• This protein is thought to regulate genes involved in embryogenesis and in myocardial differentiation and function. (wikipedia.org)
  • Han Y , He X , Lee S , Mao Y , Liu X , Sun H , Jin M , Kwon T , . Network analysis for the identification of hub genes and related molecules as potential biomarkers associated with the differentiation of bone marrow-derived stem cells into hepatocytes. (aging-us.com)
  • Herein, we screened differentially expressed genes between hepatocytes and untreated BMSCs to identify the genes responsible for the differentiation of BMSCs into hepatocytes. (aging-us.com)
  • Hub genes were analyzed using the STRING and Cytoscape v 3.8.2, revealing that twenty-four hub genes, play a pivotal role in the differentiation of BMSCs into hepatocytes. (aging-us.com)
  • Several other environmental factors including oxy- gen concentration and mechanical, metabolic, and bio- chemical conditions have been shown relevant in cell differentiation and have been reviewed extensively (Fig. 1) [3]. (unicyte.ch)
  • Their proliferation and fusion leads to division of the ventricular inlet into two different passageways with two AV valves, and they are thought to be under the influence of the GATA4 transcription factor. (wikipedia.org)
  • The final result is that, after MI, billions of cardiomyocytes (CMs) with limited proliferation capacity are lost and substituted by heterogeneous collagen-rich fibrotic scar tissue [ 2 ] [ 3 ] . (encyclopedia.pub)
  • Ang II binds to AT 1 R, which can induce vasoconstriction, increase blood pressure, and further induce cardiac hypertrophy and proliferation of fibroblasts [15]. (researchsquare.com)
  • There is a growing body of evidence which demonstrates that following ACS, microRNAs might inhibit fibroblast proliferation and scarring, as well as harmful apoptosis of cardiomyocytes, and stimulate fibroblast reprogramming into induced cardiac progenitor cells. (archivesofmedicalscience.com)
  • Myocardial infarction (MI) is one of the clusters of numerous other cardiovascular diseases and occurs due to the blockage of the coronary artery delivering blood (ischemia) to the ventricle with consequent oxygen shortage to the contractile cells (cardiomyocytes) [ 1 ] . (encyclopedia.pub)
  • Myocardial hypertrophy occurs in many cardiovascular diseases. (researchsquare.com)
  • Myocardial hypertrophy is one of the important causes of death of patients caused by cardiovascular diseases and an independent risk factor for the morbidity and mortality of cardiovascular diseases [1-3]. (researchsquare.com)
  • Previous studies have shown that its development is closely related to cardiac overload[6], so abdominal aortic constriction(AAC) can use this principle to establish model of myocardial hypertrophy. (researchsquare.com)
  • The rennin-angiotensin system (RAS) has been recognized to be an important factor in myocardial hypertrophy[8]. (researchsquare.com)
  • AT 1 R is a member of the 7-fold transmembrane G-protein-coupled receptor (GPCR) family[12,13], mainly exists in the myocardial membrane and can mediate cardiac hypertrophy induced by pressure overload and mechanical stimulation after activation [14]. (researchsquare.com)
  • Compounds that target tissue-specific transcription factors could overcome non-specific effects of small molecules and lead to the regeneration of heart muscle following myocardial infarction. (biomedcentral.com)
  • WNT signaling plays an important role in cardiac development, but abnormal activity is often associated with cardiac hypertrophy, myocardial infarction, remodeling, and heart failure. (kjpp.net)
  • Mutations in this gene have been associated with cardiac septal defects as well as reproductive defects. (wikipedia.org)
  • Mutations or defects in the GATA4 gene can lead to a variety of cardiac problems including congenital heart disease, abnormal ventral folding, and defects in the cardiac septum separating the atria and ventricles, and hypoplasia of the ventricular myocardium. (wikipedia.org)
  • This data could have important implications for human medicine by suggesting that mutations with the GATA4 transcription factor could be responsible for AV cushion defects in humans with improper septal formation leading to congenital heart disease. (wikipedia.org)
  • Novel Point Mutations of CITED2 Gene Are Associated with Non-familial Congenital Heart Disease (CHD) in Sporadic Pediatric Patients. (cdc.gov)
  • Intronic Polymorphisms in Gene of Second Heart Field as Risk Factors for Human Congenital Heart Disease in a Chinese Population. (cdc.gov)
  • Association of NKX2-5, GATA4, and TBX5 polymorphisms with congenital heart disease in Egyptian children. (cdc.gov)
  • Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. (cdc.gov)
  • GATA4 inactivation, with GATA4-null mice, leads to down regulation of Erbb3 and altered Erk expression, two other important molecules in EMT and ventricular inlet separation. (wikipedia.org)
  • Atrial septal defects, tetralogy of Fallot, and ventricular septal defects associated with GATA4 mutation were also seen in South Indian patients. (wikipedia.org)
  • If we allow this to continue, the results may lead to ventricular remodeling, decompensation of cardiac function and dilated cardiomyopathy, heart failure and even sudden cardiac death[7] Therefore, preventing the occurrence and development of cardiac hypertrophy is very important. (researchsquare.com)
  • Due to cellular heterogeneity in the heart, the activation of gene programs representing specific atrial and ventricular cardiomyocyte subtypes would be highly desirable. (biomedcentral.com)
  • Transcription factor GATA4-targeted compounds that have previously shown in vivo efficacy in cardiac injury models were tested for stage-specific activation of atrial and ventricular reporter genes in differentiating pluripotent stem cells using a dual reporter assay. (biomedcentral.com)
  • GATA4-targeted compounds 3i-1000 and 3i-1103 were identified as differential modulators of atrial and ventricular gene expression. (biomedcentral.com)
  • More detailed structure-function analysis revealed a distinct subclass of GATA4/NKX2-5 inhibitory compounds with an acetyl lysine-like domain that contributed to ventricular cells (%Myl2-eGFP+). (biomedcentral.com)
  • A cell-based functional assay system measuring hypertrophic cell growth of neonatal rat ventricle cardiomyocytes (NRVMs) following phenylephrine treatment was applied, and changes in phosphorylation of individual differentially phosphorylated sites were induced by incorporation of phosphorylation competition peptides conjugated with cell-penetrating peptides. (molcells.org)
  • theβ-myosin heavy chain(β-MHC)and atrial natriuretic factor (ANF), which are recognized as a marker of cardiac hypertrophy, were determined by Real-time quantitative PCR (qRT-PCR), then another gene phospholipase C (PLC), inositol triphosphate (IP 3 ), which associated with RAS were determined by Western blot(WB). (researchsquare.com)
  • Absence of GATA4 Mutations in Moroccan Patients with Atrial Septal Defect (ASD) Provides Further Evidence of Limited Involvement of GATA4 in Major Congenital Heart Defects. (cdc.gov)
  • Inhibition of p62 leads to increased GATA4 levels, resulting in NF-kB activation and subsequent SASP induction. (wikipedia.org)
  • Reporter gene assays were conducted to examine combinatorial effects of GATA-targeted compounds and bromodomain and extraterminal domain (BET) inhibition on chamber-specific gene expression. (biomedcentral.com)
  • GATA4 is expressed in both embryo and adult cardiomyocytes where it functions as a transcriptional regulator for many cardiac genes, and also regulates hypertrophic growth of the heart. (wikipedia.org)
  • Collectively, these results indicate the potential for therapeutic alteration of cell fate decisions and pathological gene regulatory networks by GATA4-targeted compounds modulating chamber-specific transcriptional programs in multipotent cardiac progenitor cells and cardiomyocytes. (biomedcentral.com)
  • A pathological cardiac hypertrophy model, junctate-1 transgenic mice and control mice, were analyzed using label-free quantitative phosphoproteomics to identify differentially phosphorylated proteins and sites. (molcells.org)
  • Changes in phosphorylation levels of Ser-98 and Ser-179 in Ldb3 were further confirmed in NRVMs and other pathological/physiological hypertrophy models, including transverse aortic constriction and swimming models, using site-specific phospho-antibodies. (molcells.org)
  • Feric, N. T. & Radisic, M. Maturing human pluripotent stem cell-derived cardiomyocytes in human engineered cardiac tissues. (nature.com)
  • GATA4 is a critical transcription factor for proper mammalian cardiac development and essential for survival of the embryo. (wikipedia.org)
  • GATA4 is not only important for cardiac development, but also development and function of the mammalian fetal ovary and contributes to fetal male gonadal development and mutations may lead to defects in reproductive development. (wikipedia.org)
  • GATA4 expression during cardiac development has been shown to be essential to proper atrioventricular (AV) formation and function. (wikipedia.org)
  • 6% of the ESTs matched neither GenBank cDNA sequences nor the newly assembled chicken genomic DNA sequence, three ESTs aligned with sequences designated to be on the Z_random, while one matched a W chromosome sequence and could be useful in cataloguing functionally important genes on this sex chromosome. (westminster.ac.uk)
  • Mutations in this gene have been associated to cases of congenital diaphragmatic hernia. (wikipedia.org)
  • This indicated the involvement of epigenetic modulators in the regulation of GATA-dependent transcription. (biomedcentral.com)
  • In this review, we focus on the role of cardiomyocyte-derived and cardiac fibroblast-derived microRNAs that are involved in the regulation of genes associated with cardiomyocyte and fibroblast function and in atherosclerosis-related cardiac ischemia. (archivesofmedicalscience.com)
  • A tight spatial and timing regulation of growth factor action during embryonic development has been suggested [4]. (unicyte.ch)
  • GATA4 has also been discovered to have an integral role in controlling the early stages of pancreatic and hepatic development. (wikipedia.org)
  • Finally, the gene expression of Atf4, Ire1α, Xbp1s, Xbp1t, Grp78 and Chop was measured from these cDNA samples by qPCR (quantitative polymerase chain reaction). (helsinki.fi)
  • The expression of the hub genes in the BMSCs and hepatocytes was verified by reverse transcription-quantitative PCR (RT-qPCR). (aging-us.com)
  • Pharmacological modulation of cell fate decisions and developmental gene regulatory networks holds promise for the treatment of heart failure. (biomedcentral.com)
  • In this line, reporter gene assays with combinatorial treatment of 3i-1000 and the BET bromodomain inhibitor (+)-JQ1 demonstrated the cooperative role of GATA4 and BRD4 in the modulation of chamber-specific cardiac gene expression. (biomedcentral.com)
  • In cardiac hypertrophy, phosphorylation-dependent functional modulation of proteins, especially sarcoplasmic reticulum (SR) and sarcomere proteins, has been demonstrated. (molcells.org)
  • As seen from the abnormalities from deletion of GATA4, it is essential for cardiac formation and the survival of the embryo during fetal development. (wikipedia.org)
  • GATA4 promotes cardiac morphogenesis, cardiomyocytes survival, and maintains cardiac function in the adult heart. (wikipedia.org)
  • MicroRNAs regulate gene expression at the post-transcriptional level by binding to 3′- or less often to 5′-untranslated regions of target messenger RNAs (mRNAs), which in consequence leads to inhibited translation and/or induces degradation of targeted mRNA [ 1 ]. (archivesofmedicalscience.com)
  • EP300 - and GATA4 -directed contractile deficits due to mechanical stress. (nature.com)
  • Additionally, BioID analysis indicated broad interaction between GATA4 and BET family of proteins, such as BRD4. (biomedcentral.com)
  • Additionally, single nucleotide polymorphisms (SNPs) were identified and validated in 10 ESTs that showed 98% or higher sequence similarity to known chicken genes. (westminster.ac.uk)
  • The qPCR data describes the expression of exact gene. (helsinki.fi)
  • By statistical analysis, it was possible to compare the expression of these genes between 6-OHDA group and vehicle group. (helsinki.fi)
  • In other genes there were no statistical differences, unlike in several other studies where the expression was found to be increased. (helsinki.fi)
  • Chemically induced gene expression changes were characterized by qRT-PCR, global run-on sequencing (GRO-seq) and immunoblotting, and the network of cooperative proteins of GATA4 and NKX2-5 were further explored by the examination of the GATA4 and NKX2-5 interactome by BioID. (biomedcentral.com)
  • These processes influence gene expression patterns or cellular phenotypes and disease states with no underlying change in DNA sequence. (emjreviews.com)
  • 4 Acute hyperglycaemia leads to chronic metabolic and haemodynamic derangements, 5 which trigger chromatin structural changes, transcription factor activation, and gene expression. (emjreviews.com)
  • GSE30419 gene microarray data of BMSCs and GSE72088 gene microarray data of primary hepatocytes were obtained from the Gene Expression Omnibus database. (aging-us.com)
  • Its importance has been documented in a number of physiologic and pathologic conditions including neuronal and muscle development, lymphocyte activation, cardiac hypertrophy, switching of skeletal muscle fiber type, and expression of ion channels. (pancreapedia.org)
  • Also, the study will look at whether there are gene expression profiles in the tumor tissue that can predict pathologic complete response. (stanford.edu)
  • It is histologically defined by renal glomerular hypertrophy, expansion of mesangial and tubular compartments, accumulation of mesangial extracellular matrix proteins, and podocytopenia associated with foot process effacement. (emjreviews.com)
  • Here, we have described resources that may be useful in comparative and functional genomic analysis of genes expressed in an important organ, the pineal gland, in a model and agriculturally important organism. (westminster.ac.uk)
  • The Functional Polymorphism R129W in the BVES Gene Is Associated with Sporadic Tetralogy of Fallot in the Han Chinese Population. (cdc.gov)
  • GATA4 molecular screening and assessment of environmental risk factors in a Moroccan cohort with tetralogy of Fallot. (cdc.gov)
  • If ER stress is a significant factor that affects the disease development, it would be important to find a drug that alters these mechanisms and UPR. (helsinki.fi)
  • GATA4 is regulated through the autophagy-lysosome pathway in eukaryotic cells. (wikipedia.org)
  • Cells can communicate via physical interactions, in- cluding membrane bridge formation, such as tunneling nanotubes and cytonemes, and/or through the release of soluble factors [1-3]. (unicyte.ch)
  • CN has a number of targets but its most prominent are the NFAT (nuclear factor of activated T cells) transcription factors. (pancreapedia.org)
  • GATA4 has also been shown to interact with Erbb3, FOG-1, and FOG-2. (wikipedia.org)
  • May also function as a paracrine antifibrotic factor in the heart. (cusabio.com)
  • Growth factors may act either in an autocrine or a paracrine fashion and their temporal and spatial concentration modulates the cell phenotype and function. (unicyte.ch)
  • Autonomous beating rate adaptation in human stem cell-derived cardiomyocytes. (nature.com)
  • The fate of the cell is deter- mined by coordinated and dynamic interactions among a number of factors, acting in a defined microenviron- ment. (unicyte.ch)
  • MicroRNAs are small non-coding post-translational biomolecules which, when expressed, modify their target genes. (archivesofmedicalscience.com)
  • Next, the target miRNAs of hub genes were predicted, and then the lncRNAs regulating miRNAs was discovered, thus forming the lncRNA-miRNA-mRNA interaction chain. (aging-us.com)
  • In cellular senescence, ATM and ATR inhibit p62, an autophagy adaptor responsible for selective autophagy of GATA4. (wikipedia.org)
  • Although cardiac hypertrophy is initiated by various receptors at cell membranes sensing biomechanical signals and hormones, it is generally mediated by cellular signaling cascades. (molcells.org)
  • Transcriptome Analysis Console software showed that 1896 genes were upregulated and 2506 were downregulated in hepatocytes as compared with BMSCs. (aging-us.com)
  • DN is a complex multifactorial disease caused by multiple genetic and environmental factors. (emjreviews.com)
  • The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. (cusabio.com)