• The key TF were identified by functional enrichment analysis from the list of differentially expressed genes identified in liver samples between 35 pigs fed with 1.5% or 3.0% soybean oil. (nature.com)
  • RNA-seq offers several advantages over microarrays or RT-qPCR for detecting differentially expressed genes (DEGs). (biomedcentral.com)
  • These data suggest that following nutrient restriction, the structural integrity of the rumen wall is compromised and there is upregulation of genes involved in the production of ketone bodies and breakdown of pyruvate for cellular energy. (biomedcentral.com)
  • Strikingly, high bone marrow metabolism was associated with an upregulation of genes related to dendritic cells, regulatory T cell activity, and memory T cells phenotypes ( 4 ). (snmjournals.org)
  • The maximum-likelihood dendrogram was created by using the single-nucleotide variant (SNV) phylogenomics (SNVPhyl) pipeline ( https://snvphyl.readthedocs.io/en/latest ) based on SNVs in the core genome. (cdc.gov)
  • The reference genome is Salmonella Heidelberg strain 12-4374 (GenBank accession no. (cdc.gov)
  • The tree is based on a core genome that represents 94% of the reference genome. (cdc.gov)
  • To create and use a custom reference package, Space Ranger requires a reference genome sequence (FASTA file) and gene annotations (GTF file). (10xgenomics.com)
  • Path(s) to FASTA file containing your genome reference. (10xgenomics.com)
  • Path(s) to genes GTF file(s) containing annotated genes for your genome reference. (10xgenomics.com)
  • done Writing genome FASTA file into reference folder. (10xgenomics.com)
  • done Writing genes index file into reference folder (may take over 10 minutes for a 3Gb genome). (10xgenomics.com)
  • done Writing genome metadata JSON file into reference folder. (10xgenomics.com)
  • We have presented a first catalogue of mutated lncRNA genes driving cancer, which will grow and improve with the application of ExInAtor to future tumour genome projects. (nature.com)
  • Whole genome sequencing makes it possible to comprehensively discover the mutations, and the mutated genes, that are responsible for tumour formation. (nature.com)
  • To isolate possible mechanisms underlying these structural and functional changes, we used whole-genome RNA sequencing and found prominent dopamine-induced enrichment of genes containing the CCCTC-binding factor (CTCF) motif, suggesting involvement of chromatin restructuring in the nucleus. (jneurosci.org)
  • In this usage, the main genome browser display presents a graph of summary values (e.g. medians) for each variable, and the distribution of sample values across variables is shown via a boxplot graph shown on the details page for each region. (ucsc.edu)
  • In this study, we explored both hypotheses elucidating the pan-genome of Vibrionaceae isolates after a perturbation event in the endangered oasis of Cuatro Ciénegas Basin (CCB), Mexico, and looking for signals of adaptation to the environments in their genomes.Results We obtained 42 genomes of Vibrionaceae distributed in six lineages, two of them did not showed any close reference strain in databases. (researchsquare.com)
  • Bacterial genomes, in contrast to eukaryotic genomes, usually maintain constant genome sizes [9, 10], suggesting that while horizontal gene transfer (HGT) increases the genome size by adding new genes, selection maintains the genome size by removing deleterious, non-functional or non-useful genes [11-13]. (researchsquare.com)
  • Recombination decreases linkage disequilibrium among genes, allowing selection and the related Hill-Robertson effect to operate in specific genes and avoiding the purged of genetic diversity along with the genome [22, 23]. (researchsquare.com)
  • To be considered for transcriptome alignment, genes must have annotations with feature type 'exon' (column 3) in the GTF file. (10xgenomics.com)
  • Whole transcriptome sequencing (RNA-seq) represents a powerful approach for whole transcriptome gene expression analysis. (biomedcentral.com)
  • The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (AmpliSeq) was recently introduced by Life Technologies as a whole-transcriptome, targeted gene quantification kit to overcome these limitations of RNA-seq. (biomedcentral.com)
  • As one of the most widely used tools for transcriptome profiling, quantification of differential gene expression has played a pivotal role in the identification of pathway and gene-network interactions [ 1 - 3 ]. (biomedcentral.com)
  • For arrays, the SG is built according to the reference transcriptome selected by the user and for RNA-Seq, it is created by predicting the features in the .bam files provided by the user. (bioconductor.org)
  • To build the CDF file (to work under the aroma.affymetrix framework), EventPointer requires a GTF file with the reference transcriptome information. (bioconductor.org)
  • GTF files downloaded from sites like ENSEMBL and UCSC often contain transcripts and genes which need to be filtered from your final annotation. (10xgenomics.com)
  • WARNING: The following transcripts appear on multiple chromosomes in the GTF: This can indicate a problem with the reference or annotations. (10xgenomics.com)
  • Real-time qPCR was used to quantify mRNA transcripts of 26 genes associated with ruminal epithelial function. (biomedcentral.com)
  • The name2 field provides an alternate item name, useful if you would like to associate multiple transcripts to a single gene locus, different variables to the same experiment type, etc. (ucsc.edu)
  • For the quantification of gene transcripts real-time quantitative polymerase chain reaction (RT-qPCR) is the standard technique. (1library.org)
  • These results indicate that astrocyte response to elevated dopamine involves PARP1-mediated CTCF genomic restructuring and concerted expression of gene networks. (jneurosci.org)
  • The software returns a data.frame with the detected alternative splicing events: gene name, type of event (cassette, alternative 3',…,etc), genomic position, statistical significance and increment of the percent spliced in ( \(\Delta \Psi\) ) for all the events. (bioconductor.org)
  • The barChart (and bigBarChart) track format displays a graph of category-specific values over genomic regions, similar to the GTEx Gene track. (ucsc.edu)
  • This format is useful for displaying gene expression and other datasets where it is desirable to compare a set of variables over genomic regions. (ucsc.edu)
  • In our previous article - Facilitating Exploratory Data Visualization: Application to TCGA Genomic Data - we described how to visualize gene expression data using box plots, violin plots, dot plots and stripcharts. (britishcars.pl)
  • In addition, the minimum number of reference genes required for normalization was determined. (1library.org)
  • Based on the three algorithms, we provide a list of genes for cyanobacterial RT-qPCR data normalization. (1library.org)
  • Pairwise variation (V) is definitely an index for determining the optimal variety of reference genes for correct RT-qPCR normalization. (atminhibitor.com)
  • To ascertain the optimal number of reference genes required for samples of body components or nutrient forms, the pairwise variation (Vn/Vn+1) between two sequential normalization elements (NFn and NFn+1) was calculated by geNorm. (atminhibitor.com)
  • A large variation implies that an more reference gene must be abandoned for the calculation of a reputable normalization factor. (atminhibitor.com)
  • Though accepted as the gold-standard for measuring gene expression, this method is lower throughput and not amenable to perform global expression analyses. (biomedcentral.com)
  • Recently, we elucidated the developmental role of tobacco ( Nicotiana tabacum L.) NtTTG2 in association with 12 genes that putatively encode AUXIN RESPONSIVE FACTOR (ARF) proteins, including NtARF8, NtARF17, and NtARF19. (biomedcentral.com)
  • Most of these GC-balanced genes encode Cryptosporidium-specific proteins that have intrinsically disordered regions and are involved in host-parasite interactions. (cdc.gov)
  • Male worms in which the gene for this protein has been 'knocked out' have a normal lifespan, but show signs of aging earlier than normal males. (elifesciences.org)
  • The J protein interacts with the maltose outer membrane porin (the product of the lamB gene) of E. coli, a porin molecule, which is part of the maltose operon. (wikipedia.org)
  • At first, these express the N and cro genes, producing N, Cro and a short inactive protein. (wikipedia.org)
  • We further show that this plasticity depends on activation of genes sensitive to DNA-binding protein CTCF. (jneurosci.org)
  • Some lncRNAs (referred to as long intergenic non-coding RNAs, lincRNAs) are transcribed from separate loci, whereas others are transcribed divergently from promoters of protein-coding genes or in antisense direction to these. (biomedcentral.com)
  • In this work, we have studied the feasibility of providing tissue-specific selectivity to replication-competent adenoviruses through the regulation of the late structural protein fiber (L5 gene). (oncotarget.com)
  • Transcriptional regulation information for a gene, including any predicted DNA binding site motifs ( YeTFaSCo ) for the gene's protein product, as well as any of its targets (genes it regulates) or regulators (genes that regulate it), based on experimental evidence. (yeastgenome.org)
  • Collection of domains associated with the protein based on various sources, including the protein coordinates for the domain, a domain Description, a Source and corresponding accession ID, and the number of S. cerevisiae genes that share the same domain. (yeastgenome.org)
  • The best validated susceptibility variants for Parkinson's disease are located in the alpha-synuclein (SNCA) and microtubule-associated protein tau (MAPT) genes. (uni.lu)
  • METHODS AND RESULTS: We performed the largest multi-center study to ascertain the frequency and pathogenicity of the reported vacuolar protein sorting 35 gene variants in more than 15,000 individuals worldwide. (uni.lu)
  • Actin has been extensively used because the `universal' reference gene (even without any validation) as it is regarded as to be a essential component with the protein scaffold in cytoskeleton maintenance, shape determination, and cell motility. (atminhibitor.com)
  • Different mutations of EDA gene and EDAR gene could make different influence on the protein function and lead to the occurrence of HED. (bvsalud.org)
  • In agreement with this, the fox-derived isolate possesses more subtelomeric genes encoding invasion-related protein families. (cdc.gov)
  • Essential FA, mainly polyunsaturated fatty acids (PUFA), may modulate gene expression in diverse biological processes thought regulating transcription factors (TF), including peroxisome proliferator receptors (PPAR) , liver X receptors (LXR) , and sterol regulatory element-binding proteins ( SREBP ) 3 . (nature.com)
  • 109 Downregulation of SOCS gene expression by siRNA or through the expression of dominant adverse SOCS proteins to enhance cytokine signaling may be helpful for enhancing anti tumor immunity. (micrornaarray.com)
  • HLA-G differs from the other MHC class I genes by its low polymorphism and alternative splicing that generates seven HLA-G proteins, whose tissue-distribution is restricted to normal fetal and adult tissues that display a tolerogeneic function toward both innate and acquired immune cells. (biovendor.com)
  • a map is constructed that shows interactions among molecular entities (such as genes, proteins and RNAs), using information from literature and databases. (hindawi.com)
  • Thus, AmpliSeq stands as a very sensitive and cost-effective approach for very large scale gene expression analysis and mRNA marker screening with high accuracy. (biomedcentral.com)
  • They act by binding to the 3'-untranslated region (3'UTR) of targeted messenger RNAs (mRNAs), promoting either mRNA cleavage or repression of gene expression at post-transcriptional level [ 2 ]. (oncotarget.com)
  • 1990). Characterization and Expression of the Gene for the Human Fc Receptor γ Subunit. (igem.org)
  • Studies sug- terized by flow cytometry using anti between the immune response and the gest that HCV inhibits receptor genes in CD3, CD56 and CD16 monoclonal virus replication rate [5] and play a cru- the activation of NK cells [20], and the antibodies. (who.int)
  • Our study indicates that AmpliSeq excels in the limiting areas of RNA-seq for gene expression quantification analysis. (biomedcentral.com)
  • Our pipeline, ExInAtor, identifies genes with excess load of somatic single nucleotide variants (SNVs) across panels of tumour genomes. (nature.com)
  • BACKGROUND: Variants within the leucine-rich repeat kinase 2 gene are recognized as the most frequent genetic cause of Parkinson's disease. (uni.lu)
  • METHODS: The Genetic Epidemiology of Parkinson's Disease Consortium recently performed the largest genetic association study for variants in the leucine-rich repeat kinase 2 gene across 23 different sites in 15 countries. (uni.lu)
  • A multi-centre clinico-genetic analysis of the VPS35 gene in Parkinson disease indicates reduced penetrance for disease-associated variants. (uni.lu)
  • If **Yes**, the number of variants is also displayed. (lu.se)
  • MicroRNAs (miRNAs) are small noncoding RNA molecules with important regulatory roles in gene expression. (oncotarget.com)
  • MiRNAs appear to target about 60% of the genes of humans and other mammals. (micrornaarray.com)
  • MicroRNAs (miRNAs) are potent effectors in gene regulatory networks where aberrant miRNA expression can contribute to human diseases such as cancer. (hindawi.com)
  • For a better understanding of the regulatory role of miRNAs in coordinating gene expression, we here present a systems biology approach combining data-driven modeling and model-driven experiments. (hindawi.com)
  • Although microRNAs (miRNAs) are physically small, they have been shown to play an important role in gene regulation [ 1 ]. (hindawi.com)
  • The systems biology approach, combining data-driven modeling and model-driven experiments, provides a systematic and comprehensive perspective on the regulatory roles of miRNAs in gene regulatory networks [ 3 - 5 ]. (hindawi.com)
  • The application of the systems biology approach to the analysis of a gene regulatory network is demonstrated with a case study of the regulation of p21 by multiple miRNAs [ 4 ]. (hindawi.com)
  • Screening of suitable reference genes for gene expression using quantitative real-time PCR in Gynura bicolor DC. (scienceasia.org)
  • To date, a systematic exploration of suitable reference genes in different tissues at different developmental stages in G. bicolor has not been performed. (scienceasia.org)
  • ACTIN and GAPDH were selected as the most suitable reference genes in stems. (scienceasia.org)
  • ACTIN was selected as the most suitable gene in all samples, as it was expressed stably in all samples. (scienceasia.org)
  • Overall, this research provides a guideline for the selection of suitable reference genes in qRT-PCR gene expression studies of different tissues at different development stages in G. bicolor. (scienceasia.org)
  • Selection of suitable reference genes for RT-qPCR analyses in cyanobacteria. (1library.org)
  • Citation: Pinto F, Pacheco CC, Ferreira D, Moradas-Ferreira P, Tamagnini P (2012) Selection of Suitable Reference Genes for RT-qPCR Analyses in Cyanobacteria. (1library.org)
  • Leucine-rich repeat kinase 2 variation related to disease susceptibility displays many features that reflect the nature of complex, late-onset sporadic disorders like Parkinson's disease. (uni.lu)
  • click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction. (yeastgenome.org)
  • information about the experiment and assay type, constructs, conditions, and strains, as well as a reference, are provided for each Regulator annotation. (yeastgenome.org)
  • Proto-oncogene lncRNAs include HOTAIR, upregulated in multiple cancers, which recruits the repressive PRC2 chromatin regulatory complex to hundreds of genes 11 . (nature.com)
  • Twenty-four potential transcriptional regulatory pathways were identified based on SDE TFs matched with their corresponding SDE immunological genes. (frontiersin.org)
  • For genes with regulatory targets, shared GO Biological Process terms for these targets are listed. (yeastgenome.org)
  • This table lists putative transcriptional regulatory targets for the central gene represented on this page. (yeastgenome.org)
  • To investigate a gene regulatory network, an iterative process of four steps is needed. (hindawi.com)
  • In turn, these hypotheses, which provide reasonable explanations for the biological phenomenon, lead to an enhanced understanding of the gene regulatory network. (hindawi.com)
  • Significantly differentially expressed (SDE) immunological genes and transcription factor (TF) were selected for functional pathways and transcriptional signaling identification. (frontiersin.org)
  • CTCF binding to promoter sites bidirectionally regulates gene transcription and depends on activation of poly-ADP-ribose polymerase 1 (PARP1). (jneurosci.org)
  • The mutations associated with resistance to many of the antituberculosis drugs have been identified, though much work remains to be done to identify the molecular basis of resistance for some of the drugs and to determine the predictive value of finding a particular mutation in a strain of M. tuberculosis (1,2). (cdc.gov)
  • For example, approximately 95% of rifampin-resistant M. tuberculosis strains carry mutations within the rifampin-resistance determining region (RRDR), an 81-bp region encoding codons 507 through 533 of the rpoB gene. (cdc.gov)
  • Kits for detecting mutations associated with rifampin resistance that are commercially available in Europe and elsewhere include line-probe assays (INNO-LiPA® Rif.TB, Innogenetics and GenoType® MTBDR( plus ), Hain LifeScience GmbH) and microarray assays (CombiChip Mycobacteria DR, GENE IN). (cdc.gov)
  • Objective: To detect gene mutation in patients with hypohidrotic ectodermal dysplasia (HED) by using whole exome sequencing, to analyze the pathogenicity of the mutations, and to provide reference for the genetic diagnosis of HED patients. (bvsalud.org)
  • Whole exome sequencing and sanger sequencing were performed to detect gene mutations. (bvsalud.org)
  • Bioinformatics tools predicted that the mutations of EDA gene detected in this study were highly species conserved and disease-causing. (bvsalud.org)
  • Conclusions: Three reported mutations of EDA gene and a previously unreported mutation of EDAR gene were detected in four HED families. (bvsalud.org)
  • Based on the analysis results from the software programs geNorm, NormFinder and BestKeeper, GAPDH was the most stable reference gene in roots and leaves during different developmental stages. (scienceasia.org)
  • The six genes exhibiting less variation in each organism were evaluated in terms of their expression stability using geNorm, NormFinder and BestKeeper. (1library.org)
  • The ability to regulate transgene expression will be crucial for development of gene therapy to the brain. (lu.se)
  • An aspect of gene delivery to the brain that is currently being regulate the endogenous GFAP expression in a lesioned brain. (lu.se)
  • Cells which lack ER expression at diagnosis arise from an ER compart- were harvested at 60 - 80% confluency and used as a reference in all hybrid- ment within the mammary epithelium or represent evolution from an izations. (lu.se)
  • 2003). The therapeutic effects of different genes delivered by ment of the hGFAP promoter used here has been extensively studied and lentiviral vectors have been documented in a number of animal found to be up-regulated after several different stimuli both in vitro and models, both in rodents and primates, and examples include delivery in vivo in transgenic mice (Brenner et al. (lu.se)
  • Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. (yeastgenome.org)
  • This diagram displays up to the top 30 positive interactions (green), negative interactions (orange), and phenotypes (blue) that are shared between the given allele (black) and other alleles (purple). (yeastgenome.org)
  • Analysis of gene sequences can detect genetic patterns which diagnose disease, characterize microorganisms, and infer drug resistant phenotypes. (smartgene.com)
  • While a barChart track can effectively show datasets with single values for each variable (e.g. comparing individual samples), the format provides specific features to display studies comprised of a large set of samples for each variable (e.g. comparing tissues with multiple samples for each tissue). (ucsc.edu)
  • The ATP12A gene codes for a non-gastric H + /K + ATPase, which is expressed in a wide variety of tissues. (karger.com)
  • Here we show that NtTTG2 regulates tobacco growth and development by involving the NtARF8 , NtARF17 , and NtARF19 genes, with the NtARF8 gene playing a predominant contribution. (biomedcentral.com)
  • Sequencing is a particularly valuable approach when the gene or organism in question can display unknown genetic variability. (smartgene.com)
  • Compute distances between samples rather than genes. (rdrr.io)
  • If False, compute distances between genes. (rdrr.io)
  • Run read_EPSCsims_mdb_alone.m next with the simulated parameter values specified to compute the mean EPSC. (yale.edu)
  • Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. (yeastgenome.org)
  • This diagram displays physical (purple lines) and genetic (green lines) interactions between the given gene (yellow circle) and its interactors (gray circles) based on the number of experiments supporting each interaction (adjustable using the slider at the bottom). (yeastgenome.org)
  • Interactions between the interactors for a given gene are also shown. (yeastgenome.org)
  • Recently, a protective p.N551K-R1398H-K1423K haplotype in the leucine-rich repeat kinase 2 (LRRK2) gene was identified, with p.R1398H appearing to be the most likely functional variant. (uni.lu)
  • Reads aligning non-uniquely to multiple genes cause the pipeline to detect fewer molecules. (10xgenomics.com)
  • The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. (biomedcentral.com)
  • We hypothesized that in animals undergoing compensatory growth there would be an up-regulation of the relative expression of genes involved in VFA absorption and metabolism, as well as genes underlying growth and proliferation of the ruminal epithelium. (biomedcentral.com)
  • We have read with great interest the article "Prognostic Value of Bone Marrow Metabolism on Pretreatment 18 F-FDG PET/CT in Patients with Metastatic Melanoma Treated with anti-PD-1 Therapy" from Nakamoto et al. (snmjournals.org)
  • Patients with high BM glucose metabolism displayed an immunosuppressive phenotype with increased MDSCs and decreased CD8+ T cells ( 8 ). (snmjournals.org)
  • Once sequence data are created in the laboratory, they have permanent value. (smartgene.com)
  • Sequence data, stored securely in SmartGene, are kept available for future analysis and easy reinterpretation, both with updated algorithms and constantly refreshed reference databases. (smartgene.com)
  • Quantitative real-time PCR (qRT-PCR) is a preferred approach for monitoring gene expression levels. (scienceasia.org)
  • The comprehensive evaluation of gene expression patterns is an important part of this characterization and, at present, the quantitative real-time polymerase chain reaction (RT-qPCR) is considered the gold standard for measurement of transcript abundance [6]. (1library.org)
  • The values of parameters in this model can be determind from literature, databases or they are directly estimated from quantitative experimental data using optimization methods. (hindawi.com)
  • WID-qEC Test: The WID®-qEC test utilises quantitative real-time PCR on bisulfite modified DNA to assess DNA methylation (MethyLight) in the GYPC and ZSCAN12 genes, using COL2A1 as a control. (who.int)
  • When analyzing four closely related hiPSC-CM lines, we show that both AmpliSeq and RNA-seq capture similar global gene expression patterns consistent with known sources of variations. (biomedcentral.com)
  • A logical argument whether or not to use individual replicate FPKM values as opposed to condition FPKM estimates. (rdrr.io)
  • A logical argument to log10-transform FPKM values prior to plotting. (rdrr.io)
  • merge: logical or character value. (britishcars.pl)
  • logical value. (britishcars.pl)
  • The use of viral vectors to deliver foreign genes to the brain is highly logical states in the human brain (Eng et al. (lu.se)
  • For the hybridization assays, the region of the target gene associated with resistance is PCR amplified, and the labeled PCR products hybridized to oligonucleotide probes immobilized on a nitrocellulose strip or in a microarray. (cdc.gov)
  • NtARF8 also contributed to NtTTG2-regulated seed production as concurrent NtTTG2 and NtARF8 overexpression played a synergistic role in seed production quantity, whereas concurrent silencing of both genes caused more severe seed abortion than single gene silencing. (biomedcentral.com)
  • For that reason, we conclude that two ideal reference genes had been enough for normalizing expression values of target genes below these tested situations. (atminhibitor.com)
  • A bifurcation analysis will also be performed in order to find crucial parameter values that would drastically change the behavior of the system. (igem.org)
  • Based on the network structure, a detailed mechanistic model is established and its parameter values are determined using data. (hindawi.com)
  • Gene sequencing enables the diagnosis and characterization of human and animal diseases and the detection of environmental pathogens as never before. (smartgene.com)
  • Specify multiple genomes by specifying the --genes argument multiple times. (10xgenomics.com)
  • Background In bacteria, pan-genomes are the result of the evolutionary 'tug of war' between selection and horizontal gene transfer (HGT). (researchsquare.com)
  • While the genomes of C. canis have similar gene contents and organisations, they (~41.0%) and C. felis (39.6%) have GC content much higher than other Cryptosporidium spp. (cdc.gov)
  • Our results propose that a broad pattern of astrocyte responses to dopamine specifically relies on CTCF-dependent gene networks. (jneurosci.org)
  • These studies have generated adenoviruses with miRNA target sites placed in the 3'UTR of early gene products. (oncotarget.com)
  • In vivo , liver production of infective viral particles was highly impaired, similarly to that triggered by an adenovirus with miRNA target sites regulating the early E1A gene. (oncotarget.com)
  • download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine. (yeastgenome.org)
  • shared GO terms, the number of target genes that share them, and the P-value are all indicated in the table. (yeastgenome.org)
  • Screening of the most stable reference genes is paramount to the accuracy of data generated by qRT-PCR. (scienceasia.org)
  • 2015) in which both RPS20 and RPS13 appeared to be poorly stable genes under many situations. (atminhibitor.com)
  • Using published data from two standard RNA reference samples, we observed a strong concordance of log2 fold change for all genes when comparing AmpliSeq to Illumina HiSeq (Pearson's r = 0.92) and Ion Torrent Proton (Pearson's r = 0.92). (biomedcentral.com)
  • These values are used to draw a boxplot of all sample data on the details page for the bar chart. (ucsc.edu)
  • The ggplot function is used to create the dot plot where we first specify the name of the data frame with the information to be displayed and then use the aes argument to list the variables to plot on the horizontal and vertical axes. (britishcars.pl)
  • Furthermore, monitoring of sHLA-G in amniotic fluid and plasma of pregnant women may have an important prognostic value to recognize pathological situations. (biovendor.com)
  • ABSTRACT We evaluated the prognostic value of serum endostatin and vascular endothelial growth factor (VEGF) for diagnosis of pre-eclampsia. (who.int)
  • Cro binds to OR3, preventing access to the PRM promoter, preventing expression of the cI gene. (wikipedia.org)
  • Towards this goal we have selected and analyzed twelve candidate reference genes from three morphologically distinct cyanobacteria grown under routinely used laboratory conditions. (1library.org)
  • Using a stratified approach, we identify 15 high-confidence candidates: 9 novel and 6 known cancer-related genes, including MALAT1, NEAT1 and SAMMSON . (nature.com)
  • This vast population of uncharacterized genes likely includes many with novel roles in cancer. (nature.com)
  • orthogonal exPeriMental changes and pathways induced by EGE investigated the impact of aSSeSSMent of ePigenetic environmental factors in cancer causation, both current H. pylori infection and driver geneS and their link to underpinning studies of etiology e , pigenetic memory of past (eradicated) environMental carcinogenS carcinogen evaluation, and prevention. (who.int)
  • All three statistical methods demonstrate AmpliSeq as a highly accurate method for differential gene expression analysis. (biomedcentral.com)
  • Expression analysis of cognitive decline-related genes was performed using RT-qPCR, and working memory was assessed using a T-maze. (biomedcentral.com)
  • Nonetheless, our study found that Actin displayed quite low stability in comparison to other references particularly within the samples of diverse body parts (Fig. 2A). (atminhibitor.com)
  • Comma separated list of category values. (ucsc.edu)
  • If your manuscript contains footnotes, move the information into the main text or the reference list, depending on the content. (plos.org)
  • EventPointer relies on SGSeq package to obtain the corresponding SGs for every gene present in the experiment. (bioconductor.org)
  • The dataset comprises 394 SNVs, and SH-like branch support values are displayed. (cdc.gov)
  • SARS-CoV-2 detection was conducted by real-time RT-PCR and by digital droplet RT-PCR (dd RT-PCR) using RdRp, gene E and gene N as targets. (bvsalud.org)
  • We analyzed standard reference RNA samples and RNA samples obtained from human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs). (biomedcentral.com)
  • All 5 reference genes displayed a wide range of Cq values in all samples, indicating that they were expressed variably. (scienceasia.org)
  • Creates a ggplot plot object with a geom_tile layer of JS Distance values between samples or genes. (rdrr.io)
  • A ggplot2 plot object with a geom_tile layer to display distance between samples or genes. (rdrr.io)
  • We first evaluated coverage bias by mapping reads from PCR-amplified metagenomes onto reference contigs obtained from unamplified metagenomes of the same samples. (peerj.com)
  • A cut-off value of 0.15 for pairwise variation was recommended.for samples of unique nutrient kinds. (atminhibitor.com)
  • 2011). Within the current study, RPL32 was ranked at the top in stability for samples of distinctive physique parts but ranked lower (because the eighth from the 10 genes) for samples of diverse nutrient varieties. (atminhibitor.com)
  • Traces of the RdRp gene, gene E and gene N were found in 17/77 samples (22%, average concentration 31.2 g.c./cm2, range 5.6 to 132 g.c./cm2), 8/77 samples (10%, average concentration 15.1 g.c./cm2, range 6 to 36 g.c./cm2), and in 1/77 (1%, concentration 7.2 g.c./cm2). (bvsalud.org)
  • The WID™-qEC test assesses DNA methylation changes in specific gene regions by analyzing cervicovaginal smear samples collected from participants. (who.int)
  • Resource is probability to databases of Helps and displays, such gene, today events, normal people, a vital number tumor-suppressor-miRNA, and the management to post-processing model to a 1949Pre-war function. (lightseed.com)
  • the beige colored item (tissue) has the highest expression in the ACE2 gene and represents more than 10% of total expression. (ucsc.edu)