity between known orthologous proteins, such as hemoglobin and Cytochrome C. In 1965, Émile Zuckerkandl and Linus Pauling introduced the concept of the molecular clock, proposing that steady rates of mutation could be used to estimate the time since two organisms diverged. (wikipedia.org)
  • genomes
  • Approximately 60% of the sequenced reductive dehalogenase genes were broadly distributed, being identified in four or more samples, and often in previously sequenced genomes as well. (frontiersin.org)
  • Primers
  • Therein, we used the common universal primers Bac8f and UN1541r to obtain a nearly full-length gene. (frontiersin.org)
  • The primers developed in this study are thus advantageous for functional characterization of foreign 16S rRNA in E. coli with no artifacts. (frontiersin.org)
  • A suite of degenerate PCR primers targeting a comprehensive curated set of reductive dehalogenase genes was designed and applied to 12 DNA samples extracted from contaminated and pristine sites, as well as six enrichment cultures capable of reducing chlorinated compounds to non-toxic end-products. (frontiersin.org)
  • chromosome
  • The prepilin peptidase (called PibD in crenarchaeota and FlaK in euryarchaeota) is essential for the maturation of the archaellins and is generally encoded elsewhere on the chromosome. (wikipedia.org)
  • A transposable element (TE) (also called a transposon or jumping gene) is a mobile segment of DNA that can sometimes pick up a resistance gene and insert it into a plasmid or chromosome, thereby inducing horizontal gene transfer of antibiotic resistance. (wikipedia.org)
  • It was found to consist of 1,743,135 base pairs arranged in a circular chromosome with an estimated 1,864 protein coding genes and 22 pseudogenes. (wikipedia.org)
  • The genome of Methylobacillus flagellatus is a circular chromosome that is approximately 3Mbp long, and it encodes about 2,766 proteins. (wikipedia.org)
  • soil
  • where resistance genes are obtained either from the indigenous human microbiota or from environmental microorganisms to which an individual is exposed (e.g., via food, water or soil). (frontiersin.org)
  • prokaryotic
  • In this exercise we will look at σ 70 (or RpoD) which is the 'housekeeping' sigma factor responsible for regulating most genes in the prokaryotic cell. (dtu.dk)
  • horizontal gene tra
  • A core set of 694 genes with vertical descent signal that are resistant to horizontal gene transfer (HGT) is used to reconstruct a robust organismal phylogeny. (pubmedcentralcanada.ca)
  • Artificial horizontal gene transfer is a form of genetic engineering. (wikipedia.org)
  • Some Lepidoptera (e.g. monarch butterflies and silkworms) have been genetically modified by horizontal gene transfer from the wasp bracovirus. (wikipedia.org)
  • There are several mechanisms for horizontal gene transfer: Transformation, the genetic alteration of a cell resulting from the introduction, uptake and expression of foreign genetic material (DNA or RNA). (wikipedia.org)
  • Whereas
  • 90% amino acid identity with known gene products, whereas on 13 inserts no resistance gene could be identified with high confidence, in which case we predicted resistance to be mainly mediated by antibiotic efflux. (frontiersin.org)
  • strains
  • Using Web Services, we shall construct a workflow to investigate how this promoter structure is conserved throughout different bacterial strains. (dtu.dk)
  • Genes encoding surface-associated proteins and restriction-modification systems were especially diverse within and between strains. (stanford.edu)
  • synthesis
  • In Escherichia coli, 5S rRNA gene deletions reduce the protein synthesis rate and have a more profound detrimental effect on cell fitness than deletions of a comparable number of copies of other (16S and 23S) rRNA genes. (wikipedia.org)
  • Of particular interest is the HGT of eleven genes involved in vitamin B12 synthesis as well as key enzynmes for dimethylsulfoniopropionate (DMSP) degradation. (pubmedcentralcanada.ca)
  • obligate
  • In addition, it is an obligate chemolithoautotroph, and so genes commonly used in carbon fixation were present. (wikipedia.org)
  • Phytoplasmas are obligate bacterial parasites of plant phloem tissue and of the insect vectors that are involved in their plant-to-plant transmission. (wikipedia.org)
  • reductive
  • The amplified gene products from four environmental sites and two enrichment cultures were sequenced using Illumina HiSeq, and the reductive dehalogenase complement of each sample determined. (frontiersin.org)
  • Genes that encode proteins involved in the RTCA (reductive tricyclic acid cycle) and gluconeogenesis were observed. (wikipedia.org)
  • organism's
  • Consensus exists among evolutionary biologists that one should not separate an organism's genes from the context of its resident microbes. (wikipedia.org)
  • archaeal
  • This led microbiologists to believe that the archaeal motility structure is not only functionally, but also structurally reminiscent of bacterial flagella. (wikipedia.org)
  • bacteriophage
  • 4. The improved expression vector of claim 1 wherein said vector further comprises a mutant cI repressor gene derived from λ bacteriophage which mutant gene codes for a temperature sensitive repressor protein. (freepatentsonline.com)
  • Transduction, the process in which bacterial DNA is moved from one bacterium to another by a virus (a bacteriophage, or phage). (wikipedia.org)
  • genetic
  • Most proteins that make up the archaellum are encoded within one genetic locus. (wikipedia.org)
  • This genetic locus contains 7-13 genes which encode proteins involved in either assembly or function of the archaellum. (wikipedia.org)
  • The genetic locus contains genes encoding archaellins (flaA and flaB) - the structural components of the filament - and core components (flaI, flaJ, flaH). (wikipedia.org)
  • The objective of this study was to estimate the genetic parameters for milk β-hydroxybutyrate (BHBA), acetone (Ac), fat protein ratio (FPR) and energy balance (EB) using milk test day records and investigate the effect of early lactation FPR and EB on milk ketone body concentrations. (readbyqxmd.com)
  • There are four major reasons to use genetic tests to identify antimicrobial resistance genes or mutations associated with resistance in bacterial isolates. (asmscience.org)
  • genome
  • The genome also contains a CRISPR region, which is functionally linked to lateral gene transfer, host cell defense, replication, and regulation. (wikipedia.org)
  • lateral gene tra
  • In the mid-1980s, Syvanen predicted that lateral gene transfer existed, had biological significance, and was involved in shaping evolutionary history from the beginning of life on Earth. (wikipedia.org)
  • novel
  • Our prior work uncovered 16S rRNA genes belonging to a novel, as-yet-uncultivated mycoplasma (lineage 'Mnola') in the oral cavity of a premature neonate. (stanford.edu)
  • evolutionary
  • 2005) observe, "additional evidence suggests that gene transfer might also be an important evolutionary mechanism in protist evolution. (wikipedia.org)
  • transcription
  • The kinase phosphorylates a transcription factor, which regulated gene transcription. (wikipedia.org)
  • Usually AHLs do not need additional processing, and bind directly to transcription factors to regulate gene expression. (wikipedia.org)
  • Namely
  • The method relies upon analysis of specific variable regions of the RNase P RNA gene, namely the P3 and/or P19 region(s). (google.fr)
  • Despite
  • Archaellum versus Bacterial flagellum Despite the limited amount of details presently available regarding the structure and assembly of archaellum, it has become increasingly evident from multiple studies that archaella play important roles in a variety of cellular processes in archaea. (wikipedia.org)
  • evolve
  • While initial phylogenies closely matched the fossil record, observations that some genes appeared to evolve at different rates led to the development of theories of molecular evolution. (wikipedia.org)
  • structural
  • In spite of the structural dissimilarities with the bacterial flagellum, the main function thus far attributed for archaellum is swimming in liquid and semi-solid surfaces. (wikipedia.org)
  • 22 crystal structures of the kinase of the Caspase-1 protein were docked using IGEMDock to FDA, Alkaloids, Lactams, Lactones, Flavinoids, Sulfanilamide, Cyclic Imides, and NSAIDs drugs to determine structural correlation for the most effective binders. (wordpress.com)
  • Bacterial RNase P RNAs have been separated into two main structural classes. (google.fr)
  • different
  • Sigma factors also have important regulatory functions and many different factors acts to ensure differential regulation of different classes of genes under different conditions. (dtu.dk)
  • In other words, the rRNA genes are needed constantly by the cell, however required at different amounts depending on the growth rate of the cell. (dtu.dk)
  • found
  • Inhibition of one major protein found in the inflammasome, Caspase-1, has been found to block the activation of IL-1beta thereby blocking the acute inflammation initiated by appliance tightening. (wordpress.com)
  • Gene transfer agents, virus-like elements encoded by the host that are found in the alphaproteobacteria order Rhodobacterales. (wikipedia.org)
  • Molecular signatures in the form of conserved indels (CSIs) and proteins (CSPs) have been found to be uniquely shared by each separate order, providing a means of distinguishing each clade from the other and supporting the view of shared ancestry of the families within each order. (wikipedia.org)
  • involves
  • Bacterial conjugation, a process that involves the transfer of DNA via a plasmid from a donor cell to a recombinant recipient cell during cell-to-cell contact. (wikipedia.org)