Genes escherichia coli proteins. Medical search. Frequent questions

Some inversions can drastically impact gene expression , even between different strains of E. coli, and could provide a mechanism for the diversification of genetic content through controlled expression changes. (bvsalud.org)
By comparing the growth patterns of prototypical enteropathogenic, uropathogenic, and nonpathogenic Escherichia coli strains devoid of σ 54 , we uncovered that the absence of σ 54 results in two differently sized colonies that appear at different times specifically in the uropathogenic E. coli (UPEC) strain. (nih.gov)
Shiga toxin-producing Escherichia coli (STEC) is a of STEC strains to invade epithelial cells in vitro, although pathogen that causes gastroenteritis and bloody diarrhea in small numbers ( 2,3 ), but no reports of invasion in vivo but can lead to severe disease, such as hemolytic uremic have been published. (cdc.gov)
Diarrheagenic Escherichia coli strains, particularly protein called intimin. (cdc.gov)
The in vitro and in vivo analysis of the ribonuclease E-deficient (rne - ) and the altered mRNA stability protein-deficient (ams - ) strains of Escherichia coli has demonstrated that they carry mutations in the same structural gene. (psu.edu)
Strains encoding either thermolabile RNase E (rne-3071) or Ams protein (ams-1) are defective in both rRNA processing and mRNA turnover. (psu.edu)
Escherichia coli strains TG1 and BL21 (DE3) were grown at 37°C in LB medium. (hindawi.com)
Presented here is a protocol for the preparation and buffer calibration of cell extracts from exonuclease V knockout strains of Escherichia coli BL21 Rosetta2 ( ΔrecBCD and ΔrecB ). (jove.com)
5] "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110. (tcdb.org)

RNAs that are produced by Dicer act as guides for a sequence of particular silencing of cognate genes through RNAi and related pathways. (wikipedia.org)
In addition, the transient time for the coupled reaction sequence was less for the fusion protein than for the free enzymes. (lu.se)
It does not include the E. coli sequence. (embl-heidelberg.de)
The complete nucleotide sequence of 15,611 bases has been determined, and the gene structures were examined. (embl-heidelberg.de)
Sequence homology searches identify PhnC, PhnK, PhnL, and, possibly, PhnN proteins as members of nucleotide-binding proteins of the binding protein-dependent transport systems. (embl-heidelberg.de)
A Pho box-like promoter sequence is also found upstream of the gene cluster starting at phnA, which is consistent with the observation of phosphate regulation of the Phn+ response. (embl-heidelberg.de)
Nucleotide sequence and expression in Escherichia coli of the gene encoding the nonstructural protein NCVP2 of bovine rotavirus. (microbiologyresearch.org)
Cloning and nucleotide sequence of the simian rotavirus gene 6 that codes for the major inner capsid protein. (microbiologyresearch.org)
Although the protein sequence showed high identity with plant and invertebrate MTs, it contained a unique 'linker ' region (14 amino acid residues) between the two putative metal-binding domains which contained no cysteine residues. (portlandpress.com)
3] "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes. (tcdb.org)
4] "The complete genome sequence of Escherichia coli K-12. (tcdb.org)
8] "Nucleotide sequence of the FNR-regulated fumarase gene (fumB) of Escherichia coli K-12. (tcdb.org)
Previous protein sequence homologies suggested an inner membrane location for FtsE. (ox.ac.uk)
Using DNA extracted from bacteria in the dental plaque of mother/child pairs, a sequence of the S. mutans spaP gene was amplified using PCR. (bvsalud.org)

In addition, an Escherichia coli strain which was deficient in both transaldolases was constructed. (europa.eu)
We present the preparation steps of cell lysate from Escherichia coli BL21 Rosetta2 ΔrecBCD strain by sonication lysis and buffer calibration for Mg-glutamate (Mg-glu) and K-glutamate (K-glu) specifically for linear DNA. (jove.com)
In this protocol, steps for the preparation of cell-free lysates from the E. coli BL21 Rosetta2 ΔrecBCD strain by sonication lysis are described in detail. (jove.com)
The reason for the observed defective assembly in E. coli is probably not some unidentified mutation in the plasmid DNA because the same plasmid preparation complemented a B. subtilis sdhCAB deletion strain [7]. (lu.se)

The identified inversions had more overlap than expected with nucleoid-associated protein binding sites, which assist in the regulation of genomic gene expression . (bvsalud.org)
This finding suggests a role for this σ 54 in gene regulation that extends beyond its known function as an RNAP gene specificity factor. (nih.gov)
Yeast nucleases with the Class 2 RNase III domain: RNT1 (UniProtKB Q02555) - S. cerevisiae - this RNase III is involved in the transcription and processing of rDNA, the 3' end formation of U2 snRNA via cleavage of the terminal loop, cell wall stress response and degradation, and regulation of morphogenesis checkpoint genes. (wikipedia.org)
Predicted to be involved in positive regulation of protein sumoylation and protein sumoylation. (nih.gov)
These results suggest that Esr41 plays important roles in the inverse regulation of LEE and flagellar gene expression. (nih.gov)
KIN17, which is known as a DNA and RNA binding protein, is highly expressed in numerous types of human cancers and was discovered to participate in several vital cell behaviors, including DNA replication, damage repair, regulation of cell cycle and RNA processing. (spandidos-publications.com)
Previous studies have indicated that KIN17 is involved in global genome repair, DNA replication, transcription and regulation of the cell cycle as part of a multi-protein complex. (spandidos-publications.com)
A mathematical model of metabolism and regulation provides a systems-level view of how Escherichia coli responds to oxygen. (mpg.de)
We have investigated potential mechanisms of regulation of rotavirus gene expression by functional evaluation of two differentially expressed viral mRNAs. (microbiologyresearch.org)
Transcriptional regulation of the genes in metabolic pathways is a highly successful strategy, which is virtually universal in microorganisms. (lu.se)
With a minimalist model of metabolism, cell growth and transcriptional regulation in a microorganism, we explore how the interaction between environmental conditions and gene regulation set the growth rate of cells in the phase of exponential growth. (lu.se)
INTRODUCTION broken, which in realistic situations can severly constrain the Transcriptional regulation of effector genes is a highly successful regulatory options. (lu.se)
A very natural place to study gene detecting and metabolizing lactose, it is known that the overall regulation is in the metabolism of the cell, and then specifically in effect of expressing the lac genes in vain is a drop in the growth rate the regulation of genes that code for enzymes and transporter of as much as 5% [1,6]. (lu.se)
Here, the function of regulation is quite clear: expressing energy and carbon, that a number around 0.2% would be the right genes at the right time will enable the cell to make the expected, and that the difference is more or less specific to the lac most of the resources within its reach, by maximizing the uptake operon [7]. (lu.se)
In unicellular organisms like E. coli and yeast, the benefits of between potential cost and benefit, regulation can make the most a well-adapted regulatory system are readily quantified, as the difference to the long-term growth rate if the resource in question fitness of an individual can be estimated by its growth rate in is only available a similarly small fraction of the time. (lu.se)
A number of studies have explored how regulation of generally, and at least to a first approximation, it is obvious that metabolic pathways affects the growth rate of microorganisms, gene regulation only is useful if the environmental conditions vary both in the steady state and in response to changes in the local with time. (lu.se)
The analysis revealed altered transcription levels of 12 E. coli genes in response to phage infection, and the observed regulation of phage genes correlated with the known in vivo pattern of M13 mRNA species. (lu.se)

A gene was identified (1229 bp) with a putative open reading frame (204 bp) encoding a 67-amino-acid protein exhibiting several characteristic features of MT proteins, including 16 cysteine residues (24%) and only one aromatic residue. (portlandpress.com)

Babitzke, P & Kushner, SR 1991, ' The Ams (altered mRNA stability) protein and ribonuclease E are encoded by the same structural gene of Escherichia coli ', Proceedings of the National Academy of Sciences of the United States of America , vol. 88, no. 1, pp. 1-5. (psu.edu)
The mRNAs are extruded into the cytoplasm where they serve as mRNA for protein synthesis and as templates for packaging and replication into dsRNA. (microbiologyresearch.org)
We measured the levels of gene 5 and gene 6 mRNA and showed that they were not significantly different, and protein analysis indicated no difference in stability of NSP1 compared with VP6. (microbiologyresearch.org)
Polyribosome analysis showed that the majority of gene 6 mRNA was present on large polysomes. (microbiologyresearch.org)
In contrast, sedimentation of more than half of the gene 5 mRNA was subpolysomal. (microbiologyresearch.org)
The change in distribution of gene 5 mRNA in polyribosome gradients in response to treatment with low concentrations of cycloheximide suggested that gene 5 is a poor translation initiation template compared with gene 6 mRNA. (microbiologyresearch.org)

This purified protein, which is homologous to human DNA-binding protein YB-1, recognizes some feature of the 110-bp promoter region of hns and acts as a cold shock transcriptional activator of this gene since it stimulates the expression of CAT activity and of cat transcription in in vitro systems programmed with plasmid DNA carrying the hns-cat fusion. (unicam.it)
We compared the survivability of E. coli XL1-Blue cells transformed with a recombinant plasmid containing a glutathione S-transferase (GST)-Oshsp16.9 fusion protein (pGST-FL cells) with the control E. coli cells transformed with the pGEX-2T vector (pGST cells) under heat-shock (HS) after isopropyl β-D-thiogalactopyranoside induction. (ncu.edu.tw)
Five plasmid protein antitoxin-regulated systems and three plasmid antisense RNA-regulated systems were sought by PCR. (who.int)
All these strategies cost extra time and resources and are yet to obtain near-plasmid levels of protein expression. (jove.com)
This method is able to achieve protein expression levels comparable to that from plasmid DNA in E. coli CFPS. (jove.com)
A gene coding for a kappa bungarotoxin (K-Bgt) and rat intestinal fatty acid binding fusion protein was previously constructed in an Escherichia coli plasmid in order to allow synthesis of K-Bgt in vitro. (kzoo.edu)
The operon on plasmid in E. coli was found to result in the production of all three subunit polypeptides [7]. (lu.se)

Biotin-ubiquitin tagging of mammalian proteins in Escherichia coli. (nih.gov)
They are members of the rBAT family of mammalian proteins (TC #8.A.9). (tcdb.org)

Production of perdeuterated proteins for neutron-scattering experiments. (lu.se)

Hence, the genes that rely upon σ 54 for their transcription have a wide range of different functions suggesting that the repertoire of functions performed by genes, directly or indirectly affected by σ 54 , is not yet exhaustive. (nih.gov)
Translational gene-reporter fusion experiments revealed that Esr41 regulates ler expression at a post-transcriptional level, and pch transcription, probably via an unknown target of Esr41. (nih.gov)
In addition, Esr41 activated the expression of flagellar Class 3 genes by indirectly inducing the transcription of fliA, which encodes the sigma factor for flagellar synthesis. (nih.gov)
We implement the controller in an Escherichia coli cell-free transcription-translation system, which allows rapid prototyping and implementation. (nature.com)
The bacterial activator protein NorR binds to enhancer-like elements, upstream of the promoter site, and activates 54-dependent transcription of genes that encode nitric oxide detoxifying enzymes (NorVW), in response to NO stress. (strath.ac.uk)
RIViT-seq technology could speed up associating transcription factors with their target genes. (doe.gov)
Real-time quantitative PCR (RT-qPCR) analysis and sfGFP (superfolder green fluorescent protein) analysis reveal that AmtR directly and negatively regulates the transcription and expression of crn A, cre T, csh A, and hyu B. A growth test shows that C. glutamicum ATCC 14067 can use creatine or creatinine as a sole nitrogen source. (frontiersin.org)
The significantly upregulated genes encode proteins involved in reactions of the energy-generating phosphotransferase system and transcription processing, which could be related to phage transcription. (lu.se)

Of the seven σ factors in Escherichia coli and related bacteria, σ 54 exists in a class of its own. (nih.gov)
The CusB protein is part of the CusCBA periplasmic Cu(I) efflux system in Gram-negative bacteria, and it was recently reported to play a key role in the functioning of the whole CusCBA system, in which conformational changes as well as the assembly/disassembly process control the opening of the transporter. (biorxiv.org)
TolC family proteins are ubiquitous among gram-negative bacteria, and the conserved apertures present a possible chemotherapeutic target in multidrug-resistant pathogens. (umsystem.edu)
Streamlining Regulon Identification in Bacteria Regulons are a group of genes that can be turned on or off by the same regulatory protein. (doe.gov)
Whereas bacteria such as Escherichia coli have been known for some time to cleave carbon-phosphorus (C-P) bonds in unactivated alkylphosphonates, the enzymes responsible for C-P lyase activity have resisted detection or purification. (embl-heidelberg.de)
An association was found between experience of caries in the children and the presence of bacteria carrying the spaP+ gene. (bvsalud.org)
The realization of the vastness of bacterial diversity and the development of novel experimental techniques have propelled molecular microbiology into a new era of investigation This course provides insights into how genes are organized and regulated in bacteria and in their viruses. (lu.se)

TY - JOUR T1 - Structural characterization of Escherichia coli ribosomal protein S8 and its binding site in 16S ribosomal RNA. (unboundmedicine.com)
Isolation and characterization of the Neisseria meningitidis lpxD-fabZ-lpxA gene cluster involved in lipid A biosynthesis. (wikigenes.org)
Characterization of three fimbrial genes, sefABC, of Salmonella enteritidis. (wikigenes.org)

RS_01910 may encode a protein related to creatine transport. (frontiersin.org)
The recent Encyclopedia of studies in the 1950s and 1960s in Escherichia coli and then by DNA Elements (ENCODE) project showed binding of carcinogens to nu- Szybalski (1958) , who tested more indicates that at least 80% of the cleic acids ( Wiest and Heidelberger, than 400 compounds in E. coli . (who.int)

Inversions, in particular, facilitate genome evolution through gene gain and loss, and can alter gene expression . (bvsalud.org)
This fails to encompass a genome -wide perspective of naturally occurring inversions and their post-adaptation impact on gene expression . (bvsalud.org)
The stx genes are located in a bacteriophage fecal samples of a 2-week-old boy who had bacteremia and HUS and in fecal samples of his asymptomatic family integrated into the bacterial genome, and the production members. (cdc.gov)
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. (lbl.gov)
Genome-wide comparison of phage M13-infected and uninfected E. coli, 2 and 20 min after infection, was performed. (lu.se)

One of the functions of the FtsZ ring is to recruit other cell division proteins to the septum to produce a new cell wall between the dividing cells. (joplink.net)
The Escherichia coli cell division proteins FtsY, FtsE and FtsX are inner membrane-associated. (ox.ac.uk)

The cell division genes ftsY, ftsE and ftsX form an operon mapping at 76 min on the Escherichia coli chromosome. (ox.ac.uk)

The experimental part focus on the model organisms, Bacillus subtilis , Escherichia coli and Streptomyces coelicolor . (lu.se)
Why is Bacillus subtilis respiratory Complex II not assembled when produced in Escherichia coli? (lu.se)

When the cell lysates from these two E. coli transformants were heated at 55°C, the amount of protein denatured in the pGST-FL cells was 50% less than that of the pGST cells. (ncu.edu.tw)
METHODS: A collection of 125 TEM, SHV and CTX-M ESBL-producing E. coli isolates and their 125 transconjugants or transformants was analysed. (who.int)

This protein family includes an uncharacterised member designated phnA in Escherichia coli, part of a large operon associated with alkylphosphonate uptake and carbon-phosphorus bond cleavage. (embl-heidelberg.de)
The lac operon of E. coli is but one example of how enzyme and transporter production can be made conditional on the presence of a nutrient to catabolize. (lu.se)
In the case of the lac operon of E. coli, a well-studied system for whether a gene is regulated. (lu.se)
However, the E. coli cells did not show increased succinate dehydrogenase activity nor did the operon complement a sdhCDAB defective E. coli mutant [7]. (lu.se)

Similar results as pGST-FL cells were obtained in pGST-N78 cells (cells produced a fusion protein with only the N- terminal 78 aa in the Oshspl6.9 portion) but not in pGST-C108 cells (cells produced a fusion protein with C-terminal 108 aa in the Oshspl6.9 portion). (ncu.edu.tw)
In this study, the sp2 gene was cloned and expressed in Escherichia coli to produce a 6xHis-tagged fusion protein, which was then used to generate a polyclonal antibody. (hindawi.com)
The fusion protein (CS1/MDH1) is the C-terminus of CS1 linked in-frame to the N-terminus of MDH1 with a short linker of glycyl-seryl-glycyl. (lu.se)
The fusion protein produced was isolated and purified. (lu.se)
This is the expected M(r) for the fusion protein subunit. (lu.se)
The kinetics of CS1 and MDH1 activities of the fusion protein were compared to those of the free enzymes. (lu.se)
It was observed that AAT was a less effective competitor for OAA when the CS1/MDH1 fusion protein is used than when the separate enzymes are employed. (lu.se)
An S-peptide Fucus MT fusion protein expressed in Escherichia coli exhibited a relative molecular mass of ∼ 14 kDa. (portlandpress.com)

Here, we used bioinformatic techniques and multiple RNA-seq datasets to investigate the short- and long-range impact inversions have on genomic gene expression within Escherichia coli . (bvsalud.org)
The Giardia lamblia vsp gene repertoire: characteristics, genomic organization, and evolution. (mbl.edu)
We find that repair of genomic lagging strand mismatches occurs bi-directionally in E. coli and that, while all MutS-recognized mismatches had been thought to be repaired in a consistent manner, the directional bias of repair and the effects of mutations in MutS are dependent on the molecular species of the mismatch. (duke.edu)

Modulations in lipid A and phospholipid biosynthesis pathways influence outer membrane protein assembly in Escherichia coli K-12. (wikigenes.org)
No genes belonging to any known E. coli stress response pathways were scored as upregulated. (lu.se)

The recombinant protein in Escherichia coli lysates was labelled P2 (P2 star) to differentiate it from the comigrating adhesin protein called P2. (bmj.com)
Recently, we showed that linear DNA can be much better protected in lysates from cells knocked out for exonuclease genes ( recBCD ) 10 . (jove.com)

We observed differences in gene expression between homologous inverted and non-inverted genes even after long-term exposure to adaptive selection. (bvsalud.org)
1] "Construction and properties of Escherichia coli mutants defective in two genes encoding homologous membrane proteins with putative roles in anaerobic C4-dicarboxylic acid transport. (tcdb.org)
2] "Escherichia coli possesses two homologous anaerobic C4-dicarboxylate membrane transporters (DcuA and DcuB) distinct from the aerobic dicarboxylate transport system (Dct). (tcdb.org)

A gene encoding the rice 16.9-kDa class I low-molecular-mass (LMM) heat- shock protein (HSP), Oshsp16.9, was introduced into Escherichia coli using the pGEX-2T expression vector to analyze the possible function of this LMM HSP under heat stress. (ncu.edu.tw)
Motivated by the need to tune the dynamics and improve the robustness of gene circuits, biological engineers have proposed various designs that mimic natural molecular feedback control mechanisms. (nature.com)
Treponemal proteins ranging in molecular weight from 37,000 daltons to 120,000 daltons were identified by sodium dodecyl sulphate-polyacrylamide gel electrophoresis and immunoblotting of phage lysate proteins. (bmj.com)
The molecular steps in the replication pathway that regulate the levels of viral gene expression are not well defined. (microbiologyresearch.org)
The course comprises molecular biology of genes, proteins, multicomponent protein complexes, and other biomolecules that provide structure and perform the organism's functions. (lu.se)

We report here that a small regulatory RNA (sRNA), Esr41, mediates LEE repression and flagellar gene activation. (nih.gov)
The Escherichia coli regulatory protein AraC regulates expression of ara genes in response to L-arabinose. (doe.gov)
Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions. (mpg.de)
Candidates for other membrane components and regulatory proteins are also identified. (embl-heidelberg.de)
These data define a regulatory mechanism for the difference in amounts of VP6 and NSP1 and provide evidence for post-transcriptional control of rotavirus gene expression mediated by the translational efficiency of individual viral mRNAs. (microbiologyresearch.org)

Analysis of Escherichia coli Mutants with a Linear Respiratory Chain. (mpg.de)

If the membrane-anchor is missing or defective, Fp and Ip subunits accumulate as soluble proteins in the cytoplasm. (lu.se)

Neither did antibody to the recombinant 37,000 dalton protein react with any treponemal proteins purified by fibronectin affinity chromatography. (bmj.com)

The canonical function of a bacterial sigma (σ) factor is to determine the gene specificity of the RNA polymerase (RNAP). (nih.gov)
Further studies would compare the different transaldolases with respect to their substrate and stereo specificity to TalB from Escherichia coli. (europa.eu)
The sensitivity and specificity of the indirect ELISA using baculovirus expressed 3AB protein as the antigen were 62.4% and 86.8-91.7%, respectively. (ncl.edu.tw)
The sensitivity and specificity of the double-sandwich blocking ELISA using E. coli expressed 3AB protein as the antigen were 94% and 98-100%, respectively. (ncl.edu.tw)

Previous studies have investigated the impact inversions have on gene expression induced inversions targeting specific genes or examine inversions between distantly related species. (bvsalud.org)
All three quinone species play distinct roles in ensuring optimal growth under aerobic and fermentative conditions in E. coli K 12. (mpg.de)

One recombinant phage was examined further and contained an insert encoding a prominent treponemal 37,000 dalton protein. (bmj.com)
There are solutions that address this problem, such as using the λ-phage GamS protein 5 or DNA containing Chi sites 6 as protective agents, or directly protecting the linear DNA by chemical modification of its ends 2 , 7 , 8 , 9 . (jove.com)
To identify Escherichia coli genes potentially regulated by filamentous phage infection, we used oligonucleotide microarrays. (lu.se)

This study has uncovered that σ 54 is a determinant of the uniform growth of uropathogenic E. coli on solid media. (nih.gov)

Cloning structural genes for Treponema pallidum immunogens and characterisation of recombinant treponemal surface protein, P2 (P2 star). (bmj.com)
Zymo-Parts: A Golden Gate Modular Cloning Toolbox for Heterologous Gene Expression in Zymomonas mobilis. (mpg.de)
Cloning of the phosphonoacetate hydrolase gene from Pseudomonas fluorescens 23F encoding a new type of carbon-phosphorus bond cleaving enzyme and its expression in Escherichia coli and Pseudomonas putida. (embl-heidelberg.de)
Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli B. (embl-heidelberg.de)

When LEE expression is activated, flagellar gene expression is down-regulated because bacterial flagella induce the immune responses of host cells at the infection stage. (nih.gov)
NSP1 (gene 5) and VP6 (gene 6) are expressed early in infection, and VP6 is expressed in excess over NSP1. (microbiologyresearch.org)
Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. (antikoerper-online.de)

Fig. 1: Photochemistry of light-oxygen-voltage receptors and sequences of proteins under study. (nature.com)
The nucleotide and deduced amino acid sequences of the phnA gene showed no significant homology with any data bank accessions. (embl-heidelberg.de)
An unusual finding is that one of the repetitive extragenic palindromic sequences actually overlaps with the reading frame of the phnE gene. (embl-heidelberg.de)
Nucleotide sequences of the fumarase (fumC) and aspartase (aspA) genes of Escherichia coli K12. (tcdb.org)

Balanced biosynthesis of major membrane components through regulated degradation of the committed enzyme of lipid A biosynthesis by the AAA protease FtsH (HflB) in Escherichia coli. (wikigenes.org)
10] "Global topology analysis of the Escherichia coli inner membrane proteome. (tcdb.org)
Ankyrins link integral membrane proteins to the spectrin-based membrane skeleton. (dana-farber.org)
Hereditary defects in membrane skeleton proteins. (dana-farber.org)
This indicates that AE1 and probably other integral membrane proteins have a 'lipid-anchoring' function. (dana-farber.org)
The membrane anchor of SQR in mammalian mitochondria and proteobacteria, such as Escherichia coli , consists of two polypeptides (SdhC and SdhD) and contains one heme group. (lu.se)
SQR in E. coli and B. subtilis can be assembled in the membrane without covalently bound FAD but are enzymatically inactive [3, 4, 6]. (lu.se)
It is unexpected that B. subtilis SQR without covalently bound FAD is not assembled in the E. coli membrane provided that all other aspects of assembly, including iron-sulfur cluster biogenesis, are functional in the heterologous system. (lu.se)

Transcriptional network response of carbon-limited Escherichia coli to transient carbon excess. (mpg.de)

Galactose is a monosaccharide consisting of 6 carbon atoms and it is an epimer of glucose, thus its transporter proteins are also SGLT1 and GLUT 2 (10, 12). (researchgate.net)
9] "Topological analysis of DcuA, an anaerobic C4-dicarboxylate transporter of Escherichia coli. (tcdb.org)

The dissociable sigma (σ) factor subunit directs the RNAP to different sets of genes to allow their expression in response to various cellular needs. (nih.gov)

Enabling anaerobic growth of Escherichia coli on glycerol in defined minimal medium using acetate as redox sink. (mpg.de)
Enforced ATP futile cycling increases specific productivity and yield of anaerobic lactate production in Escherichia coli. (mpg.de)
Under anaerobic conditions, the Cd ions were displaced by Cu(I), which associated with the protein at a ratio of 13:1. (portlandpress.com)

Extracts of the same cells produce a specific gel shift of the 110-bp DNA fragment and this fragment, immobilized on a solid support, specifically retains a single 7-kDa protein present only in cold-shocked cells that was found to be identical to F10.6 (CS7.4), the product of cspA. (unicam.it)
The acquired thermotolerant pGST-FL cells synthesized three types of HSPs, including the 76-, 73-, and 64-kDa proteins according to their abundance at a lethal temperature of 47.5°C. This finding indicates that a plant class I LMM HSP, when effectively expressed in transformed prokaryotic cells that do not normally synthesize this class of LMM HSPs, may directly or indirectly increase thermotolerance. (ncu.edu.tw)
Genes Cells, 2011 Jun. (nih.gov)
The locus of enterocyte effacement (LEE) in EHEC encodes a type three secretion system and effector proteins, all of which are essential for bacterial adherence to host cells. (nih.gov)
Esr41-mediated ler and pch repression was not observed in cells lacking hfq, which encodes an RNA-binding protein essential for most sRNA functions, indicating that Esr41 acts in an Hfq-dependent manner. (nih.gov)
The recombinant vector was then transformed into One Shot TOP10 competent Escherichia coli cells. (umsystem.edu)

In this study, the 3A and 3AB genes of O/TWN/97 were cloned using pREST, pET43 and pBlueBacHis2 plasmids. (ncl.edu.tw)

Hence, the native protein should be used for further investigations and a purification protocol for the native protein was established. (europa.eu)

alter gene expression and are associated with nucleoid protein binding sites. (bvsalud.org)

Non-structural proteins (NSP) are produced during viral replication. (ncl.edu.tw)
The inactivated vaccines consist of purified viral particle without or with only minor contaminants of NSP and thus induce antibody mainly against structural proteins of virus. (ncl.edu.tw)

In 4% of inversions representing 33 genes , differential gene expression between inverted and non-inverted homologs was detected, with greater than two-thirds (71%) of differentially expressed inverted genes having 9.4-85.6-fold higher gene expression . (bvsalud.org)

This entry represents the N-terminal domain of PhnA proteins, which is predicted to form a zinc-ribbon, found in some proteobacteria. (embl-heidelberg.de)

This cleavage can be influenced by ribosomal protein presence. (wikipedia.org)

Unique to the norVW promoter in Escherichia coli is the presence of three enhancer sites associated with a binding site for 54-RNA polymerase. (strath.ac.uk)

RNA codons and protein synthesis. (crossref.org)
IMPORTANCE RNA synthesis is the first step of gene expression. (nih.gov)
In Escherichia coli, a DNA mismatch repair (MMR) pathway corrects errors that occur during DNA replication by coordinating the excision and re-synthesis of a long tract of the newly-replicated DNA between an epigenetic signal (a hemi-methylated d(GATC) site or a single-stranded nick) and the replication error after the error is identified by protein MutS. (duke.edu)
This is a fast, easy, and direct approach for expression in cell-free protein synthesis systems using linear DNA templates. (jove.com)
Cell-free protein synthesis (CFPS) has recently become very popular in the field of synthetic biology due to its numerous advantages. (jove.com)
Cell-free protein synthesis (CFPS) systems are increasingly being used as a fast, simple, and efficient method for biosensor engineering, decentralized manufacturing, and prototyping of genetic circuits 1 . (jove.com)

Phylogenetic affiliations of mesopelagic acantharia and acantharian-like environmental 18S rRNA genes off the southern California coast. (mbl.edu)

Different activator ATPases couple diverse environmental cues to the σ 54 -RNAP to mediate adaptive changes in gene expression. (nih.gov)

Anatomy8

Organisms24

Diseases7

Chemicals and Drugs110

Analytical, Diagnostic and Therapeutic Techniques and Equipment31

Phenomena and Processes81

Disciplines and Occupations2

Technology, Industry, Agriculture1

Information Science4

Health Care4

Strains9

Sequence14

Strain4

Regulation17

Residues1

MRNA6

Plasmid7

Mammalian proteins2

Perdeuterated proteins for neutron-scattering1

Transcription8

Bacteria7

Characterization3

Encode2

Genome5

Cell division proteins2

Chromosome1

Bacillus2

Transformants2

Operon4

Fusion protein8

Genomic3

Pathways2

Lysates2

Homologous3

Molecular5

Regulatory5

Mutants1

Cytoplasm1

Affinity1

Specificity4

Species2

Phage3

Uropathogenic1

Cloning4

Infection3

Sequences4

Membrane8

Transcriptional1

Transporter2

Subunit1

Anaerobic3

Cells6

Plasmids1

Purification1

Nucleoid protein1

Structural proteins2

Differentially expressed1

Proteobacteria1

Ribosomal1

Enhancer1

Synthesis6

RRNA1

Activator1