Genes research mice knockout. Medical search. Frequent questions

The first artificial genetic modification accomplished using biotechnology was transgenesis, the process of transferring genes from one organism to another, first accomplished by Herbert Boyer and Stanley Cohen in 1973. (wikipedia.org)
They are widely used in knockout experiments, especially those investigating genetic questions that relate to human physiology . (wikipedia.org)
To understand regulation of gene expression and to delineate the function of mammalian genes, we routinely create transgenic & gene knockout mice by performing DNA microinjection into mouse eggs, and genetic manipulation of mouse embryonic stem cells. (ccmb.res.in)
One naturally occurring genetic defect in mice results in an animal with no hair that is called a nude. (uwm.edu)
DNA microinjections is a common technique used for gene targeting as it involves manipulation and reintroduction of the genetic material into the same species. (freeonlineresearchpapers.com)
This system, called recombineering, has revolutionized genetic engineering techniques, including the modification of genes on bacterial artificial chromosomes (BACs) and the generation of conditional knockout mice. (federallabs.org)
Other diverse uses of the technology include stem cell research, genetic studies in model organisms, creation of research tools such as transgenic mice and specialized imaging vectors, and high-throughput screening. (federallabs.org)
Sir Martin performed pioneering research into stem cells, and used them to create mice with a specific genetic disorder. (lirneasia.net)
Causal impact of a full-gene deletion will be investigated in all organ systems by comparing phenotypic characteristics of KO-mice with wild-type control mice of the same genetic C57BL/6N background (Munoz-Fuentes et al. (springer.com)
Although metabolic alterations are observed in many monogenic and complex genetic disorders , the impact of most mammalian genes on cellular metabolism remains unknown. (bvsalud.org)
More so, TEs are a rich source of genetic variation, which makes them an intriguing research avenue to investigate humanspecific traits, including their impact on human brain evolution and their relevance in disease. (lu.se)

Humans born with mutations that deactivate the p53 gene have Li-Fraumeni syndrome , a condition that dramatically increases the risk of developing bone cancers, breast cancer and blood cancers at an early age. (wikipedia.org)
Mutations in this gene have been associated with an increased susceptibility to HIV-1 infection and rheumatoid arthritis. (thermofisher.com)
But genes don't change from one tissue to the next, and - with the exception of mutations that accrue gradually over a lifetime in particular cells and can lead to cancer and other conditions - they remain largely unaltered by disease and the aging process. (stanford.edu)
In addition, RMCE has great potential to allow researchers to comparatively analyze mutants, characterize domain-specific functions in proteins of interest, generate point mutations, generate large gene mutations, and generate entire replacements of a wildtype gene with a different gene of interest. (genoway.com)
TET2-knockout mice predominantly develop chronic myelomonocytic leukemia (CMML), but they can also develop other myeloproliferative as well as lymphoproliferative diseases, reflecting the prevalence of TET2 mutations in these varied hematologic malignancies. (aacrjournals.org)
Several mutations have been identified in the MUT gene resulting in MMA. (medscape.com)
Mutations of the MUT gene are estimated to cause 60% of MMA cases. (medscape.com)
Candidate genes for cblA , cblB , designated MMAA and MMAB, and mutations of these genes have been elucidated. (medscape.com)
Although there are 13 exons of the MUT gene, the most deleterious mutations were located in exon 12, then exon 9, exon 11, and exon 2. (medscape.com)
We investigated the effect of loss-of-function mutations in 30 unique gene knockout (KO) lines on plasma metabolites, including genes coding for structural proteins (11 of 30), metabolic pathway enzymes (12 of 30) and protein kinases (7 of 30). (bvsalud.org)
Research on IGSF1 nearly ground to a halt until 2011, when next-generation sequencing identified mutations in the X-linked IGSF1 gene in boys and men with congenital central hypothyroidism. (medscape.com)
Limb-girdle muscular dystrophy type 2A is an autosomal recessive disorder generated by inactivating mutations in the gene coding for the muscle specific protease calpain-3. (lu.se)

The Transgenic and Knockout Core can construct gene-targeting vectors for the generation of knockout, knockin and conditional knockout mice. (mayo.edu)

There are several thousand different strains of knockout mice. (wikipedia.org)
A modern mouse facility houses transgenic mice and other experimental mouse strains needed for creation of transgenic and gene knock-out mice. (ccmb.res.in)
There are over 400 documented inbred strains of mice. (uwm.edu)
Two strains may be bred together to produce a mixture of the genes of each strain. (uwm.edu)
A cell culture model, based on wild-type and specific integrin knockout cell lines lacking the integrin subunits αv, β1 or β3, was used to investigate the susceptibility to WNV, and to evaluate binding and replication efficiencies of four distinct strains (New York 1999, Uganda 1937, Sarafend and Dakar). (nih.gov)
The use of genetically defined inbred mouse strains, humanized mice, and gene knockout mice has enabled the research community to study the process of the way pathogens cause diseases, the role of specific host genes in controlling or promoting disease, and potential targets for prevention or identification of treatment for a variety of infectious agents. (medgadget.com)
Mouse knock-out strains demonstrated that T2D-associated candidates regulate glucose homeostasis and body composition in vivo. (lu.se)

Embryonic stem cells are isolated from a mouse blastocyst (a very young embryo ) and grown in vitro . (wikipedia.org)
Most experiments on stem cell therapies are based on mice, and the researchers wanted to understand whether primate embryonic stem cells respond the same way as those of mice do. (livescience.com)
The mouse genetics laboratory includes well-furnished infrastructure for culture of embryonic stem cells, cryopreservation of embryos, micro-manipulation of embryos, animal surgery, construction of transgenes & gene knock-out vectors, identification of transgenic & knock-out mice, and analysis of gene expression. (ccmb.res.in)
At present, this is only possible with mice, using so-called embryonic stem cells. (newscientist.com)
Deletion of the STAT3 gene in knock-out mice was lethal at the early embryonic stage. (thermofisher.com)
Most common and most controversial is embryonic stem-cell mediated gene transfer. (freeonlineresearchpapers.com)
It involves introduction of modified DNA into embryonic stem-cells, which will take up the DNA and hopefully express the desired genes. (freeonlineresearchpapers.com)
Wild-type (Nrf2(+/+)) mouse embryonic fibroblasts (MEF) produced reactive oxygen species (ROS) at a low level, whereas treatment with Cd significantly increased the ROS production. (cdc.gov)

A knockout mouse , or knock-out mouse , is a genetically modified mouse ( Mus musculus ) in which researchers have inactivated, or " knocked out ", an existing gene by replacing it or disrupting it with an artificial piece of DNA . (wikipedia.org)
By causing a specific gene to be inactive in the mouse, and observing any differences from normal behaviour or physiology, researchers can infer its probable function. (wikipedia.org)
Consequently, observing the characteristics of knockout mice gives researchers information that can be used to better understand how a similar gene may cause or contribute to disease in humans. (wikipedia.org)
The researchers first tried creating chimeric monkeys using the process for chimeric mice. (livescience.com)
Researchers can insert new genes into these or knock out existing genes with great precision. (newscientist.com)
The researchers isolated cells from the knockout mice and tested the cells' reactions to taxanes and two other anticancer drugs, vincristine and Ara-C. Compared to cells from normal mice that still possessed the gene for Abcc10, the knockout mouse cells were much more sensitive to the drugs. (news-medical.net)
Even though the mice do not have a well-defined macula (a region of the retina that is richer in cones than other parts of the retina), Kellogg researchers hope that this mouse model will allow them to identify the molecules needed for cone function and survival. (sciencedaily.com)
As part of the study, researchers also examined the effect of age on cone function in the absence of rods, recording electrical activity in the retina of Nrl knockout mice at different times after birth. (sciencedaily.com)
The research community has enthusiastically received this technology, and over 1,100 nonprofit researchers thus far have received the materials. (federallabs.org)
Sadayappan along with researchers at UC College of Medicine, Florida State University, the University of Massachusetts Medical School and the Illinois Institute of Technology published research in the scholarly journal PNAS to further the understanding of the protein in skeletal function and regulation. (uc.edu)
Song says that research examined the role of the protein in fast-twitch muscles by generating a knockout mouse - an animal in which researchers have either inactivated, replaced or disrupted the existing fast myosin binding protein-C gene to study its impact. (uc.edu)
In it, the researchers announce that the presence of the Bmal1 gene in the striatum affects alcohol consumption in both male and female mice - but in a sexually dimorphic manner. (concordia.ca)
Expanding on this - and given evidence of sex differences in alcohol consumption and in some functions of clock genes - the researchers hypothesized that Bmal1 may affect alcohol intake in a sex-dependent manner. (concordia.ca)
The researchers created two lines of mice, using molecular biology methods to delete or "knock out" the Bmal1 gene from the striatum's medium spiny neurons in one of them. (concordia.ca)
NEW YORK - Japanese researchers have engineered new variants of the gene-editing enzyme Cas12f that are as effective as the molecular scissors Cas9 but are only one-third of its size. (genomeweb.com)
Although the researchers discovered various potentially effective combinations for engineering an improved AsCas12f gene-editing system, they believe these may not have been the most optimal mixes. (genomeweb.com)

Project 1, Developmental Programming Associated with Maternal Diet and Obesity, research continues on Sub-objective 1A, which examines the impact of maternal high fat diet (HFD) and associated maternal obesity on offspring energy homeostasis, adipose tissue development, and metabolism. (usda.gov)
The Endocrine Unit focuses on metabolic bone diseases and mineral metabolism and ranges from trials of novel therapies for osteoporosis to use of genetically altered mice and cell biology to understand calcium and phosphate metabolism. (massgeneral.org)
Beyond that, we are linking metabolism to the heart and describing phenotypes that emerge in a set of known genes, when knocked out in mice, such as the leptin receptor ( Lepr ), leptin ( Lep ), and Bardet-Biedl syndrome 5 ( Bbs5 ). (springer.com)
Furthermore, we are presenting not yet associated loss-of-function genes affecting both, metabolism and the cardiovascular system, such as the RING finger protein 10 ( Rfn10 ), F-box protein 38 ( Fbxo38 ), and Dipeptidyl peptidase 8 ( Dpp8 ). (springer.com)
Understanding the effect of mouse gene dysfunction on metabolism can inform the functions of their human orthologues. (bvsalud.org)

The phenotype of the L1 mutant mouse indicates that it is a potentially valuable model for the human CRASH syndrome. (jneurosci.org)
Using mice, the International Mouse Phenotyping Consortium (IMPC) aims to target each protein-coding gene and phenotype multiple organ systems in single-gene knockout models by a global network of mouse clinics. (springer.com)

For example, the p53 knockout mouse is named after the p53 gene which codes for a protein that normally suppresses the growth of tumours by arresting cell division and/or inducing apoptosis. (wikipedia.org)
The fact that mice lacking the protein have no obvious health problems is encouraging, suggesting that Abcc10 inhibitors could be used in human patients without causing side effects that might be expected to result from interfering with the pump's normal functions. (news-medical.net)
BV-101 is an adeno- associated virus (AAV) gene therapy vector that simultaneously addresses the metabolic dysfunction of diseased neurons and contributes to the clearance of the mutant huntingtin protein. (ddw-online.com)
To this end, we currently use several (conditional) knock-out mice and cell lines to study gene expression patterns, metabolic profiles, protein interactions, intracellular trafficking, and signaling pathways. (nih.gov)
The X-linked RP3 gene codes for the ciliary protein RPGR and accounts for over 10% of inherited retinal degenerations. (ed.ac.uk)
A team led by Anand Swaroop, Ph.D., professor of ophthalmology and vision research scientist at the University of Michigan Health System's Kellogg Eye Center, has demonstrated that the retinal protein Nrl is required for rod development and, in fact, acts as a "molecular switch," signaling the cells to develop into rods rather than cones. (sciencedaily.com)
The International Mouse Phenotyping Consortium, IMPC, has set the goal of systematically "knocking out" each protein-coding genes in the mouse and phenotyping these so-called knockout (KO) mice using highly standardized high-throughput tests (Brown and Moore 2012 ). (springer.com)
Male mice without the protein consumed more alcohol than those that had it, while female mice without the protein consumed less than females with it. (concordia.ca)
The compact size of the new enzyme variants means that a greater quantity of the gene editing protein can be packaged into vectors such as adeno-associated viruses (AAVs) commonly used to infect living human cells requiring DNA editing. (genomeweb.com)
In the January 18 issue, Small, Beth Stevens at Boston Children's Hospital, and colleagues reported that knocking out a different retromer protein, VPS35, in hippocampal neurons in mice not only jammed neuronal endosome traffic but also caused microglia to assume shapes resembling those seen in AD. (alzforum.org)
Levels of transgene expression in transgenic (Tg) mice expressing deer or elk cellular prion protein (PrP C ). Representative Western blot analysis of PrP C expression from different total protein loads in brain extracts from Tg mice Tg(CerPrP)1536 +/- , Tg(CerPrP-E226)5029 +/- , and Tg(CerPrP-E226)5037 +/- compared with wild type and Prnp 0/0 mice (knock-out mice for PrP gene). (cdc.gov)
TCDD for every step of the mechanism described for 2,3,7,8-TCDD carcinogenesis in humans including receptor binding, gene expression, protein activity changes, cellular replication, oxidative stress, promotion in initiation-promotion studies and complete carcinogenesis in laboratory animals. (who.int)

Mice are currently the laboratory animal species most closely related to humans for which the knockout technique can easily be applied. (wikipedia.org)
Humans share many genes with mice. (wikipedia.org)
This research by itself should help scientists in conducting biomedical research more relevant to humans, he said. (livescience.com)
A method of correcting these defective genes (gene therapy) is being developed in animals with hopes that prevention or a cure for humans will result. (uwm.edu)
In humans, the STAT3 gene is located on the q arm of chromosome 17. (thermofisher.com)
A striking number of cardiovascular defects of humans are modeled in mice with mutation(s) in one or more genes encoding TGFβ ligands. (sc.edu)
Animal models have many facets that mimic various disease conditions in humans representing the importance for its tremendous use in biomedical research (Mukherjee et al. (springer.com)

The pandemic might have affected the number of clinical trials taking place for cell and gene therapies, with activity decreasing by around 15% compared to 2020, but major therapies such as Novartis' gene therapy drug for spinal muscular atrophy (SMA) Zolgensma still made it to market. (ddw-online.com)

Here are this year's winners: Bioscience: Martin Evans, director of the school of biosciences and professor of mammalian genetics at Cardiff University, for his work in stem-cell research and the development of "knockout" mice. (lirneasia.net)

White heterozygous mice can subsequently be crossed to produce mice that are homozygous for the knocked out gene. (wikipedia.org)
This causes the genes to become homozygous (same strain) at nearly all the loci on the chromosomes. (uwm.edu)
Intriguingly, LTP was normal in TEC slices from heterozygous knockouts of the alternate VPS26a subunit, whereas homozygous VPS26a knockouts died in utero. (alzforum.org)

Transgenic mice that were created that had a mutation in the gene which codes for the transcription factor (NGFIB). (freeonlineresearchpapers.com)

Here, we present a novel, statistically robust approach that forges links between 148 MR-associated CNVs and phenotypes from approximately 5,000 mouse gene knockout experiments. (ed.ac.uk)
Millions of knockout mice are used in experiments each year. (wikipedia.org)
To address these points we aim to conduct feeding experiments in young and old mice fed with adequate or suboptimal amounts of six essential TE, including Cu, Fe, I, Mn, Se, and Zn. (uni-potsdam.de)
First transgenic experiments were carried out on mice. (freeonlineresearchpapers.com)
Their experiments in lab mice and in human blood and tissue samples further showed that this gene not only is associated with the disease, as predicted computationally, but is also likely to play a major causal role. (stanford.edu)
Subsequent experiments in mice using the new enzymes, packaged with partner genes in an AAV, confirmed efficient knock-in and knockout effects. (genomeweb.com)
When we think of biology research, we usually think of traditional "wet" labs where experiments are performed and data are generated. (jax.org)

Advances followed that allowed scientists to manipulate and add genes to a variety of different organisms and induce a range of different effects. (wikipedia.org)
But scientists have not managed to isolate such cells from farm animals, and must rely instead on injecting genes randomly into early embryos. (newscientist.com)
To reach this goal, scientists from the Universities of Regensburg and Munich as well as experts in TSPO research from Göttingen and Paris, who have already worked together in the field, will take part in this endeavor. (uni-regensburg.de)
Since discovery of genes scientists have been researching a way to temper with them to achieve a desirable effect. (freeonlineresearchpapers.com)
In searching for risk-increasing genes over the past 10 years, scientists have used two approaches to hunt them down. (stanford.edu)
In this study, the Stanford scientists wanted to know which genes showed especially marked changes in activity, as indicated in earlier comparisons of diabetic vs. healthy tissue samples (notably fat, muscle, liver and beta cells, the only cells in the body that release insulin). (stanford.edu)
To probe VPS26b's role in brain function, the scientists turned to VPS26b knockout mice. (alzforum.org)

Latest achievements involve production of medically important proteins like anti-clotting factors through expression of the gene in lactating cows, goats and pigs. (freeonlineresearchpapers.com)
What does change dynamically, from one tissue or state to another, is what all those genes are doing: how actively each of them is involved in cranking out the starting materials for the many thousands of proteins critical to each cell's or tissue's identity and to every organism's survival. (stanford.edu)
By using knockout mice specifically in brown adipose tissue, this project aims at dissecting the role of Suv420h proteins in the etiology of obesity. (europa.eu)
We could identify 16 proteins which differed in knockout and wild type mice. (lu.se)

Knockout mice also offer a biological and scientific context in which drugs and other therapies can be developed and tested. (wikipedia.org)
This year's event will cover some of the most exciting advancements in areas spanning screening, automation, high content imaging, disease models, cell and gene therapies and how innovation is being driven through partnerships and collaborations. (ddw-online.com)
There's no question that cell and gene therapies have emerged as some of the most promising and innovative medicines on the market. (ddw-online.com)
As it stands, there are currently 19 gene therapies approved for clinical use, alongside 18 RNA therapies and 59 non-genetically modified cell therapies, according to the Q2 report by the American Society of Cell & Gene Therapy. (ddw-online.com)
The Cell and Gene Therapy Catapult alongside ATMP developer Achilles Therapeutics has launched a consortium to develop a smart bioprocessing manufacturing platform for personalised cell therapies. (ddw-online.com)
They also used the enhanced gene editors on human cell lines, which demonstrated their potential for in vivo gene therapies, especially for diseases such as hemophilia, they noted. (genomeweb.com)
Mice models are essential tools to study the pathogenesis of infectious diseases and for the preclinical evaluation of vaccines and therapies against various human pathogens. (medgadget.com)
Mice models are an essential resource for studying the mechanisms underlying infectious disease pathogenesis and as platforms for testing potential vaccines and therapies. (medgadget.com)

CNV screening in clinical practice is limited because pathological CNVs cannot be distinguished routinely from benign CNVs, and because genes underlying patients' phenotypes remain largely unknown. (ed.ac.uk)
Additional enrichments highlighted correspondences between relevant mouse phenotypes and secondary presentations such as brain abnormality, cleft palate, and seizures. (ed.ac.uk)
30% increases) and allowed the identification of 78 genes that may contribute to MR and associated phenotypes. (ed.ac.uk)
In this proposed project, we aimed at studying their in vivo influences on transmitter/receptor phenotypes using mutant mice whose genes of neurotrophins or related molecules are disrupted. (nii.ac.jp)
Sexual Dimorphism of the Mouse Plasma Metabolome Is Associated with Phenotypes of 30 Gene Knockout Lines. (bvsalud.org)
The association of plasma metabolites with IMPC phenotypes showed dramatic sexual dimorphism in wildtype mice . (bvsalud.org)

To test the hypothesis that the precise length of the linker region is not critical for function, we evaluated whether adeno-associated virus-mediated replacement gene therapy with a human ORF15 variant containing in-frame shortening of the linker region could reconstitute RPGR function in vivo. (ed.ac.uk)
Additionally, adeno-associated virus-mediated gene delivery is a safe and effective method for modulating gene expression, and thus is a useful research tool for manipulating individual UPR markers in affected retinas and a promising delivery vector for gene therapy in retinal degenerative disorders. (molvis.org)

Procurement and maintenance of transgenic and knock-out mouse models of human diseases where such animals are available elsewhere without restriction and provide facility and expertise to use the same in drug screening. (ccmb.res.in)
Breeding of currently available transgenic and knock-out mouse models. (ccmb.res.in)
A knock-out mouse model similar to the mut0 human form of methylmalonic acidemia has been developed. (medscape.com)

These extensive high-throughput data from IMPC mice provide a promising opportunity to explore genetics causing metabolic heart disease with an important translational approach. (springer.com)

Potential differences between young and old WT and Nrf2-KO mice may provide an explanation for age-specific TE profiles. (uni-potsdam.de)
This latter method flags differences in genes' activity levels, for example in diseased vs. normal tissues, for each of the 20,000 genes in the entire genome. (stanford.edu)
Yet, the mouse shares a similar developmental trajectory to the human heart and a comparable four-chamber morphology that greatly enhances its translational value as a model organism, despite the substantial differences in size (Krishnan et al. (springer.com)
The sex differences you see in normal mice are eliminated when the gene is taken out of the striatum. (concordia.ca)
Our computational biology work investigates the precise makeup of both mouse and human genomes and the similarities (and important differences) important for translating preclinical research to clinical benefit. (jax.org)
To test the hypothesis that neural gene expression differences underlie differential capacities to bond, we performed RNA-sequencing on tissue from three brain regions important for bonding and other social behaviors across bond-forming prairie voles and non-bonding meadow voles. (cdc.gov)

The enhanced efficacy and exquisite specificity of SELECTIV has broad utility in development of new mouse model systems and expands the use of AAV for gene delivery in vivo. (nature.com)
To complement the in vivo system of chick embryos, we employ patient omics data, single cell RNA sequencing, human and mouse embryos, human cancer cell cultures, human pluripotent stem cells, chick embryo neural crest-derived in vitro crestosphere cultures, and zebrafish and mouse in vivo models. (lu.se)

This produces offspring that are either white and heterozygous for the knocked out gene or grey and wildtype. (wikipedia.org)

In order to identify genes that may modify disease onset and progression, genome-wide association and gene expression studies have been performed 12 , 13 . (nature.com)

A discrepancy in the metabolic pathway of the organism would indicate that a gene coding for a "missing factor" isn't functioning thus will allow its isolation and identification. (freeonlineresearchpapers.com)
Pharmacogenomic studies focusing on candidate gene polymorphisms implicated in antidepressant response, especially in the serotonergic pathway. (researchsquare.com)
In paper №2, I describe how mesDA neurons transplanted in the adult SN of a PD mouse model, extended axons across millimetres into the striatum, functionally reforming the nigrostriatal pathway. (lu.se)

They are important animal models for studying the role of genes which have been sequenced but whose functions have not been determined. (wikipedia.org)
[3] Many mouse models are named after the gene that has been inactivated. (wikipedia.org)
Other mouse models are named according to their physical characteristics or behaviours. (wikipedia.org)
Development of new transgenic and gene knock-out mice models of human diseases. (ccmb.res.in)
The development of transgenic mouse models that express genes of interest in specific cell types has transformed our understanding of basic biology and disease. (nature.com)
Future research will aim at identifying specific inhibitors and/or inducers of UPR regulatory markers as well as expand the list of UPR-related animal models. (molvis.org)
Our laboratory uses molecular, biochemical, physiological, pharmacologic (small molecule inhibitors), cell and tissue culture, and combined optical and biomechanical engineering approaches to analyze cell/tissue/organ-specific conditional/inducible gene knockout and transgenic mouse models. (sc.edu)
Experimental rodent models play a central role in cardiovascular disease research by effectively simulating human cardiovascular diseases. (springer.com)
We are also analyzing whole-genome structure, identifying gene networks and understanding gene expression and its regulation, constructing computational models from genomic data so that function and dysfunction can be predicted based on established patterns, and more. (jax.org)
Mice models are necessary for learning about infections from many human pathogens. (medgadget.com)
Aside from coronavirus studies, mice models are considered the best small animal models for hepatitis B virus (HBV), hepatitis C virus (HCV), Zika virus, and cytomegalovirus (CMV). (medgadget.com)
Mice models have been widely used for various viral studies due to their small size, low cost, ease of use, and high reproducibility. (medgadget.com)
Work in knockout mouse models has shown that TET2 loss enhances hematopoietic stem cell (HSC) self-renewal, expanding the HSC pool (reviewed in refs. (aacrjournals.org)
Investigations in two IGSF1 knockout mouse models converged to show that IGSF1 deficiency leads to reduced expression of the receptor for thyrotropin-releasing hormone (TRH) and impaired TRH stimulation of thyrotropin secretion, providing a candidate mechanism for the central hypothyroidism observed in patients. (medscape.com)
Although early studies of IGSF1 ran into roadblocks and blind alleys, armed with the results of detailed clinical investigations, powerful mouse models, and new reagents, the field is now poised to discover IGSF1's function in endocrine tissues, including the pituitary and testes. (medscape.com)

It allows users to obtain, visualize and prioritize molecular interaction networks using HD-relevant gene expression, phenotypic and other types of data obtained from human samples or model organisms. (nature.com)
Surprisingly, animals deficient for BOB.1 / OBF.1 showed normal expression of genes that contain an octamer motif in their regulatory regions. (nih.gov)
STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. (thermofisher.com)
It involves introduction of a mutation into the "unknown" gene which will suppress its expression. (freeonlineresearchpapers.com)
This research often requires the tissue-specific expression of transgenes to better mirror human diseases, to visualize pathways being studied or to modulate the expression of a disease-related gene. (nature.com)
Transgene expression using viral vectors provides an alternative, which allows for temporal expression of genes in mice with reduced cost and effort. (nature.com)
Over the years, his laboratory has shown that Nrl is a key molecule, which controls the expression of rod-specific genes, including rhodopsin, the visual pigment contained in rods. (sciencedaily.com)
Manipulation of UPR markers has been made possible by the use of knockout mice, pharmacological agents, and viral vector-mediated augmentation of gene expression. (molvis.org)
In this investigation, we considered the effect of PrP gene knock-out (PrP0/0) on cerebellar neural circuits and in particular on granule cells, which show intense PrP expression during development and selective affinity for PrP. (amrita.edu)
At the third postnatal week, when PrP expression would normally attain mature levels, PrP0/0 mice showed low performance in the accelerating rotarod and runway tests and the functioning of 40% of granule cells was abnormal. (amrita.edu)
In addition, an excess of oxidative free radicals also increases the expression of proinflammatory genes and activates the inflammation process [ 18 , 19 ]. (hindawi.com)
With such an approach, a gene of interest and its promoter could be readily humanized in order to reproduce a human expression pattern in the mouse. (genoway.com)
Loss of TET2 has been shown to lead to aberrant gene expression that can disrupt normal differentiation processes. (aacrjournals.org)
Induction of the genes required antioxidant response elements (ARE) as Cd drove ARE-dependent reporter expression and Cd-activated Nrf2 bound to endogenous AREs in mouse hepa1c1c7 cells. (cdc.gov)
Berry intake changes hepatic gene expression and DNA methylation patterns associated with high-fat diet. (lu.se)
Whole genome analysis of lung, sampled 24 hours after challenge, showed expression changes of not only genes belonging to several Gene Ontology groups with up-regulation of inflammatory-associated genes and those associated with lung remodeling but also genes involved in downsizing these processes. (cdc.gov)
Unsensitized animals challenged with TMA exhibited also an increased number of macrophages in BAL, but gene expression in the above-mentioned gene pathways was unchanged or down-regulated. (cdc.gov)

We delivered human RPGR-ORF15 replacement genes with deletion of most (314 codons, 'short form') or 1/3 (126 codons, 'long form') of the linker region to Rpgr null mice. (ed.ac.uk)
Amir notes that neither the sugar consumption nor circadian rhythms is affected by the deletion of the gene. (concordia.ca)

A laboratory mouse in which a gene affecting hair growth has been knocked out (left) is shown next to a normal lab mouse. (wikipedia.org)
More laboratory mice are used in research every year than any other animal species. (uwm.edu)
The laboratory continues to use the technology in research on gene regulation and initiation of transcription and translation, and it has been the subject of over 125 publications by both the inventors and outside investigators. (federallabs.org)
1Current affiliation: Medical Research Council Laboratory of Molecular Biology, Cambridge, UK. (cdc.gov)

We aim to identify new Nrf2-dependent genes relevant for TE homeostasis and intestinal TE absorp-tion both in young and old mice. (uni-potsdam.de)
To identify the Nrf2-modulated part of TE effects, Nrf2 knockout (KO) mice will be studied in parallel. (uni-potsdam.de)
Nrf2 activates transcription of antioxidant enzymes by binding to the antioxidant response element (ARE) in the promoter regions of its target genes [ 23 , 24 ]. (hindawi.com)
Upon exposure to ROS, Keap1 undergoes a conformational change that allows Nrf2 to translocate to the nucleus, bind to the ARE region, and initiate transcription of target genes [ 24 ]. (hindawi.com)
Oxidized Keap1 dissociates from Nrf2, allowing Nrf2 to translocate to the nucleus, bind to the ARE region, and initiate transcription of target genes. (hindawi.com)
On the other hand, Nrf2 knockout (Nrf2(-/-)) MEF cells exhibited an elevated level of ROS under a basal condition, and Cd dramatically increased the ROS production at concentrations as low as 2 mu M, resulting in increased sensitivity to Cd-induced cell death. (cdc.gov)

Blastocysts containing cells, that are both wildtype and knockout cells, are injected into the uterus of a foster mother. (wikipedia.org)
The chimera mice are crossed with a normal wildtype mouse (grey). (wikipedia.org)
Steroids , bile acids , oxylipins , primary metabolites, biogenic amines and complex lipids were analyzed with dedicated mass spectrometry platforms, yielding 827 identified metabolites in male and female KO mice and wildtype (WT) controls. (bvsalud.org)

Therefore, the certain variants in the serotonin-related genes, such as serotonin transporter (5-HTT), 5-HT1A receptor (5-HTR1A), 5-HT2A receptor (5HTR2A) and catechol-O-methyltransferase (COMT) genes, may influence 5-HT neurotransmission, and they are good candidates for the study of PD. (researchsquare.com)
Knocking it out in mice slowed glutamate receptor recycling and weakened synaptic transmission, but only in the transentorhinal cortex, hinting at why this region is so vulnerable to AD pathology in people. (alzforum.org)

In contrast, 12-month-old knockouts had less metabolic activity than controls in the same region. (alzforum.org)
Given the metabolic decline in the 12-month-old VPS26b knockouts, did synaptic signaling wane as well? (alzforum.org)

Since then, testicular maldescent has been the subject of many clinical studies, but its embryology, effects on fertility, and ultimate clinical impact remain topics of discussion and research. (medscape.com)
The four tracks are organized around research themes, and faculty members in each track have corresponding research interests, but are drawn from multiple basic science and clinical departments within the College of Medicine and the entire University of Toledo. (utoledo.edu)
Abundant evidence has shown that serotonin-related gene polymorphisms played an important role in the clinical effects of antidepressants. (researchsquare.com)
This research will contribute to provide new clinical targets against obesity. (europa.eu)
He discovered the indenoisoquinolines as novel Top1 inhibitors, which are in clinical development, and the mitochondrial topoisomerase gene, TOP1mt. (cancer.gov)
As Chief, Dr. Pommier oversees the Branch's clinical/translational research program, which emphasizes new approaches to cancer treatments targeting DNA, epigenetic and chromatin, and connected biomarkers. (cancer.gov)

Knowing the base pair composition of the genome is only half the task done, the other half would be the identification of gene function. (freeonlineresearchpapers.com)
The genome with inactivated gene is introduced into embryo and then selected for. (freeonlineresearchpapers.com)
By applying this powerful approach to other Cas enzymes with different PAM sequences, we can potentially generate efficient genome-editing enzymes capable of targeting a wide range of genes," they concluded. (genomeweb.com)
RMCE technology allows the substitution of large genomic regions in the mouse genome with the concomitant human genomic regions. (genoway.com)
Manipulating the Mouse Genome to Engineer Precise Functional Syntenic Replacements with Human Sequence. (genoway.com)

A great advantage over human studies is the availability of murine tissue samples, in which effects of different TE diets will be studied in close collaboration with all experimental groups of the Research Unit. (uni-potsdam.de)
To date, most research has centered on rods, in part because they make up almost 95% of the photoreceptors in human retina. (sciencedaily.com)
This led to the creation of "knockout" mice, which are used to model human diseases by deactivating a specific gene. (lirneasia.net)
Our research is trying to figure out this problem in human health. (uc.edu)
Mice with xenografted human immune systems have been used to study the pathogenesis of various infectious agents, including Plasmodium falciparum (malaria), Mycobacterium tuberculosis, dengue virus, and influenza virus. (medgadget.com)

Their mutagenic potential and gene regulatory effect have shaped the evolution of transcriptional networks involved in development, pluripotency, and inflammation. (lu.se)

Gene knockout in rats is much harder and has only been possible since 2003. (wikipedia.org)
Carcinogenicity and chronic toxicity in rats and mice exposed to chloroform by inhalation. (who.int)

Working with Swaroop, research investigator Alan J. Mears, Ph.D., deleted the gene that makes Nrl in mice, creating an Nrl-knockout strain that developed a retina without rod photoreceptors. (sciencedaily.com)

I'd like to stress that we did this work in a mouse model,' Hopper-Borge says. (news-medical.net)
North America held the largest share in the mice model market. (medgadget.com)
Moreover, increase in preclinical activities by various pharmaceutical and contract research organizations in the region are further expected to contribute to the growth of the mice model market during the forecast period. (medgadget.com)
Thus, the growing number of infectious disease is driving the mice model market. (medgadget.com)
The authors used a conditional TET2 knockout model to demonstrate that loss of TET2 in either the HSC or early B-cell stage led to enrichment of GC B cells. (aacrjournals.org)
[ 16 ] This model may facilitate further research into the pathophysiology of the disease and broaden its therapeutic options. (medscape.com)
In paper №1, I detail the neurodegenerative and behavioural outcomes in a mouse lesion model of PD, which can be used as a platform for the development of novel therapeutic strategies. (lu.se)

Superior specificity is achieved by combining Cre-mediated AAVR overexpression with whole-body knockout of endogenous Aavr , which is demonstrated in heart cardiomyocytes, liver hepatocytes and cholinergic neurons. (nature.com)

By the natural process of homologous recombination some of the electroporated stem cells will incorporate the new sequence with the knocked-out gene into their chromosomes in place of the original gene. (wikipedia.org)

More importantly, biotechnologists will for the first time be able to manipulate the genes of cells from farm animals directly before growing them into embryos. (newscientist.com)

Shortly after 6 weeks' gestation, the testis-determining SRY gene on chromosome Y directly affects the differentiation of the indifferent gonad into a testis. (medscape.com)

Usually, the new sequence is also given a marker gene , a gene that normal mice don't have and that confers resistance to a certain toxic agent (e.g., neomycin) or that produces an observable change (e.g. colour or fluorescence). (wikipedia.org)
As expected, knockout mice treated with paclitaxel had smaller spleens and thymus glands and underdeveloped bone marrow, compared to normal mice treated with the same drug. (news-medical.net)

They are used to study the diseases associated with defective genes. (uwm.edu)
These mice have deficiencies in their immune systems and thereby prove valuable in the study of immune diseases and cancer. (uwm.edu)
The large number of genes and the diversity of processes involved in the progression of neurological diseases in general, and HD in specific, emphasizes the need for comprehensive approaches in additional to studies of individual genes 14 . (nature.com)
1 Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Südufer 10, 17493 Greifswald - Isle of Riems, Germany. (nih.gov)

In the motor, somatosensory, and visual cortex, many pyramidal neurons in layer V exhibited undulating apical dendrites that did not reach layer I. The hippocampus of L1 mutant mice was smaller than normal, with fewer pyramidal and granule cells. (jneurosci.org)
Enlarged ventricles and septal abnormalities were also features of the mutant mouse brain. (jneurosci.org)
Based on our previous friendship in Cold Spring Harbor Lovoratory, we have established this collaboration project with its neuroscience group to examine a large variety of these mutant mice. (nii.ac.jp)
These unique mutant cell lines may then be used to create unique mutant mouse lines. (genoway.com)
Because multiple mutant cell and mouse lines can be created from one parental ES clonal line, RMCE is cost effective and reliable. (genoway.com)

Mouse VPS26b knockouts had more Aβ, less entorhinal cortex neurotransmission, and worse memory function. (alzforum.org)

The first recorded knockout mouse was created by Mario R. Capecchi , Martin Evans , and Oliver Smithies in 1989, for which they were awarded the 2007 Nobel Prize in Physiology or Medicine . (wikipedia.org)

Therefore, the planned research group aims to characterize the role of TSPO as a diagnostic and therapeutic target from structural biology over neurology to psychiatry and to delineate the potential of TSPO ligands as a novel therapeutic approach within the nervous system. (uni-regensburg.de)
In the present paper, the authors studied the transmission of a C. felis-like parasite in bobcats to domestic cats via infection in mice in order to better understand the biology and interaction of these parasites in the cat. (usda.gov)

The first genetically modified animal was a mouse created in 1974 by Rudolf Jaenisch. (wikipedia.org)

Despite considerable efforts, deciphering the precise pathological mechanisms underlying HD still requires further research. (nature.com)
The purchase of this research is to determine the mechanisms and factors that establish and maintain tubular diameter, to understand the pathobiology of polycystic kidney disease (PKD) and to use this information to find a therapy for PKD. (nih.gov)
We study the mechanisms responsible for the tubes becoming cystic using genetically engineered mice that have PKD and cell lines that mimic some of the characteristics of PKD. (nih.gov)
My research focus is on gaining an understanding of the unique and redundant biological function and properties of the three transforming growth factor beta (TGFβ) ligands and their signaling mechanisms in cardiovascular development and homeostasis and in disease pathogenesis. (sc.edu)
Decades of research on monogamous prairie voles (Microtus ochrogaster), along with comparative studies using the related non-bonding meadow vole (M. pennsylvanicus), have revealed many of the neural and molecular mechanisms necessary for pair-bond formation in that species. (cdc.gov)

The findings appear in the May 16, 2011 issue of the journal Cancer Research . (news-medical.net)
Jan 21, 2003 - Tues 12:30PM - Dr. Jack Taylor, NIEHS - Epidemiologic studies of DNA repair gene polymorphisms and cancer risk Dec 17, 2002 - Dr. John Tainer, UC Berkeley - Conformational Controls and DNA Repair Coordination - [Note: The posting of this talk will be delayed at the request of the speaker. (bio.net)
Senior Scientist and Canada Research Chair, Princess Margaret Cancer Centre, University Health Network. (lu.se)
Co-Director, Translational Research Initiative in Leukemia, Ontario Institute for Cancer Research. (lu.se)

The trial will see Bayer using its subsidiary BrainVectis to investigate its gene therapy, BV-101 in the treatment for Huntington's Disease (HD). (ddw-online.com)
In the UK, effort has been made to scale-up the country's cell and gene therapy manufacturing capabilities. (ddw-online.com)
The critical RPGR-ORF15 splice variant contains a highly repetitive purine-rich linker region that renders it unstable and difficult to adapt for gene therapy. (ed.ac.uk)
If we learn more about this process, we may be able to pinpoint ways to intervene with gene or drug therapy to treat certain types of vision loss. (sciencedaily.com)

This study is the first to demonstrate how the power of mouse knockout data can be systematically exploited to better understand genetically heterogeneous neurological disorders. (ed.ac.uk)

To facilitate research on HD in a network-oriented manner, we have developed HDNetDB, a database that integrates molecular interactions with many HD-relevant datasets. (nature.com)

Function of the gene can be found by "turning it off" and studying the effect on the organism. (freeonlineresearchpapers.com)

European societal needs require desperately a shift forward in the field of obesity research to be able to build new therapeutics strategies. (europa.eu)

Anatomy1

Organisms2

Chemicals and Drugs1

Analytical, Diagnostic and Therapeutic Techniques and Equipment4

Psychiatry and Psychology1

Phenomena and Processes3

Disciplines and Occupations12

Anthropology, Education, Sociology and Social Phenomena1

Technology, Industry, Agriculture1

Humanities1

Information Science2

Named Groups1

Health Care7

Geographicals1

Genetic11

Mutations12

Conditional knockout mice1

Strains7

Embryonic8

Researchers16

Metabolism5

Phenotype2

Protein12

Humans7

20201

Mammalian1

Homozygous3

Transgenic mice1

Experiments7

Scientists7

Proteins4

Therapies8

Phenotypes6

Adeno-associated vir2

Knock-out m3

Genetics1

Differences6

Vivo2

Heterozygous1

Identify genes1

Pathway3

Models15

Expression17

Deletion2

Laboratory4

Nrf26

Wildtype3

Receptor2

Metabolic2

Clinical6

Genome5

Human5

Inflammation1

Rats2

Strain1

Model7

Endogenous1

Homologous recombination1

Embryos1

Chromosome1

Normal mice2

Diseases4

Mutant5

Neurotransmission1

Physiology1

Biology2

Genetically modified1

Mechanisms5

Cancer4

Therapy4

Systematically1

Molecular1

Organism1

Obesity1